UCSF Lab Standard Operating Procedure
Chemical Process, Name or Hazard Class: Magenta Dye Compounds
Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Department: Enter text. Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Enter text.
Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class
Purpose
Magenta dye is flammable and corrosive. Keep away from sources of ignition and heat.
Harmful to respiratory tract, skin and eyes. Corrosive if inhaled or swallowed. Prolonged or repeated contact may dry skin and cause severe effects.
Some synonyms might include dimethyl fuchsin, C.I. Basic Red 9, C.I. Basic Violet 12 and C.I. Basic Violet 14.
Magenta is a dye and is used for a number of commercial applications. Is used as a laboratory reagent, microbiological stain or dye intermediate and is antifungal.
Its composition depends on the supplier and can include n-butyl acetate, butan-1-ol and 2-methoxy-1- methylethyl acetate.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: N/A
Class: Corrosive, Flammable
Molecular Formula: N/A
Form (physical state): liquid
Magenta Dye Compounds 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Color: dark pink
Boiling point: N/A
Potential Hazards/Toxicity
Magenta dye is flammable and corrosive. Keep away from sources of ignition and heat.
Harmful to respiratory tract, skin and eyes. Corrosive if inhaled or swallowed. Prolonged or repeated contact may dry skin and cause severe effects.
Vapors of Magenta dye may cause drowsiness and dizziness. Exposure to decomposition products may cause a health hazard. Serious effects may be delayed following exposure.
No permissible exposure limit data is available.
The following acute toxicity data is available:
Oral LD50 > 5000 mg/kg [rat]
Engineering Controls
Handle using a chemical fume hood with good ventilation and electrically grounded lines and equipment.
Personal Protective Equipment (PPE)
Respirator Protection If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://www.ehs.ucsf.edu/respiratory-protection-program)
Hand Protection Gloves must be worn. Use proper glove removal technique to avoid any skin contact. Nitrile gloves are recommended.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with Magenta.
Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf OR http://www.allsafetyproducts.biz/page/74172 OR http://www.showabestglove.com/site/default.aspx OR http://www.mapaglove.com/
Eye Protection ANSI approved properly fitting safety glasses or chemical splash goggles. Face shield is also recommended.
Skin and Body Protection Flame resistant/cotton lab coats must be worn and be appropriately sized for the individual and buttoned to their full length. Laboratory coat sleeves must be of sufficient length to prevent skin exposure while wearing
Magenta Dye Compounds 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) gloves. Full length pants and close-toed shoes must be worn at all times by all individuals that are occupying the laboratory area. The area of skin between the shoe and ankle should not be exposed.
Hygiene Measures Wash thoroughly and immediately after handling. Remove any contaminated clothing and wash before reuse.
First Aid Procedures
If inhaled Move into the fresh air immediately and give oxygen. If not breathing give artificial respiration. Seek medical attention immediately.
In case of skin contact Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash any contaminated clothing before reuse. Thoroughly clean shoes before reuse. Seek medical attention immediately.
In case of eye contact Check for and remove any contact lenses. Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. Seek immediate medical attention and continue eye rinse during transport to hospital.
If swallowed Do NOT induce vomiting unless directed by medical personnel. Never give anything by mouth to an unconscious person. Seek medical attention immediately.
Special Handling and Storage Requirements
Precautions for safe handling: Flammable liquid, avoid heat or sources of ignition. Avoid contact with skin and eyes and inhalation. Avoid inhalation of vapor or mist. Avoid formation of dust.
Conditions for safe storage: Flammable liquid, store away from heat or sources of ignition. Store in a flame- proof cabinet. Keep in a dry place. Keep container tightly closed in a cool, dry, and well ventilated. Keep away from incompatible materials and conditions. Keep cool and protect from sunlight.
Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only) for assistance.
Magenta Dye Compounds 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800- 222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415- 476-1300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885- 7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://www.ehs.ucsf.edu/safety-data-sheet-sds-1
Protocol/Procedure
Quantities covered by this SOP: ______(g , ml) to ______(g, ml)
Temperature range covered by this SOP: __ °C – __ °C
General Overview and Purpose:
Magenta Dye Compounds 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Enter the experimental purpose
Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Magenta Dye Compounds 5 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)