DATA SHEET Rev 031910F

Mucin 6 (MUC-6) Ab-1 (Clone CLH5) Mouse Monoclonal Antibody Cat. #MS-1153-S0, -S1, or -S (0.1ml, 0.5ml, or 1.0ml Supernatant) Cat. #MS-1153-R7 (7.0ml) (Ready-to-Use for Immunohistochemical Staining) Cat. #MS-1153-PCS (5 Slides) (Positive Control for Histology) Please note this data sheet has been changed effective Mar 19, 2010 Description: 6 (Muc-6 ). Supplied As: Tissue culture supernatant with are heavily glycosylated , 0.09% sodium azide, or Prediluted antibody which is which constitute the major components of mucus ready-to-use for staining of formalin-fixed, paraffin- which cover the surface of epithelial tissues. Nine embedded tissues. distinct epithelial mucin (Muc-1, 2, 3, 4, 5AC, 5B, 6,7 and 8) have been identified. Various Key References: immunohistochemical and in situ hybridization 1. Reis C A, et al. Cancer Res. 59: 1003-1007 (1999). studies have shown that these mucins are differentially expressed in epithelia with cell-type Limitations and Warranty: specificity. Muc-5AC and Muc-6 glycoproteins are Our products are intended FOR RESEARCH USE the first two mucins found in superficial epithelium ONLY and are not approved for clinical diagnosis, and Muc-6 glycoprotein is found in the deep glands. drug use or therapeutic procedures. No products are to be construed as a recommendation for use in Comments: Ab-1 will be of use in the detection of violation of any patents. We make no representations, Muc-6 glycoprotein where low levels of expression warranties or assurances as to the accuracy or may be an indicator of intestinal metaplasia. completeness of information provided on our data sheets and website. Our warranty is limited to the Epitope: Not determined actual price paid for the product. NeoMarkers is not Species Reactivity: . Weakly react with liable for any property damage, personal injury, time or effort or economic loss caused by our products. Rat. Others-not known. Clone Designation: CLH5 Material Safety Data: Ig Isotype: IgG1 This product is not licensed or approved for Immunogen: Synthetic peptide of the tandem administration to or to animals other than the repeat sequence of human Muc-6 . experimental animals. Standard Laboratory Practices should be followed when handling this material. The Applications and Suggested Dilutions: chemical, physical, and toxicological properties of • Immunohistology (Formalin/paraffin) this material have not been thoroughly investigated. (Use Ab at 1:25-1:50 for 20 min at RT using Appropriate measures should be taken to avoid skin UltraVision LP Systems) and eye contact, inhalation, and ingestion. The * [Staining of formalin-fixed tissues REQUIRES boiling material contains 0.09% sodium azide as a tissue sections in 10mM citrate buffer, pH 6.0, preservative. Although the quantity of azide is very (NEOMARKERS’ Cat. #AP-9003), for 10-20 min small, appropriate care should be taken when followed by cooling at RT for 20 min.]\ handling this material as indicated above. The * Requires use of a high-sensitivity detection system National Institute of Occupational Safety and Health such as UltraVision LP (Cat# TL-015) has issued a bulletin citing the potential explosion The optimal dilution for a specific application should hazard due to the reaction of sodium azide with be determined by the investigator. copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic Positive Control: Stomach conditions and should be discarded in a large volume of running water to avoid deposits forming in metal Cellular Localization: Cytoplasmic and cell drainage pipes. surface Storage and Stability: Store vial at 4°C. When For Research Use Only stored at 2-8°C, this antibody is stable for 24 months. Thermo Fisher Scientific Thermo Fisher Scientific Anatomical Pathology Anatomical Pathology 46360 Fremont Blvd. Manufactured by: 93-96 Chadwick Road, Astmoor Fremont, CA 94538, USA Runcorn, Cheshire WA7 1PR, UK NeoMarkers Tel: 1-510-771-1560 For Tel: 44-1928-562600 Fax: 1-510-771-1570 Lab Vision Corporation Fax: 44-1928-562627 http://www.thermo.com/labvision [email protected]