Ann Rheum Dis: first published as 10.1136/ard.42.Suppl_1.114 on 1 January 1983. Downloaded from

Suppl p 114 Annals ofthe Rheumatic Diseases

4 Kozin F, Ginsberg M H, Skosey J L. of human complement (C5) induced by simple method for measuring chemotaxis Polymorphonuclear leukocyte responses monosodium urate crystals: C5 conver- and spontaneous migration of human to monosodium urate crystals: modifica- tase assembly on the crystal surface. Clin polymorphonuclear leukocytes and tion by adsorbed serum proteins. J Immunol Immunopathol 1982; 24: monocytes. J Irnmunol 1975: 115: Rheumatol 1979; 6: 519-26. 239-50. 1650-6. 5 Russell I J, Mansen C, Kolb L M, Kolb 6 Nelson R D, Quie P G, Simmons R L. W P. Activation of the fifth component Chemotaxis under agarose: a new and

Inflammatory effect of phosphate

P. NETTER, J. L. DELONGEAS, G. FAURE, P. BOZ, D. BURNEL, J. L. FOLIGUET, M. F. KESSLER, R. J. ROYER, A. GAUCHER

Front the Travail de la Clinique Rhumatologique, des Laboratoires de Pharmiacologie, d 'A atooi ie patlhologique, de Chuinlie et d'Histologie, et du Service de Nephrologie, Faculte de Medecine de Nancs, Unilversiu' de Nan(c! 1, 5450() Vaindoeuvre les Nancv, France

Microcrystalline-induced arthritis is phosphate using two different animal wavelength dispersive microprobe known to occur in patients undergoing models: (a) paw oedema after analysis, aluminium associated w ith haemodialysis for chronic renal subcutaneous injection in the rear foot phosphate w\as tound in cellular insufficiency. The following pad of Sprague Dawley rats and (b) elements. These features are similar to microcrystals have been implicated so experimental arthritis after those observed in the patient in our far in these inflammatory phenomena: intra-articular injection in rabbits. preliminary study and those described by copyright. apatite, pyrophosphate Tribasic aluminium phosphate was in cerebral tissue from patients with dihydrate (CPPD), and compared to calcium hydrogen aluminium intoxication. monourate. phosphate dihydrate previously found The inflammatorv effect In preliminary study,' 2 using during destructive chondrocalcinosis demonstrated in this study suggests scanning electron microscopy (SEM) in humans4; natural diamond powder that in addition to apatite. calcium and wavelength dispersive and lambda carrageenan were chosen pyrophosphate dihydrate and sodium microanalysis (WDM), we found as negative and positive controls. moniourate. aluminiulmi compounds aluminium deposits associated with In the first model, inflammation could play a part in clinical phosphate in synovial tissue from a induced by aluminium phosphate maniifestations observed in patients patient undergoing haemodialysis reached its maximum as soon as 15-3t) with chronic renal insufficiencv because of chronic renal insufficiency, min after injection but lasted only 24 undergoing haemodialysis and http://ard.bmj.com/ who had been taking aluminium hours. The response to calcium receiving aluminium gels. hydroxide for five years. Aluminium hydrogen phosphate dihydrate was concentrations measured with a only minor whereas carregeenan- References sensitive and reproducible pulse induced inflammation appeared after polarography method were raised in two hours but was far greater and 1 Netter P, Bumel D, Hutin M F, Kessler the synovial fluid (87 Ag/l), synovial lasted mored than 24 hours. M F. Faure G. Aluminium in joint tissues tissue (3 7 and 2-2 wet weight) The second experimental of patient taking . gg/g Lancet 1981; i: 1056-7. was and in the cartilage (22 4g/g wet proceeding used to evaluate the 2 Netter P. L'aluminium dans l'os, le on September 27, 2021 by guest. Protected weight) compared with controls: histological consequences of the cartilage, la membrane synoviale et le synovial fluid: 10-8 + 5 .g/l (n = 6), injection of these compounds on liquide synovial. University of Nancy, synovial tissue: 0 8 0 2 g/g (n = 4), synovial tissue and cartilage. Rabbits Nancy, 1980. Thesis. cartilage: 8-31 + 2 0 ,ug/g (n = 4). were injected in the knee joints every 3 Royer R J, Delongeas J L, Netter P, et al. Transmission electron microscopy day for three days. Aluminium Inflammatory effect of aluminium on rat (TEM) revealed electron dense phosphate can induce, like paws. Pathol Biol (Paris) 1982; 30: 211-5. material of microfibrillar appearance carrageenan, inflammatory lesions of 4 Faure G, Netter P, Malaman B. in lysosomes of macrophagic synovial the synovial tissue and erosive damage Steinmetz J. Monocrystalline calcium cells. of the articular cartilage. With TEM, hydrogen phosphate dihydrate in In a second part we developed lysosomal inclusions of phagocytosed destructive arthropathies of experimental models to appreciate the material with microfilaments were chondrocalcinosis. Lancet 1977; ii: prophlogistic properties of aluminium seen. Through SEM coupled with a 142-3.