DOCSLIB.ORG

New Trisulfated Steroids from the Vietnamese Marine Sponge Halichondria Vansoesti and Their PSA Expression and Glucose Uptake Inhibitory Activities

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
New Trisulfated Steroids from the Vietnamese Marine Sponge Halichondria Vansoesti and Their PSA Expression and Glucose Uptake Inhibitory Activities marine drugs Article New Trisulfated Steroids from the Vietnamese Marine Sponge Halichondria vansoesti and Their PSA Expression and Glucose Uptake Inhibitory Activities Kseniya M. Tabakmakher 1, Tatyana N. Makarieva 1,*, Vladimir A. Denisenko 1, Roman S. Popov 1, Pavel S. Dmitrenok 1 , Sergey A. Dyshlovoy 1,2 , Boris B. Grebnev 1, Carsten Bokemeyer 2, Gunhild von Amsberg 2,3 and Nguyen X. Cuong 4 1 G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Pr. 100-let Vladivostoku 159, 690022 Vladivostok, Russia 2 Department of Oncology, Hematology and Bone Marrow Transplantation with Section Pneumology, Hubertus Wald-Tumorzentrum, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany 3 Martini-Klinik Prostate Cancer Center, University Hospital Hamburg-Eppendorf, 20251 Hamburg, Germany 4 Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), Hanoi 100000, Vietnam * Correspondence: [email protected]; Tel.: +7-950-295-6625 Received: 26 June 2019; Accepted: 24 July 2019; Published: 27 July 2019 Abstract: Seven new unusual polysulfated steroids—topsentiasterol sulfate G (1), topsentiasterol sulfate I (2), topsentiasterol sulfate H (3), bromotopsentiasterol sulfate D (4), dichlorotopsentiasterol sulfate D (8), bromochlorotopsentiasterol sulfate D (9), and 4β-hydroxyhalistanol sulfate C (10), as well as three previously described—topsentiasterol sulfate D (7), chlorotopsentiasterol sulfate D (5) and iodotopsentiasterol sulfate D (6) have been isolated from the marine sponge Halichondria vansoesti. Structures of these compounds were determined by detailed analysis of 1D- and 2D-NMR and HRESIMS data, as well as chemical transformations. The effects of the compounds on human prostate cancer cells PC-3 and 22Rv1 were investigated. Keywords: marine sponge; Halichondria vansoesti; trisulfated steroids; topsentiasterol sulfates; halistanol sulfates; anticancer activity; PSA expression; glucose uptake 1. Introduction Biologically active trisulfated steroids are characteristic secondary metabolites found in some marine sponges. These polar steroids comprise several structural subgroups in the sponges. The first compound, bearing a common 2β,3α,6α-trisulfoxy steroid nucleus, halistanol sulfate, was isolated in 1981 from the Okinawan sponge Halichondria cf. moorei [1] (Figure S1). The subgroup also includes sokotrasterol sulfate from the sponge Halichondria sp. [2], halistanol sulfates A-J and polasterol B, found in the sponges Epipolasis sp. [3,4], Pseudoaxinissa digitata [5], and Halichondria sp. [6], ophirapsranol trisulfate from Topsentiaophiraphidites [7], four sterols isolated from the sponges Trachyopsis halichondroides and Cymbastela coralliophila [8], amaranzoles A-F from Phorbas amaranthus [9,10], and topsentinol K trisulfate from the sponge Topsentia sp. [11]. Another subgroup of these metabolites consists of ibisterol sulfates and lembesterol A from the sponges Topsentia sp. [12], Xestospongia sp. [13], and Petrosia strongilata [14]. In their steroid nuclei, 2β,3α,6α-trisulfoxy functionality is combined with a C-9(11)-double bond and a methyl group at C-14. One more subgroup includes topsentiasterol sulfates A–E from the sponge Topsentia sp. [15], Sch 575867 from the deep-water sponge belonging to the family Astroscleridae [16], spheciosterol sulfates from the sponge Spheciospongia sp. [17], as well as chloro- and iodotopsentiasterol sulfates D, isolated from the sponge Topsentia sp. in our laboratory [18]. Mar. Drugs 2019, 17, 445; doi:10.3390/md17080445 www.mdpi.com/journal/marinedrugs Mar. Drugs 2019, 17, x 2 of 15 functionality is combined with a C-9(11)-double bond and a methyl group at C-14. One more subgroup includes topsentiasterol sulfates A–E from the sponge Topsentia sp. [15], Sch 575867 from the deep-water sponge belonging to the family Astroscleridae [16], spheciosterol sulfates from the Mar.sponge Drugs Spheciospongia2019, 17, 445 sp. [17], as well as chloro- and iodotopsentiasterol sulfates D, isolated from2 of the 14 sponge Topsentia sp. in our laboratory [18]. These compounds contain a common Δ9(11)-unsaturated, 4β-hydroxy-14α-methyl, 2β,3α,6α-trisulfated steroid nucleus. TheseIn compounds addition to contain unusual a common structuralD9(11) features,-unsaturated, trisulfated 4β-hydroxy-14 steroids αpossess-methyl, promising 2β,3α,6α-trisulfated biological steroidproperties nucleus. [19]. In fact, a broad range of activities has been described to trisulfate steroids such as antibacterialInadditiontounusualstructuralfeatures, [1,15,20], antifungal [15,16,21], trisulfatedsteroidspossesspromisingbiologicalproperties[ antiviral (including anti-HIV and anti-HSV effects)19]. In[5,12,13,22,23], fact, a broad rangeantiparasitic of activities [21], and has beenantiplatelet described activities to trisulfate [24]. In steroids addition, such the as inhibition antibacterial of different [1,15,20], antifungalenzymes [6,11,18], [15,16,21 ],promotion antiviral (including of angiogenesis anti-HIV [25], and and anti-HSV antitumoreffects) activity [5,12 against,13,22,23 various], antiparasitic tumor [cell21], andlines antiplatelet [7,15,17,26] activities have been [24 ].described. In addition, Thus, the the inhibition search offor different new trisulfated enzymes steroids [6,11,18], from promotion sponges, of angiogenesisincluding the [25 analyses], and antitumor of their activitychemical against structures various and tumor physiological cell lines [7 properties,,15,17,26] have continues been described. to be a Thus,promising the search area forof research. new trisulfated Hopefully, steroids this from may sponges, lead to including the development the analyses of a of new their generation chemical structures of drugs andfor a physiological broad spectrum properties, of diseases. continues to be a promising area of research. Hopefully, this may lead to the developmentIn the course of a new of our generation ongoing of interest drugs for in anew broad biologically spectrum active of diseases. secondary metabolites of marine invertebrates,In the course Halichondria of our ongoing vansoesti interest sponge, in newcollected biologically in Vietnamese active secondary waters during metabolites the 49th of scientific marine invertebrates,cruise aboard Halichondriathe R/V ‘Academic vansoesti Oparin’,sponge, was collected investigated. in Vietnamese As a result, waters ten during trisulfated the 49th steroids scientific 1–10 cruisewere isolated aboard the(Figure R/V ‘Academic1). Using NMR Oparin’, spectroscopy, was investigated. including As a1H, result, 13C, tenHSQC, trisulfated COSY, steroidsHMBC,1 and–10 wereNOESY, isolated as well (Figure as high-resolution1). Using NMR mass spectroscopy, spectrometry including and chemical1H, 13 C,transformations, HSQC, COSY, 1 HMBC,–4 and 8 and–10 NOESY,were identified as well as as high-resolution new, unusual mass analogues spectrometry of to andpsentiasterol chemical transformations,sulfates and halistanol1–4 and 8 –sulfates.10 were identifiedCompounds as new,5–7 unusualwere previously analogues known of topsentiasterol as chlorotopsen sulfatestiasterol and halistanol sulfate D, sulfates. iodotopsentiasterol Compounds 5sulfates–7 were D previously [18], and known topsentiasterol as chlorotopsentiasterol sulfate D, respectively sulfate D, iodotopsentiasterol[15]. Herein, we report sulfates the D isolation, [18], and topsentiasterolstructural elucidation, sulfate proposed D, respectively biosynthetic [15]. Herein, pathwa weys, reportand the the study isolation, of the structuralbiological elucidation,activities of proposedthe isolated biosynthetic compounds. pathways, and the study of the biological activities of the isolated compounds. 29 1:R=a 21 22 2:R=b 26 20 20 24 18 R 3:R=c 25 17 11 H 3a:R=c* 11 17 H 27 19 13 4:R=d H 1 H 1 9 14 9 14 NaO3SO 5:R=e NaO3SO 15 10 6:R=f 10 10 3 5 30 H H 7 1-9 NaO SO 7:R=g NaO SO 3 H 8:R=h 3 H HO OSO3Na 9:R=i HO OSO3Na O O O O 26 Br 25 25 26 26 26 25 25 25 26 NH OH OCH3 27 O 28 27 27 27 O 28 31 28 OH 28 OCH3 27 a b 32 28 O cdc* O O Cl I Cl Cl 26 25 26 25 26 25 26 25 26 25 O O O O 27 27 O 27 27 27 28 28 28 28 e g 28 f h i Br Cl FigureFigure 1.1. TheThe structuresstructures ofof 11−10. − 2. Results and Discussion 2. Results and Discussion Concentrated EtOH extract of the sponge Halichondria vansoesti was partitioned between aqueous Concentrated EtOH extract of the sponge Halichondria vansoesti was partitioned between EtOH and n-hexane. The aqueous EtOH-soluble materials were further applied on a reversed-phase aqueous EtOH and n-hexane. The aqueous EtOH-soluble materials were further applied on a column chromatography (YMC-gel) and eluted successively with H2O EtOH:H2O (3:7) EtOH:H2O reversed-phase column chromatography (YMC-gel) and eluted successively! with H2O!→EtOH:H2O (2:3) EtOH:H2O (1:1) EtOH:H2O (3:2) resulting in several subfractions. Subfractions obtained by (3:7)!→EtOH:H2O (2:3)!→EtOH:H2O (1:1)→EtOH:H2O (3:2) resulting in several subfractions. elution with EtOH:H2O (3:7) to EtOH:H2O (6:4) were further purified by a reversed-phase HPLC (YMC-ODS-A) to give 1, 2 and 4–10. The subfraction, eluted with H2O, was further extracted with BuOH, after which the butanol solution was concentrated and subjected to a reversed-phase HPLC (YMC-ODS-A) to obtain 3. Mar. Drugs 2019, 17, 445 3 of 14 The molecular formula of 1,C H NNa O S , was established from the [M Na] ion peak 30 44 3 14 3 3Na − − at m/z 784.1724 in the ( )-HRESIMS. In addition, the peaks at m/z 380.5927
Load more

Recommended publications
  • Basal Metazoans - Dirk Erpenbeck, Simion Paul, Michael Manuel, Paulyn Cartwright, Oliver Voigt and Gert Worheide
    EVOLUTION OF PHYLOGENETIC TREE OF LIFE - Basal Metazoans - Dirk Erpenbeck, Simion Paul, Michael Manuel, Paulyn Cartwright, Oliver Voigt and Gert Worheide BASAL METAZOANS Dirk Erpenbeck Ludwig-Maximilians Universität München, Germany Simion Paul and Michaël Manuel Université Pierre et Marie Curie in Paris, France. Paulyn Cartwright University of Kansas USA. Oliver Voigt and Gert Wörheide Ludwig-Maximilians Universität München, Germany Keywords: Metazoa, Porifera, sponges, Placozoa, Cnidaria, anthozoans, jellyfishes, Ctenophora, comb jellies Contents 1. Introduction on ―Basal Metazoans‖ 2. Phylogenetic relationships among non-bilaterian Metazoa 3. Porifera (Sponges) 4. Placozoa 5. Ctenophora (Comb-jellies) 6. Cnidaria 7. Cultural impact and relevance to human welfare Glossary Bibliography Biographical Sketch Summary Basal metazoans comprise the four non-bilaterian animal phyla Porifera (sponges), Cnidaria (anthozoans and jellyfishes), Placozoa (Trichoplax) and Ctenophora (comb jellies). The phylogenetic position of these taxa in the animal tree is pivotal for our understanding of the last common metazoan ancestor and the character evolution all Metazoa,UNESCO-EOLSS but is much debated. Morphological, evolutionary, internal and external phylogenetic aspects of the four phyla are highlighted and discussed. SAMPLE CHAPTERS 1. Introduction on “Basal Metazoans” In many textbooks the term ―lower metazoans‖ still refers to an undefined assemblage of invertebrate phyla, whose phylogenetic relationships were rather undefined. This assemblage may contain both bilaterian and non-bilaterian taxa. Currently, ―Basal Metazoa‖ refers to non-bilaterian animals only, four phyla that lack obvious bilateral symmetry, Porifera, Placozoa, Cnidaria and Ctenophora. ©Encyclopedia of Life Support Systems (EOLSS) EVOLUTION OF PHYLOGENETIC TREE OF LIFE - Basal Metazoans - Dirk Erpenbeck, Simion Paul, Michael Manuel, Paulyn Cartwright, Oliver Voigt and Gert Worheide These four phyla have classically been known as ―diploblastic‖ Metazoa.
    [Show full text]
  • Mass Mortality Event of White Sea Sponges As the Result of High Temperature in Summer 2018 Alexander Ereskovsky, Dmitry Ozerov, Anatoly Pantyulin, Alexander Tzetlin
    Mass mortality event of White Sea sponges as the result of high temperature in summer 2018 Alexander Ereskovsky, Dmitry Ozerov, Anatoly Pantyulin, Alexander Tzetlin To cite this version: Alexander Ereskovsky, Dmitry Ozerov, Anatoly Pantyulin, Alexander Tzetlin. Mass mortality event of White Sea sponges as the result of high temperature in summer 2018. Polar Biology, Springer Verlag, 2019, 42 (12), pp.2313-2318. 10.1007/s00300-019-02606-0. hal-02379769 HAL Id: hal-02379769 https://hal.archives-ouvertes.fr/hal-02379769 Submitted on 13 Jan 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. 1 Mass mortality event of White Sea sponges as the result of high temperature in 2 summer 2018 3 4 Alexander Ereskovsky,1,2,3* Dmitry A. Ozerov4, Anatoly N. Pantyulin,5 Alexander B. 5 Tzetlin4. 6 7 1 Mediterranean Institute of Marine and Terrestrial Biodiversity and Ecology (IMBE), Aix 8 Marseille University, CNRS, IRD, Avignon Université, Station marine d'Endoume, rue de la 9 Batterie des Lions, 13007 Marseille France 10 2 St. Petersburg State University, Biological Faculty, St. Petersburg, 199034, Russia 11 3 Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Russia, 12 Moscow 13 4 The White Sea Biological Station, M.V.
    [Show full text]
  • Morphological Description of Six Species of Suberitida (Porifera
    Morphological description of six species of Suberitida (Porifera: Demospongiae) from the unexplored north-eastern coast of Brazil, with emphasis on two new species Humberto Fortunato, Thierry Perez, Gisele Lobo-Hajdu To cite this version: Humberto Fortunato, Thierry Perez, Gisele Lobo-Hajdu. Morphological description of six species of Suberitida (Porifera: Demospongiae) from the unexplored north-eastern coast of Brazil, with emphasis on two new species. Journal of the Marine Biological Association of the UK, Cambridge University Press (CUP), 2020, 100 (3), pp.389-400. 10.1017/S0025315420000296. hal-02548881 HAL Id: hal-02548881 https://hal-amu.archives-ouvertes.fr/hal-02548881 Submitted on 7 Dec 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Original Paper Running Head: Suberitida from the unexplored Northeastern Brazil Morphological description of six species of Suberitida (Porifera: Demospongiae) from the unexplored Northeastern Brazil, with emphasis on two new species Humberto F. M. Fortunato1,2, Thierry Pérez3, Gisele Lôbo-Hajdu1,2 1Programa de Pós–Graduação de Oceanografia, Universidade do Estado do Rio de Janeiro, Rua São Francisco Xavier, 524, sala 4018, Bloco E, 20550–013 Maracanã, Rio de Janeiro, Brasil; 2Departamento de Genética, Universidade do Estado do Rio de Janeiro, 524, Rua São Francisco Xavier, 524, PHLC, Sala 205, 20550–013, Maracanã, Rio de Janeiro, Brasil and 3Institut Méditerranéen de Biodiversité et d'Ecologie marine et continentale, CNRS, Aix Marseille Université, IRD, Avignon Université.
    [Show full text]
  • Host-Microbe Symbiosis in the Marine Sponge Halichondria Panicea
    Host-microbe symbiosis in the marine sponge Halichondria panicea Stephen Knobloch Faculty of Life and Environmental Sciences University of Iceland 2019 Host-microbe symbiosis in the marine sponge Halichondria panicea Stephen Knobloch Dissertation submitted in partial fulfilment of a Philosophiae Doctor degree in Biology PhD Committee Viggó Þór Marteinsson Ragnar Jóhannsson Eva Benediktsdóttir Opponents Detmer Sipkema Ólafur Sigmar Andrésson Faculty of Life and Environmental Sciences School of Engineering and Natural Sciences University of Iceland Reykjavik, May 2019 Host-microbe symbiosis in the marine sponge Halichondria panicea Bacterial symbiosis in H. panicea Dissertation submitted in partial fulfilment of a Philosophiae Doctor degree in Biology Copyright © 2019 Stephen Knobloch All rights reserved Faculty of Life and Environmental Sciences School of Engineering and Natural Sciences University of Iceland Askja, Sturlugata 7 101, Reykjavik Iceland Telephone: +354 525 4000 Bibliographic information: Stephen Knobloch, 2019, Host-microbe symbiosis in the marine sponge Halichondria panicea, PhD dissertation, Faculty of Life and Environmental Sciences, University of Iceland, 204 pp. ISBN: 978-9935-9438-8-0 Author ORCID: 0000-0002-0930-8592 Printing: Háskólaprent Reykjavik, Iceland, May 2019 Abstract Sponges (phylum Porifera) are considered one of the oldest extant lineages of the animal kingdom. Their close association with microorganism makes them suitable for studying early animal-microbe symbiosis and expanding our understanding of host-microbe interactions through conserved mechanisms. Moreover, many sponges and sponge- associated microorganisms produce bioactive compounds, making them highly relevant for the biotechnology and pharmaceutical industry. So far, however, it has not been possible to grow biotechnologically relevant sponge species or isolate their obligate microbial symbionts for these purposes.
    [Show full text]
  • Histone Deacetylase Inhibitors from Marine Invertebrates
    biology Review Histone Deacetylase Inhibitors from Marine Invertebrates Claudio Luparello * , Manuela Mauro, Vincenzo Arizza and Mirella Vazzana Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, 90128 Palermo, Italy; [email protected] (M.M.); [email protected] (V.A.); [email protected] (M.V.) * Correspondence: [email protected]; Tel.: +39-91-238-97405 Received: 15 October 2020; Accepted: 25 November 2020; Published: 28 November 2020 Simple Summary: Histone deacetylases (HDACs) are enzymes that control gene expression and are involved in the onset of serious human pathologies, including cancer; hence, their inhibitors (HDACis) have received increased attention in recent years. It is known that marine invertebrates produce significant amounts of molecules showing active pharmacological properties and an extensive spectrum of biomedical applications. This review is focused on the description of the molecular, biochemical, and, where available, physiological aspects of marine invertebrate-derived compounds that possess HDACi properties, taking into consideration their possible utilization as treatment agents against different pathological states. Abstract: Histone deacetylases (HDACs) are key components of the epigenetic machinery controlling gene expression. They are involved in chromatin remodeling events via post-translational histone modifications but may also act on nonhistone proteins, influencing many fundamental cellular processes. Due to
    [Show full text]
  • Osculum Dynamics and Filtration Activity in Small Single-Osculum Explants of the Demosponge Halichondria Panicea
    Vol. 572: 117–128, 2017 MARINE ECOLOGY PROGRESS SERIES Published May 31 https://doi.org/10.3354/meps12155 Mar Ecol Prog Ser OPEN ACCESS Osculum dynamics and filtration activity in small single-osculum explants of the demosponge Halichondria panicea Lars Kumala1,2,3,*, Hans Ulrik Riisgård1, Donald Eugene Canfield4 1Marine Biological Research Centre, University of Southern Denmark, 5300 Kerteminde, Denmark 2Max-Planck Odense Center on the Biodemography of Aging and Department of Biology, University of Southern Denmark, 5230 Odense M, Denmark 3Max Planck Institute for Demographic Research, 18057 Rostock, Germany 4Nordic Center for Earth Evolution, Department of Biology, University of Southern Denmark, 5230 Odense M, Denmark ABSTRACT: Contraction−inflation behavior, including the closure and opening of the exhalant opening (osculum), is common among sponges. This behavior may temporally affect filtration activity, making it difficult to study and understand sponge feeding biology. To examine the inter- play between osculum dynamics and filtration activity, small (18 mm3) single-osculum explants of the demosponge Halichondria panicea were studied. Time-lapse video stereo-microscope record- ings of the osculum cross-sectional area (OSA) were made simultaneously with measurements of the filtration rate (~15°C, ~20 PSU) using the clearance method. Osculum dynamics, as expressed by temporal variation of the OSA, including osculum contraction and expansion, correlated with variability in the explant filtration rate, and no water pumping was observed during periods of osculum closure. A linear relationship between filtration rate (FR) and OSA revealed a constant −1 exhalant jet velocity: vjet = FR/OSA = 2.3 ± CI95% 0.13 cm s . The mean filtration rate of explants −1 −1 was 0.28 ± CI95% 0.06 ml min , corresponding to a volume-specific filtration rate of 15 ml min (cm3 sponge)−1, which is 2 to 3 times higher than that reported for larger individuals of H.
    [Show full text]
  • Advances in Sponge Science
    CHAPTER FOUR Ecological Interactions and the Distribution, Abundance, and Diversity of Sponges Janie Wulff1 Contents 1. Introduction 274 1.1. Evaluating distribution, abundance, and diversity of sponges 277 2. Influences of Abiotic Factors and Ecological Interactions on Sponges in Various Habitats 281 2.1. Subtidal rocky substrata—walls, plateaus, canyons 282 2.2. Subtidal rocky substrata—cobbles and caves 290 2.3. Coral reefs 292 2.4. Coral reefs—cryptic spaces 315 2.5. Mangroves 317 2.6. Sediment dominated habitats, including seagrass meadows 322 2.7. Intertidal shores 325 2.8. Antarctic hard bottoms 327 3. Conclusions 329 Acknowledgements 331 References 331 Abstract Although abiotic factors may be important first-order filters dictating which sponge species can thrive at a particular site, ecological interactions can play substantial roles influencing distribution and abundance, and thus diversity. Ecological interactions can modify the influences of abiotic factors both by further constraining distribution and abundance due to competitive or preda- tory interactions and by expanding habitat distribution or abundance due to beneficial interactions that ameliorate otherwise limiting circumstances. It is likely that the importance of ecological interactions has been greatly Department of Biological Science, Florida State University, Tallahassee, FL, USA 1Corresponding author: Email: [email protected] Advances in Marine Biology, Volume 61 # 2012 Elsevier Ltd ISSN 0065-2881, DOI: 10.1016/B978-0-12-387787-1.00003-9 All rights reserved. 273 274 Janie Wulff underestimated because they tend to only be revealed by experiments and time-series observations in the field. Experiments have revealed opportunistic predation to be a primary enfor- cer of sponge distribution boundaries that coincide with habitat boundaries in several systems.
    [Show full text]
  • Marlin Marine Information Network Information on the Species and Habitats Around the Coasts and Sea of the British Isles
    MarLIN Marine Information Network Information on the species and habitats around the coasts and sea of the British Isles Crustose sponges on extremely wave-surged infralittoral cave or gully walls MarLIN – Marine Life Information Network Marine Evidence–based Sensitivity Assessment (MarESA) Review John Readman 2016-07-07 A report from: The Marine Life Information Network, Marine Biological Association of the United Kingdom. Please note. This MarESA report is a dated version of the online review. Please refer to the website for the most up-to-date version [https://www.marlin.ac.uk/habitats/detail/158]. All terms and the MarESA methodology are outlined on the website (https://www.marlin.ac.uk) This review can be cited as: Readman, J.A.J., 2016. Crustose sponges on extremely wave-surged infralittoral cave or gully walls. In Tyler-Walters H. and Hiscock K. (eds) Marine Life Information Network: Biology and Sensitivity Key Information Reviews, [on-line]. Plymouth: Marine Biological Association of the United Kingdom. DOI https://dx.doi.org/10.17031/marlinhab.158.1 The information (TEXT ONLY) provided by the Marine Life Information Network (MarLIN) is licensed under a Creative Commons Attribution-Non-Commercial-Share Alike 2.0 UK: England & Wales License. Note that images and other media featured on this page are each governed by their own terms and conditions and they may or may not be available for reuse. Permissions beyond the scope of this license are available here. Based on a work at www.marlin.ac.uk (page left blank) Date: 2016-07-07
    [Show full text]
  • Sponge Community of the Western Black Sea Shallow Water Caves: Diversity and Spatial Distribution
    Sponge community of the western Black Sea shallow water caves: diversity and spatial distribution Alexander Ereskovsky1,2, Oleg A. Kovtun3, Konstantin K. Pronin4, Apostol Apostolov5, Dirk Erpenbeck6 and Viatcheslav Ivanenko7 1 Institut Méditerranéen de Biodiversité et d'Ecologie Marine et Continentale (IMBE), Aix Marseille University, CNRS, IRD, Avignon Université, Marseille, France 2 Department of Embryology, Faculty of Biology, Saint-Petersburg State University, Saint-Petersburg, Russia 3 Hydrobiology and General Ecology Department, Marine Research Station, Odessa National I. I. Mechnikov University, Odessa, Ukraine 4 Physical and Marine Geology Department, Odessa National I. I. Mechnikov University, Odessa, Ukraine 5 Burgas University, Burgas, Bulgaria 6 Department of Earth and Environmental Sciences & GeoBio-Center, Ludwig-Maximilians-Universität München, Munich, Germany 7 Department of Invertebrate Zoology, Biological Faculty, Moscow State University, Moscow, Russia ABSTRACT Marine caves possess unique biocoenotic and ecological characteristics. Sessile benthic species such as sponges associated with cave habitats typically show a marked zonation from the cave entrance towards the end of the cave. We describe three semi-submerged karstic caves of 50 to 83 m length and 936 to 2,291 m3 volume from the poorly explored cavernicolous fauna of North-East Bulgaria. We surveyed sponge diversity and spatial variability. Eight demosponge species were identified based on morphological and molecular data, of which six are known from the adjacent open sea waters of the Black Sea. Two species, Protosuberites denhartogi van Soest & de Kluijver, 2003 and Halichondria bowerbanki Burton, 1930, are reported from the Black Sea for the first time. The spatial sponge distribution inside the caves is in general similar, but shows some differences in species composition and distribution depending on cave relief and Submitted 22 December 2017 hydrodynamics.
    [Show full text]
  • Phylum Porifera
    www.nature.com/scientificreports OPEN Development of a multilocus- based approach for sponge (phylum Porifera) identification: refinement Received: 01 September 2016 Accepted: 19 December 2016 and limitations Published: 02 February 2017 Qi Yang1,2, Christopher M. M. Franco1,2, Shirley J. Sorokin1,2,3 & Wei Zhang1,2,4 For sponges (phylum Porifera), there is no reliable molecular protocol available for species identification. To address this gap, we developed a multilocus-based Sponge Identification Protocol (SIP) validated by a sample of 37 sponge species belonging to 10 orders from South Australia. The universal barcode COI mtDNA, 28S rRNA gene (D3–D5), and the nuclear ITS1-5.8S-ITS2 region were evaluated for their suitability and capacity for sponge identification. The highest Bit Score was applied to infer the identity. The reliability of SIP was validated by phylogenetic analysis. The 28S rRNA gene and COI mtDNA performed better than the ITS region in classifying sponges at various taxonomic levels. A major limitation is that the databases are not well populated and possess low diversity, making it difficult to conduct the molecular identification protocol. The identification is also impacted by the accuracy of the morphological classification of the sponges whose sequences have been submitted to the database. Re-examination of the morphological identification further demonstrated and improved the reliability of sponge identification by SIP. Integrated with morphological identification, the multilocus-based SIP offers an improved protocol for more reliable and effective sponge identification, by coupling the accuracy of different DNA markers. Sponges (phylum Porifera), the evolutionary oldest multicellular animals, are sessile, benthic filter-feeders1.
    [Show full text]
  • Diversity, Structure and Convergent Evolution of the Global Sponge Microbiome
    ARTICLE Received 1 Nov 2015 | Accepted 9 May 2016 | Published 16 Jun 2016 DOI: 10.1038/ncomms11870 OPEN Diversity, structure and convergent evolution of the global sponge microbiome Torsten Thomas1, Lucas Moitinho-Silva1, Miguel Lurgi2, Johannes R. Bjo¨rk3,4, Cole Easson5, Carmen Astudillo-Garcı´a6, Julie B. Olson7, Patrick M. Erwin8, Susanna Lo´pez-Legentil8, Heidi Luter9, Andia Chaves-Fonnegra10, Rodrigo Costa11, Peter J. Schupp12, Laura Steindler13, Dirk Erpenbeck14, Jack Gilbert15,16, Rob Knight17, Gail Ackermann17, Jose Victor Lopez10, Michael W. Taylor6, Robert W. Thacker18, Jose M. Montoya3, Ute Hentschel19 & Nicole S. Webster20 Sponges (phylum Porifera) are early-diverging metazoa renowned for establishing complex microbial symbioses. Here we present a global Porifera microbiome survey, set out to establish the ecological and evolutionary drivers of these host–microbe interactions. We show that sponges are a reservoir of exceptional microbial diversity and major contributors to the total microbial diversity of the world’s oceans. Little commonality in species composition or structure is evident across the phylum, although symbiont communities are characterized by specialists and generalists rather than opportunists. Core sponge microbiomes are stable and characterized by generalist symbionts exhibiting amensal and/or commensal interactions. Symbionts that are phylogenetically unique to sponges do not disproportionally contribute to the core microbiome, and host phylogeny impacts complexity rather than composition of the symbiont community. Our findings support a model of independent assembly and evolution in symbiont communities across the entire host phylum, with convergent forces resulting in analogous community organization and interactions. 1 School of Biological, Earth and Environmental Sciences, Centre for Marine Bio-Innovation and School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales 2052, Australia.
    [Show full text]
  • A New Record of Genus Halichondria (Demospongiae: Halichondrida: Halichondriidae) from Korea
    Korean J. Syst. Zool. Vol. 25, No. 1: 137-139, March 2009 A New Record of Genus Halichondria (Demospongiae: Halichondrida: Halichondriidae) from Korea Yong Jin Jeon and Chung Ja Sim* Department of Biological Engineering, College of Life Sciences and Nano Technology, Hannam University, Daejeon 305-811, Korea ABSTRACT Marine sponges were collected from Tongyeong yachtschool harbor, Korea in 2008. Among them Halichon- dria (Halichondria) bowerbanki Burton, 1930 is newly recorded in the Korean fauna. Key words: Halichondriidae, Halichondria, Korea INTRODUCTION Table 1. Comparison in spicule dimension of H.(H.) bower- banki Species Tongyeong The genus Halichondria is characterized by ectosomal ske- H. (H.) bowerbanki Spicule (μm) specimen leton which consists of thin, tangential, clearly detachable Oxeas 200-330×3-12 170-380×2-10 membrane, containing single oxeas or vague bundles of oxeote spicules and it supported by choanosomal columns of oxeas traversing subdermal spaces (Hooper, 1994). Me- 2002, p. 79. gascleres exclusively oxeas or derivates are in a wide size range. About 110 species are distributed worldwide (Hoop- Material examined. Yachtschool harbor, Tongyeong-si, er and van Soest, 2002). Seven species of Halichondria Gyeongsangnam-do, 14 Nov. 2008. have been reported from Korean waters (Kim et al., 1968; Description. Encrusting, attached to substrate with irregular Rho and Lee, 1976; Kang and Sim, 2008a, b). The specimens branches which shaped like tassel reaching 2-6 cm high, were collected by hand from Tongyoung, Korea in 2008. All solid and gets thiner to the end. Oscules opened at the ends procedures were followed the methods of Kang and Sim of each branch with 2-5 mm in diameter.
    [Show full text]