OICR Agenda Template

qPCR and Nanostring Workshop Agenda

February 22-23rd, 2017 Venue: Ontario Institute for Cancer Research, 661 University Avenue, Toronto, M5G 0A3

Upon completion of the workshop, participants should be able to: • Apply techniques to extract RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples • Describe the difference between qPCR and Nanostring platforms and their uses • Apply methods for qPCR and analysis including how to study genes of interest • Apply data analysis techniques to normalize a dataset for Nanostring • Apply methods for Nanostring and analysis including how to generate graphs to show changes

Day One: February 22nd, 2017

Time TOPIC PRESENTER 09:45 Registration and refreshments 10:00 Introductions, Overview and Safety John Bartlett and Marta Radic 10:30 Overview of Nanostring and qPCR protocols Cheryl Crozier/Taryne Chong 10:45 Gene Expression Station: Nanostring: Dan Dion  qPCR qPCR: Cheryl Crozier/Taryne Chong  Nanostring Hybridization 12:30 Lunch 13:15 Gene Expression Station: Nanostring: Dan Dion  qPCR qPCR: Cheryl Crozier/Taryne Chong  Nanostring Hybridization 15:30 Coffee Break 15:45 qPCR vs Nanostring and Current Projects Discussion Jane Bayani and Mel Spears 17:00 Wrap up Discussion and Questions Jane Bayani 17:15 Close

Day Two: February 23rd, 2017

Time TOPIC PRESENTER 08:30 Coffee 09:00 Nanostring: Prep Station Nicola Lyttle 09:45 Nanostring Analysis with training code set Michael Jansen 12:30 Lunch 13:15 Nanostring: Digital Analyzer Nicola Lyttle 14:00 qPCR Analysis Kevin Kelly 15:00 Wrap up Discussion and Questions Jane Bayani 15:30 Close This event is an Accredited Group Learning Activity (Section 1) as defined by the Maintenance of Certification program of the Royal College of Physicians and Surgeons of Canada, approved by the Canadian Association of Pathologists-Association Canadienne des pathologistes. This event is an Accredited Group Learning Activity (Section 1) as defined by the Maintenance of Certification program of the Royal College of Physicians and Surgeons of Canada, approved by the Canadian Association of Pathologists-Association Canadienne des pathologistes.