Quality Assurance Project Plan (QAPP) for the Primary Headwater Habitat Study of Selected Ohio Streams

Division of Surface Water July 25, 2019 Quality Assurance Project Plan (QAPP) for the Primary Headwater Habitat Study of Selected Ohio Streams

July 25, 2019

Prepared by State of Ohio Environmental Protection Agency

Division of Surface Water Lazarus Government Center 50 West Town Street, Suite 700 P.O. Box 1049 Columbus, Ohio 43216-1049

Michael Dewine, Governor Lori Stevenson, Director

State of Ohio Ohio Environmental Protection Agency

A2 - Table of Contents

A2 – Table of Contents SECTION A – PROJECT MANAGEMENT ...... 3 A1 – Quality Assurance Project Plan ...... 3 A3 – Distribution List ...... 6 A3.1 – Table 1. Ohio EPA Central Office Staff ...... 6

A4 – Project/Task Organization and Communication ...... 7 A4.2 – Table 4. Roles & Responsibilities ...... 7

A4.2 – Figure 1. Organizational and Communication Chart ...... 8

A5 – Problem Definition/Background ...... 8 A6 – Project/Task Description ...... 9 A6.1 – Table List of Preliminary Sampling Locations ...... 9

A7 – Quality Objectives and Criteria ...... 9 A8 – Special Training/Certification ...... 10 A9 – Documents and Records ...... 10 A9.1 – Document/Record Control ...... 10

A9.2 – Document Storage ...... 11

SECTION B – DATA GENERATION AND ACQUISITION ...... 11 B1 – Sampling Process Design ...... 11 B2 – Sampling Methods ...... 11 B2.1 – Stream Habitat Evaluation ...... 11

B2.2 – Biological Community Assessment ...... 11

B2.3 – Field Parameters ...... 11

B2.4 – Thermal Regimes ...... 11

B3 – Sample Handling and Custody ...... 12 B4 – Analytical Methods ...... 13 B5 – Quality Control ...... 13 B6 – Instrument/Equipment Testing and Calibration, Inspection, and Maintenance ...... 13 B7 – Inspection/Acceptance of Supplies and Consumables ...... 14 B8 – Data Management ...... 14 B8.1 – Biological and Habitat Data Sheets ...... 14

B8.2 – Field Chemistry Samples ...... 14

B8.3 – Data Management Summary ...... 14

SECTION C – ASSESSMENT AND OVERSIGHT ...... 14 C1 – During Sampling Assessments/Analysis and Response Actions ...... 14 C1.1 – Assessments ...... 14

C1.2 – Response Actions ...... 15

C1.3 – Reporting and Resolution of Issues ...... 15

C1.4 – Data Completeness ...... 15

C2 – Reports to Management ...... 15 C2.1 – Headwater Habitat Evaluation and Biological Assessments ...... 15

SECTION D – DATA VALIDATION AND USABILITY ...... 16 D1 – Data Review, Verification, and Validation ...... 16 D2 – Verification and Validation Methods ...... 16 D3 – Reconciliation with User Requirements ...... 16 Appendices ...... 17 Appendix 1 – Table 1. Summary of Sampling Effort ...... 17 Appendix 2 – Temperature Probe Site List ...... 18 Appendix 3 – Sampling Locations ...... 19 Appendix 4 – Map of Public Lands ...... 37 Appendix 5 – Field Site Map ...... 38 Appendix 6 – Field Data Forms ...... 39-44 Appendix 7 – HOBO Temperature Logger Manual ...... 45-47 References ...... 48 A3 - Distribution List

A3.1 - Table 1. Ohio EPA Staff

Name/Title Contact E-mail/Phone

Chief or Assistant Chief [email protected] 614-644-2033

Marianne Mansfield, EAS Manager [email protected] 614-644-2876

Jeff Bohne, EAS Supervisor [email protected] 614-836-8798

Mike Bolton, Macroinvertebrate Biologist [email protected] 614-836-8781

Ed Moore, Macroinvertebrate Biologist [email protected] 614-836-8785

Greg Orr, DSW/NEDO [email protected] 330-963-1189

Kelly Capuzzi, DSW/SEDO [email protected] 740-380-5283

Jared Burson, DSW/CO [email protected] 614-721-8697

Ellie Hagen, Quality Assurance Coordinator [email protected] 614-705-1011

Audrey Rush, STS Manager [email protected] 614-644-2085

Chris Skalski, Water Quality Standards [email protected] 614-644-2144 A4 – Project/Task Organization and Communication A4.1 - Table 4. Roles & Responsibilities Individual(s) Assigned: Responsible for: Authorized to: Resolve disputes, suggest changes Allocate resources, project and edits, approve needed Chief or Asst. Chief implementation, resolve resources, approve overall project disputes. and QAPP Staff assignment, signatures, Marianne Mansfield, EAS payments, and reporting. Review documents and reports; Manager Confirm intermediate and final suggest changes and edits; obtain Jeff Bohne, EAS Supervisor milestones completed in a approvals and signatures. timely manner. QA/QC input to document Audrey Rush, WQS Manager development. Prepare Ellie Hagen documents and reports. Follow- Review documents and reports; Quality Assurance Coordinator up on deliverable delays and suggest changes and edits. their manager for ALU information.

STUDY TEAM Ensure QAPP revisions & distribution, oversee site Write and revise guidance. Develop Chris Skalski, Project Leader selection, coordinate field testing standards, coordinate field Water Quality Standards activities, field sampling and resources and assessments. analysis, and quality control.

Scheduling and coordination of Prepare documents and reports. Sarah Macy, field activities, field sampling Provide training to other team Mike Bolton, and analysis, quality control; members. Ed Moore, data collection, review, Schedule field activities, summarize Macroinvertebrate Biologists analysis, verification, database biological findings. population and transmission.

Scheduling and coordination of Jared Burson, Water Quality field activities, Participate in Review documents and reports. Standards site selection and assessment. Participate in site selection and Kelly Capuzzi, SEDO/DSW Review documents and reports. assessment.

Participate in site selection and Greg Orr, NEDO/DSW Review documents and reports. assessment. A4.2 - Figure 1: Organizational and Communication Chart

A5 – Problem Definition/Background The Federal Clean Water Act provides for "maintaining the biological integrity of the nation's waters" from the mouths to the headwaters. It is well established in the scientific literature that headwater streams are important to the quality of water and biological communities in larger streams to which these primary headwater streams are tributary. In carrying out the regulatory responsibilities for streams in the State of Ohio, a methodology has been developed to evaluate primary headwaters. The methodology is outlined in Ohio EPA’s Field Evaluation Manual for Ohio Primary Headwater Habitat Streams (2009), which is cited within the rules of Ohio’s Credible Data Program.

Ohio EPA needs to obtain baseline data from select primary headwater sites. Requirements of the existing Credible Data Program (CDP) have prompted the need for a list of primary headwater test sites to assess participant’s competency in primary headwater assessment techniques as outlined in Ohio EPA’s primary headwater manual. To test an initial applicant’s ability to properly perform the methodology, the applicant’s site assessment scores are compared to the findings of Ohio EPA experts. A concerted effort will be needed in 2018 and 2019 by Division of Surface Water to develop a list that provides adequate variety of site classes and settings (i.e. ecoregion) to properly assess the skills of applicants. The target is to develop a geographically varied list of approximately 125 sites.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 8 of 48 An adequate site list will accommodate the expanded testing requirements and lessen the foot traffic for these sensitive primary headwater habitats. A more robust site list distributed across the State will provide trainees with ample opportunity to have access to an assortment of testing locations while exposing them to the various quality headwater streams commonly encountered within each of the ecoregions in Ohio. In addition to the importance of providing a variety of stream types and settings, the site list is also intended to be large enough to provide that a high proportion of sites could be close to a potential applicant’s base of operations while still providing a network of sites representing conditions that may be different from other parts of the State. Finally, the testing network needs to be large enough such that different sites can be assigned to multiple individuals participating from the same organization to foster an independent competency assessment of each applicant.

The effort to build and maintain this test site list will also serve to build and maintain “bench strength” expertise in primary headwater assessment techniques amongst surface water staff. This expertise will serve to assist in future trainings of internal and external personnel, as well as grading of any tests that are part of the external application process. A core staff has been assembled, comprised of agency staff who were initially involved with the development of primary headwater methods and have ample experience utilizing the various primary headwater methods. The bench group is comprised of additional, selected surface water staff to ensure knowledge of headwater assessments is more integrated within the division of surface water and to better allow the long-term success of this methodology.

A6 – Project/Task Description The project team will assess a variety of headwater streams around the state using the methods described in the Ohio EPA Field Evaluation Manual for Ohio’s Primary Headwater Habitat Streams (2009), with the main objective to develop a network of potential testing sites to support the Division’s Credible Data Program (CDP). Development of this list will also allow less experienced staff to learn from the core group of experienced assessors to develop “bench strength” with these skills. A total of 143 sites have been initially identified for this project. Staff utilized GIS software to locate sites with drainages less than one square mile that were also situated on public lands. Most of the sites have never previously been field verified, so the current list may differ from the final test site list depending on what is encountered in the field.

Habitat quality (HHEI) and ambient biology will be collected at each of these sites. Measurements of water temperature, pH, conductivity and dissolved oxygen readings will be collected using a field meter. Continuous temperature measurements will be collected at sites where biological indicators suggest a strong influence of groundwater discharge to the stream. No other chemistry data is required to fulfill the objectives to this study. Scheduling of field work is done by the core group and must account for usual weather events and atypical stream flow conditions. Core group members should plan and schedule work considering the best time(s) when favorable stream conditions will likely be present. All aspects of coordination will be communicated with the project leader.

A6.1 - A list of preliminary sampling locations is provided in Appendix 1.

A7 – Quality Objectives and Criteria The data quality objectives of this study include:

1) Document the habitat features, biological assemblages (salamanders, fish, macroinvertebrates), chemical field parameters and thermal regimes of headwater streams across the state. The baseline information will be used to enable Ohio EPA’s Credible Data Program to properly determine if an applicant has the technical skills

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 9 of 48 necessary to assess and classify headwater streams. These assessments will be done in accordance with Ohio EPA’s Primary Headwater Manual.

2) Ongoing field training of additional surface water staff (bench group) on how to perform primary headwater assessments to develop bench strength amongst the division. This will ensure no method drift occurs over time and will enable the implementers of these protocols to mentor other staff when actively practicing.

3) Bolster the Agency’s current paucity of biological and habitat data from primary headwaters in the State of Ohio.

Assessors go out together in the field to collect the data. Each team is comprised of one or more core group member and may be accompanied by members of the bench group. The assessments are performed by more than one individual and results are discussed amongst the group to resolve any differences and to standardize scoring. The primary headwater field manual is always with a team member in the field and available for reference.

A8 – Special Training/Certification DSW has developed an Access database to document initial trainings and refreshers. All staff involved in collecting any type of environmental sample must complete training associated with that sampling method. The first line supervisors shall ensure staff have the necessary safety and skill set training (initial and refresher training) prior to sampling. All staff involved in this project have attended PHWH training and most have attended multiple training sessions which provided instruction in the various levels of PHWH assessments. Trainings for biological and habitat sampling is logged in the division’s TrainTrack Access database.

A9 – Documents and Records The final Quality Assurance Project Plan (QAPP) will be provided to the appropriate project personnel by email as detailed in the distribution list. As the plan is updated, each person on the distribution list will be sent an email with the most current document by the project leader. The most current date of revision will be included in the document name and in the header of the document.

The Primary Headwater Habitat Evaluation (HHEI) forms, PHWH Stream Biological Characteristics Field Sheets, Macroinvertebrate Scoring Sheets and temperature probe forms will be maintained in their original form (in paper files at DSW CO) and information from those forms, that are able to be uploaded, will be included in the Agency’s Ecological Assessment and Analysis Application (EA3) database. The EA3 database is backed up on a secure server. The forms which are unable to be uploaded into EA3 will be securely stored, in a project folder, on an agency hard drive and also uploaded to Ohio EPA’s Sharepoint site.

Field measurements taken with a YSI® Exo-1 multiprobe will be recorded electronically and will also be recorded on the HHEI field sheet. The data will be input manually into the database for storage and dissemination (any field sheets will be maintained in paper files at EAS). The data will be placed directly into Agency databases that have secure backup and ease of retrieval.

At the conclusion of the study, a summary of all the sampling results will be compiled and a finalized test site list will be developed for use in the credible data program.

A9.1 - Document/record control The recording media for the project will use a combination of waterproof paper and electronic means to document site conditions. Data gathered using paper will be recorded using indelible or waterproof ink, and changes to such data records

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 10 of 48 will be made by drawing a single line through the error with an initial by the responsible person. Similar methods will be used for electronic data recording.

The Project Leader shall retain the most recent version of the QAPP and be responsible for distribution of the current version of the QAPP to the project team. Agency management and the QA will approve updates to the QAPP, as needed. The Project Leader shall retain copies of all management reports, memoranda, and all correspondence between team members identified in Section A (these documents will be stored in the STS/Quality/QAPPs folder on the M drive).

A9.2 - Document storage The project leader will maintain a central project file, which will act as a repository for all data collected or generated as part of this project. The project file will include both electronic data (e.g., the QAPP and development or revision emails) and any hard copies and will be stored at the Ohio EPA office. Fish and Macroinvertebrate data will be the only data types stored in the Ecological Assessment and Analysis Application (EA3) database. All other data types (Salamander, Habitat, Field Parameters, and Temperature) will be maintained in paper and electronic formats at Central Office. All files will be retained by Ohio EPA indefinitely (for a minimum of 10 years).

SECTION B – DATA GENERATION AND ACQUISITION B1 – Sampling Process Design The Sampling Process for this project was designed to represent a wide variety of sites, based on classes (as described in the primary headwater guidance), ecoregions, and general geographic representation. The site list was developed by the project team, representing three offices of Ohio EPA/DSW. The initial list was compiled using GIS software that identified streams with drainages less than 1 sq. mile, located within areas with public access. It was then supplemented with known sites previously sampled by team members.

B2 – Sampling Methods All habitat, biological, chemical field parameters, data processing, and data analysis methods and procedures adhere to those specified in the Field Evaluation Manual for Ohio’s Primary Headwater Streams (Ohio EPA, 2009). Macroinvertebrate assessments are also consistent with the methods outlined in the Biological criteria for the protection of aquatic life: Volume III. Standardized biological field sampling and laboratory methods for assessing fish and macroinvertebrate communities (Ohio EPA, 2015).

B2.1 - Stream Habitat Evaluation Physical habitat will be evaluated using the Headwater Habitat Evaluation Index (HHEI) developed by the Ohio EPA for headwater streams in Ohio (Ohio EPA 2009). The HHEI is a rapid, visual assessment of instream physical habitat quality and is designed to predict biological potential in streams with drainage less than a square mile. The HHEI is comprised of three physical characteristics or metrics of the stream that contribute to a site habitat score. The three metrics include substrate type, average bankfull width, and maximum pool depth. These metrics were found to best correlate with habitat quality, and ultimately be predictive of biological potential, during the initial calibration and development of the HHEI technique. B2.2 - Biological Community Assessment Biological assessments will be conducted at each site assessed. These assessments examine fish, macroinvertebrates and salamander communities.

The Headwater Macroinvertebrate Field Evaluation Index (HMFEI) will be completed for each site following the methods outlined in Ohio EPA’s Primary Headwater Evaluation Manual (2009). In addition, a qualitative macroinvertebrate sample will be collected at each site following standardized methods outlined in Ohio’s biological criteria manuals (Ohio EPA, 2015). A voucher will be collected for each site following standardized methods used by Ohio EPA.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 11 of 48 Salamanders will be sampled at each stream location following the methods outlined in the Primary Headwater Habitat Manual (Ohio EPA, 2009). Sampling will include a search for all life stages to document the presence or absence of reproducing populations for each species encountered. A Visual Encounter Survey (VES) will be conducted to provide a semi-quantitative assessment of the salamander community present in each stream. In the event that any salamander(s) cannot be positively field identified, a voucher specimen will be retained for laboratory verification, as described in B.3 below. Salamanders may also be vouchered in order to supply testing kits, which will be used to during the initial certification process, to evaluate external data collector identification proficiency. Vouchering is subject to any restrictions that may be imposed by individual jurisdictions and in special situations may be limited to photographic vouchers.

Fish will be collected from streams as the hydrology allows using a backpack shocking unit. The presence of fish may be limited in some streams by a lack of suitable habitat or physical barriers to that impede fish passage. Fish will be identified to species level and counts of each species will be recorded on the PHWH biological characteristics field data sheet. In the event that any fish cannot be positively field identified, a voucher specimen will be retained for laboratory verification, as described in B.3 below.

B.2.3 Field Parameters Field water quality parameters will be recorded (but no chemical samples will be collected for lab analysis) by Ohio EPA personnel to obtain baseline water quality data at each of the sites. Data will be collected using a calibrated YSI Exo-1 field meter. The field meter will be used to collect the following data: ambient water temperature, dissolved oxygen, pH, and specific conductivity. Field meter calibrations are performed by experienced water quality staff before each day in the field. Resulting data will be recorded on the PHWH field data form.

B.2.4 Temperature sensors Temperature sensors will be deployed in 29 sites listed in Appendix 2. Sites selected for temperature sensor deployment are based on biological and habitat observations, with a goal of placing sensors in a set of streams supporting or likely supporting cold water fauna. The sensors will collect water temperature measurements at regular intervals throughout the duration of their deployment in the stream, generally lasting 2-3 months. Measurement intervals will be programmed to record the water temperature at a frequency of 30-minute intervals. Sensors will physically be placed in the stream as close to the location of the biological collections as possible, with practical and logistical considerations such as water depth and concealment opportunities also playing a role in exact placement based on field observations. Each sensor will be secured to the underside of the top of a cinder block using a zip tie or similar fastener. The block is in turn placed onto the bed of the channel flow or pool and secured with a wire cable pad-locked to a stable object. Sensors should be well concealed to minimize loss due to theft and vandalism. The stream temperature is recorded with a field meter or thermometer at time of deployment and retrieval. Notes, sketches, and photos are taken following deployment to facilitate relocation at the time of retrieval. Additional details describing the deployment and retrieval of the temperature data loggers and the analysis of resulting data is described in Section G of Appendix II of Ohio EPA’s Surface Water Field Manual Version 7.0 (2019).

B3 – Sample Handling and Custody YSI meters will measure field parameters, which will be stored directly on the device and will also be recorded on the field data sheets. Biological field forms will be stored with the HHEI field data sheets on the M:drive. Fish and Macroinvertebrate data will be uploaded into the EA3 database to serve as an electronic backup.

Macroinvertebrate vouchers will be collected by a macroinvertebrate biologist at each site. Macroinvertebrate specimens collected using qualitative sampling procedures outlined in the Biocriteria Volume III field manual are composited in a 4-ounce sampling jar, preserved with a 70% ethanol solution, and returned to the laboratory for subsequent analysis. Macroinvertebrate samples are stored indefinitely at the lab.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 12 of 48 Salamander vouchers will only be collected if necessary. Salamander specimens will be collected using the procedures outlined in the Primary Headwater Manual (Ohio EPA, 2009). Individuals are brought back to the lab alive and are humanely euthanized before being placed in a fixed solution.

Fish vouchers will only be taken in the event a positive identification can’t be made in the field. Fish specimens will be collected using procedures outlined in the Biocriteria Volume III Field Manual are fixed in a formalin solution. Fish are then rinsed in clean water, are placed in 35% alcohol solution for two to three weeks, then switched to a 50% alcohol solution for two to three weeks, and lastly placed in 70% aqueous solution of ethyl alcohol for permanent storage. Fish Samples are stored indefinitely at the lab.

B4 – Analytical Methods Field meters will be used to collect water quality data (pH, Conductivity, Dissolved Oxygen (mg/l and % saturation), and Temperature on the day(s) biological samples are collected. Measurements are recorded on the HHEI field data sheet. No other chemical water quality analyses will be performed as part of this study. Any organisms that are unable to be identified or processed in the field will be analyzed at the lab using the methods described in the previously cited Primary headwater manual. Sample labels are also described in that document.

B5 – Quality Control Core primary headwater staff that are part of the project team have attended, and conducted, multiple PHWH trainings. The core members were also those who were a part of the primary headwater method development and have many years of experience conducting headwater surveys. The members of the bench group have also attended a PHWH training and will work directly with at least one member of the core staff during this study. Additionally, a field QC day is scheduled at the beginning of the project to allow confirmation that various team members from different offices are scoring site parameters as similarly as possible and to clarify any specific questions. Quality control measures for fish, salamander, and macroinvertebrate sampling are described in the referenced primary headwater manuals (Ohio EPA, 2009).

The field manual will be brought in the field and consulted as needed. B6 – Instrument/Equipment Testing and Calibration, Inspection, and Maintenance The team leaders have several years of experience operating and maintaining most of the equipment to be used during this project. The team leaders will inspect the equipment prior to and during the sampling. The team leaders will ensure that all equipment remains in functional working condition.

Instrument/Equipment Testing and Calibration, Inspection, and Maintenance All instruments/equipment will be inspected and calibrated prior to use. All calibration solutions used will be checked for expiration dates before utilized. The appropriate calibration procedure, as specified in the instruments user manual, must be followed. All equipment is assigned a log book that will detail the equipment’s calibration and maintenance history. For more details see section D and Appendix II of the Surface Water Field Sampling Manual for Water Quality Parameters and Flow (Ohio EPA 2018). The YSI® EXO1 sondes will be calibrated in accordance with standard protocol prior to each day that the equipment is to be used. A standard provided by DES will be used to calibrate conductivity and pH. The oxygen sensor will be calibrated in ambient air. A log book is maintained for each sonde. This log book contains the date of each calibration and standardized pertinent information proving that the device is within specifications. Whenever daily calibration is not practical, the EXO1 sonde will be checked against calibration standards in the field and this will be noted in the calibration log book. If any of the sonde parameters do not conform to the specifications provided in the standard protocol, the sonde will be recalibrated, repaired or another unit will be used until the sonde is repaired or replaced. The calibration readings as well as the all repairs are entered into the log book.

Other equipment used will follow specifications provided in the biological and habitat methods cited.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 13 of 48 B7 – Inspection/Acceptance of Supplies and Consumables A list of supplies is located in attachment 2 of the Primary Headwater Manual (Ohio EPA, 2009), which has also been added to the QAPP appendix for reference.

Supplies and consumables will be inspected upon receipt by the field sampling teams. Nearly all the supplies utilized for this project are maintained and used during the normal business operations of the Ohio EPA. The field team leaders will be responsible for ensuring that all sample containers and all needed supplies and consumables are available in advance of all field work. It will be their responsibility to maintain and replenish stock. Consumable supplies include sample containers, ethanol and formalin preservatives, and miscellaneous field supplies such as distilled water, sampling gear disinfectant, disposable gloves, paper towels, and paper field forms. Field personnel will confirm that all reagents are within applicable shelf life. B8 – Data Management B8.1 – Biological and Habitat Data Sheets All original HHEI and Headwater Biological Sheets are filed at Ohio EPA Central Office, Division of Surface Water. Macroinvertebrate and Fish data from the headwater biological data sheets will be manually entered into the EA3 database system. Field data sheets are double-entered to reduce entry error rates. Each respective collector is responsible for final proofing, editing, and confirming correctness of all information before the data is approved in the EA3 database. Respective data collectors are also typically responsible for final approvals of data sheets in the EA3 database.

Salamander and HHEI data will not be able to be housed within the EA3 database. PDFs will be generated from the original field sheets and will be housed electronically on the M:drive, with other relevant documents.

B8.2 – Field Chemistry Samples Field measurements are collected instantaneously using a handheld meter following the methods described in the Field Manual. The handheld meters have an internal file storage system that allows for data to be saved in the field by selecting the correct station from a site list created within the instrument’s menu system. Alternatively, the parameters can be recorded manually on the paper HHEI field form.

The temperature logger data will be uploaded electronically following retrieval of the devices. The data will be housed in an excel file and stored with all the other project data.

B8.3 – Data Management Summary The project leader maintains the project file in a dedicated folder on the M:drive>STS>PHWH_Project_2018 . The goal or objective is to have a complete record of all decisions about modifications of data collection, validation or interpretation between the QAPP signoff and project report completion. To achieve this, the project leader is included on emails or otherwise receives summaries of all actions that meet the above description. Project photos should all be filed in the Lynx photo management system.

SECTION C: ASSESSMENT AND OVERSIGHT C1 – During Sampling Assessments/Analysis and Response Actions C1.1 – Assessments Periodic assessment of field sites and field equipment is necessary to ensure that sampling goes according to plan, and data obtained meets project needs. This is an ongoing process that continues every day on which the project is implemented as well as larger scale assessments that take place less frequently (e.g., annually). The assessments generally will focus on readiness and consistency of implementation but also are looking for continual improvement opportunities.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 14 of 48 Daily assessments (for each day of project activities, as applicable) will include assessment of field equipment and supplies, completeness of the day’s samples and associated field notes, future needs, etc. C1.2 - Response Actions Despite best preparations staff performing assessments may find situations requiring corrective actions (CAs). Small day- to-day level assessment findings are often addressed by the individual doing the assessment in the field or in the lab and are common enough to the process, so as not to necessitate a formal response.

Corrective action implementation will be determined by the likelihood that the situation may affect the quality of the data. Field corrective actions will be brought to the attention of the study team for consideration as to their impact on the data.

C1.3 - Reporting and Resolution of Issues Any audits or other assessments that reveal findings of practice or procedure that do not conform to the written QAPP will be corrected as soon as possible. The Study Team and QA Coordinator will be notified regarding deviations. C1.4 - Data Completeness Success of the project will require collection of all listed data at all, or nearly all, of the listed locations. If all sampling locations are unable to be evaluated, it is imperative that an ample representative number of sites were assessed within each ecoregion to meet the stated objectives of this project. Potential data gaps will be monitored as the project progresses and the project schedule will be revised to fill these gaps where they are determined to be significant or to potentially impact the fulfillment of project objectives. The assigned biological crew leaders should contact Chris Skalski if weather or other circumstances arise that place completion of the planned work in jeopardy. Changes that substantially alter the sampling detailed in the QAPP should be emailed to the project team and potentially result in a QAPP revision (that will be distributed to those on the list).

C2 – Reports to Management Monthly (or as needed) oral progress reports are to be provided to management on the survey/study and what steps are being taken to resolve any issues or problems. This may include access problems early on that lead to changes of sites and weather or resource problems during sampling, which could lead to project delay. After the samples have been evaluated, the team leader and project ecologists will have a meeting to evaluate, compile and review findings. A final, comprehensive headwater test site list will be developed and circulated to individuals seeking certification.

C2.1 – Headwater Habitat Evaluation and Biological Assessments Complete and accurate headwater habitat assessment data along with a site’s biological findings are essential to achieving the data quality objectives identified in A7. It is the assessment data that enable Ohio EPA to have the baseline site information needed to properly test external collectors seeking certification in headwater assessments.

The various attributes of the available habitat are scored based on their overall importance to the establishment of aquatic faunas. Evaluations of the type and quality of the substrate, pool depth and bankfull width are the metrics used to evaluate the characteristics of a headwater stream segment.

Three types of biological assessments will be conducted at each of the primary headwater site locations. These include evaluations of the macroinvertebrate, fish and salamander communities. Biological indicators and community composition will provide additional information to pair with the habitat evaluation.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 15 of 48 SECTION D: DATA VALIDATION AND USABILITY D1 – Data Review, Verification, and Validation All habitat, biological, field parameter, and continuous temperature data will be filed in a centralized location and saved electronically. The data which can be entered into EA3 (Fish and Macroinvertebrate) will be hand-entered into the database using a double data entry method. This helps ensure issues due to data entry errors are minimized. Final approval of data involves a reconciliation between the paper forms and the electronic data which is completed by the data collector or a database administrator in the Ecological Assessment Section (EAS).

Upon approval in EA3, field data cannot be revised without intervention from database administrators in the Agency’s Office of Information Technology Services.

D2 – Verification and Validation Methods Biological and habitat field sampling results will be verified and validated based on field staff experience and qualifications, and adherence to training and QA/QC procedures for current and new field staff available in Subsection 1, Part A (macroinvertebrates) and Subsection 2, Part A (Fish and Habitat) in Biological Criteria for the Protection of Aquatic Life: Volume III. Standardized Biological Field Sampling and Laboratory Methods for Assessing Fish and Macroinvertebrate Communities (June 2015, available at: http://epa.ohio.gov/dsw/bioassess/BioCriteriaProtAqLife.aspx)). The study team will make final decisions regarding validity and usability and will evaluate the sample collection, analysis, and data reporting processes to determine if the data is of sufficient quality to meet the project objectives. Data validation involves all procedures used to accept or reject data after collection and prior to use. These include screening, editing, verifying, and reviewing. Data validation procedures ensure that objectives for data precision and bias will be met, that data will be generated in accordance with the QAPP and SOPs, and that data are traceable and defensible. The process is both qualitative and quantitative and is used to evaluate the project as a whole.

D3 – Reconciliation with User Requirements Issues related to biological and habitat data uncertainty, including any patterns of analytical or field QC uncertainties, will be assessed by field staff and their management. For most situations, issues can be addressed with acknowledgement of factors captured in the sample metadata which can confirm, explain, and document the data quality concern. Significant, persistent, or unresolved issues will be brought to the attention of the project Study Team, division QC personnel, and EAS and/or DSW management for further evaluation. This combination of personnel will assess how to best label affected data for storage in the agency hard drive and the EA3 database and how to eliminate or limit any similar problems going forward. Consideration will also be given on how best to memorialize data limitations or anomalies as the data is transferred to other databases, including STORET, so that future users of the sampling data are aware of any data quality issues or limitations.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 16 of 48 APPENDICES

Appendix 1 – Table 1. Summary of Sampling Effort Type of Sample # Sites # Passes Total # Physical Habitat (HHEI) (H) 143 1 143 Macroinvertebrate (HMFEI) (Mq) 143 1 143 Salamander (VES) (S) 143 1 143 Fish (Headwater) (F1) 143 1 143 Field Chemical Parameter Measurements 143 1 143 (FM) Continuous Temperature Monitoring (T) 29 1 29

F-single pass fish sampling VES - Salamander H – physical habitat assessment T – Temperature probe monitoring Mq- macroinvertebrate qualitative sampling FM -Field Measurements

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Ohio EPA, Division of Surface Water page 17 of 48 Appendix 2 – Temperature probe deployment locations

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 18 of 48

Appendix 3 – Sampling Locations Location Description HUC -12 Drainage Latitude Longitude Ecoregion Park Area

Trib. to Mosquito Creek (RM 50800010702 0.24 40.191835 -83.980243 ECBP Kiser Lake State 8.5)adjacent Kiser Lake SW Parking Park Area

Trib. to Mosquito Creek (RM 50800010702 0.36 40.190049 -83.977508 ECBP Kiser Lake State 8.65)near Kiser Lake SW Parking Park Area

Trib. to Mosquito Creek (RM 50800010702 0.1 40.193437 -83.974214 ECBP Kiser Lake State 8.7)near Kiser Lake NW Parking Park Area

Trib. to Mosquito Creek (RM 50800010702 0.7 40.18191 -83.949468 ECBP Kiser Lake State 10.10)east end of Kiser Lake, N of Park campground

Trib. to E. Fk. L. Miami R. (RM 50902021203 0.98 39.011455 -84.145036 IP East Fork State Park 21.18)4th tributary west of main beach parking area via hiking trail

Trib. to E. Fk. L. Miami R. (RM 50902021203 0.66 39.032967 -84.128498 IP East Fork State Park 22.90)1st tributary east of Elk Lick Road boat ramp parking

Trib. to Poplar Creek (0.53)2nd trib 50902021201 0.68 38.996156 -84.096105 IP East Fork State Park west of SE boat ramp parking area off of Reisinger Road

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 19 of 48 Trib. to Queer Creek (RM 4.42)ust. 50600020902 0.45 39.426224 -82.573775 WAP Hocking State SR 664, Hamilton Hollow Forest

Slate Lick (Trib. to Alum Cr. (RM 50600011403 0.5 40.311982 -82.944257 ECBP Alum Creek State 37.84))ust. Bald Lick and Trib. (RM Park 0.38); access via parking lot across Hogback Road

Bald Lick (Trib. to Alum Cr. ( RM 50600011403 0.53 40.312812 -82.944057 ECBP Alum Creek State 37.84, 0.30))Trib. to Slate Lick (RM Park 0.30); access via parking lot across Hogback Road

Trib. to Big Run (RM 0.24)north of 50600011403 0.75 40.270637 -82.982766 ECBP Alum Creek State parking area on Lackey-Old State Park Road at Curve Road

Trib. to Big Run (RM 2.26)south of 50600011403 0.16 40.252296 -82.985353 ECBP Alum Creek State parking area on Lackey-Old State Park Road at Berlin Station Road

Trib. to Alum Creek (RM 30.03)dstr 50600011404 0.16 40.238472 -82.982832 ECBP Alum Creek State parking area at campground Park welcome center at Cheshire Rd and Old State Rd

Trib. to Alum Creek (RM 50600011404 0.59 40.201963 -82.980638 ECBP Alum Creek State 27.77)northwest of the picnic area Park on the southwest side of the lake off park road accessed from Lewis Center Road

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 20 of 48 Trib. to Alum Creek (RM 27.77, 50600011404 0.42 40.198725 -82.978938 ECBP Alum Creek State 0.64)southwest of the picnic area Park on the southwest side of the lake off park road accessed from Lewis Center Road

Trib. to L. E. Fk Ohio Brush Creek 50902010501 0.76 38.976267 -83.4774 IP Tranquility Wildlife (RM 1.76)dst. from Trib (RM 1.76, Area 0.09) entering from south side.

Trib. to L. E. Fk Ohio Brush Creek 50902010501 38.97396 -83.47791 IP Tranquility Wildlife (RM 1.76, 0.09)trib to trib from Area south side (CR 100)

Trib. to Queer Creek (RM 6.13, 50600020902 0.51 39.432728 -82.547097 WAP Hocking Hills State 0.59)at SR 664, Hadden Hollow Park

Trib. to Queer Creek (RM 6.13, 50600020902 0.19 39.436286 -82.54277 WAP Hocking Hills State 0.94)west of parking and picnic Park area, ust. SR 664

Trib. to Queer Creek (RM 6.13, 50600020902 0.33 39.438984 -82.537808 WAP Hocking Hills State 1.14)directly north of Old Man's Park Cave campground, ust. SR 664

Trib. to Rattlesnake Creek (RM 50600030407 0.11 39.270804 -83.411234 ECBP Paint Creek State 0.87)west of boat loading zone off Park Snake Rd

Trib. to Rattlesnake Creek (RM 50600030407 0.45 39.273517 -83.419699 ECBP Paint Creek State 1.38)at the fork in Snake Road Park

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 21 of 48 Trib. to Paint Creek (RM 42.93)east 50600030603 0.12 39.267402 -83.385967 ECBP Paint Creek State of parking lot off Taylor Rd Park

Trib. to Cowan Creek (RM 5.48)at 50902020605 0.88 39.385159 -83.881077 ECBP Cowan Lake State Dalton Road Park

Trib. to Cowan Creek (RM 5.33)dst. 50902020605 0.24 39.387195 -83.886253 ECBP Cowan Lake State from park entrance road off Park Osborn Road

Trib. to Cowan Creek (RM 50902020605 0.13 39.379103 -83.889507 ECBP Cowan Lake State 5.22)accessed from Emerald Park Woods Trail

Trib. to Cowan Creek (RM 4.88)ust. 50902020605 0.13 39.380037 -83.896325 ECBP Cowan Lake State Yankee Road and ust. trib. Park

Trib. to Four Mile Creek (RM 50800020604 0.18 39.571387 -84.738197 ECBP Hueston Woods 22.31, 0.48)ust from Acton Lake State Park

Trib. to Four Mile Creek (RM 50800020604 0.84 39.571029 -84.737058 ECBP Hueston Woods 22.31)dst. from Trib. RM 0.60 State Park

Trib. to Four Mile Creek (RM 50800020604 0.56 39.572141 -84.736146 ECBP Hueston Woods 22.31)ust. from Trib. RM 0.60 State Park

Trib. to Four Mile Creek (RM 50800020604 0.63 39.57328 -84.75607 ECBP Hueston Woods 22.9)ust. from trail in Hueston State Park Woods State Nature Preserve

Trib. to Ninemile Cr. (RM 1.21, 50902011208 0.13 39.04351 -84.318756 IP Woodland Mound 1.15)east of Nordyke Rd

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 22 of 48 Trib. to Ninemile Cr. (RM 1.21)east 50902011208 0.18 39.044616 -84.318892 IP Woodland Mound of Nordyke Rd, across from park entrance drive

Trib. to Ninemile Cr. (RM 50902011208 0.37 39.043299 -84.320424 IP Woodland Mound 1.21)west of Nordyke Rd

Trib. to West Fork (RM 2.54, 50902030105 0.23 39.1585 -84.5829 IP Mt. Airy Forest 0.72)ust. from mouth

Trib. to West Fork (RM 2.54, 50902030105 0.19 39.15667 -84.59138 IP Mt. Airy Forest 1.04)dst. from Westwood Northern Blvd.

Trib. to West Fork (RM 3.05)west 50902030105 0.16 39.170301 -84.574319 IP Mt. Airy Forest of Trail Ridge Rd

Shepherd Creek (West Fk 50902030105 0.72 39.173673 -84.579649 IP Mt. Airy Forest 3.38)NEAR MT. AIRY, ADJ SHEPHERD RD

Trib. to Shepherd Creek (RM 50902030105 0.13 39.176731 -84.575857 IP Mt. Airy Forest 0.35)north of Blue Spruce Road

Trib. to Dry Fk Whitewater River 50800030809 0.11 39.251926 -84.764506 IP Miami Whitewater (RM 5.60)S of Timberlake Dr. Forest

Trib. to Dry Fk Whitewater River 50800030809 0.31 39.252276 -84.759162 IP Miami Whitewater (RM 6.02)south of West Rd Forest

Trib. to Dry Fk Whitewater River 50800030809 0.57 39.25561 -84.751 IP Miami Whitewater (RM 6.54)north of intersection of Forest

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 23 of 48 Habor Ridge Dr and Timberlakes Dr, west of campgrounds

Trib. to Shenango R. (RM 50301020104 0.2 41.54928 -80.528057 EOLP Pymatuning State 67.61)dst. Pymatuning Lake Rd Park

Trib. to Shenango R. (RM 68.30)at 50301020104 0.21 41.560535 -80.530363 EOLP Pymatuning State Pymatuning Lake Rd Park

Trib. to E. Br. Cuyahoga R. (RM 41100020101 0.65 41.520011 -81.103477 EOLP Headwaters Park 92.15)SE of East Claridon, within Headwaters Park

Trib. to E. Br. Cuyahoga R. (RM 41100020101 0.48 41.525898 -81.105494 EOLP Headwaters Park 92.8)SE of East Claridon, within Headwaters Park

Trib. to E. Br. Cuyahoga R. (RM 41100020101 0.17 41.531919 -81.106242 EOLP Headwaters Park 93.13)E of East Claridon, S of Mayfield Rd/SR 322

Pierson CreekW of Holden 41100030401 0.72 41.60847 -81.308521 EOLP Holden Arboretum Arboretum visitor center

Trib. to Pierson Creek (RM 41100030401 0.16 41.607516 -81.305223 EOLP Holden Arboretum 1.59)Dst. from Blueberry Pond

Trib. to Pierson Creek (RM 41100030401 0.2 41.61526 -81.31262 EOLP Holden Arboretum 0.77)West of Pierson Creek (MOVED ORIGINAL SITE [Trib to Pierson RM 0.74] TO THIS SITE. ALL INFORMATION IS NOW UPDATED)

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 24 of 48 Trib. to W. Br. Mahoning R. (RM 50301030304 0.17 41.14935 -81.131803 EOLP West Branch State 13.33, 0.7, 1.05)NE of bridle trail Park parking

Trib. to W. Br. Mahoning R. (RM 50301030304 0.13 41.151298 -81.130112 EOLP West Branch State 13.33, 0.7)NE of bridle trail parking Park

Trib. to W. Br. Mahoning R. (RM 50301030304 0.36 41.147788 -81.128708 EOLP West Branch State 13.33, 0.7)Unnamed trib to West Park Branch Mahoning River/ West Brach Reservoir RM 13.33 east of Horsemans Day Parking, fork to mouth

Trib. to W. Br. Mahoning R. (RM 50301030304 0.15 41.152359 -81.125608 EOLP West Branch State 13.33, 0.95, 0.54)Unnamed trib at Park RM .95 of unnamed trib at RM .64 of unnamed trib to West Branch Mahoning River/West Branch Reservoir RM 13.33

Trib. to N. Br. Kokosing River (RM 50400030101 0.18 40.50432 -82.58444 EOLP Kokosing Lake 9.85)upst. Waterford Rd Wildlife Area

Trib. to N. Br. Kokosing River (RM 50400030101 EOLP Kokosing Lake 9.85)at Waterfork Road Wildlife Area

Trib. to N. Br. Kokosing River (RM 50400030101 0.19 40.50432 -82.59306 EOLP Kokosing Lake 10.0/0.8)west of Waterford Rd Wildlife Area

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 25 of 48 Trib. to N. Br. Kokosing River (RM 50400030101 0.39 40.51569 -82.58985 EOLP Kokosing Lake 10.8)dst. from Waterford Rd Wildlife Area

Trib. to Odell Cr. (RM 1.85)east of 50902010202 0.15 38.727704 -83.110359 WAP Shawnee State Shawnee Trail Forest

Trib. to Odell Cr. (RM 1.97)Long 50902010202 0.34 38.727035 -83.119664 WAP Shawnee State Hollow, west of Shawnee Trail Forest

Trib. to Odell Cr. (RM 2.26)Walker 50902010202 0.27 38.732115 -83.113659 WAP Shawnee State Hollow, east of Shawnee Trail Forest

Trib. to Odell Cr. (RM 2.55)Zorns 50902010202 0.67 38.736804 -83.116615 WAP Shawnee State Hollow, east of Shawnee Trail Forest

Stony CreekDownstream Stewart 50600021005 0.75 39.21968 -82.9588 WAP Scioto Trail State Lake, south of picnic area off Park Stoney Creek Rd

Stony CreekUpstream Stewart 50600021005 0.11 39.215855 -82.966683 WAP Scioto Trail State Lake and tributary, southwest of Park picnic area off Stoney Creed Rd

Trib. to Stony Creek (4.08)South 50600021005 0.16 39.219576 -82.963491 WAP Scioto Trail State fork of unnamed trib to unnamed Park trib to Stewart Lake from NE, NW of Stoney Creek Rd picnic area

Trib. to Stony Creek (4.08, 50600021005 0.16 39.220782 -82.962871 WAP Scioto Trail State 0.1)North fork of unnnamed trib to Park unnamed trib to Stewart Lake

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 26 of 48 from NE, NW of Stony Creek Rd picnic area

Trib. to Big Run (5.84)Unnamed 50400060602 0.43 40.061836 -82.026049 WAP Powelson Wildlife trib to Big Run RM 5.9 adjacent to Area Northpointe Dr, north of interection of Northpointe Dr and Dresden Rd

Trib. to Big Run (5.84, 50400040305 0.22 40.067776 -82.02299 WAP Powelson Wildlife 3.08)Upstream Northpoint Road Area

Trib. to Town Fork (8.64)Unnamed 50301010801 0.12 40.467444 -80.798395 WAP Jefferson Lake State trib to Jefferson Lake/Rippy Run Park RM 9.55, east of 261A and just west of State Park Mooretown Rd

Trib. to Town Fork (8.75)Unnamed 50301010801 0.27 40.469016 -80.80494 WAP Jefferson Lake State trib to Jefferson Lake/Rippy Run Park RM 9.65, east of 261A

Trib. to Town Fork (9.84)Town 50301010801 0.82 40.468089 -80.814974 WAP Jefferson Lake State Fork west of 261A Park

Trib. to Spruce Run (RM 50600020604 0.4 39.469034 -82.597668 WAP Hocking State 2.06)south of parking area, Ryan Forest Hollow

Salt Well Run ust. of Hogback Rd 50600011403 0.57 40.322084 -82.949517 ECBP Alum Creek State Park

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 27 of 48 Trib. to West Fk Mill Cr. (RM 50902030102 0.38 39.253583 -84.506325 ECBP Winton Woods 7.19)east of parking lot off of horse trail

Trib. to Blair Run (0.47)Tributary to 50301061208 0.29 39.815912 -80.832786 WAP Sunfish Creek State Ohio River, at Krebs Ridge Rd Forest

Trib. to Pike Run (7.15, 50600020904 0.4 39.386532 -82.758765 WAP Tar Hollow State 1.35)Unnamed tributary to Tar Park Hollow Lake, upst gravel road near seating area

Trib. to Pike Run (7.15, 1.35, 50600020904 0.16 39.386585 -82.760091 WAP Tar Hollow State 0.58)Unnamed tributary to Tar Park Hollow Lake, upst western bend gravel road

Trib. to Pike Run (7.15, 50600020904 0.24 39.394064 -82.757841 WAP Tar Hollow State 1.93)Unnamed tributary, west of Park cul de sac of camping area

Trib. to Pike Run (7.15)Unnamed 50600020904 0.32 39.397951 -82.75386 WAP Tar Hollow State tributary, north of cul de sac of Park camping area

Trib. to Rocky Fork (3.90)Unnamed 50400050404 0.16 40.171449 -81.499853 WAP Salt Fork State Park tributary to Salt Fork Lake, upst of Twp Rd 855

Trib. to Salt Fork (6.05, 50400050406 0.1 40.105704 -81.503251 WAP Salt Fork State Park 0.75)Unnamed trib (southern fork)

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 28 of 48 to Salt Fork Lake, NW of parking area (40.104338, -81.500436)

Trib. to Salt Fork (6.05)Unnamed 50400050406 0.15 40.10652 -81.504879 WAP Salt Fork State Park trib (northern fork) to Salt Fork Lake, NW of parking area (40.104338, -81.500436)

Trib. to Salt Fork (12.88)Unnamed 50400050406 0.13 40.115341 -81.481374 WAP Salt Fork State Park tributary to Salt Fork Lake, dst of R-52

Trib. to Salt Fork (6.12)Unnamed 50400050406 0.28 40.094071 -81.53849 WAP Salt Fork State Park tributary, north of Twp Hwy 638, below fork of ephemeral channel

Trib. to Harris Ditch 41000090802 0.56 41.461415 -83.884533 HELP Maumee State (4.52)Unnamed trib to Harris Ditch Forest 4.52 west of Henry Lucas County Rd

Trib. to Harris Ditch (5.24, 41000090802 0.15 41.469595 -83.890652 HELP Maumee State 0.40)Right fork of south fork of Forest unnamed trib to Harris Ditch RM 5.20, east of Township Rd 1d

Trib. to Harris Ditch (5.24)East fork 41000090802 0.58 41.472349 -83.903015 HELP Maumee State of unnamed trib to Harris Ditch Forest RM 5.20, west of County Rd 2

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 29 of 48 Trib. to Harris Ditch (5.24, 41000090802 0.18 41.472107 -83.904545 HELP Maumee State 0.91)Unnamed trib to east fork of Forest unnamed trib to Harris Ditch RM 5.2, west of County Rd 2

Trib. to Swan Creek (RM 29.5)Dst. 41000090703 0.66 41.55956 -83.864976 HELP Oak Openings Monclova Rd., east of parking area Metropark on Monclova Rd., east of SR 64

Trib. to Swan Creek (RM 29.69, 41000090703 0.5 41.557455 -83.873686 HELP Oak Openings 0.37)The site looks ponded, there Metropark may be a stream farther upstream, maybe follow Springbrook Lake Trail or come S from Monclova Rd. to trail.

Trib. to Swan Creek (RM 29.69, 41000090703 0.17 41.558199 -83.874028 HELP Oak Openings 0.42)Stream is due W from parking Metropark lot, trails cross it NW and SW from parking lot.

Trib. to Swan Creek (RM 29.3)Dst. 41000090703 0.1 41.556711 -83.86494 HELP Oak Openings from Wabash-Cannonball Trail, E Metropark of Waterville-Swanton Rd., park in Oak Openings Springbrook Parking Lot.

Trib. to Ninemile Cr. (RM 8.86)Adj. 041000100501 0.26 41.387951 -83.279902 HELP Ringneck Ridge WA Swartzman Rd.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 30 of 48 Trib. to Ninemile Cr. (RM 041000100501 0.2 41.3888768 -83.279398 HELP Ringneck Ridge WA 8.86/0.22)near mouth, at Swartzman Rd.

PHWH TRIB. TO SLATE RUN 50600011805 0.3 39.7569056 -82.8420111 ECBP Columbus Metro (3.67/0.30)AT SLATE RUN Parks METROPARK

TRIBUTARY TO MARIE DELARME 41000050203 0.1 41.233855 -84.667884 HELP Forrest Woods CREEK (RM 1.4)dst. From C-73 Nature Preserve

SPRING CREEKSE OF BOSTON, ADJ. 41100020405 0.6 41.2556 -81.5786 EOLP Akron Metro Park BOSTON MILLS RD

DEERLICK RUNIN BEDFORD 41100020504 0.9 41.3761 -81.5456 EOLP Bedford Metropark METROPARK @ PARK DRIVE

WOODIE BROOKJUST DST. 41100030304 0.1 41.56189 -81.219288 EOLP Woodiebrook WOODIEBROOK RD. Preserve

TRIB. TO CHAGRIN R. (23.93)NEAR 41100030402 0.7 41.4553 -81.4066 EOLP S. Chagrin SP MOUTH

PHWH TRIB. TO CLEAR FK. 50400020405 0.8 40.6108306 -82.3182861 EOLP Mohican State Park MOHICAN R. (RM 4.00)NEAR MOUTH

HOG RUNUPST. ST. RT. 26 50302010705 0.8 39.485903 -81.270437 WAP Wayne NF

Trib. To WOLF RUN (2.76)UPST. 50302010901 0.7 39.8053 -81.5158 WAP Wolf Run SP WOLF RUN LAKE, UPST CO. RD. 14

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 31 of 48

TRIB. TO S. FK. SUGAR CREEK 50400011005 0.7 40.6181 -81.6222 WAP Beach City WA (4.07)N OF DUNDEE, dst. Camp Road (TWP. RD 446)

TRIB. TO S. FK. SUGAR CREEK (RM 50400011005 0.93 40.61224 -81.62484 WAP Beach City WA 4.07/0.65)Dst. from Camp Road

TRIB. TO CARBONDALE CREEK 50901010301 0.2 39.3836 -82.2753 WAP Zaleski SF (0.67)N OF CARBONDALE @ MOUTH

WAGGONER RUNSW OF TULIP, 50902010506 0.3 38.71966 -83.43224 IP Edge of Appalachia UPST. ABNER HOLLOW RD.

Mackenzie Runupst. Waggoner 50902010506 0.6 38.7195 -83.448044 IP Edge of Appalachia Riffle Rd.

Trib to W. Br. St. Joseph River (RM 41000030204 0.42 41.69392221 -84.70235231 ECBP Lake La Su Ann WA , RM 11.0)CR S

Trib to Prairie Ditch (RM 2.27)W 41000010303 0.86 41.661226 -83.789197 HELP Secor Metropark Tupelo Way

Trib to Blue Creek (RM 8.81)upst 41000090801 0.63 41.51627892 -83.89748183 HELP Maumee SF RL tributary

Trib. to Grand River (RM 20.83)at 41100040605 0.18 41.74672413 -81.07372306 EOLP Lake Metroparks River Rd Lake Metroparks

Trib. to Paine Creek (RM 5.62)off 41100040604 0.67 41.68710879 -81.1277481 EOLP Lake Metroparks Kniffen Rd/CR 208

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 32 of 48

Trib. to Turkey Creek (RM 1.2)from 41201010409 0.4 41.96019729 -80.52178316 EOLP Ashtabula County State Line Rd Metroparks

Trib. to E. Br. Rocky R. (RM 41100010201 0.12 41.217245 -81.717548 EOLP Hinkley Reservation 23.68)Hinkley Reservation

Trib. to Forked Run (RM 50302020404 0.24 39.11953477 -81.80659639 WAP Shade River SF 5.24)adjacent to Shade River SF Rd 9/T-265

Trib. to Watson Creek (RM 50901010706 0.5 38.60375238 -82.37307688 WAP Crown City WA 1.0)Crown City Wildlife Area

Trib. to L. Darby Creek (RM 50600012006 0.6 39.90412175 -83.24828236 ECBP Columbus Metro 2.09)from path off Gerich Lilly Rd Parks

Trib. to Nettle Creek (RM 7.67)ust 50800011603 0.31 40.1371694 -83.90522458 ECBP Davey SNP Nettlecreek Rd in Davey SNP

Trib. to Flat Branch (RM 5.21)dst. 41000080301 0.83 40.8197997 -83.62799165 ECBP ODNR Wildlife from CR 2

Trib. to Old Woman Creek (RM 41000120304 0.47 41.34183135 -82.49316813 EOLP Edison Woods 2.01)ust. Ceylon Rd Metropark

Riding Runust. Wheatley Road ust. 41100020403 0.6 41.21358556 -81.59795286 EOLP Riding Run trib. (0.6) Metropark

Trib. to Big Creek (RM 16.85)in Mel 41100040606 0.2 41.57713555 -81.185911 EOLP Mel Harder Park Harder Park

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 33 of 48 Trib. to L. Cuyahoga River (RM 41100020303 0.2 41.07742174 -81.44631164 EOLP Goodyear Heights 7.97)Parcours Trail in Goodyear Metropark Heights Metro Park

Trib. to L. Beaver Creek (RM 50301010609 1 40.73156847 -80.61653227 WAP Beaver Creek SP 14.68)adj. Echo Dell Road

Trib. to Brush Creek (RM 5.57)ust. 50301010804 0.67 40.55729 -80.79351 WAP Brush Creek WA T61

Puntenney RunAdj. Puntenney Run 50902010209 1 38.707354 -83.3888992 WAP Edge of Appalachia Rd., downstream Thompson Hollow

Trib. to Marie DeLarme Creek (RM 41000050203 0.01 41.233426 -84.66465 HELP Forrest Woods 1.35)East of C-73 Nature Preserve

Trib. to Grass Run (RM 0.36)South 41000060303 0.53 41.556584 -84.35302 ECBP Goll Woods SNP of County Rd. F, east of parking lot off County Rd. 26

Trib. to Tiffin River (RM 44.10)East 41000060303 0.26 41.554436 -84.364932 ECBP Goll Woods SNP of County Rd. EF, south of cottages on ODNR Rd.

Trib. to Stillwater River (RM 50800011405 0.02 39.88101 -84.285565 ECBP Englewood 9.51)South of Patty Rd. shelter, on Metropark Red Trail

Trib. to Trib. to Stillwater River 50800011405 0.19 39.885049 -84.282936 ECBP Englewood (RM 9.51)North of Patty Falls Rd., Metropark at Patty Falls

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Water Version 1.0, July 25 2019 page 34 of 48 Trib. to Stillwater River (RM 50800011406 0.86 39.869771 -84.275806 ECBP Englewood 8.62)South of Aullwood Audubon Metropark Center and Farm on Aullwood Rd.

Trib. to Sugar Creek (RM 3.33)At 50902020501 0.25 39.61964 -84.100949 ECBP Sugar Creek Sycamore Ride Park Feature, north Metropark of Conference Rd.

Trib. to Twin Creek (RM 50800020306 0.13 39.639022 -84.403388 ECBP Germantown 10.11)North of Germantown Metropark Dam/Conservancy Rd., at Trail Intersection 16

Trib. to Twin Creek (RM 50800020306 0.5 39.641791 -84.42534 ECBP Germantown 11.34)South of the Germantown Metropark Metropark Nature Center, off Boomershine Rd.

Trib. to Twin Creek (RM 50800020306 0.56 39.637565 -84.418215 ECBP Germantown 10.85)Southeast of the Metropark Germantown Metropark Nature Center, off Boomershine Rd., east of Trail Intersection 9

Trib. to Twin Creek (RM 50800020306 0.12 39.636749 -84.416109 ECBP Germantown 10.70)Southeast of the Metropark Germantown Metropark Nature Center, off Boomershine Rd., along Orange/Gray Trail

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Version 1.0, July 25 2019 page 35 of 48 Trib. to Twin Creek (RM 50800020306 0.1 39.577186 -84.353235 ECBP Twin Creek 2.35)Woodland Naturary west of Metropark Morningstar Rd. and Little Turtle Campsite

Trib. to Silver Creek (RM 41100030301 0.37 41.4587 -81.3033 EOLP West Woods 3.50)South of Kinsman Rd., west of West Woods Nature Center and ASM International Park

Trib. to Silver Creek (RM 3.23) 41100030301 0.87 41.4621 -81.3114 EOLP West Woods South of Kinsman Rd., directly south of Wiegands Lake.

Puntenney Run Adj. Puntenney 50902010209 0.5 38.70803 -83.38898 WAP Edge of Appalachia Run Rd., upstream Thompson Hollow

Trib. to Silver Creek (RM 41100030301 0.61 41.4568 -81.3258 EOLP West Woods 2.23)Stream by Affelder House

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Ohio EPA, Division of Surface Version 1.0, July 25 2019 page 36 of 48 Appendix 4 – Map of Public Lands

Map displaying all public areas within the state of Ohio and their proximity to Ohio EPA’s five district offices. PHW sites evaluated as part of this project are being conducted on public lands.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 37 of 48 Appendix 5 – Field Site Map

Statewide view of the project’s site distributions and how they are represented within each of Ohio’s five major ecoregions

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 38 of 48 Appendix 6 – Field Data Forms

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 39 of 48 PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 40 of 48 PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 41 of 48 PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 42 of 48 PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 43 of 48 PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 44 of 48 Appendix 7 – HOBO Temperature Logger Manual

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 45 of 48 PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 46 of 48 PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 47 of 48 REFERENCES Hughes, R.M., D.P. Larsen, and J.M. Omernik. 1986. Regional reference sites: a method for assessing stream pollution. Env. Mgmt. 10(5): 629-635

Karr, J.R. and D.R. Dudley. 1981. Ecological perspective on water quality goals. Env. Mgmt. 5(1): 55-68. Ohio Environmental Protection Agency. 2019. Surface water field sampling manual for water quality parameters and flows. Div. of Surface Water, Columbus, Ohio. Version 7.0, April 22, 2019. . 2009. Field evaluation manual for Ohio's primary headwater habitat streams. Version 2.3. Ohio EPA Division of Surface Water, Columbus, Ohio. 86 pp.

. 2015b. 2015 Updates to Biological criteria for the protection of aquatic life: Volume II and Volume II Addendum. User’s manual for biological field assessment of Ohio surface waters. Div. of Surface Water, Ecol. Assess. Sect., Columbus, Ohio. May 8, 2015.

. 2015c. Biological criteria for the protection of aquatic life: Volume III. Standardized biological field sampling and laboratory methods for assessing fish and macroinvertebrate communities. Tech. Rept. EAS/2015-06-01. Div. Of Surface Water, Ecol. Assess. Sect., Columbus, Ohio. September 23, 2015. . 1989a. Addendum to Biological criteria for the protection of aquatic life: Volume II. User’s manual for biological field assessment of Ohio surface waters. Division of Water Quality Planning and Assessment, Surface Water Section, Columbus, Ohio. . 1989b. The qualitative habitat evaluation index (QHEI): rationale, methods, and application. Division of Water Quality Planning and Assessment, Columbus, Ohio. . 1987. Biological criteria for the protection of aquatic life: Volume II. User’s manual for biological field assessment of Ohio surface waters. Division of Water Quality Monitoring and Assessment, Surface Water Section, Columbus, Ohio.

Omernik, J.M. 1988. Ecoregions of the coterminous United States. Ann. Assoc. Amer. Geogr. 77(1): 118-125.

PHWH Sampling - Selected Ohio Streams 2018-2019 QAPP Version 1.0, July 25 2019 Ohio EPA, Division of Surface Water page 48 of 48