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2016_APW02_ZhangLimeng.pdf

Limeng Zhang1, Yanbi Yu2, Zhonglong Lin2, Zhihua Huang1

Yuxi Branch of Yunnan Company of CNTC, 653100, Oct. 11, 2016

1 Congress2016 - Document not peer-reviewed by CORESTA Tobacco production in Yunnan As one of the most important tobacco growing regions in the world, Yunnan has received worldwide reputation not only for its super-scale tobacco production but also excellent internal qualities of tobacco. 2016_APW02_ZhangLimeng.pdf

20% of the world 40% of China 40% of China

Yunnan Yunnan Yunnan

Tobacco leaf yield of the world Tobacco leaf yield of China Tobacco leaf export of China

2 Congress2016 - Document not peer-reviewed by CORESTA Tobacco production in Yunnan

/×104 ha /×104 Ton /×104 households 2016_APW02_ZhangLimeng.pdf

3 Congress2016 - Document not peer-reviewed by CORESTA The distribution of tobacco production in Yunnan

——Tobacco planting region in 2016_APW02_ZhangLimeng.pdf

云南烟叶质量安全控制情况介绍

4 Congress2016 - Document not peer-reviewed by CORESTA The distribution of tobacco production in Yunnan

——Tobacco planting region in 2016_APW02_ZhangLimeng.pdf

5 Congress2016 - Document not peer-reviewed by CORESTA The distribution of tobacco production inYunnan

——Tobacco planting region in Baoshan 2016_APW02_ZhangLimeng.pdf

6 Congress2016 - Document not peer-reviewed by CORESTA The distribution of tobacco production in Yunnan

——Tobacco planting region in Wenshan 2016_APW02_ZhangLimeng.pdf

云南烟叶质量安全控制情况介绍

7 Congress2016 - Document not peer-reviewed by CORESTA The distribution of tobacco production in Yunnan

——Tobacco planting region in Honghe 2016_APW02_ZhangLimeng.pdf

8 Congress2016 - Document not peer-reviewed by CORESTA The characteristics of Yunnan tobacco cultivation  Wide distribution of tobacco planting

 Stereoscopic climate and environment 2016_APW02_ZhangLimeng.pdf

 Smallholder farmers dominate the tobacco production landscape

 Different backgrounds for growers

 Many factors and constraints interact to tobacco leaf yields and quality

How to properly transmit the technology and applied science to the growers and field technicians in the tobacco production chain to produce tobacco leaf with the desired quality and yield able to meet the expectations of the tobacco and the growers? 9 Congress2016 - Document not peer-reviewed by CORESTA A case of Yunnan experience in dissemination of biocontrol technology to farmers and field technicians 2016_APW02_ZhangLimeng.pdf

Biological control for Myzus persicae with natural enemy --- Aphidius gifuensis

10 Congress2016 - Document not peer-reviewed by CORESTA The aphid is one of the main pests in agricultural production system including tobacco

Directly Indirectly 2016_APW02_ZhangLimeng.pdf

The aphid can not only reduce the quality of tobacco, but also vector viruses such as cucumber virus and many different potyviruses. 11 Congress2016 - Document not peer-reviewed by CORESTA Russia Canada 2016_APW02_ZhangLimeng.pdf Germany Italy North Korea U.S.A Spain South Korea India China The United Arab Burma ,China Emirates Philippines

The natural parasitic enemy——Aphidius gifuensis is widely distributed all over the world. 12 Congress2016 - Document not peer-reviewed by CORESTA The principle of Myzus persicae biological control technology with its natural enemy 2016_APW02_ZhangLimeng.pdf

Female A.G. Male A.G. Mating Searching for hosts Parasitizing

Hatching into A. gifuensis Eclosion Pupa Larvae parasitized mummy

The whole process lasts for 10 to 15 days. Aphids (Myzus persicae) died when the A. gifuensis hatched in the aphid body into a parasitized mummy. 13 Congress2016 - Document not peer-reviewed by CORESTA 2016_APW02_ZhangLimeng.pdf How to properly transmit this biocontrol technology to the growers and field technicians, and to equip smallholders with this technology and enable them to achieve greater performance?

14 Congress2016 - Document not peer-reviewed by CORESTA Step 1 Scientific research 1 Break through the winter breeding conservation problem of Aphid and Aphidius gifuensis.

 The proper host plant with highly resistance of TMV was selected such as Yunyan 2016_APW02_ZhangLimeng.pdf 203, radish, Chinese cabbage.

15 Congress2016 - Document not peer-reviewed by CORESTA Step 1 Scientific research

1 Broke through the winter breeding conservation problem of Aphid and

Aphidius gifuensis. 2016_APW02_ZhangLimeng.pdf

 Alternative winter breeding conservation methods were built.

Floating Seedling conservation Mature plant conservation Cool conservation system:good system:the characteristics of the system:a storage period, for long distance transportation, storage period is short, fast strong individual. cuting off the effect of Pachyneuron reproduction. aphidis (bouche), lower cost. 16 Congress2016 - Document not peer-reviewed by CORESTA Step 1 Scientific research

2 Solve the problem of degradation and Hyperparasitoids. 2016_APW02_ZhangLimeng.pdf  Using cycle selection method to do detoxification, purification and rejuvenation for aphid and Aphidius gifuensis.  Adopting the method of artificial strip, light trapping to eliminate hyperparasitism.  Improves the quality of Aphidius gifuensis individuals and groups.

Cycle selection Light trapping Artificial strip 17 Congress2016 - Document not peer-reviewed by CORESTA Step 1 Scientific research 3 Invent the technology of aphids inoculation and transfer: the main technical points were clear. 2016_APW02_ZhangLimeng.pdf Transfer time: Tobacco plants grow 6 - 8 effective leaves Innoculation method:picking, tearing, erasing, rod method. Innoculation quantity: 20-30 head/plant. Breeding conditions: Temperature: 17 ℃ to 27 ℃, humidity: 50%-80%. Breeding time:15 d-20 d.

Picking transfer Tearing transfer Erasing transfer Rod transfer method method method method 18 Congress2016 - Document not peer-reviewed by CORESTA Step 1 Scientific research

4 Establish the method of Aphidius gifuensis large-scale breeding .

70-80d 25-30d 15-20d 2016_APW02_ZhangLimeng.pdf

Seedling Transplanting Aphids rearing Parasitize 8-10d

7-10d

Releasing Collecting Aphidius gifuensis Hatching

Process of Aphidius gifuensis large-scale breeding by mature plant 19 Congress2016 - Document not peer-reviewed by CORESTA Step 1 Scientific research 5 Integrate the Aphidius gifuensis high-density breeding technology.

Rearing by 2016_APW02_ZhangLimeng.pdf rod transfer method mature larva

Aphids Parasitized Aphidius Releasing parasitism Rearing by mummy gifuensis erasing transfer method

Process of Aphidius gifuensis large-scale high density breeding by seedling plant 20 Congress2016 - Document not peer-reviewed by CORESTA Step 1 Scientific research

6 Develop efficient and convenient releasing methods. 2016_APW02_ZhangLimeng.pdf Collecting Storage Transportation Releasing

Products of parasitized Living A.fifuensis A.gifuensis auto-collector cards Cages aphid mummy transportation

Auto collecting & equipment Living A.gifuensis Seedling with parasitized Fixed and mobile releasing holder aphid mummy 21 Congress2016 - Document not peer-reviewed by CORESTA Step 2 Develop technical standardization 2016_APW02_ZhangLimeng.pdf

Extension based on the techical standardization 22 Congress2016 - Document not peer-reviewed by CORESTA Step 3 Training and Extension 2016_APW02_ZhangLimeng.pdf

Weifeng Zhang et al., 2016 Nature. Closing yield gaps in China by empowering smallholder farmers. 23 Congress2016 - Document not peer-reviewed by CORESTA Step 3 Training and Extension

Technology transmission system

Tobacco Production and Purchasing Scientific Research and Technical Extension System Management System 2016_APW02_ZhangLimeng.pdf Department of Science and Technology Provincial Tobacco Company

Academy of Tobacco Agricultural Sciences Municipal Tobacco Companies

Tobacco Production Technology Centers County branches Technical Extension Stations Tobacco Stations Technical Extension Sites 24 Congress2016 - Document not peer-reviewed by CORESTA Training system

Provincial 1 Technical

Companies Manager 2016_APW02_ZhangLimeng.pdf

Step-by-step Training Technical 我们Companies of States and Cities 2 Backbone

3 Field County Branches Technicians

4 Counselors Tobacco Station Leading farmers

25 Congress2016 - Document not peer-reviewed by CORESTA 1 The 1st level training for technical managers 2016_APW02_ZhangLimeng.pdf

Provincial level training: 100 technical managers were trained

26 Congress2016 - Document not peer-reviewed by CORESTA 2 The 2nd level training for technical backbones 2016_APW02_ZhangLimeng.pdf

Municipal level training: 1,000 technical backbones were trained

27 Congress2016 - Document not peer-reviewed by CORESTA 3 The 3rd level training for technicians 2016_APW02_ZhangLimeng.pdf

County level training: 2,000 technicians were trained

28 Congress2016 - Document not peer-reviewed by CORESTA 4 The 4th level training for counselor and leading farmers 2016_APW02_ZhangLimeng.pdf

Tobacco station level training: 460,000 growers were trained 29 Congress2016 - Document not peer-reviewed by CORESTA Extension organization 2016_APW02_ZhangLimeng.pdf

Yuxi Extension Station Dali Extension Station

Honghe Extension Station Wenshan Extension Station 30 Congress2016 - Document not peer-reviewed by CORESTA 2016_APW02_ZhangLimeng.pdf

Releasing parasitized seedling

Releasing living adults of Aphidius gifuensis 31 Congress2016 - Document not peer-reviewed by CORESTA 2016_APW02_ZhangLimeng.pdf

Parasitized aphid with seedling——releasing to field directly

32 Congress2016 - Document not peer-reviewed by CORESTA Parasitic natural enemy——Aphidius gifuensis Mass Rearing Station 2016_APW02_ZhangLimeng.pdf

33 Congress2016 - Document not peer-reviewed by CORESTA 2016_APW02_ZhangLimeng.pdf

Using the seedling float system to propagate the Aphidius gifuensis 34 Congress2016 - Document not peer-reviewed by CORESTA Stereo seedling system for Aphidius gifuensis mass rearing 2016_APW02_ZhangLimeng.pdf

35

35Congress2016 - Document not peer-reviewed by CORESTA Stereo seedling system for Aphidius gifuensis mass rearing 2016_APW02_ZhangLimeng.pdf

36

36Congress2016 - Document not peer-reviewed by CORESTA 6000-10000 Aphidius gifuensis per plants. 28 plants per small green house. 16.8 thousands Aphidius gifuensis per green house.

Releasing for 6 hectares. 2016_APW02_ZhangLimeng.pdf

37 Congress2016 - Document not peer-reviewed by CORESTA 2016_APW02_ZhangLimeng.pdf

38 Congress2016 - Document not peer-reviewed by CORESTA Extension in Yunnan and China

Extension area of Yunnan Extension area of Whole nation Extension ratio of Yunnan Extension ratio of Whole nation

2016_APW02_ZhangLimeng.pdf

100% 90 )

92.20% ) 90% 80

85.60% ha

80% 82.20% 4

% ( 70 10

70% 71.67% 60 × ( 60% 62.50% 54.10% 50 50% 40 40% 41.62% 30 30% 30.95% 31.45% Extension ratio 24.22% 23.61%

20% 20 Extension area 10% 12.07% 10 0% 0 2010 2011 2012 2013 2014 2015 39 Congress2016 - Document not peer-reviewed by CORESTA

Effects云南省各州市烟蚜茧蜂对烟蚜的防治效果(%) of aphids prevention with biological control

90

88 2016_APW02_ZhangLimeng.pdf

86

84

82

80

78

76

The average control effect (%) controlTheaverageeffect 昆明市玉溪市曲靖市红河州楚雄州大理州昭通市保山市文山州普洱市丽江市临沧市

40 Congress2016 - Document not peer-reviewed by CORESTA

2016_APW02_ZhangLimeng.pdf Aphids population density per plant plant Aphids population / Ln(n+1) density per Transplanting time 41 Congress2016 - Document not peer-reviewed by CORESTA

Pesticide 2016_APW02_ZhangLimeng.pdf

Releasing A. gifuensis

Control

control treatment / plant / treatment control

Field aphid populations with populations aphid Field

releasing A.gifuensis treatment /plant A.gifuensistreatment releasing Field aphid populations with pesticide andpesticide with populations aphid Field

Date The dynamic of field aphid populations 42 Congress2016 - Document not peer-reviewed by CORESTA Contrasting the cost of extension between pesticides and biocontrol

Treatments Costs Items 2016_APW02_ZhangLimeng.pdf

Costs of pesticides 90 ¥×3 times = 270 ¥ Pesticide 1,620 ¥ Costs of labor 450 ¥×3 times = 1,350 ¥

Costs of facilities 24.9 ¥

Biocontrol 87.9 ¥ Costs of mass rearing 33.0 ¥

Costs of releasing 30.0 ¥

43 Congress2016 - Document not peer-reviewed by CORESTA  Extending this biocontrol technology to other crop production: the effect of aphids control on average is nearly 60%. 2016_APW02_ZhangLimeng.pdf

Maize Rape Peas Sweat pea

44 Radish Cabbage Broccoli Pepper Peach

Broad bean 44 Congress2016 - Document not peer-reviewed by CORESTA 2016_APW02_ZhangLimeng.pdf

The tobacco industry and tobacco company are happy!

The farmers and growers are also happy! 45 Congress2016 - Document not peer-reviewed by CORESTA Research Science and Technology Training Extension 2016_APW02_ZhangLimeng.pdf Input Output

46 Congress2016 - Document not peer-reviewed by CORESTA Acknowledgements Tobacco production technology center, Yuxi tobacco company JI Sigui 2016_APW02_ZhangLimeng.pdf GU Xinghui ZhOU Wenbing Department of Science and Technology, Yunnan tobacco company YU Yanbi LIN Zhonglong CNTC ZHANG Hong WANG Deping Ming CORESTA Scientific Commission, JT International Germany GmbH

Marco Prat 47 Congress2016 - Document not peer-reviewed by CORESTA