RESEARCH ARTICLE Double Virus Vector Infection to the Prefrontal Network of the Macaque Brain Mineki Oguchi1☯, Miku Okajima2☯, Shingo Tanaka1, Masashi Koizumi1, Takefumi Kikusui3, Nobutsune Ichihara3, Shigeki Kato4, Kazuto Kobayashi4, Masamichi Sakagami1* 1 Brain Science Institute, Tamagawa University, Machida, Tokyo, Japan, 2 Graduate School of Arts and Sciences, The University of Tokyo, Meguro, Tokyo, Japan, 3 School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan, 4 Department of Molecular Genetics, Institute of Biomedical Sciences, Fukushima Medical University, Fukushima, Fukushima, Japan a11111 ☯ These authors contributed equally to this work. *
[email protected] Abstract To precisely understand how higher cognitive functions are implemented in the prefrontal OPEN ACCESS network of the brain, optogenetic and pharmacogenetic methods to manipulate the signal Citation: Oguchi M, Okajima M, Tanaka S, Koizumi transmission of a specific neural pathway are required. The application of these methods, M, Kikusui T, Ichihara N, et al. (2015) Double Virus however, has been mostly restricted to animals other than the primate, which is the best ani- Vector Infection to the Prefrontal Network of the mal model to investigate higher cognitive functions. In this study, we used a double viral Macaque Brain. PLoS ONE 10(7): e0132825. doi:10.1371/journal.pone.0132825 vector infection method in the prefrontal network of the macaque brain. This enabled us to express specific constructs into specific neurons that constitute a target pathway without Editor: Joseph Charles Glorioso, University of Pittsburgh School of Medicine, UNITED STATES use of germline genetic manipulation. The double-infection technique utilizes two different virus vectors in two monosynaptically connected areas.