Laboratory 2: Tools For Scientific Inquiry

Laboratory 7: Cellular Respiration

Name: ______Group: ______Date:______

7.1 Alcoholic Fermentation Name four factors that can affect enzyme activity: ______, ______, ______, and ______.

Data Table 7.2: Results of Fermentation Experiment (mm CO2 produced) 0 5 10 15 20 25 30 Tube min min min min min min min 1 (3 ml corn syrup) 2 (3 ml corn syrup + 1 ml yeast) 3 (3ml corn syrup + 3 ml yeast) Describe your experiment below: Dependent Variable: ______Independent Variable: ______What hypothesis is being tested?

Predict the outcome of the experiment.

Was your hypothesis proven false or supported by the results? Use data to support your answer.

Which fermentation tube was the control? ______Why were different amounts of water added to each fermentation tube?______What are some other independent variables that could affect alcoholic fermentation?

Figure 7.4: Rate of carbon dioxide production by yeast fermentation. Plot your data from table 7.2 on the following graph. Add values on X and Y axis. 2 O C m m

Time (min) 7.2 Aerobic Respiration What is the role of the following components of the reaction mixture? Lima bean extract: ______Succinate: ______DCPIP: ______Buffer: ______What is the independent variable? ______What is the dependent variable? ______What hypothesis is being tested?

Predict the outcome of the experiment in terms of your hypothesis.

Data Table 7.4: Results of Mitochondrial Activity Experiment (% Transmittance) 0 5 10 15 20 25 30 Tube min min min min min min min 1 (No succinate) 2 (0.2 ml succinate) 3 (0.4 ml succinate)

Figure 7.10: Rate of aerobic respiration in mitochondria. Add values on X and Y axis. e c n a t t i m s n a r T

Time (min)

Do your results support your hypothesis or prove it to be false? ______

Which reaction tube was the control? ______Why should the succinate be added last?

What are some other independent variables that could affect aerobic respiration?