Monoclonal Antibody to VAMP-7 / SYBL1 - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

AM10220PU-N Monoclonal Antibody to VAMP-7 / SYBL1 - Purified

Alternate names: Synaptobrevin-like protein 1, Tetanus-insensitive VAMP, Ti-VAMP, VAMP7, Vesicle- associated membrane protein 7 Quantity: 0.1 mg Concentration: 1.0 mg/ml after reconstitution Background: SYBL1 (Synaptobrevin-like 1) is found ubiquitously and is an unusual v-SNAREs molecule implicated in cellular exocytosis. The SYBL1 gene is found on the pseudoautosomal region (PAR) of X chromosome. It is a highly conserved gene and has been shown to be silenced on inactive X and Y chromosomes. In ICF syndrome, a human disease affecting DNA methylation, SYBL1 escapes from silencing and this correlates with the observed altered patterns of histone methylation and acetylation found. An association between some SYBL1 alleles and bipolar affective disorder (BPAD) has been observed. Uniprot ID: P51809 NCBI: NP_001138621.1 GeneID: 6845 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), AM03095PU-N Controls: Clone: 158.2 Immunogen: GST-TI-VAMP recombinant protein Format: State: Lyophilized purified powder Purification: Affinity Chromatography on Protein A Buffer System: 0.1M Tris, 0.1M Glycine, 2% Sucrose Preservatives: None Reconstitution: Restore in distilled water. Applications: ELISA. Western Blot: 1/500-1/2000. Immunofluorescence. Immunohistochemistry: 1/10-1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Reacts with Human 25 kDa TI-VAMP protein. Cross reacts with TI-VAMP from different species (Mouse, Rat, Canine). Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20151113 1 / 2 AM10220PU-N: Monoclonal Antibody to VAMP-7 / SYBL1 - Purified

General Readings: 1. Danglot L, Chaineau M, Dahan M, Gendron MC, Boggetto N, Perez F, et al. Role of TI- VAMP and CD82 in EGFR cell-surface dynamics and signaling. J Cell Sci. 2010 Mar 1;123(Pt 5):723-35. doi: 10.1242/jcs.062497. Epub 2010 Feb 9. PubMed PMID: 20144992. 2. Muzerelle A, Alberts P, Martinez-Arca S, Jeannequin O, Lafaye P, Mazié JC, et al. Tetanus neurotoxin-insensitive vesicle-associated membrane protein localizes to a presynaptic membrane compartment in selected terminal subsets of the rat brain. Neuroscience. 2003;122(1):59-75. PubMed PMID: 14596849. Pictures: Immunohistochemical satining of Spleen Paraffin Sections using Ti-VAMP antibody Cat.-No AM10220PU-N

Immunofluorescence using Ti-VAMP antibody Cat.-No AM10220PU-N

Detection of SNARE Ti-VAMP protein (52 kDa) using Ti-VAMP antibody Cat.-No AM10220PU-N at 1/500 dilution in extract from Rat brain.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20151113 2 / 2