Curriculum Vitae

Personal Name: Karen V. Kibler, RBP, PhD Current Positions: Assistant Research Professor Assistant Director, Research Safety The Biodesign Institute Environmental Health and Safety Arizona State University Arizona State University Tempe, Arizona. Tempe, Arizona

Nationality: USA

Address: The Biodesign Institute Environmental Health and Safety 1001 S. McAllister, 310D 1551 S. Rural Road Arizona State University Arizona State University Tempe, AZ 85287-5401 Tempe, AZ 85281-5287 (480)727-0457 (480)727-0457 email: [email protected] email: [email protected]

Education 01/93-12/97, Ph.D. Arizona State University, Tempe, Arizona Department of Microbiology, Virology Degree received December, 1997 08/74-05/77, B.S. University of Iowa, Iowa City, Iowa Major: General Science (emphasis in chemistry) Degree received May, 1977 08/71-05/72 Drake University, Des Moines, Iowa Major: Secondary Education 08/70-05/71 Iowa Central Community College, Fort Dodge, Iowa Major: Music

Teaching Experience 08/02- 5/06 Faculty Associate School of Life Sciences, Arizona State University Techniques in Molecular Biology 01/93-12/97 Graduate Teaching Associate Department of Microbiology, Arizona State University General Microbiology Laboratory, 2 semesters Bacterial Genetics Laboratory, 1 semester Techniques in Molecular Biology, 3 semesters Microbiology (for non-majors), 1 semester

Research Experience 08/05 – current Assistant Research Professor, ASU, Tempe, AZ  Supervisor of BSL3 facility  Development of a new NYVAC construct as an HIV vaccine candidate

11/03 – 08/05 Faculty Research Associate, ASU, Tempe, AZ  Development of a Vaccinia virus-based HIV vaccine  Development of HIV replication inhibitors

01/02 – 10/03 Senior Research Fellow, Mayo Clinic, Scottsdale, AZ  Characterization of genetic variation in hepatitis C 5’ UTR.  Cell-specific infectivity of hepatitis C in HCV/HIV co-infected patients.

01/98-12/01 Research Fellow, National Institutes of Health, NIAID, LMM, Medical Virology Section  Study of HIV inhibition by synthetic peptides.  Study of MAD1 function in cancer.  Determination of cell cycle phase preference for expression from the HIV-I and HTLV-I LTRs.  Characterization of the repression of the Cyclin A promoter by HTLV-I Tax protein.

01/93-12/97 Graduate Research Associate, Department of Microbiology, Arizona State University  Characterization of the human Group C rotavirus NSP3 gene product.  Study of the vaccinia virus E3L gene product in the inhibition of induction of apoptosis.  Study of the rabbitpox and cowpox E3L gene product function in a serpin positive background.

Honors/Awards/Certifications: 2010 Registered Biosafety Professional certification from American Biological Safety Association 1998-2001 Recipient, NIH Office of Loan Repayment and Scholarship Program 1997 Graduate Academic Scholarship, Board of Regents, Arizona State University 1997 American Society for Microbiology, Arizona Branch, Student Presentation Award 1996-1997 Achievement Rewards for College Scientists (ARCS) 1996 Teaching Assistant of the Year Award, Microbiology Department, Arizona State University 1995-1996 Graduate Academic Scholarship, Board of Regents, Arizona State University 1995-1996 Associated Students of Arizona State University, GSC Outstanding College Council Member Award for Service 1995 American Society for Microbiology, Arizona Branch, Student Presentation Award 1994-1995 Graduate Academic Scholarship, Board of Regents, Arizona State University 1994-1995 Arizona State University Graduate Research Grant 1994-1995 College of Liberal Arts and Sciences, nomination for Distinguished Teaching Award for Teaching Assistants 1977 Honors Degree, University of Iowa

Assistant Director, Research Safety, Department of Environmental Health & Safety 03/11 – current  Management of the Select Agent facilities, including participation in the construction design; monitoring of engineering controls including, but not limited to ventilation and waste treatment systems for the biocontainment facilities; facilitation of re- commissioning.  Management of EH&S Compliance Officer Program, a network of ~120 safety representatives from ~85 institutes/schools/departments of ASU.  Participation in the ASU Pandemic Executive Group.  Voting member of the Institutional Animal Care and Use Committee.

Assistant Director, Biosafety & Biosecurity, Department of Environmental Health & Safety 09/06 – 03/11  Monitoring of compliance with all Federal, State, City and University biosafety regulations and guidelines through review of proposed research projects and inspection of laboratory facilities.  Development of biosafety training for all University personnel who perform biological work in labs at biosafety level 2 or higher, including Bloodborne Pathogens.  Maintenance of the Select Agent and Toxin Program at ASU.  Guidance on security measures for infectious agent laboratories.  Participation in the Influenza Pandemic Preparedness Plan for ASU.  Participation as voting member on the Institutional Biosafety Committee and the Institutional Animal Care and Use Committee.  Coordination of the preparation for the development and maintenance of manuals, policies and procedures, and related documents relative to biosafety and biosecurity, including medical surveillance for the researchers.  Performance of Health Hazard Assessments of infectious materials; feedback and instruction to protect the health and safety of the researchers, staff and students, as well as management of the university’s liability.  Participation in the construction design of biocontainment facilities. Monitoring of engineering controls including, but not limited to: ventilation and waste treatment systems for the biocontainment facilities.

Community/Student/Faculty Positions: 2006 -2007 School of Life Sciences Safety Committee 2001-2008 Scientific Advisory Board, Genome Justice Project, Nat’l Assoc. of Women Judges 1998-2000 Life Sciences judge for the Montgomery County (Maryland) Science Achievement Fair 1995-1996 Graduate Student Representative for students in the Department of Microbiology, representing students at faculty meetings, ASU 1995-1996 Graduate Student Council Representative for the Department of Microbiology, ASU 1995-1996 Chairperson of Peer Review Committee for awarding of Graduate Student Grants from the Graduate Research Support Office, ASU 1994-1995 Mentor in the Graduate College Academic Support Program, ASU

Selected Publications: Kibler, KV, Gomez, CE, Perdiguero, B, Wong, S, Huynh, T, Holechek, S, Arndt, W, Jimenez, V, Gonzalez- Sanz, R, Denzler, K, Haddad, EK, Wagner, R, Sekaly, RP, Tartaglia, J, Pantaleo, G, Jacobs, BJ, and Esteban, M. Improved NYVAC-Based Vaccine Vectors. PLoS ONE, 2011. E.D. Quakkelaar, A. Redeker, E.K. Haddad, A. Harari, S. Mayo McCaughey, T. Duhen, A. Filali-Mouhim, J.P. Goulet, N.M. Loof, F. Ossendorp, B. Perdiguero, P. Heinen, C.E. Gomez, K. Kibler, D.M. Koelle5, R.P. Sékaly, F. Sallusto, A. Lanzavecchia, G. Pantaleo, M. Esteban, J. Tartaglia, B. Jacobs and C.J.M. Melief. Improved innate and adaptive immunostimulation by genetically modified HIV-1 protein expressing NYVAC vectors. PLoS ONE, 2010. Tsen, K.T., Tsen, S.W., Fu, Q., Lindsay, S.M., Kibler, K., Jacobs, B., Wu, T.C., Karanam, B., Jagu, S., Roden, R.B., Hung, C.F., Sankey, O.F., Ramakrishna, B. and Kiang, J.G. (2009) Photonic approach to the selective inactivation of viruses with a near-infrared subpicosecond fiber laser. J Biomed Opt 14(6), 064042. Jentarra GM, Heck MC, Youn JW, Kibler K, Langland JO, Baskin CR, Ananieva O, Chang Y, Jacobs BL. 2008. Vaccinia viruses with mutations in the E3L gene as potential replication-competent, attenuated vaccines: scarification vaccination. Vaccine, 26(23):2860-72. Gallegos-Orozco JF, Arenas JI, Vargas HE, Kibler KV, Wilkinson JK, Nowicki, M, Radkowski M, Nasseri J, Rakela J, Laskus T. 2006. Selection of different 5'untranslated region hepatitis C virus variants during post-transfusion and post-transplantation infection. J Viral Hep, 13(7):489-98. Haller, K., K.V. Kibler, T. Kasai, Y. Chi, J.-M. Peloponese, V. Yedavalli, and K.-T. Jeang. 2006. The N- terminus of rodent and human MAD1 confers species-specific stringency to spindle assembly checkpoint. Oncogene, 25(15):2137-47. K.V. Kibler, A. Miyazato, V.R. Yedavalli, A.I. Dayton, B.L. Jacobs, G. Dapolito, S. Kim, and K.-T. Jeang. 2004. Polyarginine inhibits gp160 processing by furin and suppresses productive human immunodeficiency virus type 1 infection. Journal of Biological Chemistry, 279(47):49055-63. J.I. Arenas, J.F.Gallegos-Orozco, T. Laskus, J. Wilkinson, A. Khatib, C. Fasola, D. Adair, M. Radkowski, K.V. Kibler, M. Nowicki, D. Douglas, J. Williams, G. Netto, D. Mulligan, G. Klintmalm, J. Rakela, H.E. Vargas. 2004. Hepatitis C virus quasi-species dynamics predict progression of fibrosis after liver transplantation. Journal of Infectious Diseases, 189(11):2037-46. T. Laskus, M. Radkowski, J. Jablonska, K. Kibler, J. Wilkinson, D. Adair, J. Rakela. 2004. Human immunodeficiency virus facilitates infection/replication of hepatitis C virus in native human macrophages. Blood, 103(10):3854. H. Iha, K.V. Kibler, V.R. Yedavalli, J.M. Peloponese, K. Haller, A. Miyazato, T. Kasai, K.T. Jeang. 2003. Segregation of NF-kappaB activation through NEMO/IKKgamma by Tax and TNFalpha: implications for stimulus-specific interruption of oncogenic signaling. Oncogene, 22(55):8912-23. S. Hwang, N. Tamilarasu, K. Kibler, H. Cao, A. Ali, Y.H. Ping, K.T. Jeang, T.M. Rana. 2003. Discovery of a small molecule Tat-trans-activation-responsive RNA antagonist that potently inhibits human immunodeficiency virus-1 replication. Journal of Biological Chemistry, 278(40):39092-103. M. Lohr, K.V. Kibler, I. Zachary, K.T. Jeang, and D.L. Selwood. 2003. Small HIV-1-Tat peptides inhibit HIV replication in cultured T-cells. Biochem Biophys Res Commun, 300(2):609-613. Y. Iwanaga, T. Kasai, K. Kibler, and K.T. Jeang. 2002. Characterization of Regions in hsMAD1 Needed for Binding hsMAD2. A Polymorphic Change in an hsMAD1 Leucine Zipper Affects MAD1-MAD2 Interaction and Spindle Checkpoint Function. Journal of Biological Chemistry, 277(34):31005-13. H. Iha, T. Kasai, K.V. Kibler, Y. Iwanaga, and K.T. Jeang. 2000. Pleiotropic Effects of HTLV-I Tax Protein on Cellular Metabolism: Examples of Mitotic Checkpoint Abrogation and NF-B Activation. Aids Research and Human Retroviruses, 16:1633-38. D.Y. Jin, H.-L. Wang, Y. Zhou, A.C.S. Chun, K.V. Kibler, Y.-D. Hou, H. Kung, and K.T. Jeang. 2000. Hepatitis C virus core protein-induced loss of LZIP function correlates with cellular transformation. The EMBO Journal, 19:729-740. K.V. Kibler and K. T. Jeang. 1999. Taxing the Cellular Capacity for Repair: Human T-Cell Leukemia Virus Type 1, DNA Damage, and Adult T-Cell Leukemia. Journal of the National Cancer Institute, Editorials, 91(11):903-904. D.Y. Jin, V. Giordano, K.V. Kibler, H. Nakano, and K.T. Jeang. 1999. Role of Adapter Function in Oncoprotein-mediated Activation of NF-B. The Journal of Biological Chemistry, 274(25):17402- 17405. K. V. Kibler, T. Shors, K. B. Perkins, C.C. Zeman, M.P. Banaszak, J. Biesterfeldt, J. O. Langland, and B. L. Jacobs. 1997. Double-Stranded RNA Is a Trigger for Apoptosis in Vaccinia Virus-Infected Cells. Journal of Virology, 71(3):1992-2003.