CLP Common Lymphoid Progenitor CMP Common Myeloid Progenitor B cell p4 B cell p15 CD4 T cell p5 CD4 T cell p16 HSC Hematopoietic Stem Cell CD8 T cell p6 CD8 T cell p17 ILC Innate Lymphoid Cell NK/ILC p7 NK/ILC p18 LTi Lymphoid Tissue inducer NKT/γδ T cell p8 NKT/γδ T cell p19 M1 classically activated Macrophage / Monocyte p9 Macrophage / Monocyte p20 M2 alternatively activated Dendritic cell p10 Dendritic cell p21 NK Natural Killer Granulocyte p11 Granulocyte p22 Tfh Follicular Helper T cell Megakaryocyte / Platelet p12 Megakaryocyte / Platelet p23 Th1 T Helper Type 1 cell Stem cell p13 Stem cell p24 Th2 T Helper Type 2 cell Other cell types p14 Other cell types p25 Treg Regulatory T cell

3

Human B cell markers

Pan markers Follicular Marginal zone Germinal center Plasma cell Memory CD19 Surface Surface Secreted Secreted Surface CD20 CD20 CD1d IgA IgA CD27 CD22 CD21 CD9 IgE IgE IgG CD70h CD22 CD21› IgG IgG TLR1, 2, 6, 7, 10 CD79α/β CD27 CD45R (B220) Intracellular/ Igκ/λ Surface Surface CD23h› CD81 transcription factor CD45R (B220) CD9 CD24 CD103 CBF1 CD81 CD19 low/neg CD40h CD180 (RP105) Pax5 CD20low/neg CD45R (B220) CD268 Intracellular/ Spi-B CD27 CD69h (BAFF receptor)› transcription factor CD31 CD80h CD307d (FcRL4) AID CD38 CD81 IgM Bach2 Bcl-6› CD81 CD86h Intracellular/ IRF8 CD138› CD137 (4-1BB)h transcription factor NF-kappaB CD184 (CXCR4) CD275 (B7-H2) EBF1 Pax5 CD252 (OX40 ligand) CD279 (PD-1) Notch2 CD269 (BCMA) CD360 (IL-2 receptor) Pax5 HLA-DR Intracellular/ IgDhigh› transcription factor IgMlow/int BLIMP1 IRF4 Intracellular/ XBP-1 transcription factor Bcl-6 EBF1 FoxO1 Ikaros Pax5

4 Human CD4 T cell markers

Pan markers Th1 Th2 Th17 Tfh Treg CD2 Secreted Secreted Secreted Secreted Secreted CD3 IFNγ Amphiregulin GM-CSF I L-21 I L-10 CD4 I L-2 I L- 4 I L-17A TGFβ CD5 Surface TNFα I L- 5 I L-17A F CD7 CD183 (CXCR3) Surface TNFβ (LTα) I L-13 I L-17F CD25h I L-21 CD185 (CXCR5)› CD25› CD27 Surface Surface I L-2 2 CD278 (ICOS)› CD39 CD28 CD94 CD184 (CXCR4) CD279 (PD-1)› CD73 CD45RA (naïve) CD119 (IFNγR1) CD194 (CCR4) Surface Intracellular/ CD101 CD45RO (memory) CD183 (CXCR3)› CD198 (CCR8) CD121a transcription factor CD121a CD62L (naïve: high, CD186 (CXCR6) CD294 (CRTH2)› CD161 BATF CD121b effector: low, CD191 (CCR1) CD365 (TIM1) CD194 (CCR4) Bcl-6 CD137 (4-1BB) memory: high) CD195 (CCR5) I L-25 R ( I L-17R B ) CD196 (CCR6)› c-Maf CD152 (CTLA-4) CD69h CD212 (IL-12Rβ1) IL-33R (ST2) C D 3 6 0 ( I L-21R ) IRF4 CD357 (GITR/AITR) CD127 (IL7Rα) CD218a (IL-18Rα) Intracellular/ CD212 (IL-12β1) STAT3 GARPh CD134 (OX40)h CD254 (RANKL) transcription factor I L-23 R LAPh CD137 (4-1BB)h CD366 (TIM3) BATF Intracellular/ TIGIT CD152 (CTLA-4)h Intracellular/ GATA-3› transcription factor Intracellular/ CD154 (CD40L)h transcription factor IRF4 AHR transcription factor CD272 (BTLA)h STAT1 STAT6 BATF c-Rel CD279 (PD-1)h STAT4 c-Maf Eos T-bet› IκBζ Foxp3› IRF4 Helios RORα STAT5 RORγt› STAT3

5 Human CD8 T cell markers

Pan markers Central memory Effector memory Effector Naïve CD2 Secreted Secreted Secreted Surface CD3 IFNγint Granzyme B CCL3 (MIP-1α) CD27 CD5 I L-2 int IFNγhigh CCL4 (MIP-1β) CD45RA CD7 TNFαint I L-2 low CCL5 (RANTES) CD62L CD8 Perforin Granzyme A CD127 (IL7Rα) Surface high CD25h TNFα Granzyme B CD197 (CCR7) low CD27 CCR7 Granzyme K CD27 Surface CD28 IFNγ CD28 CD44 CD45RO› I L-2 CD45RO CD62Llow Perforin CD62L› CD127 (IL7R )high TNFα CD62Llow α CD197 (CCR7)low CD127 (IL7Rα)high Surface KLRG1› low CD25h CD197 (CCR7) › KRG1high CD69h Intracellular/ Intracellular/ CD30 transcription factor transcription factor CD122h› Eomes Eomesint int CD137 (4-1BB) T-bet T-betint CD134 (OX40) CD223 (LAG-3) CD272 (BTLA) CD278 (ICOS) CD279 (PD-1) CD366 (TIM3) KLRG1› Intracellular/ transcription factor T-bet

6 Human NK/ILC markers

NK ILC1 ILC2 ILC3 LTi

Secreted Secreted Secreted Secreted Secreted Granulysin IFNγ CCL3 (MIP-1α) I L- 4 I L-17A I L-17 Granzyme A I L-10 IFNγ I L- 5 I L-17A F I L-2 2 Granzyme B Perforin I L- 9 I L-17F LTβ Surface Granzyme K TNFα I L-13 I L-2 2 TNFβ (LTα) Granzyme M CD56 Amphiregulin CD103 Surface Surface Surface Intracellular/ CD183 (CXCR3) Surface CD56 CD7 CD11b transcription CD335 (NKp46) CD25 CD117› CD25 CD16 factor CD336 (NKp44) CD45 C D127 ( I L-7R α)› CD117› CD56› E4BP4 CD337 (NKp30) CD117› CD335 (NKp46) C D127 ( I L-7R α) CD69h Eomes Linneg C D127 ( I L-7R α) CD336 (NKp44) CD161 CD94 GATA-3 CD161 Intracellular/ CD337 (NKp30) CD196 (CCR6) CD122 Id2 CD184 (CXCR4) transcription factor IL-23R CD252 (OX40L) CD161 Runx1 CD186 (CXCR6) neg Id2 Lin I L-23 R CD244› T-bet CD199 (CCR9) Tbet MHCII CD226 TOX CD278 (ICOS) Intracellular/ transcription factor CD314 (NKG2D) CD294 (CRTH2) Intracellular/ AHR transcription factor CD335 (NKp46) I L-25 R ( I L-17R B ) Id2 AHR CD336 (NKp44) IL-33R (ST2) CD337 (NKp30) Linneg RORγt Id2 CD158 family TSLP receptor T-bet RORγt TCF1 Runx1 KLRG1 Intracellular/ TIGIT transcription factor TOX Arginase-1 GATA-3 Gfi1 Id2 RORα TCF1

7 Human NKT/γδ T cell markers Pan markers γδ T cell NKT CD3 Secreted Secreted GM-CSF I L- 4h Granzyme B I L-10h IFNγ I L-13h I L- 4 IFNγh I L- 5 TNFαh I L-17A Surface Perforin CD4 TNFα CD8 Surface CD16 CD5 CD56 CD16 CD69 CD27 CD160 CD28 TCR Vα24› CD45RA Intracellular/ CD56 transcription factor CD57 PLZF CD62L Id2 CD69 CD70h CD107a CD314 (NKG2D) TCR γδ› TCR Vδ1 TCR Vδ2 TCR Vδ3

8 Human Macrophage/monocyte markers

Pan markers M2 macrophage M1 macrophage Monocyte Tissue associated CD11b Secreted Secreted Surface macrophage CD15 IDO IFNγ CD14› CD33 I L-10 I L-1α CD33 Surface CD40 TGFβ I L-1β CD172a (SIRPα) Axl CD63 I L- 6 CD192 (CCR2) CD68 (mature) Surface I L-12 CD14 CD80h CD115 I L-23 CD68 CD85 CD204 TNFα CD115 CD86h CD163 CD163 CD105h CD206 (MMR) Surface CD206 CD115 CD209 (DC-SIGN) CD16 CD369 (Dectin-1) CD169 FcεR1 CD16/CD32 HLA-DR CD195 (CCR5) VSIG4 CD32 CD273 (PD-L2) CD282 (TLR2) CD64 Intracellular/ Intracellular CD284 (TLR4) CD68 transcription factor NOS2 CD354 (Trem-1) IRF4 CD80 CXCL10 STAT6 CD86 CXCL11 CD369 (Dectin-1) CXCL9 Mer (MerTK) GPNMB MHCII Mature macrophage Intracellular/ marker transcription factor MIP-2α (CXCL2) IRF5 STAT1

9 Human Dendritic cell markers

Pan markers Conventional dendritic cell Plasmacytoid CD11b dendritic cell Secreted CD13 IDO Secreted CD33 I L-1β IFNα CD80h I L- 6 IFNβ CD83 I L- 8 CD86h I L-12 Surface MHCIIh I L-15 CD85g (ILT7) IL-23 CD123› Surface Intracellular/ CD283 (TLR3) CD1b transcription CD303 (BDCA-2)› CD1c (BDCA-1) factor CD304 (BDCA-4)› CD11c› CD135 CD370 (CLEC9A) CD289 (TLR9) CD14i Intracellular/ CD40h Fit3 transcription factor CD49d CD287 (TLR7) CD141 (BDCA-3) CD289 (TLR9) CD172a (SIRPa) E2-2 CD197 (CCR7) IRF8 CD205 (DEC-205) CD207 (Langerin) CD206 CD273 (B7-DC) CD282 (TLR2) CD284 (TLR4) CD369 (Dectin-1) CD370 (CLEC9A)

10 Human Granulocyte markers

Pan markers Mast Neutrophil Basophil Eosinophil CD11b Secreted Secreted Secreted Secreted CD13 Cathepsins Elastase I L- 4 MBPs CD15 Histamine Lactoferrin I L-13 EDN TNF CD32 α I L- 6 Histamine EPX CD33 I L- 4 I L-12 TGFß CCL3 (MIP-1α) TNFα Surface NGF I L-1 α/ß Surface CD9 Surface Basophil Marker CD284 (TLR4) CD15 Surface CD9 CD286 (TLR6) CD24 CD9 CD10 CD177 CD11a CD294 CD35 CD15 CD15 CD181 (CXCR1) CD13 FcεR1› CD24 CD17 CD282 (TLR2) CD43 CD16 CD35 CD24 CD284 (TLR4) Intracellular/ CD64 CD25 transcription CD43 CD35 CD286 (TLR6) CD116 CD33 factor CD64 CD43 Calprotectin CD123 CD38 C/EBPα CD116 CD66a (S100A8/A9) CD125› › CD43 CD281 (TLR1) CD117 (c-kit)› CD66b Intracellular/ CD126 CD66c CD88 CD289 (TLR9) CD123 transcription CD193 (CCR3)› CD66d factor CD123› GATA2 CD125 CD244 CD89 CD281 (TLR1) CD125 CD126 FcεR1 CD93 CD289 (TLR9) CD154 FcεR1› CD112 CD192 IL-33R (ST-2) CD114 CD203c CD116 CD218 (IL-18R) CD157 CD282 (TLR2)

11 Human Megakaryocyte/platelet markers

Platelet Megakaryocyte

Surface Surface CD9 CD41› CD23 CD42a CD31 CD42b› CD36 CD42c CD41 CD42d CD42a› CD49f CD42b› CD51 CD42c CD61 CD42d CD110 CD49f CD112 CD51 CD123 CD60a CD61 CD84 CD92 CD107ah CD107bh CD110 CD147 CD151 CD173 CD226 GARP LAP

12 Human Stem cell markers

Pan markers HSC CMP CLP CD34 Surface Surface Surface CD117 (c-kit) CD38low/neg CD33› CD7 CD164 CD59 CD45RA CD38 CD133› CD123 low CD45RA CD135 (Flt3) CD131 CD90low CD338 CD135 (Flt3) CD110 Linneg CD173 CD117 (c-kit) low Intracellular/ CD174 C D127 ( I L-7R α)› transcription factor Linneg HLA-DR neg GATA-3 Intracellular/ Lin TdT transcription factor Intracellular/ Ikaros transcription factor PU.1 Aiolos c-myb GATA-3 PU.1 TdT

13 Human

HumanOther cell type markers

Epithelial cells Erythrocytes Fibroblasts Neurons Stromal cells

CD24 CD45neg CD10 CD6 CD10 CD49f CD51 CD29 CD24 CD34 CD66a CD61 CD47 CD90 CD157 CD75 CD235a/glycophorin CD81 CD143 Podoplanin/p38 CD104 CD91 CD166 Stro-1 CD121a CD121a CD171 CD133 CD140a/PDGFRa CD200 CD167a CD140b/PDGFRb CD230 (prion protein) CD326 (EpCAM) Vimentin CD271 (NGFR) Cytokeratin CD304 (neuropilin) MHCII pos Nestin

Endothelial cells

Surface CD31 CD34 CD54 (ICAM-1) CD61 (Integrinβ3) CD62E (E-selectin) CD105 (Endoglin) CD106 (VCAM-1) CD144 (VE-cadherin) CD146 CD202b (Tie2) CD309 (FLK1) Podoplanin

14 Mouse

B cell markers

Pan markers Follicular Marginal zone Germinal center Plasma cell Memory CD20 Surface Surface Surface Surface Surface CD21/CD35low/neg CD20 CD1d CD19 CD9 CD38› CD22 CD21/CD35low/neg CD5low CD81 CD16/32 CD45R (B220)low CD23 CD22 CD9 CD95 CD19 low CD267 (TACI) CD24 (HSA)low CD23› CD21/CD35high› GL7› CD81 CD269 (BCMA) CD25h CD24 (HSA)low CD23low/neg IgG CD138› IgG CD36h CD25h CD24 (HSA)low IgG CD40h Intracellular/ CD36h CD36 Intracellular/ CD69h transcription factor Intracellular/ transcription factor CD40h CD81 CD80h AID transcription factor CBF1 CD69h CD180 (RP105) CD81 Bach2 BLIMP1 Spi-B CD80h CD363 (S1PR1) CD86h Bcl-6 IRF4 CD81 FcRL3 CD180 (RP105) IRF8 XBP-1 CD86h IgDlow/neg NF-кB CD252 (OX40 ligand)h CD180 (RP105) IgMhigh› high Pax5 IgD CD252 (OX40 ligand)h low/int Intracellular/ IgM IgDhigh› transcription factor IgMlow/int EBF1 Intracellular/ Notch2 transcription factor Bcl-6 EBF1 FoxO1 Ikaros Pax5

h = Upregulated i = Downregulated › = Key marker  = Subset

15 Mouse

CD4 T cell markers

Pan markers Th1 Th2 Th17 Tfh Treg CD3 Secreted Secreted Secreted Secreted Secreted CD4 IFNγ Amphiregulin GM-CSF I L-21 I L-10 CD5 I L-2 I L- 4 I L-17A TGFβ Surface CD7 TNFα I L- 5 I L-17A F CD185 (CXCR5)› Surface CD25h TNFβ (LTα) I L-10 I L-17F I L-13 I L-21 CD278 (ICOS)› CD25 CD27 Surface I L-2 2 CD279 (PD-1)› CD137 (4-1BB) CD28 CD94 Surface Intra/Txn CD39 CD44h CD119 (IFNγR1) CD184 (CXCR4) Surface BATF CD73 CD194 (CCR4) CD194 (CCR4) CD101 CD62L (naïve: high, CD183 (CXCR3)› Bcl-6 effector: low, CD186 (CXCR6) CD198 (CCR8) CD196 (CCR6)› c-Maf CD121a memory: high) CD191 (CCR1) CD294 (CRTH2) CD121a IRF4 CD121b CD365 (TIM1) CD212 (IL-12 1) CD152 (CTLA-4) CD69h CD195 (CCR5) β STAT3 CD212 (IL-12Rβ1) I L-25 R ( I L-17R B ) C D 3 6 0 ( I L-21R ) CD304 (Neuropilin-1)› CD127 (IL7Rα) CD218a (IL-18Rα) IL-33R (ST2)› I L-23 R CD357 (GITR) CD134 (OX40)h CD254 (RANKL) Intracellular/ Intracellular/ FR4 CD137 (4-1BB)h CD366 (TIM3) transcription factor transcription factor GARPh CD152 (CTLA-4)h Intracellular/ BATF AHR LAPh CD154 (CD40L)h transcription factor GATA-3› BATF TIGIT IRF4 c-Maf CD272 (BTLA)h STAT1 Intracellular/ STAT4 STAT6 IκBζ transcription factor CD278 (ICOS)h T-bet› IRF4 c-Rel CD279 (PD-1)h RORα Eos RORγt› Foxp3› STAT3 Helios STAT5

h = Upregulated i = Downregulated › = Key marker  = Subset

16 Mouse

CD8 T cell markers

Pan markers Central memory Effector memory Effector Naïve CD3 Secreted Secreted Secreted Surface CD5 I L- 4 low Granzyme B CCL3 (MIP-1α) CD62L CD8 IFNγlow IFNγ CCL4 (MIP-1β) C D127 ( I L-7R α) CD27 I L-2 CCL5 (RANTES) CD197 (CCR7) CD28 Granzyme A Surface Perforin CD183 (CXCR3) CD44 Granzyme B TNFα CD62L› IFNγ I L-2 C D127 ( I L-7R α) Surface Perforin CD197 (CCR7)high› CD44 TNFα Intracellular/ CD57 transcription factor KLRG1› Surface bcl-6 Intracellular/ CD25h Eomesint transcription factor CD30 int CD44 T-betint Eomes CD69h T-betint CD122h BLIMP1 CD134 (OX40) CD137 (4-1BB) CD223 (LAG-3) CD272 (BTLA-4) CD278 (ICOS) CD279 (PD-1) CD366 (TIM3) KLRG1 Intracellular/ transcription factor BLIMP1 Id2 T-bet

17 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

NK/ILC markers

NK ILC3 ILC2 ILC1 LTi

Secreted Secreted Secreted Secreted Secreted Granzyme A I L-10 I L-17A Amphiregulin IFNγ I L-17 Granzyme B Perforin I L-17A F I L- 4 CCL3 (MIP-1α) LTβ Granzyme K TNFα I L-17F I L- 5 TNFβ (LTα) I L- 9 Surface IFNγ I L-2 2 I L-13 CD103 Surface Surface Intracellular/ Surface CD183 (CXCR3) CD4 Surface CD11b transcription CD117 CD335 (NKp46)› CD25 factor CD25 CD16 C D127 ( I L-7R α) Linneg CD117 E4BP4 CD117 CD25 CD196 (CCR6)› C D127 ( I L-7R ) Ly-6A/E (Sca-1) C D127 ( I L-7R α)› Eomes α CD49b (DX5)› CD335 (NKp46) CD184 (CXCR4) CD185 (CXCR5) GATA-3 Intracellular/ CD94 I L-23 R CD186 (CXCR6) transcription factor CD186 (CXCR6) Id2 neg CD122 Lin CD199 (CCR9) Id2 CD196 (CCR6) Runx1 CD161c (NK1.1) Intracellular/ CD278 (ICOS) Tbet I L-23 R T-bet CD226 transcription factor CD294 (CRTH2)› MHCII TOX I L-25 R ( I L-17R B )› CD314 (NKG2D) AHR› Intracellular/ Linneg CD335 Id2 transcription factor Ly-6A/E (Sca-1) (NKp46)› ROR t AHR γ IL-33R (ST2) Ly-49 family T-bet TSLP receptor Id2 TIGIT TCF1 RORγt› Intracellular/ transcription factor Runx1 Arginase-1 TOX GATA-3 Gfi1 Id2 RORα› TCF1

18 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

NKT/γδ T cell markers

Pan markers γδ T cell NKT CD3 Secreted Secreted Granzyme B IFNγh IFNγ I L- 4h I L- 4 I L-10h I L- 5 I L-13h I L-17A I L-17h Perforin I L-21h TNFα I L-2 2h TNFαh Surface CD25h I L-1R1 Surface Intracellular/ CD27 NK1.1 (on C57BL/6) CD4 transcription CD28 TCR Vγ1 CD8 factor CD44 TCR Vγ4 CD44h PLZF CD45R (B220)h TCR Vγ5 CD49b Egr2 CD45RB TCR Vγ6 CD94 HEB CD62L TCR Vγ7 CD160 Hobit CD69h CD183 (CXCR3) Intracellular/ CD71h CD184 (CXCR4) transcription factor CD103 CD186 (CXCR6) Eomes CD122 CD314 (NKG2D) RORγt C D127 ( I L-7R ) DX5 α T-bet CD150 Ly-49 CD196 (CCR6) NK1.1 (on C57BL/6) TCR γδ › TCR Vα14›

19 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

Macrophage/monocyte markers

Pan markers M2 macrophage M1 macrophage Monocyte Tissue associated Axl Secreted Secreted Surface macrophage CD11b Arginase IFNγ Ly6c CD16/CD32 IDO I L-1 CSF1R Surface CD40 I L-10 I L- 6 Axl CD64 TGFβ I L-12 CD192 (CCR2) CD68 YM1 I L-23 CD14 CD107 (Mac3) TNFα CD68 CD115 Surface CD115 CD282 (TLR2) CD14 Surface CD163 CD284 (TLR4) CD115 CD14 CD206 F4/80 CD163 CD16/CD32 CD369 (Dectin-1) Galectin-3 (Mac2) CD204 CD32 HLA-DR GITRL CD206 (MMR) CD64 CD273 (PD-L2) GPNMB CD209 (DC-SIGN) CD68 Intracellular Ly6G (Gr-1) CSF1R CD80 NOS2 MHC Class IIh FcεR1 CD86 Ly-6C CD204 Intracellular/ CD369 (Dectin-1) transcription factor Ly-6C IRF4 Mer (MerTK) RELMα MHCII STAT6 Intracellular/ transcription factor IRF5 NOS2 STAT1

20 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

Dendritic cell markers

Pan markers Conventional dendritic cell Plasmacytoid CD11c dendritic cell Secreted CD80h IDO Secreted CD86h I L-1β IFNα MHCIIh I L- 6 IFNβ I L- 8 I L-12 Surface I L-15 CD45R (B220) I L-23 CD317 (BST2, PDCA-1)› Surface Intracellular/ Siglec-H› CD4 transcription Intracellular/ CD8  factor transcription factor low CD135 (Flt3) CD11b  CD287 (TLR7) CD183 (TLR3) CD40h CD289 (TLR9) CD289 (TLR9) CD83 E2-2 CD197 (CCR7) IRF8 CD205 (DEC-205)› CD207 (Langerin) CD209 (DC-SIGN) CD273 (B7-DC) CD369 (Dectin-1) CD370 (CLEC9A) DCIR2

21 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

Granulocyte markers

Pan markers Mast Neutrophil Basophil Eosinophil CD11b Secreted Secreted Secreted Secreted CD16/CD32 Cathepsins Elastase I L- 4 EPX Histamine Lactoferrin I L-13 MBPs I L- 6 TNFα Histamine I L-12 Surface I L- 4 CCL3 (MIP-1α) TNFα CD9 TGFß I L-1 α/ß Surface CD15 NGF CD9 Surface CD24 Surface CD11a CD35 CD10 CD13 CD9 CD15 CD43 CD25 CD15 CD17 CD64 CD33 CD24 CD24 CD38 CD116 CD35 CD35 CD43 CD123 CD43 CD43 CD125 CD66a› CD88 (C5a receptor) CD64 CD126 CD66b CD123 CD116 CD66d CD125 CD170 (SiglecF)› CD117 (c-kit)› CD89 CD154 (CD40 ligand) CD193 (CCR3)› CD123 CD93 CD192 (CCR2) CD244 CD125 CD112 (Nectin-2) CD218 (IL-18 receptor) FcεR1 CD126 CD114 (G-CSFR) CD282 (TLR2) FcεR1› CD116 CD284 (TLR4) CD286 (TLR6) IL-33R (ST2) CD157 CD177 CD294 (CRTH2) CD181 (CXCR1) FcεR1› CD282 (TLR2) Intracellular/ CD284 (TLR4) transcription factor CD286 (TLR6) CD281 (TLR1) Ly-6G (Gr-1)› CD289 (TLR9) Intracellular/ C/EBPα transcription factor GATA-2 CD281 (TLR1) CD289 (TLR9)

22 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

Megakaryocyte/platelet markers

Platelet Megakaryocyte

Surface Surface CD9 CD9 CD23 CD31 CD41 CD41› CD42a, b, and c CD42b CD43 CD51 CD44 CD61 CD47 CD62Ph CD49b CD93 CD49f CD147 CD51 CD151 CD61 CD154 CD63 GARP CD69 LAP CD93 CD62Ph CD107ah CD107bh CD110 CD147 CD151 GARP LAP

23 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

Stem cell markers

HSC CMP CLP

Surface Surface Surface CD34 CD34 CD34 CD38low/neg CD123 (IL-3R)› CD43 CD59 Linneg CD135 (Flt3) CD117 (c-kit)› Intracellular/ C D127 ( I L-7R α)› CD133 (prominin-1) transcription factor Linneg CD135 (Flt3) Ikaros Intracellular/ CD150 (SLAM)› PU.1 transcription factor Linneg Aiolos Ly-6A/E (Sca-1)› c-myb Intracellular/ GATA-3 transcription factor PU.1 GATA-2 TdT TdT

24 h = Upregulated i = Downregulated › = Key marker  = Subset Mouse

Other cell type markers

Epithelial cells Erythrocytes Fibroblasts Neurons Stromal cells

CD24 CD24 CD10 CD6 CD10 CD49f CD45neg CD29 CD24 CD34 CD66a CD51 CD47 CD90 CD157 CD75 CD61 CD81 CD143 Podoplanin/p38 CD104 Ter119 CD91 CD166 Stro-1 CD121a CD121a CD171 CD133 CD140a/PDGFRa CD200 CD167a CD140b/PDGFRb CD230 (prion protein) CD326 (EpCAM) Vimentin CD271 (NGFR) Cytokeratin CD304 (neuropilin) MHCII pos Nestin

Endothelial cells

Surface CD31 (PECAM-1) CD34 CD54 (ICAM-1) CD61 (Integrinβ3) CD62E (E-selectin) CD105 (Endoglin) CD106 (VCAM-1) CD144 (VE-cadherin) CD146 CD202b (Tie2) CD309 (FLK1) Podoplanin VEGF receptor 3

25 h = Upregulated i = Downregulated › = Key marker  = Subset