A Biosurfactant Produced By Fermentation

Leading the future through an industry changing technology The Power of D-Phase

Peptide ring consists KANEKA Surfactin is able to create D-phase, emulsion, a of 77 aminoamino acidsacids bi-continuous phase that incorporates both oil and , using very low dosage. The D-phase KANEKA Surfactin creates could be used to formulate make up remover, an emulsion with very re ned oil droplets and many other skincare products.

※D-phase refers to the phase that consists of , polyol.

Clear Oil Gel Formulation Example KANEKA Surfactin 0.5%~1% Glycerin 20% Oil 70%~80% Water Adjusted according to type of oil

D-Phase emulsion created using KANEKA D-Phase Emulsion Surfactin contains large percentage of oil yet remains thin and smooth Extremely stable emulsion with average oil droplet size of 1 micron Merely 0.1% of KANEKA Surfactin is used to emulsify 10% of oil The extremely low dosage of emulsi er, KANEKA Surfactin, leads to no tackiness on skin ※skin touch may dier between individuals.

Bene t As A Co-

Helps to decrease usage of ANIONIC surfactants Helps to stabilize emulsions when used with NONIONIC Emulsion of soybean oil and water (25℃ 1 day) surfactants

LAS Emulsi cation result of 20% squalane (0.002wt%) GLYCERYL STEARATE SE 1.8%/ SF +SF Below CMC of LAS GLYCERYL STEARATE SE 2% No Surfactant (0.0005wt%) (0.0005wt%) (0.002wt%) KANEKA Surfactin 0.2% Frequency (%) Cumulative (%) Frequency (%) Cumulative (%)

Particle size (μm) Particle size (μm)

Adding 10mol% of SF (with respect to LAS) helps to emulsify Red:Immediately after preparation Green:50℃ for 8 weeks ※SF: KANEKA Surfactin Product Characteristics

Natural surfactant with high biodegradability and low skin irritation Highly ecient with critical of 0.0003% An uncommon chemical structure contributes to unique, distinguishing properties

Manufacturing Process

Produced by natural fermentation

Fermentation Puri cation

KANEKA Surfactin subtilis KANEKA Surfactin

Sustainability

Chemical Structure

L-Leu

D - Leu Alkyl Chain (Hydrophobic)

L- Glu Ring (Hydrophilic) KANEKA Surfactin has a peptide ring, which consists of 7 amino , L- Asp as its hydrophilic head and an alkyl chain as its hydrophobic tail. Its L- Leu unique chemical structure brings about many unique properties that L - Val are dierent from typical surfactants. Moreover, it is a mild, safe and + 3/2 Na+ environmentally friendly product. D -Leu + 1/2 H+

Primary Skin Irritation Test Biodegradation Test

8 100 Concentration River (Kakogawa, Hyogo, Japan)

80 Sea(Akashi, Hyogo, Japan) 6 2.5% 5% 10% 20% Substance: 100mg/L 60 Sludge: 10wt% Temperature: 25°C 4 40 No observable irritation Biodegrades within 1 week with 2.5% or below 2 20 Primary Irritation Index (P.I.I.) 0 36 KANEKA Surfactin Survival Rate (%) 0 KANEKA Surfactin Sodium Lauroyl SDS 0 2 4 6 8 Glutamate 40 Days PRODUCT INFORMATION

Product Name KANEKA Surfactin

INCI name: Sodium Surfactin

CAS No.: 302933-83-1

Purity: ＞90％

Appearance: White to whitish powder

Package： Carton 1kg/5kg

Origin： Japan

REFERENCE INFORMATION

Surface Active Property: Critical Micelle Concentration: 0.0003 wt% (Wilhelmy plate method @25℃)

Heat Stability: 1%weight decrease temp.：232℃ (powder in air)

Soluble in aqueous solvents. Precipitation at acidic pH or with Solubility: alkaline earth metal (M g2+, C a2+)

pＨ6.5 ～ 8.0 Recommended pH: (Precipitation at acidic pH. Hydrolysis at pH＞10)

Regulation: REACH (EU), TSCA (US), IECSC/IECIC (CN), NZIoC（NZ）

Certificate: COSMOS、Ecocert Others Used in Cosmetics, Quasi-Drug

Contact Information:

Kaneka Corporation New Business Development Department 2-3-18, Nakanoshima, Kita-ku, Osaka, 530-8288, Japan E-Mail: [email protected]