Wheat Inf. Serv. 123, 2017. www.shigen.nig.ac.jp/ewis

Wheat Information Service Electronic Newsletter for Wheat Researchers

No. 123

CONTENTS

Meeting Report

Kazuhiro Sato The Eleventh Triticeae Meeting of Japan, 2016.

Others

Instructions to Authors.

Wheat Inf. Serv. 123: eWIS 123.1, 2017. www.shigen.nig.ac.jp/ewis

Meeting Report

The Eleventh Triticeae Meeting of Japan, 2016

Kazuhiro Sato Institute of Plant Science and Resources, Okayama University, Kurashiki, 710-0046, Japan Corresponding author: Kazuhiro Sato (E-mail: [email protected])

The Eleventh Triticeae Meeting of Japan was held O04.* Morphological variations of starch at Institute of Plant Science and Resources, grains in cereals Okayama University, Kurashiki on December 10 and 11, 2016 as a joint program of the Chugoku Ryo Matsushima branch meeting of Japanese Society of Breeding. IPSR, Okayama U., Japan A total of 156 attendee including researchers and students participated in the meeting (Fig. 1). This O05. Barley genome information meeting includes a session of National BioResource Project (NBRP) - Barley and Wheat. Kazuhiro Sato The objectives of the meeting were to share the IPSR, Okayama University, Japan information about genetic and genomic resources in Triticeae and to discuss their efficient use in the A BAC physical map and sequences in gene space research community. The meeting had 13 oral and have been released in 2012 by the international 64 poster presentations as shown below. The next consortium. The continuing efforts of BAC based meeting will be held at Kyoto University next analysis for individual chromosome have been year. done by contributing organizations and will be published after the end of 2016. The sequences ABSTRACTS & TITLES are already available from the database at IPK, Germany under registration and acceptance of Oral Presentation condition for uses. IPK has started sequencing 20,000 genebank accessions by GBS method and O01.* The current challenges and future might finish most of them. The large exome prospects of plant genome editing. – How to capture based sequencing is also underway at JKI, apply genome editing into crop breeding? – UK. The present status of barely genome analysis and its future is discussed in the talk. Keishi Osakabe Fac. Biosci. Bioind,, Tokushima Univ., Japan O06. Impact of wheat lines with related wild species on heat stress tolerance O02.* Plant artificial chromosomes: Present and future Keiichi Mochdia1, 2, 3 1CSRS, RIKEN, Japan Minoru Murata 2KIBR, Yokohama City U., Japan IPSR, Okayama U., Japan 3IPSR, Okayama U., Japan

O03.* Genetic study and improvement of In the family Poaceae, Brachypodium is a capsaicinoid and its analogs with chili pepper temperate grasses of the Pooideae subfamily that bio-resource. includes some major crops such as wheat, barley and rye. The close phylogenetic relation between Yoshiyuki Tanaka Brachypodium and the Triticeae species suggests Okayama U. Japan that the Brachypodium genome and associated

resources will be beneficial for gene discovery and structural genomic studies in grasses. Masatomo Kobayashi Recently developed Brachypodium resources are RIKEN BioResource Center, Japan compared to those of wheat and barley, to promote the mutual access to Poaceae resources. During recent years, life science is making remarkable progress by the emergence of novel O07. Current status of the reference genome technologies such as next generation sequencer sequencing of wheat and genome editing technology. However, utilization of genome editing technology in plant Hirokazu Handa science is less significant than that in animal NARO Institute of Crop Science, Japan science. The reasons are as follows: 1) Embryo manipulation technology that is The International Wheat Genome Deciphering indispensable for efficient application of Consortium (IWGSC) was established in 2005 to genome editing technology has not been create the reference genome sequence of wheat. established yet in plants. After more than 10 years’ efforts of IWGSC, we 2) Thus genome editing is carried out using are in the final step towards the creation of the transformation technology in plants. wheat reference genome sequence. In this 3) However, transformation efficiency is presentation, the author presented the current relatively low in most of crops. status of wheat genome sequencing by IWGSC, 4) Also, transformation technology is under the especially the recent change of IWGSC's strategy strict control by the Cartagena Law. by the introduction of new assembly technique In order to overcome these disadvantages, it is (DeNovo MAGIC ™, NRgene). And also the important to utilize experimental plants that are author introduced information about the research easy to handle in the laboratory. We introduce movement after completion of the reference Brachypodium distachyon as an experimental genome sequence. plant of monocot that has short life cycle and is able to grow in the laboratory conditions. We are O08. Prospects of crop research in the genome developing resources, technologies and editing technology era - utilization and information of Brachypodium in order to fully development of resources and technologies of utilize this plant in the crop science, especially model plants and Pooideae wheat and barley.

Fig. 1 The Eleventh Triticeae Meeting of Japan, 2016

O09. Barley mutants: powerful aids for gene of water is significantly increased compared with identification control cultivar. Our study indicates that the enhancement of ABA receptor expression Shin Taketa contributes to not only drought tolerance but also Institute of Plant Science and Resources, the “water-saving drought tolerance” phenotype Okayama University, Japan which can perform highly efficient seed production with limited water. The history and current status of barley mutant researches in Kurashiki are briefly summarized. O11. Construction of genome sequence of International research trends of barley mutants are wheat chromosome 6B and its application for briefly summarized, with particular emphases on wheat study their effective uses in validation of gene cloning and biological analysis of gene functions. My Fuminori Kobayashi, Hirokazu Handa personal perspective is that molecular dissections Institute of Crop Science, NARO, Japan of barley under the international standard of rice or maize, should drastically improve the quality For better understanding the genome structure of of barley science. wheat and accelerating the gene isolation and breeding for wheat, our research group, as a O10. Enhancement of ABA receptor function member of International Wheat Genome confers water-saving drought tolerance in Sequencing Consortium (IWGSC), constructed wheat the pseudomolecule of wheat chromosome 6B of Ryosuke Mega1 Fumitaka Abe2 June-Sik Kim3, ‘Chinese Spring’ (CS) by the BAC-by-BAC Keisuke Tanaka4, Hisato Kobayashi4, Yoichi sequencing approach. Genome assemblies Sakata5, Hisashi Tsujimoto1, Kousuke Hanada6 constituting the pseudomolecule were highly and Masanori Okamoto1,7 ordered along the chromosome 6B by conducting 1ALRC, Tottori University, Japan the scaffolding and radiation hybrid mapping. 2 Institute of Vegetable and Floriculture Science, This genome sequence for chromosome 6B NARO, Japan allowed us to study a genomic region for the leaf 3CSRS, RIKEN, Japan rust resistance gene, LrRW12. The LrRW12 was 4NODAI Genome Research Center, Japan genetically mapped on the long arm of 5Applied Bioscience, Tokyo University of chromosome 6B by the GBS approach. The SNP Agriculture, Japan markers were developed along the 6Frontier Research Academy for Young pseudomolecule, and finally about 4 Mb region Researchers, Kyushu Institute of Technology, was identified to include LrRW12. Using the Japan genome sequence for this region, we could predict 7PRESTO, JST, Japan gene loci and detect several SNPs in these loci between resistant and susceptible accessions by Global climate change has accelerated water transcriptome data. In this way, application of scarcity followed by huge drought, which led to genome sequence highly facilitates the estimation massive loss of crop production and may threaten of target genomic region and its structural food security in many countries. Therefore, a analysis, even in common wheat. This work was drought tolerant trait which can save water supported by grants from the Ministry of consumption (water-saving) is being demanded Agriculture, Forestry and Fisheries of Japan recently. Abscisic acid (ABA) synthesis is (NGB1003) and JSPS KAKENHI Grant Number induced by drought stress. Consequently, stomatal JP24780008. closure is facilitated to suppress transpiration via ABA signaling pathway. This pathway is O12. Genome editing in plants and barley commonly found in terrestrial plants, modulating transformation PYR/PYL can be a good option to improve crop drought tolerance. However, since wheat genome Hiroshi Hisano database has not been established yet, wheat ABA Institute of Plant Science and Resources, receptors (TaPYLs) were not completely Okayama University, Japan identified. To improve wheat drought tolerance, we characterized TaPYLs from wheat genome and Genome editing is a new technology to produce generated the transgenic wheat overexpressing mutants by designer nucleases e.g. Transcription wheat PYR/PYL (TaPYLox). In TaPYLox, Activator-Like Effector Nucleases (TALEN) and biomass amount and seed yield produced from 1L Clustered Regularly Interspaced Short

Palindromic Repeats/ CRISPR-associated 9 introduction of the genes related to pasta quality (CRISPR/Cas9). In this presentation, the brief from durum wheat. overview of genome editing was explained, and it included about particular formation of TALEN Poster Presentation and CRISPR/Cas9, differences of procedure for genome editing between in animals and in plants, P01.* comparison between conventional transformation QTL analysis of α-amylase activity in barley and genome editing, and mechanisms of knock-in Matsumoto, S.1, H. Hisano1, M. Kihara2, T. S. and knock-out in target genes. Also published Zhou2 and K. Sato1 (1IPSR, Okayama U., Japan reports and current states of genome editing in 2BRDD, Sapporo Brew. Ltd., Japan) plants was inducted. On the other hand, author presented about a P02.* project for genome editing in barley. Then author Barley albino lemma mutant: molecular genetic introduced about identification of the genomic analysis and spike-photosynthesis measurement region responding to transformation amenability Hattori, M., T. Takami, W. Sakamoto and S. in barley. The efficiency of transformation is Taketa (IPSR, Okayama U.) important for current genome editing in plants to introduce the genes encoding those nucleases. P03.* Author found several loci showing segregation Genetic linkage analysis for identifying root-knot distortion of SNPs markers in transgenic barley nematode (Meloidogyne incognita) resistance plants derived from a cross between cvs. Haruna gene in sweetpotato Nijo as recalcitrant of transformation and Golden Kishimoto, K1, K. Shirasawa2, R. Sasai1, A. Promise as reliable for transformation. These loci Kuramoto3, S. Isobe2, M. Tahara1, Y. Okada4, H. could be necessary or advantageous for Tabuchi4, A. Kobayashi4 and Y. Monden1 (1Grad. transformation in barley. Sch. Environ. Life Sci., Okayama U., 2Kazusa DNA Res. Inst., 3Grad. Sch. Agr., Kyoto U., O13. Durum wheat breeding in WARC/NARO 4KONARC)

Mikiko Yanaka P04.* Western Region Agricultural Research Center, 16S metagenomics analysis of microbiome NARO, Japan communities in root-knot nematode (Meloidogyne incognita) and Streptomyces ipomoea infected Durum wheat (Triticum turgidum L. ssp. durum) soils is mainly used for pasta production. It has not Nakashima, H.1, T. Ishige2, T. Kuranouchi3, Y. been cultivated in Japan because of its late Momota4, K. Yonemoto5, M. Tahara1 and Y. maturity and its very weak resistances to Monden1 (1Grad. Sch. Environ. Life Sci., Fusarium head blight (FHB) and pre-harvest Okayama U., 2NODAI Genome Research Center, sprouting (PHS), compared with bread wheat (T. 3NICS, 4CARC, 5TAFFTSC) aestivum L.). However, a demand for pasta made from domestic durum wheat is increasing in Japan. P05.* WARC/NARO thought that Setouchi area seems Identifying genomic regions associated with to be most preferable for durum wheat cultivation Streptomyces ipomoeae pathogen resistance by in Japan because it has less rainfall during the using SNP data cultivation, and then started durum wheat Aikawa, Y.1, K. Shirasawa2, A. Kuramoto3, Y. breeding in the late 1990s. In 2015, Imai4, S. Isobe2, M. Tahara1, Y. Okada5, O. WARC/NARO released a new durum wheat Jahana6 and Y. Monden1 (1Grad. Sch. Environ. cultivar named ‘Setodure’, which is a first durum Life Sci., Okayama U., 2Kazusa DNA Res. Inst., wheat cultivar in Japan, after the evaluation of its 3Grad. Sch. Agr., Kyoto U., 4Tottori U. Technical pasta quality by Nippon Flour Mills. ‘Setodure’ dept., 5KONARC, 6OPARC) showed maturity similar to that of the bread wheat cultivar ‘Norin61’, weak resistances to FHB and P06.* PHS and pasta quality which is superior to bread Experimental assessment of simplified cultivar wheat, but inferior to the imported durum wheat identification method in azuki processed products brand. WARC/NARO is trying to improve the using single tag hybridization (STH) resistances to PHS or FHB by the introduction of chromatographic printed array strip (PAS). the reported resistance genes or QTLs from bread Sasai, R.1, M. Tahara1, K. Takasaki2 and Y. wheat and to improve the pasta quality by the Monden1 (1Grad. Sch. Environ Life. Sci.,

Okayama U., 2FASMAC Co., Ltd.) P13.* Molecular genetic analysis of the rice stay-green P07.* mutant dcd1 Development of cultivar identification DNA Yamatani, H.1, K. Kohzuma1,2, M. Nakano1, Y. markers for purple sweetpotato using Hayashi3, T. Takami4, Y. Kato4, Y. Monden5, T. retrotransposon insertion polymorphisms Kumamaru6, Y. Okumoto7, W. Sakamoto2,4, T. Ono, N.1, K. Kishimoto2, M. Tahara2 and Y. Abe3 and M. Kusaba1,2 Monden2 (1Fac. Agr., Okayama U., 2Grad. Sch. (1Grad. Sch. Sci., Hiroshima U., 2CREST, Env. & Life Sci., Okayama U.) 3RIKEN, Nishina Cent, 4Inst. Plant Sci. Res., Okayama U., 5Grad. Sch. Environ. Life Sci., P08.* Okayama U., 6Fac. Agr., Kyusyu U., 7Grad. Agr., Morphological characteristics in F1 populations Kyusyu U.) derived from cultivated and wild sweetpotato strains P14.* Kimura, T.1, M. Tanaka2, S. Isobe3, M. Tahara4 Map-based cloning in the genus Chrysanthemum and Y. Monden4 (1Fac. Agr., Okayama U., Aruga, Y.1, M. Nakano1, T. Kozuka1, S. Isobe2, K. 2KONARC, 3Kazusa DNA Res. Inst., 4Grad. Sch. Taniguchi1 and M. Kusaba1 (1Lab. Plant Environ. Life Sci., Okayama U.) Chromosome Gene Stock, Hiroshima U., 2 Kazusa DNA Res. Inst.) P09.* Introduction of chromosome arm 1EL partial P15.* region with high-molecular-weight glutenin Molecular genetic analysis of leaf senescence subunit genes associated with strong dough into regulated by blue light common wheat by homoeologous chromosome Shimono, Y.1, T. Kozuka1, R. Inoue1, M. Kusaba1,2 recombination (1Hiroshima U., 2CREST) Hayashi, N. and H. Tanaka (Fac. Agr., Tottori U.) P16.* P10.* Mechanisms of functionalization in plant MSD population: New source of heat tolerance of duplicate genes wheat in high-temperature environments Ezoe, A.1, K. Shirai1, K. Hanada1 (1Kyushu Inst. Elbashir, A. A. E.1,3, Y. S. A. Gorafi2,3, I. S. A. Tech.) Tahir3, A. M. A. Elhashimi3, M. G. A. Abdalla3 and H. Tsujimoto2 P17.* (¹United Grad. Sch. Agr. Sci., Tottori U., Japan, Search novel hormone like peptides in ²Arid Land Res. Center, Tottori U., Japan, ³Agr. Arabidopsis thaliana by treatment assay Res. Corp., Wad Medani, Sudan) Torii, R.1, Y. Kim1, T. Takeda1, M. Higuchi2, I. Obayashi1, M. Okamoto3, M. Shimizu2, T. P11.* Yoshizumi2, K. Nakaminami2, R. Nishi2, K. Compensational application of Leymus racemosus Shinozaki2, M. Seki2, M. Matsui2 and K. markers to analyze genetic diversity in tribe Hanada1,2 (1Kyushu Inst. Tech., 2RIKEN, 3Tottori Triticeae U.) Edet, O.1,2,5, J.-S. Kim3, K. Hanada4, M. Okamoto1 and H. Tsujimoto1 P18.* (1Arid Land Res. Center, Tottori U., Japan, Exhaustive search of alternative splicing changes 2United Grad. Sch. Agr. Sci., Tottori U., Japan, under shade avoidance in Arabidopsis thaliana 3RIKEN Center for Sustainable Res. Sci., Japan, throughout next generation sequencing analysis 4Dept. Biosci. Bioinformatics, Kyushu Inst. Tech., Nose, T.1, K. Shirai1, F. Christian2, T. Matsushita3 Japan, 5Dept. Crop Sci, U. Calabar, Nigeria) and K. Hanada1 (1Kyushu Inst. Tech., 2Lausanne U., 3Kyushu U.) P12.* Molecular phylogenetic analysis of some crops P19.* using 26S rRNA and ribulose1, 5-bisphosphate Transcriptome analysis of Leymus racemosus carboxylase/oxygenase (Rubisco large subunit with Next-generation sequence genes) Takeda, T.1, K. Tanaka2, H. Kobayashi2, H. Hassan, E. M., G. H. Badawi and M. H. Eltahir Tsujimoto3, M. Okamoto3 and K. Hanada1 (Fac. Agr., U. Khartoum, Sudan) (1Kyushu Inst. Tech., 2Tokyo U. Agr, 3Tottori U.)

P20.* Search for novel hormone-like peptides involved P27.* in the regulations of initial growth and flowering Genetic diversity analysis of melon germplasm time in Arabidopsis thaliana collection using GBS Yano, T.1, R. Torii1, I. Obayashi1, K. Shirai1, A. Shigita, G.1, M. N. Pervin1, H. Nishida1, Y. Ezoe1 and K. Hanada1 (1Kyushu Inst. Tech.) Monden1, M. Sugiyama2, K. Tanaka3 and K. Kato1 (1Grad. Sch. Environ. Life Sci., Okayama P21.* U., 2NIVFS, 3Fac. Agr. Life Sci., Hirosaki U.) Genetic analysis on heading time in RILs population derived from a cross of barley P28.* cultivars “Kashimamugi” and “Ishukushirazu” Fine mapping of the ‘Chogokuwase (extra-early Nishida, H.1, S. Yokota2, E. Aoki3 and K. Kato1 flowering)’ gene in wheat (1Grad. Sch. Environ. Life Sci., Okayama U., 2Fac. Sato, H.1, A. Ebara1, G. K. M. N. Haque2, H. Agr. Okayama U., 3NICS) Masuda2, H. Yamashita2, H. Nishida2, N. Mizuno3, M. Fujita4, S. Nasuda3 and K. Kato2 (1Fac. Agr., P22.* Okayama U. 2Grad. Sch. Environ. Life Sci., Field survey of Cucumis species in western part Okayama U., 3Grad. Sch. Agr., Kyoto U., 4NICS) of Nepal Thuy, D. T.1, K. Yashiro2, K. Shimomura3 and K. P29.* Kato1) (1Grad. Sch. Environ. Life Sci., Okayama Mapping of a novel flowering time QTL on 2HS U., 2Ibaraki Agr. Center, Plant-Biotech. Inst., chromosome derived from a barley cultivar 3NIVFS) Morex Yokota, S.1, M. Irisawa1, R. Tanabe2, H. Nishida2, P23.* E. Aoki3 and K. Kato2 (1Fac. Agr., Okayama U., Effect of interaction between LUX/PCL1 2Grad. Sch. Environ. Life Sci., Okayama U., 3 genotypes on heading time of wheat, revealed by NICS) the analysis of a wheat DH population derived from "Chogokuwase" and "Kinuiroha" P30. Haque G. K. M. N.1, H. Nishida1, H. Matsunaka2, Breeding of barley with new useful quality in M. Seki3, N. Mizuno4, M. Fujita5, S. Nasuda4 and KONARC. K. Kato1 (1Grad. Sch. Environ. Life Sci., Tonooka, T., M. Taira and T. Sugita (Kyushu Okayama U., 2KARC/NARO, 3CARC/NARO, Okinawa Agric. Res. Cent., NARO) 4Grad. Sch. Agr., Kyoto U., 5NICS) P31. P24.* Reproductive isolation between two wild einkorn Interspecific hybrids obtained by crossing wheat species cultivated melon and wild species of Cucumis Takumi, S. (Grad. Sch. Agr. Sci., Kobe U.) Pervin, M. N.1, G. Shigita1, T. Ishikawa2, K. Sakamoto1, T. P. Dung1, K. Tanaka3, H. Nishida1 P32. and K. Kato1 (1Grad. Sch. Environ. Life Sci., Evaluation of efficiency of transient expression in Okayama U., 2Ibaraki Agr. Center, Plant-Biotech. the wheat leaf using Gene Gun System GDS-80 Inst., 3Fac. Agr. Life Sci., Hirosaki U.) Mizoo, N. and K. Yoshida (Org. Adv. Sci. Tech., Kobe U.) P25.* Expression analysis on flowering-related genes by P33. RNAseq in a wheat breeding line "Chogokuwase" Application of RNA-seq-based BSA to fine and its progenitor lines mapping in synthetic hexaploid wheat Masuda, H.1, H. Nishida1, N. Mizuno2, S. Nasuda2, Nishijima, R.1, K. Sakaguchi1, K. Yoshida2, K. M. Fujita3 and K. Kato1 (1Grad. Sch. Environ. Sato3 and S. Takumi1 (1Grad. Sch. Agr. Sci., Kobe Life Sci., Okayama U., 2Grad. Sch. Agr., Kyoto U., 2Org. Adv. Sci. Tech., Kobe U., 3IPSR, U., 3NICS) Okayama U.)

P26.* P34. A study directed to uncover the origin of a Application of the genome-wide polymorphisms vernalization response gene Vrn-D4 in wheat to gene mapping in Aegilops umbellulata Harada, H., H. Nishida and K. Kato (Grad. Sch. Okada, M.1, K. Yoshida2, K. Sato3 and S. Takumi1 Environ. Life Sci., Okayama U.) (1Grad. Sch. Agr. Sci., Kobe U., 2Org. Adv. Sci.

Tech., Kobe U., 3IPSR, Okayama U.) Selection of high-efficiency gRNA for genome editing of Triticum aestivum L. using P35. CRISPR/Cas9 system RNA-seq analysis of near-isogenic lines for Inomata, M., Y. Kamiya, K. Kawaura and M. chlorina mutations in tetraploid wheat Isshiki (KIBR, Yokohama City U.) Nishigaki, K.1, K. Yoshida2, K. Sato3, N. Watanabe4 and S. Takumi1 (1Grad. Sch. Agr. Sci., P43 Kobe U., 2Org. Adv. Sci. Tech., Kobe U., 3IPSR, Study on traits showing heterosis in synthetic Okayama U., 4Fac. Agr., Ibaraki U.) hexaploid wheat Watanabe, R., Y. Jung, Y. Ogihara and K. P36. Kawaura (KIBR, Yokohama City U.) Screening of NAC and MYB transcription factor genes related to cell death in common wheat P44 Hosokawa, S.1, K. Yoshida2 and S. Takumi1 Identification of genes for sham ramification in (1Grad. Sch. Agr. Sci., Kobe U., 2Org. Adv. Sci. wheat Tech., Kobe U.) Mitsuhashi, Y.1, S. Sakuma2,3 and K. Kawaura1 (1KIBR, Yokohama City U., 2IPK, 3Tottori U.) P37. Genetic analysis of domestication related traits in P45 emmer wheat: seed morphology and weight QTL analysis of salt-tolerance in Miyazaki, Y.1, Pham Minh Ngoc1, Liberatore Shirasagi-komugi using Rad-Seq method Katie2, Shahryar Kianian2, N. Mori1 (1Lab. Crop Matsuda, S.1, A. Tokunaga1, S. Sakuma1,2,3, Y. Evol., Grad. Sch. of Agric. Sci., Kobe U., Ogihara1, A. J. Nagano4 and K. Kawaura1 (1KIBR, 2USDA-ARS Cereal Disease Lab., U. Minnesota) Yokohama City U., 2IPK, 3 Tottori U., 4Ryukoku U.) P38. Quantitative analysis of intra-molecular P46 recombination of mitochondrial genome in wheat Evaluation of salt tolerance in synthetic hexaploid Ohta, S.1, M. Makita1, M. Tsujimura2, T. Terachi2 wheat produced by CIMMYT and N. Mori1 (1Lab. Crop Evol., Grad. Sch. Agric. Tadokoro, M.1, A. Tokunaga1, S. Sakuma1,2,3 and Sci., Kobe U., 2Fac. Life Sci., Kyoto Sangyo U.) K. Kawaura1 (1KIBR, Yokohama City U., 2IPK, 3Tottori U., 4CIMMYT) P39. Genetic analysis of the domestication related P47 traits using backcross derived lines in emmer Genetic analysis of putative suppressor of wheat. α-gliadins in bread wheat Shimada, S.1, K. Gyu1, C. Vladutu1, T. Ishii2, S. Suzuki, S., Y. Kamei, M. Miura and K. Kawaura Kianian3, N. Mori1 (1Lab. of Crop Evol., Grad. (KIBR, Yokohama City U.) Sch. Agr. Sci., Kobe U., 2Lab. of Plant Breed., Grad. Sch. Agr. Sci., Kobe U., 3USDA-ARS P48 Cereal Disease Lab., U. Minnesota, U. S. A) KODA mediated changes in Japan wheat core collection harvest in the field with low fertilizers P40 Sekine, A.1, E. Haque1, S. Taniguchi1, S. Ogawa2, Toward the QTL analysis of grain dimensions and K. Takagi2, M. Yokoyama1 and T. Ban1 dormancy in wild emmer wheat (Triticum (1KIBR.Yokohama City U., 2Tech. Eng. Center, turgidum ssp. dicoccoides) Shiseido Co., Ltd) Terada, N.1, N. Mori1 and S. Ohta2 (1Lab. Crop Evol., Grad. Sch. Agr. Sci., Kobe U., 2Dept. P49 Biosci., Fukui Pref. U.) Identification of florigen gene family in breed wheat and their expression profiling in the P41 synthetic wheat Molecule breeding of salt-tolerant wheat using Mitsuhashi, A.1, R. Shimizu2, S. Takumi3, K. RNAi Shimizu2, H. Tsujimoto4, T. Ban1 and H. Tsuji1 Ishihara, M., K. Kawaura and M. Isshiki (KIBR, (1KIBR, Yokohama City U., 2U. Zurich, 3Grad. Yokohama City U.) Sch. Agr. Sci., Kobe U., 4Arid Land Res. Cent., Tottori U.) P42

P50 Identification of FT genes to reveal the P58 mechanism for bulb formation of Hordeum AVR effectors of barley powdery mildew are bulbosum secreted from the appressorium Arai, Y., K. Taoka and T. Ban (KIBR, Yokohama Inoue, C., M. Wahara, T. Kohguchi, A. Nakamura, City U.) H. Takei, K. Kobayashi, N. Yamaoka and T. Yaeno (Fac. Agr., Ehime U.) P51 Elucidation of epigenetic regulation mechanism P59 of flowering promote gene VRN1 Verification of the cytoplasmic effects on Umekita, K.1, K. Nagaki2, M. Murata2 and K. agricultural traits of common wheat: submergence Murai1 (1Dep. Biosci., Fukui Pref. U., 2IPSR, stress tolerance Okayama U.) Nakamura, C. and S. Takenaka (1Fac. Agr., Ryukoku U.) P52 Epigenetic regulation of floral MADS-box genes P60 in polyploid wheat Development of a RH mapping population for the Kuwabara, T.1, K. Nagaki2, M. Murata2 and K. Gc2-4Ssh gene in wheat. Murai1 (1Dep. Biosci., Fukui Pref. U., 2IPSR, Sakai, N., M. Yoshioka, N. Mizuno and S. Okayama U.) Nasuda (Lab. Plant Genet., Kyoto U.)

P53 P61 Analysis of amino acid sequence variation among Genetic mapping of a gametocidal gene Gc2-4Ssh VRN1 proteins and construction of VRN1 protein in wheat expression system using E.coli Yoshioka, M.1, N. Mizuno1, N. Sakai1, B. Friebe2 Tanaka, C. and K. Murai (Dep. Biosci., Fukui and S. Nasuda1 (1Lab. Plant Genet., Kyoto U., Pref. U.) 2WGRC, KSU)

P54 P62 Influences of Aegilops mutica cytoplasm on the A preliminary study on the zygotic induction of agronomic characters of Japanese wheat cultivars chromosome breakage by gametocidal genes in Matsumura, M. and K. Murai (Dep. Biosci., Fukui wheat Pref. U.) Yamada, H. and S. Nasuda (Lab. Plant Genet., Kyoto U.) P55 Genomic cross-talk mechanism among P63 homoeologous genes for photoperiod pathway in Copy number estimation of the rRNA repeats at bread wheat the Nor-2 locus on wheat chromosome 6B Mizuuchi, Y., T. Oyama and K. Murai (Dep. Murata, K.1, F. Kobayashi2, H. Handa2 and S. Biosci., Fukui Pref. U.) Nasuda1 (1Lab. Plant Genet., Kyoto U., 2NIAS, NARO) P56 Variation of heading dates and morphology in P64 Brachypodium distachyon collected from the The research of genome functions involved in eastern Mediterranean region environmental adaptation in allopolyploid plants Hibino, K. and S. Ohta (Dep. Biosci., Fukui Pref. Takahagi, K.1,2,3, K. Inoue3, R. Nakayama2,3, M. U.) Shimizu2,3, Y. Uehara-Yamaguchi3, Y. Onda2,3, K. Shinozaki3 and K. Mochida1,2,3,4 (1Grad. Sch. P57 Nano, Yokohama City U., 2KIBR, Yokohama City Studies on effector proteins of barley powdery U., 3CSRS, RIKEN, 4IPSR, Okayama U.) mildew Yaeno, T., C. Inoue, H. Takei, M. Wahara, A. *Presentations for the Chugoku branch meeting of Nakamura, A. Shimizu, T. Toda, K. Kobayashi Japanese Society of Breeding. and N. Yamaoka (Fac. Agr., Ehime U.)

Wheat Inf. Serv. 123: eWIS 123.2, 2017. www.shigen.nig.ac.jp/ewis

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eWIS welcomes manuscripts that provide test (1) Research information: Original research results, technical tips, protocols, mutant and articles in the field of wheat sciences germplasm descriptions, map information, and any The manuscript should start with a title, other information that may be useful in the lab and the names of author(s), affiliation(s), abstract, field. The articles are informal, non-peer-reviewed, followed by the text. Abstract may be omitted thus do not constitute formal publications. Only if not necessary. There is no fixed limit on the manuscripts that require minimal editing will be length but a concise presentation is considered for publication. encouraged. Note well that the data and ideas published in (2) Research Opinion & Topics: Reviews, eWIS are made available by their authors with the minireviews, trends and topics in wheat understanding that they will not be used in research. publications without the authors’ specific consent. Authors who wish to submit a This means that the eWIS articles may not be cited (mini-)review should contact the Editorial without permission from the authors. Copyright is Office prior to submission. retained by the author. (3) Meeting Reports: Announcement of forthcoming meeting and reports on the Manuscript Submission meeting attended Only manuscripts written in English will be (4) Others: Any other information useful for considered for publication. Follow the wheat researchers Instructions strictly. Refer to the latest eWIS articles for the format. Title, Affiliation and Abstract Electronic submission is mandatory. All In the title page(s), the manuscript category (as manuscripts should be submitted using the mentioned above), a title, the names of the online manuscript submission system in the author(s), affiliation(s) and address(es) of the eWIS page (http://shigen.lab.nig.ac.jp/ewis/) authors, and the e-mail address, telephone, and that is linked from the “KOMUGI” page fax numbers of the corresponding author must be (http://www.shigen.nig.ac.jp/wheat/komugi/top/ clearly indicated. top.jsp). The Abstract (100-250 words) may not Editorial Office will inform authors of the contain references. status of their manuscript via e-mail as quickly as possible. The “eWIS online submission system” References offers easy and straightforward web-based References should be cited in the text by the submission procedures. For text writing, author(s) and year, and listed at the end of the text Microsoft Word is recommended. Manuscripts with the names of authors arranged alphabetically. should be double-spaced and page-numbered When an article has more than two authors, only starting from the title page. Do not use line the first author's name should appear, followed by numbers. Figures including illustrations, "et al.", in the text. The references should be photographs and color plates should be submitted formatted as follows. as JPEG files. Tables should be submitted as Journal articles: Microsoft Word file. Supplemental data, such as a Payne PI, Holt LM, Law CN (1981) Structural large table ranging several pages, should be and genetical studies on the high molecular submitted either as Microsoft Excel or Adobe weight subunits of wheat glutenin. Theor PDF file. Appl Genet 60:229-236. Book chapters: Manuscript Categories Peacock WJ, Dennis ES, Gerlach WJ (1981) eWIS accepts the following categories of papers: Molecular aspects of wheat evolution:

repeated DNA sequences. In: Evans LT The DDBJ/EMBL/GenBank accession numbers and Peacock WJ (eds.) Wheat Science - must be provided for newly reported nucleotide Today and Tomorrow. Cambridge Univ. sequences. Press, Cambridge, UK, pp. 41-60. Books: Tables Knott DR (1989) The Wheat Rusts - Breeding Tables must be numbered consecutively. For for Rust Resistance. Springer-Verlag, New Table writing, Microsoft Word is recommended. York, USA. Prepare a separate file for each table. Refer to the latest eWIS articles for format. Articles in preparation or articles submitted for publication, unpublished observations, Figures personal communications, etc. should not be Figures must be numbered consecutively. Prepare included in the reference list but should only be a separate file for each figure. mentioned in the article text (e.g., K. Tsunewaki personal communication). Outline of the publication process Authors of accepted manuscripts are informed by Abbreviations e-mail that a temporary URL has been created Abbreviations should be explained at first from which they can obtain their proof in PDF occurrence. format. Proofreading is the responsibility of the author. Authors should make proof corrections Symbols and Units and send them to Editorial Office by e-mail. After Gene names and protein names must carefully be online publication, corrections can only be made discriminated. Gene names and loci should be in exceptional cases when Editorial Office italicized; protein should be upright. The SI units permits the necessity. (http://physics.nist.gov/Pubs/SP330/contents.html The final version of accepted manuscripts will ) should be used throughout. be published in the ‘Latest Articles’ section of the eWIS web page upon receipt of proof corrections. Nomenclature Editorial Office biannually gathers the accepted Nomenclature of genes and chromosomes should manuscripts published in the ‘Latest Articles’ into follow the ‘Catalogue of gene symbols for wheat’ a volume. In ‘Archive’ of eWIS, all manuscripts (McIntosh et al.: 10th Int. Wheat Genet. Symp. are open to all wheat researchers. 2003). No hard-copy edition will be supplied. For each volume, a PDF edition will be available for Nucleotide sequences downloading.