How to Upgrade from Vector NTI® to

benchling.com Modern Vector NTI® and Other Legacy Molecular Biology Tools deserves modern software Low throughput design Individual contributor In the past decade, molecular biology has evolved tremendously, but sequence Desktop-based design software has stagnated. Most sequence design tools are point solutions Manual design and analysis that weren’t built for modern scientific workflows or cross-team collaboration. Standalone tools While such tools might seem to “check the box” for certain functions, they ultimately can’t meet the full needs of complex R&D workflows, since they don’t meaningfully integrate with broader informatics infrastructures. As a result, Benchling Molecular Biology

R&D organizations struggle to tie downstream assay data back to sequences High throughput design and can’t fully leverage the data they’re producing. This regularly slows down Large collaborative teams research and and introduces risks into process development and scale up. Web-based Automated design and analysis Within today’s life science R&D organizations, molecular biology is high- Natively unified with the Benchling throughput, collaborative, and organizationally complex. To meet these needs, Life Sciences R&D Cloud companies need much more than a desktop-based point solution. They need cloud-based software unified with a broader informatics platform, so they can transfer context across teams and trace from sequences down to the results of individuals candidates.

With Vector NTI® set to be officially discontinued by Thermo Fisher, now is the ideal time for life science R&D organizations to digitally transform their molecular biology with a modern, cloud-based platform.

benchling.com 2 WHY BENCHLING 1 Benchling is built for modern science

Best in class high Features for the latest A single comprehensive throughput capabilities scientific workflows suite of tools

• Batch cloning, alignment, and • From CRISPR, to , • Unify CRISPR, sequence translation tools were designed to cloning, Benchling design, and more with 10+ for modern, high-throughput Molecular Biology is built to tools on one intuitive interface. workflows. power the latest techniques. • Translate directly from DNA • Bulk CRISPR guide design with • Share a gRNA repository with sequences to amino acid on-/off-target scoring is the your organization, access more sequences, automatically industry-leading tool for high- than 100 genomes, and create compute biochemical throughput CRISPR workflows. assemblies with cutting-edge properties for any sequence, cloning techniques. and perform multi-sequence alignments all in the same cloud-based tool.

benchling.com 3 WHY BENCHLING 2 Benchling is purpose-built for collaboration

Web-based, simple, and Seamless handoffs with full Access controls and role-based intuitive user interface experimental context permissions

• When replacing legacy systems • Use shareable links and • Grant Author-Only, Read, such as Vector NTI®, Benchling annotation libraries to Edit, and Admin permissions increases user adoption by standardize analysis across for particular sequences to over 90%. your teams. particular users.

• Benchling Molecular Biology • Track all sequence edits • Share specific sequences with was designed to be as usable as through a complete version external collaborators to limit modern consumer software. history. IP exposure.

• Trace from the assay data produced by physical samples to their upstream sequences.

benchling.com 4 WHY BENCHLING 3 Benchling is a unified data management platform, not a point solution

Native unification Track complex Cut down manual work Open Developer with other Benchling relationships with automation Platform for integration applications • Model complex • When registering a • Through modern • Benchling Molecular relationships between construct, Benchling REST APIs, easily Biology enables sequences and other automatically integrate Benchling workflows that biological entities. parses out, registers, with your existing IT standalone apps simply and interlinks the infrastructure. cannot support. • See the downstream construct's components. samples and results • Auto-sync with • Design and register that any sequence has • Automatically compute instruments, software, sequences in the led to. For example, the biochemical and databases. same interface. For see the binding affinity properties of constructs example, in Requests of all antibody lots based on parts • Automatically backup and Workflows, specify with a given light chain detection. all entities and related registered sequences to variable fragment. analysis. be used in fulfillment. In Notebook, drag-and- drop sequences to link to them in the middle of Notebook entries.

benchling.com 5 Professional services and training make for an Workflow Discovery Migration and and Configuration Integration effortless migration and Your dedicated Benchling Benchling will coordinate Customer Success team all configuration, data ongoing success takes the time to understand import, and automation your requirements, your integration activities in close research workflows, and your communication with you Our team is made of experienced biotech consultants, organizational structure. to ensure that the platform Silicon Valley software engineers, and molecular and cellular Based on these needs and on fits seamlessly into your industry best practices, we existing research and IT biology PhDs. We have published industry standards for life then design ideal workflows infrastructure. science R&D data models and have 100% customer retention for you and map your science and processes onto the across our hundreds of Benchling Enterprise clients. Benchling platform.

Go-Live Ongoing Success The same Customer Success Following the initial roll-out, representatives that craft your your Customer Success team Benchling instance will be the will continue to engage with ones to train your scientists you to ensure long-term on it. We offer comprehensive success, resolve any issues go-live training to get all that arise, and develop new stakeholders up and running. Benchling Molecular Biology Designated “super users” features. In addition, live are given additional training in-app support chat gives so that they can serve as an your scientists a direct line ongoing resource to your team. to Benchling Customer Success 24/7.

benchling.com 6 Case Study By migrating them off of Vector NTI®, Benchling cut down busywork at a Benchling has a successful top-10 pharma by 96% track record of transitioning customers from Before Vector NTI® • Customer had 100s of users with significant experience using Vector NTI®.

• Some workflows took up to 80 hours due to thelack of molecular biology automation.

• Experimental results were not linked with sequence data making it difficult to apply predictive machine learning.

• Clunky and dated software hadpoor uptake among users.

The Transition Process

1. Workflow Discovery and Configuration • Requirements gathering • Organization structure mapping • Understanding research workflows • Designing ideal workflows and industry best practices • Transferring all existing molecular biology data (including 1000s of legacy Vector NTI® files) • Project management

benchling.com 7 The Transition Process (continued) Case Study 2. Migration and Integration • Importing of data into Benchling • Integrating with Registry and other custom applications • Integrating with automation for request submission steps • Configuring Benchling platform • Versioning and audit log support

3. Go-Live and Ongoing Success ?

• Power user training and roll-out SL • Phased roll-out to users across multiple sites • Go-live training for all users • Post-roll out support • Regular updates and feature enhancements • Extensive feedback and feature request system • Robust roadmap of new functionality • Ongoing training

After

• Integrated Molecular Biology application with Benchling Registry and other internal systems - resulting in a more complete system of record.

• Synced Benchling platform with custom automation to handle request submission tasks freeing up scientists from redundant manual work (96% time savings).

• Flexible data models, sequence-level intelligence, and robust developer platform now power machine learning applications.

• Support for modern scientific workflows fostered higher uptake among scientists right from the start.

benchling.com 8 Benchling meets or exceeds Vector NTI® functionality in all core areas.

Functionality Benchling Vector NTI®

• Use from any computer anywhere Cloud-based • Wide OS support Platform • Import data from public databases

• Link sequences directly to notebook entries Native • Flexibly record results and properties of a variety of samples in a single software Integration with • Track lineage of biological entities with parent-child relationships Other Apps • Manage inventory locations of DNA and AA constructs

• Auto-detect and fill parts based on sequence-level intelligence Bio-intelligent • Smart compute biochemical properties of sequences Analysis • Import, search, and reuse oligos

• Share links and annotation libraries to standardize analysis across teams • Set-up role-based permissions for clear accountability Collaboration • Manage information sharing with advanced access controls • Share construct designs and parts with downstream teams

• Automate laborious and time consuming analysis steps Automation • Auto sync with instruments, software, and databases • Automatically backup all entities and related analysis

• Leverage professional services for effortless migration Support • Keep up-to-date with the science with regular feature updates

• Bulk cloning / assembly High- • Bulk auto-annotations throughput • Bulk auto-alignments Tools • Bulk primer attachment • Bulk translations

9 Functionality Benchling Vector NTI®

• Guide RNA design • On-target / off-target scoring CRISPR • HR templates • assembly

• Restriction-based cloning wizard Construct Design • Gibson assembly • Golden gate assembly

• Primer creation wizard • Pre-existing primer library import Primer • Gibson primer design Design • Golden gate primer design • Secondary structure checks

• Enzyme search • Virtual digests Cloning • Enzyme cut sites • Ladders library • Enzyme lists

• Plasmid map Sequence • ORF customization Visualization • Annotations • Sequence search

Sequence •Alignment to templates Alignment • Consensus alignments

• AA alignments AA / • Translations Analysis • Biochemical properties computation Codon Optimization Coming in Q2, 2019 RNA Design and Analysis 2020 Roadmap Other Assembly Technologies 2020 Roadmap Circuit Visualization 10 Benchling has modernized molecular biology at hundreds of companies.

Request a demo to learn more about how to upgrade from Vector NTI® to Benchling www.benchling.com/request-demo/

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