PPT1 Antibody Cat. No.: 61-421

PPT1 Antibody

Formalin-fixed and paraffin-embedded human lung Western blot analysis of PPT1 using rabbit polyclonal PPT1 carcinoma tissue reacted with PPT1 antibody , which was Antibody (A284) using 293 cell lysates (2 ug/lane) either peroxidase-conjugated to the secondary antibody, nontransfected (Lane 1) or transiently transfected (Lane 2) followed by DAB staining. with the PPT1 .

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Monkey

This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 269-300 amino acids from the C-terminal region of human PPT1.

TESTED APPLICATIONS: IHC-P, WB

For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:50~100

September 29, 2021 1 https://www.prosci-inc.com/ppt1-antibody-61-421.html PREDICTED MOLECULAR 34 kDa WEIGHT:

Properties

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by PURIFICATION: dialysis

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: PPT1

ALTERNATE NAMES: Palmitoyl-protein 1, PPT-1, Palmitoyl-protein 1, PPT1, PPT

ACCESSION NO.: P50897

PROTEIN GI NO.: 1709747

GENE ID: 5538

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 29, 2021 2 https://www.prosci-inc.com/ppt1-antibody-61-421.html Palmitoyl-protein thioesterase-1 (PPT1) is a lysosomal hydrolase that removes long-chain fatty acyl groups from modified cysteine residues in proteins. Mutations in PPT1 have been found to cause the infantile form of neuronal ceroid lipofuscinosis (INCL), and an animal model has been developed.1 The deduced PPT2 protein contains 302 amino acids, including a 27-amino acid leader peptide, a sequence motif characteristic of many and lipases, and 5 potential N-linked glycosylation sites.2 PPT2 shares 18% amino acid identity with PPT1. Northern blot analysis detected a predominant 2.0-kb PPT2 BACKGROUND: transcript in the human tissues examined, with the highest expression in skeletal muscle; variable amounts of 2.8- and 7.0-kb transcripts were also observed. Recombinant PPT2, like PPT1, possesses thioesterase activity and localizes to the lysosome. Since PPT2 could not substitute for PPT1 in correcting the metabolic defect in INCL cells and was unable to remove palmitate groups from palmitoylated proteins that are routinely used as substrates for PPT1it has been postulated that PPT2 possesses a different substrate specificity than PPT1.

REFERENCES: 1) Calero, G., et al., J. Biol. Chem. 278(39):37957-37964 (2003).

2) Hofmann, S.L., et al., Curr. Mol. Med. 2(5):423-437 (2002).

3) Weimer, J.M., et al., Neuromolecular Med. 1(2):111-124 (2002).

4) Lu, J.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 93(19):10046-10050 (1996).

ANTIBODIES FOR RESEARCH USE ONLY.

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