Linking Microbial Community Structure to Β-Glucosidic Function in Soil Aggregates

Linking Microbial Community Structure to β-Glucosidic Function in Soil Aggregates

Supplemental Data

Supplemental Figure 1. Frequency distribution of β-glucosidase activities in 450 aggregates. Supplemental Table 1. Sample metadata and alpha diversity metrics β-gluc total total # of non- Sample activity Total # of # of Simpson ATP mass sequences parametric Chao2 ID (median sequences OTUs1 (1/D)2 (fmol) (mg) analyzed Shannon2 µmol/hr) 35.7 383 HAP1 69.5 138 7.66 491 350 147 4.73 (27.4,51.2) (280,566) 21.2 284 HAP2 69.5 128 5.42 739 350 129 4.40 (16.7,29.0) (214,411) 23.5 299 HAP3 67.7 124 4.55 486 350 142 4.63 (17.8,34.8) (230,421) 31.0 322 HAP4 70.3 147 4.43 358 350 147 4.74 (22.8,48.1) (246,455) 78.8 378 HAP5 69.8 120 5.49 634 350 175 5.19 (62.0,108.3) (298,510) LAP1 69.5 28 6.42 33 - nd nd nd nd 40.6 403 LAP2 70.7 27 4.58 495 350 144 4.71 (32.4,54.4) (286,615) 39.5 335 LAP3 68.6 25 6.78 567 350 141 4.67 (31.9,52.0) (250,485) 80.6 328 LAP4 67.3 24 6.98 369 350 153 4.95 (65.6,104.3) (252,461) 43.2 239 LAP5 62.3 23 4.26 416 350 116 4.43 (36.1,53.8) (179,354) 55.1 274 LAP6 68.6 28 3.68 366 350 136 4.71 (45.3,70.2) (212,387) LAP7 57.4 24 2.92 231 - nd nd nd nd 1 OTUs identified using furthest neighbor linkage clustering at ≥97% identity. 2 Calculated on the subsample of 350 sequences from each sample; values in () are the upper and lower confidence intervals Supplemental Table 2. p-values for the separation of high and low activity groups AMOVA UniFrac unweighted UniFrac weighted Sorenson 0.00797 n.d. n.d. Jaccard 0.00536 n.d. n.d. Bray-Curtis <1e-05 0.00710 <0.00010 Yue & Clayton 0.0079 0.02550 <0.00010 Morisita-Horn 0.00826 0.00910 <0.00010 Supplemental Table 3. Correlation of ATP, β-glucosidase activity and total mass with NMDS ordination distances of the high- and low- activity groups. Correlation determined using Spearman’s rank correlation coefficient and significant p-values are highlighted. Coordinate 1 Coordinate 2 Coordinate 3

Correlation p-value Correlation p-value Correlation p-value

ATP -0.03764 0.910094 -0.414039 0.214193 -0.0941 0.777714

β-glucosidase 0.418182 0.209644 -0.90303 0.000344 0.260606 0.434321 mass -0.587879 0.073878 -0.272727 0.413253 -0.672727 0.033041

Supplemental Figure 2. Biplot showing the correlation of β-glucosidase activity and total mass with the NMDS ordination of the high-activity (green) and low-activity (red) groups.