Submitted from PROJ13880 Lingzhu by Mascot Daemon on APAF-WS-08)

Peptide Summary Report (Submitted from PROJ13880 LingZhu by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120608/F077324.dat&REPTYPE=pe...

User : Matt F Email : Search title : Submitted from PROJ13880 LingZhu by Mascot Daemon on APAF-WS-08 MS data file : \\Apaf-hpv-file\User_Shared\MFitzhenry\2012 Reports\PROJ13880_LingZhu(SSI)\A6_mgf.mgf Database : SwissProt 2012x (536029 sequences; 190235160 residues) Taxonomy : Homo sapiens (human) (20319 sequences) Timestamp : 8 Jun 2012 at 00:50:07 GMT Protein hits : RET3_HUMAN Retinol-binding protein 3 OS=Homo sapiens GN=RBP3 PE=1 SV=2 SPTA2_HUMAN Spectrin alpha chain, brain OS=Homo sapiens GN=SPTAN1 PE=1 SV=3 K2C1_HUMAN Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 K1C10_HUMAN Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 K22E_HUMAN Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2 K1C9_HUMAN Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 CERU_HUMAN Ceruloplasmin OS=Homo sapiens GN=CP PE=1 SV=1 ALBU_HUMAN Serum albumin OS=Homo sapiens GN=ALB PE=1 SV=2 VIME_HUMAN Vimentin OS=Homo sapiens GN=VIM PE=1 SV=4 MED12_HUMAN Mediator of RNA polymerase II transcription subunit 12 OS=Homo sapiens GN=MED12 PE=1 SV=4 H6ST2_HUMAN Heparan-sulfate 6-O-sulfotransferase 2 OS=Homo sapiens GN=HS6ST2 PE=2 SV=2 CAPSD_HASV1 Capsid polyprotein OS=Human astrovirus-1 GN=ORF2 PE=3 SV=1 BIRC6_HUMAN Baculoviral IAP repeat-containing protein 6 OS=Homo sapiens GN=BIRC6 PE=1 SV=2 PNMA2_HUMAN Paraneoplastic antigen Ma2 OS=Homo sapiens GN=PNMA2 PE=1 SV=2 WDFY3_HUMAN WD repeat and FYVE domain-containing protein 3 OS=Homo sapiens GN=WDFY3 PE=1 SV=2 PTOV1_HUMAN Prostate tumor-overexpressed gene 1 protein OS=Homo sapiens GN=PTOV1 PE=1 SV=1

Mascot Score Histogram

Ions score is -10*Log(P), where P is the probability that the observed match is a random event. Individual ions scores > 30 indicate identity or extensive homology (p<0.05). Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.

Peptide Summary Report

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Downloaded from iovs.arvojournals.org on 09/26/2021 1 of 23 8/06/2012 1:09 PM Peptide Summary Report (Submitted from PROJ13880 LingZhu by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120608/F077324.dat&REPTYPE=pe...

Significance threshold p< Max. number of hits Standard scoring MudPIT scoring Ions score or expect cut-off Show sub-sets Show pop-ups Suppress pop-ups Sort unassigned Require bold red

Error tolerant

1. RET3_HUMAN Mass: 135278 Score: 2746 Matches: 83(61) Sequences: 45(43) emPAI: 2.84 Retinol-binding protein 3 OS=Homo sapiens GN=RBP3 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 54 420.2038 838.3931 838.5025 -130.43 0 (4) 22 1 U K.AVGPQLVR.L 55 420.2562 838.4978 838.5025 -5.53 0 48 0.00083 1 U K.AVGPQLVR.L 79 430.2289 858.4432 858.4559 -14.84 0 (9) 9.7 1 U R.TGGGALDLR.K 80 430.2289 858.4432 858.4559 -14.84 0 83 4.1e-007 1 U R.TGGGALDLR.K 87 433.7229 865.4312 865.4294 2.15 0 39 0.0078 1 U R.SPGLQDHL.- 94 435.7862 869.5579 869.5698 -13.75 0 36 0.014 1 U K.ALAILTLR.S 95 435.7902 869.5658 869.5698 -4.66 0 (15) 1.7 1 U K.ALAILTLR.S 126 450.2701 898.5256 898.5348 -10.31 1 34 0.023 1 U R.RAIVVGER.T 178 475.7480 949.4814 949.4981 -17.60 0 37 0.014 1 U K.AAHIPENAK.D 202 489.2802 976.5458 976.5454 0.42 1 29 0.098 1 U R.AKLAQGAYR.T 209 494.2838 986.5530 986.5509 2.15 1 59 7.9e-005 1 U R.TGGGALDLRK.L 235 507.3036 1012.5927 1012.5917 0.94 0 65 1.8e-005 1 U K.VPTVLQTAGK.L 246 511.7710 1021.5275 1021.5305 -2.89 1 42 0.0039 1 U K.RSPGLQDHL.- 264 519.3128 1036.6110 1036.6069 3.88 0 46 0.0015 1 U R.LLVEHIWK.K 274 523.2287 1044.4429 1044.5716 -123.25 0 (7) 12 2 U R.GVYLLTSHR.T 275 523.2525 1044.4905 1044.5716 -77.68 0 (3) 31 3 U R.GVYLLTSHR.T 276 523.2547 1044.4948 1044.5716 -73.53 0 (15) 2 1 U R.GVYLLTSHR.T 277 523.2742 1044.5338 1044.5716 -36.24 0 (10) 6.6 1 U R.GVYLLTSHR.T 279 523.2915 1044.5684 1044.5716 -3.09 0 39 0.0084 1 U R.GVYLLTSHR.T 362 583.2853 1164.5561 1164.6503 -80.82 0 (5) 21 1 U R.GVAEDIAHILK.Q 363 583.2853 1164.5561 1164.6503 -80.82 0 (7) 11 1 U R.GVAEDIAHILK.Q 365 583.2945 1164.5744 1164.6503 -65.13 0 (17) 1.1 1 U R.GVAEDIAHILK.Q 366 583.3173 1164.6201 1164.6503 -25.89 0 (5) 21 1 U R.GVAEDIAHILK.Q 367 583.3287 1164.6429 1164.6503 -6.27 0 66 1.5e-005 1 U R.GVAEDIAHILK.Q 368 583.3539 1164.6932 1164.7019 -7.45 1 19 0.71 1 U R.LLVEHIWKK.I 404 407.8822 1220.6248 1220.6261 -1.12 1 43 0.0034 1 U K.AAHIPENAKDR.I 427 627.3102 1252.6058 1252.6009 3.89 0 (68) 9.7e-006 1 U R.FDAMAELETVK.A 437 635.2992 1268.5839 1268.5958 -9.40 0 (20) 0.59 1 U R.FDAMAELETVK.A + Oxidation (M) 438 635.3016 1268.5887 1268.5958 -5.64 0 (40) 0.0058 1 U R.FDAMAELETVK.A + Oxidation (M) 439 635.3040 1268.5935 1268.5958 -1.88 0 (20) 0.52 1 U R.FDAMAELETVK.A + Oxidation (M) 440 635.3040 1268.5935 1268.5958 -1.88 0 (12) 4.1 1 U R.FDAMAELETVK.A + Oxidation (M) 441 635.3040 1268.5935 1268.5958 -1.88 0 (42) 0.004 1 U R.FDAMAELETVK.A + Oxidation (M) 442 635.3088 1268.6030 1268.5958 5.64 0 79 7.6e-007 1 U R.FDAMAELETVK.A + Oxidation (M)

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443 635.3159 1268.6173 1268.5958 16.9 0 (19) 0.71 1 U R.FDAMAELETVK.A + Oxidation (M) 483 671.3472 1340.6799 1340.6684 8.57 0 93 2.8e-008 1 U K.LNAGLQAASEDPR.L 484 671.3546 1340.6946 1340.6684 19.5 0 (7) 13 1 U K.LNAGLQAASEDPR.L 486 673.3686 1344.7226 1344.7401 -13.07 0 (44) 0.0024 1 U K.TEVLPGQLGYLR.F 487 673.3710 1344.7275 1344.7401 -9.42 0 94 2.3e-008 1 U K.TEVLPGQLGYLR.F 488 673.3735 1344.7324 1344.7401 -5.77 0 (41) 0.0047 1 U K.TEVLPGQLGYLR.F 489 673.3784 1344.7422 1344.7401 1.53 0 (33) 0.028 1 U K.TEVLPGQLGYLR.F 490 673.3808 1344.7471 1344.7401 5.18 0 (31) 0.05 1 U K.TEVLPGQLGYLR.F 491 673.3808 1344.7471 1344.7401 5.18 0 (68) 9.5e-006 1 U K.TEVLPGQLGYLR.F 506 461.9093 1382.7060 1382.7088 -2.03 1 56 0.00016 1 U R.AKEMLQHNQLR.V + Oxidation (M) 509 462.5785 1384.7136 1384.7099 2.65 0 66 1.4e-005 1 U R.HEVLEGNVGYLR.V 523 711.3339 1420.6533 1420.7198 -46.80 0 (40) 0.0055 1 U K.LVADNYASAELGAK.M 524 711.3440 1420.6735 1420.7198 -32.59 0 (27) 0.1 1 U K.LVADNYASAELGAK.M 525 711.3541 1420.6937 1420.7198 -18.38 0 (60) 4.9e-005 1 U K.LVADNYASAELGAK.M 526 711.3617 1420.7088 1420.7198 -7.72 0 99 6.9e-009 1 U K.LVADNYASAELGAK.M 527 711.3667 1420.7189 1420.7198 -0.62 0 (95) 1.8e-008 1 U K.LVADNYASAELGAK.M 528 711.3718 1420.7290 1420.7198 6.48 0 (68) 8.6e-006 1 U K.LVADNYASAELGAK.M 552 493.5626 1477.6660 1477.6727 -4.54 0 (41) 0.0038 1 U K.IMHTDAMIIDMR.F + 2 Oxidation (M) 553 493.5647 1477.6723 1477.6727 -0.27 0 63 2.9e-005 1 U K.IMHTDAMIIDMR.F + 2 Oxidation (M) 578 792.4346 1582.8547 1582.8468 5.01 0 90 4.7e-008 1 U K.VTEVWTLPQVAGQR.Y 587 800.9046 1599.7946 1599.7933 0.78 0 100 5.1e-009 1 U R.IGESDFFFTVPVSR.S 588 800.9046 1599.7946 1599.7933 0.78 0 (90) 5.1e-008 1 U R.IGESDFFFTVPVSR.S 590 536.2582 1605.7527 1605.7677 -9.36 1 (26) 0.14 1 U K.KIMHTDAMIIDMR.F + 2 Oxidation (M) 595 541.5971 1621.7696 1621.7626 4.32 1 53 0.00027 1 U K.KIMHTDAMIIDMR.F + 3 Oxidation (M) 628 571.3110 1710.9113 1710.9165 -3.06 1 98 8.2e-009 1 U R.GLRHEVLEGNVGYLR.V 631 857.9155 1713.8164 1713.8032 7.69 0 127 1.1e-011 1 U R.FDMFGDGELLTQVSR.L 634 865.9033 1729.7921 1729.7982 -3.52 0 (122) 2.9e-011 1 U R.FDMFGDGELLTQVSR.L + Oxidation (M) 636 580.3006 1737.8801 1737.8897 -5.57 1 (82) 2.9e-007 1 U R.YGADKDVVVLTSSQTR.G 637 870.1985 1738.3825 1737.8897 284 1 101 4.8e-009 1 U R.YGADKDVVVLTSSQTR.G 666 624.0044 1868.9914 1868.9785 6.88 1 48 0.0007 1 U K.LRIGESDFFFTVPVSR.S 671 642.3218 1923.9437 1923.9479 -2.19 0 (20) 0.44 1 U K.FSFHTNVLEDNIGYLR.F 672 642.3266 1923.9581 1923.9479 5.29 0 (6) 13 2 U K.FSFHTNVLEDNIGYLR.F 673 642.3266 1923.9581 1923.9479 5.29 0 84 1.8e-007 1 U K.FSFHTNVLEDNIGYLR.F 681 651.7164 1952.1273 1952.1095 9.12 0 109 5.9e-010 1 U R.LVWQQLVDTAALVIDLR.Y 698 1020.5418 2039.0691 2039.0728 -1.83 0 127 8.4e-012 1 U R.FDSFADASVLGVLAPYVLR.Q 719 708.3647 2122.0724 2122.0517 9.73 0 63 2.1e-005 1 U R.QVWEPLQDTEHLIMDLR.H 722 713.6950 2138.0633 2138.0466 7.79 0 (56) 0.00012 1 U R.QVWEPLQDTEHLIMDLR.H + Oxidation (M) 736 1118.6102 2235.2059 2235.2225 -7.40 0 (53) 0.00019 1 U R.IPGIVPMQIPSPEVFEELIK.F 738 1126.6075 2251.2004 2251.2174 -7.53 0 59 4.8e-005 1 U R.IPGIVPMQIPSPEVFEELIK.F + Oxidation (M) 739 754.4093 2260.2059 2260.2216 -6.92 0 80 3.5e-007 1 U R.QALVDSVFQVSVLPGNVGYLR.F 759 841.7929 2522.3567 2522.3454 4.48 1 63 1.9e-005 1 U K.DRIPGIVPMQIPSPEVFEELIK.F + Oxidation (M) 762 636.8191 2543.2472 2543.2405 2.65 0 57 6.6e-005 1 U K.IYSRPDDSVSELWTHAQVVGER.Y 765 852.4267 2554.2582 2554.2511 2.80 0 136 1.1e-012 1 U R.TAVDLESLASQLTADLQEVSGDHR.L 775 875.1255 2622.3548 2622.3575 -1.01 0 100 3.9e-009 1 U R.VTSEVALAEILGADLQMLSGDPHLK.A + Oxidation (M) 782 925.8026 2774.3859 2774.4048 -6.81 0 109 5.3e-010 1 U R.VPTLLQHLASMDFSTVVSEEDLVTK.L + Oxidation (M)

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784 955.1477 2862.4214 2862.4148 2.28 0 61 3.3e-005 1 U R.SVGASDGSSWEGVGVTPHVVVPAEEALAR.A 789 732.1001 2924.3714 2924.3433 9.63 0 43 0.0018 1 U K.DLYILMSHTSGSAAEAFAHTMQDLQR.A + 2 Oxidation (M) 793 1012.5306 3034.5699 3034.5571 4.23 0 147 9.2e-014 1 U K.SHEILSISDPQTLASVLTAGVQSSLNDPR.L 804 1152.8828 3455.6266 3455.6368 -2.96 0 80 5.7e-007 1 U R.AIGPTETPSWPAPDAAAEDSPGVAPELPEDEAIR.Q 808 1350.3303 4047.9690 4047.9479 5.20 0 156 1.4e-014 1 U R.ATVIGEPTAGGALSVGIYQVGSSPLYASMPTQMAMSATTGK.A + 3 Oxidation (M)

2. SPTA2_HUMAN Mass: 284364 Score: 2225 Matches: 72(63) Sequences: 70(62) emPAI: 1.33 Spectrin alpha chain, brain OS=Homo sapiens GN=SPTAN1 PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 22 402.7398 803.4651 803.4654 -0.38 0 23 0.44 1 U R.LQQLFR.D 32 407.7160 813.4174 813.4167 0.92 0 8 11 1 U R.LMELHR.Q + Oxidation (M) 76 429.2385 856.4624 856.4629 -0.56 1 29 0.083 1 U K.KFMLFR.E + Oxidation (M) 96 436.7703 871.5260 871.4341 105 0 23 0.39 1 U R.FQFFQR.D 105 441.2421 880.4696 880.4766 -8.01 0 18 0.91 1 U K.LHELNQK.W 123 449.7884 897.5623 897.5760 -15.27 1 39 0.0072 1 U R.LKGLALQR.Q 142 458.7712 915.5279 915.5277 0.31 0 49 0.00084 1 U K.LLEATELK.G 173 473.7650 945.5154 945.5131 2.45 0 67 1.3e-005 1 U K.LTVLSEER.A 208 493.7812 985.5479 985.5556 -7.86 0 45 0.0023 1 U R.IDGITIQAR.Q 213 495.7677 989.5209 989.5294 -8.65 0 76 2e-006 1 U K.LFGAAEVQR.F 216 497.2855 992.5565 992.5655 -9.07 1 37 0.014 1 U R.FKELSTLR.R 272 522.8155 1043.6164 1043.6226 -5.96 1 73 3.5e-006 1 U K.KLLEATELK.G 287 529.7974 1057.5803 1057.5808 -0.41 0 58 0.00011 1 U R.QWELLLEK.M 303 536.7885 1071.5624 1071.5672 -4.55 1 32 0.053 1 U K.KLDPAQSASR.E 307 540.2902 1078.5658 1078.5811 -14.25 0 54 0.00024 1 U R.QGFVPAAYVK.K 312 543.2144 1084.4142 1084.6240 -193.49 0 (1) 56 4 U R.DLASVQALLR.K 314 543.3224 1084.6303 1084.6240 5.74 0 59 8.9e-005 1 U R.DLASVQALLR.K 323 551.3077 1100.6009 1100.6077 -6.18 1 45 0.0023 1 U R.DLAALEDKVK.A 333 556.8370 1111.6595 1111.6601 -0.57 0 50 0.00055 1 U K.DLIGVQNLLK.K 338 560.2869 1118.5593 1118.5720 -11.33 0 87 1.3e-007 1 U R.VNEVNQFAAK.L 347 568.2940 1134.5735 1134.5743 -0.72 0 71 5.8e-006 1 U R.MNEVISLWK.K + Oxidation (M) 368 583.3539 1164.6932 1164.6404 45.4 1 4 24 9 U R.LQRFLADFR.D 373 586.8184 1171.6223 1171.6197 2.22 0 38 0.0095 1 U K.DLTNVQNLQK.K 392 603.3070 1204.5994 1204.5976 1.55 0 54 0.00026 1 U R.DVDETISWIK.E 396 605.2931 1208.5716 1208.5747 -2.61 0 (31) 0.043 1 U R.DLTSWVTEMK.A 400 607.3453 1212.6761 1212.6866 -8.65 0 71 4.9e-006 1 U R.AALLELWELR.R 402 405.8842 1214.6308 1214.6255 4.42 1 34 0.029 1 U R.EKEPIAASTNR.G 407 613.2910 1224.5675 1224.5696 -1.71 0 69 7.4e-006 1 U R.DLTSWVTEMK.A + Oxidation (M) 454 646.2961 1290.5776 1290.5728 3.73 0 76 1.4e-006 1 U R.DVEDEETWIR.E 461 649.3975 1296.7805 1296.7765 3.04 1 58 8.7e-005 1 U R.GKDLIGVQNLLK.K 462 651.8371 1301.6597 1301.6463 10.3 0 94 2.3e-008 1 U K.VLETAEDIQER.R 473 441.5601 1321.6584 1321.6700 -8.75 1 35 0.017 1 U R.YEALKEPMVAR.K + Oxidation (M) 496 684.7853 1367.5559 1367.5663 -7.60 0 82 3.6e-007 1 U R.DTEQVDNWMSK.Q + Oxidation (M)

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497 685.3993 1368.7841 1368.7877 -2.62 1 41 0.0047 1 U R.AALLELWELRR.Q 534 715.8626 1429.7107 1429.7201 -6.58 0 67 1.1e-005 1 U R.EELITNWEQIR.T 538 482.5756 1444.7048 1444.7059 -0.72 1 58 9.7e-005 1 U K.TKQDEVNAAWQR.L 541 725.3427 1448.6709 1448.6783 -5.10 1 46 0.0015 1 U R.DSDELKSWVNEK.M 544 486.9267 1457.7584 1457.7474 7.52 1 53 0.00026 1 U K.VLETAEDIQERR.Q 548 736.3914 1470.7682 1470.7678 0.32 0 98 9.7e-009 1 U R.ENLLEEQGSIALR.Q 557 751.3618 1500.7090 1500.7208 -7.89 0 97 1e-008 1 U R.EANELQQWINEK.E 563 759.3778 1516.7411 1516.7409 0.16 1 80 4.8e-007 1 U R.DAEELEKWIQEK.L 566 511.2618 1530.7636 1530.7651 -0.98 1 52 0.00033 1 U K.LREANQQQQFNR.N 571 519.6060 1555.7962 1555.7855 6.84 0 105 1.8e-009 1 U K.HQALQAEIAGHEPR.I 579 529.6211 1585.8414 1585.8212 12.7 1 44 0.0024 1 U K.REELITNWEQIR.T 580 529.9216 1586.7429 1586.7437 -0.52 0 61 4.1e-005 1 U K.HQAFEAELSANQSR.I 589 535.9515 1604.8327 1604.8409 -5.16 0 32 0.029 1 U K.LIQEQHPEEELIK.T 596 541.6082 1621.8026 1621.8100 -4.54 1 29 0.063 1 U R.FNRDVDETISWIK.E 621 559.9802 1676.9188 1676.9210 -1.31 0 72 3.6e-006 1 U R.LQQSHPLSATQIQVK.R 623 421.9782 1683.8837 1683.8805 1.94 1 33 0.027 1 U K.KHQALQAEIAGHEPR.I 627 569.9641 1706.8706 1706.8529 10.4 0 37 0.012 1 U R.AQLADSFHLQQFFR.D 630 429.2287 1712.8857 1712.8846 0.66 1 26 0.12 1 U K.LIDVNHYAKDEVAAR.M 633 573.9303 1718.7690 1718.7570 7.01 0 70 5.6e-006 1 U K.GNAMVEEGHFAAEDVK.A + Oxidation (M) 635 579.9474 1736.8205 1736.8217 -0.68 1 68 7.9e-006 1 U K.TATDEAYKDPSNLQGK.V 639 583.2899 1746.8479 1746.8325 8.79 1 37 0.0099 1 U R.QFQDAGHFDAENIKK.K 654 596.3237 1785.9493 1785.9148 19.3 0 32 0.035 1 U K.QVEELYHSLLELGEK.R 655 597.6204 1789.8395 1789.8417 -1.25 0 85 1.4e-007 1 U K.LIQNNHYAMEDVATR.R + Oxidation (M) 661 600.3062 1797.8968 1797.9009 -2.33 0 93 2.5e-008 1 U K.HQAFEAEVQANSGAIVK.L 668 628.6212 1882.8417 1882.8156 13.9 1 93 2.3e-008 1 U K.KFEEFQTDMAAHEER.V + Oxidation (M) 670 640.3142 1917.9207 1917.8891 16.5 1 70 4.6e-006 1 U K.GNAMVEEGHFAAEDVKAK.L + Oxidation (M) 679 487.4905 1945.9330 1945.9428 -5.02 1 35 0.016 1 U K.LIQNNHYAMEDVATRR.D + Oxidation (M) 687 658.9940 1973.9602 1973.9557 2.30 1 30 0.042 1 U R.FLADFRDLTSWVTEMK.A + Oxidation (M) 689 661.0137 1980.0193 1980.0051 7.17 1 100 5.1e-009 1 U K.SLSAQEEKITALDEFATK.L 697 678.6816 2033.0230 2033.0065 8.11 1 52 0.00026 1 U K.LQIASDENYKDPTNLQGK.L 721 709.6973 2126.0701 2126.0855 -7.21 0 126 1.1e-011 1 U K.ALINADELASDVAGAEALLDR.H 724 716.3386 2145.9939 2145.9927 0.57 0 99 5.3e-009 1 U K.SADESGQALLAAGHYASDEVR.E 732 549.0266 2192.0773 2192.0895 -5.58 1 70 3.8e-006 1 U K.KHEALMSDLSAYGSSIQALR.E + Oxidation (M) 735 746.0267 2235.0583 2235.0477 4.72 0 87 8.4e-008 1 U K.LDETGNLMISEGHFASETIR.T + Oxidation (M) 749 784.3859 2350.1358 2350.1587 -9.72 1 40 0.0034 1 U K.EQLMASDDFGRDLASVQALLR.K + Oxidation (M) 751 789.4149 2365.2227 2365.2013 9.08 0 77 7.4e-007 1 U K.EAIVTSEELGQDLEHVEVLQK.K 754 601.7929 2403.1425 2403.1302 5.11 1 70 3.9e-006 1 U K.SADESGQALLAAGHYASDEVREK.L 756 1210.1106 2418.2066 2418.2166 -4.11 0 140 3.7e-013 1 U K.QEAFLLNEDLGDSLDSVEALLK.K 779 918.4626 2752.3659 2752.3443 7.85 1 83 1.8e-007 1 U R.QQVAPTDDETGKELVLALYDYQEK.S

3. K2C1_HUMAN Mass: 65999 Score: 478 Matches: 14(13) Sequences: 14(13) emPAI: 1.15 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 Check to include this hit in error tolerant search or archive report

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Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 46 416.7494 831.4842 831.4814 3.32 0 30 0.081 1 U K.SISISVAR.G 99 437.7455 873.4765 873.4920 -17.70 0 52 0.00052 1 U R.SLVNLGGSK.S 197 487.2733 972.5321 972.5240 8.36 0 61 5.1e-005 1 K.IEISELNR.V 257 517.2559 1032.4973 1032.5087 -11.06 0 52 0.0004 1 U R.TLLEGEESR.M 293 533.2588 1064.5031 1064.5138 -10.07 0 53 0.00033 1 U K.AQYEDIAQK.S 379 590.3070 1178.5995 1178.5931 5.37 0 82 4.2e-007 1 K.YEELQITAGR.H 463 651.8589 1301.7032 1301.7078 -3.55 0 79 6.9e-007 1 U R.SLDLDSIIAEVK.A 477 666.7590 1331.5035 1331.5122 -6.50 0 37 0.014 1 U K.NMQDMVEDYR.N + 2 Oxidation (M) 481 447.5641 1339.6704 1339.6619 6.33 1 38 0.0098 1 U K.SKAEAESLYQSK.Y 504 692.3294 1382.6442 1382.6830 -28.07 0 72 3.5e-006 1 U K.SLNNQFASFIDK.V 512 465.2498 1392.7276 1392.7249 1.97 1 66 1.3e-005 1 U R.TNAENEFVTIKK.D 550 738.3746 1474.7347 1474.7416 -4.68 0 82 3.5e-007 1 U K.WELLQQVDTSTR.T 551 738.3875 1474.7604 1474.7780 -11.91 0 97 1.3e-008 1 R.FLEQQNQVLQTK.W 603 546.9582 1637.8526 1637.8525 0.06 1 52 0.00035 1 U K.SLNNQFASFIDKVR.F

4. K1C10_HUMAN Mass: 58792 Score: 280 Matches: 7(4) Sequences: 7(4) emPAI: 0.28 Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 254 516.3011 1030.5876 1030.5910 -3.35 0 67 1.5e-005 1 U R.VLDELTLTK.A 329 555.2464 1108.4783 1108.4825 -3.78 0 18 0.89 1 K.DAEAWFNEK.S 414 412.2309 1233.6708 1233.6717 -0.74 1 51 0.00045 1 U R.LKYENEVALR.Q 502 691.3266 1380.6386 1380.6408 -1.62 0 95 1.9e-008 1 U R.ALEESNYELEGK.I 536 478.9227 1433.7464 1433.7626 -11.33 1 14 2.1 2 U K.IRLENEIQTYR.S 556 498.5796 1492.7169 1492.7270 -6.77 1 9 6.7 1 U R.SQYEQLAEQNRK.D 626 854.3834 1706.7522 1706.7649 -7.45 0 155 1.9e-014 1 U K.GSLGGGFSSGGFSGGSFSR.G

5. K22E_HUMAN Mass: 65393 Score: 277 Matches: 8(6) Sequences: 8(6) emPAI: 0.39 Keratin, type II cytoskeletal 2 epidermal OS=Homo sapiens GN=KRT2 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 197 487.2733 972.5321 972.5240 8.36 0 61 5.1e-005 1 K.IEISELNR.V 262 519.2675 1036.5204 1036.5189 1.45 0 40 0.0066 1 U R.YLDGLTAER.T 269 521.2765 1040.5384 1040.5502 -11.40 0 18 0.84 1 U K.VDPEIQNVK.A 328 554.2749 1106.5353 1106.5356 -0.28 0 47 0.0012 1 K.AQYEEIAQR.S 475 665.3600 1328.7055 1328.7187 -9.94 0 78 9.8e-007 1 R.NLDLDSIIAEVK.A 511 464.5644 1390.6715 1390.6728 -0.96 1 19 0.7 1 U R.SKEEAEALYHSK.Y 546 730.9027 1459.7908 1459.7922 -0.99 0 106 1.6e-009 1 U K.VDLLNQEIEFLK.V 551 738.3875 1474.7604 1474.7780 -11.91 0 97 1.3e-008 1 R.FLEQQNQVLQTK.W

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6. K1C9_HUMAN Mass: 62027 Score: 215 Matches: 5(3) Sequences: 5(3) emPAI: 0.19 Keratin, type I cytoskeletal 9 OS=Homo sapiens GN=KRT9 PE=1 SV=3 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 288 530.7865 1059.5585 1059.5560 2.29 0 57 0.00016 1 U K.TLLDIDNTR.M 411 616.7939 1231.5732 1231.5906 -14.06 0 125 2.2e-011 1 U R.SGGGGGGGLGSGGSIR.S 656 896.3657 1790.7169 1790.7205 -2.00 0 87 1.1e-007 1 U R.GGSGGSYGGGGSGGGYGGGSGSR.G 665 623.3169 1866.9288 1866.9145 7.67 1 21 0.33 1 U K.TLNDMRQEYEQLIAK.N + Oxidation (M) 729 724.3570 2170.0493 2170.0173 14.7 0 2 23 7 U K.SDLEMQYETLQEELMALK.K

7. CERU_HUMAN Mass: 122128 Score: 146 Matches: 3(3) Sequences: 3(3) emPAI: 0.09 Ceruloplasmin OS=Homo sapiens GN=CP PE=1 SV=1 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 499 686.3830 1370.7514 1370.7558 -3.21 0 51 0.00044 1 U K.GAYPLSIEPIGVR.F 535 716.3208 1430.6271 1430.6314 -2.96 0 93 2.8e-008 1 U R.QSEDSTFYLGER.T 669 638.0587 1911.1543 1911.1234 16.2 0 61 3.4e-005 1 U R.TTIEKPVWLGFLGPIIK.A

8. ALBU_HUMAN Mass: 69321 Score: 90 Matches: 2(2) Sequences: 2(2) emPAI: 0.11 Serum albumin OS=Homo sapiens GN=ALB PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 155 464.2484 926.4823 926.4861 -4.12 0 31 0.042 1 U K.YLYEIAR.R 604 547.3233 1638.9480 1638.9305 10.7 1 88 7.4e-008 1 U K.KVPQVSTPTLVEVSR.N

9. VIME_HUMAN Mass: 53619 Score: 68 Matches: 3(2) Sequences: 3(2) emPAI: 0.14 Vimentin OS=Homo sapiens GN=VIM PE=1 SV=4 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 341 561.3018 1120.5891 1120.5764 11.3 0 53 0.00035 1 R.EYQDLLNVK.M 577 785.9526 1569.8906 1569.8878 1.74 0 19 0.6 1 U R.ISLPLPNFSSLNLR.E 646 589.6108 1765.8104 1765.7974 7.37 1 45 0.0016 1 U R.LQDEIQNMKEEMAR.H + 2 Oxidation (M)

10. MED12_HUMAN Mass: 242928 Score: 39 Matches: 1(1) Sequences: 1(1) emPAI: 0.02 Mediator of RNA polymerase II transcription subunit 12 OS=Homo sapiens GN=MED12 PE=1 SV=4 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide

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583 796.8803 1591.7460 1591.7341 7.48 1 39 0.0061 1 U K.NKFGELFSDFCSK.V + Propionamide (C)

11. H6ST2_HUMAN Mass: 69086 Score: 35 Matches: 2(1) Sequences: 2(1) emPAI: 0.05 Heparan-sulfate 6-O-sulfotransferase 2 OS=Homo sapiens GN=HS6ST2 PE=2 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 501 460.5501 1378.6283 1378.6477 -14.04 1 35 0.02 1 U R.SEDESSARFVPR.Y 634 865.9033 1729.7921 1729.9264 -77.64 1 4 20 7 U R.NFHYITILRDPVSR.Y

12. CAPSD_HASV1 Mass: 85486 Score: 33 Matches: 1(1) Sequences: 1(1) emPAI: 0.04 Capsid polyprotein OS=Human astrovirus-1 GN=ORF2 PE=3 SV=1 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 653 594.3040 1779.8901 1779.9223 -18.08 0 33 0.023 1 U R.VTLLSTLVNQGMTMTR.A + Oxidation (M)

13. BIRC6_HUMAN Mass: 529919 Score: 31 Matches: 4(1) Sequences: 4(1) emPAI: 0.01 Baculoviral IAP repeat-containing protein 6 OS=Homo sapiens GN=BIRC6 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 114 445.3233 888.6320 888.4851 165 0 2 49 6 U K.LVNTLCR.K + Propionamide (C) 270 521.3089 1040.6032 1040.6593 -53.99 1 31 0.046 1 U K.LLVEQAKLK.Q 398 605.3140 1208.6135 1208.5534 49.7 0 7 11 3 U K.NTSHETAANHK.V 804 1152.8828 3455.6266 3455.8093 -52.87 1 6 15 9 U R.CISVVQKLVLFLLSMDFTCHADLLLFVCK.V + Oxidation (M); 2 Propionamide (C)

14. PNMA2_HUMAN Mass: 41483 Score: 28 Matches: 1(0) Sequences: 1(0) emPAI: 0.09 Paraneoplastic antigen Ma2 OS=Homo sapiens GN=PNMA2 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 62 423.7446 845.4747 845.5487 -87.50 1 28 0.12 1 U R.LLGKIFR.K

15. WDFY3_HUMAN Mass: 395006 Score: 28 Matches: 3(0) Sequences: 3(0) emPAI: 0.01 WD repeat and FYVE domain-containing protein 3 OS=Homo sapiens GN=WDFY3 PE=1 SV=2 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 34 410.7206 819.4266 819.4966 -85.43 1 28 0.14 1 U K.LLKQYR.V 519 703.8724 1405.7302 1405.8367 -75.71 1 4 26 7 U R.IKGYQLLAMLLK.K + Oxidation (M)

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806 888.3793 3549.4880 3548.6335 241 1 2 33 9 U R.LGTYESDKAMTVYECLSEWGQILCAICPNPK.L + Oxidation (M); Propionamide (C)

16. PTOV1_HUMAN Mass: 46839 Score: 19 Matches: 1(0) Sequences: 1(0) emPAI: 0.08 Prostate tumor-overexpressed gene 1 protein OS=Homo sapiens GN=PTOV1 PE=1 SV=1 Check to include this hit in error tolerant search or archive report

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 747 769.3849 2305.1330 2305.0442 38.5 1 19 0.47 1 U K.SLCRIMDNGFAGCVHFSYK.A + Oxidation (M); 2 Propionamide (C)

Peptide matches not assigned to protein hits: (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm Miss Score Expect Rank Unique Peptide 127 451.2382 900.4618 900.4817 -22.14 0 29 0.11 1 AEGGLWLR 573 783.8720 1565.7294 1565.7685 -25.02 1 28 0.081 1 NFSQVLENTSKGDK 111 443.2395 884.4645 884.5443 -90.30 1 26 0.15 1 LQKIDLR 445 635.8072 1269.5999 1269.6677 -53.39 1 26 0.16 1 NDVGGSPRVLEK 417 621.3227 1240.6308 1240.6888 -46.72 1 24 0.2 1 GKNLIPAAAGSSR 791 997.8486 2990.5241 2989.6391 296 0 24 0.16 1 MLDFAIFAVTFLLALVGAVLYLYPASR + Oxidation (M) 278 523.2807 1044.5468 1044.4836 60.5 0 23 0.31 1 ASEDGPLNSR 388 599.3377 1196.6609 1196.5543 89.1 1 23 0.31 1 CNLCGRSFR + 2 Propionamide (C) 369 584.2934 1166.5722 1166.6005 -24.30 0 22 0.38 1 FQDVLMSLAK + Oxidation (M) 495 684.3496 1366.6847 1366.7139 -21.41 1 22 0.35 1 VPGARAAACEPVR + Propionamide (C) 693 669.6521 2005.9343 2005.8687 32.7 1 22 0.3 1 MIGQFSYSEERESGENK + Oxidation (M) 508 692.8447 1383.6749 1383.6491 18.7 1 22 0.41 1 SSPESDRQVHSR 746 767.0617 2298.1633 2298.1289 15.0 1 22 0.28 1 FLIQCDWTMAGQQQGVFKK + Propionamide (C) 711 692.0133 2073.0180 2073.0524 -16.60 1 22 0.31 1 CVKEGVTNPSNSSQQALLK + Propionamide (C) 474 662.8294 1323.6443 1323.5118 100 1 22 0.41 1 CDPDTGQCRCR + Propionamide (C) 559 753.3728 1504.7311 1504.8323 -67.24 1 21 0.4 1 KFPLAQTESLLMK 492 677.3415 1352.6685 1352.7048 -26.80 1 21 0.44 1 LELPRASDAEPR 289 531.7896 1061.5646 1061.5605 3.89 0 21 0.6 1 TVTVTLGDEK 300 535.3100 1068.6054 1068.6080 -2.44 0 20 0.56 1 LFPVLPGNGR 129 453.2923 904.5701 904.4515 131 1 20 0.71 1 ARGNWSSK 377 589.2961 1176.5776 1176.6503 -61.74 0 20 0.74 1 LTAFVNTLNGK 301 536.2888 1070.5631 1070.6237 -56.55 0 19 0.81 1 LLALAGGPGFR 236 507.8130 1013.6114 1013.6121 -0.63 1 19 0.83 1 VDAKVEIIK 685 657.0381 1968.0923 1967.9663 64.0 0 19 0.54 1 VLVDMDGVLADFEGGFLR + Oxidation (M) 536 478.9227 1433.7464 1433.9082 -112.83 1 19 0.73 1 LARILLQEIPIR 64 425.2143 848.4140 848.3810 38.8 0 19 0.94 1 NNGSCINK 614 553.9765 1658.9077 1658.7942 68.4 0 19 0.7 1 MCSTPGMPAPGASLALR 444 635.3183 1268.6221 1268.6837 -48.55 1 18 0.83 1 QNPTERLGNLK 255 516.7741 1031.5336 1031.6491 -111.98 1 18 1.1 1 LVLLFSGKR 448 427.2000 1278.5782 1278.6415 -49.56 1 18 0.85 1 EASSSGITNGKTK 472 657.8749 1313.7353 1313.6980 28.4 0 18 0.85 1 NFSGSIPAVTPPK 607 822.4500 1642.8855 1642.8211 39.2 1 18 0.76 1 LIYCLMAKDSFPR + Oxidation (M); Propionamide (C)

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686 657.0599 1968.1578 1967.9921 84.2 1 18 0.65 1 GGIMSLTEVYCLVNRAR + Oxidation (M); Propionamide (C) 554 740.3940 1478.7734 1478.7803 -4.64 0 18 0.84 1 VVTYGMANLLTGPK + Oxidation (M) 494 455.2000 1362.5782 1362.7031 -91.67 0 18 1 1 VPAGSDVISEYVK 227 505.2686 1008.5227 1008.4885 33.9 0 18 0.96 1 MVSWMISR 447 637.2680 1272.5214 1272.5842 -49.37 1 18 0.97 1 SKYMTPMQQK + 2 Oxidation (M) 582 530.9478 1589.8216 1589.7654 35.4 0 18 0.89 1 QCLENQQLIMQR + Oxidation (M); Propionamide (C) 250 514.7438 1027.4730 1027.5927 -116.48 1 17 1.1 1 IIYQAHKR 733 737.7038 2210.0897 2210.0644 11.4 1 17 0.76 1 TVNLTDFSNKWDSTNPFPK 109 442.7596 883.5047 883.5127 -9.05 0 17 1.1 1 LSLPADIR 364 583.2922 1164.5698 1164.6026 -28.18 0 17 1.1 1 GSFEGLESLVK 381 592.8657 1183.7169 1183.6561 51.4 0 17 1.2 1 DLLQLGGELAR 389 400.2376 1197.6910 1197.6618 24.4 1 17 1.2 1 ILKNHPSYAR 13 400.2130 798.4115 798.4599 -60.71 0 17 1.3 1 AVQGLSPK 393 603.7951 1205.5756 1205.5533 18.5 0 17 1.3 1 CATCSQPILDR 361 582.3147 1162.6148 1162.5699 38.6 1 16 1.4 1 LVGGTNRCMGR 640 585.3275 1752.9605 1752.9266 19.4 1 16 1 1 YDMSGARLALILCVTK 260 518.2763 1034.5380 1034.4525 82.7 0 16 1.4 1 MVDMPANNK + Oxidation (M) 609 550.9324 1649.7754 1649.8889 -68.82 1 16 1.2 1 IKDNAADWHNLILK 539 482.5776 1444.7111 1444.6794 21.9 0 16 1.4 1 SVETDSSTVEHVR 624 848.5008 1694.9870 1694.8225 97.0 1 16 1.3 1 LYEFYLSKCSESVK 431 629.2879 1256.5612 1256.6071 -36.53 0 16 1.5 1 MPTPDATTPQAK 253 515.2912 1028.5679 1028.5614 6.31 1 16 1.6 1 EQKILNER 647 590.3000 1767.8782 1767.9077 -16.70 0 16 1.3 1 GTTEIGMIGSKPFSTVK + Oxidation (M) 193 485.2519 968.4892 968.4862 3.09 1 16 1.5 1 YRMQSIR + Oxidation (M) 704 1033.5422 2065.0698 2065.0229 22.8 0 16 1.1 1 ATSEIFHSQSFLATGSNLR 325 551.8699 1101.7252 1101.5964 117 1 16 1.8 1 RTMIISPER 327 553.3065 1104.5984 1104.5663 29.1 0 16 1.8 1 QETDSSLVVK 716 705.7083 2114.1031 2114.0864 7.94 1 16 1.2 1 LQHMTLAEDVKAMLTELR + Oxidation (M) 419 415.2000 1242.5782 1242.5737 3.64 1 16 1.6 1 CSECGKAFSLK + Propionamide (C) 423 624.8365 1247.6585 1247.6245 27.3 1 15 1.9 1 KDEELSSALEK 510 694.3745 1386.7345 1386.7044 21.7 0 15 1.6 1 HYALKPDAYPGR 783 695.3471 2777.3594 2777.4496 -32.47 0 15 1.1 1 MAGSPTCLTLIYILWQLTGSAASGPVK 201 489.2718 976.5291 976.4574 73.4 0 15 2.2 1 ASNETQTAR 241 510.2890 1018.5634 1018.5368 26.1 1 15 2.2 1 KILEEMEK 688 660.3839 1978.1299 1977.9690 81.3 1 15 1.4 1 HIKTLSEAHEDCVNNIR 558 752.8692 1503.7238 1503.7569 -22.02 0 15 1.6 1 QAIELSPDNQYVK 243 511.2490 1020.4834 1020.5022 -18.46 0 15 2.1 1 GSLTGVQTCR 128 452.2556 902.4967 902.4934 3.67 1 15 2.4 1 ARGISGSAGK 771 644.8154 2575.2326 2575.5101 -107.78 1 15 1.2 1 FRVGFLIISSSSSLSPLLLVSVVR 777 889.1351 2664.3835 2664.3038 29.9 1 15 1.2 1 ITDQEASENHVAATGSHLCVLRSR + Propionamide (C) 710 1037.5029 2072.9912 2073.0313 -19.37 1 15 1.5 1 MHLQFDRQTGDLLLNEK + Oxidation (M) 284 527.3028 1052.5910 1052.6593 -64.97 0 15 2 1 LIVNLKPEK 778 1337.1038 2672.1931 2672.4306 -88.87 0 15 1.2 1 IVSDQNMQILLLSSLTGDLLLNEK + Oxidation (M) 570 778.4199 1554.8252 1554.7139 71.6 1 15 1.7 1 GCCMEIMSLRAAVR + Oxidation (M) 691 662.3741 1984.1003 1984.0338 33.6 1 15 1.5 1 SDLLKAGGGAGGSGGSGPSAILR

Downloaded from iovs.arvojournals.org on 09/26/2021 10 of 23 8/06/2012 1:09 PM Peptide Summary Report (Submitted from PROJ13880 LingZhu by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120608/F077324.dat&REPTYPE=pe...

89 434.7618 867.5091 867.4450 73.9 1 15 2 1 RDISSYK 532 475.9688 1424.8845 1424.8174 47.1 1 14 2 1 DIRVNCVVPGIIK 606 821.9075 1641.8005 1641.8046 -2.48 1 14 1.8 1 LSAWVREQCPGPGSR 772 861.0967 2580.2684 2580.1243 55.9 1 14 1.2 1 HGRPCSACPPSFGGGCRENLCYK + 2 Propionamide (C) 302 536.3217 1070.6288 1070.5972 29.6 0 14 2.5 1 LQGTDIPTVK 135 456.7957 911.5768 911.4278 163 0 14 2 1 LLASMMCK + Oxidation (M) 561 504.2293 1509.6660 1509.7246 -38.82 1 14 2 1 ARSGTFDLLEMDR 755 803.7316 2408.1730 2408.2497 -31.83 1 14 1.4 1 RQVLFLCVFLGMSWAGAEPLR + Oxidation (M) 659 897.9185 1793.8225 1793.8028 11.0 0 14 1.9 1 TGTTGQSGAESGTTEPSAR 21 402.7227 803.4309 803.4653 -42.89 1 14 3.3 1 IPKQYR 575 784.3726 1566.7307 1566.7058 15.9 1 14 2.2 1 WMIPSEAKESMDK + Oxidation (M) 776 666.0899 2660.3304 2660.2096 45.4 1 14 1.4 1 QAEQLSAAGEGGDAGRMDTEEARPAK + Oxidation (M) 345 564.8555 1127.6964 1127.6047 81.3 1 14 2.5 1 RTQQIPETR 59 421.7567 841.4988 841.4658 39.3 0 14 2.4 1 TAVAPLDR 425 417.2130 1248.6172 1248.6251 -6.36 0 14 2.5 1 FTFRPPNNEK 592 808.4186 1614.8226 1614.8188 2.36 1 14 2.1 1 WMDVVVDDRLPVR + Oxidation (M) 399 404.5493 1210.6259 1210.7285 -84.73 0 14 2.3 1 GIKPVTLELGGK 199 488.2700 974.5254 974.3474 183 0 14 3.1 1 SCDFGDGMK + Oxidation (M) 785 956.4814 2866.4225 2866.4034 6.67 0 14 1.5 1 ADMWSCGVILFALLVGALPFDDDNLR + Oxidation (M) 391 402.5386 1204.5939 1204.4852 90.2 0 14 2.7 1 SMMSSYSAADR 515 700.7811 1399.5476 1399.6120 -46.04 1 14 2.4 1 CCVPVCYQCKI + 2 Propionamide (C) 581 794.4199 1586.8252 1586.8450 -12.45 0 14 2.3 1 ISMQNLEALLVANR + Oxidation (M) 291 532.3897 1062.7648 1062.5054 244 1 14 2.8 1 SSGSEGDRLR 465 653.3621 1304.7097 1304.6105 76.1 0 14 2.6 1 HLTIMMDIDGK + 2 Oxidation (M) 372 585.8632 1169.7118 1169.6292 70.6 1 13 2.6 1 KDGSSPPLLEK 703 688.0347 2061.0821 2061.1333 -24.81 0 13 2 1 FVALICEASGGGAFLVLPLGK 398 605.3140 1208.6135 1208.5868 22.1 1 13 2.7 1 CLLWACKACK + Propionamide (C) 237 508.7835 1015.5524 1015.4869 64.5 1 13 3.4 1 LCRGPEDR + Propionamide (C) 516 700.8913 1399.7680 1399.5999 120 0 13 2.7 1 EPSVDDYMGMIK + Oxidation (M) 683 983.9801 1965.9456 1965.8958 25.3 1 13 2.1 1 MEDSPTMVRVDSPTMVR + Oxidation (M) 567 512.8995 1535.6768 1535.7137 -24.06 1 13 2.5 1 EEVDNTEGLKGSMK 451 643.2981 1284.5816 1284.5874 -4.54 0 13 2.8 1 DFSESEFPSIK 645 588.9815 1763.9227 1763.9492 -15.01 0 13 2.7 1 TLVVTTILENPYVMR + Oxidation (M) 565 764.9147 1527.8149 1527.8595 -29.24 1 13 2.6 1 LQPMRATVPFQLK 228 505.2707 1008.5269 1008.4447 81.5 1 13 3 1 RGEECPYR 422 416.5563 1246.6469 1246.6777 -24.70 1 13 3.1 1 KILTLPEMMR + Oxidation (M) 651 592.9464 1775.8172 1775.9543 -77.18 1 13 2.6 1 HRQPPAATAPAPAAPAPR 337 559.2887 1116.5628 1116.6139 -45.78 1 13 3.6 1 AVKDATNTGIK 122 449.7282 897.4419 897.5760 -149.39 1 13 2.9 1 VLALQARK 387 597.3337 1192.6528 1192.5845 57.3 1 13 3.1 1 VHLYKCGAMR + Oxidation (M) 649 590.6495 1768.9268 1768.9182 4.84 0 13 2.5 1 MNYVGQLAGQVFVTVK + Oxidation (M) 743 763.0869 2286.2388 2286.2042 15.2 1 13 2 1 ATDYPLIARILQGPCEQISK + Propionamide (C) 138 457.2346 912.4546 912.4231 34.5 0 13 3.2 1 VMTMMIR + 2 Oxidation (M) 332 556.3170 1110.6194 1110.5782 37.1 0 13 2.9 1 LGTSAEGAHLR 684 656.3253 1965.9541 1965.9367 8.86 0 13 2.4 1 LTADSSHYLCHEVAPPR + Propionamide (C)

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617 556.9665 1667.8777 1667.6911 112 0 13 2.7 1 GAAAPEEEDEEPGDPR 507 692.8398 1383.6650 1383.5482 84.4 1 13 3.2 1 HTCNECGKSFCR 93 435.7645 869.5144 869.4607 61.8 0 13 3.1 1 QEIDIPR 349 570.2939 1138.5733 1138.5441 25.7 1 13 3.2 1 TDRVMFQDK 562 756.4114 1510.8082 1510.7524 37.0 1 13 3 1 GVFAMKGCEELLK + Oxidation (M); Propionamide (C) 792 1005.1837 3012.5293 3012.3935 45.1 0 13 2.5 1 DEESSLETFVSALESLLTSPESTQEER 223 503.3672 1004.7198 1004.4709 248 0 12 3.8 1 MGQSLQEGR 658 598.6523 1792.9352 1792.6420 164 1 12 2.8 1 GCCGSCGGSKGGCGSCGGSK + 2 Propionamide (C) 320 550.2509 1098.4872 1098.5896 -93.16 0 12 3.7 1 IFCFILSTR 760 634.3053 2533.1920 2533.2152 -9.14 0 12 2.1 1 EDIVNQMTEACLNQSLDALLSR + Propionamide (C) 513 697.8715 1393.7285 1393.7565 -20.12 1 12 3.2 1 IEVENKEVLHGK 478 667.3186 1332.6226 1332.6397 -12.86 1 12 3.5 1 YGSAVFRGCFR + Propionamide (C) 352 572.2634 1142.5123 1142.6309 -103.77 1 12 4 1 AQAVWSRLGR 172 473.2256 944.4367 944.4062 32.3 0 12 4.7 1 YCPSYNK + Propionamide (C) 455 648.2866 1294.5586 1294.7179 -123.04 1 12 3.2 1 TLEHLCKKPR + Propionamide (C) 594 540.5849 1618.7328 1618.7937 -37.66 1 12 3 1 EEIVTPKETETSEK 408 409.8851 1226.6334 1226.6805 -38.38 1 12 3.5 1 RVVPPASNMLK + Oxidation (M) 805 1158.5561 3472.6464 3472.4080 68.7 0 12 3.2 1 SSAQCLPPASSCCAPRPGCCGGPSSEGGCCLSHHR + Propionamide (C) 346 568.2895 1134.5645 1134.5227 36.8 0 12 4 1 DEGPMVSASVK + Oxidation (M) 168 471.7800 941.5454 941.5069 40.8 0 12 3.7 1 IIDPEDLK 435 421.8888 1262.6446 1262.5900 43.3 0 12 3.7 1 AFCLHQEIMR + Oxidation (M) 74 428.7604 855.5062 855.5178 -13.53 0 12 3.8 1 LQVIDIR 376 589.2823 1176.5501 1176.5267 19.9 0 12 4.1 1 IEVMENPSCR 410 615.2911 1228.5677 1228.5910 -19.00 0 12 3.7 1 ISEQFTAMFR 660 599.9817 1796.9234 1796.8008 68.2 0 12 3 1 EQTMWACLAAMAVANR + 2 Oxidation (M) 586 800.4843 1598.9540 1598.7763 111 1 12 3.4 1 FFGDSAASMAIKNPK + Oxidation (M) 330 555.2598 1108.5051 1108.5764 -64.37 0 12 3.7 1 ITDLYTDLR 343 564.2800 1126.5455 1126.6499 -92.65 0 12 3.8 1 IIHTGAKPYK 644 588.3067 1761.8982 1761.9890 -51.50 1 12 3.3 1 IGLIQHLPKGVYDPGR 344 564.3205 1126.6264 1126.6896 -56.12 0 12 3.8 1 LIVALMKPSR 608 413.1000 1648.3709 1647.8832 296 1 12 3.3 1 VYTITPLLSDNREK 613 829.4320 1656.8495 1656.8332 9.83 1 12 3.2 1 APEGGEGAQPVPGHARK 700 682.0030 2042.9871 2042.7979 92.6 0 12 2.9 1 EQQLESCACNETDNSCK + 2 Propionamide (C) 299 535.2837 1068.5529 1068.4481 98.1 0 12 3.8 1 SMFHTQMR + 2 Oxidation (M) 424 417.2000 1248.5782 1248.6132 -28.06 1 12 4.1 1 CEALAVGRSSEK 133 455.2764 908.5383 908.5080 33.4 0 12 4.4 1 ATATPVPPR 73 428.7192 855.4239 855.4562 -37.75 1 12 4.5 1 SHLSEKR 786 960.4852 2878.4338 2878.2683 57.5 1 12 2.9 1 MPKSEGGSGGGAAGGGAGGAGAGAGCGSGGSSVGVR + Oxidation (M); Propionamide (C) 795 1021.2068 3060.5986 3060.4063 62.8 1 11 3.4 1 GASCQGCPNQRLCASGAGATPDTAIEEIK + 3 Propionamide (C) 219 499.2367 996.4589 996.4984 -39.58 1 11 4.2 1 MISEKMVK + 2 Oxidation (M) 725 1077.5042 2152.9939 2153.0748 -37.59 1 11 3 1 VEVTECSDGLFCKTLTIPK + Propionamide (C) 531 713.3893 1424.7640 1424.8463 -57.81 1 11 4 1 TTLILPTQQVRR 682 653.0454 1956.1143 1956.0568 29.4 0 11 3.4 1 LVYSQPLDLPESVEVIR 147 461.7426 921.4706 921.4590 12.6 0 11 4.4 1 MVTAQTQK + Oxidation (M) 800 1027.8565 3080.5478 3080.5100 12.3 1 11 3.2 1 SITPLGGFPHYGEVNNDFVMLKGCIAGTK + Oxidation (M)

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395 604.3298 1206.6451 1206.5881 47.3 0 11 4.6 1 VVAEVYDQER 204 491.2681 980.5216 980.4927 29.5 0 11 4.6 1 AFNNSSTLK 311 542.8065 1083.5985 1083.5924 5.64 1 11 4.6 1 STHEKILEK 768 855.7998 2564.3775 2564.2165 62.8 1 11 3.1 1 QVDHHCKDTTLIHQIFGGCWR + Propionamide (C) 134 456.3207 910.6268 910.4542 190 1 11 4.2 1 SSSMKSLR + Oxidation (M) 615 555.6009 1663.7810 1663.8240 -25.83 0 11 4.2 1 ACYLVLGTEEPGTGVR 799 1026.5315 3076.5728 3076.5038 22.4 1 11 3.5 1 LKYQVLDGEMYPPSVEEAPVLMHYPR + Oxidation (M) 572 781.9052 1561.7959 1561.8690 -46.84 0 11 4.3 1 AYILLGQFLLMHK + Oxidation (M) 790 985.1942 2952.5607 2952.4129 50.1 1 11 3.6 1 EISKVMASMNLGLLSEGGCSEDELLEK + Propionamide (C) 217 498.2691 994.5236 994.4138 110 0 11 4.7 1 ACEGTGSGSR + Propionamide (C) 430 627.8719 1253.7292 1253.6842 35.9 1 11 4.7 1 KLVDQFFVMK 695 671.6635 2011.9686 2011.8898 39.1 0 11 3.5 1 FQFELTLDEEDSEGPEK 690 662.3716 1984.0930 1984.0338 29.9 1 11 3.5 1 SDLLKAGGGAGGSGGSGPSAILR 370 585.2771 1168.5397 1168.7179 -152.55 1 11 5 1 SVTLLIKGPNK 322 551.3077 1100.6009 1100.5437 52.0 0 11 5.2 1 FVIHHSMSK + Oxidation (M) 696 507.2993 2025.1683 2024.9148 125 1 11 3.4 1 HSSTGDSADAGPPAAGSARGEK 798 1026.4952 3076.4636 3075.6209 274 1 11 3.5 1 MAAAPRTVLISGCSSGIGLELAVQLAHDPK + Propionamide (C) 446 424.8164 1271.4273 1271.5630 -106.66 0 11 4.8 1 SSFSSTSESPTR 674 970.9660 1939.9175 1940.0765 -81.94 0 11 3.7 1 LLPLLLSSLLGGSQAMDGR 374 587.2883 1172.5621 1172.4777 72.0 0 11 5.7 1 CAMCQSIFDR 354 573.3004 1144.5863 1144.6241 -33.04 1 11 5.4 1 HVVSVFKDSK 642 878.9162 1755.8178 1755.8315 -7.80 0 11 4.3 1 AYEEYVLSVGNLDER 224 505.2112 1008.4078 1008.5678 -158.59 1 11 5 1 LKMTYLPK + Oxidation (M) 797 769.1330 3072.5030 3073.2391 -239.52 0 11 3.8 1 MHPNVSQGCQGGCATCSDYNGCLSCKPR + Oxidation (M); 2 Propionamide (C) 161 469.2231 936.4317 936.4889 -61.17 0 11 5.2 1 AHNGALQAR 781 921.1740 2760.5001 2760.2919 75.4 1 11 4.2 1 AQEILDESHVNSRMGCQATQLTSR + Oxidation (M); Propionamide (C) 115 446.2321 890.4497 890.3916 65.2 0 11 6.6 1 AVDEMNGR 584 799.9116 1597.8086 1597.8311 -14.09 0 11 4.5 1 ISDLGLATEIPEGQR 44 416.2261 830.4377 830.4722 -41.55 1 11 6.9 1 VVSGASRR 809 1022.4860 4085.9149 4086.0017 -21.23 0 10 4.4 1 CPSPGCQEAFLTFLCVMCICSLIGAMAQTPSVIILIR + Oxidation (M); 2 Propionamide (C) 251 515.2612 1028.5078 1028.5291 -20.71 0 10 5.8 1 AFSYSSVLR 664 911.4431 1820.8716 1820.8767 -2.78 0 10 4.8 1 SYLCHYELAEALPQGK 436 632.8384 1263.6623 1263.6207 32.9 1 10 5.5 1 SRNIGSNYPEK 678 973.4468 1944.8790 1945.0972 -112.15 1 10 4.1 1 FMVTQILVALRNLHFK + Oxidation (M) 326 553.2687 1104.5228 1104.5485 -23.29 1 10 6.3 1 CDDLEALKK + Propionamide (C) 714 1041.5382 2081.0619 2081.1781 -55.81 0 10 3.8 1 VFLLCAYILLLMVSQLR + Oxidation (M); Propionamide (C) 418 415.1000 1242.2782 1242.5598 -226.61 1 10 3.2 1 GGTISCYRCGR + Propionamide (C) 517 467.9329 1400.7770 1400.7565 14.7 1 10 5.1 1 VRLQHFFTPEK 421 623.3334 1244.6523 1244.6473 4.00 1 10 5.7 1 TGPLSTSSPSRR 522 710.4158 1418.8170 1418.6830 94.5 1 10 5.3 1 EFFESNIKSYR 469 655.3223 1308.6300 1308.6098 15.4 0 10 5.3 1 GNFIPYANEER 471 656.3132 1310.6118 1310.6102 1.22 0 10 5.3 1 QLSYSDNQASAK 175 474.3438 946.6730 946.5348 146 0 10 6.3 1 HPSPAIVAR 774 649.1000 2592.3709 2592.2849 33.2 1 10 3.7 1 TVSNLILTGPRTIVMEVMEELEC + Oxidation (M) 171 472.3740 942.7335 942.5386 207 0 10 4.7 1 EINDLLVK

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460 649.2746 1296.5346 1296.6422 -83.00 1 10 5.3 1 KNHEEEVGSLR 413 617.7952 1233.5759 1233.7346 -128.67 1 10 6 1 VHVILHFKNK 106 441.2580 880.5014 880.5317 -34.39 0 10 6 1 IVLLMHR 727 541.2273 2160.8802 2161.0144 -62.06 1 10 4 1 SLDKDPMLLSGTHVMEGSGR + 2 Oxidation (M) 185 479.2458 956.4770 956.6423 -172.77 1 10 6.6 1 LLPLKFVK 543 486.2000 1455.5782 1455.8045 -155.48 1 10 5.3 1 LNTVRQIIEQDK 638 873.9002 1745.7858 1745.7973 -6.57 0 10 4.9 1 QLLAMLMLFCDCSR + 2 Oxidation (M); Propionamide (C) 794 1019.8287 3056.4642 3056.4841 -6.51 0 10 4.6 1 ALQCAAGGNVNVEMNAAPGVDLAVLLNNMR + 2 Oxidation (M) 30 407.1737 812.3328 812.4691 -167.73 1 10 5.9 1 IMKHLR + Oxidation (M) 622 841.9026 1681.7907 1681.8756 -50.44 1 10 4.8 1 SIHCAPMLSDILRR + Propionamide (C) 47 417.2000 832.3854 832.4403 -65.85 0 10 7.8 1 KPTASSSR 611 415.1000 1656.3709 1656.7310 -217.35 0 10 5.1 1 MPSCDPGPGPACLPTK + Oxidation (M); Propionamide (C) 485 673.3587 1344.7029 1344.7666 -47.36 1 10 6.3 1 YHAALAVLKGFR 576 523.2720 1566.7942 1566.8042 -6.41 1 10 5.8 1 YPHTSATFLTSSKK 426 627.2817 1252.5489 1252.6169 -54.23 1 10 6.4 1 CLFCRIGGSR + 2 Propionamide (C) 357 575.3250 1148.6354 1148.6046 26.9 0 10 6.9 1 LMSIMGNVLR + Oxidation (M) 545 487.2316 1458.6729 1458.6595 9.14 1 10 6.1 1 DDAPTCGICHKTK + Propionamide (C) 718 1061.5060 2120.9974 2121.0176 -9.53 0 10 4.8 1 QWLCDFGFLVDIMEHLR 149 462.2489 922.4832 922.5600 -83.18 1 10 6.9 1 KLLHGDIK 740 758.7393 2273.1960 2273.0272 74.3 0 10 4.5 1 AEGYPVDLYYLMDLSYSMK + Oxidation (M) 43 415.2077 828.4008 828.5433 -171.91 1 10 8 1 ILNKLTK 336 557.7974 1113.5802 1113.6254 -40.66 1 9 7.3 1 VAAQRDSVLR 500 458.6031 1372.7874 1372.7212 48.3 1 9 6.7 1 DPRGFQGGVLTAR 139 458.2284 914.4422 914.5437 -111.00 0 9 8.2 1 ADSLLAVVK 796 1025.1559 3072.4458 3073.3402 -291.00 1 9 5.2 1 MGSEDHGAQNPSCKIMTFRPTMEEFK + 2 Oxidation (M); Propionamide (C) 529 475.2219 1422.6440 1422.6207 16.4 0 9 6.2 1 CGTMLCHDFLGR + Propionamide (C) 707 519.1381 2072.5235 2073.0854 -271.08 1 9 9.1 1 EPEPEPAAVEVPAGRVLSAR 503 461.5617 1381.6632 1381.6038 43.0 0 9 6.4 1 SSDVYVSYDYGK 780 689.3455 2753.3530 2753.3927 -14.45 1 9 4.2 1 VARQSLTMFVLIMNGCHIEIDAHR 468 436.8830 1307.6271 1307.5850 32.2 0 9 6.9 1 QCDLAGVETCK + 2 Propionamide (C) 432 629.3187 1256.6229 1256.6724 -39.42 0 9 7.2 1 VEAEALNATAIR 662 453.2218 1808.8582 1808.8167 23.0 1 9 6 1 FFFMNPCEKFWAR + Oxidation (M); Propionamide (C) 51 418.2494 834.4842 834.4460 45.7 1 9 8.4 1 GVSRGGFR 39 414.2174 826.4203 826.4232 -3.44 1 9 6.6 1 AHMNAKR 434 421.5469 1261.6188 1261.7064 -69.38 1 9 7.5 1 ESLTKLMATLR 701 685.3671 2053.0796 2053.0626 8.29 0 9 5.1 1 CSATPSAQVKPIVSASPPSR + Propionamide (C) 802 807.3538 3225.3860 3225.5483 -50.34 1 9 6.2 1 AFSTCSSHLCVVGLFFGSAIIMYMAPKSR + 2 Oxidation (M); Propionamide (C) 233 507.2802 1012.5458 1012.5706 -24.47 0 9 7.1 1 LPWAGQLTK 757 1217.2988 2432.5831 2432.1716 169 1 9 8.6 1 EDQCLMPEAWNVDQGVITKLK + Oxidation (M) 390 602.3268 1202.6390 1202.5026 113 0 9 8 1 FNFDEMQTR + Oxidation (M) 380 591.3050 1180.5954 1180.4498 123 0 9 7.3 1 CMGPMTHCTGK + Oxidation (M) 117 447.1599 892.3052 892.5382 -261.04 0 9 8.4 1 AVTPAPPIK 42 415.2000 828.3854 828.4858 -121.08 0 9 9 1 ALTHFLK 676 972.9982 1943.9818 1943.9014 41.4 0 9 5.5 1 EESSPVGFPGTPTWNPSR 316 544.2953 1086.5761 1086.6219 -42.19 0 9 9.1 1 ALILLGQMGR + Oxidation (M)

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650 886.9136 1771.8127 1771.9369 -70.13 1 9 5.7 1 LFAPWRGLQELTDAR 231 507.2333 1012.4520 1012.5607 -107.29 1 9 7.5 1 FFIAGFRR 385 596.2867 1190.5589 1190.6078 -41.01 1 9 8 1 DVVAMARTGSGK 296 533.3003 1064.5861 1064.5437 39.9 0 9 8.2 1 YCQVLNAVR 295 533.2850 1064.5555 1064.5648 -8.74 1 9 8.3 1 KLTGSTVGMR + Oxidation (M) 252 515.2805 1028.5464 1028.5251 20.8 0 9 8.5 1 ALEGGQAEVR 124 450.2339 898.4533 898.4621 -9.75 1 9 7.5 1 SSRGHDLK 694 1006.4829 2010.9512 2011.1102 -79.07 1 9 6.2 1 SDLLLKELYVENAHLVR 591 805.4383 1608.8621 1608.9199 -35.93 1 9 6.9 1 LSSLEGLHTKLGNIK 699 1021.5454 2041.0763 2041.1241 -23.43 0 9 5.3 1 SLAATPSSSLPCTLIALGLR + Propionamide (C) 602 546.9272 1637.7597 1637.7112 29.6 0 9 7 1 LCMHACVNTPGSYR + Oxidation (M); Propionamide (C) 86 433.2620 864.5094 864.4011 125 1 9 8.5 1 QSMLEKGG + Oxidation (M) 174 474.2758 946.5370 946.5083 30.3 0 9 9.5 1 AEALSSTLR 177 475.7294 949.4442 949.4844 -42.27 0 9 9.3 1 MFYHKPK 708 519.1403 2072.5321 2072.7784 -118.81 0 9 11 1 FHHCDMYGSACADCCLAR + Propionamide (C) 744 764.0672 2289.1798 2289.1199 26.2 1 9 5.7 1 EKFTTPIEETGGEGCPAVALIQ 167 471.2767 940.5388 940.5202 19.7 1 9 8.1 1 ERPVERR 189 482.3636 962.7126 962.4743 248 0 9 9.1 1 MGEIADAIK + Oxidation (M) 198 488.2616 974.5087 974.4967 12.3 0 9 10 1 AAAATMAAAAR 518 701.8885 1401.7624 1401.7113 36.4 1 8 8.6 1 QSLISAGDYRHR 144 459.2050 916.3954 916.4436 -52.63 0 8 11 1 CSAPPTVSR 564 760.8515 1519.6885 1519.6945 -3.99 0 8 7.9 1 CHLGISASEMVMR + Oxidation (M); Propionamide (C) 453 645.8392 1289.6639 1289.6108 41.2 0 8 8.5 1 ETAKPCLSNPCK 26 406.2236 810.4325 810.4096 28.3 0 8 7.8 1 NVTANHR 386 596.3168 1190.6190 1190.5601 49.4 0 8 9.2 1 VLSAMPDGTER + Oxidation (M) 479 668.3503 1334.6860 1334.7050 -14.23 1 8 8.6 1 QGSMIQTLAMKK 350 570.3030 1138.5914 1138.5229 60.1 0 8 8.5 1 YIEMQHFR + Oxidation (M) 593 539.2857 1614.8352 1614.7502 52.6 1 8 7.7 1 CQSILNMAMFRCK + Propionamide (C) 405 408.2147 1221.6223 1221.5738 39.7 1 8 9.4 1 NFAKGSEEQGR 598 813.4322 1624.8498 1624.7692 49.6 1 8 7.6 1 QEAELGEPDPEQKR 37 413.1000 824.1854 824.3664 -219.53 0 8 5.2 1 EFSSEAR 806 888.3793 3549.4880 3549.7378 -70.37 1 8 7.4 1 TESSSDRIPVHQNGLSMNQMLMGLSPNVLPGPK + Oxidation (M) 234 507.2887 1012.5628 1012.5777 -14.73 1 8 8.8 1 QLEAQIRR 91 435.2393 868.4641 868.3385 145 0 8 7.9 1 EGMDDFR 457 648.3131 1294.6116 1294.6454 -26.07 0 8 8.2 1 MIIWFPDMVK + Oxidation (M) 619 836.9142 1671.8138 1671.8362 -13.42 1 8 7.7 1 SAMDDTGTKRPPKPR + Oxidation (M) 186 479.3245 956.6344 956.3909 255 0 8 10 1 SESFDCAK + Propionamide (C) 648 884.9510 1767.8874 1767.8648 12.8 1 8 7.8 1 QVSQQPKGIMGMFASK + 2 Oxidation (M) 706 519.1360 2072.5148 2071.9952 251 1 8 12 1 SELLMIIQMETQSSMRK + 3 Oxidation (M) 40 414.7625 827.5105 827.3522 191 0 8 10 1 HGGGGGDSGK 335 557.2746 1112.5347 1112.6052 -63.37 1 8 9.2 1 FFQMVGLKK + Oxidation (M) 383 593.2850 1184.5555 1184.4591 81.4 0 8 11 1 MYGCDVGPDGR + Oxidation (M) 196 487.2295 972.4444 972.5352 -93.42 1 8 11 1 RDGVVSVNK 555 494.9360 1481.7862 1481.8426 -38.08 1 8 7.7 1 AQVRAAGTALGQALR 220 502.2831 1002.5517 1002.5835 -31.73 1 8 11 1 AHLIRHTR

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585 533.9625 1598.8657 1598.8026 39.5 1 8 8.4 1 HRHIGSVHVTEDGR 625 427.2000 1704.7709 1704.8431 -42.36 0 8 8.1 1 LVSHPEEPVAGAQTDR 600 542.9652 1625.8739 1625.8988 -15.35 0 8 8.3 1 SLVVETQDLASPVLR 766 640.8122 2559.2197 2559.2362 -6.45 1 8 5.8 1 DSSLPQAACHPAIFRVVDEMFR + Propionamide (C) 514 699.8848 1397.7550 1397.7476 5.33 0 8 8.8 1 LEPMIVPDLDLK + Oxidation (M) 547 732.3741 1462.7335 1462.8184 -58.01 0 8 9.2 1 IVLDTFVYQIPR 245 511.3538 1020.6930 1020.5240 166 0 8 11 1 AFETVAIDR 360 577.3259 1152.6372 1152.5155 106 0 8 9.7 1 GVNMEELMSK + Oxidation (M) 240 509.2488 1016.4830 1016.5688 -84.46 0 8 11 1 NSINLMVVK 741 761.3918 2281.1535 2281.0831 30.9 0 8 6.7 1 ALQNCCSVPSPLPPEDAGTGGLR 597 542.2622 1623.7649 1623.8766 -68.80 1 8 8.3 1 RPLETPPEVVCTKR 519 703.8724 1405.7302 1405.7677 -26.69 1 8 9.7 1 NIAIQSQFTRTK 612 415.2000 1656.7709 1656.8731 -61.70 1 8 8.2 1 MKSPPLLSPCLSWK + Propionamide (C) 305 537.2598 1072.5051 1072.6128 -100.45 1 8 12 1 TEGPTLTKVK 429 418.5900 1252.7481 1252.5506 158 0 8 10 1 QDSLAQCADFR 750 590.3000 2357.1709 2357.1360 14.8 1 8 5.9 1 GPAGAGPGPAPGTGTAPEPRSESPSR 787 965.8111 2894.4115 2894.4865 -25.90 1 8 6.8 1 MCTPIRGLLMALAVMFGTAMAFAPIPR + Oxidation (M) 713 521.1123 2080.4201 2080.0589 174 0 8 12 1 APQLPGTSEPSAYSPGGLVPR 537 718.9007 1435.7869 1435.7064 56.1 1 8 9.7 1 SPKRPFQCSVCGK 274 523.2287 1044.4429 1044.5564 -108.65 1 8 12 1 DLVQRETGK 403 611.3083 1220.6020 1220.6699 -55.67 1 8 10 1 MSLPIGIYRR + Oxidation (M) 306 537.2905 1072.5665 1072.4785 82.0 0 8 12 1 ADSPAGPESSR 449 641.2889 1280.5632 1280.6837 -94.09 1 8 9.8 1 ELGDHVTNLRK 16 400.2187 798.4228 798.4348 -14.96 1 8 11 1 AAEGRAPK 154 463.2807 924.5469 924.5141 35.4 0 8 9.7 1 RPLETGPR 748 581.3038 2321.1861 2321.1725 5.85 1 8 6.8 1 MKGDYVLGGLFPLGEAEEAGLR 52 419.1999 836.3852 836.3889 -4.40 0 8 11 1 GGAHPGEGR 118 447.2599 892.5053 892.4402 72.9 1 8 12 1 YPRESNK 378 590.3000 1178.5854 1178.5424 36.5 0 8 11 1 CSQPMVGVSGK + Oxidation (M); Propionamide (C) 225 505.2346 1008.4546 1008.4481 6.46 1 8 11 1 VCKGDMGGAR + Oxidation (M) 742 761.7364 2282.1873 2282.2133 -11.38 1 7 7.1 1 QALTIVGTLPFTYMLEKWR + Oxidation (M) 239 509.2445 1016.4744 1016.5801 -103.91 1 7 13 1 AGKTGLAMLR 23 403.2412 804.4678 804.4051 77.9 0 7 13 1 MEGVELK 265 520.2790 1038.5435 1038.4764 64.6 1 7 12 1 KATEGSGSMR + Oxidation (M) 761 635.0799 2536.2904 2536.3571 -26.29 1 7 6.5 1 VNPSRLPVVIGGLLDVDCSEDVIK 643 880.9395 1759.8645 1759.8814 -9.63 0 7 9.2 1 LEWLTLMPNASNLDK + Oxidation (M) 286 529.2750 1056.5354 1056.4481 82.7 0 7 12 1 MHPEEMQR 308 540.3121 1078.6097 1078.5519 53.6 1 7 11 1 GHRAGLPEDK 313 543.2651 1084.5156 1084.5876 -66.42 0 7 12 1 EQLQADLLR 45 416.2976 830.5806 830.4432 165 0 7 14 1 MGNLALGR 355 573.3298 1144.6451 1144.5005 126 0 7 12 1 ANFSIGSMMR + 2 Oxidation (M) 187 480.3523 958.6901 958.4113 291 0 7 13 1 ECVANHMR 773 862.7715 2585.2928 2585.3240 -12.06 1 7 6.9 1 MAATSLMSALAARLLQPAHSCSLR + Oxidation (M); Propionamide (C) 11 593.2000 592.1927 592.3003 -181.56 0 7 13 1 LMASR + Oxidation (M) 188 481.2962 960.5779 960.5062 74.6 1 7 14 1 GCGLNKLEK

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116 446.2561 890.4977 890.4895 9.12 1 7 14 1 ATKTPMVK + Oxidation (M) 811 1091.0000 4359.9709 4361.2518 -293.69 0 7 8.6 1 NLTPLEAQEFALHLINSNLQLETAELALWMIWNGTPVR + Oxidation (M) 210 495.2223 988.4300 988.4825 -53.08 1 7 14 1 AEQAEADKK 342 561.3960 1120.7773 1120.5547 199 0 7 13 1 LPSISSTTCGR 292 533.2588 1064.5031 1064.4808 20.9 0 7 12 1 MALSGEGQEK + Oxidation (M) 356 574.2771 1146.5397 1146.5894 -43.38 1 7 13 1 VYSSGRHSVR 222 503.2653 1004.5160 1004.4961 19.8 0 7 14 1 MVGLDIDSR 803 1091.0000 3269.9782 3269.6316 106 0 7 10 1 NALLLLADDGGFESGAYNNSAIATPHLDALAR 275 523.2525 1044.4905 1044.5352 -42.85 0 7 13 1 VGYDVSHIR 745 767.0591 2298.1555 2298.1646 -3.99 1 7 8.1 1 YNCILTNSRVMNIGLGVLMR + 2 Oxidation (M) 98 437.2746 872.5346 872.4352 114 0 7 16 1 GAAPGSQTGK 737 1122.6006 2243.1865 2243.1038 36.9 1 7 8.1 1 NGTVMAPDLPEMLDLAGTRSR 416 619.8803 1237.7461 1237.6092 111 0 7 12 1 QPVGFVSFDSR 632 858.4282 1714.8418 1714.7477 54.9 1 7 11 1 QTWMNNMMKTSEAK + Oxidation (M) 428 627.3220 1252.6295 1252.6299 -0.35 0 7 12 1 EDHEVEVGVLK 212 495.2981 988.5816 988.4583 125 0 7 14 1 VGMTVHCR + Oxidation (M); Propionamide (C) 605 821.4329 1640.8513 1640.8304 12.7 1 7 10 1 RDMAGASGGVAAPLPQK + Oxidation (M) 359 577.2918 1152.5690 1152.5961 -23.57 0 7 12 1 VFMSAASVGIR + Oxidation (M) 521 706.3694 1410.7243 1410.6305 66.5 0 7 11 1 QMLEICESCIR + Oxidation (M); Propionamide (C) 15 400.2168 798.4190 798.4851 -82.72 0 7 13 1 IPLETVK 14 400.2149 798.4153 798.3218 117 0 7 13 1 AFCESDK 560 503.2907 1506.8504 1506.7972 35.3 0 7 11 1 LLAAGVVSAMVCMVK + Oxidation (M) 409 614.3505 1226.6864 1226.6116 61.0 1 7 12 1 QQTGGAPEGRAR 70 428.1886 854.3626 854.5450 -213.46 1 7 11 1 ALLLGRGR 66 425.7626 849.5107 849.3651 171 0 7 16 1 DTCGANAK + Propionamide (C) 384 595.2892 1188.5638 1188.6536 -75.57 0 7 14 1 SLLIQCQLSGK 788 726.1022 2900.3797 2900.1655 73.9 1 7 7.9 1 CKYDTCNCQNNEDCLCAALSSYAR + 3 Propionamide (C) 310 541.2471 1080.4797 1080.5750 -88.16 1 7 13 1 GSFGKVMLAR + Oxidation (M) 169 472.2239 942.4333 942.4633 -31.86 0 7 13 1 AFVCAFSK + Propionamide (C) 17 400.2660 798.5175 798.5075 12.4 1 7 14 1 AARLVAAK 283 527.2658 1052.5171 1052.5727 -52.80 0 7 13 1 LNGSHLGSLR 677 649.1000 1944.2782 1943.9557 166 0 7 11 1 GAVPGTSAAAAVAICQLCDR + Propionamide (C) 574 522.9301 1565.7686 1565.8599 -58.36 1 7 11 1 LNATLGMTVKVTYR 114 445.3233 888.6320 888.4487 206 0 7 17 1 MTPGQALR + Oxidation (M) 309 540.3165 1078.6185 1078.5151 95.9 0 6 14 1 MLCAGSLEGK + Propionamide (C) 601 815.4246 1628.8347 1628.7902 27.3 1 6 12 1 GYLAAIKGMQEASMK + 2 Oxidation (M) 620 420.1000 1676.3709 1675.9080 276 1 6 12 1 VVSHPSGVLELHMKK + Oxidation (M) 769 857.4334 2569.2784 2569.1255 59.5 1 6 7.8 1 ILMCPEHETERINMYCELCR + Oxidation (M); Propionamide (C) 540 724.3672 1446.7199 1446.6554 44.6 0 6 13 1 LFTPEEYEEYK 752 593.2000 2368.7709 2368.1416 266 1 6 16 1 EDRVQTVSMHGLMHPNGAVFK + Oxidation (M) 28 406.2445 810.4745 810.4096 80.0 1 6 12 1 VEHDRR 68 427.2000 852.3854 852.4454 -70.28 0 6 13 1 QAPTSPPR 371 585.3526 1168.6907 1168.6274 54.2 0 6 14 1 LLHNVLMADK + Oxidation (M) 542 483.9065 1448.6978 1448.6718 17.9 0 6 13 1 SPQNQYPAELMR + Oxidation (M) 616 417.2000 1664.7709 1664.8960 -75.11 0 6 12 1 LYPLAIQICEYLR + Propionamide (C)

Downloaded from iovs.arvojournals.org on 09/26/2021 17 of 23 8/06/2012 1:09 PM Peptide Summary Report (Submitted from PROJ13880 LingZhu by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120608/F077324.dat&REPTYPE=pe...

753 793.0659 2376.1759 2376.2024 -11.14 0 6 9.1 1 FNQYIVCLAHHVIAMWFIR + Oxidation (M) 729 724.3570 2170.0493 2170.1391 -41.37 1 6 9.5 1 VAVEAFQICCLLLPPENRR 242 511.2340 1020.4535 1020.4949 -40.57 1 6 16 1 RTSSGGGGGTGK 394 402.9012 1205.6818 1205.6438 31.5 1 6 14 1 KTNIIDSMLR + Oxidation (M) 663 455.2000 1816.7709 1816.9254 -85.02 0 6 12 1 ALEQLNGFELAGRPMR + Oxidation (M) 672 642.3266 1923.9581 1923.8091 77.4 0 6 11 1 SIHTGDRPYECSECEK + Propionamide (C) 610 551.9076 1652.7011 1652.9250 -135.44 1 6 12 1 ILLKSVLDQWSSHK 67 426.2195 850.4245 850.4331 -10.09 1 6 14 1 MKTTSQR 520 470.5848 1408.7326 1408.8150 -58.51 1 6 13 1 LRLSVSSHILER 530 475.9131 1424.7174 1424.6136 72.8 1 6 13 1 ELNMGQRCSDTR + Oxidation (M) 75 429.1954 856.3762 856.4879 -130.40 1 6 16 1 APRGTLSR 266 521.2678 1040.5211 1040.6454 -119.51 1 6 14 1 LLSIVGRQR 271 522.2813 1042.5481 1042.6135 -62.73 0 6 16 1 AVAVAALSVSR 657 597.9396 1790.7971 1790.8695 -40.44 1 6 13 1 KINMSGYILFSSEMR + Oxidation (M) 214 496.7899 991.5652 991.5702 -5.09 0 6 17 1 IANLAYVTK 340 561.2884 1120.5622 1120.6002 -33.95 1 6 17 1 RHRPQHYK 282 525.3472 1048.6799 1048.4785 192 0 6 17 1 NSVTSQEER 375 587.2906 1172.5667 1172.5898 -19.70 1 6 17 1 AESGRQQLER 321 550.2908 1098.5671 1098.5532 12.7 0 6 16 1 AYCNLGLAFK 480 445.9244 1334.7514 1334.6652 64.6 1 6 15 1 THYRLELMEK + Oxidation (M) 599 813.9179 1625.8213 1625.7252 59.1 0 6 13 1 MNTLQAIWMMDPK + 3 Oxidation (M) 113 445.2414 888.4683 888.4301 43.1 1 6 20 1 VEAEASRQ 731 727.7066 2180.0978 2180.0401 26.5 1 6 10 1 WESAYVWCISGTNKVYFK 180 477.2121 952.4096 952.4614 -54.34 0 6 15 1 ESLDAHPGK 229 505.2814 1008.5482 1008.4698 77.7 0 6 16 1 KPEMADFR + Oxidation (M) 680 650.9945 1949.9615 1949.9429 9.54 0 6 12 1 VTAEADSSSPTGILATSESK 717 707.3428 2119.0067 2119.1161 -51.63 1 6 11 1 SEAEANTQVTLVKTFAALNI 675 486.2000 1940.7709 1940.9522 -93.41 1 6 12 1 MTVIVQEMGGDRLAFMK + Oxidation (M) 18 402.2274 802.4402 802.4007 49.2 0 6 22 1 IPAEMAR + Oxidation (M) 667 628.3223 1881.9452 1881.9870 -22.19 1 6 12 1 MKEPTQVEALHTEILK + Oxidation (M) 758 624.3140 2493.2270 2493.2335 -2.62 1 6 10 1 NNRPHASYEPELHPAVCYRIK 258 517.2602 1032.5059 1032.4777 27.3 1 6 20 1 HYWKDER 218 499.2346 996.4547 996.5716 -117.33 1 6 17 1 NVPLGKDVR 334 557.2568 1112.4990 1112.6553 -140.50 1 5 17 1 VEQKIEVIR 482 671.3374 1340.6603 1340.6394 15.6 1 5 17 1 SALRDAYCSEVK 705 519.1295 2072.4890 2073.0127 -252.64 1 5 22 1 ADEDLIFRLEGVDGGQSPR 807 928.8967 3711.5577 3711.9053 -93.66 1 5 13 1 MEMFTFLLTCIFLPLLRGHSLFTCEPITVPR + Oxidation (M); Propionamide (C) 83 431.2283 860.4419 860.4789 -42.99 0 5 22 1 IMLELAR + Oxidation (M) 97 437.2291 872.4436 872.5120 -78.35 0 5 24 1 LWIAATAK 317 545.3044 1088.5943 1088.5648 27.1 0 5 20 1 NGTNVACLVK + Propionamide (C) 456 648.3107 1294.6068 1294.5143 71.5 1 5 16 1 GNCTRGENDCR + Propionamide (C) 151 463.2257 924.4369 924.4413 -4.79 0 5 16 1 DLNSPHSR 493 681.3633 1360.7120 1360.7173 -3.92 1 5 18 1 AGPQPCYLKLGSK 397 605.3070 1208.5995 1208.5893 8.42 1 5 18 1 TVCTLNMGDKK 720 1063.5104 2125.0062 2125.1201 -53.60 0 5 13 1 LACLIGVEGGHSLDSSLSVLR

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726 719.0225 2154.0457 2154.1685 -57.01 0 5 13 1 LPPIVHLPENIGVDTPTQSK 27 406.2274 810.4402 810.4824 -52.08 1 5 16 1 ALPGARAR 192 485.2477 968.4808 968.4208 62.0 0 5 18 1 CPYGVTCR + Propionamide (C) 801 773.1367 3088.5178 3089.4263 -294.07 0 5 13 1 CVEHAYEPTPDDVAISYLPLAHMFER + Oxidation (M); Propionamide (C) 130 455.2000 908.3854 908.5192 -147.20 1 5 19 1 HSALKTPR 763 637.8150 2547.2309 2547.2574 -10.40 1 5 11 1 TSAAMLSQQVASVKWPNSVMAPGR + 2 Oxidation (M) 165 471.2130 940.4115 940.4337 -23.71 0 5 18 1 WHGPAMAR + Oxidation (M) 452 430.8826 1289.6258 1289.7568 -101.52 1 5 18 1 TGPVLAKLHQAR 723 715.0225 2142.0457 2142.0858 -18.69 1 5 14 1 EPPNPEYQEFQNRLLIR 48 417.2033 832.3921 832.5058 -136.57 0 5 23 1 AEILFLK 24 404.2220 806.4295 806.4398 -12.81 1 5 22 1 ARYLER 315 544.2887 1086.5628 1086.5921 -26.89 1 5 23 1 ELSKDSPALK 211 495.2518 988.4890 988.4978 -8.90 0 5 23 1 YLSLHDNK 767 640.8242 2559.2676 2559.4135 -56.98 0 5 12 1 SAAFFVIGASLGEMAIPAVIGILQGK 160 469.2088 936.4030 936.5029 -106.68 0 5 20 1 DKPPQPGAK 764 852.4267 2554.2582 2554.3386 -31.48 1 5 13 1 TAVPPKTIEECEVILMVGLPGSGK + Oxidation (M); Propionamide (C) 280 525.2367 1048.4588 1048.5223 -60.57 1 5 22 1 DCSQDLLKK 339 561.2704 1120.5263 1120.5302 -3.45 0 5 23 1 GFHFTVDGNK 415 413.1000 1236.2782 1236.5986 -259.14 0 5 14 1 YGTELNQGDLK 692 497.2370 1984.9188 1984.9420 -11.69 1 5 14 1 MECPVMETGSLFTSGIKR 57 421.2109 840.4073 840.4566 -58.65 1 5 18 1 APERVGGR 102 441.2063 880.3980 880.3749 26.3 0 5 21 1 HTSMYDK 450 428.5626 1282.6658 1282.6881 -17.34 1 5 18 1 ESRGEIIPPASK 50 417.2188 832.4230 832.3974 30.8 1 5 25 1 MPSGRGGR + Oxidation (M) 191 485.2289 968.4433 968.4498 -6.68 1 5 20 1 MYSAEGRR 166 471.2397 940.4648 940.4913 -28.08 0 5 20 1 MTPARPPR + Oxidation (M) 285 528.2786 1054.5427 1054.5083 32.6 0 5 20 1 EFSEQFIR 249 513.2745 1024.5345 1024.4938 39.8 0 5 20 1 GSGSSSYRPK 568 769.3141 1536.6136 1536.5587 35.8 0 5 18 1 NEEEEEEEDEEK 618 836.4271 1670.8396 1670.9694 -77.68 1 5 17 1 MAFVKHLLLFLSPR 170 472.2835 942.5525 942.5208 33.6 0 5 22 1 APEILLGCK 157 467.2108 932.4071 932.5001 -99.73 0 4 26 1 CPTSLSVVK 190 483.2387 964.4629 964.5342 -73.88 0 4 25 1 LYVSGTGLR 458 432.8801 1295.6183 1295.7422 -95.60 1 4 21 1 GAAGGLGLAGGRALR 215 497.2517 992.4889 992.6382 -150.39 1 4 24 1 AGLAKVIPPK 194 486.2000 970.3854 970.5923 -213.15 1 4 23 1 ALANVISRK 810 1383.8853 4148.6341 4148.8042 -41.00 1 4 14 1 RCIEDLPAACMLGPEGVPVSWVTMDPSCEVGMAYSMEK + 3 Oxidation (M) 652 593.2000 1776.5782 1776.8424 -148.73 0 4 21 1 DTATLNCQSSGSKPAAR + Propionamide (C) 420 623.3216 1244.6287 1244.6738 -36.24 0 4 23 1 LNSHLIQHQR 63 425.2084 848.4023 848.4392 -43.56 0 4 26 1 DPTLTFR 401 405.5812 1213.7219 1213.5615 132 0 4 24 1 NIFGSDEAFSK 145 460.2679 918.5212 918.4229 107 0 4 28 1 NALGCENK + Propionamide (C) 476 666.3341 1330.6536 1330.7292 -56.83 1 4 22 1 GFAERIRPMVR 20 402.2349 802.4553 802.4297 32.0 0 4 30 1 ATSGAAGLR 244 511.2575 1020.5005 1020.4957 4.73 1 4 26 1 CATQLCRR + Propionamide (C)

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470 656.3059 1310.5973 1310.6102 -9.88 0 4 22 1 QLSYSDNQASAK 467 436.5212 1306.5417 1306.7609 -167.72 0 4 22 1 SVPEGPVQLLIR 85 433.2088 864.4031 864.4197 -19.22 1 4 23 1 ELCKMNK 464 435.7902 1304.3486 1304.6911 -262.47 1 4 20 1 NWAAIAKMVGTK + Oxidation (M) 29 406.2464 810.4783 810.3290 184 0 4 21 1 SCSSQSGR 176 475.2199 948.4252 948.5393 -120.27 0 4 27 1 TAVIGAVYR 319 548.3086 1094.6027 1094.5827 18.2 1 4 22 1 SLMAKLSSMK 92 435.2906 868.5667 868.3233 280 0 4 20 1 MDDSTER + Oxidation (M) 433 420.1000 1257.2782 1257.6023 -257.75 0 4 14 1 TEPLISPGCDR + Propionamide (C) 203 491.2408 980.4671 980.4385 29.1 0 4 24 1 NLCEEFAR 221 503.2419 1004.4693 1004.4485 20.8 0 4 27 1 AGEVICIDD + Propionamide (C) 31 407.2138 812.4130 812.3963 20.5 1 4 23 1 KNCPSHK 533 714.3725 1426.7305 1426.6650 45.9 0 4 23 1 DIDLATTMFEAGK + Oxidation (M) 549 492.2163 1473.6271 1473.7068 -54.09 0 4 22 1 AGAAAGGPGVSGVCVCK + Propionamide (C) 77 430.2171 858.4196 858.4923 -84.62 1 4 33 1 ASGQAIGKK 770 857.7465 2570.2176 2570.2549 -14.54 0 4 15 1 GSIDALFNMTPPTSALGACFVFSPK 641 877.9096 1753.8046 1753.7361 39.1 0 4 21 1 MDAPDGCPPAVYEVMK + 2 Oxidation (M) 200 489.2258 976.4370 976.4825 -46.63 0 4 31 1 ELSDISSAR 259 517.8112 1033.6078 1033.4928 111 0 4 29 1 QEVEDSVTK 629 857.4002 1712.7858 1712.7941 -4.83 1 4 21 1 EGPWEVGGRSEHVMK + Oxidation (M) 702 1030.0020 2057.9895 2058.0528 -30.77 1 4 19 1 VCDNDTVPSVSSINRIIR + Propionamide (C) 153 463.2542 924.4939 924.4409 57.4 1 4 24 1 DLQKMMK + 2 Oxidation (M) 290 532.3198 1062.6251 1062.6073 16.8 0 4 28 1 LVNYQISVK 709 519.1468 2072.5579 2072.1167 213 1 4 33 1 TPSVPSVPHELPRVPSFAR 312 543.2144 1084.4142 1084.5301 -106.92 1 4 29 1 AFGKNNSYGK 466 653.8386 1305.6626 1305.6565 4.69 0 4 24 1 DFQAISDVIGNK 294 533.2741 1064.5337 1064.5284 4.93 1 4 28 1 ACRIDSTTK + Propionamide (C) 56 421.2051 840.3956 840.3912 5.27 0 4 23 1 CHVPETR 505 461.9052 1382.6938 1382.6314 45.2 0 3 27 1 SPPSTGSTYGSSQK 84 432.2504 862.4863 862.3967 104 0 3 34 1 EMGQNLR + Oxidation (M) 230 506.3069 1010.5992 1010.5080 90.3 1 3 29 1 APHMIRDR + Oxidation (M) 36 412.7496 823.4847 823.4916 -8.31 0 3 27 1 VDPIKPR 136 457.2062 912.3979 912.4413 -47.57 0 3 29 1 GLQESSHR 119 449.2007 896.3869 896.3810 6.53 0 3 25 1 AFMDGSNR 412 617.2944 1232.5743 1232.5141 48.9 0 3 29 1 MLWSCTFCR + Oxidation (M); Propionamide (C) 358 576.3284 1150.6422 1150.5506 79.6 0 3 31 1 EDQVGAEYLK 247 513.2210 1024.4274 1024.5124 -82.98 1 3 28 1 CRNTIGSFK 256 517.2473 1032.4801 1032.5175 -36.20 0 3 35 1 YTVIMNHR 41 415.1000 828.1854 828.4090 -269.82 0 3 11 1 ATPSPNSR 728 722.3673 2164.0801 2164.1964 -53.72 1 3 20 1 VNLLEQELQELRAQAALAR 162 469.2436 936.4726 936.4552 18.6 0 3 31 1 GYASPDLSK 351 571.2816 1140.5487 1140.4917 50.0 0 3 30 1 THHCSICNR + Propionamide (C) 232 507.2461 1012.4776 1012.4865 -8.83 0 3 30 1 EAFQIYDK 132 455.2320 908.4495 908.4426 7.65 1 3 32 1 KGMDFAPK + Oxidation (M) 49 417.2130 832.4114 832.4477 -43.51 1 3 38 1 MKNPVTK + Oxidation (M)

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181 477.2348 952.4551 952.5277 -76.18 0 3 31 1 VHCGVLGLR 183 479.2251 956.4356 956.4749 -41.18 0 3 34 1 HPSLATMGK + Oxidation (M) 195 486.2652 970.5158 970.5923 -78.83 1 3 33 1 VVKAASGALR 164 471.2007 940.3868 940.5202 -141.88 1 3 30 1 RAPAGGSLGR 206 493.2411 984.4676 984.4811 -13.64 0 3 32 1 MAAAPQAPGR + Oxidation (M) 331 556.3058 1110.5971 1110.6258 -25.83 1 3 29 1 RPGARTGPATK 25 405.2060 808.3974 808.3637 41.8 0 3 40 1 VGEMETK + Oxidation (M) 61 423.2248 844.4351 844.4151 23.7 1 3 43 1 GAQGRNDK 273 522.8674 1043.7202 1043.5433 169 0 3 36 1 GSSHLCSLLK 207 493.2726 984.5307 984.4083 124 0 3 34 1 GSYGNGGAMR + Oxidation (M) 110 443.2116 884.4087 884.3237 96.1 1 3 32 1 CCCCRR + 2 Propionamide (C) 715 699.0141 2094.0205 2093.9663 25.9 0 3 24 1 ASLWLFLSSEEQMCGTHR 281 525.3429 1048.6713 1048.5302 135 0 3 38 1 TPPEGPQAPR 159 467.3478 932.6811 932.5551 135 1 2 40 1 LILIKCCK 263 519.2977 1036.5808 1036.5045 73.6 0 2 35 1 SCSVLQMAK + Propionamide (C) 734 555.2999 2217.1707 2217.0258 65.4 1 2 22 1 SREEGEQGPSSGASSQVLEQR 104 441.2182 880.4219 880.5243 -116.26 1 2 35 1 SKLQIHR 406 613.2864 1224.5582 1224.5631 -4.02 0 2 33 1 CFQMEEAVLR 141 458.2446 914.4746 914.4947 -22.01 1 2 42 1 ARAHPHAR 712 693.3578 2077.0516 2076.9107 67.8 0 2 26 1 MMAFCYQILTDPNFDPR + Oxidation (M) 267 521.2700 1040.5254 1040.5614 -34.63 1 2 33 1 AALRSPDSPK 226 505.2410 1008.4674 1008.4845 -16.95 1 2 34 1 MAASGRGLCK + Oxidation (M) 58 421.2109 840.4073 840.3734 40.3 1 2 31 1 NCFRCAK 163 469.2477 936.4808 936.4586 23.7 0 2 35 1 LETATCTAK 35 411.1789 820.3433 820.4000 -69.09 0 2 40 1 CELESLK 125 450.2701 898.5256 898.4807 49.9 0 2 33 1 GLGMRPPR + Oxidation (M) 19 402.2274 802.4402 802.4912 -63.63 1 2 51 1 ISALKGSK 38 414.2136 826.4126 826.4007 14.4 0 2 37 1 TFCSTLR 150 463.2217 924.4288 924.4011 29.9 0 2 38 1 WGSICATST 72 428.2571 854.4996 854.4610 45.2 0 2 35 1 NHTISVGK 348 569.2777 1136.5409 1136.5132 24.4 0 2 44 1 ACSETLAESR + Propionamide (C) 103 441.2123 880.4100 880.5382 -145.61 1 2 42 1 APEVKLPK 12 649.1000 648.0927 648.2867 -299.28 0 1 5.4 1 DRPDF 184 479.2313 956.4480 956.4386 9.86 1 1 49 1 YTMEGKGR + Oxidation (M) 82 430.2976 858.5806 858.3952 216 1 1 60 1 AARCHACK 304 537.2576 1072.5007 1072.5989 -91.54 1 1 54 1 IRTEASQIR 101 439.7231 877.4317 877.4440 -13.94 1 1 56 1 MEATRVR + Oxidation (M) 53 420.1000 838.1854 838.3834 -236.16 0 1 25 1 HFDAHGR 297 534.3123 1066.6101 1066.5481 58.1 1 1 47 1 CFTPKGSSLK 353 572.3291 1142.6436 1142.5792 56.3 1 1 56 1 AETRASANAPR 81 430.2760 858.5374 858.4811 65.6 0 1 66 1 LEQGTLAK 248 513.2403 1024.4660 1024.6142 -144.64 1 1 47 1 VASALVPGRR 78 430.2171 858.4196 858.5215 -118.60 0 1 70 1 FLELLPK 156 464.8316 927.6486 927.4120 255 0 1 55 1 DYSLTCR + Propionamide (C) 179 476.2681 950.5217 950.5185 3.30 1 0 59 1 VKLGTDYR

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108 442.2482 882.4818 882.4923 -11.88 0 0 50 1 LGSPKPER 261 519.1403 1036.2660 1036.4608 -187.86 0 0 49 1 TSQLDNGMR + Oxidation (M) 238 509.2274 1016.4403 1016.5338 -91.99 1 0 66 1 IYAVCHRR 120 449.2248 896.4350 896.3480 97.1 0 0 52 1 CQSDAAMR + Oxidation (M) 121 449.2388 896.4631 896.4828 -21.97 1 0 52 1 AHEVASKR 1 413.1000 412.0927 2 415.1000 414.0927 3 415.2000 414.1927 4 415.2115 414.2043 5 417.2000 416.1927 6 420.1000 419.0927 7 427.2000 426.1927 8 455.2000 454.1927 9 486.2000 485.1927 10 590.3000 589.2927 33 409.2035 816.3925 60 422.1667 842.3189 65 425.2143 848.4140 69 427.2005 852.3865 71 428.2160 854.4174 88 434.2333 866.4521 90 435.2354 868.4562 100 439.1000 876.1854 107 442.2104 882.4062 112 444.2778 886.5410 131 455.2098 908.4051 137 457.2224 912.4303 140 458.2385 914.4624 143 458.7895 915.5644 146 461.2406 920.4666 148 462.2469 922.4792 152 463.2420 924.4695 158 467.3172 932.6198 182 477.2720 952.5295 205 491.7358 981.4570 268 521.2721 1040.5297 298 535.2268 1068.4391 318 1091.0000 1089.9927 324 551.3455 1100.6764 382 593.2000 1184.3854 459 649.1000 1296.1854 498 457.2730 1368.7972 569 770.3955 1538.7765 730 1091.0000 2179.9854

Downloaded from iovs.arvojournals.org on 09/26/2021 22 of 23 8/06/2012 1:09 PM Peptide Summary Report (Submitted from PROJ13880 LingZhu by Mascot Daemon on APAF-WS-08) http://192.168.1.222/mascot/cgi/master_results.pl?file=../data/20120608/F077324.dat&REPTYPE=pe...

Search Parameters

Type of search : MS/MS Ion Search Enzyme : Trypsin Variable modifications : Oxidation (M),Propionamide (C) Mass values : Monoisotopic Protein Mass : Unrestricted Peptide Mass Tolerance : ± 300 ppm Fragment Mass Tolerance: ± 0.6 Da Max Missed Cleavages : 1 Instrument type : ESI-QUAD-TOF Number of queries : 811

Mascot: http://www.matrixscience.com/

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