Camkk2 Antibody (R30570)

CaMKK2 Antibody (R30570)

Catalog No. Formulation Size

R30570 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request

Availability 1-3 business days

Species Reactivity Human, Mouse, Rat

Format Antigen affinity purified

Clonality Polyclonal (rabbit origin)

Isotype Rabbit IgG

Purity Antigen affinity

Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

UniProt Q96RR4

Applications Western blot : 0.5-1ug/ml IHC (FFPE) : 0.5-1ug/ml Immunocytochemistry : 0.5-1ug/ml

Limitations This CaMKK2 antibody is available for research use only.

Western blot testing of CaMKK2 antibody and Lane 1: rat brain; 2: mouse brain IHC-P: CaMKK2 antibody testing of rat brain tissue

Description Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CaMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.

Application Notes The stated application concentrations are suggested starting amounts. Titration of the CaMKK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen An amino acid sequence from the middle region of CaMKK2 (KLAYNENDNTYYAMK) was used as the immunogen for this CaMKK2 antibody (100% homologous in human, mouse and rat).

Storage After reconstitution, the CaMKK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.