Table S1. Proteases inhibited by TLCK, TPCK, PMSF, MG-132, PSI, LC, CLC, Ca I I and Ca I II. A) Reported IC 50

for the proteasome and calpains. B) Reported IC50 for the other cysteine and serine proteases

Table 1 A

Inhibitor Effect on proteases, IC50 (M) Proteasome Calpains Cht-Ac T-Ac Pg-Ac (C-Ac) Ca I Ca II

TLCK NE [1] NE [1] TPCK NE [1] NE [1] PMSF NE [2] NE [2] NE [2] MG-132 * < 10/ 0.85 [3]/[4] > 20 [4] NE [4] 1.2 [4] PSI 0.25-6.5 [5] > 65 [5] > 65 [5] 4 [5]

LC < 1 < 1 [6; 7] < 1 [6; 7] NE [8] NE [8] [6; 7] CLC < 1 [6; 7] < 1 [6; 7] < 1 [6; 7] CaI I * 0.14 / 10 [9] / [10] 0.67 / > 50 [9] / [10] 1.2/ > 50 [9] / [10]] 0.005/0.19 [9]/ [11] 0.22 [11] CaI II * 1/ 25 [9; 10] 28 / > 50 [9; 10] > 50 [10] 0.009/ 0.12 [9]/ [11] 0,23 [11]

1 Table 1 B

Inhibitor Effect on proteases, IC50 (M) Cysteine proteases Serine proteases Cat A-Ac Cat L Cat B Cat D Cat H Pap Trypsin Cht Pl PA

TLCK + [12] + [13; 14] NE [13] TPCK + [12] NE [13] + [13] PMSF + [15] + [16] + [16] MG-132 PSI LC < 1 [17] NE [8] NE [8] NE [8; 13] NE [13] NE [8] CLC NE [13] NE [13] CaI I 0.007/0.0005 0.005-0.012/0.013/0.15 9.4 > 10 [11] > 250 /NE [18]/ [11] > 250/ ¤ [18]/ [11] > 250 [18] [18]/ [Sasaki, [18]/[9]/[11] [18] 1990] CaI II 0.03/ 0.0006 0.005-0.012/0.06 / 0.1 360 > 10 [11] > 250/ NE [18]/ [11] > 250/ ¤¤ [18]/ [11] > 250 [18] [18]/ [11] [18]/ [9]/ [11] [18]

Abbreviations: Cht-Ac= chymotrypsin-like activity, T-Ac: trypsin-like activity; Pg-Ac (C-Ac): peptidyl-glutamyl-like activity (caspase-like activity); Ca I: calpain I; Ca II: calpain II; Cat A-Ac: cathepsin A-like activity; Cat L: cathepsin L; Cat B: cathepsin B; Cat D: cathepsin D; Cat H: cathepsin H; Pap: papain; Cht: chymotrypsin; Pl: plasmin; PA: plasminogen

activator; NE: no effect ; + : inhibitor but IC50 not reported; ¤ : ~ 50 % inhibition at 50 M [11]; * : MG-132, Ca I I and Ca I II

are also potent inhibitors of the caseinolytic activity of the proteasome (IC50 = 0.1, 7 and 12 M, respectively [4; 5]; ¤¤ : ~ 30 % inhibition at 50 M [11]. References are bold numbered in brackets..

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