Catalog # Aliquot Size

P50-911B-05 3 x 5 nmol P50-911B-20 3 x 20 nmol P50-911B-50 3 x 50 nmol

PTPRC (CD45) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # P50-911B

Lot # Z2085-28

Specificity Formulation PTPRC (CD45) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human PTPRC (CD45) expression. shipped at room temperature.

Product Description Reconstitution Protocol PTPRC (CD45) siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human PTPRC collect lyophilized siRNA at the bottom of the tube. (CD45) mRNA expression. Each siRNA is 19-25 bases in Resuspend the siRNA in 50 µl of DEPC-treated water length. The accession number is NM_001111316. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot B220; Cd45; CD45R; loc; Ly-5; Lyt-4; T200 1x stock solutions for storage.

Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or PTPRA (LRP), Active P48-21G below -20oC. After resuspension, siRNA stock solutions ≥2 PTPRC (CD45), Active P50-21H µM can undergo up to 50 freeze-thaw cycles without PTPRC (CD45), Active P50-21BG significant degradation. For long-term storage, it is PTPRE, Active P52-21G recommended that the siRNA is stored at -70oC. For most PTPRF (LAR), Active P53-21G favorable performance, avoid repeated handling and PTPRS(PTP-sigma), Active P64-21G multiple freeze/thaw cycles. PTPRM (PTP-mu) P58-35G

Scientific Background PTPRC (CD45) contains an extracellular domain, a single transmembrane segment and two tandem PTPRC (CD45) siRNA Set I siRNA duplexes targeted against three exon regions intracytoplasmic catalytic domains and thus belongs to receptor type PTP (1). This gene is specifically expressed in Catalog # P50-911B hematopoietic cells (2). PTPRC has been shown to be an Lot # Z2085-28 essential regulator of T- and B-cell antigen receptor Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol signaling. It functions through either direct interaction with Format Lyophilized powder components of the antigen receptor complexes, or by Stability 1yr at -70oC from date of shipment activating various Src family kinases required for the Storage & Shipping The lyophilized powder is stable for at antigen receptor signaling. least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at References or below -20oC. After resuspension, 1. Ralph, S. J. et al: Structural variants of human T200 siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles glycoprotein (leukocyte-common antigen). EMBO J. without significant degradation. For 6: 1251-1257, 1987. long-term storage, it is recommended 2. Fischer, E. H. et al: Protein tyrosine phosphatases: a that the siRNA is stored at -70oC. For most favorable performance, avoid diverse family of intracellular and transmembrane repeated handling and multiple enzymes. Science 253: 401-406, 1991. freeze/thaw cycles.

To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com

FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.