Anti-HIP2 Antibody (APC) Product Number: AC21-0298-03

Overview Host Species: Goat Clonality: Polyclonal Purity: Affinity purified using the immunizing peptide. The affinity purified antibody is then conjugated to allophycocyanin (also known as APC) which is a fluorescent protein. Conjugate: APC Immunogen Type: HIP2 antibody (APC) was raised against a synthetic peptide of HIP2. The HIP2 peptide immunogen had the following sequence: C-SWDVETATELLLSN Physical Characteristics Amount: 0.05 mg Concentration: 0.5 mg/ml Volume: 0.1 ml Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Storage: APC conjugated antibody can be stored at 4?C for up to 18 months. For longer storage the conjugate can be stored at -20?C after adding 50% glycerol. APC conjugated antibodies should always be stored in the dark. Specificity Predicted Species Reactivity: Human, Mouse, Dog, Pig, Cow Target Information Accession Number(s): NP_005330.1, NP_001104582.1, NP_001104583.1 Gene ID Number(s): 3093 (human) Alternative Names: UBE2K; HIP2; huntingtin interacting protein 2; LIG; HYPG; ubiquitin-protein ligase; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); DKFZp564C1216; DKFZp686J24237; E2-25K Application Details Blocking Peptide: HIP2 peptide, catalog number AC21-0298-P, is the immunizing peptide for this product and can be used as a negative control to block antibody (APC) binding in a competition or adsorption assay. Tested Applications: ELISA, WB, IHC ELISA: Detection limit dilution 1:2000. Western Blot: Recommended starting concentration for western blot is 1-3µg/ml. Important Comments: Anti-HIP2 antibody (APC) is expected to recognize isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).

Page 1/1 All products are for research purposes only, not for diagnostic or therapeutic use. Abcore . 405 Maple Street, Suite A106 . Ramona, CA 92065 . T 888-358-1364 . F 888-884-9241 . www.abcore-inc.com