DOCSLIB.ORG

Beneficial Effects of Adiponectin on Periodontal Ligament Cells Under Normal and Regenerative Conditions

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Beneficial Effects of Adiponectin on Periodontal Ligament Cells Under Normal and Regenerative Conditions Hindawi Publishing Corporation Journal of Diabetes Research Volume 2014, Article ID 796565, 11 pages http://dx.doi.org/10.1155/2014/796565 Research Article Beneficial Effects of Adiponectin on Periodontal Ligament Cells under Normal and Regenerative Conditions Marjan Nokhbehsaim,1,2 Sema Keser,2 Andressa Vilas Boas Nogueira,2,3 Joni Augusto Cirelli,3 Søren Jepsen,2,4 Andreas Jäger,2,5 Sigrun Eick,6 and James Deschner1,2 1 Experimental Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, Germany 2 Clinical Research Unit 208, University of Bonn, 53111 Bonn, Germany 3 Department of Diagnosis and Surgery, School of Dentistry, UNESP, 14801-903 Araraquara, SP, Brazil 4 Department of Periodontology, Operative and Preventive Dentistry, University of Bonn, 53111 Bonn, Germany 5 Department of Orthodontics, University of Bonn, 53111 Bonn, Germany 6 Department of Periodontology, Laboratory of Oral Microbiology, University of Bern, 3010 Bern, Switzerland Correspondence should be addressed to James Deschner; [email protected] Received 16 April 2014; Revised 24 June 2014; Accepted 25 June 2014; Published 13 July 2014 Academic Editor: Ronald G. Tilton Copyright © 2014 Marjan Nokhbehsaim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Type 2 diabetes and obesity are increasing worldwide and linked to periodontitis, a chronic disease which is characterized by the irreversible destruction of the tooth-supporting tissues, that is, periodontium. The mechanisms underlying the association of diabetes mellitus and obesity with periodontal destruction and compromised periodontal healing are not well understood, but decreased plasma levels of adiponectin, as found in diabetic and obese individuals, might be a critical mechanistic link. The aim of this in vitro study was to examine the effects of adiponectin on periodontal ligament (PDL) cells under normal and regenerative conditions, and to study the regulation of adiponectin and its receptors in these cells. Adiponectin stimulated significantly the expression of growth factors and extracellular matrix, proliferation, and in vitro wound healing, reduced significantly the constitutive tumor necrosis factor- expression, and caused a significant upregulation of its own expression. The beneficial actions of enamel matrix derivative on a number of PDL cell functions critical for periodontal regeneration were partially enhanced by adiponectin. The periodontopathogen Porphyromonas gingivalis inhibited the adiponectin expression and stimulated the expression of its receptors. In conclusion, reduced levels of adiponectin, as found in type 2 diabetes and obesity, may compromise periodontal health and healing. 1. Introduction ligament (PDL), root cementum, and alveolar bone. Microor- ganisms, such as Fusobacterium nucleatum, Aggregatibacter The prevalence of diabetes mellitus and its associated comor- actinomycetemcomitans, Porphyromonas gingivalis,andTre- bidities is increasing worldwide [1]. Obesity is considered ponema denticola, in the subgingival plaque on the tooth a major risk factor for type 2 diabetes and has also risen surfaces are essential for the initiation and progression of substantially throughout the globe over the past decades periodontitis [8, 9]. Several cofactors, such as smoking, [2, 3]. A number of meta-analyses have demonstrated that can increase the risk for periodontitis [10]. The periodon- diabetes mellitus and obesity are linked to periodontitis, a topathogenic microorganisms provoke an inflammatory host chronic disease characterized by the irreversible destruction response, which involves inflammatory mediators, such as of the tooth-supporting tissues, that is, periodontium [4– interleukin- (IL-) 1,IL-6,IL-8,tumornecrosisfactor(TNF) 7]. The periodontium consists of the gingiva, periodontal , and cyclooxygenase (COX) 2 in the periodontal tissues. If 2 Journal of Diabetes Research the periodontal inflammation is exaggerated and sustained, increased and associated with proinflammatory effects42 [ ]. matrix degradation, bone resorption, periodontal pocket Asshowninourpreviousstudies,proinflammatorysignals formation, and even tooth loss can follow [10, 11]. can reduce the regenerative capacity of periodontal cells and Periodontitis can be successfully treated by either non- therefore inhibit regenerative healing [43–46]. So far, little surgical or surgical approaches, sometimes combined with is known about the actions of adiponectin on periodontal antibiotics. The main goal of periodontal therapy is to arrest homeostasis and regeneration. The aim of this in vitro study inflammation and periodontal tissue destruction by reducing was to examine the effects of adiponectin on periodontal cells or even eliminating the periodontopathogenic microorgan- under normal and regenerative conditions and to study the isms in the periodontal pockets [12]. In order to promote regulation of adiponectin and its receptors in these cells. regenerative healing, bioactive molecules, such as enamel matrix derivative (EMD), are applied during periodontal surgery [13–15]. EMD stimulates periodontal cells to produce 2. Materials and Methods growth factors, such as transforming growth factor (TGF) 2.1. Culture and Stimulation of Cells. PDL cells were obtained 1 and vascular endothelial growth factor (VEGF), matrix from 18 periodontally healthy donors, who underwent tooth molecules, such as periostin (POSTN), and osteogenesis- extractions for orthodontic reasons. Written informed con- related factors, such as runt-related transcription factor sent and approval of the Ethics Committee of the University (RUNX) 2. Moreover, EMD has been shown to accelerate the of Bonn were obtained. PDL cells (passages 3–5) were seeded in vitro wound healing [16, 17]. The beneficial effects of EMD on culture plates (50,000 cells/well) and grown to 80% con- on periodontal regeneration are accomplished, at least in part, fluence in Dulbecco’s minimal essential medium (DMEM, by bone morphogenetic protein (BMP) and TGF,which Invitrogen, Karlsruhe, Germany) supplemented with 10% trigger SMAD (sma- and mad-related protein) and non- fetal bovine serum (FBS, Invitrogen), 100 units penicillin, and SMAD signaling cascades [18–22]. Interestingly, microbial, ∘ 100 g/mL streptomycin (Invitrogen) at 37 Cinahumidified inflammatory, and biomechanical signals can interfere with atmosphere of 5% CO2. Before the experiments, the FBS the regeneration-promotive effects of EMD on periodontal concentration was reduced to 1%, and medium was changed cells, which highlights the critical role of the cell microen- every other day. vironment for regenerative healing [23–25]. In order study the effects of adiponectin on PDL cells, Interestingly, diabetes mellitus and obesity are associated physiological concentrations of adiponectin (0.3, 1, and not only with the initiation and progression of periodontitis, 3 g/mL; HMW oligomers, R&D Systems, Minneapolis, MN, but also with compromised healing after periodontal therapy USA) were added to the cells [47]. Cells were also treated [26–28]. The mechanisms underlying the associations of with EMD (Emdogain, Straumann, Freiburg, Germany) diabetes mellitus and obesity with periodontitis and com- used to mimic regenerative conditions at a concentration promised periodontal healing are not well understood so of 100 g/mL. To simulate inflammatory conditions, cells far. However, altered plasma levels of adipokines, as found were incubated with IL-1 (1 ng/mL; Calbiochem, San Diego, in diabetic and obese individuals, might be a critical mech- CA, USA). An infectious environment was mimicked by anistic link in these associations. Adipokines are bioactive treating cells with the inactivated oral periodontopathogens molecules, which are produced in the adipose tissue. In F. nucleatum ATCC 25586 (optical density: 0.1), A. actino- diabetes mellitus and obesity, plasma levels of proinflam- mycetemcomitans ATCC 43718 (OD: 0.1), P. g ing iv ali s ATCC matory adipokines, such as visfatin, leptin, and resistin, are 33277 (OD: 0.025–0.2), or T. denticola ATCC 35405 (OD: 0.1). increased, whereas the levels of adiponectin, which is an Bacteria were suspended in PBS (OD660 nm = 1, equivalent anti-inflammatory adipokine, are decreased. The imbalance 9 to 1.2 × 10 bacterial cells/mL) and subjected two times to between pro- and anti-inflammatory adipokines contributes ultrasonication (160 W for 15 min) resulting in a complete to the low-grad inflammatory state, as observed in diabetes killing. In order to ensure that results were comparable, EMD, mellitus and obesity [29–32]. IL-1, and the periodontopathogens were applied at the same Adiponectin is mainly synthesized by adipocytes. By physiological doses as in our previous experiments [43–46, binding to its receptors (AdipoR1 and AdipoR2), adiponectin 48–50]. activates the adenosine monophosphate-activated protein In order to unravel the intracellular signaling involved kinase (AMPK) and other pathways [33–35]. Like other in possible actions of adiponectin, PDL cells were also adipokines, adiponectin has both metabolic and immune preincubated with a specific inhibitor against the AMPK functions. Adiponectin enhances fatty acid oxidation, insulin signaling pathway (dorsomorphin; 5 M; Calbiochem, San sensitivity, and glucose uptake and inhibits the hepatic glu- Diego, CA, USA) 45 min before the experiments.
Load more

Recommended publications
  • Assessment of Efficacy of Chlorhexidine Chip As an Adjunct to Scaling and Root Planning Using N-Benzoyl- DL-Arginine-2-Naphthylamide Test Kit
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Asian Pacific Journal of Health Sciences e-ISSN: 2349-0659 p-ISSN: 2350-0964 ORGINAL ARTICLE doi: 10.21276/apjhs.2018.5.2.21 Assessment of efficacy of chlorhexidine chip as an adjunct to scaling and root planning using N-benzoyl- DL-arginine-2-naphthylamide test kit Malvika Singh*, Rajan Gupta, Parveen Dahiya, Mukesh Kumar, Rohit Bhardwaj Department of Periodontics, Himachal Institute of Dental Sciences, Paonta Sahib, Sirmaur, Himachal Pradesh, India ABSTRACT Background: With increasing advances in the field of medicine, diagnosing a disease has been an easy task and periodontitis is no exception to this. N-benzoyl-DL-arginine-2-naphthylamide (BANA) test is a modern chair-side paraclinical method designed to detect the presence of one or more anaerobic bacteria commonly associated with periodontal disease, namely Treponema denticola, Porphyromonas gingivalis, and Tannerella forsythia in subgingival plaque samples taken from periodontally diseased teeth. Aim: The aim of the study was to assess the efficacy of chlorhexidine chip as an adjunct to scaling and root planning (SRP), using BANA Test Kit. Materials and Methods: A total of 20 chronic periodontitis patients (aged 35–55 years) having pocket depth of ≥5 mm in molar teeth were selected and randomly divided into following treatment groups: Group I: SRP and Group II: SRP along with chlorhexidine chip. The clinical and microbial parameters were recorded at baseline and 1 and 3 months post-treatment. BANA chairside test was used for estimation of specific microbiota. Statistical analysis used: Mann–Whitney test, Wilcoxon signed test, t-test, Pearson’s Chi-square test, and variability test were used.
    [Show full text]
  • Probiotic Alternative to Chlorhexidine in Periodontal Therapy: Evaluation of Clinical and Microbiological Parameters
    microorganisms Article Probiotic Alternative to Chlorhexidine in Periodontal Therapy: Evaluation of Clinical and Microbiological Parameters Andrea Butera , Simone Gallo * , Carolina Maiorani, Domenico Molino, Alessandro Chiesa, Camilla Preda, Francesca Esposito and Andrea Scribante * Section of Dentistry–Department of Clinical, Surgical, Diagnostic and Paediatric Sciences, University of Pavia, 27100 Pavia, Italy; [email protected] (A.B.); [email protected] (C.M.); [email protected] (D.M.); [email protected] (A.C.); [email protected] (C.P.); [email protected] (F.E.) * Correspondence: [email protected] (S.G.); [email protected] (A.S.) Abstract: Periodontitis consists of a progressive destruction of tooth-supporting tissues. Considering that probiotics are being proposed as a support to the gold standard treatment Scaling-and-Root- Planing (SRP), this study aims to assess two new formulations (toothpaste and chewing-gum). 60 patients were randomly assigned to three domiciliary hygiene treatments: Group 1 (SRP + chlorhexidine-based toothpaste) (control), Group 2 (SRP + probiotics-based toothpaste) and Group 3 (SRP + probiotics-based toothpaste + probiotics-based chewing-gum). At baseline (T0) and after 3 and 6 months (T1–T2), periodontal clinical parameters were recorded, along with microbiological ones by means of a commercial kit. As to the former, no significant differences were shown at T1 or T2, neither in controls for any index, nor in the experimental
    [Show full text]
  • Exam # ___DAPE 731, Periodon
    Exam 1-A NAME: _ _____ KEY A & B________________ Seat #: __________________________________ Exam # _________ DAPE 731, Periodontology Year III Dr. Elio Reyes, D.D.S., M.S.D. Dr. Dwight E. McLeod, D.D.S., M.S. October 13, 2009 USE Pencil on Separate Answer Sheet 50 questions multiple choice & true/false – 2 pts each 100 points 3 bonus questions fill in the blank – 1 point each. Total possible points 103 Which periodontal procedure has the specific goal of removing the epithelial lining of the periodontal pocket? A. Periodontal prophylaxis B. Gingival curettage C. Subgingival scaling and root planing D. Gingivoplasty E. None of the above Which one of the following procedures is considered a gingival curettage procedure? A. Gingivectomy B. Subgingival scaling and root planing C. Electrocautery procedure D. Excisional new attachment procedure E. The inverse bevel technique A positive Nikolsky’s sign determines the difference between these conditions: A. Cicatrical pemphigoid / Mucous membrane pemphigoid. B. Mucous membrane pemphigoid/ Pemphigus vulgaris. C. Pemphigus vulgaris/ Stomatitis medicamentosa. D. Stomatitis medicamentosa/ Cicatrical pemphigoid. E. None of the above. Which one of the following conditions is not classified into the “Periodontitis as a manifestation of systemic diseases” category? A. Hematological disorders B. Genetic disorders C. Diabetes mellitus D. A and B E. None of the above In distinguishing “Necrotizing Ulcerative Periodontitis” from “Periodontitis associated with Systemic Diseases”, which of the following factors would best assist you to differentiate? A. The amount of subgingival calculus present. B. Level of the mucogingival junction. C. Presence of pseudomembranous sloughed layer. D. Bleeding upon probing. Identify the tissues that comprise the pocket wall: 1.
    [Show full text]
  • Instant Update- Getting up to Speed in Periodontics for 2019 Pennsylvania Dental Association Gettysburg Meeting April 6, 2019 F
    Instant Update- Getting Up To Speed in Periodontics for 2019 Pennsylvania Dental Association Gettysburg Meeting April 6, 2019 Francis G. Serio, DMD, MS, MBA Diplomate, American Board of Periodontology Staff Dentist, Greene County Health Care, Inc. Course Synopsis Some things change and some things remain the same. The bedrocks of periodontal therapy are time-tested but new approaches to some of these therapies are providing better outcomes for patients. In addition, advances in the science of periodontics have led to both a better understanding of the disease processes and a new classification system for the periodontal diseases and conditions. In addition, as implant dentistry continues to solidify its position, complications are becoming more commonplace. This course will focus on four main areas: The changes in science that have led to the new classification of the periodontal diseases and conditions. Current understanding of the perio-systemic connection. The “semi-surgical” approach to periodontal therapy. Peri-implant mucositis and peri-implantitis and what to do about it. At the end of this presentation, each participant will be able to: Identify the differences between the 1999 and 2017 disease classification systems. Identify key factors and systemic diseases that have a strong association with the periodontal diseases. Develop a “semi-surgical” treatment plan for a patient with periodontitis. Understand the key factors that contribute to peri-implant disease and possible therapeutic approaches. Periodontitis is a disease of the non-mineralized and mineralized connective tissues- What causes and contributes to its breakdown? Bacterial infections vs. Inflammation 1 Statistical vs. Clinical Significance Clinical significance- Jacobson, et al.
    [Show full text]
  • Clinical Sheet
    Dental Surgery Clinical Sheet PERIODONTAL REGENERATION ON TWO TEETH AFFECTED BY A SEVERE PERIODONTAL DISEASE Use of an equine origin granular graft in combination with an amelogenin gel. In subjects most at risk, the inflammatory response to bacterial colonization of gingival pockets may give rise to periodontitis, a destructive process that leads to the loss of some of the bone and connective tissue, as well as to apical migration of junctional epithelium. Starting from typical symptoms of gingivitis, characterized by redness, swelling and bleeding tendency, over time one observes a significant change in the biofilm species, where A. actinomycetemcomitans start becoming predominant, as well as red complex bacteria and, to a lesser extent, those of the orange complex. The progress of periodontitis is not constant over time and may vary from person to person, however in its aggressive forms it often eventually leads to the loss of the affected members. Causal therapy eliminates the microbial biofilm and the inflammatory condition caused by it, Francesco Bellucci Private practitioner but the tissue defects will not be able to repair spontaneously. Hence the clinical need to have Avellino, Italy recourse to regeneration procedures that include the alveolar process, to achieve preservation [email protected] of the members originally involved in the pathology for the longest time possible. Within this framework, using a graft that may be remodeled by osteoclasts and that is very easy to handle may be a significant aid in achieving long-term clinical success. The use of amelogenin-based gels may be just as useful due to their action on the periodontal ligament.
    [Show full text]
  • Principles of Periodontology Andrew R
    Marquette University e-Publications@Marquette School of Dentistry Faculty Research and Dentistry, School of Publications 2-1-2013 Principles of Periodontology Andrew R. Dentino Marquette University, [email protected] Seokwoo Lee Jason Mailhot Arthur F. Hefti Marquette University, [email protected] Accepted version. Periodontology 2000, Vol. 61, No. 1 (February 2013): 16-53. DOI. © 1999-2018 John Wiley & Sons, Inc. Used with permission. Marquette University e-Publications@Marquette Dentistry Faculty Research and Publications/School of Dentistry This paper is NOT THE PUBLISHED VERSION; but the author’s final, peer-reviewed manuscript. The published version may be accessed by following the link in the citation below. Periodontology 2000, Vol. 61, No. 1 (2013): 16-53. DOI. This article is © Wiley and permission has been granted for this version to appear in e-Publications@Marquette. Wiley does not grant permission for this article to be further copied/distributed or hosted elsewhere without the express permission from Wiley. Table of Contents Abstract ......................................................................................................................................................... 3 History ........................................................................................................................................................... 5 Early Observations .................................................................................................................................... 5 From
    [Show full text]
  • Periodontics – Solid Foundations for Saving Teeth
    Periodontics – Solid Foundations for Saving Teeth Andre Bendyk Research Disease Initiation and Progression 1)Bacteria 2)Host 3)Environment al Influences Periodontopathogens P.gingivalis A. actinomycetemcomitans P. intermedia T. forsythensis T. denticola Plaque Biofilms • Spatial and Functional organisation between species • Mature with time Microbial Complexes in Subgingival Plaque Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL J Clin Periodontol. 1998 Feb;25(2):134-44. Cugini et al, Journal of Periodontology 2000 Red Complex A range of studies have demonstrated that members of the red complex are: 1) More common in deep than shallow sites 2) Isolated more frequently from pockets thought to have undergone recent destruction compared to non progressing sites 3) Found very frequently in significant quantities in cases of periodontitis which prove refractory to treatment Red Complex Holt and Ebersole (05) suggest that these red complex organisms have many host activating properties which act together to exert ‘pathogenic synergism’ Pathogenesis 1)Bacteria 2)Host 3)Environment al Influences Host In human periodontitis, the vast majority of periodontal tissue destruction occurs as the result of a dysregulated immunoinflammatory response to periodontopathic bacteria and their virulence factors – rather than as a direct action of bacterial products themselves Host While inflammation is a critical protective mechanism, an aberrant inflammatory response associated with cytokines such as IL- 1, IL-6, IL-8, TNFα and arachidonic acid metabolites (eg PGE2) is seen in periodontitis patients. This promotes the production and release of matrix metalloproteinases by inlammatory and tissue cells, and also stimulates osteoclast activity. Host Host Genetics Michalowicz et al (2000) looked at a range of clinical measures in monozygous and dizygous twins.
    [Show full text]
  • Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults
    fmicb-07-00840 May 31, 2016 Time: 12:58 # 1 ORIGINAL RESEARCH published: 02 June 2016 doi: 10.3389/fmicb.2016.00840 Quantitative Molecular Detection of 19 Major Pathogens in the Interdental Biofilm of Periodontally Healthy Young Adults Florence Carrouel1†, Stéphane Viennot2†, Julie Santamaria3, Philippe Veber4 and Denis Bourgeois2* 1 Institute of Functional Genomics of Lyon, UMR CNRS 5242, Ecole Normale Supérieure de Lyon, University Lyon 1, Lyon, France, 2 Laboratory “Health, Individual, Society” EA4129, University Lyon 1, Lyon, France, 3 Department of Prevention and Public Health, Faculty of Dentistry, University Lyon 1, Lyon, France, 4 Laboratory “Biométrie et Biologie Évolutive”, UMR CNRS 5558 – LBBE, University Lyon 1, Villeurbanne, France In oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. The interdental spaces are Edited by: Yuji Morita, the source of many hypotheses regarding their potential associations with and/or Aichi Gakuin University, Japan causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative Reviewed by: disease, and depression. This PCR study is the first to describe the interdental Kah Yan How, University of Malaya, Malaysia microbiota in healthy adults aged 18–35 years-old with reference to the Socransky Aurea Simón-Soro, complexes. The complexes tended to reflect microbial succession events in developing FISABIO Foundation, Spain dental biofilms. Early colonizers included members of the yellow, green, and Guliz N. Guncu, Hacettepe University, Turkey purple complexes. The orange complex bacteria generally appear after the early *Correspondence: colonizers and include many putative periodontal pathogens, such as Fusobacterium Denis Bourgeois nucleatum.
    [Show full text]
  • Prevotella Intermedia
    The principles of identification of oral anaerobic pathogens Dr. Edit Urbán © by author Department of Clinical Microbiology, Faculty of Medicine ESCMID Online University of Lecture Szeged, Hungary Library Oral Microbiological Ecology Portrait of Antonie van Leeuwenhoek (1632–1723) by Jan Verkolje Leeuwenhook in 1683-realized, that the film accumulated on the surface of the teeth contained diverse structural elements: bacteria Several hundred of different© bacteria,by author fungi and protozoans can live in the oral cavity When these organisms adhere to some surface they form an organizedESCMID mass called Online dental plaque Lecture or biofilm Library © by author ESCMID Online Lecture Library Gram-negative anaerobes Non-motile rods: Motile rods: Bacteriodaceae Selenomonas Prevotella Wolinella/Campylobacter Porphyromonas Treponema Bacteroides Mitsuokella Cocci: Veillonella Fusobacterium Leptotrichia © byCapnophyles: author Haemophilus A. actinomycetemcomitans ESCMID Online C. hominis, Lecture Eikenella Library Capnocytophaga Gram-positive anaerobes Rods: Cocci: Actinomyces Stomatococcus Propionibacterium Gemella Lactobacillus Peptostreptococcus Bifidobacterium Eubacterium Clostridium © by author Facultative: Streptococcus Rothia dentocariosa Micrococcus ESCMIDCorynebacterium Online LectureStaphylococcus Library © by author ESCMID Online Lecture Library Microbiology of periodontal disease The periodontium consist of gingiva, periodontial ligament, root cementerum and alveolar bone Bacteria cause virtually all forms of inflammatory
    [Show full text]
  • Research Study Periodontal Aspects in Orthodontics
    Research study Periodontal aspects in orthodontics Periodontology Abstract Results Introduction Porphyromonas g i n g i v a l i s , annerella forsythia,Treponema denticola, Conclusion Porphyromonas gingivalis, Tannerella forsythiaTreponema denticola manuscript. nal fi Introduction Advantages of OT given to periodontal patients Alignment and levelling Materials and methods the and approved as read well as the manuscript, on of Tooth crown clinical extension none declared. ict of interests: fl rules of disclosure. (AME) ethical Ethics Medical on for Progressive periodontal disease with horizontal bone reabsorption none declared. Con ng interests: Lauritano D, Caccianiga G. Compe prepara design, and on, the concept to authors contributed All the Associa by abide authors All Research study Protesic and implanting molar uprighting et al. et al. Porphyromonas gin- nal manuscript. givalis (P. gingivalis), Prevotella fi intermedia (P. intermedia)/P. nigres- Periodontal remodelling during OT cens, T. forsythia Fusobacterium
    [Show full text]
  • An Exploratory Statistical Method for Finding Interactions in a Large Dataset with an Application Toward Periodontal Diseases
    University of Kentucky UKnowledge Theses and Dissertations--Epidemiology and Biostatistics College of Public Health 2017 An Exploratory Statistical Method For Finding Interactions In A Large Dataset With An Application Toward Periodontal Diseases Joshua Lambert University of Kentucky, [email protected] Digital Object Identifier: https://doi.org/10.13023/ETD.2017.448 Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Lambert, Joshua, "An Exploratory Statistical Method For Finding Interactions In A Large Dataset With An Application Toward Periodontal Diseases" (2017). Theses and Dissertations--Epidemiology and Biostatistics. 16. https://uknowledge.uky.edu/epb_etds/16 This Doctoral Dissertation is brought to you for free and open access by the College of Public Health at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Epidemiology and Biostatistics by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine) which will be submitted to UKnowledge as Additional File. I hereby grant to The University of Kentucky and its agents the irrevocable, non-exclusive, and royalty-free license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known.
    [Show full text]
  • Shift in the Subgingival Microbiome Following Scaling and Root Planing in Generalized Aggressive Periodontitis
    Accepted: 14 December 2017 DOI: 10.1111/jcpe.12862 EPIDEMIOLOGY (COHORT STUDY OR CASE–CONTROL STUDY) Shift in the subgingival microbiome following scaling and root planing in generalized aggressive periodontitis Guojing Liu1 | Qingxian Luan1 | Feng Chen2 | Zhibin Chen1 | Qian Zhang2 | Xiaoqian Yu1 1Department of Periodontology, Peking University School and Hospital of Abstract Stomatology, Beijing, China Aim: To investigate the shift in the subgingival microbiota under scaling and root plan- 2 Central Laboratory, Peking University School ing (SRP) in patients with generalized aggressive periodontitis (GAgP). and Hospital of Stomatology, Beijing, China Materials and Methods: After undergoing supragingival scaling, 12 individuals with Correspondence GAgP were enrolled in this longitudinal study. Full- mouth SRP was accomplished in Xiaoqian Yu, Department of Periodontology, Peking University School and Hospital of 3 weeks and re- evaluated 6 weeks later. Pooled subgingival samples (posterior- mesial, Stomatology, Beijing, China. posterior- buccal, anterior- mesial, and anterior- buccal) were obtained from each pa- Email: [email protected] tient before SRP (pre- treatment group) and at the time of re- evaluation (post- treatment Funding information This work was supported by the National group). 16S rRNA PCR products were generated and sequenced after DNA isolation. Natural Science Foundation of China Results: Under SRP, the diversity of the subgingival community was consistent, (81470740). whereas genus- level biomarkers transformed from Porphyromonas, Treponema, and Fretibacterium to Actinomyces, Streptococcus, and Haemophilus. In a network analysis, pathogen- related and non- pathogen- related components were identified in both the pre- and post- treatment groups; the pathogen component was dramatically aug- mented, while the non- pathogen component shrank after treatment.
    [Show full text]