Title: Genes, Color, and Geography: the Evolutionary Enigma of the Pygmy Angelfishes From

Electronic Supplementary Material

Appendix S1 Material examined GenBank Acc. # Museum Voucher Tissue ID SL Sample location Sample date Collector COI, Cyt b

AMS I. 17313-004 n/a 134.5 Off Brunswick, NSW (S 28˚ 32’, E 152˚ August 1973 T. Wongratana, NSW State n/a 37’) Fisheries

WAM P28332-001 n/a 146.0 100 M of N Mole, Fremantle, Western July 1984 N. Sinclair n/a Australia (S 32˚ 03’, E 115˚ 44’)

WAM P28332-001 n/a 100 M of N Mole, Fremantle, Western July 1984 N. Sinclair n/a 146.0 Australia (S 32˚ 03’, E 115˚ 44’)

CSIRO C2647 n/a 180.0 Oyster Harbour, Albany, Western November 1954 n/a n/a Australia (S 34˚ 58’, E 117˚ 57’)

CSIRO CA182 n/a 133.5 Jervis Bay, NSW (S 85˚ 00’, E 150˚ 45’) July 1977 I.S.R. Munro n/a

CSIRO CA183 n/a 131.0 Jervis Bay, NSW (S 85˚ 00’, E 150˚ 45’) July 1977 I.S.R. Munro n/a

WAM P32528.001 n/a 182.5 Rottnest Island, Perth Canyon, Western February 2004 G.I. Moore, “RV Southern n/a Australia (S 31˚ 57’, E 115˚ 08’) Surveyor” 2

WAM P32528.001 n/a 183.0 Rottnest Island, Perth Canyon, Western February 2004 G.I. Moore, “RV Southern n/a Australia (S 31˚ 57’, E 115˚ 08’) Surveyor”

CSIRO C1934 n/a 133.0 Lake Illawarra, NSW (S 34˚ 32’, E 150˚ January 1940 I.S.R. Munro n/a 50’) 3 miles off Clarence River, NSW (S 29˚ KM116360, AMS I.45555-001 Eter113 119.5 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116286 3 miles off Clarence River, NSW (S 29˚ KM116361, AMS I.45555-001 Eter114 115.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116287 3 miles off Clarence River, NSW (S 29˚ KM116362, AMS I.45555-001 Eter115 124.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116288 3 miles off Clarence River, NSW (S 29˚ KM116363, AMS I.45555-001 Eter116 116.5 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116289 3 miles off Clarence River, NSW (S 29˚ KM116364, AMS I.45555-001 Eter117 120.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116290 3 miles off Clarence River, NSW (S 29˚ KM116365, AMS I.45555-001 Eter118 130.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116291 3 miles off Clarence River, NSW (S 29˚ KM116366, AMS I.45555-001 Eter119 117.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116292 3 miles off Clarence River, NSW (S 29˚ KM116367, AMS I.45555-001 Eter120 119.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116293 3 miles off Clarence River, NSW (S 29˚ KM116368, AMS I.45555-001 Eter121 123.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116294 3 miles off Clarence River, NSW (S 29˚ KM116369, AMS I.45555-001 Eter122 119.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116295 3 miles off Clarence River, NSW (S 29˚ KM116370, AMS I.45555-001 Eter123 127.5 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116296 3 miles off Clarence River, NSW (S 29˚ KM116371, AMS I.45555-001 Eter124 117.0 July 2011 “Commercial Prawn Trawler” 25’ 50”, E 153˚ 25’ 25”) KM116297 3 miles off Clarence River, NSW (S 29˚ AMS I.45555-001 n/a 129.5 July 2011 “Commercial Prawn Trawler” n/a 25’ 50”, E 153˚ 25’ 25”) 3

3 miles off Clarence River, NSW (S 29˚ AMS I.45555-001 n/a 124.0 July 2011 “Commercial Prawn Trawler” n/a 25’ 50”, E 153˚ 25’ 25”) 3 miles off Clarence River, NSW (S 29˚ AMS I.45555-001 n/a 120.0 July 2011 “Commercial Prawn Trawler” n/a 25’ 50”, E 153˚ 25’ 25”)

SAM F12433 Eter 99 151.0 Buffalo Reef, Spencer Gulf, South 2011 A. Ivey, SARDI KM116385, Australia (S 34˚ 43’ 1”, E 136˚ 27’ 31”) KM116311

SAM F12434 Eter 100 140.0 Buffalo Reef, Spencer Gulf, South 2011 A. Ivey, SARDI KM116372, Australia (S 34˚ 43’ 1”, E 136˚ 27’ 31”) KM116298

SAM F12435 Eter 101 148.5 Thistle Island, Spencer Gulf, South 2011 A. Ivey, SARDI KM116373, Australia (S 35˚ 1’, E 136˚ 9’) KM116299

SAM F12436 Eter 102 122.0 Wardang Island, Spencer Gulf, South 2011 A. Ivey, SARDI KM116374, Australia (S 34˚ 30’, E 137˚ 22’) KM116300

SAM F12439 Eter 105 154.5 Thistle Island, Spencer Gulf, South 2011 A. Ivey, SARDI KM116377, Australia (S 35˚ 1’, E 136˚ 9’) KM116303 Thistle Island, Spencer Gulf, South KM116378, SAM F12440 Eter 106 149.5 2011 A. Ivey, SARDI Australia (S 35˚ 1’, E 136˚ 9’) KM116304 Thistle Island, Spencer Gulf, South KM116379, SAM F12441 Eter 107 148.0 2011 A. Ivey, SARDI Australia (S 35˚ 1’, E 136˚ 9’) KM116305 Thistle Island, Spencer Gulf, South KM116380, SAM F12442 Eter 108 148.5 2011 A. Ivey, SARDI Australia (S 35˚ 1’, E 136˚ 9’) KM116306 Thistle Island, Spencer Gulf, South KM116381, SAM F12443 Eter 109 142.0 2011 A. Ivey, SARDI Australia (S 35˚ 1’, E 136˚ 9’) KM116307 Thistle Island, Spencer Gulf, South KM116382, SAM F12444 Eter 110 146.0 2011 A. Ivey, SARDI Australia (S 35˚ 1’, E 136˚ 9’) KM116308 Thistle Island, Spencer Gulf, South KM116383, SAM F12445 Eter 111 148.0 2011 A. Ivey, SARDI Australia (S 35˚ 1’, E 136˚ 9’) KM116309 4

Thistle Island, Spencer Gulf, South KM116384, SAM F12446 Eter 112 146.0 2011 A. Ivey, SARDI Australia (S 35˚ 1’, E 136˚ 9’) KM116310 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116386, BPBM 41163 Eter125 216.0 35˚ 02’, E 117˚ 56’) Western Australia KM116312 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116387, BPBM 41163 Eter126 195.5 35˚ 02’, E 117˚ 56’) Western Australia KM116313 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter127 209 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116388, BPBM 41163 Eter128 194.5 35˚ 02’, E 117˚ 56’) Western Australia KM116314 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116389, BPBM 41163 Eter129 190.0 35˚ 02’, E 117˚ 56’) Western Australia KM116315 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116390, BPBM 41163 Eter130 206.5 35˚ 02’, E 117˚ 56’) Western Australia KM116316 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116391, BPBM 41163 Eter131 195.5 35˚ 02’, E 117˚ 56’) Western Australia KM116317 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116392, BPBM 41163 Eter132 190.0 35˚ 02’, E 117˚ 56’) Western Australia KM116318 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116393, BPBM 41163 Eter133 189.0 35˚ 02’, E 117˚ 56’) Western Australia KM116319 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter134 198.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116394, BPBM 41163 Eter135 194.0 35˚ 02’, E 117˚ 56’) Western Australia KM116320 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter136 196.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116395, BPBM 41163 Eter137 206.0 35˚ 02’, E 117˚ 56’) Western Australia KM116321 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116396, BPBM 41163 Eter138 204.5 35˚ 02’, E 117˚ 56’) Western Australia KM116322 5

King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116397, BPBM 41163 Eter139 179.5 35˚ 02’, E 117˚ 56’) Western Australia KM116323 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116398, BPBM 41163 Eter140 210.0 35˚ 02’, E 117˚ 56’) Western Australia KM116324 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116399, BPBM 41163 Eter141 211.5 35˚ 02’, E 117˚ 56’) Western Australia KM116325 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116400, BPBM 41163 Eter142 203.0 35˚ 02’, E 117˚ 56’) Western Australia KM116326 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter143 187.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116401, BPBM 41163 Eter144 218.0 35˚ 02’, E 117˚ 56’) Western Australia KM116327 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116402, BPBM 41163 Eter145 208.5 35˚ 02’, E 117˚ 56’) Western Australia KM116328 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116403, BPBM 41163 Eter146 179.0 35˚ 02’, E 117˚ 56’) Western Australia KM116329 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116404, BPBM 41163 Eter147 192.0 35˚ 02’, E 117˚ 56’) Western Australia KM116330 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116405, BPBM 41163 Eter148 205.0 35˚ 02’, E 117˚ 56’) Western Australia KM116331 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116406, BPBM 41163 Eter149 187.5 35˚ 02’, E 117˚ 56’) Western Australia KM116332 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116407, BPBM 41163 Eter150 216.0 35˚ 02’, E 117˚ 56’) Western Australia KM116333 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116408, BPBM 41163 Eter151 202.0 35˚ 02’, E 117˚ 56’) Western Australia KM116334 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116409, BPBM 41163 Eter152 186.0 35˚ 02’, E 117˚ 56’) Western Australia KM116335 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116410, BPBM 41163 Eter153 205.0 35˚ 02’, E 117˚ 56’) Western Australia KM116336 6

King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116411, BPBM 41163 Eter154 188.0 35˚ 02’, E 117˚ 56’) Western Australia KM116337 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116412, BPBM 41163 Eter155 205.0 35˚ 02’, E 117˚ 56’) Western Australia KM116338 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116413, BPBM 41163 Eter156 221.0 35˚ 02’, E 117˚ 56’) Western Australia KM116339 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter157 208.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter158 197.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116414, BPBM 41163 Eter159 208.0 35˚ 02’, E 117˚ 56’) Western Australia KM116340 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116415, BPBM 41163 Eter160 183.0 35˚ 02’, E 117˚ 56’) Western Australia KM116341 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116416, BPBM 41163 Eter161 215.0 35˚ 02’, E 117˚ 56’) Western Australia KM116342 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116417, BPBM 41163 Eter162 198.0 35˚ 02’, E 117˚ 56’) Western Australia KM116343 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116418, BPBM 41163 Eter163 180.0 35˚ 02’, E 117˚ 56’) Western Australia KM116344 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116419, BPBM 41163 Eter164 170.0 35˚ 02’, E 117˚ 56’) Western Australia KM116345 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116420, BPBM 41163 Eter165 158.0 35˚ 02’, E 117˚ 56’) Western Australia KM116346 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116421, BPBM 41163 Eter166 153.0 35˚ 02’, E 117˚ 56’) Western Australia KM116347 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116422, BPBM 41163 Eter167 141.0 35˚ 02’, E 117˚ 56’) Western Australia KM116348 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116423, BPBM 41163 Eter168 158.0 35˚ 02’, E 117˚ 56’) Western Australia KM116349 7

King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116424, BPBM 41163 Eter169 142.0 35˚ 02’, E 117˚ 56’) Western Australia KM116350 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116425, BPBM 41163 Eter170 163.0 35˚ 02’, E 117˚ 56’) Western Australia KM116351 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116426, BPBM 41163 Eter171 156.0 35˚ 02’, E 117˚ 56’) Western Australia KM116352 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116427, BPBM 41163 Eter172 165.5 35˚ 02’, E 117˚ 56’) Western Australia KM116353 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116428, BPBM 41163 Eter173 155.0 35˚ 02’, E 117˚ 56’) Western Australia KM116354 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116429, BPBM 41163 Eter174 179.0 35˚ 02’, E 117˚ 56’) Western Australia KM116355 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116430, BPBM 41163 Eter175 166.0 35˚ 02’, E 117˚ 56’) Western Australia KM116356 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter176 170.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116431, BPBM 41163 Eter177 159.0 35˚ 02’, E 117˚ 56’) Western Australia KM116357 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter178 172.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116432, BPBM 41163 Eter179 162.0 35˚ 02’, E 117˚ 56’) Western Australia KM116358 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter180 173.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries KM116433, BPBM 41163 Eter181 167.0 35˚ 02’, E 117˚ 56’) Western Australia KM116359 King George Sound, Western Australia (S August 25 2011 T. Leary, Department of Fisheries BPBM 41163 Eter182 170.0 n/a 35˚ 02’, E 117˚ 56’) Western Australia