Shc1 (Ab 427) Antibody Cat. No.: 79-412

Shc1 (Ab 427) Antibody

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Shc1 (Ab-427).Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Shc1 (Ab-427).

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Mouse, Rat

Shc1 (Ab-427) antibody was raised against a peptide sequence around aa.425~429 (P-S-Y- IMMUNOGEN: V-N) derived from Human Shc1.

TESTED APPLICATIONS: IF, IHC

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: APPLICATIONS: 1:100~1:200

SPECIFICITY: This antibody detects endogenous level of total Shc1 .

PREDICTED MOLECULAR 46, 52, 66 kDa WEIGHT:

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PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM BUFFER: NaCl, 0.02% sodium azide and 50% glycerol.

CONCENTRATION: 1 mg/mL

STORAGE CONDITIONS: Store antibody at -20˚C for up to one year.

Additional Info

OFFICIAL SYMBOL: SHC1

SHC, SHCA, GLUH1, GLUR1, SH2 domain protein C1, SHC, SHC-transforming protein 1, Src ALTERNATE NAMES: homology 2 domain-containing-transforming protein C1

ACCESSION NO.: NP_001123512.1

PROTEIN GI NO.: 194239664

GENE ID: 6464

Background and References

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal BACKGROUND: systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and . The expression of isoform p66Shc has been correlated with life span

1) Trampont P, et al. (2006) Mol Cell Biol; 26 (23): 9035-9044. Patrussi L, et al. (2005) REFERENCES: Oncogene; 24 (13): 2218-2228van der Geer P, et al. (1996) Curr Biol ; 6 (11): 1435-1444

ANTIBODIES FOR RESEARCH USE ONLY.

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