RBMX Antibody (Center) Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap21472c

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RBMX Antibody (Center) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP21472c

Specification

RBMX Antibody (Center) - Product Information

Application WB,E Primary Accession P38159 Reactivity Human Host Rabbit Clonality polyclonal Isotype Rabbit Ig Calculated MW 42332

RBMX Antibody (Center) - Additional Information

Gene ID 27316

Other Names RNA-binding motif protein, X chromosome, Glycoprotein p43, Heterogeneous nuclear ribonucleoprotein G, hnRNP G, RNA-binding All lanes : Anti-RBMX Antibody (Center) at motif protein, X chromosome, N-terminally 1:2000 dilution Lane 1: Jurkat whole cell processed, RBMX, HNRPG, RBMXP1 lysates Lane 2: Hela whole cell lysates Lane 3: K562 whole cell lysates Lane 4: A431 Target/Specificity whole cell lysates Lysates/proteins at 20 µg This RBMX antibody is generated from a per lane. Secondary Goat Anti-Rabbit IgG, rabbit immunized with a KLH conjugated (H+L), Peroxidase conjugated at 1/10000 synthetic peptide between 222-252 amino dilution Predicted band size : 42 kDa acids from the Central region of human Blocking/Dilution buffer: 5% NFDM/TBST. RBMX.

Dilution WB~~1:2000

Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Precautions RBMX Antibody (Center) is for research use All lanes : Anti-RBMX Antibody (Center) at only and not for use in diagnostic or 1:2000 dilution Lane 1: A431 whole cell

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therapeutic procedures. lysates Lane 2: K562 whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat RBMX Antibody (Center) - Protein Information Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 42 kDa Blocking/Dilution buffer: Name RBMX 5% NFDM/TBST. Synonyms HNRPG, RBMXP1 RBMX Antibody (Center) - Background Function RNA-binding protein that plays several role in the regulation of pre- and RNA-binding protein that plays several role in post-transcriptional processes. Implicated in the regulation of pre- and post-transcriptional tissue- specific regulation of gene processes. Implicated in tissue-specific transcription and alternative splicing of regulation of gene transcription and alternative several pre-mRNAs. Binds to and stimulates splicing of several pre-mRNAs. Binds to and transcription from the tumor suppressor stimulates transcription from the tumor TXNIP gene promoter; may thus be involved suppressor TXNIP gene promoter; may thus be in tumor suppression. When associated with involved in tumor suppression. When SAFB, binds to and stimulates transcription associated with SAFB, binds to and stimulates from the SREBF1 promoter. Associates with transcription from the SREBF1 promoter. nascent mRNAs transcribed by RNA Associates with nascent mRNAs transcribed by polymerase II. Component of the RNA polymerase II. Component of the supraspliceosome complex that regulates supraspliceosome complex that regulates pre-mRNA alternative splice site selection. pre-mRNA alternative splice site selection. Can Can either activate or suppress exon either activate or suppress exon inclusion; acts inclusion; acts additively with TRA2B to additively with TRA2B to promote exon 7 promote exon 7 inclusion of the survival inclusion of the survival motor neuron SMN2. motor neuron SMN2. Represses the splicing Represses the splicing of MAPT/Tau exon 10. of MAPT/Tau exon 10. Binds preferentially Binds preferentially to single- stranded to single-stranded 5'-CC[A/C]-rich RNA 5'-CC[A/C]-rich RNA sequence motifs localized sequence motifs localized in a in a single-stranded conformation; probably single-stranded conformation; probably binds RNA as a homodimer. Binds binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking in the cytoplasmic TNFR1 trafficking pathways; pathways; promotes both the promotes both the IL-1- beta-mediated IL-1-beta-mediated inducible proteolytic inducible proteolytic cleavage of TNFR1 cleavage of TNFR1 ectodomains and the ectodomains and the release of TNFR1 release of TNFR1 exosome-like vesicles to exosome-like vesicles to the extracellular the extracellular compartment. compartment.

Cellular Location RBMX Antibody (Center) - References Nucleus. Note=Component of ribonucleosomes Localizes in numerous Soulard M.,et al.Nucleic Acids Res. small granules in the nucleus 21:4210-4217(1993). Venables J.P.,et al.Submitted (JUN-1998) to the Tissue Location EMBL/GenBank/DDBJ databases. Expressed strongly in oral keratinocytes, Lingenfelter P.A.,et al.Mamm. Genome but only weakly detected in oral squamous 12:538-545(2001). cell carcinomas (at protein level) Lin T.-Y.,et al.Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004). RBMX Antibody (Center) - Protocols

Provided below are standard protocols that you

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may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture RBMX Antibody (Center) - Citations RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers

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