MYLK Antibody Cat. No.: 23-364

MYLK Antibody

Immunohistochemistry of paraffin-embedded human Immunohistochemistry of paraffin-embedded rat kidney using MYLK antibody stomach using MYLK antibody (23-364) at dilution of 1:100 (40x lens). (23-364) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using MYLK antibody (23-364) at dilution of 1:100 (40x lens).

Specifications

September 27, 2021 1 https://www.prosci-inc.com/mylk-antibody-23-364.html HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids IMMUNOGEN: 1765-1914 of human MYLK (NP_444253.3).

TESTED APPLICATIONS: IHC, WB

WB: ,1:500 - 1:2000 APPLICATIONS: IHC: ,1:50 - 1:100

POSITIVE CONTROL: 1) HeLa

2) HepG2

3) HT-1080

PREDICTED MOLECULAR Observed: 170kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: MYLK

myosin, light polypeptide kinase, myosin light chain kinase, MSTP083, MLCK210, ALTERNATE NAMES: MLCK108, MLCK, KRP, FLJ12216, DKFZp686I10125, myosin, light chain kinase, MYLK, MLCK1, MYLK1

GENE ID: 4638

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 27, 2021 2 https://www.prosci-inc.com/mylk-antibody-23-364.html This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light BACKGROUND: chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 27, 2021 3 https://www.prosci-inc.com/mylk-antibody-23-364.html