JOURNAL OF BACTERIOLOGY

Volume 171 November 1989 No. 11

GENERAL MICROBIOLOGY Escherichia coli K-12 Cell-Cell Interactions Seen by Time-Lapse Video. James A. Shapiro and Clara Hsu ...... 5963-5974 Differential Activity of a Transposable Element in Escherichia coli Colonies. James A. Shapiro and N. Patrick Higgins...... 5975-5986 Calcium Requirement for Gliding Motility in Myxobacteria. Belinda J. Womack, David F. Gilmore, and David White ...... 6093-6096 Trehalose Accumulation in Vegetative Cells and Spores of Myxococcus xanthus. Mark J. McBride and David R. Zusman ...... 6383-6386

STRUCTURE AND FUNCTION Patterns of Cellular Interactions during Fruiting-Body Formation in Myxococcus xanthus. Kathleen A. O'Connor and David R. Zusman...... 6013-6024 Role of the CheW Protein in Bacterial Chemotaxis: Overexpression Is Equivalent to Absence. David Avram Sanders, Bernardita Mendez, and Daniel E. Koshland, Jr...... 6271-6278 Multiple Kinetic States for the Flagellar Motor Switch. Scot C. Kuo and Daniel E. Koshland, Jr...... 6279-6287 Cloning and Expression of Pseudomonas aeruginosa Flagellin in Eschenchia coli. Kimberly Kely-Wintenberg and Thomas C. Montie ...... 6357-6362 Structural Similarity among Escherichia coli FtsW and RodA Proteins and Bacil- lus subliUs SpoVE Protein, Which Function in Cell Division, Cell Elongation, and Spore Formation, Respectively. Masato Ikeda, Tsutomu Sato, Masaaki Wachi, Hai Kwan Jung, Fumitoshi Ishino, Yasuo Kobayashi, and Michio Matsuhashi...... 6375-6378

PLANT MICROBIOLOGY Altered Imino Diacid Synthesis and Transcription in Crown Gall Tumors with Transposon TnS Insertions in the 3' End of the Octopine Synthase Gene. Chi-cheng Chang, R. K. Jayaswal, Chong-Maw Chen, and Stanton B. Gelvin. 5922-5927 Genes Involved in Lipopolysaccharide Production and Symbiosis Are Clustered on the Chromosome of Rhizobium kguminosarum Biovar viciae VF39. Ursula B. Priefer ...... 6161-6168 Ti Plasmid-Controlled Chromosome Transfer in Agrobacterium tumefaciens. Y. Dessaux, A. Petit, J. G. Ellis, C. Legrain, M. Demarez, J.-M. Wiame, M. Popoff, and J. Tempe ...... 6363-6366

GENETICS AND MOLECULAR BIOLOGY Unique Organization of Leptospira interrogans rRNA Genes. Masahito Fukunaga and Ichiji Mifuchi ...... 5763-5767

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Identification and Analysis of Transcriptional Regulatory Signals for the kil and kor Loci of Streptomyces Plasmid pIj101. David S. Stein, Kevin J. Kendall, and Stanley N. Cohen...... 5768-5775 Cloning of Salmonella typhimurium DNA Encoding Mutagenic DNA Repair. Susan M. Thomas and Steven G. Sedgwick...... 5776-5782 Mechanism of Erythromycin-Induced ermC mRNA Stability in Bacillus subtilis. David H. Bechhofer and Kevin H. Zen...... 5803-5811 Translation Rates of Individual Codons Are Not Correlated with tRNA Abun- dances or with Frequencies of Utilization in Escherichia coli. Fons Bonekamp, Henrik Dalb0ge, Thorkild Christensen, and Kaj Frank Jensen 5812-5816 DNA Amplification in Streptomyces achromogenes subsp. rubradiris Is Accompa- nied by a Deletion, and the Amplified Sequences Are Conditionally Stable and Can Be Eliminated by Two Pathways. Ulfert Hornemann, Christopher J. Otto, and Xiao Yu Zhang...... 5817-5822 Physical and Genetic Mapping of the Rhodobacter sphaeroides 2.4.1 Genome: Genome Size, Fragment Identification, and Gene Localization. Antonius Suwanto and Samuel Kaplan...... 5840-5849 Physical and Genetic Mapping of the Rhodobacter sphaeroides 2.4.1 Genome: Presence of Two Unique Circular Chromosomes. Antonius Suwanto and Samuel Kaplan ...... 5850-5859 Fine-Structure Genetic Map of the Maltose Transport Operon of Salmonella typhimurium. Erwin Schneider, Lauren Bishop, Elke Schneider, Vivian Alfandary, and Giovanna Ferro-Luzzi Ames ...... 5860-5865 Cloning of Genes Governing the Deoxysugar Portion of the Erythromycin Bio- synthesis Pathway in Saccharopolyspora erythraea (Streptomyces erythreus). Jesus Vara, Marlena Lewandowska-Skarbek, Yi-guang Wang, Stefano Donadio, and C. Richard Hutchinson ...... 5872-5881 Sigma H-Directed Transcription of citG in Bacillus subtilis. Kathleen M. Tatti, H. Luke Carter III, Anne Moir, and Charles P. Moran, Jr...... 5928-5932 Role of Sigma H in Expression of the Fumarase Gene (citG) in Vegetative Cells of Bacillus subtilis 168. Vincent A. Price, Ian M. Feavers, and Anne Moir 5933-5939 Physical and Genetic Maps of the Genome of the Heterocyst-Forming Cyanobac- terium Anabaena sp. Strain PCC 7120. Ian Bancroft, C. Peter Wolk, and Elaine V. Oren ...... 5940-5948 Characterization of an Insertion Sequence (IS891) of Novel Structure from the Cyanobacterium Anabaena sp. Strain M-131. Ian Bancroft and C. Peter Wolk ...... 5949-5954 Regulation of Expression of the Pasteurella haemolytica Leukotoxin Determinant. Craig A. Strathdee and Reggie Y. C. Lo...... 5955-5962 The tyrT Locus of Escherichia coli Exhibits a Regulatory Function for Glycine Metabolism. Uwe Michelsen, Michael Bosl, Theodor Dingermann, and Helga Kersten...... 5987-5994 Participation of Escherichia coli Heat Shock Proteins DnaJ, DnaK, and GrpE in P1 Plasmid Replication. Kit Tilly and Michael Yarmolinsky...... 6025-6029 AdnaK52 Mutants of Escherichia coli Have Defects in Chromosome Segregation and Plasmid Maintenance at Normal Growth Temperatures. Bernd Bukau and Graham C. Walker...... 6030-6038 Molecular Cloning and Characterization of comC, a Late Competence Gene of Bacillus subtilis. Sandhya Mohan, Joel Aghion, Nancy Guillen, and David Dubnau...... 6043-6051

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groE Mutants of Escherichia coli Are Defective in umuDC-Dependent UV Muta- genesis. Caroline E. Donnelly and Graham C. Walker...... 6117-6125 Induction of the SOS Response by Hydrogen Peroxide in Various Escherichia coli Mutants with Altered Protection against Oxidative DNA Damage. Ottmar Goerlich, Philippe Quillardet, and Maurice Hofnung ...... 6141-6147 Evidence that the Myxococcus xanthus frz Genes Are Developmentally Regulated. Robin A. Weinberg and David R. Zusman...... 6174-6186 Characterization of the Gene for a Protein Kinase Which Phosphorylates the Sporulation-Regulatory Proteins SpoOA and SpoOF of Bacillus subtilis. Marta Perego, Sherilynn P. Cole, David Burbulys, Kathleen Trach, and James A. Hoch...... 6187-196 Characterization of the Cryptic Lambdoid Prophage DLP12 of Escherichia coli and Overlap of the DLI12 Integrase Gene with the tRNA Gene argU. David F. Lindsey, David A. Mullin, and James R. Walker ...... 6197-6205 Environmental Regulation of Expression of Virulence Determinants in BordeteUa pertussis. Angela R. Melton and Alison Ann Weiss ...... 6206-6212 Autogenous Regulation of the RNA Polymerase I Subunit of Escherchwa coli Occurs at the Translational Level In Vivo. Luciano Passador and Thomas Linn...... 6234-6242 Tandem Arrangement of Photolyase and Superoxide Dismutase Genes in Halobac- terium halobium. Masashi Takao, Takayasu Kobayashi, Atsushi Oikawa, and Akira Yasui ...... 6323-6329 Mutational Analysis of the Histidine Operon Promoter of Sabnonella typhimurium. Richard F. Shand, Paul H. Blum, Donald L. Holzschu, Mickey S. Urdea, and Stanley W. Artz...... 6330-6337 The bvgA Gene of Rordetella pertussis Encodes a Transcriptional Activator Required for Coordinate Regulation of Several Virulence Genes. Craig R. Roy, Jeff F. Miller, and Stanley Falkow ...... 6338-6344 Analysis of Bordetela pertussis Virulence Gene Regulation by Use of Transcrip- tional Fusions in Escherichia coli. Jeff F. Miller, Craig R. Roy, and Stanley Falkow...... 6345-6348 Molecular Cloning of the recA Analog from the Marine Fish Pathogen Vibrio anguillarum 775. John T. Singer...... 6367-6371 Inhibition of Growth offtsQ, ftsA, and ftsZ Mutant Cells of Escherichia coli by Amplification of a Chromosomal Region Encompassing Closely Aligned Cell Division and Cell Growth Genes. Hai Kwan Jung, Fumitoshi Ishino, and Michio Matsuhashi...... 6379-6382

PLASMIDS AND TRANSPOSONS Genetic Analysis of the Conjugal Transfer Determinants Encoded by the Strepto- coccal Broad-Host-Range Plasmid pIP501. E. Regis Krah III and Francis L. Macrina...... 6005-6012

EUCARYOTIC CELLS Na Acetylation Is Required for Normal Growth and Mating of Saccharomyces cerevisiae. Fang-jen S. Lee, Lee-wen Lin, and John A. Smith...... 5795-5802 Identification and Characterization of the Maltose Permease in a Genetically Defined Saccharomyces Strain. Young Sook Chang, Robert A. Dubin, Edward Perkins, Corinne A. Michels, and Richard B. Needleman...... 6148-6154

Continued on following page Continuedfrom preceding page Structural Discrimination in the Sparking Function of Sterols in the Yeast Saccharomyces cerevisiae. R. Todd Lorenz, Warren M. Casey, and Leo W. Parks...... 6169-6173 Oxidation of Neurospora crassa NADP-Specific Glutamate Dehydrogenase by Activated Oxygen Species. Jesus Aguirre, Rocio Rodriguez, and Wilhelm Hansberg. 6243-6250 Molecular Cloning of a Cell Wall Exo-(0-1,3-Glucanase from Saccharomyces cerevisiae. Franz Klebl and Widmar Tanner ...... 6259-6264 Isolation and Characterization of a Laccase-Derepressed Mutant of Neurospora crassa. Hisashi Tamaru and Hirokazu Inoue ...... 6288-6293 Arsenic Oxide-Induced Thermotolerance in Saccharomyces cerevisiae. Eric C. Chang, Daniel J. Kosman, and Gail R. Willsky...... 6349-6352 Characterization of the Inverted Duplication in the Mitochondrial DNA of Candida albicans. J. Andrew Shaw, W. Brad Troutman, Brent A. Lasker, Mark M. Mason, and W. Stuart Riggsby ...... 6353-6356 Effect of Glucose on Isocitrate Lyase in Phycomyces blakesleeanus. Javier Rua, Dolores de Arriaga, Felix Busto, and Joaquin Soler...... 6391-6393

CELL SURFACES Peptidoglycan Synthesis during the Cell Cycle of Escherichia coli: Composition and Mode of Insertion. Boudewijn L. M. de Jonge, Frans B. Wientjes, Inga Jurida, Frank Driehuis, Jan T. M. Wouters, and Nanne Nanninga .... 5783-5794 Construction, Expression, and Localization of a CycA::PhoA Fusion Protein in Rhodobacter sphaeroides and Escherichia coli. Amy R. Varga and Samuel Kaplan ...... 5830-5839 Genetic Analyses of Processing Involving C-Terminal Cleavage in Penicillin- Binding Protein 3 of Escherichia coli. Hiroshi Hara, Yukinobu Nishimura, Jun-ichi Kato, Hideho Suzuki, Hiromichi Nagasawa, Akinori Suzuki, and Yukinori Hirota...... 5882-5889 Determination of the Cleavage Site Involved in C-Terminal Processing of Penicil- lin-Binding Protein 3 of Escherichia coli. Hiromichi Nagasawa, Youji Sakagami, Akinori Suzuki, Hideho Suzuki, Hiroshi Hara, and Yukinori Hirota ...... 5890-5893 Export of FepA::PhoA Fusion Proteins to the Outer Membrane of Escherichia coli K-12. Christopher K. Murphy and Phillip E. Klebba...... 5894-5900 PapD, a Periplasmic Transport Protein in P-Pilus Biogenesis. Frederik Lindberg, Jan M. Tennent, Scott J. Hultgren, Bjorn Lund, and Staffan Normark . 6052-6058 Chemiosmotic Energy Conversion of the Archaebacterial Thermoacidophile Sulfolobus acidocaldarius: Oxidative Phosphorylation and the Presence of an F0-Related N,N'-Dicyclohexylcarbodiimide-Binding Proteolipid. Mathias Lubben and Gunter Schafer...... 6106-6116 Organization of the murE-murG Region of Escherichia coli: Identification of the murD Gene Encoding the D-Glutamic-Acid-Adding Enzyme. Dominique Mengin-Lecreulx, Claudine Parquet, Lourdes R. Desviat, Jesus Pla, Bernard Flouret, Juan A. Ayala, and Jean van Heijenoort...... 6126-6134 Peptide Uptake Is Essential for Growth of Lactococcus lactis on the Milk Protein Casein. Eddy J. Smid, R. Plapp, and Wil N. Konings...... 6135-6140

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Removal of CO Dehydrogenase from Pseudomonas carboxydovorans Cytoplasmic Membranes, Rebinding of CO Dehydrogenase to Depleted Membranes, and Restoration of Respiratory Activities. Susanne Jacobitz and Ortwin Meyer 6294-6299 The Acylated Precursor Form of the Colicin A Lysis Protein Is a Natural Substrate of the DegP Protease. Daniele Cavard, Claude Lazdunski, and S. Peter Howard...... 6316-6322 Purification and Analysis of Colonization Factor Antigen I, Coli Surface Antigen 1, and Coli Surface Antigen 3 Fimbriae from Enterotoxigenic Eschericha coli. Robert H. Hall, David R. Maneval, Jr., John H. Collins, Janet L. Theibert, and Myron M. Levine...... 6372-6374 The Structurally Related exbB and tolQ Genes Are Interchangeable in Conferring tonB-Dependent Colicin, Bacteriophage, and Albomycin Sensitivity. Volkmar Braun...... 6387-6390

PHYSIOLOGY AND METABOLISM Structure and Function of a Menaquinone Involved in Electron Transport in Membranes of Clostridium thermoautotrophicum and Clostridium thermoaceti- cum. Amaresh Das, Jeroen Hugenholtz, Herman van Halbeek, and Lars G. Ljungdahl...... 5823-5829 Transport of Coenzyme M (2-Mercaptoethanesulfonic Acid) and Methylcoenzyme M [(2-Methylthio)Ethanesulfonic Acid] in Methanococcus volae: Identifica- tion of Specific and General Uptake Systems. Michael Dybas and Jordan Konisky ...... 5866-5871 Cloning, Expression, and Regulation of the Pseudomonas cepacia Protocatechuate 3,4-Dioxygenase Genes. Gerben J. Zylstra, R. H. Olsen, and D. P. Ballou 5907-5914 Characterization of the Oxygen-Dependent Promoter of the Vitreoscilla Hemoglo- bin Gene in Escherichia coli. Chaitan Khosla and James E. Bailey...... 5995-6004 The Cytochrome bc, Complex of Rhodobacter sphaeroides Can Restore Cytochrome c2-Independent Photosynthetic Growth to a Rhodobacter capsulatus Mutant Lacking Cytochrome bcl. Edgar Davidson, Roger C. Prince, Copper E. Haith, and Fevzi Daldal ...... 6059-6068 The 5'-Flanking Region of the Gene Encoding the Large Subunit of Ribulose- 1,5-Bisphosphate Carboxylase/Oxygenase Is Crucial for Growth of the Cyanobacterium Synechococcus sp. Strain PCC 7942 at the Level of CO2 in Air. D. Friedberg, A. Kaplan, R. Ariel, M. Kessel, and J. Seijffers .... 6069-6076 Divergent Transcription of pdxB and Homology between the pdxB and serA Gene Products in Escherichia coli K-12. Patricia V. Schoenlein, Benjamin B. Roa, and Malcolm E. Winkler...... 6084-6092 Constitutive Activation of the fucAO Operon and Silencing of the Divergently Transcribed fucPIK Operon by an IS5 Element in Escherichia col Mutants Selected for Growth on L-1,2-Propanediol. Yu-Mei Chen, Zhe Lu, and E. C. C. Lin ...... 6097-6105 Anaerobic Fermentation Balance of Escherichia coli as Observed by In Vivo Nuclear Magnetic Resonance Spectroscopy. Kiswar Y. Alam and David P. Clark ...... 6213-6217 Molecular Cloning and Sequence Analysis of the Structural Gene of Ferredoxin I from the Photosynthetic Bacterium Rhodobacter capsulatus. Eric Schatt, Yves Jouanneau, and Paulette M. Vignais ...... 6218-6226

Continued on following page Continuedfrom preceding page Photosynthetic CO2 Fixation and Ribulose Bisphosphate Carboxylase/Oxygenase Activity of Nostoc sp. Strain UCD 7801 in Symbiotic Association with Antho- ceros punctatus. Nisan A. Steinberg and John C. Meeks ...... 6227-6233

ENZYMES AND PROTEINS Genetic Organization and Sequence of the Pseudomonas cepacia Genes for the Alpha and Beta Subunits of Protocatechuate 3,4-Dioxygenase. Gerben J. Zylstra, R. H. Olsen, and D. P. Ballou...... 5915-5921 Purification and Some Properties of Glutathione S-Transferase from Escherichia coli B. Masaki lizuka, Yoshiharu Inoue, Kousaku Murata, and Akira Kimura ...... 6039-6042 Phosphatidylinositol-Specific Phospholipase C of Bacillus cereus: Cloning, Sequencing, and Relationship to Other Phospholipases. Andreas Kuppe, Loreene M. Evans, Debra A. McMillen, and 0. Hayes Griffith ...... 6077-6083 Is the First Enzyme of the Shikimate Pathway, 3-Deoxy-D-arabino-Heptulosonate- 7-Phosphate Synthase (Tyrosine Sensitive), a Copper Metalioenzyme? Timor Baasov and Jeremy R. Knowles...... 6155-6160 Physically Associated Enzymes Produce and Metabolize 2-Hydroxy-2,4-Dienoate, a Chemically Unstable Intermediate Formed in Catechol Metabolism via meta Cleavage in Pseudomonas putida. Shigeaki Harayama, Monique Rekik, Ka-Leung Ngai, and L. Nicholas Ornston ...... 6251-6258 Purification of a Fourth Glucosyltransferase from Streptococcus sobrinus. Yoshihisa Yamashita, Nobuhiro Hanada, and Tadamichi Takehara...... 6265-6270 Cloning and Nucleotide Sequence of braC, the Structural Gene for the Leucine-, Isoleucine-, and Valine-Binding Protein of Pseudomonas aeruginosa PAO. Toshimitsu Hoshino and Kumiko Kose...... 6300-6306 S-Layer Protein Gene of Acetogenium kivui: Cloning and Expression in Esche- richia coli and Determination of the Nucleotide Sequence. Jurgen Peters, Marietta Peters, Friedrich Lottspeich, and Wolfgang Baumeister...... 6307-6315

POPULATION GENETICS AND EVOLUTION 16S rRNA Sequence Indicates that Plant-Pathogenic Mycoplasmalike Organisms Are Evolutionarily Distinct from Animal Mycoplasmas. Pyung-Ok Lim and Barbara B. Sears ...... 5901-5906

ERRATUM Molecular Analysis and Regulation of the g1nA Gene of the Gram-Positive Anaer- obe Clostridium acetobutylicum. Paul J. Janssen, Winsome A. Jones, David T. Jones, and David R. Woods ...... 6394

AUTHOR'S CORRECTION Multiple Chromosomes of Azotobacter vinelandii. Punita Nagpal, Samina Jafri, M. A. Reddy, and H. K. Das ...... 6395

Date of Issue: 30 October 1989