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B- Poster Presentations Thyroid Disease Vs Abstracts of 15th Congress of Iranian Society for Reproductive Medicine Results: In control group, there were 4 cases of B- Poster Presentations thyroid disease vs. 0 in case group (P=0.02). The mean average of ANA, dsDNA, RF, anti 1- Infertility, Gynecology thyroglobulin Ab, anti TPO (anti microsomal Ab) and TSH didn’t have any significant differences P-1 between two groups. Evaluation of the thyroid autoimmunity in Conclusion: Based on our study, we couldn’t find POF women in comparison with normal any disturbances either in immunological tests or ovulatory women in thyroid function of POF women as compared with normal ovulatory women. 1 2 Mohammadzadeh A , Jeddi-Tehrani M , Arefi Key words: POF, Anti TPO, TSH, Immunology. 1 3 4 S , Soltanghoraee H , Zeraati H . 1 Department of Reproductive Endocrinology and P-2 Embryology, Research Center of Reproductive Comparative molecular and culture-based Biotechnology, Aviccena Research Institute, ACERCR, diagnosis of vaginal colonization by group B Tehran, Iran. streptococcus in pregnant women during 2 Department of Monoclonal Antibodies, Research labor Center of Monoclonal Antibodies, Aviccena Research Institute, ACERCR, Tehran, Iran. Fatemi F1, Chamani-Tabriz L2, Pakzad P1, 3 Department of Reproductive and Biotechnology and Zeraati H3, Rabbani H4. Genetics, Research Center of Reproductive 1 Department of Microbiology, Islamic Azad University, Biotechnology, Aviccena Research Institute, ACERCR, North Tehran Branch, Tehran, Iran. Tehran, Iran. 2 Research Center of Reproductive Biotechnology, 4 Department of Epidemiology and Biostatistics, Avicenna Research Institute, ACECR, Tehran, Iran. Faculty of Medical Sciences, Tehran University of 3 Department of Epidemiology and Biostatistics, School Medical Sciences, Tehran, Iran. of Public Health, Tehran University of Medical E-mail: af85af @yahoo.com Sciences, Tehran, Iran. 4 Research Center of Nanobiotechnology, Avicenna Introduction: Premature ovarian failure (POF) Research Institute, ACECR, Tehran, Iran. occurs in women younger than 40 with 1-5 % prevalence. POF is a pathological condition and Introduction: Group B Streptococci (GBS) or has negative effect on the health of affected Streptococcus agalactiae are members of the women and leads to infertility. The main etiology normal flora of the female genital tract and are an of this disease is unknown but in many studies, important etiological agent of neonatal sepsis and immunological disturbances especially against meningitis. thyroid glands have been introduced as possible Rapid detection methods for GBS may be causes. advantageous for women who present in labor However, it hasn’t been proven by other studies. without any information on their GBS colonization The aim of this study is the evaluation of several status and allow early treatment of neonates. This immunological tests in the POF women in study aimed to evaluate the diagnostic accuracy of comparison with normal ovulatory women. PCR assay for detection of group B Streptococcus Materials and Methods: This prospective clinical using a specific culture method. case-control trial was done in Avicenna Infertility Materials and Methods: Two swabs were used to Clinic on 30 nonsmoking women with spontaneous obtain vaginal specimen from 330 pregnant POF at least for 1 year who didn’t have any women attending delivery room at Hedayat previous history of bilateral oophorectomy, Hospital, Tehran, Iran, from April through July chemotherapy and radiotherapy with the serum rd 2008. A swab was immediately placed into Amies FSH 3 level more than 30U/L (case group) and transport medium with charcoal (Hi-Media, India) compared with 30 nonsmoking normal ovulatory and another swab was soaked in tube containing 1 women (<40 years.old) who were under infertility ml of phosphate-buffered saline (PBS), (pH 7.2). workup for male factor with serum FSH level One swab was analyzed using a specific culture lower than 8 U/L (control group). The below stated method, which included direct plating onto tests were done in each group: ANA, dsDNA, RF, selective GBS agar medium (Islam). S. agalactiae anti thyroglobulin Ab, anti TPO (anti microsomal developed orange-red pigmented colonies in GBS Ab) and hormonal tests including: FSH-LH-TSH. agar plates under anaerobic condition. The other Iranian Journal of Reproductive Medicine, Vol. 7, Suppl 1, Winter 2009 39 Abstracts of 15th Congress of Iranian Society for Reproductive Medicine swab was used for DNA extraction and PCR assay can inhibit other potentially harmful targeting the S. agalactiae 16S ribosomal RNA microorganisms, prevent infections of the gene for detection of group B Streptococcus. The urogenital tracts and associated with a reduced risk length of the amplified product was 405 bp. To of bacterial vaginosis (BV). We studied pregnant confirm amplicon production, the PCR products women during labor phase to determine the were analyzed by electrophoresis. prevalence of Lactobacillus species and effective Results: Among the 330 pregnant women, 68 factors on vaginal colonization of these bacteria. (20.6%) (95% CI 16.7-24.5%) were identified as Materials and Methods: A total of 330 vaginal carriers of group B Streptococci on the basis of the swabs were collected from women attending results of culture on GBS agar. Both of the culture delivery room at Hedayat Hospital, Tehran, Iran, and PCR methods were positive for 56 (17%) and from April through July 2008. Cotton swabs negative for 253 (76.6%) women. The culture contaminated with vaginal fluid were placed in method was positive and PCR was negative in 12 tubes contained 1 ml phosphate-buffered saline (3.6%) women. The culture was negative and the (PBS), (pH 7.2) and with completed questionnaire PCR positive for 9 (2.7%) women. Sensitivity of forms, transported to the Avicenna laboratory the PCR assay was 82.3% (95% CI 78.4-86.2%) daily. DNA was extracted from the bacteria and specificity was 96.5% (95% CI 94.5-98.4%). present on vaginal swabs and PCR assay was The positive predictive value was 86.15% and carried out by universal primers which uses negative predictive value was 95.4%. amplification to detect the presence of the 16s Conclusion: The results of our study indicate that rRNA gene of Lactobacillus species. The length of the colonization rate of GBS in studied population the amplified product is 231-233 bp. To confirm of pregnant women is relatively high. A rapid amplicon production, the PCR products were method for the detection of this organism at the analyzed by electrophoresis. All data were time of delivery is greatly needed. Group B statistically analyzed by independent t-tests, chi Streptococci can be detected rapidly and reliably square, Fisher’s exact test, and multiple logistic by a PCR assay of vaginal secretion from pregnant models. p<0.05 was considered as significant. women during labor and decrease the time to Results: Among 330 samples, the results of the detection of GBS. PCR were positive for Lactobacilli in 322 women Key words: Group B Streptococcus (GBS), Vaginal flora, (97.6%) (CI 95%: 96.0-99.2%). The mean age of PCR, GBS agar. Lactobacilli positive women was 25.7±4.6 years and the mean age of Lactobacilli negative women P-3 was 30.7±4.5 years. According to their Colonization rate of vaginal Lactobacillus questionnaire data, 78 (92.9%) of 84 with history species in pregnant women during labor of vaginitis during pregnancy, 244 (99.2%) of 246 without history of vaginitis and 7 (77.8%) of 9 Fatemi F1, Chamani-Tabriz L2, Pakzad P1, who delivered < 37 weeks, 315 (98.1%) of 321 Zeraati H3, Rabbani H4. who delivered > 37 weeks, were positive PCR 1 Department of Microbiology, Islamic Azad University, results. Statistically analysis showed significant North Tehran Branch, Tehran, Iran. relationship between the mean age of participants, 2 Research Center of Reproductive Biotechnology, history of vaginitis and gestation age with PCR Avicenna Research Institute, ACECR, Tehran, Iran. results of Lactobacillus species (p<0.05). 3 Department of Epidemiology and Biostatistics, School Conclusion: The results indicate that the of Public Health, Tehran University of Medical prevalence of Lactobacillus species in studied Sciences, Tehran, Iran. population of pregnant women is high. There is 4 Research Center of Nanobiotechnology, Avicenna considerable evidence that the prevalence of Research Institute, ACECR, Tehran, Iran. vaginal Lactobacilli is associated with increased levels of estrogen which increases during Introduction: The human vagina and its pregnancy. Moreover, the colonization rate of microflora form a balanced ecosystem having an Lactobacillus species in younger women, without important role in maintaining the vaginal health of history of vaginitis and term birth is more than women. The vaginal microflora composed of both older women, with history of vaginitis and preterm anaerobic and aerobic bacterial species in which birth (p<0.05). These findings reflect the Lactobacilli are the most predominant bacterial relationship between the age and presence of species in women of reproductive age. Lactobacilli Lactobacillus species in vagina, beneficial role of 40 Iranian Journal of Reproductive Medicine, Vol. 7, Suppl 1, Winter 2009 Abstracts of 15th Congress of Iranian Society for Reproductive Medicine Lactobacilli in term birth at > 37 weeks gestation removed. Visible gonad can be removed with and prevention of vaginal infection. laparoscope, the uterus and tubes should be Key words: Lactobacillus species, Vaginal flora, Vaginitis, preserved for the possibility of pregnancy with Preterm delivery. donor oocytes. If the gonads prove to be inaccessible, preoperative MRI is helpful to P-4 accurately localize gonadal tissue and select the Laparoscopic removal of dysgenetic gonad correct operative approach because the gonads can containing a gonadoblastoma in a patient be close to the external iliac artery and herniated in .with Swyer Syndrome fallowed byَ ART and to the inguinal canals oocyte donation Key words: Gonadectomy, Laparoscopy, Swyer Syndrome, ART. Beigi Sh1, Eimani H2. 1 Baghiyatallah University of Medical Sciences, P-5 Tehran, Iran. Fertility sparing in infertile women with 2 Kowsar IVF Center, Tehran, Iran.
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