BioVision rev.06/17 For research use only Oxidase Antibody 1 2 3 170- CATALOG NO: A1116-30T 30 µg (Trial size) 125- A1116-100 100 µg 93-

ALTERNATIVE NAMES: Glycine oxidase, glycine oxygen (deaminating), 68-

GO 53- Western blot analysis of Glycine Oxidase CONCENTRATION: 0.5 mg/ml 41- using anti- Glycine Oxidase antibody:

Lane 1: r Glycine Oxidase (Cat. No. 7845) 2ng IMMUNOGEN: Glycine Oxidase H244K (Cat. No. 7845-1) 32- Lane 2: r Glycine Oxidase (Cat. No. 7845) 10ng 23- Lane 3: r Glycine Oxidase (Cat. No. 7845) 50ng INTERNAL ID: BV-Q43

170- MOLECULAR WEIGHT: 41 kDa 14- 170- 10- 125- HOST/ISOTYPE: Rabbit IgG 12593-

SPECIES REACTIVITY: Bacillus subtilis 1706893- 12568- PURIFICATION: Affinity purified rabbit IgG 93- 53- 68- FORM: Liquid 4153- RELATED PRODUCTS: 4153- FORMULATION: 0.5 mg/ml of antibody in PBS pH 7.2, 0.01 % BSA, 0.03 % 41- ProClin® and 50 % glycerol 32-  Glycine Oxidase H244K, Bacillus subtilis recombinant (Cat. No. 7845-250) 1 2 3 4 STORAGE CONDITIONS: Store at -20°C. Avoid repeated freeze/thaw cycles. kDa 3232-  Glycine Assay Kit (Fluorometric) (Cat. No. K589-100) 23- kDa 1 2 3 4 2323- DESCRIPTION: Glycine oxidase (GO) from Bacillus subtilis (EC 1.4.3.19) is a  Sarcosine Colorimetric/Fluorometric Assay Kit (Cat. No. K636-100) 170- 170- homotetrameric flavin-dependent oxidoreductase. Each GO  Cystathionine β Synthase, human recombinant (Cat. No. 7844-100) 125- 14- 125170- monomer is non-covalently bound to flavin adenine dinucleotide. 17014-- 93- 1014- 125- GO catalyzes oxidative deamination of various primary and  Cystathionine β-Synthase Antibody (Cat. No. A1112-100) 93- 68- 12510-- secondary amines (e.g. glycine, sarcosine, N-ethylglycine) and  Cytochrome Oxidase Activity Colorimetric Assay Kit (Cat. No. K287-100) 17010-- 6893- some D-amino acids (e.g. D -alanine, D -proline, D -valine) to the 53-Western 93- blot analysis of Glycine Oxidase Western using blotanti -analysis of CBS using anti-  Glucose Oxidase Activity Colorimetric/Fluorometric Assay Kit (Cat. No. K788- 125- 5368- CBS antibody: corresponding α-keto acids and hydrogen peroxide. Primarily, WesternGlycine Oxidase blot analysis antibody: of Glycine OxidaseWestern using blotanti -analysis of CBS using anti- 41- 68- 53- Lane 1: Hela cell lysat 60 ug glycine oxidase catalyzes the oxidation of glycine in the 100) 93- 41- CBS antibody: Glycine Oxidase antibody: Lane 2: CBS 2 ng biosynthesis of thiamine. The variant H244K shows a higher Lane53 1:- Glycine41 -Oxidase (Cat# 7845) 2ngLane 1: Hela cell lysat 60 ug  Activity (Total MAO/MAO-A/MAO-B) Fluorometric Assay Kit 68- Lane 2:3: CBS 210 ng ng substrate specificity ratio for glycine versus sarcosine and a 5-fold 32-Lane 12: Glycine Oxidase (Cat# 7845) 10ng2ng (Cat. No. K795-100) 41- 32- LaneLane4: 3: CBSCBS 5010 ng ng improved specific activity in comparison to the wild-type. 23-Lane 53 23:- Glycine Oxidase (Cat# 7845) 10ng 50ng 2332- Lane4: CBS 50 ng Lane 32 3:- Glycine Oxidase (Cat# 7845) 50ng APPLICATION: Western blot: 1-4 µg 41- 23-

FOR RESEARCH USE ONLY! Not to be used on humans. 14- 23- Western32- blot14 analysis- of HRV14 3C using anti- HRV14 Note: This information is only intended as a guide. The 3C antibody: optimal dilutions must be determined by the user. 14- 23-

Lane14 1- : HRV14 3C (Cat# 8012) 2ng

Lane 2: HRV14 3C (Cat# 8012) 10ng 10- Lane14 3- : HRV14 3C (Cat# 8012) 50ng

10-

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