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Reader Service No. 233 GENES

DEVELOPMENT

VOLUME 8 NUMBER 19 PAGES 2257-2388 OCTOBER 1, 1994

EDITORIAL BOARD J. Adams (Melbourne, Australia) N. Jones (London, UK) Editors J. Beckwith (Boston, USA) J. Kadonaga (La Jolla, USA) T. Grodzicker (Cold Spring Harbor) T. Cech (Boulder, USA) R. Lehmann (Cambridge, USA) N. Hastie (Edinburgh) P. Chambon (Strasbourg, France) M. Levine (San Diego, USA) N.-H. Chua {New York, USA) D. Livingston (Boston, USA) Managing Editor E. Coen (Norwich, UK) R. Losick (Cambridge, USA) J. Cuddihy (Cold Spring Harbor) J. Coffin (Boston, USA) J. Manley (New York, USA) S. Courtneidge (Heidelberg, FRG) W. McGinnis (New Haven, USA) E. De Robertis (Los Angeles, USA) S. McKnight (South San Francisco, USA) R. Evans (La Jolla, USA) A. McMahon (Cambridge, USA) G. Fink (Cambridge, USA) P. Nurse (London, UK) P. Goodfellow (Cambridge, UK) C. Nfisslein-Volhard (Tubingen, FRG) S. Gottesman (Bethesda, USA) R. Palmiter (Seattle, USA) T. Graf (Heidelberg, FRG) U Parada (Dallas, USA) C. Gross (San Francisco, USA) C. Prives (New York, USA) R. Grosschedl (San Francisco, USA) G. Rubin (Berkeley, USA) F. Grosveld (Rotterdam, The Netherlands) U. Schibler (Geneva, Switzerland) M. Groudine (Seattle, USA} D. Solter (Freiburg, FRG) L. Guarente (Cambridge, USA) P. Soriano (Seattle, USA) R. Harland (Berkeley, USA) J. Steitz (New Haven, USA) E. Harlow (Charlestown, USA) T. Taniguchi (Osaka, Japan) W. Herr (Cold Spring Harbor, USA) S. Tilghman (Princeton, USA} J. Hodgkin (Cambridge, UK) R. Tjian (Berkeley, USA) R. Horvitz (Cambridge, USA) E. Wagner (Vienna, Austria) P. Ingham (London, UK) V. Walbot (Stanford, USA) T. Jessell (New York, USA) M. Wigler (Cold Spring Harbor, USA)

EDITORIAL OFFICES Editorial~Production United States United Kingdom N. Dumser, Technical Editor Cold Spring Harbor Laboratory Press MRC Human Genetics Unit V. Nicolette, Production Editor Box 100, 1 Bungtown Road Western General Hospital L. Olsewski, Editorial Secretary Cold Spring Harbor, New York 11724-2203 Crewe Road Phone 516-367-8492 Edinburgh EH4 2XU, UK FAX 516-367-8532 Phone 031-332-8676 FAX 031-332-7359

GENES & DEVELOPMENT(ISSN 0890-9369 is published semimonthly for $450 Veronica van Heyningen, MRC Human Genetics Unit, Western General (institutional), $115 (individual making personal paymentl, $159 (Genet- Hospital, Crewe Road, Edinburgh EH4 2XU, UK. ical Society of Great Britain members) by Cold Spring Harbor Laboratory Claims for missing issues must be received within 4 months of issue Press, Bungtown Road, Cold Spring Harbor, New York 11724, in associ- date. ation with the Genetical Society of Great Britain. Second-classpostage is Change of address Please enclose recent mailing label with address paid at Cold Spring Harbor and additional mailing offices. POSTMAS- change; allow 4 weeks. TER: Send address changes to Cold Spring Harbor Laboratory Press, POB 100, 1 Bungtown Rd., Cold Spring Harbor, New York 11724-2203. Advertising To advertise in Genes & Development, contact Nancy Subscription Price Orders may be sent to Cold Spring Harbor Labora- Kuhle, Advertising Manager, Cold Spring Harbor Laboratory Press, Cold tory Press, Fulfillment Department, 10 Skyline Drive, Plainview, NY Spring Harbor, New York 11724-2203; telephone 516-367-8351. 11803-9729. Telephone: Continental US except NY State, 1-800-843- Photo Copy Authorization to photocopy items for internal or personal 4388~ all other locations, 516-349-1930/1931/1932. FAX 516-349-1946. use, or the internal or personal use of specific clients, is granted by Cold Volume 8, 1994, $450, U.S. institutional; $540, R.O.W. institutional. Spring Harbor Laboratory Press for libraries and other users registered Personal subscription rate: $115, U.S.; $205, R.O.W. Price includes sur- with the Copyright Clearance Center (CCC) Transactional Reporting face postage for U.S. and airlift for R.O.W. Genetical Society members, Service, provided that the base fee of $5.00 per copy is paid directly to $159.00. All subscriptions are entered for the calendar year and must be CCC, 21 Congress St., Salem, MA 01970 (0890-9369/94 $5.00 + 0.}. This prepaid. Personal subscriptions must be prepaid by personal check, consent does not extend to other kinds of copying, such as copying for credit card, or money order. All checks must be for US dollars and drawn general distribution for advertising or promotional purposes, for creating on a US bank. Genetical Society members may also subscribe by check, new collective works, or for resale. payable to the Genetical Society, for s (includes airlift). Send to: Dr. Copyright 1994 by Cold Spring Harbor Laboratory Press GENES & DEVELOPMENT October 1, 1994 Contents

Research papers Evidence that a complex of SIR interacts with the silencer and telomere-binding 2257 RAP1 Paolo Moretti, Katie Freeman, Lavanya Coodly, and David Shore

Diploidy of Drosophila imaginal cells is maintained by a transcriptional repressor encoded 2270 by escargot Naoyuki Fuse, Susumu Hirose, and Shigeo Hayashi

Spl sites in the mouse aprt gene promoter are required to prevent methylation of the 2282 CpG island Donald Macleod, Jillian Charlton, John Mullins, and Adrian P. Bird

Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and 2293 embryonic lethality in mice Zhi Chen, Glenn A. Friedrich, and Philippe Soriano

The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis 2302 Holly A. Ingraham, Deepak S. Lala, Yayoi Ikeda, Xunrong Luo, Wen-Hui Shen, Mark W. Nachtigal, Rula Abbud, John H. Nilson, and Keith L. Parker

Drosophila TFIIA directs cooperative DNA binding with the TBP and mediates 2313 transcriptional activation Kyoko Yokomori, Martin P. Zeidler, Jin-Long Chen, C. Peter Verrijzer, Marek Mlodzik, and Robert Tjian

Molecular cloning of the small ([3) subunit of human TFIIA reveals functions critical for 2324 activated transcription Josef Ozer, Paul A. Moore, Arthur H. Bolden, Arianna Lee, Craig A. Rosen, and Paul M. Lieberman

Reconstitution of human TFIIA activity from recombinant polypeptides: a role in 2336 TFIID-mediated transcription Xiaoqing Sun, Dongmin Ma, Michael Sheldon, Kam Yeung, and Danny Reinberg

RRN6 and RRN7 encode subunits of a multiprotein complex essential for the initiation of 2349 rDNA transcription by RNA polymerase I in Saccharomyces cerevisiae Daniel A. Keys, Loan Vu, Joan S. Steffan, Jonathan A. Dodd, Robert T. Yamamoto, Yasuhisa Nogi, and Masayasu Nomura

Evidence that the cis preference of the Tn5 transposase is caused by 2363 nonproductive multimerization Michael D. Weinreich, Audrey Gasch, and William S. Reznikoff

A genetic link between light response and multiceUular development in the bacterium 2375 Myxococcus xanthus Francisco J. Nicolas, Rosa M -~ Ruiz-V~zquez, and Francisco J. Murillo

Cover Ectopic esgargot expression inhibits growth of Drosophila salivary gland. (Background) DAPI staining of salivary gland. (Inset) Gal4: dizf salivary glands and eye-antennal discs. (For details, see Fuse et al., p. 2270.)