Revision: 0 Effective Date: 9/4/2018 of isolation For Procedure Test 4. 3. 2. 1. Preparation 1. Precaution to required as supplemented and/or adjusted be may Formula 7. Final pH: Agar Brilliant Bismuth Ferrous Disodium Dextrose Beef Enzymatic Enzymatic Typical Formulation the and industrial applications for medium of isolation the most of detection routine for accepted is generally Agar p the increasing Sul on Bismuth Gram colonies. black characteristic forming medium, on the Sul Bismuth consumption. human for distribution before monitored with contaminated or improperly raw, undercooked, of consumption from result can Description to FDA/BAMformulation. conforms and humans in conditions other disease or of in diagnosis use the for intended not is Agar Sulfite Su Bismuth Use Intended Sheet Specification Technical

colonies. of inhibition the for potential and an increased microorganisms competing of selectivity may change medium storagethe of 3days after 2 days; than Sul Bismuth prepared store to recommended is not it However, samples. Sul Bismuth prepared Freshly dispensing. to suspension prior uniform a obtain to Mix thoroughly minute. one boil for and agitation frequent with Heat water. purified inof one liter the medium of 52 g Suspend toSDS Refer

E xtract G

S S

reen ul P ul D D 7 hosphate lf f

f f ate

igest of of igest of igest ite formula. Blair and of is a Wilson modification Agarite Agarite

± 0.2 at 25°C 25°C at 0.2 ±

S. typhi typhi S. f typhi Salmonella

I ite Agar. The inhibitory action of Bismuth Sul Bismuth of action inhibitory ite The Agar. ossibility of recovering pathogens that may be present in small numbers. BismuthSul numbers. small in present bemay that pathogens recovering of ossibility

ndicator

Salmonella

A C

is used for the selective isolation of of isolation the selective for is used

nimal nimal asein and other and

T BismuthSul issue . U. S. federal guidelines require various poultry products to be routinely be routinely to products poultry various require guidelines S. federal . U. and other other and Salmonella Salmonella f ite Agar is very selective, therefore suitable for heavily contaminated heavily contaminated for suitable therefore selective, very is Agar ite

0.025 g/L g/L 0.025 20 8 0.3 g/L 4 5 5 5 5 .0 .0 .0 .0 .0 .0 .0 laboratory laboratory Salmonella Salmonella

g/L g/L g/L g/L g/L g/L g/L g/L g/L spp. from food from spp. f

ite Agarite

environment. spp. consult appropriate references. appropriate consult spp.

Salmonella Salmonella Salmonella Salmonella - . Bismuth Sul . Bismuth

positive bacteria and coliforms areinhibited coliforms and positive bacteria f ( ite Agar permits the use of a large inoculum, inoculum, a large of the use permits Agar ite

NCM0086 meet performance specifications. performance meet

to a green color with a reduction in in a with reduction color a green to

The typhoid typhoid The spp. Bismuth Sul Bismuth spp. spp.

f In a laboratory setting. Bismuth Bismuth setting. a laboratory In ite Agar is a standard methods methods is a standard Agar ite ) f

ite Agar at 4°C for longer longer for at4°C Agar ite organism grows luxuriantly organism processed foods foods processed f ite Agar is used for used for is Agar ite

Salmonella

f ite

Revision: 0 Effective Date: 9/4/2018 closed. tightly container by keeping and light moisture from Protect storage temperature. the same at environment humidity low in a container 2 media: culture Dehydrated Storage 4. 3. 2. 1. Procedures the of Limitations container. in its intact medium to applies Expiry color. the original from has changed appearance or if flowing, free date toexpiration Refer Expiration appearance. a crater exhibit and centers depressed with colonies raised green, to brown produce this medium than other spp. Salmonella of strains Other the the colony. sizeof times be several zone may This sheen. a metallic or without Typical Results testing. qualitycontrol used thatfor beshould minimum the listed are organisms The incubation. Response: Cultural Expected andgreen light medium, Appearance: Prepared Appearance: Dehydrated Specifications Control Quality Sheet Specification Technical

Salmonella Salmonella Enterococcus faecalis Enterococcus Do not expose Bismuth Sulfite Agar tosunlight. Sulfite Agar Bismuth expose Do not properties. selectivity destroy may period along for Heating medium medium. autoclave Do not strains. allof typhoid growth to allow hours 48 total of a for be incubated should all plates however, However, S. typhi Atypical Salmonella Shigella Escherichia Escherichia coli Escherichia S

.

typhi typhi

produce black to green colonies with little or no darkening of surrounding medium. medium. surrounding of or nodarkening littlewith colonies to green black produce

and and

S. typhi Microorganism typhimurium S. arizonae S. arizonae S surface colonies are colonies surface S. arizonae arizonae S. . flexneri flexneri appears light appears

ATCC® 25922 ATCC® ATCC® 12022 ATCC® ATCC® 8739 ATCC®

stamped on the container. The dehydrated medium should be discarded if not not if discarded be should medium dehydrated The on the container. stamped

ish Prepared medium is opaque and may have a few dark particles suspended in particles suspended dark a few have and may is opaque medium Prepared ATCC® 13076 ATCC®

ATCC® 29212 ATCC®

is usually inhibited. usually is and and ATCC® 14028 ATCC® - Powder is homogeneous, free flowing, and beige to green to beige and flowing, free homogeneous, is Powder yellowto - are the only enteric organisms to exhibit typical brown zones on the medium. the medium. onzones brown typical to exhibit organisms enteric only the are 30

S. sonnei sonnei S. o

Cultural response on Bismuth Sulfite Agar at 35 ± 2°C after 40 40 2°C± after at 35 Agar Sulfite Bismuth on response Cultural

C away from direct sunlight. direct sunlight. from away C

green and may be misinterpreted maybe and green

olive light/medium light/medium

are inhibited. inhibited. are -

green to green Inoculum Inoculum

Approx. Approx. 100 100 Typical Typical ≥1000 ≥1000 ≥1000 ≥1000 (CFU) - - 300 300 green black, surrounded by black orbrown black by surrounded black,

S. typhi S. typhi

S. flexneri S. flexneri ish Completely inhibited Completely completely inhibited completely inhibited completely completely inhibited completely Fair to excellent Fair to Fair to excellent Fair to beige. beige.

Once opened and recapped, place place recapped, and opened Once colonies usually develop within 24 hou within 24 develop usually colonies Partially to Partially to Partially Partially to Partially Growth and and

as non as S. sonnei sonnei S.

Expected Results Expected - Salmonella sp Salmonella

strains that do grow on on do grow that strains Green Olive Pale green to olive to Pale green olive to Pale green Green to brown colonies brown toGreen

ish -

- black to black colonies colonies to black black ± metallic sheen ± metallic sheen ± metallic to olive to - black zone with zonewith black beige. beige. p Reactions . colonies colonies

Shigella - -- -

green colonies colonies green 48 hours hours 48

- like like

rs; rs;

- -

green green green

Revision: 0 Effective Date: 9/4/2018 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. References Sheet Specification Technical

Cook, G.T. G.T. Cook, MD. Baltimore, & Wilkins, Vol. 1. Williams J.F. MacFaddin, VA. Arlington, P. (ed.). Cunniff, manualBAM/default.htm www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical MD. Rockville, Convention, Pharmacopeial Pharmacopeia States United microbiological (eds.). Splittstoesser D.F. and C., Vanderzant, D.C. , Washington, (ed.). H.D. Isenberg, of isolation Blair. E.and M. J., W. Wilson, of Blair. E.and M. J., W. Wilson, 29 the typhoid for medium selective and enrichment Blair. E.and M. J., W. Wilson, :310. :310. B. typhosus

B. typhosus typhosus B. 1952. J. Path. B Path. J. 1952.

and examination of foods, foods, of examination Official method Official 1985. Media for isolation for Media 1985. B. proteus B. 1992. Clinical microbiology procedures handbook, vol. 1. American Society for Society for 1. American vol. handbook, procedures microbiology Clinical 1992. and and .

act. act. B. proteus . J. Hyg. Hyg. . J. . The United States States United . The 1931. Further experience of the bismuth sulphite media in the media sulphite the bismuth of experience Further 1931. the isolation for medium iron sulphite bismuth a glucose of Use 1927. an affording sulphite and bismuth sodium of A combination 1926. 64:

s of analysis of AOAC International, International, AOAC of analysis of s 559. 559. 4th . J. Hyg. Hyg. . J. 26

:374 ed. Ameican Public Health Association, Washington, D.C. Washington, Association, Public Health Ameican ed.

-

cultivation -

391. 391. 31 - 2015 Bacteriol. Pathol. J. bacteria. of groups paratyphoid :138 P

harmacopeia, harmacopeia, - . Compendium of methods for the for of methods . Compendium - identification

161. 161.

41st 41st - maintenance of medical bacteria, bacteria, medical of maintenance 20 ed. The United States States United The ed. th ed. AOAC International, International, AOAC ed. th