RNA Interference for the Control of Whiteflies (Bemisia Tabaci) by Oral Route

RNA interference for the control of whiteflies (Bemisia tabaci) by oral route

SANTOSH KUMAR UPADHYAY, K CHANDRASHEKAR, NIDHI THAKUR, PRAVEEN CHANDRA VERMA, J FRANCIS BORGIO, PRADHYUMNA KUMAR SINGH and RAKESH TULI J. Biosci. 36(1), March 2011, 000–000 © Indian Academy of Sciences Supplementary material

In this, we show the result of Blast analysis of 10 genes of Drossoplila with Whiteflies EST sequences.

Gene 1-

Query= gi|28573581|ref|NM_079076.3| Drosophila melanogaster Actin 57B CG10067-RA, transcript variant A (Act57B), mRNA (1726 letters)

Database: whitefly 8903 sequences; 2,688,842 total letters

Score E Sequences producing significant alignments: (bits) Value gb|EE601600.1|EE601600 BT-TYLCV-048-1-H9-T3_H09 Whitefly Bemisia... 553 e- 157 gb|EE601653.1|EE601653 BT-TYLCV-050-1-D4-T3_D04 Whitefly Bemisia... 385 e- 107 gb|EE601858.1|EE601858 BT-TYLCV-055-1-G6-T3_G06 Whitefly Bemisia... 379 e- 105 gb|EE674682.1|EE674682 BT-TYLCV-024-1-D4-T3_D04 Whitefly Bemisia... 339 3e- 093 gb|EE600399.1|EE600399 BT-TYLCV-018-1-D4-T3_D04 Whitefly Bemisia... 293 1e- 079 gb|EE600802.1|EE600802 BT-TYLCV-027-1-E1-T3_E01 Whitefly Bemisia... 280 2e- 075 gb|EE601080.1|EE601080 BT-TYLCV-033-1-F11-T3_F11 Whitefly Bemisi... 270 2e- 072 gb|EE596967.1|EE596967 BT-H-046-1-B3-T3_B03 Whitefly Bemisia tab... 258 8e- 069 gb|EE599964.1|EE599964 BT_TYLCV002_B03 Whitefly Bemisia tabaci (... 230 2e- 060 gb|EE600649.1|EE600649 BT-TYLCV-024-1-A6-T3_A06 Whitefly Bemisia... 210 2e- 054 gb|EE598970.1|EE598970 BT-TOMOV-042-1-A5-T3_A05 Whitefly Bemisia... 204 1e- 052 gb|EE597646.1|EE597646 BT-TOMOV-013-1-H7-T3_H07 Whitefly Bemisia... 176 2e- 044 gb|EE598520.1|EE598520 BT-TOMOV-032-1-F9-T3_F09 Whitefly Bemisia... 174 9e- 044 gb|EE600216.1|EE600216 BT-TYLCV-014-1-C4-T3_C04 Whitefly Bemisia... 165 9e- 041 gb|EE595819.1|EE595819 BT-H-019-1-C5-T3_C05 Whitefly Bemisia tab... 113 3e- 025 gb|EE600020.1|EE600020 BT_TYLCV003_B11 Whitefly Bemisia tabaci (... 105 7e- 023 gb|EE599931.1|EE599931 BT_TYLCV001_E07 Whitefly Bemisia tabaci (... 100 4e- 021 gb|EE602516.1|EE602516 TYLCV-BT012_H11 Whitefly Bemisia tabaci (... 96 7e- 020 gb|EE599443.1|EE599443 TOMOV-BT006_H02 Whitefly Bemisia tabaci (... 70 4e- 012 gb|EE596034.1|EE596034 BT-H-024-1-B3-T3_B03 Whitefly Bemisia tab... 66 6e- 011 gb|EE597978.1|EE597978 BT-TOMOV-021-1-B11-T3_B11 Whitefly Bemisi... 36 0.056 gb|EE598792.1|EE598792 BT-TOMOV-038-1-E5-T3_E05 Whitefly Bemisia... 30 3.5 gb|EE598851.1|EE598851 BT-TOMOV-039-1-G11-T3_G11 Whitefly Bemisi... 30 3.5 gb|EE600310.1|EE600310 BT-TYLCV-016-1-D3-T3_D03 Whitefly Bemisia... 30 3.5 gb|EE600487.1|EE600487 BT-TYLCV-020-1-C5-T3_C05 Whitefly Bemisia... 30 3.5 gb|EE602287.1|EE602287 TYLCV009_C04 Whitefly Bemisia tabaci (rea... 30 3.5 gb|EE600705.1|EE600705 BT-TYLCV-025-1-B12-T3_B12 Whitefly Bemisi... 30 3.5 gb|EE601240.1|EE601240 BT-TYLCV-037-1-E10-T3_E10 Whitefly Bemisi... 30 3.5 gb|EE601827.1|EE601827 BT-TYLCV-054-1-H2-T3_H02 Whitefly Bemisia... 30 3.5

>gb|EE601600.1|EE601600 BT-TYLCV-048-1-H9-T3_H09 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 796

Score = 553 bits (279), Expect = e-157 Identities = 586/674 (86%), Gaps = 11/674 (1%) Strand = Plus / Plus

Query: 123 gacaatggctccggcatgtgcaaggccggtttcgccggtgatgacgctccccgtgccgtc 182 |||||||| ||||| |||||||||||||| || ||||| ||||||||||||||||| ||| Sbjct: 89 gacaatggatccggtatgtgcaaggccggatt-gccggggatgacgctccccgtgctgtc 147

Query: 183 ttcccctccatcgtcggtcgtccacgccaccagggtgtgatggtcggtatgggccagaag 242 |||||||||||||||||||| ||| | || |||| ||| |||||||||||||| || || Sbjct: 148 ttcccctccatcgtcggtcggccaagacatcagg-tgtcatggtcggtatgggtcaaaaa 206

Query: 243 gactcgtacgtcggtgatgaggcccagtccaagcgtggtatcctcaccctgaaatacccc 302 ||||| ||||| ||||| || |||||||||||| |||||||||||||||||||||||| Sbjct: 207 gactcatacgtaggtgacgaagcccagtccaagaa-ggtatcctcaccctgaaatacccc 265

Query: 303 atcgagcacggtatcatcaccaactgggatgatatggagaagatctggcaccacaccttc 362 |||||||||||||||||||| ||||||||||| || || ||||||||||| ||||||||| Sbjct: 266 atcgagcacggtatcatcacaaactgggatgacat-gaaaagatctggcatcacaccttc 324

Query: 363 tacaacgagctgcgtgttgcccccgaggagcaccccgtcctgctgactgaggcccccctg 422 ||||||||||| || |||||||| || ||||||||| |||| ||||| || |||||||| Sbjct: 325 tacaacgagctccgagttgccccagaagagcacccc-tcctcctgaccgaagcccccctc 383

Query: 423 aaccccaaggctaaccgcgagaagatgacccagatcatgtttgagaccttcaactcgccc 482 ||||||||||| ||||| || |||||||| ||||||| |||||| ||||||||| | ||| Sbjct: 384 aaccccaaggccaaccgtgaaaagatgactcagatca-gtttgaaaccttcaacacaccc 442

Query: 483 gccatgtacgtcgccatccaggccgtgctctccctgtacgcctccggtcgtaccaccggt 542 ||||||||||||||||||||||| |||||||||||||| ||||||||||||||||||||| Sbjct: 443 gccatgtacgtcgccatccaggctgtgctctccctgta-gcctccggtcgtaccaccggt 501

Query: 543 atcgttctggactccggtgatggtgtctcccacaccgtccccatctatgagggttatgct 602 ||||| |||||||||| |||||||||||||||||||| |||||||| || ||||| || Sbjct: 502 atcgtcttggactccggagatggtgtctcccacaccgt-cccatctacgaaggttacgcc 560

Query: 603 ctgccccatgccatcctccgtctggatctggctggtcgcgatttgaccgactacctgatg 662 || ||||| |||||||||||| |||| |||||||||| | |||||||||||||| ||| Sbjct: 561 ctcccccacgccatcctccgtttggacttggctggtcgtg-cttgaccgactacctcatg 619

Query: 663 aagatcctgaccgagcgcggctactctttcaccaccaccgctgagcgtgaaattgtccgt 722 || ||||| || || || || ||| ||||||||||| || ||| | |||||||||||| Sbjct: 620 aaaatcctcactgaacgtggttacagcttcaccaccacagc-gagagagaaattgtccgt 678

Query: 723 gacatcaaggagaagttgtgctatgttgccctggacttcgagcaggagatggccaccgcc 782 |||||||||||||| || || || || ||| | ||||||||| |||| |||||| |||| Sbjct: 679 gacatcaaggagaaattatgttacgtcgccttagacttcgag-aggaaatggccnacgcc 737

Query: 783 gccgcctccacctc 796 || ||||||||||| Sbjct: 738 gctgcctccacctc 751

>gb|EE601653.1|EE601653 BT-TYLCV-050-1-D4-T3_D04 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 693

Query: 612 gccatcctccgtctggatctggctggtcgcgatttgaccgactacctgatgaagatcctg 671 |||||||||||| |||| |||||||||| || |||||||||||||| ||||| ||| | Sbjct: 5 gccatcctccgtttggacttggctggtcgtgacttgaccgactacctcatgaaaatc-tc 63

Query: 672 accgagcgcggctactctttcaccaccaccgctgagcgtgaaattgtccgtgacatcaag 731 || || || || ||| ||||||||||| |||||| | ||||||||||||||||||| | Sbjct: 64 actgaacgtggttacagcttcaccaccacagctgagagagaaattgtccgtgacatca-g 122

Query: 732 gagaagttgtgctatgttgccctggacttcgagcaggagatggccaccgccgccgcctcc 791 ||||| || || || || ||| | |||||||||||||| |||||||||||||| ||||| Sbjct: 123 gagaaattatgttacgtcgccttagacttcgagcaggaaatggccaccgccgctgcctc- 181

Query: 792 acctccctggagaagtcgtacgagcttcccgacggacaggtgatcaccatcggcaacgag 851 ||||| | || ||||| ||||| | |||||||||||||| ||||| ||||| ||||| Sbjct: 182 acctcattagaaaagtcctacgaattgcccgacggacaggtcatcacaatcggaaacgaa 241

Query: 852 cgtttccgttgccccgagtccctgttccagccttccttcctgggaatggaatcctgcggc 911 ||| |||||| || ||| |||| |||||||| |||||| |||| |||||||| ||||| Sbjct: 242 ggtt-ccgttgtccagaggccctcttccagccatccttcttgggtatggaatcttgcggt 300

Query: 912 atccacgagaccgtctacaactccatcatgaagtgcgatgtggatatccgtaaggacctg 971 |||||||||||||||||||||||||||||||||| || || || |||||||||||| || Sbjct: 301 -tccacgagaccgtctacaactccatcatgaagtgtgacgtcgacatccgtaaggacttg 359

Query: 972 tacgccaacatcgtcatgtccggtggcaccaccatgtaccccggtattgccgatcgtatg 1031 | |||||||| ||| ||||||| || |||||||||||||| ||||| ||||| | ||| Sbjct: 360 t-cgccaacacagtcttgtccggaggtaccaccatgtaccctggtatcgccgacagaatg 418

Query: 1032 cagaaggagatcacctccctggccccatccaccatcaagatcaagatcattgctccccca 1091 || ||||| |||||| ||||||||||||| || ||||||||||||||||| |||||||| Sbjct: 419 ca-aaggaaatcaccgccctggccccatcaacaatcaagatcaagatcatcgctcccccc 477

Query: 1092 gagcgcaaatactccgtctggatcggtggctccatcctggcctctctgtccaccttccag 1151 || | || |||||||| ||||||||||| |||||| |||| || |||||||||||||| Sbjct: 478 ga-aggaagtactccgtatggatcggtggttccatcttggcttccctgtccaccttccaa 536

Query: 1152 cagatgtggatctccaaggaggagtacgacgagtccggccctggcatcgtccaccgcaag 1211 |||| ||||||||||||| |||| |||||||| |||||||| || ||||||||||||||| Sbjct: 537 caga-gtggatctccaagcaggaatacgacgaatccggcccaggaatcgtccaccgcaag 595

Query: 1212 tg 1213 || Sbjct: 596 tg 597

>gb|EE601858.1|EE601858 BT-TYLCV-055-1-G6-T3_G06 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 712

Query: 711 gaaattgtccgtgacatcaaggagaagttgtgctatgttgccctggacttcgagcaggag 770 |||||||||||||||||||||||||| || || || || ||| | |||||||||||||| Sbjct: 3 gaaattgtccgtgacatcaaggagaaattatgttacgtcgccttagacttcgagcagga- 61

Query: 771 atggccaccgccgccgcctccacctccctggagaagtcgtacgagcttcccgacggacag 830 |||||||||||||| ||||||||||| | || ||||| ||||| | |||||||||||| Sbjct: 62 atggccaccgccgctgcctccacctcattagaaaagtcctacgaattgcccgacggacag 121

Query: 831 gtgatcaccatcggcaacgagcgtttccgttgccccgagtccctgttccagccttccttc 890 | ||||| ||||| ||||| | |||||||| || ||| |||| |||||||| |||||| Sbjct: 122 -tcatcacaatcggaaacgaaaggttccgttgtccagaggccctcttccagccatccttc 180

Query: 891 ctgggaatggaatcctgcggcatccacgagaccgtctacaactccatcatgaagtgcgat 950 |||| |||||||| ||||| ||||||||||||||||||||||||||||||||||| || Sbjct: 181 -tgggtatggaatcttgcggtatccacgagaccgtctacaactccatcatgaagtgtgac 239

Query: 951 gtggatatccgtaaggacctgtacgccaacatcgtcatgtccggtggcaccaccatgtac 1010 | || |||||||||||| |||||||||||| ||| ||||||| || ||||||||||| Sbjct: 240 g-cgacatccgtaaggacttgtacgccaacacagtcttgtccggaggtaccaccatgta- 297

Query: 1011 cccggtattgccgatcgtatgcagaaggagatcacctccctggccccatccaccatcaag 1070 |||||||| ||||| | ||||||||||| |||||| ||||||||||||| || |||||| Sbjct: 298 cccggtatcgccgacagaatgcagaaggaaatcaccgccctggccccatcaacaatcaag 357

Query: 1071 atcaagatcattgctcccccagagcgcaaatactccgtctggatcggtggctccatcctg 1130 ||| ||||||| |||||||| || | || |||||||| ||||||||||| |||||| || Sbjct: 358 atc-agatcatcgctccccccgaaaggaagtactccgtatggatcggtggttccatcttg 416

Query: 1131 gcctctctgtccaccttccagcagatgtggatctccaaggaggagtacgacgagtccggc 1190 || || |||||||||||||| |||||||||||||||||| |||| |||||||| |||||| Sbjct: 417 gcttc-ctgtccaccttccaacagatgtggatctccaagcaggaatacgacgaatccggc 475

Query: 1191 cctggcatcgtccaccgcaagtgcttctaag 1221 || || ||||||||||||||||| ||||||| Sbjct: 476 ccagg-atcgtccaccgcaagtgtttctaag 505

Score = 339 bits (171), Expect = 3e-093 Identities = 403/471 (85%), Gaps = 7/471 (1%) Strand = Plus / Plus

Query: 711 gaaattgtccgtgacatcaaggagaagttgtgctatgttgccctggacttcgagcaggag 770 |||||||||||||||||||||||||| || || || || ||| | |||||||||||||| Sbjct: 2 gaaattgtccgtgacatcaaggagaaattatgttacgtcgccttagacttcgagcagga- 60

Query: 771 atggccaccgccgccgcctccacctccctggagaagtcgtacgagcttcccgacggacag 830 |||||||||||||| ||||||||||| | || ||||| ||||| | |||||||||||| Sbjct: 61 atggccaccgccgctgcctccacctcattagaaaagtcctacgaattgcccgacggacag 120

Query: 831 gtgatcaccatcggcaacgagcgtttccgttgccccgagtccctgttccagccttccttc 890 | ||||| ||||| ||||| | |||||||| || ||| |||| |||||||| |||||| Sbjct: 121 -tcatcacaatcggaaacgaaaggttccgttgtccagaggccctcttccagccatccttc 179

Query: 891 ctgggaatggaatcctgcggcatccacgagaccgtctacaactccatcatgaagtgcgat 950 |||| |||||||| ||||| ||||||||||||||||||||||||||||||||||| || Sbjct: 180 -tgggtatggaatcttgcggtatccacgagaccgtctacaactccatcatgaagtgtgac 238

Query: 951 gtggatatccgtaaggacctgtacgccaacatcgtcatgtccggtggcaccaccatgtac 1010 || || |||||||||||| |||||||||||| ||| ||||||| || ||||||||||| Sbjct: 239 gt-gacatccgtaaggacttgtacgccaacacagtcttgtccggaggtaccaccatgta- 296

Query: 1011 cccggtattgccgatcgtatgcagaaggagatcacctccctggccccatccaccatcaag 1070 ||| |||| ||||| | ||||||||||| |||||| ||||||||||||| || |||||| Sbjct: 297 ccctgtatcgccgacagaatgcagaaggaaatcaccgccctggccccatcaacaatcaag 356

Query: 1071 atcaagatcattgctcccccagagcgcaaatactccgtctggatcggtggctccatcctg 1130 ||| ||||||| |||||||| || | || |||||||| ||||||||||| |||||| || Sbjct: 357 atc-agatcatcgctccccccgaaaggaagtactccgtatggatcggtggttccatcttg 415

Query: 1131 gcctctctgtccaccttccagcagatgtggatctccaaggaggagtacgac 1181 || || |||||||||||||| |||||||||||||||||| |||| |||||| Sbjct: 416 gcttc-ctgtccaccttccaacagatgtggatctccaagcaggaatacgac 465

Query: 771 atggccaccgccgcc-gcctccacctccctggagaagtcgtacgagcttcccgacggaca 829 ||||||||||||| | |||||| |||| | || ||||| ||||| | ||||||||| | Sbjct: 3 atggccaccgccgactgcctcctcctcattagaaaagtcctacgaattgcccgacgga-a 61

Query: 830 ggtgatcaccatcggcaacgagcgtttccgttgccccgagtccctgttccagccttcctt 889 ||| ||||| ||||| ||||| | |||||||| || ||| |||| |||||||| |||| Sbjct: 62 ggtcatcacaatcggaaacgaaaggttccgttgtccagaggccctcttccagccatcct- 120

Query: 890 cctgggaatggaatcctgcggcatccacgagaccgtctacaactccatcatgaagtgcga 949 | |||| |||||||| ||||| ||||||||||||||||||||||||||||||||||| || Sbjct: 121 cttgggtatggaatcttgcggtatccacgagaccgtctacaactccatcatgaagtgtga 180

Query: 950 tgtggatatccgtaaggacctgtacgccaacatcgtcatgtccggtggcaccaccatgta 1009 || || |||||||||||| |||||||||||| ||| ||||||| || ||||||||||| Sbjct: 181 -gtcgacatccgtaaggacttgtacgccaacacagtcttgtccggaggtaccaccatgta 239

Query: 1010 ccccggtattgccgatcgtatgcagaaggagatcacctccctggccccatccaccatcaa 1069 || ||||| ||||| | ||||||||||| |||||| ||||||||||||| || ||||| Sbjct: 240 -cctggtatcgccgacagaatgcagaaggaaatcaccgccctggccccatcaacaatcaa 298

Query: 1070 gatcaagatcattgctcccccagagcgcaaatactccgtctggatcggtggctccatcct 1129 || ||||||||| |||||||| || | || |||||||| ||||||||||| |||||| | Sbjct: 299 ga-caagatcatcgctccccccgaaaggaagtactccgtatggatcggtggttccatctt 357

Query: 1130 ggcctctctgtccaccttccagcagatgtggatctccaaggaggagtacgacgagtccgg 1189 || ||| |||||||||||||| |||||||||||||||||| |||| |||||||| ||||| Sbjct: 358 gg-ctccctgtccaccttccaacagatgtggatctccaagcaggaatacgacgaatccgg 416

Query: 1190 ccctggcatcgtccaccgcaagtgcttctaag 1221 ||| | ||||||||||||||||| ||||||| Sbjct: 417 ccc-agaatcgtccaccgcaagtgtttctaag 447

>gb|EE600802.1|EE600802 BT-TYLCV-027-1-E1-T3_E01 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 704

Query: 855 ttccgttgccccgagtccctgttccagccttccttcctgggaatggaatcctgcggcatc 914 |||||||| || ||| |||| |||||||| |||||| |||| |||||||| ||||| ||| Sbjct: 2 ttccgttgtccagaggccctcttccagccatccttcttgggtatggaatcttgcggtatc 61

Query: 915 cacgagaccgtctacaactccatcatgaagtgcgatgtggatatccgtaaggacctgtac 974 ||||||||||||||||||||||||||||||| || || || |||||||||||| ||||| Sbjct: 62 -acgagaccgtctacaactccatcatgaagtgtgacgtcgacatccgtaaggacttgtac 120

Query: 975 gccaacatcgtcatgtccggtggcaccaccatgtaccccggtattgccgatcgtatgcag 1034 |||||| ||| ||||||| || |||||||||||||| ||||| ||||| | |||||| Sbjct: 121 -ccaacacagtcttgtccggaggtaccaccatgtaccctggtatcgccgacagaatgcag 179

Query: 1035 aaggagatcacctccctggccccatccaccatcaagatcaagatcattgctcccccagag 1094 |||| |||||| ||||||||||||| || ||||||||||||||||| |||||||| || Sbjct: 180 -aggaaatcaccgccctggccccatcaacaatcaagatcaagatcatcgctccccccgaa 238

Query: 1095 cgcaaatactccgtctggatcggtggctccatcctggcctctctgtccaccttccagcag 1154 | || |||||||| ||||||||||| |||||| |||| || |||||||||||||| ||| Sbjct: 239 ag-aagtactccgtatggatcggtggttccatcttggcttccctgtccaccttccaacag 297

Query: 1155 atgtggatctccaaggaggagtacgacgagtccggccctggcatcgtccaccgcaagtgc 1214 ||| ||||||||||| |||| |||||||| |||||||| || ||||| ||||||||||| Sbjct: 298 atg-ggatctccaagcaggaatacgacgaatccggcccaggaatcgttcaccgcaagtgt 356

Query: 1215 ttcta 1219 ||||| Sbjct: 357 ttcta 361

>gb|EE601080.1|EE601080 BT-TYLCV-033-1-F11-T3_F11 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 664

Query: 907 gcggcatccacgagaccgtctacaactccatcatgaagtgcgatgtggatatccgtaagg 966 |||| ||||||||||||||||||||||||||||||||||| || || || |||||||||| Sbjct: 1 gcggtatccacgagaccgtctacaactccatcatgaagtgtgacgtcgacatccgtaagg 60

Query: 967 acctgtacgccaacatcgtcatgtccggtggcaccaccatgtaccccggtattgccgatc 1026 | |||||||||||| ||| ||||||| || |||||||||||||| ||||| ||||| Sbjct: 61 -cttgtacgccaacacagtcttgtccggaggtaccaccatgtaccctggtatcgccgaca 119

Query: 1027 gtatgcagaaggagatcacctccctggccccatccaccatcaagatcaagatcattgctc 1086 | ||||||||||| |||||| ||||||||||||| || ||||||||||||||||| |||| Sbjct: 120 g-atgcagaaggaaatcaccgccctggccccatcaacaatcaagatcaagatcatcgctc 178

Query: 1087 ccccagagcgcaaatactccgtctggatcggtggctccatcctggcctctctgtccacct 1146 |||| || | || |||||||| ||||||||||| |||||| |||| || |||||||||| Sbjct: 179 cccc-gaaaggaagtactccgtatggatcggtggttccatcttggcttccctgtccacct 237

Query: 1147 tccagcagatgtggatctccaaggaggagtacgacgagtccggccctggcatcgtccacc 1206 |||| |||||||||||||||||| |||| |||||||| |||||||| || |||||||||| Sbjct: 238 tcca-cagatgtggatctccaagcaggaatacgacgaatccggcccaggaatcgtccacc 296

Query: 1207 gcaa 1210 |||| Sbjct: 297 gcaa 300

>gb|EE596967.1|EE596967 BT-H-046-1-B3-T3_B03 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 423 aaccccaaggctaaccgcgagaagatgacccagatcatgtttgagaccttcaactcgccc 482 ||||||||||| ||||| || |||||||| |||||||||||||| ||||||||| | || Sbjct: 4 aaccccaaggccaaccgtgaaaagatgactcagatcatgtttgaaaccttcaacacacc- 62

Query: 483 gccatgtacgtcgccatccaggccgtgctctccctgtacgcctccggtcgtaccaccggt 542 ||||||||||||||||||||||| ||||||||||||||||||||||||||||||||| || Sbjct: 63 gccatgtacgtcgccatccaggctgtgctctccctgtacgcctccggtcgtaccacc-gt 121

Query: 543 atcgttctggactccggtgatggtgtctcccacaccgtccccatctatgagggttatgct 602 ||||| |||||||||| ||||||||||||||||||||||||||||| || ||||| || Sbjct: 122 atcgtcttggactccggagatggtgtctcccacaccgtccccatctacgaaggttacgc- 180

Query: 603 ctgccccatgccatcctccgtctggatctggctggtcgcgatttgaccgactacctgatg 662 || ||||| |||||||||||| |||| |||||||||| || |||||||||||||| ||| Sbjct: 181 ctcccccacgccatcctccgtttggacttggctggtcgtgacttgaccgactacctcatg 240

Query: 663 aa 664 || Sbjct: 241 aa 242

>gb|EE599964.1|EE599964 BT_TYLCV002_B03 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 225 gtcggtatgggccagaaggactcgtacgtcggtgatgaggcccagtccaagcgtggtatc 284 ||||||||||| || || ||||| ||||| ||||| || |||||||||||| | |||||| Sbjct: 1 gtcggtatgggtcaaaaagactcatacgtaggtgacgaagcccagtccaagagaggtatc 60

Query: 285 ctcaccctgaaataccccatcgagcacggtatcatcaccaactgggatgatatggagaag 344 ||||||||||||||||||||||||||||||||||||| ||||||||||| ||||| ||| Sbjct: 61 -tcaccctgaaataccccatcgagcacggtatcatcacaaactgggatgacatggaaaag 119

Query: 345 atctggcaccacaccttctacaacgagctgcgtgttgcccccgaggagcaccccgtcctg 404 | |||||| |||||||||||||||||||| || |||||||| || ||||||||| |||| Sbjct: 120 a-ctggcatcacaccttctacaacgagctccgagttgccccagaagagcaccccatcctc 178

Query: 405 ctgactgaggcccccctgaaccccaaggctaaccgcgagaagatgacccagatcatgttt 464 || || || |||||||| ||||||||||| ||||| || |||||||| |||||||||||| Sbjct: 179 ct-accgaagcccccctcaaccccaaggccaaccgtgaaaagatgactcagatcatgttt 237

Query: 465 gagaccttcaac 476 || ||||||||| Sbjct: 238 ga-accttcaac 248

>gb|EE600649.1|EE600649 BT-TYLCV-024-1-A6-T3_A06 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 361

Query: 779 cgccgccgcctccacctccctggagaagtcgtacgagcttcccgacggacaggtgatcac 838 |||||| ||||||||||| | || ||||| ||||| | |||||||||||||| ||||| Sbjct: 1 cgccgctgcctccacctcattagaaaagtcctacgaattgcccgacggacaggtcatcac 60

Query: 839 catcggcaacgagcgtttccgttgccccgagtccctgttccagccttccttcctgggaat 898 ||||| ||||| | |||||||| || ||| |||| |||||||| |||||| |||| || Sbjct: 61 -atcggaaacgaaaggttccgttgtccagaggccctcttccagccatccttcttgggtat 119

Query: 899 ggaatcctgcggcatccacgagaccgtctacaactccatcatgaagtgcgatgtggatat 958 | |||| ||||| ||||||||||||||||||||||||||||||||||| || || | || Sbjct: 120 g-aatcttgcggtatccacgagaccgtctacaactccatcatgaagtgtgacgtccacat 178

Query: 959 ccgtaaggacctgtacgccaacatcgtcatgtccggtggcaccaccatgtaccccggtat 1018 || ||||||| |||||||||||| ||| ||||||| || |||||||||||||| ||||| Sbjct: 179 cc-taaggacttgtacgccaacacagtcttgtccggaggtaccaccatgtaccctggtat 237

Query: 1019 tgccgatcgtatgcagaaggagatcacctccctggccccatccaccatcaagatcaagat 1078 | ||| | ||||||||||| |||||| ||||||||||||| || ||| |||||||||| Sbjct: 238 cg-cgacagaatgcagaaggaaatcaccgccctggccccatcaacaatctagatcaagat 296

Query: 1079 cattgctcccccagagcgcaaatactccgtctggatcggtggctccatcctggcctctct 1138 || | ||||||| || | || |||||||| ||||||||||| |||||| |||| || || Sbjct: 297 ca-tcctccccccgaaaggaagtactccgtatggatcggtggttccatcttggcttccct 355

Query: 1139 gtccac 1144 |||||| Sbjct: 356 gtccac 361

>gb|EE598970.1|EE598970 BT-TOMOV-042-1-A5-T3_A05 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 915 cacgagaccgtctacaactccatcatgaagtgcgatgtggatatccgtaaggacctgtac 974 |||||||||||||||||||||||||||||||| || || || |||||||||||| ||||| Sbjct: 1 cacgagaccgtctacaactccatcatgaagtgtgacgtcgacatccgtaaggacttgtac 60

Query: 975 gccaacatcgtcatgtccggtggcaccaccatgtaccccggtattgccgatcgtatgcag 1034 |||||| ||| ||||||| || |||||||||||||| ||||| ||||| | |||||| Sbjct: 61 -ccaacacagtcttgtccggaggtaccaccatgtaccctggtatcgccgacagaatgcag 119

Query: 1035 aaggagatcacctccctggccccatccaccatcaagatcaagatcattgctcccccagag 1094 |||| |||||| ||||||||||||| || ||||||||||||||||| |||||||| || Sbjct: 120 -aggaaatcaccgccctggccccatcaacaatcaagatcaagatcatcgctccccccgaa 178

Query: 1095 cgcaaatactccgtctggatcggtggctccatcctggcctctctgtccaccttccagcag 1154 | || |||||||| ||||||||||| |||||| |||| || |||||||||||||| ||| Sbjct: 179 ag-aagtactccgtatggatcggtggttccatcttggcttccctgtccaccttccaacag 237

Query: 1155 atgtggatctccaag 1169 ||| ||||||||||| Sbjct: 238 atg-ggatctccaag 251

>gb|EE597646.1|EE597646 BT-TOMOV-013-1-H7-T3_H07 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 834 atcaccatcggcaacgagcgtttccgttgccccgagtccctgttccagccttccttcctg 893 ||||| ||||| ||||| | |||||||| || ||| |||| |||||||| |||||| || Sbjct: 4 atcacaatcggaaacgaaaggttccgttgtccagaggccctcttccagccatccttc-tg 62

Query: 894 ggaatggaatcctgcggcatccacgagaccgtctacaactccatcatgaagtgcgatgtg 953 || |||||||| ||||| ||||||||||||||||||||||||||||||||||| || | Sbjct: 63 ggtatggaatcttgcggtatccacgagaccgtctacaactccatcatgaagtgtgacg-c 121

Query: 954 gatatccgtaaggacctgtacgccaacatcgtcatgtccggtggcaccaccatgtacccc 1013 || |||||||||||| |||||||||||| | | ||||||| || ||||||||||| ||| Sbjct: 122 gacatccgtaaggacttgtacgccaacacaggcttgtccggaggtaccaccatgta-ccc 180

Query: 1014 ggtattgccgatcgtatgcagaaggagatcacctccctggccccatccaccatcaagatc 1073 ||||| ||||| | ||||||||||| |||||| ||||||||||||| || ||||||||| Sbjct: 181 ggtatcgccgacagaatgcagaaggaaatcaccgccctggccccatcaacaatcaagatc 240

Query: 1074 a 1074 | Sbjct: 241 a 241

>gb|EE598520.1|EE598520 BT-TOMOV-032-1-F9-T3_F09 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 1002 accatgtaccccggtattgccgatcgtatgcagaaggagatcacctccctggccccatcc 1061 ||||||||||| ||||| ||||| | ||||||||||| |||||| ||||||||||||| Sbjct: 1 accatgtaccctggtatcgccgacagaatgcagaaggaaatcaccgccctggccccatcg 60

Query: 1062 accatcaagatcaagatcattgctcccccagagcgcaaatactccgtctggatcggtggc 1121 | ||||||||||||||||| |||||||| || | || |||||||| ||||||||||| Sbjct: 61 -caatcaagatcaagatcatcgctccccccgaaaggaagtactccgtatggatcggtggt 119

Query: 1122 tccatcctggcctctctgtccaccttccagcagatgtggatctccaaggaggagtacgac 1181 || ||| |||| || |||||||||||||| |||||||||||||||||| |||| |||||| Sbjct: 120 tc-atcttggcttccctgtccaccttccaacagatgtggatctccaagcaggaatacgac 178

Query: 1182 gagtccggccctggcatcgtccaccgcaagtgcttctaag 1221 || |||||||| || ||||| ||||||||||| ||||||| Sbjct: 179 ga-tccggcccaggaatcgttcaccgcaagtgtttctaag 217

>gb|EE600216.1|EE600216 BT-TYLCV-014-1-C4-T3_C04 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 567

Query: 1041 atcacctccctggccccatccaccatcaagatcaagatcattgctcccccagagcgcaaa 1100 |||||| ||||||||||||| || ||||||||||||||||| |||||||| || | || Sbjct: 5 atcaccgccctggccccatcaacaatcaagatcaagatcatcgctccccccgaaag-aag 63

Query: 1101 tactccgtctggatcggtggctccatcctggcctctctgtccaccttccagcagatgtgg 1160 |||||||| ||||||||||| |||||| |||| || |||||||||||||| |||||| || Sbjct: 64 tactccgtatggatcggtggttccatcttggcttccctgtccaccttccaacagatg-gg 122

Query: 1161 atctccaaggaggagtacgacgagtccggccctggcatcgtccaccgcaagtgcttcta 1219 ||||||||| |||| |||||||| |||||||| || ||||||||||||||||| ||||| Sbjct: 123 atctccaagcaggaatacgacgaatccggcccaggaatcgtccaccgcaagtgtttcta 181

>gb|EE595819.1|EE595819 BT-H-019-1-C5-T3_C05 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 516 ctgtacgcctccggtcgtaccaccggtatcgttctggactccggtgatggtgtctcccac 575 |||||||| || |||||||||||||||||||| || ||||| || |||||||| | |||| Sbjct: 5 ctgtacgcttctggtcgtaccaccggtatcgtgcttgactctggagatggtgt-ttccac 63

Query: 576 accgtccccatctatgagggttatgctctgccccatgccatcctccgtctggatctggct 635 || ||||| |||||||| ||||||||| | || ||||| ||||| ||| |||| || ||| Sbjct: 64 acagtcccaatctatgaaggttatgctttacctcatgctatccttcgtttgga-cttgct 122

Query: 636 ggtcgcgatttgaccgactacctgatgaa 664 |||||||| |||||||||||||| ||||| Sbjct: 123 ggtcgcgacttgaccgactacctcatgaa 151

>gb|EE600020.1|EE600020 BT_TYLCV003_B11 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 1101 tactccgtctggatcggtggctccatcctggcctctctgtccaccttccagcagatgtgg 1160 |||||||| ||||||||||| |||||| || | || |||||||||||| | ||||||||| Sbjct: 14 tactccgtatggatcggtggttccatcttgtcttccctgtccaccttcaa-cagatgtgg 72

Query: 1161 atctccaaggaggagtacgacgagtccggccctggcatcgtccaccgca 1209 ||||||||| |||| |||||||| |||||||| || ||||||||||||| Sbjct: 73 atctccaagcaggaatacgacgaatccggcccaggaatcgtccaccgca 121

>gb|EE599931.1|EE599931 BT_TYLCV001_E07 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Score = 99.6 bits (50), Expect = 4e-021 Identities = 137/162 (84%), Gaps = 3/162 (1%) Strand = Plus / Plus

Query: 690 ttcaccaccaccgctgagcgtgaaattgtccg-tgacatcaaggagaagttgtgctatgt 748 ||||||||||| |||||| | ||||||||||| ||||||||||||||| || || || || Sbjct: 1 ttcaccaccacagctgagagagaaattgtccgctgacatcaaggagaaattatgttacgt 60

Query: 749 tgccctggacttcgagcaggagatggccaccgccgccgcctccacctccctggagaagtc 808 ||| | |||||||||||||| |||||||||||||| ||||||||||| | || ||||| Sbjct: 61 -gccttagacttcgagcaggaaatggccaccgccgctgcctccacctcattagaaaagtc 119

Query: 809 gtacgagcttcccgacggacaggtgatcaccatcggcaacga 850 |||| | |||||||||||||| ||||| ||||| ||||| Sbjct: 120 -cacgaattgcccgacggacaggtcatcacaatcggaaacga 160

Gene 2

Query= gi|24644733|ref|NM_057424.3| Drosophila melanogaster alpha-Tubulin at 84B CG1913-RA (alphaTub84B), mRNA (1834 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE601404.1|EE601404 BT-TYLCV-044-1-F11-T3_F11 Whitefly Bemisi... 400 e- 112 gb|EE601044.1|EE601044 BT-TYLCV-032-1-H12-T3_H12 Whitefly Bemisi... 387 e- 107 gb|EE601397.1|EE601397 BT-TYLCV-044-1-E11-T3_E11 Whitefly Bemisi... 339 3e- 093 gb|EE599360.1|EE599360 TOMOV-BT005_D06 Whitefly Bemisia tabaci (... 204 1e- 052 gb|EE598896.1|EE598896 BT-TOMOV-040-1-F1-T3_F01 Whitefly Bemisia... 202 4e- 052 gb|EE598061.1|EE598061 BT-TOMOV-022-1-H11-T3_H11 Whitefly Bemisi... 182 4e- 046 gb|EE598934.1|EE598934 BT-TOMOV-041-1-D9-T3_D09 Whitefly Bemisia... 182 4e- 046 gb|EE598415.1|EE598415 BT-TOMOV-030-1-D2-T3_D02 Whitefly Bemisia... 165 1e- 040 gb|EE597467.1|EE597467 HBT010_A04_T3_024 Whitefly Bemisia tabaci... 129 5e- 030 gb|EE599287.1|EE599287 TOMOV-BT004_B11 Whitefly Bemisia tabaci (... 127 2e- 029 gb|EE595673.1|EE595673 BT-H-016-1-D3-T3_D03 Whitefly Bemisia tab... 113 3e- 025 gb|EE600920.1|EE600920 BT-TYLCV-030-1-A10-T3_A10 Whitefly Bemisi... 58 2e- 008 gb|EE599290.1|EE599290 TOMOV-BT004_C05 Whitefly Bemisia tabaci (... 54 3e- 007 gb|EE603466.1|EE603466 BT-HINST-016-1-G3-T3_G03 Whitefly Bemisia... 38 0.015 gb|EE597425.1|EE597425 HBT009_B05_T3_044 Whitefly Bemisia tabaci... 32 0.93 gb|EE598048.1|EE598048 BT-TOMOV-022-1-E9-T3_E09 Whitefly Bemisia... 32 0.93 gb|EE600663.1|EE600663 BT-TYLCV-024-1-D2-T3_D02 Whitefly Bemisia... 32 0.93 gb|EE597667.1|EE597667 BT-TOMOV-014-1-D4-T3_D04 Whitefly Bemisia... 30 3.7 gb|EE600209.1|EE600209 BT-TYLCV-014-1-B3-T3_B03 Whitefly Bemisia... 30 3.7

Query: 1089 cttcgagccggccaaccagatggtcaagtgcgatccccgtcacggcaagtacatggcctg 1148 ||||||||| ||||||||||||||||||||||| |||||||||||||| ||||||||||| Sbjct: 1 cttcgagcctgccaaccagatggtcaagtgcgacccccgtcacggcaaatacatggcctg 60

Query: 1149 ctgcatgctgtaccgcggtgatgttgtgcccaaggacgtcaacgccgctattgccaccat 1208 |||||| ||||||| || ||||||||||||||||| ||||| || || || |||||||| Sbjct: 61 -tgcatgttgtaccgaggagatgttgtgcccaaggatgtcaatgctgccatcgccaccat 119

Query: 1209 caagaccaagcgcaccattcaattcgtcgactggtgccccactggcttcaaggttggcat 1268 | |||||||| | ||||| ||||||||||||||||| |||||||| |||||||| || || Sbjct: 120 c-agaccaagagaaccatccaattcgtcgactggtgtcccactggtttcaaggtcggtat 178

Query: 1269 caactaccagccacccaccgtggtgcctggaggtgatttggccaaggtgcagcgtgccgt 1328 | ||||||| |||||||| || || ||||| || ||| | |||||||| || | ||||| Sbjct: 179 c-actaccaaccacccacagttgttcctggtggcgatcttgccaaggtccaaagagccgt 237

Query: 1329 gtgcatgttgtccaacaccacggccatcgccgaggcctgggcccgtctggaccacaagtt 1388 || ||||||||||||||||| |||||||| || || ||||||||||| ||||||||||| Sbjct: 238 atg-atgttgtccaacaccaccgccatcgctgaagcttgggcccgtcttgaccacaagtt 296

Query: 1389 cgatctgatgtacgccaagcgtgccttcgtccactggtacgttggtgagggtatggagga 1448 |||| ||||||||||||||||||| |||||||||||||||||||| || |||||||| || Sbjct: 297 cgat-tgatgtacgccaagcgtgctttcgtccactggtacgttggagaaggtatggaaga 355

Query: 1449 gggagagttctccgaggcccgtgaggatttggctgccctcgagaaggactacgaggaggt 1508 ||||| |||||||| ||||||||||| ||||||||||||||||||||||||||||| || Sbjct: 356 aggaga-ttctccgaagcccgtgaggacttggctgccctcgagaaggactacgaggaagt 414

Query: 1509 cggcatggactccggtgacggcgagggtga 1538 ||||| ||||||| ||| || |||||||| Sbjct: 415 tggcat-gactccgttgaaggagagggtga 443

Query: 578 tggccgatcagtgcaccggtctgcagggcttcctcatcttccactcgttcggtggaggta 637 ||||||| || || |||||||| ||||| ||||||||||||||||| |||||||| |||| Sbjct: 6 tggccgaccaatgtaccggtctccagggtttcctcatcttccactccttcggtgg-ggta 64

Query: 638 ccggctccggcttcacctcgctgctgatggagcgtctctccgtggactacggcaaga-ag 696 |||| ||||| |||||||| || || ||||| |||||||| || |||||||| |||| || Sbjct: 65 ccggatccggattcacctcccttctcatggaacgtctctcagtcgactacggaaagagag 124

Query: 697 tccaagctggagttcgccatctacccagccccccaggtgtccactgccgtggtcgagccc 756 | || |||| ||||| |||||||||| || ||||| ||||| || || ||||| || Sbjct: 125 t--aaattggaattcgcagtctacccagcacctcaggtatccaccgctgttgtcgaacc- 181

Query: 757 tacaactccatcctgaccacccacaccaccctggagcattccgactgcgccttcatggtc 816 |||||||| ||||| ||||||||||||||| |||| || |||||||||||||||||||| Sbjct: 182 tacaactcaatcctcaccacccacaccaccttggaacactccgactgcgccttcatggt- 240

Query: 817 gacaacgaggctatctacgacatctgccgccgcaatctggacattgagcgccccacgtac 876 ||||| || || ||||| || ||||||||||| || ||||||||||||||||||| ||| Sbjct: 241 gacaatgaagccatctatgatatctgccgccgtaacttggacattgagcgccccacctac 300

Query: 877 accaacctgaaccgtctgattggccagatcgtgtcctcgattaccgcctctctgcgattc 936 ||||||| ||| | || ||||| || ||||| ||||| || || || || || |||||| Sbjct: 301 -ccaacctcaacaggctcattggtcaaatcgtctcctccatcacagcttccctccgattc 359

Query: 937 gatggtgcccttaacgtggatctgactgagttccagaccaacttggtgccctacccacgt 996 | |||||||| ||||| || ||||||| |||||||| |||||||| || ||||||||| Sbjct: 360 gc-ggtgccctcaacgtcgacttgactgaattccagactaacttggtcccatacccacgt 418

Query: 997 attcacttccctctggtgacctacgcccccgttatctccgccgagaaggcctaccacgag 1056 || |||||||||||||| ||||| ||||| || |||||||| || |||||||| || || Sbjct: 419 at-cacttccctctggtcacctatgcccctgtcatctccgcagaaaaggcctatcatgaa 477

Query: 1057 cagctgtcggtggctgagatcaccaacgcctgcttcgagccggccaaccagatggtcaag 1116 ||| |||| || || |||||||||||||||||||||||||| |||||||||||||||||| Sbjct: 478 cag-tgtctgtcgccgagatcaccaacgcctgcttcgagcctgccaaccagatggtcaag 536

Query: 1117 tgcgatccccgtcacggcaagtacatggcctgctgcatgctgtaccgcggtgatgttgtg 1176 |||| |||||||||||||| ||||||||||| |||||| ||||||| || ||||||||| Sbjct: 537 tgcg-cccccgtcacggcaaatacatggcctgttgcatgttgtaccgaggagatgttgtg 595

Query: 1177 cccaag 1182 |||||| Sbjct: 596 cccaag 601

>gb|EE601397.1|EE601397 BT-TYLCV-044-1-E11-T3_E11 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 393

Query: 1089 cttcgagccggccaaccagatggtcaagtgcgatccccgtcacggcaagtacatggcctg 1148 ||||||||| ||||||||||||||||||||||| |||||||||||||| ||||||||||| Sbjct: 1 cttcgagcctgccaaccagatggtcaagtgcgacccccgtcacggcaaatacatggcctg 60

Query: 1149 ctgcatgctgtaccgcggtgatgttgtgcccaaggacgtcaacgccgctattgccaccat 1208 |||||| ||||||| || ||||||||||||||||| ||||| || || || |||||||| Sbjct: 61 -tgcatgttgtaccgaggagatgttgtgcccaaggatgtcaatgctgccatcgccaccat 119

Query: 1209 caagaccaagcgcaccattcaattcgtcgactggtgccccactggcttcaaggttggcat 1268 | |||||||| | ||||| ||||||||||||||||| |||||||| |||||||| || || Sbjct: 120 c-agaccaagagaaccatccaattcgtcgactggtgtcccactggtttcaaggtcggtat 178

Query: 1269 caactaccagccacccaccgtggtgcctggaggtgatttggccaaggtgcagcgtgccgt 1328 | ||||||| |||||||| || || ||||| || ||| | |||||||| || | ||||| Sbjct: 179 c-actaccaaccacccacagttgttcctggtggcgatcttgccaaggtccaaagagccgt 237

Query: 1329 gtgcatgttgtccaacaccacggccatcgccgaggcctgggcccgtctggaccacaagtt 1388 || ||||||||||||||||| |||||||| || || ||||||||||| ||||||||||| Sbjct: 238 atg-atgttgtccaacaccaccgccatcgctgaagcttgggcccgtcttgaccacaagtt 296

Query: 1389 cgatctgatgtacgccaagcgtgccttcgtccactggtacgttggtgagggtatggagga 1448 |||| ||||||||||||||||||| |||||||||||||||||||| || |||||||| || Sbjct: 297 cgat-tgatgtacgccaagcgtgctttcgtccactggtacgttggagaaggtatggaaga 355

Query: 1449 gggagagttctccgaggcccgtgagga 1475 ||||| |||||||| ||||||||||| Sbjct: 356 aggaga-ttctccgaagcccgtgagga 381

>gb|EE599360.1|EE599360 TOMOV-BT005_D06 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 959 tgactgagttccagaccaacttggtgccctacccacgtattcacttccctctggtgacct 1018 ||||||| |||||||| |||||||| || ||||||||||| ||||||| |||||| |||| Sbjct: 14 tgactgaattccagactaacttggtcccatacccacgtatccacttcc-tctggtcacct 72

Query: 1019 acgcccccgttatctccgccgagaaggcctaccacgagcagctgtcggtggctgagatca 1078 | ||||| || |||||||| || |||||||| || || ||| |||| | | | ||||||| Sbjct: 73 atgcccctgtcatctccgcagaaaaggcctatcatgaacagttgtctgcgcc-gagatca 131

Query: 1079 ccaacgcctgcttcgagccggccaaccagatggtcaagtgcgatccccgtcacggcaagt 1138 ||||||||||||||||||| ||||||||||||||||||||||| ||||| |||||||| | Sbjct: 132 ccaacgcctgcttcgagcctgccaaccagatggtcaagtgcgacccccg-cacggcaaat 190

Query: 1139 acatggcctgctgcatgctgtaccgcggtgatgttgtgcccaaggacgtca 1189 |||||||||| |||||| ||||||| || ||||||||||||||||| |||| Sbjct: 191 acatggcctgttgcatgttgtaccgaggagatgttgtgcccaaggatgtca 241 >gb|EE598896.1|EE598896 BT-TOMOV-040-1-F1-T3_F01 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 1267 atcaactaccagccacccaccgtggtgcctggaggtgatttggccaaggtgcagcgtgcc 1326 ||||||||||| |||||||| || || ||||| || ||| | || ||||| || | ||| Sbjct: 18 atcaactaccaaccacccacagttgttcctggtggcgatcttgc-aaggtccaaagagcc 76

Query: 1327 gtgtgcatgttgtccaacaccacggccatcgccgaggcctgggcccgtctggaccacaag 1386 || |||||||||||||||||||| |||||||| || || |||||| |||| ||||||||| Sbjct: 77 gtatgcatgttgtccaacaccaccgccatcgctgaagcttgggcc-gtcttgaccacaag 135

Query: 1387 ttcgatctgatgtacgccaagcgtgccttcgtccactggtacgttggtgagggtatggag 1446 |||||||||||||||||||||||||| |||||||||||||||||| | || |||||||| Sbjct: 136 ttcgatctgatgtacgccaagcgtgctttcgtccactggtacgtt-gagaaggtatggaa 194

Query: 1447 gagggagagttctccgaggcccgtgaggatttggctgccctcgagaaggactacgagg 1504 || ||||| |||||||| ||||||||||| ||||||||||||||| |||||||||||| Sbjct: 195 gaaggagaattctccgaagcccgtgaggacttggctgccctcgag-aggactacgagg 251

Query: 1138 tacatggcctgctgcatgctgtaccgcggtgatgttgtgcccaaggacgtcaacgccgct 1197 ||||||||||| || ||||||||||| || |||||||||||||| |||||||| | | || Sbjct: 4 tacatggcctgttgtatgctgtaccgaggagatgttgtgcccaaagacgtcaatg-ctct 62

Query: 1198 attgccaccatcaagaccaagcgcaccattcaattcgtcgactggtgccccactggcttc 1257 || || |||||||| |||||| | ||||| ||||||||||||||||| |||||||| || Sbjct: 63 atcgcaaccatcaaaaccaagagaaccatccaattcgtcgactggtgtcccactgg-gtc 121

Query: 1258 aaggttggcatcaactaccagccacccaccgtggtgcctggaggtgatttggccaaggtg 1317 |||||||| ||||||||||| ||||||||||| || || || || ||| | |||||||| Sbjct: 122 aaggttggtatcaactaccaaccacccaccgttgttccgggtggagatcttgccaaggt- 180

Query: 1318 cagcgtgccgtgtgcatgttgtccaacaccacggccatcgccgaggcctgggcccgtctg 1377 ||| | |||||||||||||||||||||||||| ||||| || || || ||||||||||| Sbjct: 181 cagagagccgtgtgcatgttgtccaacaccaccgccattgctgaagcttgggcccgtct- 239

Query: 1378 gaccacaagttc 1389 ||||||||||| Sbjct: 240 taccacaagttc 251

>gb|EE598934.1|EE598934 BT-TOMOV-041-1-D9-T3_D09 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 1123 ccccgtcacggcaagtacatggcctgctgcatgctgtaccgcggtgatgttgtgcccaag 1182 |||||||||||||| ||||||||||| |||||| ||||||| || |||||||||||| || Sbjct: 4 ccccgtcacggcaaatacatggcctgttgcatgttgtaccgaggagatgttgtgccc-ag 62

Query: 1183 gacgtcaacgccgctattgccaccatcaagaccaagcgcaccattcaattcgtcgactgg 1242 || ||||| || || || |||||||||||||||||| | ||||| ||||||||||||| | Sbjct: 63 gatgtcaatgctgccatcgccaccatcaagaccaagagaaccatccaattcgtcgact-g 121

Query: 1243 tgccccactggcttcaaggttggcatcaactaccagccacccaccgtggtgcctggaggt 1302 || |||||||| |||||||| || ||||||||||| |||||||| || || ||||| || Sbjct: 122 tgtcccactggtttcaaggtcggtatcaactaccaaccacccacagttgttcctggtgg- 180

Query: 1303 gatttggccaaggtgcagcgtgccgtgtgcatgttgtccaacaccacggccatcgc 1358 ||| | |||||||| || | ||||| |||||||||||||||||||| |||||||| Sbjct: 181 gatcttgccaaggtccaaagagccgtatgcatgttgtccaacaccaccgccatcgc 236

>gb|EE598415.1|EE598415 BT-TOMOV-030-1-D2-T3_D02 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 904 atcgtgtcctcgattaccgcctctctgcgattcgatggtgcccttaacgtggatctgact 963 ||||| ||||| ||||| || ||||| |||||||||||||||||||||| || ||||| Sbjct: 1 atcgtctcctctattacagcttctctcagattcgatggtgcccttaacgttgacttgact 60

Query: 964 gagttccagaccaacttggtgccctacccacgtattcacttccctctggtgacctacgcc 1023 |||||||||| ||| |||| || ||||||||||| |||||||||||||| ||||||| | Sbjct: 61 -agttccagactaacctggtcccatacccacgtatccacttccctctggtcacctacg-c 118

Query: 1024 cccgttatctccgccgagaaggcctaccacgagcagctgtcggtggctgagatcaccaac 1083 || ||||| || || || ||||| ||||| || |||||||| || |||||||||||||| Sbjct: 119 cctgttatttctgctgaaaaggcttaccatgaacagctgtctgttgctgagatcaccaat 178

Query: 1084 gcctgcttcgagccggccaaccagatggtcaagtgcgatccccgtcacggcaagtacatg 1143 | ||| || ||||| |||||||| ||||||||||| || ||||||||||| || |||||| Sbjct: 179 g-ctgttttgagcctgccaaccaaatggtcaagtgtgacccccgtcacggtaaatacatg 237

Query: 1144 gcc 1146 ||| Sbjct: 238 gcc 240

>gb|EE597467.1|EE597467 HBT010_A04_T3_024 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 578 tggccgatcagtgcaccggtctgcagggcttcctcatcttccactcgttcggtggaggta 637 ||||||| || || |||||||| ||||| ||||||||||||| ||| |||||||| |||| Sbjct: 19 tggccgaccaatgtaccggtctccagggtttcctcatcttcc-ctccttcggtggtggta 77

Query: 638 ccggctccggcttcacctcgctgctgatggagcgtctctccgtggactacggcaagaagt 697 |||| ||||| |||||||| || || ||||| |||||||| ||| ||||||| |||||| Sbjct: 78 ccggatccggattcacctcccttctcatggaacgtctctcagtg-actacggaaagaaga 136

Query: 698 ccaagctggagttcgccatctacccagccccccaggtgtccactgccgtggtcgagccct 757 || |||| ||||| |||||||||| || ||||| ||||| | || ||||| |||| Sbjct: 137 gtaaattggaattcgcagtctacccagcacctcaggtatccacc-ctgttgtcgaaccct 195

Query: 758 acaactccatcctgaccacccacaccaccctggagcattccgactgcgccttcatgg 814 ||||||| ||||| ||||||||||||||| |||| || ||||||| ||||||||||| Sbjct: 196 acaactcaatcctcaccacccacaccaccttggaacactccgact-cgccttcatgg 251

Gene 3 Query= gi|24583601|ref|NM_164955.1| Drosophila melanogaster Ribosomal protein L9 CG6141-RB, transcript variant B (RpL9), mRNA (774 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE596378.1|EE596378 BT-H-031-1-G8-T3_G08 Whitefly Bemisia tab... 48 7e- 006 gb|EE602232.1|EE602232 TYLCV008_B12 Whitefly Bemisia tabaci (rea... 48 7e- 006 gb|EE600297.1|EE600297 BT-TYLCV-016-1-A8-T3_A08 Whitefly Bemisia... 32 0.39 gb|EE601209.1|EE601209 BT-TYLCV-036-1-F7-T3_F07 Whitefly Bemisia... 32 0.39 gb|EE602050.1|EE602050 BT-TYLCV-060-1-C1-T3_C01 Whitefly Bemisia... 32 0.39 gb|EE674656.1|EE674656 BT-TYLCV-054-1-C4-T3_C04 Whitefly Bemisia... 32 0.39 gb|EE600814.1|EE600814 BT-TYLCV-027-1-G3-T3_G03 Whitefly Bemisia... 30 1.5 gb|EE601524.1|EE601524 BT-TYLCV-047-1-A12-T3_A12 Whitefly Bemisi... 30 1.5 gb|EE601650.1|EE601650 BT-TYLCV-050-1-C9-T3_C09 Whitefly Bemisia... 30 1.5 gb|EE596066.1|EE596066 BT-H-024-1-H5-T3_H05 Whitefly Bemisia tab... 28 6.1 gb|EE597284.1|EE597284 HBT006_E06_T3_050 Whitefly Bemisia tabaci... 28 6.1 gb|EE597358.1|EE597358 HBT007_H03_T3_031 Whitefly Bemisia tabaci... 28 6.1 gb|EE597523.1|EE597523 HBT011_A11_T3_084 Whitefly Bemisia tabaci... 28 6.1 gb|EE597571.1|EE597571 HBT012_B01 Whitefly Bemisia tabaci (non-v... 28 6.1 gb|EE598802.1|EE598802 BT-TOMOV-038-1-F6-T3_F06 Whitefly Bemisia... 28 6.1 gb|EE598996.1|EE598996 BT-TOMOV-042-1-F2-T3_F02 Whitefly Bemisia... 28 6.1 gb|EE597647.1|EE597647 BT-TOMOV-013-1-H9-T3_H09 Whitefly Bemisia... 28 6.1 gb|EE597801.1|EE597801 BT-TOMOV-017-1-B9-T3_B09 Whitefly Bemisia... 28 6.1 gb|EE598121.1|EE598121 BT-TOMOV-024-1-B3-T3_B03 Whitefly Bemisia... 28 6.1 gb|EE599707.1|EE599707 TOMOV-BT011_D11 Whitefly Bemisia tabaci (... 28 6.1 gb|EE603861.1|EE603861 BT-HINST-024-1-G4-T3_G04 Whitefly Bemisia... 28 6.1 gb|EE604439.1|EE604439 BT-HINST-046-1-B12-T3_B12 Whitefly Bemisi... 28 6.1 gb|EE602370.1|EE602370 TYLCV010_G06 Whitefly Bemisia tabaci (rea... 28 6.1 gb|EE600605.1|EE600605 BT-TYLCV-023-1-B1-T3_B01 Whitefly Bemisia... 28 6.1 gb|EE601109.1|EE601109 BT-TYLCV-034-1-D3-T3_D03 Whitefly Bemisia... 28 6.1 gb|EE601996.1|EE601996 BT-TYLCV-059-1-B12-T3_B12 Whitefly Bemisi... 28 6.1

>gb|EE596378.1|EE596378 BT-H-031-1-G8-T3_G08 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Score = 48.1 bits (24), Expect = 7e-006 Identities = 24/24 (100%) Strand = Plus / Plus

Query: 407 tacgcccatttccccatcaactgt 430 |||||||||||||||||||||||| Sbjct: 213 tacgcccatttccccatcaactgt 236 >gb|EE602232.1|EE602232 TYLCV008_B12 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 494

Query: 407 tacgcccatttccccatcaactgt 430 |||||||||||||||||||||||| Sbjct: 155 tacgcccatttccccatcaactgt 178

Score = 34.2 bits (17), Expect = 0.098 Identities = 17/17 (100%) Strand = Plus / Plus

Query: 629 aaggatatccgtaagtt 645 ||||||||||||||||| Sbjct: 373 aaggatatccgtaagtt 389

Score = 32.2 bits (16), Expect = 0.39 Identities = 19/20 (95%) Strand = Plus / Minus

Query: 15 tatatttttattgaaatttt 34 |||||||||||| ||||||| Sbjct: 637 tatatttttattcaaatttt 618

Gene 4

Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402.

Query= gi|19527546|gb|AY084150.1| Drosophila melanogaster RE30552 full insert cDNA (2544 letters)

Searching...... done

Score E Sequences producing significant alignments: (bits) Value gb|EE602484.1|EE602484 TYLCV-BT012_E06 Whitefly Bemisia tabaci (... 113 4e- 025 gb|EE596178.1|EE596178 BT-H-027-1-C2-T3_C02 Whitefly Bemisia tab... 34 0.33 gb|EE602299.1|EE602299 TYLCV009_D09 Whitefly Bemisia tabaci (rea... 34 0.33 gb|EE598187.1|EE598187 BT-TOMOV-025-1-E6-T3_E06 Whitefly Bemisia... 32 1.3 gb|EE598456.1|EE598456 BT-TOMOV-031-1-B1-T3_B01 Whitefly Bemisia... 32 1.3 gb|EE596879.1|EE596879 BT-H-044-1-B4-T3_B04 Whitefly Bemisia tab... 30 5.1 gb|EE598253.1|EE598253 BT-TOMOV-027-1-A5-T3_A05 Whitefly Bemisia... 30 5.1 gb|EE602691.1|EE602691 BT-HEG-007-1-H12-T3_H12 Whitefly Bemisia ... 30 5.1 gb|EE600337.1|EE600337 BT-TYLCV-017-1-A10-T3_A10 Whitefly Bemisi... 30 5.1 gb|EE600346.1|EE600346 BT-TYLCV-017-1-B9-T3_B09 Whitefly Bemisia... 30 5.1 gb|EE600362.1|EE600362 BT-TYLCV-017-1-F11-T3_F11 Whitefly Bemisi... 30 5.1 gb|EE600374.1|EE600374 BT-TYLCV-017-1-H11-T3_H11 Whitefly Bemisi... 30 5.1 gb|EE600375.1|EE600375 BT-TYLCV-017-1-H12-T3_H12 Whitefly Bemisi... 30 5.1 gb|EE600880.1|EE600880 BT-TYLCV-029-1-A8-T3_A08 Whitefly Bemisia... 30 5.1 gb|EE602335.1|EE602335 TYLCV010_A10 Whitefly Bemisia tabaci (rea... 30 5.1 gb|EE600340.1|EE600340 BT-TYLCV-017-1-A9-T3_A09 Whitefly Bemisia... 30 5.1 gb|EE600341.1|EE600341 BT-TYLCV-017-1-B10-T3_B10 Whitefly Bemisi... 30 5.1 gb|EE601487.1|EE601487 BT-TYLCV-046-1-C10-T3_C10 Whitefly Bemisi... 30 5.1

>gb|EE602484.1|EE602484 TYLCV-BT012_E06 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 359

Query: 1049 atcatgaagcgtaccgcccttgtggccaacacctccaacatgcctgtggctgctcgtgag 1108 |||||||| ||||| || || || ||||||||||||| ||||||||| |||||||| || Sbjct: 24 atcatgaaacgtacagcactggtagccaacacctcca-catgcctgtcgctgctcgagaa 82

Query: 1109 gcctccatctacactggtatcaccttgtccgaatacttccgtgatatgggttacaacgtg 1168 |||||||||||||| |||||||| | || || ||||| || || ||||| |||||||| Sbjct: 83 gcctccatctacacaggtatcacactatctgagtactt-cgagacatgggctacaacgtt 141

Query: 1169 tccatgatggctgattccacctcccgttgggctgaggctcttcgtgaaatttctggtcgt 1228 |||||||||||||| || ||||| ||||||||||| || || | |||||||| |||||| Sbjct: 142 tccatgatggctgactctacctctcgttgggctgaagc-ctgagagaaatttcgggtcgt 200

Query: 1229 ctcgctgagatgcctgc 1245 | ||||| |||||||| Sbjct: 201 ttggctgaaatgcctgc 217

Query: 1352 attgtcggagctgtgtctcctcctggtggtgacttctc 1389 |||||||| || || |||||||||||||| |||||||| Sbjct: 322 attgtcggtgccgtatctcctcctggtggagacttctc 359

Query: 3 tgagcttttcgaagtgcgagc 23 ||||||| ||||||||||||| Sbjct: 136 tgagcttctcgaagtgcgagc 116

>gb|EE602299.1|EE602299 TYLCV009_D09 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 394

Query: 1439 ctcgacaagaagttggcccag 1459 |||||||||||| |||||||| Sbjct: 366 ctcgacaagaagatggcccag 386

>gb|EE598187.1|EE598187 BT-TOMOV-025-1-E6-T3_E06 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 2414 ttatttgtgaaaaaag 2429 |||||||||||||||| Sbjct: 62 ttatttgtgaaaaaag 47

>gb|EE598456.1|EE598456 BT-TOMOV-031-1-B1-T3_B01 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 2413 tttatttgtgaaaaaa 2428 |||||||||||||||| Sbjct: 160 tttatttgtgaaaaaa 175

Gene 5

Query= gi|24641091|ref|NM_167247.1| Drosophila melanogaster stress-sensitive B CG16944-RD, transcript variant D (sesB), mRNA (1756 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE602172.1|EE602172 TYLCV006_A02 Whitefly Bemisia tabaci (rea... 117 2e- 026 gb|EE600486.1|EE600486 BT-TYLCV-020-1-C3-T3_C03 Whitefly Bemisia... 113 3e- 025 gb|EE596940.1|EE596940 BT-H-045-1-F11-T3_F11 Whitefly Bemisia ta... 105 7e- 023 gb|EE596212.1|EE596212 BT-H-028-1-C2-T3_C02 Whitefly Bemisia tab... 103 3e- 022 gb|EE602286.1|EE602286 TYLCV009_C03 Whitefly Bemisia tabaci (rea... 103 3e- 022 gb|EE600330.1|EE600330 BT-TYLCV-016-1-G6-T3_G06 Whitefly Bemisia... 103 3e- 022 gb|EE600537.1|EE600537 BT-TYLCV-021-1-C1-T3_C01 Whitefly Bemisia... 103 3e- 022 gb|EE600871.1|EE600871 BT-TYLCV-028-1-G6-T3_G06 Whitefly Bemisia... 103 3e- 022 gb|EE601529.1|EE601529 BT-TYLCV-047-1-B12-T3_B12 Whitefly Bemisi... 103 3e- 022 gb|EE600328.1|EE600328 BT-TYLCV-016-1-G4-T3_G04 Whitefly Bemisia... 101 1e- 021 gb|EE598307.1|EE598307 BT-TOMOV-028-1-A9-T3_A09 Whitefly Bemisia... 86 7e- 017 gb|EE601623.1|EE601623 BT-TYLCV-049-1-F9-T3_F09 Whitefly Bemisia... 80 4e- 015 gb|EE601734.1|EE601734 BT-TYLCV-052-1-D7-T3_D07 Whitefly Bemisia... 80 4e- 015 gb|EE601901.1|EE601901 BT-TYLCV-056-1-H9-T3_H09 Whitefly Bemisia... 80 4e- 015 gb|EE674634.1|EE674634 BT-TYLCV-041-1-D5-T3_D05 Whitefly Bemisia... 80 4e- 015 gb|EE599263.1|EE599263 TOMOV-BT003_G09 Whitefly Bemisia tabaci (... 72 1e- 012 gb|EE597576.1|EE597576 HBT012_B09 Whitefly Bemisia tabaci (non-v... 64 3e- 010 gb|EE597092.1|EE597092 HBT002_E04_T3_034 Whitefly Bemisia tabaci... 64 3e- 010 gb|EE599259.1|EE599259 TOMOV-BT003_G02 Whitefly Bemisia tabaci (... 64 3e- 010 gb|EE596344.1|EE596344 BT-H-031-1-A5-T3_A05 Whitefly Bemisia tab... 58 2e- 008 gb|EE596448.1|EE596448 BT-H-034-1-C9-T3_C09 Whitefly Bemisia tab... 58 2e- 008 gb|EE597748.1|EE597748 BT-TOMOV-016-1-B7-T3_B07 Whitefly Bemisia... 58 2e- 008 gb|EE597582.1|EE597582 HBT012_D02 Whitefly Bemisia tabaci (non-v... 52 1e- 006 gb|EE598016.1|EE598016 BT-TOMOV-021-1-H8-T3_H08 Whitefly Bemisia... 52 1e- 006 gb|EE597639.1|EE597639 BT-TOMOV-013-1-G11-T3_G11 Whitefly Bemisi... 48 2e- 005 gb|EE597964.1|EE597964 BT-TOMOV-020-1-H10-T3_H10 Whitefly Bemisi... 46 6e- 005 gb|EE598383.1|EE598383 BT-TOMOV-029-1-G12-T3_G12 Whitefly Bemisi... 46 6e- 005 gb|EE599995.1|EE599995 BT_TYLCV002_F11 Whitefly Bemisia tabaci (... 46 6e- 005 gb|EE600129.1|EE600129 BT_TYLCV005_C10 Whitefly Bemisia tabaci (... 46 6e- 005 gb|EE601183.1|EE601183 BT-TYLCV-036-1-B2-T3_B02 Whitefly Bemisia... 46 6e- 005 gb|EE601865.1|EE601865 BT-TYLCV-056-1-A8-T3_A08 Whitefly Bemisia... 46 6e- 005 gb|EE600538.1|EE600538 BT-TYLCV-021-1-C2-T3_C02 Whitefly Bemisia... 44 2e- 004 gb|EE600209.1|EE600209 BT-TYLCV-014-1-B3-T3_B03 Whitefly Bemisia... 36 0.057 gb|EE599223.1|EE599223 TOMOV-BT003_B07 Whitefly Bemisia tabaci (... 34 0.23 gb|EE601949.1|EE601949 BT-TYLCV-058-1-B3-T3_B03 Whitefly Bemisia... 34 0.23 gb|EE596099.1|EE596099 BT-H-025-1-F3-T3_F03 Whitefly Bemisia tab... 32 0.89 gb|EE596261.1|EE596261 BT-H-029-1-D5-T3_D05 Whitefly Bemisia tab... 32 0.89 gb|EE604524.1|EE604524 BT-HINST-048-1-A1-T3_F04 Whitefly Bemisia... 32 0.89 gb|EE601803.1|EE601803 BT-TYLCV-053-1-H12-T3_H12 Whitefly Bemisi... 32 0.89 gb|EE599967.1|EE599967 BT_TYLCV002_B06 Whitefly Bemisia tabaci (... 32 0.89 gb|EE601615.1|EE601615 BT-TYLCV-049-1-D3-T3_D03 Whitefly Bemisia... 32 0.89 gb|EE595576.1|EE595576 BT-H-014-1-D5-T3_D05 Whitefly Bemisia tab... 30 3.5 gb|EE596209.1|EE596209 BT-H-028-1-B9-T3_B09 Whitefly Bemisia tab... 30 3.5 gb|EE603459.1|EE603459 BT-HINST-016-1-F11-T3_F11 Whitefly Bemisi... 30 3.5 gb|EE601234.1|EE601234 BT-TYLCV-037-1-C11-T3_C11 Whitefly Bemisi... 30 3.5 gb|EE601482.1|EE601482 BT-TYLCV-046-1-B1-T3_B01 Whitefly Bemisia... 30 3.5 gb|EE602181.1|EE602181 TYLCV006_B07 Whitefly Bemisia tabaci (rea... 30 3.5 gb|EE600286.1|EE600286 BT-TYLCV-015-1-H10-T3_H10 Whitefly Bemisi... 30 3.5 gb|EE674608.1|EE674608 BT-TYLCV-034-1-E4-T3_E04 Whitefly Bemisia... 30 3.5

>gb|EE602172.1|EE602172 TYLCV006_A02 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 441

Query: 435 ttctggcgcggcaacttggccaacgtcatcagatacttcccaacccaggctctgaacttt 494 |||||||| || ||| | ||||| |||||| | |||||||| || |||||| ||||||| Sbjct: 71 ttctggcgtggtaacctcgccaatgtcatccgttacttccccacacaggct-tgaacttc 129

Query: 495 gccttcaaggacaagtacaagcaggtcttcctgggtggcgtggacaagaacacccagttc 554 |||||||||||||||||||||||||||||| |||||||||| ||||||||| | || ||| Sbjct: 130 gccttcaaggacaagtacaagcaggtcttcttgggtggcgttgacaagaac-ctcaattc 188

Query: 555 tggcgctacttcgccggcaacttggcctccggtggtgccgctggtgc 601 | | || ||||| || ||||||||||| |||||||| |||||||| Sbjct: 189 atgaggtatttcgctggtaacttggcctctggtggtgctgctggtgc 235

Query: 681 gaattcaccggtctgggcaactgcttgaccaagatcttcaagagcgacggcatcgttgga 740 ||||||| |||||| || |||| ||| ||||||||||||| | | |||| ||||||||| Sbjct: 316 gaattcaacggtcttggagactgtttgtccaagatcttcaa-atctacggtatcgttgga 374

Query: 741 ttgtaccgtggtttcggagtgtccgtgcagggcatcatcatctac 785 |||||| | ||||||||||| || || || || |||||| ||||| Sbjct: 375 ttgtacagaggtttcggagtctcagtccaaggtatcatcttctac 419

Query: 435 ttctggcgcggcaacttggccaacgtcatcagatacttcccaacccaggctctgaacttt 494 |||||||| || ||| | ||||| |||||| | |||||||| || ||||| |||||| Sbjct: 63 ttctggcgtggtaacctcgccaatgtcatccgttacttccccacacaggccttgaactc- 121

Query: 495 gccttcaaggacaagtacaagcaggtcttcctgggtggcgtggacaagaacacccagttc 554 |||||||||||||||||||||||||||||| |||||||||| ||||||||||| || || Sbjct: 122 gccttcaaggacaagtacaagcaggtcttcttgggtggcgttgacaagaacactcaatta 181

Query: 555 tggcgctacttcgccggcaacttggcctccggtggtgccgctggtgctacctctctgtgc 614 | | | || ||||| || ||||||||||| |||||||| |||||||| || ||||| ||| Sbjct: 182 t-gaggtatttcgctggtaacttggcctctggtggtgctgctggtgccacttctctctgc 240

Query: 615 t 615 | Sbjct: 241 t 241

Query: 681 gaattcaccggtctgggcaactgcttgaccaagatcttcaagagcgacggcatcgttgga 740 ||||||| |||||| || |||| ||| ||||||||||||| ||||| || |||||| Sbjct: 308 gaattcaacggtcttggagactgtttgtccaagatcttcaaatctgacggtat-gttgga 366

Query: 741 ttgtaccgtggtttcggagtgtccgtgcagggcatcatcatctac 785 |||||| | ||||||||||| || || || || |||||||||||| Sbjct: 367 ttgtacagaggtttcggagtctcagtccaaggtatcatcatctac 411

Score = 42.1 bits (21), Expect = 0.001 Identities = 64/77 (83%), Gaps = 1/77 (1%) Strand = Plus / Plus

Query: 979 agaacacactgcactgctgggccaccatcgccaagcaggagggcaccggtgccttcttca 1038 |||||||||| ||||||||||| ||| |||||| || ||||| || ||||||||| Sbjct: 601 agaacacactccactgctgggctgtcattgccaagactgaaggcacaggcgccttcttc- 659

Query: 1039 agggcgccttctccaac 1055 |||| |||||||||||| Sbjct: 660 agggagccttctccaac 676

Query: 936 atgatgatgcagtctggtcg 955 |||||||||||||||||||| Sbjct: 559 atgatgatgcagtctggtcg 578

>gb|EE596940.1|EE596940 BT-H-045-1-F11-T3_F11 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 495 gccttcaaggacaagtacaagcaggtcttcctgggtggcgtggacaagaacacccagttc 554 |||||||||||||||||||||||||||||| |||||||||| ||||||||||| || || Sbjct: 2 gccttcaaggacaagtacaagcaggtcttcttgggtggcgttgacaagaacactcaatta 61

Query: 555 tggcgctacttcgccggcaacttggcctccggtggtgccgctggtgctacctctctgtgc 614 | | | || ||||| || ||||||||||| |||||||| |||||||| || ||||| ||| Sbjct: 62 t-gaggtatttcgctggtaacttggcctctggtggtgctgctggtgccacttctctctgc 120

Query: 615 t 615 | Sbjct: 121 t 121

Query: 681 gaattcaccggtctgggcaactgcttgaccaagatcttcaa 721 ||||||| |||||| || |||| ||| ||||||||||||| Sbjct: 188 gaattcaacggtcttggagactgtttgtccaagatcttcaa 228

Query: 453 gccaacgtcatcagatacttcccaacccaggctctgaactttgccttcaaggacaagtac 512 ||||| |||||| | |||||||| || ||||| ||||||| ||||||||||||||||| Sbjct: 2 gccaatgtcatccgttacttccccacacaggccttgaacttcgccttcaaggacaagta- 60

Query: 513 aagcaggtcttcctgggtggcgtggacaagaacacccagttctggcgctacttcgccggc 572 |||||||||||| |||||||||| ||||||||||| || ||| | | || ||||| || Sbjct: 61 aagcaggtcttcttgggtggcgttgacaagaacactcaattcatgaggtatttcgctgg- 119

Query: 573 aacttggcctccggtggtgccgctggtgctacctctctgtgctt 616 |||||||||| |||||||| |||||||| || ||||| ||||| Sbjct: 120 tacttggcctctggtggtgctgctggtgccacttctctctgctt 163

>gb|EE602286.1|EE602286 TYLCV009_C03 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 686

Query: 453 gccaacgtcatcagatacttcccaacccaggctctgaactttgccttcaaggacaagtac 512 ||||| |||||| | |||||||| || ||||| ||||||| |||||||||||||| ||| Sbjct: 305 gccaatgtcatccgttacttccccacacaggccttgaacttcgccttcaaggacaa-tac 363

Query: 513 aagcaggtcttcctgggtggcgtggacaagaacacccagttctggcgctacttcgccggc 572 |||||||||||| |||||||||| ||||||||||| || ||| | | || |||| | | Sbjct: 364 aagcaggtcttcttgggtggcgttgacaagaacactcaattcatgaggtatttcg-ctgt 422

Query: 573 aacttggcctccggtggtgccgctggtgctacctctctgtgctt 616 ||||||||||| |||||||| |||||||| || ||||| ||||| Sbjct: 423 aacttggcctctggtggtgctgctggtgccacttctctctgctt 466

Query: 700 actgcttgaccaagatcttcaagagcgacggcatcgttggattgtaccgtggtttcggag 759 |||| ||| ||||||||||||| ||||| ||||||||||||||| | ||||||||| Sbjct: 551 actgtttgtccaagatcttcaaatctgacggtatcgttggattgtacag-agtttcggag 609

Query: 760 tgtccgtgcagggcatcatcatctaccgtgccgcctacttcgg 802 | || || || || |||||||||||| | ||| | |||||||| Sbjct: 610 tctcagtccaaggtatcatcatctacagagcctcttacttcgg 652

Query: 267 gccggaggcatctccgcagctgtctccaagactgccgtcgcccccattgagcgtgt 322 |||||||| ||||| || || || ||||| || |||||||||||||| || ||||| Sbjct: 122 gccggaggtatctctgccgccgtttccaaaaccgccgtcgcccccatcgaacgtgt 177

Query: 453 gccaacgtcatcagatacttcccaacccaggctctgaactttgccttcaaggacaagtac 512 ||||| |||||| | |||||||| || ||||| ||||||| ||||||||||||||| || Sbjct: 304 gccaatgtcatccgttacttccccacacaggccttgaacttcgccttcaaggacaag-ac 362

Query: 265 ctgccggaggcatctccgcagctgtctccaagactgccgtcgcccccattgagcgtgt 322 |||||||||| ||||| || || || ||||| || |||||||||||||| || ||||| Sbjct: 119 ctgccggaggtatctctgccgccgtttccaaaaccgccgtcgcccccatcgaacgtgt 176

Query: 760 tgtccgtgcagggcatcatcatctac 785 | || || || || |||||||||||| Sbjct: 609 tctcagtccaaggtatcatcatctac 634

Query: 936 atgatgatgcagtctggtc 954 ||||||||||||||||||| Sbjct: 722 atgatgatgcagtctggtc 740

Query: 453 gccaacgtcatcagatacttcccaacccaggctctgaactttgccttcaaggacaagtac 512 ||||| |||||| | |||||||| || ||||| ||||||| |||||||||||||| ||| Sbjct: 305 gccaatgtcatccgttacttccccacacaggccttgaacttcgccttcaaggacaa-tac 363

Query: 267 gccggaggcatctccgcagctgtctccaagactgccgtcgcccccattgagcgtgt 322 |||||||| ||||| || || || ||||| || |||||||||||||| || ||||| Sbjct: 122 gccggaggtatctctgccgccgtttccaaaaccgccgtcgcccccatcgaacgtgt 177

>gb|EE601529.1|EE601529 BT-TYLCV-047-1-B12-T3_B12 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 686

Query: 487 tgaactttgccttcaaggacaagtacaagcaggtcttcctgggtggcgtggacaagaaca 546 ||||||| |||||||||||||||||||||||||||||| |||||||||| |||||||||| Sbjct: 1 tgaacttcgccttcaaggacaagtacaagcaggtcttcttgggtggcgttgacaagaaca 60

Query: 547 cccagttctggcgctacttcgccggcaacttggcctccggtggtgccgctggtgc 601 || ||| | | || ||||| || ||||||||||| |||||||| |||||||| Sbjct: 61 -tcaattcatgaggtatttcgctggtaacttggcctctggtggtgctgctggtgc 114

Query: 972 atctacaagaacacactgcactgctgggccaccatcgccaagcaggagggcaccggtgcc 1031 ||||||||||||||||| ||||||||||| ||| |||||| || ||||| || ||| Sbjct: 481 atctacaagaacacactccactgctgggctgtcattgccaagactgaaggcacaggcgcc 540

Query: 1032 ttcttcaagggcgccttctccaacattctcagaggaactggtggcgctttcgtgcttgt 1090 |||||||||| |||||||||||| | | |||||||||||||||||| |||| ||||| Sbjct: 541 -tcttcaagggagccttctccaacgtattgagaggaactggtggcgctctcgtccttgt 598 Score = 58.0 bits (29), Expect = 2e-008 Identities = 87/105 (82%), Gaps = 1/105 (0%) Strand = Plus / Plus

Query: 681 gaattcaccggtctgggcaactgcttgaccaagatcttcaagagcgacggcatcgttgga 740 ||||||| |||||| || |||| ||| ||||||||||||| | | ||| ||||||||| Sbjct: 195 gaattcaacggtcttggagactgtttgtccaagatcttcaa-atctgcggtatcgttgga 253

Query: 741 ttgtaccgtggtttcggagtgtccgtgcagggcatcatcatctac 785 |||||| | ||||||||||| || || || || |||||||||||| Sbjct: 254 ttgtacagaggtttcggagtctcagtccaaggtatcatcatctac 298

>gb|EE598307.1|EE598307 BT-TOMOV-028-1-A9-T3_A09 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 487 tgaactttgccttcaaggacaagtacaagcaggtcttcctgggtggcgtggacaa 541 ||||||| |||||||||||||||||||||||||||||| |||||||||| ||||| Sbjct: 186 tgaacttcgccttcaaggacaagtacaagcaggtcttcttgggtggcgttgacaa 240

Query: 972 atctacaagaacacactgcactgctgggccaccatcgccaagcaggagggcaccggtgcc 1031 ||||||||||||||||| |||||||||| | || |||||| || ||||| || ||| Sbjct: 393 atctacaagaacacactccactgctgggtgtc-attgccaagactgaaggcacaggcgcc 451

Query: 1032 ttcttcaagggcgccttctccaacattctcagaggaactggtggcgctttcgtgcttgtg 1091 ||||||||||| |||||||||||| | | |||||||||||||||||| |||| ||||| Sbjct: 452 ttcttcaagggagccttctccaacgtatt-agaggaactggtggcgctctcgtccttgtc 510

Query: 1092 ttgtacgatgagatcaagaa 1111 || ||||| ||| ||||||| Sbjct: 511 ttctacgacgagctcaagaa 530

Query: 681 gaattcaccggtctgggcaactgcttgaccaagatcttcaagagcgacggcatcgttgga 740 ||||||| |||||| || ||||| ||| ||||||||||||| ||||| ||||||||| Sbjct: 103 gaattcaacggtcttgg-aactgtttgtccaagatcttcaaatctgacggtatcgttgga 161

Query: 741 ttgtaccgtggtttcggag 759 |||||| | |||||||||| Sbjct: 162 ttgtacagaggtttcggag 180

Query: 972 atctacaagaacacactgcactgctgggccaccatcgccaagcaggagggcaccggtgcc 1031 ||||||||||||||||| ||||||||||| ||| |||||| |||| ||| || ||| Sbjct: 435 atctacaagaacacactccactgctgggctgtcattgccaagactgagg-cacaggcgcc 493

Query: 1032 ttcttcaagggcgccttctccaacattctcagaggaactggtggcgctttcgtgcttgtg 1091 ||||||||||| |||||||||||| | | ||||||||||||||||| |||| ||||| Sbjct: 494 ttcttcaagggagccttctccaacgtattgagaggaactggtggcgc-ctcgtccttgtc 552

Query: 726 gacggcatcgttggattgtaccgtggtttcggagt 760 ||||| ||||||||||||||| | ||||||||||| Sbjct: 193 gacggtatcgttggattgtacagaggtttcggagt 227

Query: 564 ttcgccggcaacttggcctccggtggtgc 592 ||||| || ||||||||||| |||||||| Sbjct: 31 ttcgctggtaacttggcctctggtggtgc 59

Query: 972 atctacaagaacacactgcactgctgggccaccatcgccaagcaggagggcaccggtgcc 1031 ||||||||||||||||| ||||||||||| ||| |||||| || ||||| || ||| Sbjct: 26 atctacaagaacacactccactgctgggctgtcat-gccaagactgaaggcacaggcgcc 84

Query: 1032 ttcttcaagggcgccttctccaacattctcagaggaactggtggcgctttcgtgcttgtg 1091 ||||||||||| |||||||||||| | | ||||| |||||||||||| |||| ||||| Sbjct: 85 ttcttcaagggagccttctccaacgtattgagagg-actggtggcgctctcgtccttgtc 143

Query: 1092 ttgtacgatgagatcaagaa 1111 || ||||| ||| ||||||| Sbjct: 144 ttctacgacgagctcaagaa 163 >gb|EE674634.1|EE674634 BT-TYLCV-041-1-D5-T3_D05 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 470

Query: 972 atctacaagaacacactgcactgctgggccaccatcgccaagcaggagggcaccggtgcc 1031 ||||||||||||||||| ||||||||||| ||| |||||| || ||||| || || Sbjct: 243 atctacaagaacacactccactgctgggctgtcattgccaagactgaaggcacaggcgc- 301

Query: 1032 ttcttcaagggcgccttctccaacattctcagaggaactggtggcgctttcgtgcttgtg 1091 ||||||||||| |||||||||||| | | |||||||||||||||||| |||| ||||| Sbjct: 302 ttcttcaagggagccttctccaacgtattgagaggaactggtggcgctctcgtccttgt- 360

Query: 726 gacggcatcgttggattgtaccgtggtttcggagtgtccgtgcagggcatcatcatctac 785 ||||| ||||||||||||||| | ||||||||||| || || || || |||||||||||| Sbjct: 1 gacggtatcgttggattgtacagaggtttcggagtctcagtccaaggtatcatcatctac 60

>gb|EE599263.1|EE599263 TOMOV-BT003_G09 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 972 atctacaagaacacactgcactgctgggccaccatcgccaagcaggagggcaccggtgcc 1031 ||||||||||||||||| | ||||||||| ||| |||||| || ||||| || ||| Sbjct: 42 atctacaagaacacactcc-ctgctgggctgtcattgccaagactgaaggcacaggcgcc 100

Query: 1032 ttcttcaagggcgccttctccaacattctcagaggaactggtggcgctttcgtgcttgtg 1091 ||||||||||| |||||||| ||| | | |||||||||||||||||| |||| ||||| Sbjct: 101 ttcttcaagggagccttctc-aacgtattgagaggaactggtggcgctctcgtccttgtc 159

>gb|EE597576.1|EE597576 HBT012_B09 Whitefly Bemisia tabaci (non-viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 1020 ggcaccggtgccttcttcaagggcgccttctccaacattctcagaggaactggtggcgct 1079 ||||| || |||||||||||||| |||||||||||| | | ||||||||||||||||| Sbjct: 19 ggcacaggcgccttcttcaagggagccttctccaacgtattg-gaggaactggtggcgct 77

Query: 1080 ttcgtgcttgtgttgtacgatgagatcaagaa 1111 |||| ||||| || ||||| ||| ||||||| Sbjct: 78 ctcgtccttgtcttctacgacgagctcaagaa 109

>gb|EE597092.1|EE597092 HBT002_E04_T3_034 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Gene 6

Query= gi|24643008|ref|NM_078676.3| Drosophila melanogaster beta-coatomer protein CG6223-RA (betaCop), mRNA (3158 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE601196.1|EE601196 BT-TYLCV-036-1-D5-T3_D05 Whitefly Bemisia... 36 0.10 gb|EE601903.1|EE601903 BT-TYLCV-057-1-A11-T3_A11 Whitefly Bemisi... 34 0.41 gb|EE600771.1|EE600771 BT-TYLCV-026-1-G11-T3_G11 Whitefly Bemisi... 32 1.6 gb|EE600772.1|EE600772 BT-TYLCV-026-1-G12-T3_G12 Whitefly Bemisi... 32 1.6 gb|EE600309.1|EE600309 BT-TYLCV-016-1-D2-T3_D02 Whitefly Bemisia... 30 6.3 gb|EE600954.1|EE600954 BT-TYLCV-030-1-F1-T3_F01 Whitefly Bemisia... 30 6.3 gb|EE601203.1|EE601203 BT-TYLCV-036-1-E8-T3_E08 Whitefly Bemisia... 30 6.3 gb|EE600522.1|EE600522 BT-TYLCV-020-1-H7-T3_H07 Whitefly Bemisia... 30 6.3 gb|EE601100.1|EE601100 BT-TYLCV-034-1-B10-T3_B10 Whitefly Bemisi... 30 6.3 gb|EE600680.2|EE600680 BT-TYLCV-042-1-F5-T3_F05 Whitefly Bemisia... 30 6.3

Query: 2976 gcagtcggtgcaggcggc 2993 |||||||||||||||||| Sbjct: 205 gcagtcggtgcaggcggc 188

Query: 1781 acggcgacttcttcatc 1797 ||||||||||||||||| Sbjct: 631 acggcgacttcttcatc 647

Gene 7

Query= gi|24663671|ref|NM_079329.2| Drosophila melanogaster Ribosomal protein S4 CG11276-RB, transcript variant B (RpS4), mRNA (969 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE601963.1|EE601963 BT-TYLCV-058-1-D11-T3_D11 Whitefly Bemisi... 38 0.008 gb|EE597044.1|EE597044 HBT001_F06_T3_058 Whitefly Bemisia tabaci... 36 0.031 gb|EE597460.1|EE597460 HBT009_G12_T3_099 Whitefly Bemisia tabaci... 34 0.12 gb|EE599345.1|EE599345 TOMOV-BT005_B09 Whitefly Bemisia tabaci (... 34 0.12 gb|EE599718.1|EE599718 TOMOV-BT011_F05 Whitefly Bemisia tabaci (... 34 0.12 gb|EE597618.1|EE597618 BT-TOMOV-013-1-B11-T3_B11 Whitefly Bemisi... 34 0.12 gb|EE603198.1|EE603198 BT-HINST-010-1-F8-T3_F08 Whitefly Bemisia... 34 0.12 gb|EE601538.1|EE601538 BT-TYLCV-047-1-D9-T3_D09 Whitefly Bemisia... 34 0.12 gb|EE600132.1|EE600132 BT_TYLCV005_D03 Whitefly Bemisia tabaci (... 34 0.12 gb|EE596599.1|EE596599 BT-H-037-1-E7-T3_E07 Whitefly Bemisia tab... 32 0.49 gb|EE597528.1|EE597528 HBT011_B05_T3_044 Whitefly Bemisia tabaci... 32 0.49 gb|EE600954.1|EE600954 BT-TYLCV-030-1-F1-T3_F01 Whitefly Bemisia... 32 0.49 gb|EE597423.1|EE597423 HBT009_A12_T3_088 Whitefly Bemisia tabaci... 30 1.9 gb|EE601442.1|EE601442 BT-TYLCV-045-1-C6-T3_C06 Whitefly Bemisia... 30 1.9 gb|EE602507.1|EE602507 TYLCV-BT012_H02 Whitefly Bemisia tabaci (... 30 1.9 gb|EE602570.1|EE602570 BT-HEG-002-1-E6-T3_E06 Whitefly Bemisia t... 28 7.6 gb|EE600151.1|EE600151 BT_TYLCV005_G06 Whitefly Bemisia tabaci (... 28 7.6 gb|EE600828.1|EE600828 BT-TYLCV-028-1-A3-T3_A03 Whitefly Bemisia... 28 7.6 gb|EE601650.1|EE601650 BT-TYLCV-050-1-C9-T3_C09 Whitefly Bemisia... 28 7.6 gb|EE600303.1|EE600303 BT-TYLCV-016-1-C1-T3_C01 Whitefly Bemisia... 28 7.6 gb|EE601759.1|EE601759 BT-TYLCV-052-1-H9-T3_H09 Whitefly Bemisia... 28 7.6

Query: 516 gttacacacgacggtcgcaccatccgctacccggatccc 554 ||||||||||| || |||||||| ||||| || |||||| Sbjct: 203 gttacacacgatgggcgcaccattcgctatcctgatccc 241

Query: 356 catggatgtcatca 369 |||||||||||||| Sbjct: 45 catggatgtcatca 58

>gb|EE597044.1|EE597044 HBT001_F06_T3_058 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 516 gttacacacgacggtcgcaccatccgctacccggatcc 553 ||||||||||| || |||||||| ||||| || ||||| Sbjct: 76 gttacacacgatgggcgcaccattcgctatcctgatcc 113

Gene 8 Query= gi|24580840|ref|NM_134750.1| Drosophila melanogaster CG5440 CG5440-RA (CG5440), mRNA (495 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE597332.1|EE597332 HBT007_D04_T3_041 Whitefly Bemisia tabaci... 28 3.9 gb|EE599123.1|EE599123 BT-TOMOV-045-1-B8-T3_B08 Whitefly Bemisia... 28 3.9 gb|EE597776.1|EE597776 BT-TOMOV-016-1-G12-T3_G12 Whitefly Bemisi... 28 3.9 gb|EE601218.1|EE601218 BT-TYLCV-036-1-G9-T3_G09 Whitefly Bemisia... 28 3.9 gb|EE601443.1|EE601443 BT-TYLCV-045-1-C9-T3_C09 Whitefly Bemisia... 28 3.9 gb|EE600260.1|EE600260 BT-TYLCV-015-1-C3-T3_C03 Whitefly Bemisia... 28 3.9 gb|EE600803.1|EE600803 BT-TYLCV-027-1-E2-T3_E02 Whitefly Bemisia... 28 3.9 gb|EE601501.1|EE601501 BT-TYLCV-046-1-E1-T3_E01 Whitefly Bemisia... 28 3.9

>gb|EE597332.1|EE597332 HBT007_D04_T3_041 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 13 ttttctgccgagga 26 |||||||||||||| Sbjct: 201 ttttctgccgagga 214

>gb|EE599123.1|EE599123 BT-TOMOV-045-1-B8-T3_B08 Whitefly Bemisia tabaci (reared on TOMOV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 169 atcatcggacccgcggac 186 |||||||||||| ||||| Sbjct: 155 atcatcggacccccggac 172 Gene 9

Query= gi|85724999|ref|NM_001038847.1| Drosophila melanogaster Glyceraldehyde 3 phosphate dehydrogenase 1 CG12055-RB, transcript variant B (Gapdh1), mRNA (1285 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE601511.1|EE601511 BT-TYLCV-046-1-G1-T3_G01 Whitefly Bemisia... 70 3e- 012 gb|EE600889.1|EE600889 BT-TYLCV-029-1-C7-T3_C07 Whitefly Bemisia... 70 3e- 012 gb|EE602477.1|EE602477 TYLCV-BT012_D09 Whitefly Bemisia tabaci (... 58 1e- 008 gb|EE674635.1|EE674635 BT-TYLCV-041-1-D6-T3_D06 Whitefly Bemisia... 58 1e- 008 gb|EE595697.1|EE595697 BT-H-016-1-G6-T3_G06 Whitefly Bemisia tab... 34 0.16 gb|EE598743.1|EE598743 BT-TOMOV-037-1-F2-T3_F02 Whitefly Bemisia... 32 0.65 gb|EE600967.1|EE600967 BT-TYLCV-031-1-A3-T3_A03 Whitefly Bemisia... 32 0.65 gb|EE602504.1|EE602504 TYLCV-BT012_G10 Whitefly Bemisia tabaci (... 32 0.65 gb|EE596971.1|EE596971 BT-H-046-1-B8-T3_B08 Whitefly Bemisia tab... 30 2.6 gb|EE597055.1|EE597055 HBT001_G10_T3_083 Whitefly Bemisia tabaci... 30 2.6 gb|EE597413.1|EE597413 HBT008_H04_T3_043 Whitefly Bemisia tabaci... 30 2.6 gb|EE597579.1|EE597579 HBT012_C07 Whitefly Bemisia tabaci (non-v... 30 2.6 gb|EE597651.1|EE597651 BT-TOMOV-014-1-A2-T3_A02 Whitefly Bemisia... 30 2.6 gb|EE597976.1|EE597976 BT-TOMOV-021-1-A9-T3_A09 Whitefly Bemisia... 30 2.6 gb|EE597993.1|EE597993 BT-TOMOV-021-1-D9-T3_D09 Whitefly Bemisia... 30 2.6 gb|EE598721.1|EE598721 BT-TOMOV-037-1-C3-T3_C03 Whitefly Bemisia... 30 2.6 gb|EE599181.1|EE599181 BT-TOMOV-046-1-D10-T3_D10 Whitefly Bemisi... 30 2.6 gb|EE599285.1|EE599285 TOMOV-BT004_B08 Whitefly Bemisia tabaci (... 30 2.6 gb|EE599555.1|EE599555 TOMOV-BT008_G05 Whitefly Bemisia tabaci (... 30 2.6 gb|EE599702.1|EE599702 TOMOV-BT011_D06 Whitefly Bemisia tabaci (... 30 2.6 gb|EE599852.1|EE599852 TMVBT001_H03_T3_031 Whitefly Bemisia taba... 30 2.6 gb|EE597702.1|EE597702 BT-TOMOV-015-1-B8-T3_B08 Whitefly Bemisia... 30 2.6 gb|EE598641.1|EE598641 BT-TOMOV-035-1-E3-T3_E03 Whitefly Bemisia... 30 2.6 gb|EE598640.1|EE598640 BT-TOMOV-035-1-D6-T3_D06 Whitefly Bemisia... 30 2.6 gb|EE598976.1|EE598976 BT-TOMOV-042-1-B5-T3_B05 Whitefly Bemisia... 30 2.6 gb|EE599031.1|EE599031 BT-TOMOV-043-1-C10-T3_C10 Whitefly Bemisi... 30 2.6 gb|EE599782.1|EE599782 TOMOV-BT012_F08 Whitefly Bemisia tabaci (... 30 2.6 gb|EE604533.1|EE604533 BT-HINST-048-1-A1-T3_H10 Whitefly Bemisia... 30 2.6 gb|EE599987.1|EE599987 BT_TYLCV002_E07 Whitefly Bemisia tabaci (... 30 2.6 gb|EE600250.1|EE600250 BT-TYLCV-014-1-H5-T3_H05 Whitefly Bemisia... 30 2.6 gb|EE601147.1|EE601147 BT-TYLCV-035-1-D9-T3_D09 Whitefly Bemisia... 30 2.6 gb|EE601232.1|EE601232 BT-TYLCV-037-1-B6-T3_B06 Whitefly Bemisia... 30 2.6 gb|EE601275.1|EE601275 BT-TYLCV-038-1-F5-T3_F05 Whitefly Bemisia... 30 2.6 gb|EE601849.1|EE601849 BT-TYLCV-055-1-E9-T3_E09 Whitefly Bemisia... 30 2.6 gb|EE601904.1|EE601904 BT-TYLCV-057-1-A12-T3_A12 Whitefly Bemisi... 30 2.6 gb|EE602110.1|EE602110 BT-TYLCV-062-1-F2-T3_F02 Whitefly Bemisia... 30 2.6 gb|EE602124.1|EE602124 BT-TYLCV-062-1-H12-T3_H12 Whitefly Bemisi... 30 2.6 gb|EE602283.1|EE602283 TYLCV009_B12 Whitefly Bemisia tabaci (rea... 30 2.6 gb|EE600523.1|EE600523 BT-TYLCV-020-1-H8-T3_H08 Whitefly Bemisia... 30 2.6 gb|EE600622.1|EE600622 BT-TYLCV-023-1-E2-T3_E02 Whitefly Bemisia... 30 2.6 gb|EE601269.1|EE601269 BT-TYLCV-038-1-C4-T3_C04 Whitefly Bemisia... 30 2.6 gb|EE602047.1|EE602047 BT-TYLCV-060-1-B7-T3_B07 Whitefly Bemisia... 30 2.6 gb|EE602180.1|EE602180 TYLCV006_B05 Whitefly Bemisia tabaci (rea... 30 2.6 gb|EE602418.1|EE602418 TYLCV-BT011_E08 Whitefly Bemisia tabaci (... 30 2.6

Query: 507 tgcaccaccaactgcctggctcccctggccaaggtcatcaatgacaacttcgagatcgtc 566 ||||| ||||||||| ||||||| || |||||||||||| | |||||||| | || || Sbjct: 130 tgcacaaccaactgcttggctcctctcgccaaggtcatccacgacaactt-taaattgtt 188

Query: 567 gagggtctgatgaccaccgtgcacgccaccactgccacccagaagac 613 ||||||||||||||||| || |||||||| || ||||| || ||||| Sbjct: 189 gagggtctgatgaccactgttcacgccacaaccgccacacaaaagac 235

Query: 399 gccaagaaggtcatcatctcggcccca 425 |||||||| ||||||||||| |||||| Sbjct: 24 gccaagaaagtcatcatctctgcccca 50

Query: 990 tggtacgacaacgagttcggttactccaaccgcgtcatcgacctgatcaagta 1042 |||||||| ||||||| |||||||||| | ||||| || ||||||||||| Sbjct: 605 tggtacgataacgagtatggttactccagcaaagtcatagatctgatcaagta 657 >gb|EE600889.1|EE600889 BT-TYLCV-029-1-C7-T3_C07 Whitefly Bemisia tabaci (reared on TYLCV infected plants) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 695

Query: 507 tgcaccaccaactgcctggctcccctggccaaggtcatcaatgacaacttcgagatcgtc 566 ||||| ||||||||| ||||||| || |||||||||| || |||||||| || || || Sbjct: 504 tgcacaaccaactgcttggctcctctcgccaaggtcaccac-gacaactttgaaattgtt 562

Query: 567 gagggtctgatgaccaccgtgcacgccaccactgccacc 605 ||||||||||||||||| || |||||||| || |||||| Sbjct: 563 gagggtctgatgaccactgttcacgccacaaccgccacc 601

Query: 363 accattgacaaggcgtccacccacttgaagggcggcgccaagaaggtcatcatctcggcc 422 ||||| |||||||| ||| | |||||| | || || |||||||| ||||||||||| ||| Sbjct: 362 accatcgacaaggcctccgctcacttggaaggtggtgccaagaaagtcatcatctctgcc 421

Query: 423 c 423 | Sbjct: 422 c 422

Query: 62 catgtcgaagatcggaattaacggatttggccg 94 |||||| || || ||||| |||||||||||||| Sbjct: 66 catgtctaacattggaatcaacggatttggccg 98 Gene 10

Query= gi|24656641|ref|NM_137698.1| Drosophila melanogaster CG9357 CG9357-RA (CG9357), mRNA (1448 letters)

Score E Sequences producing significant alignments: (bits) Value gb|EE597501.1|EE597501 HBT010_F10_T3_090 Whitefly Bemisia tabaci... 30 2.9 gb|EE597838.1|EE597838 BT-TOMOV-017-1-H7-T3_H07 Whitefly Bemisia... 30 2.9 gb|EE598826.1|EE598826 BT-TOMOV-039-1-C10-T3_C10 Whitefly Bemisi... 30 2.9 gb|EE602204.1|EE602204 TYLCV006_F12 Whitefly Bemisia tabaci (rea... 30 2.9 gb|EE602309.1|EE602309 TYLCV009_E11 Whitefly Bemisia tabaci (rea... 30 2.9 gb|EE600669.2|EE600669 BT-TYLCV-042-1-E2-T3_E02 Whitefly Bemisia... 30 2.9

>gb|EE597501.1|EE597501 HBT010_F10_T3_090 Whitefly Bemisia tabaci (non- viruliferous) cDNA library Bemisia tabaci cDNA 5', mRNA sequence Length = 251

Query: 1118 gatctggtcgctgga 1132 ||||||||||||||| Sbjct: 160 gatctggtcgctgga 174

Query: 1255 ccagtgaaggattca 1269 ||||||||||||||| Sbjct: 121 ccagtgaaggattca 107 Database: whitefly Posted date: Jun 20, 2008 3:06 PM Number of letters in database: 2,688,842 Number of sequences in database: 8903

Lambda K H 1.37 0.711 1.31

Gapped Lambda K H 1.37 0.711 1.31

Matrix: blastn matrix:1 -3 Gap Penalties: Existence: 5, Extension: 2 Number of Sequences: 8903 Number of Hits to DB: 27,664 Number of extensions: 9994 Number of successful extensions: 9994 Number of sequences better than 10.0: 140 Number of HSP's gapped: 9837 Number of HSP's successfully gapped: 196 Length of database: 2,688,842 X1: 11 (21.8 bits) X2: 15 (29.7 bits) X3: 25 (49.6 bits) S1: 11 (22.3 bits) S2: 14 (28.2 bits)