Online Material Online Material 1
Table. Taxa used in this study with locality data and Genbank accession numbers.
Taxon Abbrev No. Subfamily Tr i b e L o c ality data Opsin Wingless EF1
(~1200bp) (450bp) (~1600bp)
Rophitinae
Conanthalictus wilmattae Cowi 351 Rophitinae Conanthalictini USA: California [J. Neff] AY227934 AY222553 AF435378
Goeletapis peruensis Gope 569 Rophitinae Penapini Peru: Lima Dept. [J.G. Rozen, AY227923* AY222560 AF435386 Jr.] Penapis penai Pnpe 572 Rophitinae Penapini Chile: Huasco Prov. [J.G. AY227921 AY222558 AF435401 Rozen, Jr.] Penapis toroi Pnto 586 Rophitinae Penapini Chile: Region III [L. Packer] AY227922 AY222559 AF435402
Dufourea malacothricis Duma 235 Rophitinae Rophitini USA: Michigan [P. Lincoln] AY227917 AY222554 AF435382
Dufourea mulleri Dumu 233 Rophitinae Rophitini USA: Michigan [P. Lincoln] AY227918 AY222555 AF435383
Dufourea novaeangliae Duno 354 Rophitinae Rophitini USA: New York, Cayuga Co. AY227919 AY222556 AF435384
Sphecodosoma pratti Sspr 355 Rophitinae Rophitini USA: Texas, Mason Co. AY227924 AY222561 AF435410 [J. Neff] Systropha curvicornis Sycu 350 Rophitinae Rophitini Austria: Vienna [M. Ayasse] AY227925 AY222562 AF435411
Systropha planidens Sypl 349 Rophitinae Rophitini Austria: Vienna [M. Ayasse] AY227926 AY222563 AF435412
Protodufourea parca Pfsp 237 Rophitinae Xeralictini USA: Arizona, Pima Co. AY227920 AY222557 AF435399 [L. Packer] Xeralictus bicuspidariae Xrbi 566 Rophitinae Xeralictini USA: California, San Diego Co. AY227927 AY222564 AF435413 [R. Snelling] Xeralictus timberlakei Xrti 564 Rophitinae Xeralictini USA: California, Riverside Co. AY227928 AY222565 AF435414 [R. Snelling]
Nomiinae
Dieunomia nevadensis None 207 Nomiinae USA: Arizona, Cochise Co. AY227931 AY222568 AF435396
Dieunomia triangulifera Notr 286 Nomiinae USA: Kansas, Douglas Co. AY227932 AY222569 AF435397
Lipotriches (Austro.) Noau 754 Nomiinae Australia: South Australia, AY227930 AY222567 AF435395 australica Cowell Pseudapis unidentata Psun 241 Nomiinae Spain: Almeria Prov. AY227933 AY222570 AF435404
Nomioidinae
Cellariella brooksi Cebr 1205 Nomioidinae Madagascar: 8 km N. Tulear, EU203280 EU203218 EU203249 19 Oct. 2004 Nomioides Nosp 1209 Nomioidinae Madagascar: 19 km N. Tulear, EU203281 EU203219 EU203250 (Atronomioides) tumidus 17 Oct. 2004 Nomioides (Nomioides) Nmsp 243 Nomioidinae Spain: Granada Prov. AY227929 AY222566 AF435394 facilis
Halictinae
Augochlorella pomoniella Aupo 592 Halictinae Augochlorini USA: California, Inyo Co. AY227935 AY222572 AF435373 [J. Ascher] Augochloropsis metallica Aume 334/5 Halictinae Augochlorini USA: New York, Tompkins Co. AY227934 AY222571 AF140315
Corynura patagonica Copt 581 Halictinae Augochlorini Chile: Region IX, near Temuco AY227936 AY222573 na [L. Packer] Megalopta genalis Mgge 247 Halictinae Augochlorini Republic of Panama: Panama AY227937 AY222574 AF140316 Prov. [M. Engel] Neocorynura discolor Ncdi 249 Halictinae Augochlorini Colombia [M. Engel] AY227938 AY222575 AF140317
Agapostemon leunculus Agle 336 Halictinae Caenohalictini Republic of Panama: Panama AY227939 AY222576 AF435371 Prov. [W. Wcislo] Agapostemon tyleri Agty 230 Halictinae Caenohalictini USA: Arizona, Cochise Co. AY227940 AY222577 AF140320
Caenohalictus sp. 2 Cnsp 578 Halictinae Caenohalictini Chile: Region I, Socoroma AY227941 AY222578 AF435376 [L. Packer] Caenohalictus sp. 3 Cnsp 788 Halictinae Caenohalictini Brazil: Minas Gerais AY227942 AY222579 AF435377 [D. Yanega] 2 Online Material
Table. Continued
Taxon Abbrev No. Subfamily Tr i b e L o c ality data Opsin Wingless EF1
(~1200bp) (450bp) (~1600bp)
Dinagapostemon sp. 1 Dnsp 573 Halictinae Caenohalictini Republic of Panama: Chiriqui AY227943 AY222580 AF435380 Prov. [L. Packer] Dinagapostemon sp. 2 Dnsp 790 Halictinae Caenohalictini Republic of Panama: Cocle AY227944 AY222581 AF435381 Prov. [L. Packer] Habralictus sp. Hasp 786 Halictinae Caenohalictini Brazil: Minas Gerais AY227945 AY222582 AF435387 [D. Yanega] Pseudagapostemon Psbr 347 Halictinae Caenohalictini Brazil: Minas Gerais AY227946 AY222583 AF140323 braziliensis [D. Yanega] Pseudagapostemon pissisi Psps 576 Halictinae Caenohalictini Chile: Region VIII AY227947 AY222584 AF435403 (nr. Temuco) [L. Packer] Rhinetula denticrus Rhdn 799 Halictinae Caenohalictini Republic of Panama: Panama AY227948 AY222585 AF435405 Prov. [D. Roubik] Ruizantheda mutabilis Rumt 574 Halictinae Caenohalictini Chile: Region VIII AY227949 AY222586 AF435406 (nr. Temuco) [L. Packer] Ruizantheda proxima Rupr 575 Halictinae Caenohalictini Chile: Region VIII AY227950 AY222587 AF435407 (nr. Temuco) [L. Packer] Halictus (Halictus) Haqu 89 Halictinae Halictini France: Dordogne AY227956 AY222592 AF140334 quadricinctus [C. Plateaux-Quenu] Halictus (Seladonia) Hatr 93 Halictinae Halictini USA: Arizona, Cochise Co. AY227957 AY222593 AF140310 tripartitus Halictus (Vestitohalictus) Veve 373 Halictinae Halictini Spain: Granada Prov. AY227958* AY222594 AF140313 vestitus Lasioglossum Auli 711 Halictinae Halictini Australia: Victoria AY227962 AY222598 AF435372 (Australictus) lithuscum Lasioglossum Chfl 320 Halictinae Halictini Australia: South Australia AY227966 AY222602 AF264792 (Chilalictus) florale [S. Reyes] Lasioglossum Chla 316 Halictinae Halictini Australia: Victoria [S. Reyes] AY227967 AY222603 AF264793 (Chilalictus) lanarium Lasioglossum Ctni 1192 Halictinae Halictini Madagascar: Parc National EU203307 EU203248 EU203279 (Ctenonomia) nicolli Isalo Lasioglossum (Dialictus) Dizp 74 Halictinae Halictini USA: New York, Seneca Co. AF448918* AY222607 AF435379 zephyrum Lasioglossum (Evylaeus) Evca 105 Halictinae Halictini France: Dordogne AF448877* AY222608 AF435385 calceatum [C. Plateaux-Quenu] Lasioglossum Helu 186 Halictinae Halictini USA: Texas, Bastrop Co. AF448904* AY222609 AF435388 (Hemihalictus) lustrans [J. Neff] Lasioglossum Homg 360 Halictinae Halictini Australia: Western Australia, AY227964 AY222600 AF264839 (Homalictus) megastigmus Bluff Knoll [S. Reyes] Lasioglossum Hopu 245 Halictinae Halictini Australia: South Australia, AY227965 AY222601 AF435389 (Homalictus) punctatus Adelaide [S. Reyes] Lasioglossum Laat 556 Halictinae Halictini USA: New York, Tompkins AF448867 AY222604 AF435390 (Lasioglossum) Co. athabascense Lasioglossum Lasc 550 Halictinae Halictini Japan: Shimane Prefecture AY227968 AY222605 AF435391 (Lasioglossum) scitulum [R. Miyanaga] Lasioglossum Lazo 284 Halictinae Halictini USA: New York, Tompkins AY227969 AY222606 AF264855 (Lasioglossum) zonulum Co. Lasioglossum Pahy 299 Halictinae Halictini Australia: South Australia AY227963 AY222599 AF264857 (Parasphecodes) [S. Reyes] hybodinum Mexalictus arizonensis Mxaz 98 Halictinae Halictini USA: Arizona, Santa Cruz Co. AY227959 AY222595 AF140322 [L. Packer] Pa tellapis (Archihalictus) Arjo 1188 Halictinae Halictini Madagascar: Parc National EU203301 EU203242 EU203273 joffrei Ranomafana Pa tellapis (Chaetalictus) Chco 1189 Halictinae Halictini Madagascar: Parc National EU203302 EU203243 EU203274 coccinea Ranomafana Pa tellapis (Chaetalictus) Chsp 911 Halictinae Halictini South Africa: Western Cape EU203289 EU203231 EU203261 minima Prov., Citrusdal Pa tellapis (Chaetalictus) Chsp 603 Halictinae Halictini South Africa: Kwazulu-Natal AY227952 AY222588 AF435374 sp. 2 [L. Packer] Pa tellapis (Chaetalictus) Chsp 956 Halictinae Halictini South Africa: Mpumalanga, EU203290 EU203230 EU203262 spec. nov. 66 Graskop Pa tellapis (Lomatalictus) Losp 604 Halictinae Halictini South Africa: Gauteng AY227953 AY222589 AF435393 sp. [C. Eardley] Pa tellapis (Lomatalictus) Lole 954 Halictinae Halictini South Africa: Mpumalanga, EU203291 EU203232 EU203263 malachurinus Graskop Online Material 3
Table. Continued
Taxon Abbrev No. Subfamily Tr i b e L o c ality data Opsin Wingless EF1
(~1200bp) (450bp) (~1600bp)
Pa tellapis (Madagalictus) Mdca 1194 Halictinae Halictini Madagascar: Parc National EU203303 EU203244 EU203275 castaneus Ranomafana Pa tellapis (Madagalictus) Mdin 1195 Halictinae Halictini Madagascar: Parc National EU203304 EU203245 EU203276 inelegans Ranomafana Pa tellapis (Madagalictus) Mdly 1204 Halictinae Halictini Madagascar: Parc National EU203305 EU203246 EU203277 lyalli Ranomafana Pa tellapis (Pachyhalictus) Phsp 357 Halictinae Halictini Vietnam: Ban Don [L. Packer] AY227954 AY222590 AF435400 sp. Pa tellapis (Patellapis) Pabr 1196 Halictinae Halictini South Africa: Western Cape EU203306 EU203247 EU203278 braunsella Prov., Nieuwoudtville Pa tellapis (Patellapis) cf. Pasp1 915 Halictinae Halictini South Africa: Western Cape EU203293 EU203234 EU203265 minutior spec. nov. Prov, Citrusdal Pa tellapis (Patellapis) Pamn 914 Halictinae Halictini South Africa: Western Cape EU203292 EU203233 EU203264 montagui Prov., Clanwilliam Pa tellapis (Patellapis) Pasp2 918 Halictinae Halictini South Africa: Western Cape EU203294 EU203235 EU203266 spec. nov. 10 Prov., Vanrhynsdorp Pa tellapis (Patellapis) Chrv 909 Halictinae Halictini South Africa: Northern Cape EU203288 EU203229 EU203260 spec. nov. 114 Prov., Port Nolloth Pa tellapis (Zonalictus) Zohe 957 Halictinae Halictini South Africa: Mpumalanga, EU203300 EU203241 EU203272 high elevation sp. Sabie Pa tellapis (Zonalictus) Zoab 950 Halictinae Halictini South Africa: Free State, EU203295 EU203236 EU203267 abessinicus Harrismith Pa tellapis (Zonalictus) Zosp 949 Halictinae Halictini South Africa: Free State, EU203297 EU203238 EU203269 albofasciatus Koppies Pa tellapis (Zonalictus) Zoan 919 Halictinae Halictini South Africa: Western Cape EU203296 EU203237 EU203268 andreniformis Prov., Citrusdal Pa tellapis (Zonalictus) Zovt 921 Halictinae Halictini South Africa: Western Cape EU203298 EU203239 EU203270 vittatus Prov., Citrusdal Pa tellapis (Zonalictus) Zoeh 955 Halictinae Halictini South Africa: Mpumalanga: EU203299 EU203240 EU203271 zacephalum Graskop Thrincohalictus Thpr 434 Halictinae Halictini Israel: Golan Heights [L. AY227955 AY222591 AF140326 prognathus Packer] Eupetersia (Eupetersia) Eusy 1243 Halictinae Sphecodini Madagascar: Parc National EU203287 EU203228 EU203259 seyrigi Ranomafana Sphecodes autumnalis Spau 1019 Halictinae Sphecodini NY: Cortland Co., na EU203225 EU203256 Cortlandville [Ascher] Sphecodes clematidis Spcl 1020 Halictinae Sphecodini NY: Tompkins Co., Salmon na EU203226 EU203257 Creek [Ascher] Sphecodes confertus Spcn 1017 Halictinae Sphecodini NY: Tompkins Co., Salmon na EU203227 EU203258 Creek [Ascher] Sphecodes minor Spmi 21 Halictinae Sphecodini USA: New York, Tompkins AY227960 AY222596 AF140324 Co. Sphecodes ranunculi Spra 337 Halictinae Sphecodini Canada: Nova Scotia [L. AY227961 AY222597 AF140325 Packer] Pa rathrinchostoma seyrigi Ptsk 1248 Halictinae Thrinchostomini Madagascar: Parc National EU203287 EU203224 EU203255 Ranomafana [Pauly] Thrinchostoma Trsp 464 Halictinae Thrinchostomini Borneo: Sarawak [S. Heydon] AY227951 na na (Diagonozus) sp. Thrinchostoma Thkd 952 Halictinae Thrinchostomini South Africa: Kwazulu-Natal, EU203282 EU203220 EU203251 (Eothrinchostoma) kandti Hluhluwe Thrinchostoma Thto 953 Halictinae Thrinchostomini South Africa: Kwazulu-Natal, EU203283 EU203221 EU203252 (Eothrinchostoma) Hluhluwe torridum Thrinchostoma Thco 1199 Halictinae Thrinchostomini Madagascar: Parc National EU203284 EU203222 EU203253 (Thrinchostoma) Ranomafana conjugens Thrinchostoma Thle 1200 Halictinae Thrinchostomini Madagascar: Parc National EU203285 EU203223 EU203254 (Thrinchostoma) lemuriae Ranomafana
*=OpsFor/Rev only (others are For/Rev4)