Table S1: Bacterial Strains Used in This Study

Table S1: Bacterial strains used in this study

Bacterial strain Resistance Description Source Streptococcus agalactiae NEM316 Wild-type (22) ΔrovS pBR322- This study pG+host8::updown.rov S → NEM316 Δshp pBR322- This study pG+host8::updown.shp → NEM316 ΔoppB pBR322- This study pG+host8::updown.opp B → NEM316 ΔpptAB pBR322- This study pG+host8::updown.ppt AB → NEM316 Δeep pBR322- This study pG+host8::updown.eep → NEM316 WT-pTCVlac Erm pTCV-lac NEM316 This study

WT-Pshp Erm Pshp NEM316 This study

ΔrovS-Pshp Erm Pshp ΔrovS This study

Δshp-Pshp Erm Pshp Δshp This study Δshp-pTCVlac Erm pTCV-lac Δshp This study

ΔoppB-Pshp Erm Pshp ΔoppB This study

WT-ProvS Erm ProvS NEM316 This study

ΔpptAB-Pshp Erm Pshp ΔpptAB This study

Δeep-Pshp Erm Pshp Δeep This study WT-P23shp Erm P23shp  NEM316 This study Δshp-P23shp Erm P23shp  Δshp This study Δeep-P23shp Erm P23shp  Δeep This study

WT-PfbsA Erm PfbsA NEM316 This study

ΔrovS-PfbsA Erm PfbsA ΔrovS This study

Δshp-PfbsA Erm PfbsA Δshp This study Escherichia coli TG1-dev Amp supE hsd-5 thi (lac-proAB)F’(traD36 (41) proAB+ lacIq lacZΔM15) repA+ - - - Rosetta Cm F ompT hsdSB(rB mB ) Novagen gal dcm pRARE TIL1387 Km pET28a::rovS → This study Rosetta