B-7000 Mons 23, place du parc 0032 (0) 65 37 38 03 * Laboratory Research University - Impact of pollen - - - Modificaon of pollen - Pollen profiles: R [email protected] - - - collected In M

generalist highly molng but Sterols I

ESULTS NTRODUCTION in wasp- basal dependent loads pollen; specialist among C27-phytosterols by pollen of modificaon The Two Highlighng trlc opsn f pollen of composion Sterolic seven seven generalist degree All plants. different obs terrestris Bombus ATERIAL

order instute of Mons of zoology melid variable are prime ; supplementaon of biosciences family plant

the AND pollen o opr flrl pollen floral compare to

solitary of bee bees

AND bees

Sterol food families . bee can different

of the f the of

transion. C. halophilus DISCUSSION these species

(i.e. polylecc). bees sterols

: we not

A. tripolium among

METHODS species

significantly wild specializaon nutrients

seem selected

bee bee ? add be that

during

plant from ZERCK Pierre-Laurent*; MICHEZ Denis; LOGNAY Georges; VANDERPLANCK Maryse variability and on the specialist DO floral

. clade, and to synthesized

2-htseo in C27-phytosterol oae on forage

A. vaga species : : in pollen. six models

:

be

foraging S. caprea

BEE differ origin ps mellifera Apis important for Melidae, a OF different species ;

of pollen M bees ipa a display generalist

. of probably from POLLEN FEMALES

AKING trip: de novo collected They

, from bees our

S families they with

PECIALIST their are

:

sterol target

three

bees played high can their bee host’ floral C. succinctus by by needed

as ; BETTER

specialist Experiment Hypothesis development Queson S C. vulgaris

profiles bees

LOADS constraint is TUDY pollen

MODELS a host- MODIFY three species Plant role for As . : : how do polylecc

pollen cell

: their

: floral

AND Behavior Hypochaeris radicata Hypochaeris radicata Hypochaeris radicata Hypochaeris radicata membranes and FOOD DURING M. leporina ? Comparison Lythrum salicaria Lythrum salicaria Lythrum salicaria Medicago sativa Medicago sativa Medicago sativa vulgaris Calluna vulgaris Calluna vulgaris Aster tripolium Aster tripolium Aster tripolium helix Hedera helix Hedera helix Salix caprea Salix caprea Salix caprea

M. sava contents are

loads

THEIR STEROL choices 0.0 ß-sitosterol ∂5-avenasterol; and campesterol cholestenone; 24-methylenecholesterol of 0.1 Height ∂7-avenasterol and floral pollen. cholesterol Polylecc species 0.2

Bombus FOR HOST sterol Floral pollen Apis ∂7-stigmasterol 0.3 pollen of Specialist

of 0.4 pollen FORAGING

pollen 0.5 bees

PLANTS

supplementaon 0.6 load COMPOSITION sterol LARVAE C. hederae load load

cope Bray-Curtis dissimilarity index H. helix

otn of content

0.0 0.2 0.4 0.6 0.8

with Cholesterol addition Dasypoda hirtipes Cholestenone addition - (ISTD :

Sterols for quanficaon idenficaon (RT sterol - ? GC-FID analyses Melitta leporina in

(n=5) halophilus method D. hirpes method Modification extracons their variaon to (n=3)

H. radicata b generalist

Bee species Apis mellifera

(n=2) addition peculiar iosciences )

Bombus terrestris No pollen

) and Host-dependent (n=21) modification Melitta nigricans

(n=19)

Colletes hederae pollen loads UMONS RESEARCH INSTITUTE

ensure Colletes succinctus (n=3) M. nigricans Hh Ls Sc ∂5-avena. At Ms Hr Ls Sc ∂5-avena. At No modification - of 4 matrices

: : -

: sgma. :

: (n=3) : chol chol

chol Comparison chol chol

L. salicaria . Creaon data set

Andrena vaga FOR BIOSCIENCES . ., . ., . desmo

desmo (n=3)

larvae loads ., ., ß-sito (n=4) ß-sito of ., ., ,