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SM568P Monoclonal Antibody to alpha Tubulin / TUBA1B - Purified

Alternate names: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha- ubiquitous chain Quantity: 0.5 mg Concentration: 1.0 mg/ml Background: are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a -associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Uniprot ID: P68363 NCBI: NP_006073 GeneID: 10376 Host / Isotype: Rat / IgG2a Recommended Isotype SM15P, SM15PX Controls: Clone: YOL1/34 Immunogen: Yeast Tubulin. Spleen cells from immunised LOU rats were fused with cells of the rat YB2/0 Myeloma cell line. Format: State: Liquid purified IgG fraction. Purification: Affinity Chromatography on Protein G Buffer System: PBS Preservatives: 0.09% Sodium Azide Applications: ELISA: 10 µg/ml as Detecting antibody. Radioimmunoassay. Western blotting. Immunofluorescence. Flow Cytometry (Not tested). Immunohistochemistry on Frozen and Paraffin Sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: SM568P specifically recognizes the alpha subunit of Tubulin. The reactivity pattern is similar to that seen with Clone YL1/2 (cat.-No SM2202P). This antibody is routinely tested in ELISA on Tubulin.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20191119 1 / 4 SM568P: Monoclonal Antibody to alpha Tubulin / TUBA1B - Purified

Species Reactivity: Tested: Drosophila, Human, Arabidopsis, Seagrass (Cymodocea nodosa), Saccharomyces, Platyzoma, Ashbya, Mouse, Naegleria, Asplenium nidus. Expected from sequence similarity: Birds, Mammals, Fungal. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Product Citations: Purchased from Acris: 1. Schneider L, Essmann F, Kletke A, Rio P, Hanenberg H, Wetzel W, et al. The transforming acidic coiled coil 3 protein is essential for spindle-dependent alignment and mitotic survival. J Biol Chem. 2007 Oct 5;282(40):29273-83. Epub 2007 Aug 5. PubMed PMID: 17675670. 2. Lang A, Grether-Beck S, Singh M, Kuck F, Jakob S, Kefalas A, et al. MicroRNA-15b regulates mitochondrial ROS production and the senescence-associated secretory phenotype through sirtuin 4/SIRT4. Aging (Albany NY). 2016 Mar;8(3):484-509. PubMed PMID: 26959556. General Readings: 1. Kilmartin JV, Wright B, Milstein C. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J Cell Biol. 1982 Jun;93(3):576-82. PubMed PMID: 6811596. 2. Waples WG, Chahwan C, Ciechonska M, Lavoie BD. Putting the brake on FEAR: Tof2 promotes the biphasic release of Cdc14 phosphatase during mitotic exit. Mol Biol Cell. 2009 Jan;20(1):245-55. doi: 10.1091/mbc.E08-08-0879. Epub 2008 Oct 15. PubMed PMID: 18923139. 3. White J, Stead E, Faast R, Conn S, Cartwright P, Dalton S. Developmental activation of the Rb-E2F pathway and establishment of cell cycle-regulated cyclin-dependent kinase activity during embryonic stem cell differentiation. Mol Biol Cell. 2005 Apr;16(4):2018-27. Epub 2005 Feb 9. PubMed PMID: 15703208. 4. Lang C, Grava S, Finlayson M, Trimble R, Philippsen P, Jaspersen SL. Structural mutants of the spindle pole body cause distinct alteration of cytoplasmic microtubules and nuclear dynamics in multinucleated hyphae. Mol Biol Cell. 2010 Mar 1;21(5):753-66. doi: 10.1091/mbc.E09-07-0555. Epub 2010 Jan 6. PubMed PMID: 20053682. 5. Hao, N. et al. (2011), Nucleic Acids Res. Jan 17. [Epub ahead of print] 6. D'Ambrosio C, Schmidt CK, Katou Y, Kelly G, Itoh T, Shirahige K, et al. Identification of cis-acting sites for condensin loading onto budding yeast . Dev. 2008 Aug 15;22(16):2215-27. doi: 10.1101/gad.1675708. PubMed PMID: 18708580. 7. Hartl TA, Sweeney SJ, Knepler PJ, Bosco G. Condensin II resolves chromosomal associations to enable anaphase I segregation in Drosophila male meiosis. PLoS Genet. 2008 Oct;4(10):e1000228. doi: 10.1371/journal.pgen.1000228. Epub 2008 Oct 17. PubMed PMID: 18927632. 8. Mirchenko L, Uhlmann F. Sli15(INCENP) dephosphorylation prevents mitotic checkpoint reengagement due to loss of tension at anaphase onset. Curr Biol. 2010 Aug 10;20(15):1396-401. doi: 10.1016/j.cub.2010.06.023. Epub 2010 Jul 8. PubMed PMID: 20619650. 9. Sullivan M, Lehane C, Uhlmann F. Orchestrating anaphase and mitotic exit:

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20191119 2 / 4 SM568P: Monoclonal Antibody to alpha Tubulin / TUBA1B - Purified

separase cleavage and localization of Slk19. Nat Cell Biol. 2001 Sep;3(9):771-7. PubMed PMID: 11533655. 10. Sullivan M, Holt L, Morgan DO. Cyclin-specific control of ribosomal DNA segregation. Mol Cell Biol. 2008 Sep;28(17):5328-36. doi: 10.1128/MCB.00235-08. Epub 2008 Jun 30. PubMed PMID: 18591250. 11. Yu HG, Koshland D. The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis. J Cell Biol. 2007 Mar 26;176(7):911-8. Epub 2007 Mar 19. PubMed PMID: 17371833. 12. Rossio V, Yoshida S. Spatial regulation of Cdc55-PP2A by Zds1/Zds2 controls mitotic entry and mitotic exit in budding yeast. J Cell Biol. 2011 May 2;193(3):445-54. doi: 10.1083/jcb.201101134. PubMed PMID: 21536748. 13. Grava S, Keller M, Voegeli S, Seger S, Lang C, Philippsen P. Clustering of nuclei in multinucleated hyphae is prevented by -driven bidirectional nuclear movements and microtubule growth control in Ashbya gossypii. Eukaryot Cell. 2011 Jul;10(7):902-15. doi: 10.1128/EC.05095-11. Epub 2011 Jun 3. PubMed PMID: 21642510. 14. Raspelli E, Cassani C, Lucchini G, Fraschini R. Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization. Mol Biol Cell. 2011 Jul 1;22(13):2185-97. doi: 10.1091/mbc.E11-02-0127. Epub 2011 May 11. PubMed PMID: 21562220. 15. Finlayson, M.R. et al. (2011), Mol Biol Cell. Jul 7. [Epub ahead of print]. 16. Chatzimeletiou K, Morrison EE, Prapas N, Prapas Y, Handyside AH. Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy. Hum Reprod. 2005 Mar;20(3):672-82. Epub 2005 Feb 2. PubMed PMID: 15689349. 17. Chatzimeletiou K, Morrison EE, Panagiotidis Y, Vanderzwalmen P, Prapas N, Prapas Y, et al. Cytoskeletal analysis of human blastocysts by confocal laser scanning microscopy following vitrification. Hum Reprod. 2012 Jan;27(1):106-13. doi: 10.1093/humrep/der344. Epub 2011 Oct 25. PubMed PMID: 22028018. 18. Keeling JW, Miller RK. Indirect immunofluorescence for monitoring spindle assembly and disassembly in yeast. Methods Mol Biol. 2011;782:231-44. doi: 10.1007/978-1-61779-273-1_17. PubMed PMID: 21870296. Pictures: Immunohistochemistry: SM568P Alpha Tubulin antibody staining of Formalin- Fixed, Paraffin-Embedded Human Testis followed by biotinylated secondary antibody, alkaline phosphatase- streptavidin and chromogen.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20191119 3 / 4 SM568P: Monoclonal Antibody to alpha Tubulin / TUBA1B - Purified

Immunohistochemistry: SM568P Alpha Tubulin antibody staining of Formalin- Fixed, Paraffin-Embedded Human Nerve followed by biotinylated secondary antibody, alkaline phosphatase- streptavidin and chromogen.

Immunohistochemistry: SM568P Alpha Tubulin antibody staining of Formalin- Fixed, Paraffin-Embedded Human Brain, Cortex followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

SM568P Alpha Tubulin antibody staining of HeLa cells, green, nuclei are counterstained with DAPI.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20191119 4 / 4

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