Journal: Breast Cancer Research and Treatment

Supplementary material.

Journal: Breast Cancer Research and Treatment.

Title: Mutational analysis of telomere genes in BRCA1/2-negative breast cancer families with very short telomeres

Authors: Kira Yanowsky1,2, Alicia Barroso1,2, Ana Osorio1,2, Miguel Urioste1,2, Javier Benitez1,2, Beatriz

Martinez-Delgado1,2.

1 Human Genetics Group, Spanish National Cancer Research Centre (CNIO), Madrid 28029, Spain.2 Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Madrid 28029, Spain.

Corresponding Author:

Beatriz Martínez-Delgado. Human Genetics Group, Spanish National Cancer Research Centre (CNIO).

Melchor Fernandez Almagro 3, Madrid 28029, Spain. Phone: +34 912246900, Fax: +34 912246911. Email: [email protected]

1 GENE Forward (5’-3’) Reverse (5’-3’) TERF1 TERF1 1P GGCAAATGCGTTTTCATTTT GAACTGCTCCTCGTCGTTTC TERF1_E1 GTACCAAGCGAGCCATTTA ACCGGGATTCGAACAAAGTT TERF1_E2 TTTCCAACCAATGATTTTACACA CCCCTATGCACCAGACACTT TERF1_E3 AATCTCATCAACCTTTTTCTATTTG ATTAAGTTCCAGCCCCCAGT TERF1_E4 TGGGTCCTAAATGTGTGTGG AAGCATTTCTTTTCTCCTCCAA TERF1_E5 TTAAAATGAGGAGAGGCCAGA CCACACCTTTCTACAAAACCA Supplementary TERF1_E6 ACCACCTTCGTAACGCTGAT TCAGCAAGATAACATGCTACTCAAT Table. TERF1_E7 TGCTTATGTTTGGAGAAACTTG TGGGCTAACTAGGACCCTGA TERF1_E8 ACTAAGCAGGGAGAGCACCA GCAGTGTACCACCATAGCAAA List of primer TERF1_E9 CACTCCCCCTCTGATTTCAA AAAGATGGCAAAGGGCAAAT sequences used to TERF1_E10 TGGGCAGCTGAGCTTACATA CTGGTAGCACAACCCTCATT TERF2 amplify the telomere TERF2_E1 GGAACTACGGCGTCTGAGAA TCCCGGZTCTGTCTGAAGTC genes for mutational TERF2_E2 GCTGGAAGAGGCAGTCAATC GAAAAGCAGCCAAGACAACC screening. TERF2_E3 TGCATGCTCCATTTCCATAA CCGAATGGTGCAGTAACCTC TERF2_E4 CCGACGTCAGGTGATCCA GCTGCAGGTGTTACATGTTGA TERF2_E5 GGGCTTCCCTTCCTGTATGT CTGGCCTGCTTTTGCATT TERF2_E6 TGCAATTCTGTGGCCTCTTC GGGAGGCAAGGAGAGAGAAA TERF2_E7 TGCCTATGCTTGATTTGCAG CCACTCCTGCGTCAAGTTCT TERF2_E8 CAACGGGACAGGAAGGAGTA ACTTGAGCCCAGAAGGTTGA TERF2_E9 GTTTTCCCTTTGGGGACATT GATGTCCTGCCATTTGATCC TERF2_E10 CAAATGGCAGGACATCAAGA AGGAACCATGCTCCTGTGAA POT1 POT1_E1 CGTTGACGTCGCATCCT TCCCTTCACCCGCTAAATAA POT1_E2 TGGGTAAAACACTGGTTTTGTATTT GGAAAAAGCATTCTGAGTTATAGAAA POT1_E3 AAACTCCTTTGACTCCTCTTTG AAGGGCTATGAAGTAGAATACACACA POT1_E4 TTTTGGTGATTGATTCAAAAACTT TCAATCCCATCACTTTAGCTCAT POT1_E5&6 TTAGGCTGGAGATGGGTAAAA CACATGTATCTATGTGTGTGGCATA POT1_E7 GCTGTGTGCATTGCCTTAATTT GCATCAGTGTTGTTTGGCAAT POT1_E8 TGCTTTAAAATATTGAAAGTCAGG CAGTGAACAATACAGAGTTCTCTTCAA POT1_E9 TGGTTCGTAGGTTGTGCATC GGTGCTAACTTATAATTCCCAGTA POT1_E10 TTTTTCAAAAGGATCATAAAACTACTC AAAAATTGCTCTAACCCATTAAG POT1_E11 CCACTTTCTAAATAACAAATGATCT TCGGCTTAATCGATACCTTA POT1_E12 TCTGGATTTTGTGGGTAGAGC AGCAAGAACTAAACTGTCAATGTT POT1_E13 TGTGTGCTCTGTCACGTGTATT GCCAACATGGTGAAACTGTG POT1_E14 TTCTAAATCATGAATGTGGCATATC TGCCAACAAGACACGGTAGA POT1_E15 GCCTAGCGTAATATTTTCTTAGC CGTTTGTTTTATTATGGAAAATACTCA POT1_E16 TCACGCTTACACCAAAATCG GCAAAAGGAGTATTCTAACAAAACA POT1_E17 CATTTTATTTGCACTACTTGAAGG AGCACATGACCCCAGAACTT POT1_E18&19 GCAGTAAAAAGTTATAACAAACAAGC TCTGAATTGGCCAAACAACAT POT1_E20 GCAGACTCATATGGTTTGATCTTTT TCCTTTTAGGAACAAAGCAGGT POT1_E21 TTTTGGAGTTGAGACCAGCA GGGATTGTTAAAATATTCTTGCCTAC POT1_E22 CAGAGTTATTTATTTTGTTTTAATGG AAAACTCAGGTCAGGAAAAGA TPP1 TPP1_E1&2 CAAGGGAGGAGCCAGTACC GGGGCAGTCTGTGCTAAGTC TPP1_E3 CACCCTAACCAAAGCCATGT ATCCCACATCCTGTCCTCAG TPP1_E4 AAGCAATGAATGAGGGCAAG CACTGATGGGATGACTGGTG TPP1_E5&6 CCTCTGTTCTCTGACCTCCAG GGCTCTTTGCTTGGCTCAC TPP1_E7 TCTGCTCTGACTCCCTCCAT CCCACTGTCCAGTCCTCTTG TPP1_E8&9 TGACTATCTTTGTGCCCATCC CACAAGTGAAAGGTGGTGGTT TPP1_E10&11 TATCTTGGAGGTCCCCTTGG AGAGTTTGGAGATGGGCTGA TPP1_E12&13 CTACTTGCACCTTCACTCTTGT GGACAAGCCATCTCTCCTGA TINF2 TINF2_E1 CCTCTTACCGCCCTTTTCC CTTGTCAGGTGCTCGCATC TINF2_E2&3 AGACTAGTTGGAAAAGGTCAGC GGGCGACAGAGCAAGATTC TINF2_E4&5 CAAATGGCCAGGATTACAGG TTCTTATGCCCGGAGCCC TINF2_E6a GGCTCCGGGCATAAGAAAC TGAGGTGAGAGCAAGCAAAG TINF2_E6b CAGAGCAAAAGGAGTGAGTG TACAGTCACAGGAAGAAACAG TINF2_E6c GGAATCTCTGGAAAACTATCAG ACACCCAGATAATCTGGCAG

RAP1 RAP1_E1a CGTTAATTTCCAGGTCAAGGTG CCTCCAGCTCCAGCCTCT RAP1_E1b GTGTGCCGAGTGCAGGAG CCAGGAGAAAGATCCCAACC RAP1_E2 GGTTTATGTAAAAGAGGCTATTGCT CCAGTTTCAAAGAAGAATGAAGG RAP1_E3 TTGGTGATTAGGAGCTGGTTTT CCACCTCACCTACCATGACTT TERT TERT_E1 TTCCTTTCCGCGGCCC CGAAGGCCAGCACGTTC TERT_E2a GTGTCCTGCCTGAAGGAGCTG CGGATGGGTGGGAGTGGCGC TERT_E2b GCGCTGCCACTCAGGCCCGG GTAGGGGCACTGCGCGTGGTTCC TERT_E2c GTTTCTGGAGCTGCTTGGGA AGCCCCTACTGCATTCAGCT TERT_E3 GGGTTGGCTGTGTTCCGGCC TGGAGACAGCGCATGCTGA TERT_E4 CTTGTGGCTTCCCGTGAGCT CCCTTCATCTAAGCTGATACCA TERT_E5 TATGGTGGGGTGGGCACTTGG CTTGACAAAGTACAGCTCAGGCG TERT_E6 TGAGGGCAGAGGTGATGTCTG AGACACGACTGCATTCTAGACAC TERT_E7 CAGGTCCACACGTCCTGATCG ATGAGCCCAGTGATTGCCCAG TERT_E8 GAGGGTCTTGGCCACGTGGT GCAGGAGAGAGGTGAGCAGAAG TERT_E9 GTCCTACGTCCAGTGCCAGGG TCTTCAAGACAGAGCAGTCATG TERT_E10 GTTTGACATTCAGTCCCTCGTAG GCAGGACACGGGGGGCTCAA 2 TERT_E11 AGCATTTAACAAGGGTGTCAGGT GCAGAGGTGGACGCAACGGC TERT_E12 GGCAGGAGGCTCTTTGGAGC CTGCAAATCCAGAAACAGGCTGT TERT_E13 GTTCCTCTTTCTTGTTCCATCTG GGGTCAGAGGTGAGCAGAGC TERT_E14 GCCATGCCCACACCCACGA TTCACTCAGTCTCCTGACACAC TERT_E15 GAGGGGAGCTGGGCTGGGC AGGGCTGCTGGTGTCTGCTCTC TERT_E16 TCCTAGGAGGGTTGGAGGAT CCGTTCACTCACAGGCTTCT