Chemistry 522 (3 Credits) Section 16708 Winter Semester 2003

BIOCHEMISTRY II

Chemistry 522 (3 credits) Section 16708 Winter Semester 2003 Dr. Tormanen Office: Dow 347 Telephone: 3252 e-mail: torma1cd Web sites: http://www.cst.cmich.edu/users/torma1cd and http://www.cst.cmich.edu/units/chm/Facultysum/ChmTORMANEN.html Office hours: 9-11:50 am Thur and 3-3:50 pm Wed and Fri or by appointment Lecture: 9:00-9:50 am Monday, Wednesday, and Friday in Dow 136 Required textbook: Fundamentals of Biochemistry by Voet, Voet, and Pratt, $129.25 (new) $96.95 (used) Optional supplements: Take Note! and Metabolic Pathways Course prerequisite: Biochemistry I [CHM 521 (or CHM 425)]

SCHEDULE OF LECTURES

Week Date Chapter Subject 1 W Jan 8 18.1 Photosynthetic pigments F Jan 10 18.2 Light reactions and photophosphorylation

2 M Jan 13 18.3 Photosynthetic reductive pentose cycle W Jan 15 18.3 Regulation and the C-4 pathway F Jan 17 19.1-2 Activation and transport of fatty acids

3 M Jan 20 19.2 Fatty acid beta oxidation W Jan 22 19.3 Ketone body metabolism F Jan 24 19.4 Fatty acid biosynthesis

4 M Jan 27 19.5 Regulation of fatty acid metabolism W Jan 29 19.6 Biosynthesis of glycerophospholipids

F Jan 31 -- Examination I on Chapters 18 and 19.1-6

5 M Feb 3 19.6 Biosynthesis of sphingolipids W Feb 5 19.7 Biosynthesis of terpenes and cholesterol F Feb 7 19.7 Control of cholesterol metabolism

6 M Feb 10 20.1-2 Amino acid deamination W Feb 12 20.3 The urea cycle F Feb 14 20.4 Breakdown of amino acids

7 M Feb 17 20.5 Biosynthesis of nonessential amino acids W Feb 19 20.5 Biosynthesis of essential amino acids F Feb 21 20.6 Heme biosynthesis and degradation

8 M Feb 24 20.6-7 Other products of amino acid metabolism W Feb 26 -- Examination II on Chapters 19.6-7 and 20 F Feb 28 22.1 Synthesis of purine ribonucleotides

Mar 3-7 -- Spring Break Chemistry 522 SCHEDULE OF LECTURES (continued)

Week Date Chapter Subject

9 M Mar 10 22.2 Synthesis of pyrimidine ribonucleotides W Mar 12 22.3 Formation of deoxyribonucleotides F Mar 14 22.4 Nucleotide degradation and uric acid

10 M Mar 17 3.2,23.1 Nucleic acid structure W Mar 19 23.2-3 Properties of DNA and RNA F Mar 21 23.4-5 Eukaryotic chromosome structure

11 M Mar 24 24.1-2 Prokaryotic DNA replication W Mar 26 24.3 Eukaryotic DNA replication

F Mar 28 24.4-6 Mutations and DNA repair

12 M Mar 31 -- Examination III on Chapters 22 - 24 W Apr 2 25.1-2 Transcription in prokaryotes F Apr 4 25.2 Transcription in eukaryotes

13 M Apr 7 25.3 Posttranscriptional processing W Apr 9 26.1 The genetic code F Apr 11 26.2-3 Transfer RNA and ribosomes

14 M Apr 14 26.4 Polypeptide synthesis W Apr 16 27.1-2 Regulation of prokaryote gene expression F Apr 18 -- Good Friday Holiday

15 M Apr 21 27.3 Regulation of eukaryotic gene expression W Apr 23 3.5 Recombinant DNA technology F Apr 25 3.5 Genetic engineering; PCR

16 Mon Apr 28 -- Final examination on Chapters 3.5 and 8:00-9:50 am 25 - 27

Note: The Department of Biology offers an advanced molecular biology course (BIO 524) which requires CHM 522 as a prerequisite. BIO 524 includes a laboratory. CHM 522 does not include a laboratory. Students who have not already taken Biochemistry Laboratory (CHM 527) with CHM 521 may take CHM 527 during Fall Semester 2003. CHM 527 is recommended for more complete understanding of lecture material and also to gain experience in laboratory methods that are needed for graduate school, professional school or a laboratory research career. Biochemical Reactions (CHM 680) is offered in Midland from February 18 to March 25, 2003. The emphasis in CHM 680 is on the function of coenzymes and cofactors in enzymatic reaction mechanisms. GRADING

Copies of old examinations are on reserve on the third floor of Park Library and are available on the internet at CMU Libraries’ Catalog Course Reserves web page: http://catalog.lib.cmich.edu/search/r?SEARCH=chm+522 Use them as a practice test or as homework problems. Copies of old final examinations are not on reserve.

Grading scale: 90-100% A, 80-89% B, 60-79% C, 50-59% D, and 0- 49% E Distribution of points in the course: Each monthly examination will be 100 points. There will be a total of 400 points in the course. Pluses and minuses are given to those on the upper and lower ends of each grade range. Examinations must be taken during the scheduled time unless prior arrangements have been made to take the examination early. No make-up examinations will be given. If ill on the day of the examination, contact me at telephone 3252 or email torma1cd. If I cannot be reached, leave a message with the department secretary at 3981. An excused absence from one examination will not affect your course grade. An unexcused absence will result in a score of zero for that examination. All students must take the final examination. A grade of I (Incomplete) can be assigned according to the regulations on page 73 of the CMU Bulletin. After each monthly examination, a key will be posted in the glass case outside of the Biochemistry Laboratory (Dow 246). The key will remain up until the next examination.

To do well in this course, read the chapter prior to coming to class, attend all lectures, take careful notes and review them after each class, and work problems. You may find it helpful to bring two or three different colors of pens or pencils to class for taking notes. Permission is granted to tape record the lectures. No eating, drinking, visiting, or reading the newspaper is allowed while the class is in session. It is very disruptive to the class if you arrive late or leave early. Since the understanding of a lecture is dependent upon understanding the previous lecture, do not allow yourself to get behind. Biochemistry cannot be learned by trying to memorize the chapters the night before an examination. Students with disabilities requiring accommodations to participate in class activities or meet course requirements should first register with the Office of Student Disability Services (Foust Hall 250, telephone 774-3018, TDD #2568) and then contact me as soon as possible. INFORMATION ABOUT YOUR PROFESSOR

I was born and raised on a dairy farm in southern Minnesota, and I have two older brothers and one sister. I was the valedictorian of the Cokato high school class of 1964. In 1968 I graduated from the University of Minnesota in Minneapolis with a major in zoology and a minor in chemistry. My Bachelor of Arts degree was with Magna Cum Laude honors, and I was inducted into the Phi Beta Kappa honor society. After graduation I was drafted by the U.S. army and sent to South Vietnam for one year. I was an infantryman who carried a radio-telephone. Although I was in a lot of combat in the jungle and rice paddies, I did not get wounded. This was very fortunate since three out of four infantrymen in the Vietnam war either were killed or wounded. I have pleasant memories of R and Rs in Sidney, Australia and Honolulu, Hawaii. After returning from Vietnam in 1970, I was stationed for a few months in Fort Carson, Colorado, where I developed a love for the mountains. In the fall of 1970, I began graduate studies in the Biochemistry Department in the Medical School of the University of Minnesota. Also during that fall, I met Susan Wood who was teaching elementary school in my home town. We got married in June of 1971, and our first daughter Elizabeth was born in March of 1974. She graduated from Earlham College in 1996 and received a master's degree in 1999 in Environmental Policy at the Monterey Institute in California. In June of 1974, I received my Ph.D. with a major in biochemistry and a minor in chemistry. My first job with a salary of $9,500 per year was a postdoctoral researcher in the Medical School of the University of New Mexico in Albuquerque. The research was on the regulation of cholesterol metabolism. After one year of postdoctoral research, I spent two years teaching at a private liberal arts college in hot and humid east Texas. This was followed by one year of teaching at a college in smoggy and ritzy Pasadena, California. In 1978 I returned to the desert at the University of New Mexico where I spent three years as a research professor in the Biochemistry Department of the Medical School. Our second daughter Abigail was born in 1979. She graduated from Central Michigan University in 2001 and lives in San Diego, Califonia. I came to Central Michigan University in August of 1981, and I have been teaching, serving on numerous committees, and conducting research on enzymes since then. During my professional career, I have presented research papers in Minneapolis, Atlantic City, St. Louis, Dallas, Washington D.C., Amsterdam The Netherlands, Prague Czechoslovakia, Saginaw, Jerusalem Israel, San Diego, Orlando, Chicago, and Birmingham England. I have published 17 papers in journals such as the Journal of Biological Chemistry and the Journal of Inorganic Biochemistry. Besides chemistry and traveling, I am interested in astro- nomy, meteorology, nutrition, health, gardening, religion, and history. Also, I enjoy bicycling and cross-country skiing, and I collect license plates, road maps, and American Flyer toy trains.