1／22 2／22 Supplementary Figure S1. (A) View of the upper surface of the pathogen on PDA 7 days after plating. (B) View from the underside of a pure culture on PDA 7 days after plating. (C) Conidia of C. siamense; scale bar = 20 mm. (D) Germinated conidia developing new conidia; scale bar = 20 mm. (E) Appressoria developing directly from germ tubes; scale bar = 10 mm. (F) Unwounded leaf showing anthracnose symptoms 9 days post inoculation with isolate RS29 (left side of leaf) compared with the asymptomatic control inoculation (right side of leaf); Symptoms on (G) stems, (H) receptacles, and (I) calyxes of R. chinensis observed on wounded tissues 3 days post inoculation. Corresponding controls remained asymptomatic.

3／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Supplementary Figure 2. Phylogentic tree constructed using MEGA7 based on neighbor-joining of the concatenated data from sequences of ITS, CAL, GAPDH, ACT, GS and beta-TUB-2 of the three isolates described in this study (RS29, RS30, and RS35).

4／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN

5／22

https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS29 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence

GenBank: MH643994.1 FASTA Graphics Go to: LOCUS MH643994 507 bp DNA linear PLN 10-FEB-2019 DEFINITION Colletotrichum siamense isolate RS29 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. ACCESSION MH643994 VERSION MH643994.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 507) AUTHORS Li,H. TITLE New Record of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in Changsha City of China JOURNAL Unpublished REFERENCE 2 (bases 1 to 507) AUTHORS Li,H. TITLE Direct Submission JOURNAL Submitted (19-JUL-2018) Forestry college, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..507 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS29" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="18-Apr-2018" /collected_by="He Li" /identified_by="He Li" misc_RNA <1..>507 /note="contains internal transcribed spacer 1, 5.8S ribosomal RNA, internal transcribed spacer 2, and large subunit ribosomal RNA" ORIGIN 1 attactgagt ttacgctcta caaccctttg tgaacatacc tataactgtt gcttcggcgg 61 gtagggtccc cgtgaccctc ccggcctccc gcccccgggc gggtcggcgc ccgccggagg 121 ataaccaaac tctgatttaa cgacgtttct tctgagtggt acaagcaaat aatcaaaact 181 tttaacaacg gatctcttgg ttctggcatc gatgaagaac gcagcgaaat gcgataagta 241 atgtgaattg cagaattcag tgaatcatcg aatctttgaa cgcacattgc gcccgccagc 301 attctggcgg gcatgcctgt tcgagcgtca tttcaaccct caagctctgc ttggtgttgg 361 ggccctacag ctgatgtagg ccctcaaagg tagtggcgga ccctcccgga gcctcctttg 421 cgtagtaact ttacgtctcg cactgggatc cggagggact cttgccgtaa aaccccccaa 481 ttttccaaag gttgacctcg gatcagg //

5／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS29 calmodulin gene, partial cds GenBank: MH643995.1 FASTA Graphics Go to: LOCUS MH643995 625 bp DNA linear PLN 10-FEB-2019 DEFINITION Colletotrichum siamense isolate RS29 calmodulin gene, partial cds. ACCESSION MH643995 VERSION MH643995.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 625) AUTHORS Li,H. TITLE New Record of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in Changsha City of China JOURNAL Unpublished REFERENCE 2 (bases 1 to 625) AUTHORS Li,H. TITLE Direct Submission JOURNAL Submitted (19-JUL-2018) Forestry college, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..625 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS29" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="18-Apr-2018" /collected_by="He Li" /identified_by="He Li" mRNA join(<23..38,166..291,350..420,471..>612) /product="calmodulin" CDS join(<23..38,166..291,350..420,471..>612) /note="CAL" /codon_start=1 /product="calmodulin" /protein_id="QBA55508.1" /translation="DKDGDGQITTKELGTVMRSLGQNPSESELQDMINEVDADNNGTI DFPEFLTMMARKMKDTDSEEEIREAFKVFDRDNNGFISAAELRHVMTSIGEKLTDDEV DEMIREADQDGDGRID" ORIGIN 1 ggcctttgct aacccacaca aggacaagga tggcgatggt cagtgcctca ccccttttgc 61 tccccttcga tgcgctcctg ggaacgccgt tcgcgaccgt cgatacacca gcggcattcg 121 atgccaccgt cgaggcatgt agaatcgagc taaagagttg gacaggccaa atcactacaa 181 aggagctggg caccgtcatg cgctctctcg gccagaaccc ctccgagtcg gagctccaag 241 acatgatcaa cgaggtcgac gccgacaaca acggcaccat cgactttccc ggtaggtgga 301 catgcggaat cctgcgtcac acaatacagg cccgctgact ggtcttcaga gttcctgact 361 atgatggccc gcaagatgaa ggataccgac tccgaagagg agattcgcga ggctttcaag 421 gttggctacg acggtgcttt ttcgctgcga tatatgttga ctctgaatag gtctttgacc 481 gcgacaacaa cggcttcatc tccgccgccg agttgcgtca cgtcatgacc tccattggtg 541 agaagcttac cgacgacgag gttgacgaga tgattcgcga ggccgatcag gatggcgatg 601 gacgcattga ctgtgagtgt cgatt //

6／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS29 glyceraldehyde -3-phosphate dehydrogenase gene, partial cds

GenBank: MH643996.1 FASTA Graphics Go to: LOCUS MH643996 218 bp DNA linear PLN 10-FEB-2019 DEFINITION Colletotrichum siamense isolate RS29 glyceraldehyde-3-phosphate dehydrogenase gene, partial cds. ACCESSION MH643996 VERSION MH643996.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 218) AUTHORS Li,H. TITLE New Record of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in Changsha City of China JOURNAL Unpublished REFERENCE 2 (bases 1 to 218) AUTHORS Li,H. TITLE Direct Submission JOURNAL Submitted (19-JUL-2018) Forestry college, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..218 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS29" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="18-Apr-2018" /collected_by="He Li" /identified_by="He Li" mRNA <195..>218 /product="glyceraldehyde-3-phosphate dehydrogenase" CDS <195..>218 /note="GAPDH" /codon_start=1 /product="glyceraldehyde-3-phosphate dehydrogenase" /protein_id="QBA55509.1" /translation="AYMLKYDS" ORIGIN 1 cccctccaaa ctcgccatga cttcacatcc atcaccacca ccaccgctgt catctacatc 61 tcgccacccg cgtatggtaa acaagaacgc cgtcatgaat ggaggccaat tgaaaccatg 121 ggtcgggacg gccggacaca tgctatcact catatcagcc ctatctgtca catttactga 181 ctcgctcttc acaggcctac atgctcaagt acgactcc //

7／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS29 actin gene, partial cds

GenBank: MH643997.1 FASTA Graphics Go to: LOCUS MH643997 212 bp DNA linear PLN 10-FEB-2019 DEFINITION Colletotrichum siamense isolate RS29 actin gene, partial cds. ACCESSION MH643997 VERSION MH643997.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 212) AUTHORS Li,H. TITLE New Record of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in Changsha City of China JOURNAL Unpublished REFERENCE 2 (bases 1 to 212) AUTHORS Li,H. TITLE Direct Submission JOURNAL Submitted (19-JUL-2018) Forestry college, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..212 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS29" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="18-Apr-2018" /collected_by="He Li" /identified_by="He Li" mRNA join(<87..117,187..>212) /product="actin" CDS join(<87..117,187..>212) /note="ACT" /codon_start=3 /product="actin" /protein_id="QBA55510.1" /translation="SIVGRPRHHGIMIGMGQK" ORIGIN 1 ccgcagaccg caacccgcct cttcaggggg tactcagatt tgcggtcatc aatcggcgtg 61 tgctgtcgat gctaaccacc acgtagcctc catcgtcggt cgccctcgcc accatgggta 121 tgtctgcttc tcgccctcgc tgcggtaatt tccgccctcc gcgccgcgat ctaacatgtg 181 aatcagtatc atgattggta tgggccagaa gg //

8／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN

https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS29 glutamine synthetase gene, partial cds

GenBank: MH643998.1 FASTA Graphics Go to: LOCUS MH643998 731 bp DNA linear PLN 10-FEB-2019 DEFINITION Colletotrichum siamense isolate RS29 glutamine synthetase gene, partial cds. ACCESSION MH643998 VERSION MH643998.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 731) AUTHORS Li,H. TITLE New Record of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in Changsha City of China JOURNAL Unpublished REFERENCE 2 (bases 1 to 731) AUTHORS Li,H. TITLE Direct Submission JOURNAL Submitted (19-JUL-2018) Forestry college, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..731 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS29" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="18-Apr-2018" /collected_by="He Li" /identified_by="He Li" mRNA <711..>731 /product="glutamine synthetase" CDS <711..>731 /note="GS" /codon_start=1 /product="glutamine synthetase" /protein_id="QBA55511.1" /translation="TLKEKEY" ORIGIN 1 cacgacctgc ggcggcgggc gcaacaaagc tggggaagcg gccccgtgtt ttgaggaatc 61 attgcctcgg ggtctctccg agtctgcccc ggactgagat ttaggcgggc tgctgcagca 121 ggttgcggcg acggcaagca ctggggcttg gcgggggtca aaccaccgcc tgccccgcgg 181 atgctgtgtt gggtgttggg ttggcggtcg ttgtacgtac ctgctcagct ctgctcggct 241 gggcttctgg ttattgccag tagccggggc tgctggctga caggattcgc acacgactcg 301 gttctgcaag gaaccccgcc ccacctggct ggtgcagcga ctccgaggcc tcgccttgcc 361 tccagctgca gggttcaacg cggcatctcg ggtattgcag gtcttgcaaa caaaaagtct 421 agaggtgtct ttgtccgctc gccgtcgggg cgcatccttc ttgccggttg gccagccgcc 481 ggcgcgacgt gatcgcagtt acgaccccac tggcgatggg cccgagaccc aaaagccccg 541 aatgagcgac tggagctccc cgcttgagca tcgtcagttc gacgacaagg cgcatcgcat 601 gatgtcagcc ttgcaggcta aagttggcca cgcgccgagc atttgccatg cccagacttg 661 catcacaacg ccacattcac aacatcgata ctgacaatgt gcgtctacag acactcaagg 721 agaaggagta c

9／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS29 beta-tubulin 2 gene, partial cds

GenBank: MH643999.1 FASTA Graphics Go to: LOCUS MH643999 644 bp DNA linear PLN 10-FEB-2019 DEFINITION Colletotrichum siamense isolate RS29 beta-tubulin 2 gene, partial cds. ACCESSION MH643999 VERSION MH643999.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 644) AUTHORS Li,H. TITLE New Record of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in Changsha City of China JOURNAL Unpublished REFERENCE 2 (bases 1 to 644) AUTHORS Li,H. TITLE Direct Submission JOURNAL Submitted (19-JUL-2018) Forestry college, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..644 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS29" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="18-Apr-2018" /collected_by="He Li" /identified_by="He Li" mRNA join(<135..158,217..242,314..355,411..465,537..>644) /product="beta-tubulin 2" CDS join(<135..158,217..242,314..355,411..465,537..>644) /note="tub2" /codon_start=1 /product="beta-tubulin 2" /protein_id="QBA55512.1" /translation="VHLQTGQCGNQIGAAFWQNISGEHGLDSNGVYNGTSELQLERMS VYFNEASGNKYVPRAVLVDLEPGTMDAVRAGPFGQLFRPDN" ORIGIN 1 atccgccttg cccctgagcg taccccgccg acatttttac ccgacttcta tgctcaacaa 61 acccgcgacg cctgtcaatc atcgacgtcc aactctggaa tgttttgctg actgctgctt 121 ttttttgtct acaggttcac ctccagaccg gccagtgcgt aagtcttccc aagccaaatc 181 caaccgcctg attgcggggc taacctcctt gtacagggta accagattgg tgctgccttc 241 tggtacgtga cgagaccgcc gacgatccgg caatatatac ttgcgaggac ggcagatgtt 301 gacgatagag taggcaaaac atttctggcg agcacggcct cgacagcaat ggagtgtatg 361 tcatgcccct tatctggcca cattggtggt tgaccgctaa actcgaacag ctacaacggc 421 acctctgagc tccagctcga gcgcatgagc gtctacttca acgaagtttg ttaccttata 481 gcccccagag tgcaagataa acatattgac gagtactgac cttcgctcct acccaggctt 541 ccggcaacaa gtacgtgccc cgtgccgtcc tcgtcgattt ggagcccggt accatggacg 601 ccgtccgtgc cggtcctttc ggccagctct tccgccccga caac //

10／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN

https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS30 actin (ACT) gene, partial cds

GenBank: MK446249.1 FASTA Graphics PopSet Go to: LOCUS MK446249 212 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS30 actin (ACT) gene, partial cds. ACCESSION MK446249 VERSION MK446249.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 212) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 212) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..212 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS30" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <87..>212 /gene="ACT" mRNA join(<87..117,187..>212) /gene="ACT" /product="actin" CDS join(<87..117,187..>212) /gene="ACT" /codon_start=3 /product="actin" /protein_id="QCW06509.1" /translation="SIVGRPRHHGIMIGMGQK" ORIGIN 1 ccgcagaccg caacccgcct cttcaggggg tactcagatt tgcggtcatc aatcggcgtg 61 tgctgtcgat gctaaccacc acgtagcctc catcgtcggt cgccctcgcc accatgggta 121 tgtctgcttc tcgccctcgc tgcggtaatt tccgccctcc gcgccgcgat ctaacatgtg 181 aatcagtatc atgattggta tgggccagaa gg //

11／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS35 actin (ACT) gene, partial cds

GenBank: MK446250.1 FASTA Graphics PopSet Go to: LOCUS MK446250 212 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS35 actin (ACT) gene, partial cds. ACCESSION MK446250 VERSION MK446250.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 212) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 212) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..212 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS35" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <87..>212 /gene="ACT" mRNA join(<87..117,187..>212) /gene="ACT" /product="actin" CDS join(<87..117,187..>212) /gene="ACT" /codon_start=3 /product="actin" /protein_id="QCW06510.1" /translation="SIVGRPRHHGIMIGMGQK" ORIGIN 1 ccgcagaccg caacccgcct cttcaggggg tactcagatt tgcggtcatc aatcggcgtg 61 tgctgtcgat gctaaccacc acgtagcctc catcgtcggt cgccctcgcc accatgggta 121 tgtctgcttc tcgccctcgc tgcggtaatt tccgccctcc gcgccgcgat ctaacatgtg 181 aatcagtatc atgattggta tgggccagaa gg //

12／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS30 calmodulin (CAL) gene, partial cds

GenBank: MK446251.1 FASTA Graphics PopSet Go to: LOCUS MK446251 625 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS30 calmodulin (CAL) gene, partial cds. ACCESSION MK446251 VERSION MK446251.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 625) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 625) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..625 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS30" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <23..>612 /gene="CAL" mRNA join(<23..38,166..291,350..420,471..>612) /gene="CAL" /product="calmodulin" CDS join(<23..38,166..291,350..420,471..>612) /gene="CAL" /codon_start=1 /product="calmodulin" /protein_id="QCW06511.1" /translation="DKDGDGQITTKELGTVMRSLGQNPSESELQDMINEVDADNNGTI DFPEFLTMMARKMKDTDSEEEIREAFKVFDRDNNGFISAAELRHVMTSIGEKLTDDEV DEMIREADQDGDGRID" ORIGIN 1 ggcctttgct aacccacaca aggacaagga tggcgatggt cagtgcctca ccccttttgc 61 tccccttcga tgcgctcctg ggaacgccgt tcgcgaccgt cgatacacca gcggcattcg 121 atgccaccgt cgaggcatgt agaatcgagc taaagagttg gacaggccaa atcactacaa 181 aggagctggg caccgtcatg cgctctctcg gccagaaccc ctccgagtcg gagctccaag 241 acatgatcaa cgaggtcgac gccgacaaca acggcaccat cgactttccc ggtaggtgga 301 catgcggaat cctgcgtcac acaatacagg cccgctgact ggtcttcaga gttcctgact 361 atgatggccc gcaagatgaa ggataccgac tccgaagagg agattcgcga ggctttcaag 421 gttggctacg acggtgcttt ttcgctgcga tatatgttga ctctgaatag gtctttgacc 481 gcgacaacaa cggcttcatc tccgccgccg agttgcgtca cgtcatgacc tccattggtg 541 agaagcttac cgacgacgag gttgacgaga tgattcgcga ggccgatcag gatggcgatg 601 gacgcattga ctgtgagtgt cgatt

13／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS35 calmodulin (CAL) gene, partial cds

GenBank: MK446252.1 FASTA Graphics PopSet Go to: LOCUS MK446252 625 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS35 calmodulin (CAL) gene, partial cds. ACCESSION MK446252 VERSION MK446252.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 625) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 625) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China FEATURES Location/Qualifiers source 1..625 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS35" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <23..>612 /gene="CAL" mRNA join(<23..38,166..291,350..420,471..>612) /gene="CAL" /product="calmodulin" CDS join(<23..38,166..291,350..420,471..>612) /gene="CAL" /codon_start=1 /product="calmodulin" /protein_id="QCW06512.1" /translation="DKDGDGQITTKELGTVMRSLGQNPSESELQDMINEVDADNNGTI DFPEFLTMMARKMKDTDSEEEIREAFKVFDRDNNGFISAAELRHVMTSIGEKLTDDEV DEMIREADQDGDGRID" ORIGIN 1 ggcctttgct aacccacaca aggacaagga tggcgatggt cagtgcctca ccccttttgc 61 tccccttcga tgcgctcctg ggaacgccgt tcgcgaccgt cgatacacca gcggcattcg 121 atgccaccgt cgaggcatgt agaatcgagc taaagagttg gacaggccaa atcactacaa 181 aggagctggg caccgtcatg cgctctctcg gccagaaccc ctccgagtcg gagctccaag 241 acatgatcaa cgaggtcgac gccgacaaca acggcaccat cgactttccc ggtaggtgga 301 catgcggaat cctgcgtcac acaatacagg cccgctgact ggtcttcaga gttcctgact 361 atgatggccc gcaagatgaa ggataccgac tccgaagagg agattcgcga ggctttcaag 421 gttggctacg acggtgcttt ttcgctgcga tacatgttga ctctgaatag gtctttgacc 481 gcgacaacaa cggcttcatc tccgccgccg agttgcgtca cgtcatgacc tccattggtg 541 agaagcttac cgacgacgag gttgacgaga tgattcgcga ggccgatcag gatggcgatg 601 gacgcattga ctgtgagtgt cgatt //

14／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS30 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, partial cds

GenBank: MK446253.1 FASTA Graphics PopSet Go to: LOCUS MK446253 218 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS30 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, partial cds. ACCESSION MK446253 VERSION MK446253.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 218) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 218) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..218 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS30" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <195..>218 /gene="GAPDH" mRNA <195..>218 /gene="GAPDH" /product="glyceraldehyde-3-phosphate dehydrogenase" CDS <195..>218 /gene="GAPDH" /codon_start=1 /product="glyceraldehyde-3-phosphate dehydrogenase" /protein_id="QCW06513.1" /translation="AYMLKYDS" ORIGIN 1 cccctccaaa ctcgccatga cttcacatcc atcaccacca ccaccgctgt catctacatc 61 tcgccacccg cgtttggtaa acaagaaggc cgtcatgaat ggaggccaat tgaaaccatg 121 ggtcgggacg gccggacaca tgctatcact catatcagcc ccatctgtca catttactga 181 ctcgctcttc acaggcctac atgctcaagt acgactcc //

15／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS35 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, partial cds

GenBank: MK446254.1 FASTA Graphics PopSet Go to: LOCUS MK446254 218 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS35 glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, partial cds. ACCESSION MK446254 VERSION MK446254.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 218) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 218) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..218 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS35" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <195..>218 /gene="GAPDH" mRNA <195..>218 /gene="GAPDH" /product="glyceraldehyde-3-phosphate dehydrogenase" CDS <195..>218 /gene="GAPDH" /codon_start=1 /product="glyceraldehyde-3-phosphate dehydrogenase" /protein_id="QCW06514.1" /translation="AYMLKYDS" ORIGIN 1 cccctccaaa ctcgccatga cttcacatcc atcaccacca ccaccgctgt catctacatc 61 tcgccacccg cgtttggtaa acaagaaggc cgtcatgaat ggaggccaat tgaaaccatg 121 ggtcgggacg gccggacaca tgctatcact catatcagcc ctgtctgtca catttactga 181 ctcgctcttc acaggcctac atgctcaagt acgactcc //

16／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS30 glutamine synthetase (GS) gene, partial cds

GenBank: MK446255.1 FASTA Graphics PopSet Go to: LOCUS MK446255 731 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS30 glutamine synthetase (GS) gene, partial cds. ACCESSION MK446255 VERSION MK446255.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 731) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 731) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China FEATURES Location/Qualifiers source 1..731 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS30" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <711..>731 /gene="GS" mRNA <711..>731 /gene="GS" /product="glutamine synthetase" CDS <711..>731 /gene="GS" /codon_start=1 /product="glutamine synthetase" /protein_id="QCW06515.1" /translation="TLKEKEY" ORIGIN 1 cacgacctgc ggcggcgggc gcaacaaagc tggggaagcg gccccgtgtt ttgaggaatc 61 attgcctcgg ggtctctccg agtctgcccc ggactgagat ttaggcgggc tgctgcagca 121 ggttgcggcg acggcaagca ctggggcttg gcgggggtca aaccaccgcc tgccccgcgg 181 atgctgtgtt gggtgttggg ttggcggtcg ttgtacgtac ctgctcagct ctgctcggct 241 gggcttctgg ttattgccag tagccggggc tgctggctga caggattcgc acacgactcg 301 gttctgcaag gaaccccacc ccacctggct ggtgcagcga ctctgaggcc tcgccttgcc 361 tccagctgca gggttcaacg cggcatctcg ggtattgcag gtcttgcaaa caaaaagtcc 421 agaggcgtct ttgtccgctc gccgtcgggg cgcatccttc ttgccggttg cccagccgcc 481 ggcgcgacgt gatcgcagtc acgaccccac tggcgatggg cccgagaccc aaaagccccg 541 aatgagcgac tggagctccc cgcttgagca tcgtcagttc gacgacaagg cgcatcgcat 601 gatgtcagcc ttgcaggcca aagttggcca cgcgccgagc atttgccatg cccagacttg 661 catcacaacg ccacattcac aacatcgata ctgacaatgt gcgtctacag acactcaagg 721 agaaggagta c

17／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS35 glutamine synthetase (GS) gene, partial cds

GenBank: MK446256.1 FASTA Graphics PopSet Go to: LOCUS MK446256 731 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS35 glutamine synthetase (GS) gene, partial cds. ACCESSION MK446256 VERSION MK446256.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 731) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 731) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China FEATURES Location/Qualifiers source 1..731 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS35" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <711..>731 /gene="GS" mRNA <711..>731 /gene="GS" /product="glutamine synthetase" CDS <711..>731 /gene="GS" /codon_start=1 /product="glutamine synthetase" /protein_id="QCW06516.1" /translation="TLKEKEY" ORIGIN 1 cacgacctgc ggcggcgggc gcaacaaagc tggggaagcg gccccgtgtt ttgaggaatc 61 attgcctcgg ggtctctccg agtctgcccc ggactgagat ttaggcgggc tgctgcagca 121 ggttgcggcg acggcaagca ctggggcttg gcgggggtca aaccaccgcc tgccccgcgg 181 atgctgtgtt gggtgttggg ttggcggtcg ttgtacgtac ctgctcagct ctgctcggct 241 gggcttctgg ttattgccag tagccggggc tgctggctga caggattcgc acacgactcg 301 gttctgcaag gaaccccgcc ccacctggct ggtgcagcga ctccgaggcc tcgccttgcc 361 tccagctgca gggttcaacg cggcatctcg ggtattgcag gtcttgcaaa caaaaagtct 421 agaagtgtct ttgtccgctc gccgtcgggg cgcatccttc ttgccggttg gccagccgcc 481 ggcgcgacgt gatcgcagtc acgaccccac tggcgatggg cccgagaccc aaaagccccg 541 aatgagcgac tggagctccc cgcttgagca tcgtcagttc gacgacaagg cgcatcgcat 601 gatgtcagcc ttgcaggcca aagttggcca cgcgccgagc atttgccatg cccagacttg 661 catcacaacg ccacattcac aacatcgata ctgacaatgt gcgtctacag acactcaagg 721 agaaggagta c

18／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS30 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence

GenBank: MK446257.1 FASTA Graphics PopSet Go to: LOCUS MK446257 507 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS30 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. ACCESSION MK446257 VERSION MK446257.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 507) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 507) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..507 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS30" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" ORIGIN 1 attactgagt ttacgctcta caaccctttg tgaacatacc tataactgtt gcttcggcgg 61 gtagggtccc cgtgaccctc ccggcctccc gcccccgggc gggtcggcgc ccgccggagg 121 ataaccaaac tctgatttaa cgacgtttct tctgagtggt acaagcaaat aatcaaaact 181 tttaacaacg gatctcttgg ttctggcatc gatgaagaac gcagcgaaat gcgataagta 241 atgtgaattg cagaattcag tgaatcatcg aatctttgaa cgcacattgc gcccgccagc 301 attctggcgg gcatgcctgt tcgagcgtca tttcaaccct caagctctgc ttggtgttgg 361 ggccctacag ctgatgtagg ccctcaaagg tagtggcgga ccctcccgga gcctcctttg 421 cgtagtaact ttacgtctcg cactgggatc cggagggact cttgccgtaa aaccccccaa 481 ttttccaaag gttgacctcg gatcagg //

19／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS35 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence

GenBank: MK446258.1 FASTA Graphics PopSet Go to: LOCUS MK446258 507 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS35 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence. ACCESSION MK446258 VERSION MK446258.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 507) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 507) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..507 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS35" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" ORIGIN 1 attactgagt ttacgctcta caaccctttg tgaacatacc tataactgtt gcttcggcgg 61 gtagggtctc cgtgaccctc ccggcctccc gcccccgggc gggtcggcgc ccgccggagg 121 ataaccaaac tctgatttaa cgacgtttct tctgagtggt acaagcaaat aatcaaaact 181 tttaacaacg gatctcttgg ttctggcatc gatgaagaac gcagcgaaat gcgataagta 241 atgtgaattg cagaattcag tgaatcatcg aatctttgaa cgcacattgc gcccgccagc 301 attctggcgg gcatgcctgt tcgagcgtca tttcaaccct caagctctgc ttggtgttgg 361 ggccctacag ctgatgtagg ccctcaaagg tagtggcgga ccctcccgga gcctcctttg 421 cgtagtaact ttacgtctcg cactgggatc cggagggact cttgccgtaa aaccccccaa 481 ttttccaaag gttgacctcg gatcagg //

20／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS30 beta-tubulin-2 (tub2) gene, partial cds

GenBank: MK446259.1 FASTA Graphics PopSet Go to: LOCUS MK446259 644 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS30 beta-tubulin-2 (tub2) gene, partial cds. ACCESSION MK446259 VERSION MK446259.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 644) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 644) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..644 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS30" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <135..>644 /gene="tub2" mRNA join(<135..158,217..242,314..355,411..465,537..>644) /gene="tub2" /product="beta-tubulin-2" CDS join(<135..158,217..242,314..355,411..465,537..>644) /gene="tub2" /codon_start=1 /product="beta-tubulin-2" /protein_id="QCW06517.1" /translation="VHLQTGQCGNQIGAAFWQNISGEHGLDSNGVYNGTSELQLERMS VYFNEASGNKYVPRAVLVDLEPGTMDAVRAGPFGQLFRPDN" ORIGIN 1 atccgccttg cccctgagcg taccccgccg acatttttac ccgacttcta tgctcaacaa 61 acccgcgacg cctgtcaatc atcgacgtcc aactctggaa tgttttgctg actgctgctt 121 ttttttgtct acaggttcac ctccagaccg gccagtgcgt aagtcttccc aagccaaatc 181 caaccgcctg attgcggggc taacctcctt gtacagggta accagattgg tgctgccttc 241 tggtacgtga cgagaccgcc gacgatccgg caatatatac ttgcgaggac ggcagatgtt 301 gacgatagag taggcaaaac atttctggcg agcacggcct cgacagcaat ggagtgtatg 361 tcatgcccct tatctggcca cattggtggt tgaccgctaa actcgaacag ctacaacggc 421 acctctgagc tccagctcga gcgcatgagc gtctacttca acgaagtttg ttaccttata 481 gcccccagag tgcaagataa acatattgac gagtactgac cttcgctcct acccaggctt 541 ccggcaacaa gtacgtgccc cgtgccgtcc tcgtcgattt ggagcccggt accatggacg 601 ccgtccgtgc cggtcctttc ggccagctct tccgccccga caac

21／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN Colletotrichum siamense isolate RS35 beta-tubulin-2 (tub2) gene, partial cds

GenBank: MK446260.1 FASTA Graphics PopSet Go to: LOCUS MK446260 644 bp DNA linear PLN 04-JUN-2019 DEFINITION Colletotrichum siamense isolate RS35 beta-tubulin-2 (tub2) gene, partial cds. ACCESSION MK446260 VERSION MK446260.1 KEYWORDS . SOURCE Colletotrichum siamense ORGANISM Colletotrichum siamense Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum. REFERENCE 1 (bases 1 to 644) AUTHORS He,L. TITLE First report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China JOURNAL Unpublished REFERENCE 2 (bases 1 to 644) AUTHORS He,L. TITLE Direct Submission JOURNAL Submitted (23-JAN-2019) Forestry college, Central South University of Forestry and Technology, 498 Shaoshan South Road, Changsha, Hunan 410004, P.R. China COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..644 /organism="Colletotrichum siamense" /mol_type="genomic DNA" /isolate="RS35" /isolation_source="leaf spot" /host="Rosa chinensis" /db_xref="taxon:690259" /country="China" /collection_date="2018-04-26" gene <135..>644 /gene="tub2" mRNA join(<135..158,217..242,314..355,411..465,537..>644) /gene="tub2" /product="beta-tubulin-2" CDS join(<135..158,217..242,314..355,411..465,537..>644) /gene="tub2" /codon_start=1 /product="beta-tubulin-2" /protein_id="QCW06518.1" /translation="VHLQTGQCGNQIGAAFWQNISGEHGLDSNGVYNGTSELQLERMS VYFNEASGNKYVPRAVLVDLEPGTMDAVRAGPFGQLFRPDN" ORIGIN 1 atccgccttg cccctgagcg taccccgccg acatttttac ccgacttcta tgctcaacaa 61 acccgcgacg cctgtcaatc atcgacgtcc aactctggaa tgttttgctg actgctgctt 121 ttttttgtct acaggttcac ctccagaccg gccagtgcgt aagtcttccc aagccaaatc 181 caaccgcctg attgcggggc taacctcctt gtacagggta accagattgg tgctgccttc 241 tggtacgtga cgagaccgcc gacgatccgg caatatatac ttgcgaggac ggcagatgtt 301 gacgatagag taggcaaaac atttctggcg agcacggcct cgacagcaat ggagtgtatg 361 tcatgcccct tatctggcca cattggtggt tgaccgctaa actcgaacag ctacaacggc 421 acctctgagc tccagctcga gcgcatgagc gtctacttca acgaagtttg ttaccttata 481 gcccccagag tgcaagataa acatattgac gagtactgac cttcgctcct acccaggctt 541 ccggcaacaa gtacgtgccc cgtgccgtcc tcgtcgattt ggagcccggt accatggacg 601 ccgtccgtgc cggtcctttc ggccagctct tccgccccga caac

22／22 https://apsjournals.apsnet.org/doi/10.1094/PDIS-11-18-1966-PDN