B-7000 Mons 23, place du parc 0032 (0) 65 37 38 03 * Laboratory Research University - Impact of pollen - - - Modifica on of pollen - Pollen profiles: R [email protected] - - - collected In M
generalist highly mol ng but Sterols I
ESULTS NTRODUCTION in wasp- basal dependent loads pollen; specialist among C27-phytosterols by pollen of modifica on The Two Highligh ng trlc ops n f pollen of composi on Sterolic seven seven generalist degree All plants. different obs terrestris Bombus ATERIAL
order ins tute of Mons of zoology meli d variable are prime ; supplementa on of biosciences family plant
the AND pollen o opr flrl pollen floral compare to
solitary of bee bees
AND bees
Sterol food families . bee can different
of the f the of
transi on. C. halophilus DISCUSSION these species
(i.e. polylec c). bees sterols
: we not
A. tripolium among
METHODS species
significantly wild specializa on nutrients
seem selected
bee bee ? add be that
during
plant from ZERCK Pierre-Laurent*; MICHEZ Denis; LOGNAY Georges; VANDERPLANCK Maryse variability and on the specialist DO floral
. clade, and to synthesized
2-htseo in C27-phytosterol oae on forage
A. vaga species : : in pollen. six models
:
be
foraging S. caprea
BEE differ origin ps mellifera Apis important for Meli dae, a OF different species ;
of pollen M bees ipa a display generalist
. of probably from POLLEN FEMALES
AKING trip: de novo collected They
, from bees our
S families they with
PECIALIST their are
:
sterol target
three
bees played high can their bee host’ floral C. succinctus by by needed
as ; BETTER
specialist Experiment Hypothesis development Ques on S C. vulgaris
profiles bees
LOADS constraint is TUDY pollen
MODELS a host- MODIFY three species Plant role for As . : : how do polylec c
pollen cell
: their
: floral
AND Behavior Hypochaeris radicata Hypochaeris radicata Hypochaeris radicata Hypochaeris radicata membranes and FOOD DURING M. leporina ? Comparison Lythrum salicaria Lythrum salicaria Lythrum salicaria Medicago sativa Medicago sativa Medicago sativa Calluna vulgaris Calluna vulgaris Calluna vulgaris Aster tripolium Aster tripolium Aster tripolium Hedera helix Hedera helix Hedera helix Salix caprea Salix caprea Salix caprea
M. sa va contents are
loads
THEIR STEROL choices 0.0 ß-sitosterol ∂5-avenasterol; and campesterol cholestenone; 24-methylenecholesterol of 0.1 Height ∂7-avenasterol and floral pollen. cholesterol Polylec c species 0.2
Bombus FOR HOST sterol Floral pollen Apis ∂7-stigmasterol 0.3 pollen of Specialist
of 0.4 pollen FORAGING
pollen 0.5 bees
PLANTS
supplementa on 0.6 load COMPOSITION sterol LARVAE C. hederae load load
cope Bray-Curtis dissimilarity index H. helix
otn of content
0.0 0.2 0.4 0.6 0.8
with Cholesterol addition Dasypoda hirtipes Cholestenone addition - (ISTD :
Sterols for quan fica on iden fica on (RT sterol - ? GC-FID analyses Melitta leporina in
(n=5) Colletes halophilus method D. hir pes method Modification extrac ons their varia on to (n=3)
H. radicata b generalist
Bee species Apis mellifera
(n=2) addition peculiar iosciences )
Bombus terrestris No pollen
) and Host-dependent (n=21) modification Melitta nigricans
(n=19)
Colletes hederae pollen loads UMONS RESEARCH INSTITUTE
ensure Colletes succinctus (n=3) M. nigricans Hh Ls Sc ∂5-avena. At Ms Hr Ls Sc ∂5-avena. At No modification - of 4 matrices
: : -
: s gma. :
: (n=3) : chol chol
chol Comparison chol chol
L. salicaria . Crea on data set
Andrena vaga FOR BIOSCIENCES . ., . ., . desmo
desmo (n=3)
larvae loads ., ., ß-sito (n=4) ß-sito of ., ., ,