All You Need for Myeloid Cell Research

Product quick guide All you need for myeloid cell research

Tissue dissociation & cell separation

Cell culture & stimulation

Flow cytometry

Germany/Austria Benelux Italy Singapore Switzerland Miltenyi Biotec B.V. & Co. KG Miltenyi Biotec B.V. Miltenyi Biotec S.r.l. Miltenyi Biotec Asia Pacific Pte. Ltd. Miltenyi Biotec Swiss AG Friedrich-Ebert-Str. 68 Sandifortdreef 17 Via Paolo Nanni Costa, 30 100 Beach Road Gibelinstrasse 27 51429 Bergisch Gladbach 2333 ZZ Leiden 40133 Bologna #28-06 to 28-08 Shaw Tower 4500 Solothurn Germany The Netherlands Italy Singapore 189702 Switzerland Phone +49 2204 8306-0 [email protected] Phone +39 051 6 460 411 Phone +65 6238 8183 Phone +41 32 623 08 47 Fax +49 2204 85197 Customer service Fax +39 051 6 460 499 Fax +65 6238 0302 Fax +49 2204 85197 [email protected] The Netherlands [email protected] [email protected] [email protected] Phone 0800 4020120 USA/Canada Fax 0800 4020100 Japan South Korea United Kingdom Miltenyi Biotec Inc. Customer service Belgium Miltenyi Biotec K.K. Miltenyi Biotec Korea Co., Ltd. Miltenyi Biotec Ltd. 2303 Lindbergh Street Phone 0800 94016 Nittsu-Eitai Building 5F Arigi Bldg. 8F Almac House, Church Lane Auburn, CA 95602, USA Fax 0800 99626 16-10 Fuyuki, Koto-ku 562 Nonhyeon-ro Bisley, Surrey GU24 9DR, UK Phone 800 FOR MACS Customer service Luxembourg Tokyo 135-0041, Japan Gangnam-gu Phone +44 1483 799 800 Phone +1 530 888 8871 Phone 800 24971 Phone +81 3 5646 8910 Seoul 06136, South Korea Fax +44 1483 799 811 Fax +1 877 591 1060 Fax 800 24984 Fax +81 3 5646 8911 Phone +82 2 555 1988 [email protected] [email protected] [email protected] Fax +82 2 555 8890 China [email protected] www.miltenyibiotec.com Australia Miltenyi Biotec Technology & Nordics and Baltics Miltenyi Biotec Trading (Shanghai) Co., Ltd. Miltenyi Biotec Norden AB Spain Australia Pty. Ltd. Room 401 Scheelevägen 17 Miltenyi Biotec S.L. Unit 11, 2 Eden Park Drive No. 1077, Zhangheng Road 223 70 Lund C/Luis Buñuel 2 Macquarie Park, NSW 2113 Pudong New Area Sweden Ciudad de la Imagen Australia 201203 Shanghai, P.R. China [email protected] 28223 Pozuelo de Alarcón (Madrid) Phone +61 2 8877 7400 Phone +86 21 6235 1005 Customer service Sweden Spain Fax +61 2 9889 5044 Fax +86 21 6235 0953 Phone 0200 111 800 Phone +34 91 512 12 90 [email protected] [email protected] Fax 046 280 72 99 Fax +34 91 512 12 91 Customer service Denmark [email protected] France Phone 80 20 30 10 Miltenyi Biotec SAS Fax +46 46 280 72 99 10 rue Mercoeur Customer service 75011 Paris, France Norway, Finland, Iceland, Phone +33 1 56 98 16 16 and Baltic countries Fax +33 1 56 98 16 17 Phone +46 46 280 72 80 [email protected] Fax +46 46 280 72 99

Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.

This publication is provided for information purposes only, and the contents hereof are subject to change without notice. While Miltenyi Biotec has taken great care and attention to ensure the quality of the information contained herein at the time of publication, no warrwanties are provided that the content of this publication is accurate, complete, reliable, up to date, and error-free. Miltenyi Biotec assumes no responsibility or liability arising out of the application or use of any information described herein except as expressly confirmed in writing by authorized representative of Miltenyi Biotec. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, MACSxpress, StraightFrom, and the MACS logo are registered trademarks or trademarks of Miltenyi Biotec B.V. & Co. KG and/or its affiliates. All other trademarks mentioned in this document are the property of their respective owners and are used for identification

purposes only. Leukopak is a registered trademark of StemExpress, LCC. Copyright © 2019 Miltenyi Biotec B.V. & Co. KG and/or its affiliates. All rights reserved. 130-120-397.04 Mouse myeloid cells from spleen and lymph nodes

CD3+/– F4/80+/– CD19+/– CD115 +/– Spleen Dissociation Kit CD49b (DX5)+/– Ly-6G+/– CD11b +/– Siglec-F+/– CD11c +/– CD200R3+/– Spleen

CD11b CD115 Myeloid-Derived CD11c MicroBeads MicroBeads MicroBead Kit Suppressor Cell UltraPure Anti-Ly-6G MicroBeads Isolation Kit UltraPure Pan DC Isolation Kit Pan DC MicroBeads

Myeloid cells (mix) Monocytes Neutrophils Eosinophils Basophils M-MDSCs G-MDSCs pan DCs

CD11b + CD64+/– CD11b + CD11b + CD11b + CD200R3+ Ly-6C+ Ly-6G+ CD11c + CD4+/– Tissue dissociation & cell separation CD11c +/– F4/80+/– CD115 + Ly-6G+ Siglec-F+ CD49b (DX5)+ CD115 + CD11b + MHC class IIhigh CD8+/– Ly-6G+/– Ly-6C+/– Ly-6C+ CD115 – CD193 (CCR3)+ FcεRIα+ CD192 (CCR2)+ CD11b +/– XCR1+/– Siglec-F+/– CD115 +/– CD192 (CCR2)+ Gr-1+ CD193 (CCR3)+ CD172a (SIRPα)+/– F4/80– CD64+ CD117– Cell culture & stimulation Mer (MerTK)–

Anti-F4/80 CD8+ Dendritic Cell Isolation Kit CD4+ Dendritic Plasmacytoid DC Isolation Kit MicroBeads Anti-XCR1 MicroBead Kit Cell Isolation Kit Anti-mPDCA-1 MicroBeads Flow cytometry UltraPure (Spleen) Red pulp White pulp Marginal zone Metallophilic cDC1s cDC2s pDCs macrophages macrophages macrophages macrophages

+ F4/80+ F4/80– F4/80– F4/80– CD11c CD11c + CD11c int + CD11b low Mer (MerTK)+ Mer (MerTK)+ Mer (MerTK)+ MHC class II MHC class II+ MHC class IIint + CD169low CD64+ CD64+ CD64+ CD8a CD8a– CD317 (PDCA-1)+ + MHC class IIlow CD209b (SIGN-R1)+ CD169+ CD370 (CLEC9A) CD4+ Siglec-H+ + Mer (MerTK)high MARCO+ XCR1 CD11b + CD45R (B220)+ – CD64+ CD4 CD172a (SIRPα)+ CD11b – – CD172a (SIRPα)+ CD11b Ly-6Chigh CD172a (SIRPα)– CD205 (DEC205)+ CD24+ Skin draining lymph node

Spleen Dissociation Kit

CD11b MicroBeads CD11c MicroBeads UltraPure

Myeloid cells (mix) Resident cDC1s Resident cDC2s Migratory dermal Migratory dermal Migratory LCs pDCs cDC1s cDC2s

+ + high high high int CD11b + MHC class II MHC class II MHC class II MHC class II MHC class II CD11c high high + + + + CD11c +/– CD11c CD11c CD11c CD11c CD11c MHC class II + – + + + + Ly-6G+/– XCR1 CD8a XCR1 CD11b CD207 CD317 (PDCA-1) + +/– + + + + Siglec-F+/– CD8a CD4 CD370 (CLEC9A) CD197 (CCR7) CD11b Siglec-H + + – + + + CD64+/– CD370 (CLEC9A) CD11b CD11b CD172a (SIRPα) CD24 CD45R (B220) – + – + – – F4/80+/– CD4 CD172a (SIRPα) CD172a (SIRPα) CD80 CD103 CD11b – – + + + high Ly-6C+/– CD11b CD197 (CCR7) CD80 CD86 F4/80 Ly-6C – + – – CD115 +/– CD172a (SIRPα) CD86 CD8a CX3CR1 CD205 (DEC205)+ CD197 (CCR7)+ CD80+ CD197 (CCR7)– CD24+ CD86+ CD24+ CD103+ CD197 (CCR7)+

Legend

separation DC: dendritic cell – no expression high high expression dissociation cDC: conventional or myeloid DC low low expression +/– expression dependent pDC: plasmacytoid DC int intermediate expression on subset MACS® Sample Preparation products (RUO) M-MDSC: monocytic myeloid-derived suppressor cell + expression MACS Cell Separation products (RUO) G-MDSC: granulocytic myeloid-derived suppressor cell MACS Cell Culture and Stimulation products (RUO) LC: Langerhans cells MACS Flow Cytometry products (RUO) Mouse myeloid cells from bone marrow and tumor

CD19+/– Ly-6G+/– CD3+/– CD115 +/– CD49b (DX5)+/– CD135+/– Sca-1+/– CD117+/– Bone marrow CD11b +/– Siglec-F+/– CD11c +/–

M-CSF GM-CSF, Flt3-L, IL-4, TNF-α Monocyte Isolation Kit (BM) CD115 MicroBead Kit CD117 CD11b Anti-Ly-6G MicroBeads MicroBeads MicroBeads UltraPure Neutrophil Isolation Kit

Macrophages BM-DCs Classical Non-classical CDPs MDPs CMoPs Myeloid Neutrophils Eosinophils Basophils monocytes monocytes cells (mix)

CD11b + MHC class II+ CD11b + CD11b + CD115 + CD115 + CD115 + CD11b + CD11b + CD11b + CD200R3+ CD11c low CD11c + CD115 + CD115 + CD117low CD117 int CD117+ Ly6C+/– Ly-6G+ Siglec-F+ CD49b (DX5)+ F4/80+ CD11b +/– CD43 (Ly-48)– CD43 (Ly-48)+ CD135+ CD135+ CD135– Siglec-F+/– Gr-1+ CD193 (CCR3)+ FcεRIα+ CD68+ CD8a+/– CD192 (CCR2)+ CD192 (CCR2)– MHC class IIlow Ly-6C– Ly-6C+ Ly-6G+/– CD115 – CD193 (CCR3)+ CD206+ F4/80– Ly-6Chigh Ly-6Clow to int CD11c low CD11c +/– CD117– CX3CR1+ CX3CR1high F4/80–

Tumor Dissociation Kit CD45+/– CD11b +/–

Tumor*

CD11b MicroBeads Anti-F4/80 MicroBeads Myeloid-Derived CD11c MicroBeads UltraPure Suppressor Cell UltraPure Isolation Kit Anti-Ly-6G MicroBeads UltraPure

Myeloid cells MHC class II+ MHC class II– MHC class II+ Classical M-MDSCs G-MDSCs DCs Neutrophils Eosinophils (mix) Ly-6C+ TAMs Ly-6C+ TAMs Ly-6C– TAMs monocytes (TANs)

CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD11b + CD11b + CD11b + CD11b + CD11b + CD11b + Ly-6G+ CD11c + CD11b + CD11b + CD11c +/– F4/80+ F4/80+ F4/80+ Ly-6C+ CD115 + CD11b + MHC class IIhigh Ly-6G+ Siglec-F+ F4/80+/– CD64+ CD64+ CD64+ CD192 (CCR2)+ Ly-6C+ CD11b +/– Gr-1+ CD193 (CCR3)+ +/– Ly-6G+/– Ly-6C+ Ly-6C+ Ly-6C– CD115 + CD192 (CCR2)+ CD172a (SIRPα) CD115 – +/– +/– + – + CD8a Ly-6C MHC class II MHC class II MHC class II +/– MHC class II+/– XCR1 F4/80– Ly-6C–

Legend * Please contact our technical support to know which tumor types were tested with indicated products.

separation DC: dendritic cell CMoP: common monocyte progenitor – no expression differentiation cDC: conventional or myeloid DC M-MDSC: monocytic myeloid-derived low low expression dissociation pDC: plasmacytoid DC suppressor cell int intermediate expression BM: bone marrow G-MDSC: granulocytic myeloid-derived + expression MACS® Sample Preparation products (RUO) BM-DC: bone marrow–derived DC suppressor cell high high expression MACS Cell Separation products (RUO) CDP: common dendritic cell progenitor LC: Langerhans cell +/– expression dependent MACS Cell Culture and Stimulation products (RUO) MDP: monocyte and DC progenitor TAM: tumor-associated macrophage on subset MACS Flow Cytometry products (RUO) TAN: tumor-associated neutrophil

Miltenyi Biotec B.V. & Co. KG | Friedrich-Ebert-Straße 68 | 51429 Bergisch Gladbach | Germany | Phone +49 2204 8306-0 | Fax +49 2204 85197 [email protected] | www.miltenyibiotec.com Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact. miltenyibiotec.com This publication is provided for information purposes only, and the contents hereof are subject to change without notice. While Miltenyi Biotec has taken great care and attention to ensure the quality of the information contained herein at the time of publication, no warranties are provided that the content of this publication is accurate, complete, reliable, up to date, and error-free. Miltenyi Biotec assumes no responsibility or liability arising out of the application or use of any information described herein except as expressly confirmed in writing by authorized representative of Miltenyi Biotec. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, MACSxpress, StraightFrom, and the MACS logo are registered trademarks or trademarks of Miltenyi Biotec B.V. & Co. KG and/or its affiliates in various countries worldwide. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Leukopak is a registered trademark of StemExpress, LCC. Copyright © 2019 Miltenyi Biotec B.V. & Co. KG and/or its affiliates. All rights reserved. Mouse myeloid cells from lung and liver

CD45+/– CD31+/– +/– Lung CD326 (EpCAM) +/– Dissociation Kit CD11c CD11b +/– F4/80+/– Lung Ly-6G+/– MHC class II+/– CD193 (CCR3)+/–

mPDCA-1 MicroBeads CD11c MicroBeads Anti-Siglec-F Anti-Ly-6G Anti-Siglec-F UltraPure MicroBeads MicroBeads MicroBeads UltraPure pDCs Monocytes Alveolar Interstitial Neutrophils Eosinophils Basophils macrophages macrophages cDC1s cDC2s

CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ MHC class IIint CD11b + F4/80+ CD11c– CD11b + CD11b + CD200R3+ CD317 (PDCA-1)+ CD45+ CD45+ CD11c– CD64+ F4/80+ Ly-6G+ Siglec-F+ CD49b+ CD45R (B220)+ MHC class II+ MHC class II+ Mer (MerTK)– CD11b low CD64+ CD115 – CD193 (CCR3)+ FcεRIα+ Siglec-H+ CD11c + CD11c + CD64int CD11c high MHC class II+ Gr-1+ CD193 (CCR3)+ CD11b – CD103+ CD11b high CD192 (CCR2)+ Siglec-F+ CD11b + Ly-6C– CD117– Ly-6Chigh XCR1+ CD172 (SIRPα)+ Ly-6Chigh Siglec-F– CD11c low CD370 (CLEC9A)+ CD103– MHC class II– CD11b low CD64– CX3CR1+ CD64– F4/80–

Liver CD45+/– Dissociation Kit CD146+/– CD326 (EpCAM)+/– Liver CD11b +/–

Anti-Siglec-F MicroBeads Anti-Ly-6G MicroBeads CD11b MicroBeads UltraPure Anti-F4/80 MicroBeads UltraPure

Eosinophils Neutrophils Kupffer cells

CD45+ CD45+ CD45+ Siglec-F+ Ly-6G+ CD11b low CD193 (CCR3)+ Gr-1+ F4/80+ CD11b + CD11b + CD64+ Ly-6G– CX3CR1– CD172 (SIRPα)+ Mer (MerTK)+

Legend

separation DC: dendritic cell – no expression dissociation cDC: conventional or myeloid DC low low expression pDC: plasmacytoid DC int intermediate expression MACS® Sample Preparation products (RUO) + expression MACS Cell Separation products (RUO) high high expression MACS Cell Culture and Stimulation products (RUO) +/– expression dependent on subset MACS Flow Cytometry products (RUO) Mouse myeloid cells from lamina propria and peritoneum

Lamina Propria CD45+/– Dissociation Kit CD11b +/– CD11c +/– CD64+/– Lamina propria (CD45 MicroBeads) CD11c MicroBeads UltraPure

pDCs CD103+ CD11b – CD103+ CD11b + CD103– CD11b + Monocytes Mo-DCs Lamina propria Neutrophils cDC1s cDC2s cDCs macrophages

CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ MHC class IIint MHC class II+ MHC class II+ MHC class II+ CD11b + CD11b + CD11b + CD11b + CD11c int CD11c + CD11c + CD11c + Mer (MerTK)– CD11c int to+ CD11c low Ly-6G+ Ly-6C+ CD103+ CD103+ CD103– CD64int Mer (MerTK)– Mer (MerTK)+ Gr-1+ CD317 (PDCA-1)+ XCR1+ CD11b + CD11b + CD192 (CCR2)+ CD64+ CD64+ CD115 – CD64– CD370 (CLEC9A)+ CD172a (SIRPα)+ CD172a (SIRPα)+ Ly-6C+ CD192 (CCR2)+ CD192 (CCR2)+ CD11b – XCR1– XCR1– MHC class II– Ly-6Clow Ly-6Clow CD8a+ CD370 (CLEC9A)– CD370 (CLEC9A)– CX3CR1+ MHC class II+ MHC class II+ CD64– CD64– CD64– CX3CR1+ CD172 (SIRPα)-

CD11b +/– CD11c +/– MHC class II+/– F4/80+/–

Peritoneum

Macrophage Isolation Kit Anti-Ly-6G MicroBeads (Peritoneum) UltraPure

Large peritoneal Small peritoneal Neutrophils Eosinophils macrophages macrophages

CD11b + CD11b + CD11b + CD11b + F4/80+ F4/80– to low Ly-6G+ Siglec-F+ MHC class IIlow to int MHC class II+ Gr-1+ CD193 (CCR3)+ CD226 (DNAM-1)– CD226 (DNAM-1)+ CD115 – TIM-4+ TIM-4–

Legend

separation DC: dendritic cell – no expression dissociation cDC: conventional or myeloid DC low low expression pDC: plasmacytoid DC int intermediate expression MACS® Sample Preparation products (RUO) Mo-DC: monocyte-derived DC + expression MACS Cell Separation products (RUO) high high expression MACS Cell Culture and Stimulation products (RUO) +/– expression dependent on subset MACS Flow Cytometry products (RUO) Mouse myeloid cells from skin and brain

CD45+/– Multitissue Epidermal CD11c +/– Dissociation Kit 1 Dissociation Kit CD64+/– CD11b +/– CD24+/– CD103+/– Skin Epidermal Langerhans Cell (CD45 MicroBeads) MicroBead Kit CD207 (Langerin) MicroBeads Anti-Ly-6G CD115 F4/80 MicroBeads CD11c MicroBeads UltraPure MicroBeads UltraPure MicroBead Kit UltraPure

Neutrophils Monocytes Mo-DCs Dermal Dermal XCR1+ Dermal CD11b+ Dermal DN DCs Langerhans macrophages cDC1s cDC2s cells

CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD45+ CD11b + CD11b + CD11b + CD11b + MHC class II+ MHC class II+ MHC class II+ MHC class II+ Ly-6G+ CD11c– CD11c int CD11c low CD11c + CD11c + CD11c + CD11c + Gr-1+ Mer (MerTK)– Mer (MerTK)– Mer (MerTK)+ CD103+ CD103– CD64– CD207 (Langerin)+ CD115 – CD64int CD64+ CD64+ XCR1+ CD11b + CD103– CD11b + Ly-6C– CD192 (CCR2)+ CD192 (CCR2)+ F4/80int CD370 (CLEC9A)+ CD172a (SIRPα)+ XCR1– CD205 (DEC205)+ Ly-6Chigh Ly-6Clow CD192 (CCR2)– CD11b – XCR1– CD11b – CD103– MHC class II– MHC class II+ Ly-6Clow CD8a+ CD370 (CLEC9A)– F4/80+ CX3CR1+ CX3CR1+ MHC class II+/– CD64– CD8alow CX3CR1– CX3CR1– CD24+ CD64– CD24+

Neural Tissue Dissociation Kit CD45+/– Adult Brain Dissociation Kit CD11b +/– Brain Tumor Dissociation Kit ACSA-2+/– Multitissue Dissociation Kit 1 Brain

CD11b (Microglia) MicroBeads

Microglia

CD45Int CD11b + CD64+ CCR2– CD45int CX3CR1high F480+

Legend

separation DC: dendritic cell – no expression high high expression dissociation cDC: conventional or myeloid DC low low expression +/– expression dependent Mo-DC: monocyte derived DC int intermediate expression on subset MACS® Sample Preparation products (RUO) Dermal DN: Dermal double negative + expression MACS Cell Separation products (RUO) MACS Cell Culture and Stimulation products (RUO) MACS Flow Cytometry products (RUO)

Peripheral Whole blood Buffy coat LRSC Leukopak® Density blood gradient

CD16 Eosinophil MicroBeads Isolation Kit PBMCs CD16 MicroBeads (depletion) StraightFrom® StraightFrom® StraightFrom® StraightFrom® Whole Blood Buffy Coat LRSC Leukopak® Neutrophils Eosinophils CD14 MicroBeads CD14 MicroBead CD14 MicroBead CD14 MicroBead Kit Kit Kit

+ + + CD15 CD15 CD66b + + CD16− CD193 (CCR3)+ CD45 CD16 +/− + Siglec-8+ CD14 CD66b +/− CD177+/− CD15 HLA-DR+ CD3+/− + StraightFrom Whole Blood MACSxpress® Whole MACSxpress CD14 MicroBeads Pan Monocyte CD16 Monocyte CD19+/− CD15 MicroBeads Blood Neutrophil Whole Blood CD14 MicroBeads UltraPure Isolation Kit Isolation Kit CD56+/− StraightFrom Whole Blood Isolation Kit Eosinophil Isolation Kit Classical Monocyte CD66b MicroBeads Isolation Kit

Eosinophils and Neutrophils Eosinophils Classical Pan monocytes Non-classical Neutrophils monocytes monocytes

CD15+ CD15+ CD15+ CD14high CD14+/int CD14int CD66b+ CD16+ CD16− CD16− CD16+/− CD16high CD11b + CD66b+ Siglec-8+ CD15− CD15− CD15− CD16+/− CD177+/− CD66b+ HLA-DR+ HLA-DR+ HLA-DR+ + CD181 (CXCR1) CD193 (CCR3)+ + CD182 (CXCR2) CD116 + CD11b +

M-CSF/IL-34 + RANKL GM-CSF/IFN-γ + LPS M-CSF IL-4/GM-CSF IL-4/IL-13 IC + TLR-Ligands IL-10/TGF-β Osteoclasts Mo-macrophages Mo-macrophages Mo-macrophages Mo-macrophages Mo-DCs (M1-like) (M2a-like) (M2b-like) (M2c-like)

Cathepsin K+ HLA-DR+ CD163+ HLA-DR+ CD206+ CD14− MMP-9+ CD80+ CD206+ CD86+ CD150 (SLAM)+ CD11b + CD86+ Arg1+ iNOS+ Arg1+ CD209 (DC-SIGN)high HLA-DR+

CD141 (BDCA-3) CD1c (BDCA-1) DC Diamond Plasmacytoid Blood DC Slan (M-DC8) CD15 HLA-DR+ Basophil Isolation CD11b MicroBead Kit Isolation Kit DC Isolation Kit II Isolation Kit II Monocyte MicroBeads MicroBeads + Kit II MicroBeads Myeloid DC Myeloid DC Plasmacytoid DC Pan-DC Isolation Kit CD14 MicroBeads Diamond Basophil Isolation Kit Isolation Kit Isolation Kit II Enrichment Kit Isolation Kit CD304 (BDCA-4/ Neuropilin-1) CD123 MicroBeads MicroBead Kit

CD141+ cDC1s CD1c+ cDC2s pDCs Pan DCs Slan (M-DC8) DCs G-MDSCs M-MDSCs Basophils Myeloid cells (mix)

HLA-DR+ HLA-DR+ HLA-DR+ HLA-DR+ HLA-DR+ CD15+ HLA-DRlow CD203c+ CD11c +/− CD141 (BDCA-3)+ CD172a (SIRPα)+ CD303 (BDCA-2)+ CD3− Slan (M-DC8)+ CD66b+ CD14+ CD123+ CD14+/− CD370 (CLEC9A)+ CD1c (BDCA-1)+ CD304 (BDCA-4)+ CD14− CD11c + LOX-1+ CD33+ aIgE+ CD16+/− XCR1+ CD11b + CD123 (IL-3R)high CD19− CD14int CD11b + CD11b + CD33+ CD123 (IL-3R)− CD123 (IL-3R)int CD85g (ILT7)+ CD56− CD16high CD15− CD11c + CD11c high CD11c low CX3CR1high CD11b low CD115 +

Legend

separation DC: dendritic cell M-MDSC: monocytic myeloid- − no expression high high expression differentiation (cultivation) cDC: conventional or myeloid DC derived suppressor cell low low expression +/− expression dependent pDC: plasmacytoid DC G-MDSC: granulocytic myeloid- int intermediate expression on subset MACS® Cell Separation products (RUO) Mo-DC: monocyte-derived DC derived suppressor cell + expression MACS Cell Culture and Stimulation products (RUO) PBMC: peripheral blood mononuclear cell IC: immunocomplexes MACS Flow Cytometry products (RUO) miltenyibiotec.com

Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.