Catalog: OM214204 Scan to get more validated information
Irak1 Antibody (Center)
Catalog: OM214204 100ug
Product profile
Product name Irak1 Antibody (Center)
Antibody Type Primary Antibodies
Key Feature
Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications WB ,FC
Species Reactivity Human
Concentration 1 mg/ml
Purification
Target Information
Gene Synonyms Il1rak
Alternative Names Irak1; Il1rak; Interleukin-1 receptor-associated kinase 1; Pelle-like protein kinase
Molecular Weight(MW) 77269 Da
Function Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against forei gn pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MY D88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 pho sphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 u biquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination o
f IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing to gether the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/T AK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activati
on. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation Phosp
horylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleu s, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When s
umoylated, translocates to the nucleus and phosphorylates STAT3 (By similarity)
Tissue Specificity Highly expressed in liver, followed by kidney and skeletal muscle
Cellular Localization Cytoplasm (By similarity). Nucleus (By similarity). Lipid droplet. Note=Translocates to the nucleus when su moylated (By similarity). RSAD2/viperin recruits it to the lipid droplet
Database Links
Entrez Gene 16179
Nucleotide Accession NP_001171447.1;NP_032389.2
Application
Application Mouse Irak1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Irak1 antibody detected the Irak1 protein (arrow).
Application Mouse Irak1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application Notes WB~~1:100~500 FC~~1:10~50:
Additional Information
Form Liquid
Storage Instructions For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified thro ugh a protein A column, followed by peptide affinity purification.
Note The product is for research use only,not for use in diagnostic or therapeutic procedures. OmnimAbs.com [email protected] 506 N. GARFIELD AVE # 210 ALHAMBRA, CA 91801 This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt