ROTUNDA HOSPITAL DEPARTMENT OF LABORATORY MEDICINE Biochemistry Active Test Repertoire Table LF-GEN-0066Ed 00 This active test table informs the user of Biochemistry tests available in house. Tests referred to other sites are recorded in the Referred Test Table. Issue date: 4TH April 2016 Contact Phone Number ext.1345/2522 No. Test Sample Special Precautions Reference Range INAB Routine Type Accredited Turnaround Test Time Reg No. 208MT 1. Alanine Adult Female : <33U/L  Aminotran Adult Male : < 41 U/L sferase Neonates: <45 U/L (ALT) 2. Albumin Non pregnant/Male: 35 - 50 g/L  Pregnant: 23 - 42 g/L Neonate: 32 - 48 g/L 3. Alkaline Adult Female : 35 -105 U/L  Phosphata Adult Male: 45-130 U/L se Pregnant: 20-230 U/L (ALP) Neonate: Lithium Sample should arrive in 2-3 hrs <10 days: 45-245 U/L Heparin laboratory within 4 hrs < 4 weeks 45-315 U/L < 1Y 60-550 U/L < 9Y 50-450 U/L <15Y 50-350 U/L 4. Amylase Adult: 28-100 U/L  Neonate: <100 U/L 5. Aspartate Adults female: <32 U/L  Aminotran Adult Male: <45 U/L sferase Neonate: (AST) < 4 weeks: < 75U/L < 1Y: <65U/L < 4Y <50 U/L 6. Bile Acids Serum or Minimum 4hr fast. Adult Female 0 - 10 umo/L  Lithium Please state fasting

Page 1 of 9 Ref: lpgen0007 No. Test Sample Special Precautions Reference Range INAB Routine Type Accredited Turnaround Test Time Reg No. 208MT Heparin status on request form Bilirubin  7. <5 µmol/L Direct 8. Bilirubin Sample should arrive in Adult: <21 mol/L  Total laboratory within 4 hrs Neonates: <17mol/L 9. Calcium Adults: 2.15 - 2.50 mmol/L  Neonates: 2.15 - 2.65 mmol/L 10. Chloride Adult: 98 -107 mmol/L  Neonates: 98 -110 mmol/L Urine : No range quoted Cholester Overnight fast Adults: <5.2 mmol/L  11. Lithium ol 2-3 hrs Heparin 12. C- <10 mg/ L  Reactive Protein 13. Creatine Adult Female: 20 - 180U/L  Kinase Adult Male 20 - 200 (CK) Neonate: < 2weeks : <600U/L Sample should arrive in <1 month: <450 U/L laboratory within 4 hrs <1yr : <300 U/L 14. Creatinine Adult Female: 45 - 85 µmol/L  Adult Male: 60 - 105 µmol/L Pregnant: 45 - 80 µmol/L < 7 days: <100 µmol/L < 4 weeks: 10 - 70 µmol/L < 1yr : 10 - 70 µmol/L < 4 years: 15 - 45 µmol/L 15. Fructosam Serum or 205 - 285 µmol/l  ine Lithium Heparin 2-3 hrs ROTUNDA HOSPITAL DEPARTMENT OF LABORATORY MEDICINE Biochemistry Active Test Repertoire Table LF-GEN-0066Ed 00 No. Test Sample Special Precautions Reference Range INAB Routine Type Accredited Turnaround Test Time Reg No. 208MT 16. Gentamici Dependant on dose and time of  n dose. 17. GGT Sample should arrive in Adult Female : 6 - 42 U/L  Lithium laboratory within 4 hrs Adult Male: 10 - 71 U/L Heparin Neonate: <1 month: 0 - 150 U/L 1 - 2 month : 0 - 114 U/L 2 - 4 month : 0 - 81 U/L 4 - 7 month : 0-34 U/L <12yr : 0 - 24 U/L 18. Glucose Adult fasting: < 5.1 mmol/L  19. Glucose Collection time must be Pregnant  Tolerance Sodium handwritten on tube and Fasting: < 5.1 mmol/L Test Fluoride on request form. 1 Hour: < 10 mmol/L 2 Hour: < 8.5 mmol/L 20. Breakfast Fasting: < 5 mmol/L  Club 1 hour: < 7 mmol/L Haemoglo EDTA Ethnicity must be Non Pregnant: 20 - 42 mmol/mol  21. 1 week bin A1c included on request form 22. HDL - Overnight fast 2-3 hours 1.0-2.2 mmol/L  Cholester ol 23. LDL - 2.0 - 3.36  Cholester Lithium ol

Page 3 of 9 Ref: lpgen0007 No. Test Sample Special Precautions Reference Range INAB Routine Type Accredited Turnaround Test Time Reg No. 208MT 24. Lactate Heparin Adult : 245 - 480 U/L  Dehydrog Sample should arrive in Neonate: enase laboratory within 4 hrs <30 d: 365 - 1450 U/L (LDH) 30d-6mth: 310 - 790 U/L 6mth-1yr : 325 - 670 U/L >1yr - 2yr: 200 - 565 U/L >2yr - 16yr : 230 - 600 U/L

25. Lactate Sodium Send to the lab 2-3 hours 0.5 - 2.2 mmol/L  Fluoride immediately 26. Magnesiu Adult: 0.7-1.1 mmol/L  m Lithium Neonates: 0.6-1.0 mmol/L 27. Phosphate Heparin Adult: 0.8-1.45 mmol/L  Neonates: 1.5-2.55 mmol/L 28. Potassium Adult: 3.4-4.5mmol/L  Neonates: 3.5-5.5 mmol/L Urine : No range quoted 29. Sodium Lithium Non pregnant: 136 -145 mmol/L  Heparin / Pregnant: 133 -143 mmol/L Urine Neonate: 133 -144 mmol/L Urine : No range quoted 30. Total Lithium Sample should arrive in Non pregnant: 65 - 90 g/L  Protein Heparin the laboratory within 4 Pregnant: 56 - 76 g/l hrs Neonate: 60 -80 g/L 31. Total 24 hr Urine Adult : < 0.15g/L  Protein collection Urine (24hr collection) 32. Creatinine 24 hr urine & Non Pregnant: 80 -125 ml/min  Clearance Lithium Hep ROTUNDA HOSPITAL DEPARTMENT OF LABORATORY MEDICINE Biochemistry Active Test Repertoire Table LF-GEN-0066Ed 00 No. Test Sample Special Precautions Reference Range INAB Routine Type Accredited Turnaround Test Time Reg No. 208MT (24hr collection) 33. Protein / Spot Urine < 15 mg/mmol Cr Creatinine Normal to mildly increased Ratio 15 - 50 mg/mmol Cr Moderately Increased > 50 mg/mmol Cr Severely Increased NICE guidelines do not apply to pregnant women. PET women with PCR > 30 mg/mmol Cr should be confirmed with 24 hr urine collection 34. CSF CSF 2-3 hours CSF 0.1-0.4 g/L  Protein 35. CSF 2.5-4.0 mmol/L  Glucose Send to the lab 36. CSF CSF in immediately Adult : 1.1 - 2.4 Lactate sodium Neonate : fluoride tube 0-3d : 1.1 - 6.7 mmol/L 3d-10d : 1.1 - 4.4 mmol/L 10d - 2yrs: 1.1 - 2.8 mmol/L >2yrs : 1.1 - 2.4 mmol/L 37. Triglycerid Lithium Overnight fast Adults: <2.26 mmol/L  es Heparin Neonate: < 1.7mmol/L 38. Urea Non-pregnant 2.8-8.1mmol/L  Pregnant: 1.0-3.8 mmol/L Sample should arrive in Neonates: 1.0 - 6.0 mmol/L 39. Uric Acid Lithium laboratory within 4 hrs Non pregnant :145 - 345 mol/L  Heparin Pregnant: 120 - 375 mol/L

Page 5 of 9 Ref: lpgen0007 No. Test Sample Special Precautions Reference Range INAB Routine Type Accredited Turnaround Test Time Reg No. 208MT Male: 200 - 420 mol/L Neonates 150 - 330 mol/L ROTUNDA HOSPITAL DEPARTMENT OF LABORATORY MEDICINE Biochemistry Active Test Repertoire Table LF-GEN-0066Ed 00 Endocrinology No. Test Sample Type Routine Ur Reference Range INAB Turnaround Time ge Accredited nt Test Ti me s 1. Anti Thyroid-Specific Peroxidase Serum Same day N/ 0-34kU/L  (except at A Only performed on weekends) 1. Pregnant patients with TSH>2.0 mIU/L 2. Non pregnant patients with TSH > 4.0 mIU/L 2. AMH Serum Run twice weekly N/ Adult Female: Neonate Female:  A 13.1 - 53.8 20 -29 yrs 3.6 - 24 <3m 6.8 - 47.8 30 -34 yrs 2.4 - 15 <12m 5.5 - 37.4 35 -39 yrs 1.5 - 46 <8Y 0.7 - 21.2 45 -44 yrs 0.3 - 14.7 45 -50 yrs Neonate Male: 111 - 347 <15d 599 - 1544 <6m 279 - 651 <1Y 367 - 631 <4Y 316 - 1118 <6Y 257 - 974 <9Y

Serum

Serum 3. CA-125 Run twice weekly 0 -3 5 kU/L 

4. Ferritin Same day Female: 13 - 150 g/L  Male: 30 - 450 g/L

Page 7 of 9 Ref: lpgen0007 No. Test Sample Type Routine Ur Reference Range INAB Turnaround Time ge Accredited nt Test Ti me s 5. Folate (except at N/ 4.6-18.7 ng/ml  weekends) A 6. Follicle Stimulating Hormone Serum See table below  7. Free Thyroxine (FT4) Adult: 12-22pmol/L  2 < 1 month: 10-36pmol/L ho < 1 year: 10-26pmol/L 8. Human ChoronicGonadotrophin ursMale and non-pregnant females: < 1.0 U/L  Pregnant female: See below 9. Lutenizing Hormone See table below  10. Oestradiol  Same day 11. Progesterone  (except at N/ 12. Prolactin weekends) A Female: 100 - 500 mIU/L  Male: 85 - 325 mIU/L 13. Sex Hormone Binding Globulin Female: 32 - 128 nmol/LL  Male: 18 - 54 nmol/L 14. Testosterone Female: 0.2 - 3.0 nmol/L  Male: 9 - 29 nmol/L 15. Thyroid Stimulating Hormone 2 Adult: 0.1 - 4.0 mIU/L  ho < 2 days: 5.0 - 45 mIU/L urs < 11 years: 0.1-5.5 mIU/L 16. Vitamin B12 N/ Adult: 141 - 489 pg/ml  A ROTUNDA HOSPITAL DEPARTMENT OF LABORATORY MEDICINE Biochemistry Active Test Repertoire Table LF-GEN-0066Ed 00

Reference Ranges for Fertility Hormones Analyte Unit Male Follicular Phase Midcycle Luteal phase Post-menopausal LH U/L 1-9 2-13 14-96 1-12 8-60 FSH U/L 1-12 3-12 5-25 1-8 25-135 Oestradiol pmol/L - 45-854 - - <183 Progesterone nmol/L 0.7-4.3 0.6-4.7 2.4-9.4 > 20 0.3-2.5

Average HCG (U/L) and days of cyesis (days post LMP) – Royal Berkshire Hospital figures:

Day HCG Day HCG Day HCG Day HCG 24 50 25 63 26 80 27 102 28 135 29 180 30 245 31 320 32 420 33 545 34 710 35 945 36 1300 37 1700 38 2300 39 3100 45 4300 41 6200 42 8200 43 11000 44 14500 45 18000 45 23000 47 28000 48 34500 49 45000 50 46000 51 51000 52 56000 53 62000 54 68000 55 74500 56 80000 57 86000 58 91000 59 96000 60 101000

Figures are based on data collected between December 1996 and February 1997 in the Endocrinology Department, RBH, from apparently normal pregnancies. There is considerable individual variation, but around 50% of normal pregnancies give HCG values within 3 days of the average day, and around 80% within 1 week of the average day. After 60 days of cyesis, HCG values plateau and being to decline. HCG results cannot therefore be used to predict dates for pregnancies of duration likely to be more than 60 days.

Page 9 of 9 Ref: lpgen0007