Anti-KPNA1 Monoclonal Antibody, Clone 3B5-2C6 (DCABH-1542) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

Anti-KPNA1 monoclonal antibody, clone 3B5-2C6 (DCABH-1542) This product is for research use only and is not intended for diagnostic use.

PRODUCT INFORMATION

Product Overview Mouse monoclonal to SRP1

Antigen Description Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS.

Immunogen Recombinant full length protein, corresponding to amino acids 1-538 of Human SRP1 with 26kDa proprietary tag (AAH02374).

Isotype IgG1

Source/Host Mouse

Species Reactivity Human

Clone 3B5-2C6

Purity Protein A purified

Conjugate Unconjugated

Applications WB, IHC-P, Sandwich ELISA

Positive Control Human testis tissue; HeLa cell lysate; SRP1 transfected 293T lysate.

Format Liquid

Size 50 μg

Buffer pH: 7.20; Constituent: 99% PBS

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Storage store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Ship Shipped at 4°C.

GENE INFORMATION

Gene Name KPNA1 karyopherin alpha 1 (importin alpha 5) [ Homo sapiens ]

Official Symbol KPNA1

Synonyms KPNA1; karyopherin alpha 1 (importin alpha 5); importin subunit alpha-1; IPOA5; NPI 1; RCH2; SRP1; SRP1-beta; importin alpha 5; importin-alpha-S1; RAG cohort protein 2; nucleoprotein interactor 1; karyopherin subunit alpha-1; recombination activating gene

Entrez Gene ID 3836

Protein Refseq NP_002255

UniProt ID P52294

Chromosome Location 3q21

Pathway Activation of DNA fragmentation factor, organism-specific biosystem; Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis induced DNA fragmentation, organism-specific biosystem; Apoptotic executionphase, organism-specific biosystem; Class I PI3K signaling events mediated by Akt, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;

Function nuclear localization sequence binding; protein binding; protein transporter activity;

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]