SSM_2017 Basel 30/08/2017 Spread of antibiotic resistance markers in Lake Ceresio and tributary rivers. Monitoring results 2016
Federica Mauri Introduction and aims
Monitoring of Ceresio Observe if anthropic lake and tributary rivers activities could influence for the presence of the bacterial populations antibiotic resistance naturally present in water genes and bacteria environments
To gather more information about the role of aquatic ecosystem in spread of antibiotic resistance
2 Introduction
Sampling points
Rivers sampling points (jan-dec)
Rivers influenced by waste water treatment plant
Lake sampling points (feb, may, aug, nov)
Sampling points near potable water uptake plant
3 Results Total cells count: rivers Plate count 1.0E+07
1.0E+06
1.0E+05 Vedeggio CFU/mL 1.0E+04
1.0E+03
1.0E+02 Jan Feb Mar Apr May Jun Jul Aug Sep Oct Nov Dec Magliasina Flow cytometry 1.0E+07 Laveggio 1.0E+06
1.0E+05 Cells/ml Cassarate 1.0E+04
1.0E+03 Jan Feb Mar Apr May Jun Jul Aug Oct Nov Dec 5 Results Total cells count lake Plate count 1.0E+07 1.0E+06 1.0E+05 Gandria 1.0E+04 CFU/mL 1.0E+03 1.0E+02 1.0E+01 Melide 1.0E+00 Feb May Aug Nov Flow Cytometry Ponte Tresa 1.0E+07
1.0E+06
1.0E+05 Riva San Vitale
1.0E+04
1.0E+03 Cells/ml 1.0E+02 Lugano
1.0E+01
1.0E+00 Feb May Aug Nov 4 Results Antibiotic resistance genes: rivers
Rivers influenced by waste water treatment plant
Rivers NOT influenced by waste water treatment plant
FQLs: fluoroquinolones; Bla: β- lactams; Suls: sulphonamides; TETs:
tetracycline 7 Results Antibiotic resistance genes: lake
Absence of resistance genes
Presence of resistance genes
Sampling points Sample type Antibiotics Integrons ABR genes Feb May Aug Nov Gandria Integrated 0-20 m - Class I intI Melide Integrated 0-20 m - Class I intI FQLs - qnrS Ponte Tresa Integrated 0-20 m - Class I intI Bla - bla (TEM) Lugano 40 m Suls - sulII - Class I intI Riva San Vitale 33 m - Class I intI
FQLs: fluoroquinolones; Bla: β-lactams; Suls: sulphonamides
6 Results qPCR of ABR genes: rivers Genecopies/mL
16S sul II tetA qnrS bla (TEM) 9 Results qPCR of ABR genes: lake
16S
sulII
qnrS
8 Results Antibiotic resistant species
Bla % TETs % Suls % FQLs % Pseudomonas 18 17 24 13 Acinetobacter 19 24 18 25 % of most resistant Enterobacteriaceae 11 13 8 9 strains in rivers Aeromonas 3 3 5 9 Flavobacteriaceae 0.57 1 0.73 0.24 Comamonas 0.34 0.20 0.18 0.24
Bla % TETs % Suls % FQLs % Pseudomonas 13 9 31 3 Microbacterium 3 1.4 5 17 Stenotrophomonas 10 14 10 22 Brevundimonas 1.8 1.7 4 10 % of most resistant Acinetobacter 8 9 14 10 strains in lake Aeromonas 11 18 2.3 6 Enterobatteriacee 4 2 2 0 Sphingobium 2 0 0 0 Flavobacteriaceae 2 5 0 0
FQLs: fluoroquinolones; Bla: β-lactams; Suls: sulphonamides; TETs: tetracycline 10 Conclusions
ARGs were most sulII, qnrS, tetA present in rivers are the most influenced by abundant genes WWTP and in the lake near the coast .
Rivers and lakes are reservoir of Pseudomonas , Acinetobacter , Anthropic impact resistance genes Aeromonas , and resistance Enterobacteriaceae, bacteria Stenotrophomonas,
intI in all sampling points Mobilization of ARGs
11 Many Thanks to:
Prof. Dr Mauro Tonolla Laboratory of Applied Microbiology SUPSI Dr. Antonella Demarta Laboratory of Applied Microbiology SUPSI Dr. Andreas Bruder Institute of Earth Sciences SUPSI Dr. Giuseppe Ranieri Ufficio monitoraggio ambientale (SPAAS) Dr. Nicola Solcà Ufficio della gestione dei rischi ambientali e del suolo Dr. Gianluca Corno National Research Council of Italy (CNR)
Thank you for your attention! Results Antibiotic resistance genes: rivers
Antibiotics ABR genes jan feb mar apr may jun jul aug sep oct nov dec Antibiotics ABR genes jan feb mar apr may jun jul augsep oct nov dec bla (TEM) bla (TEM) bla (SHV) bla (SHV) β-lactams β-lactams bla ( CTX-M) bla ( CTX-M) bla (oxa 48) bla (oxa 48) qnrA qnrA Fluoroquinolones Fluoroquinolones qnrS qnrS tetA tetA Vedeggio Tetracycline tetM Cassarate Tetracycline tetM tetB tetB sulII sulII Sulphonamides Sulphonamides sulIII sulIII
Integrase intI Integrase intI
Antibiotics ABR genes jan feb mar apr may jun jul aug sep oct nov dec Antibiotics ABR genes jan feb mar apr may jun jul augsep oct nov dec bla (TEM) bla (TEM) bla (SHV) bla (SHV) β-lactams β-lactams bla ( CTX-M) bla ( CTX-M) bla (oxa 48) bla (oxa 48) qnrA qnrA Fluoroquinolones Fluoroquinolones qnrS qnrS tetA tetA Laveggio Tetracycline tetM Tetracycline tetM Magliasina tetB tetB sulII sulII Sulphonamides Sulphonamides sulIII sulIII
Integrase intI Integrase intI
Absence of resistance genes
Presence of resistance genes