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FOR IN VITRO RESEARCH USE ONLY Europe: [email protected] NOT FOR USE IN HUMANS OR ANIMALS China: [email protected] CREST Polyclonal An body Catalog number: 12439-1-AP Background Size: 39 μg/150 μl SS18-like 1(SS18L1) is a transcrip onal ac vator that is required for calcium- Source: Rabbit dependent dendri c growth and branching in cor cal neurons. It's also a nuclear Isotype: IgG protein interacts with CREB-binding protein and expressed in the developing brain. Synonyms: It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N- SS18L1; CREST, KIAA0693, terminal domain of SS18L1 is required for suppressing transac va on in the basal LP2261, SS18 like protein 1, state, while the C-terminal domain is required for calcium-induced transac va on. SS18L1, SYT homolog 1 It's part of the CREST-BRG1 complex, a mul protein complex that regulates promoter ac va on by orchestra ng a calcium-dependent release of a repressor complex and a recruitment of an ac vator complex. This complex also binds to the NR2B promoter, and ac vity-dependent induc on of NR2B expression involves a release of HDAC1 and recruitment of CREBBP
Applications Tested applica ons: ELISA, IHC, IP, WB Immunohistochemical of paraffin- Cited applica ons: CoIP, IF, IHC, WB embedded human breast cancer using 12439-1-AP(CREST an body) at Species specificity: Human,Mouse,Rat; other species not tested. dilu on of 1:50 (under 10x lens) Cited species: Fly, human, mouse Caculated CREST MW: 396aa,43 kDa Observed CREST MW: 55 kDa Posi ve WB detected in K-562 cells, COLO 320 cells, human heart ssue Posi ve IP detected in Mouse brain ssue Posi ve IHC detected in Human breast cancer ssue, human brain ssue Recommended dilu on: WB: 1:500-1:5000 IP: 1:500-1:5000 IP Result of an -CREST (IP:12439-1- IHC: 1:20-1:200 AP, 3ug; Detec on:12439-1-AP 1:1000) with mouse brain ssue lysate Applica on key: WB = Western blo ng, IHC = Immunohistochemistry, IF = Immunofluorescence, IP = Immunoprecipita on 6000ug. Immunogen information Immunogen: Ag3119 GenBank accession number: BC034494 Gene ID (NCBI): 26039 Full name: Synovial sarcoma transloca on gene on chromosome 18-like 1
K-562 cells were subjected to SDS Product information PAGE followed by western blot with Purifica on method: An gen affinity purifica on 12439-1-AP( CREST An body) at Storage: PBS with 0.1% sodium azide and 50% glycerol pH dilu on of 1:1000 7.3. Store at -20oC.