Final Report: Lower Peninsula Healthy Creeks Project Grassroots Ecology (formerly Acterra Stewardship)

Funded by the 2014 SAFE, CLEAN WATER PRIORITY B GRANT PROGRAM Santa Clara Valley Water District Agreement Number: A3758R Grassroots Ecology is a fiscally sponsored project of Acterra: Acon for a Healthy Planet

Formerly known as the Acterra Stewardship Program, we became Grassroots Ecology on July 1, 2016. For over 30 years, we have served the community with high quality volunteer‐based environmental stewardship programs. We are passionate about our mission of involving, educang, and inspiring the public to create healthy ecosystems in our urban communies, natural lands, and waters.

Our team engages 10,000 people annually in hands‐on restoraon, cizen sci‐ ence, and educaonal programs in the Silicon Valley region. We are grassroots by name and grassroots by philosophy. It is by working and learning together that we, as a community, have the power to reinvigorate our local part of the planet

Grassroots Ecology 3921 East Bayshore Road Palo Alto, CA 94303 [email protected] 650‐419‐9880 Lower Peninsula Healthy Creeks Project

Thank You!

Grassroots Ecology (formerly known as Acterra Stewardship) was honored to receive a $68,600 grant from the Santa Clara Valley Water District’s 2014 Safe, Clean Water Priority B Grant Program. From August 2014 through September 2016 monies from this grant funded a community‐based collaborave effort to provide hands‐on wa‐ tershed educaon and stewardship acvies to reduce trash, pescides, and other toxic chemicals in our creeks.

Deliverables Completed:  24 volunteer water quality monitoring events on Stevens, San Francisquito (and its tributaries), Matade‐ ro, Barron, and Adobe Creeks. 17 events on Permanente Creek.  2 volunteer fall benthic macroinvertebrate sampling events at five sites on Stevens Creek.  47 volunteer “Bug Club” events to process the Stevens Creek benthic macroinvertebrates.  10 student benthic macroinvertebrate sampling events on Permanente Creek, plus extensive lab work (Bug Week) at Mountain View High School  6 student benthic macroinvertebrate sampling events on Stevens Creek, plus extensive lab work (Bug Week) at Monta Vista and Alta Vista High Schools.  27 hands‐on field trips  10 World Water Monitoring Challenge events  8 quarterly Watershed Forums  10 newsleers  Project celebraon (presentaon to District Board to be completed at their convenience)  10 volunteer creek cleanups

Measurable Outcomes:  4,225 parcipants (1,305 volunteers and 2,920 educaon parcipants)  High quality data for 23 water monitoring sites and 7 benthic macroinvertebrate sites  14.75 miles of riparian areas cleared of trash.  18,180 pounds of trash collected

The following pages describe our programs and the wonderful support we had from the community to improve the health of our local creeks.

Alexandra Von Feldt Execuve Director, Grassroots Ecology Task 1: Volunteer Water Quality Monitoring

The health of all the creeks in our heavily urbanized nia Environmental Data Exchange Network where valley is impaired due to pescides and other toxic it is available to those making decisions on creek chemicals. Most homeowners are unaware of the management. part they play in contribung to this toxicity. It may It is not all work for the volunteers at our events. seem that each of us does not contribute much to Our local creek areas are quite lovely and full of water polluon,e but th cumulave impact of the home use of pescides and ferlizers, washing the car in the driveway, not picking up pet waste, and allowing cars to leak oil and radiator fluids is huge. Grassroots Ecology’s volunteer water quality moni‐ toring program addresses the problem at several levels. At the outset of each event, we talk to the parcipants about just where water polluon comes from. Most people have no idea that rain‐ water flowing off their property carries toxic chem‐ icals. And they have not made a connecon be‐ life—wildflowers, birds, bugs, and other wildlife tween the water that flows into the local storm provide plenty of entertainment and enjoyment. We think the experience encourages the volun‐ teers to place a greater value on creek habitats and a desire to protect them. Mother Nature threw us some surprises so we needed to adjust our monitoring sites a bit throughout the course of the grant period. When we discovered New Zealand Mud Snails in Stevens Creek, we pulled the Permanente Creek site from the monthly event in which we also monitor Ste‐ vens Creek so we would not cross‐contaminate. drain and the water in the creeks. We help them We substuted in another Permanente Creek site connect the dots as far as how what they do at associated with one our riparian restoraon pro‐ home affects creek health. jects and monitored it on different days. We then train the volunteers to collect data on wa‐ Also, the drought caused a significant dry back on ter chemistry—temperature, pH, dissolved oxygen, Stevens Creek for an extended period of me. To specific conductance, and turbidity. In all, our vol‐ compensate, we added in some extra sites at the unteers collected data from 25 sites on six creeks— lower end of the creek where the water table is Stevens, Permanente, San Francisquito, Barron, high enough that the channel is always wet. Please Adobe, and Matadero. We follow careful calibra‐ refer to the appendix at the end of this report to on and field protocols so that our data is robust. view the water chemistry data we collected for the We are working to upload the data to the Califor‐ six creeks during the grant period. Task 2: Volunteer Aquac Habitat Monitoring

Most people do not give much thought to the vari‐ depth, benthic parcle size, weed channel width, ous lile insects, worms, clams, and other crea‐ canopy cover, flow, and water chemistry. tures which live at the boom of their local creek. The year the drought dried up much of Stevens Our volunteers know that these creatures— Creek—both above and below the dam—we sub‐ formally known as benthic macroinvertebrates, but stuted in sites at the very lower end of the creek usually just called bugs—are used by biologists where the channel is always wet. Since this area around the world to monitoring creek health. Since has dal influence, it was interesng to see the some bugs are polluon sensive, while others are difference in bugs. This area was thick with lile quite tolerant, the types of bugs we find at a creek crustaceans; we decided that if we were steelhead site can provide us with important informaon. trout, this is where we would like to hang out. Twice a month throughout the year, we meet with our volunteers at what we like to call “Bug Club” to process our benthic samples. Using a dichotomous key we put together featuring the local bugs (see appendix) and a picture guide, our volunteers iden‐ fy the bugs to family level, counng the number of each.

We began our annual sampling of Stevens Creek bugs in 2006 in a formal partnership with the US Geological Survey. That partnership has expired, but we have connued the study under the guid‐ ance of Steven Fend, a stream ecologist and ento‐ mologist. Using the targeted riffle method, we col‐ lect five one square foot samples of benthos from each site. We also collect physical habitat data—

Since our “Bug Years” do not exactly coincide with the grant period years, we have not yet finished processing all the bugs we collected in the fall 2015 sampling. Therefore, the data included in the ap‐ pendix at the end of the report shows the results of the 2013 and 2014 sampling events. Task 3: Watershed Educaon for Students

Four years ago we adapted the benthic macroin‐ Before the students started work on the bug study vertebrate, or creek bug, study we had been doing we visited each class to talk about how watersheds with community members for high school students work and how each of us impacts the health of lo‐ to evaluate the health of their own local creeks. cal creeks. To demonstrate how pollutants from The overarching goal of the program was not just our yards reach the creek, students took part a hands‐on exercise which resulted in a sasfyingly big mess. The students discussed how they could help the health of the creek by making changes in their daily acvies and how the bug study they will be conducng will provide informaon on how well we are protecng aquac habitat health. Each pair of students was then provided with a tray of squirming live bugs we had brought from the

to teach students about creek science, but to pro‐ vide a means for them to do creek science.

In the first year of the contract period with SCVWD we delivered the program to Biology students at Monta Vista and Mountain View High Schools. Be‐ cause of teacher requests, we extended the pro‐ gram to include Alta Vista and Cindy Avia High Schools in the second year. In all, we reached creek, a magnifying lens, and a cheat sheet so they could begin to learn who’s who in bug world. A lot of the students had never turned over a creek rock before to look at the wriggly things on the boom and so had no idea that the creek was so full of life. In addion to using these acvies as a way to in‐ troduce Biology students to the full bug study, we brought the same acvies as a stand‐alone experi‐ ence to Earth Science, Life Science, and AP Environ‐ mental Studies classes. Students were then invited to join us at a later date to conduct the bug sampling. The field work is al‐ 1,530 students with the program—1,035 with the ways a lot of fun, but the students do follow care‐ full bug study and 495 with an introducon to ful protocols similar those we use in our communi‐ creek ecology. ty volunteer study. The students also collect a vari‐ Task 3: Watershed Educaon for Students

The following days we move on to sorng and idenfying the bugs. Each team of two students has their own sample which they process during the course of the week. On the final day we ana‐ lyze the results. This study is quite challenging for high school students, but they are fully up to it. We can see their confidence grow throughout the week as they discover they can do real science. Because we are working with hundreds of students over a relavely short period of me, we cannot maintain quite the same quality control as we can with our community volunteer study where the data is quite robust. Nevertheless, the data we get in the student study prey much represents the expected results for these sites. For instance, the data from Permanente Creek show significantly beer aquac habitat at the “Kids Crossing” site on the West Fork of Permanente which is not below the Lehigh Cement Plant. As part of our student outreach, we also run hands‐ on water quality and streamside bug analysis field ety of physical habitat data—water chemistry, trips for college students at sites along Stevens depth, benthic parcle size, weed channel width Creek. and canopy cover. Processing the bugs and analyzing Spring 2016 Permanente Creek Bug Study the results is a prey big deal so 60% this takes a whole week of lab me. To the uniniated, all bugs 50% look rather alike so thet firs day we do prey intensive training on 40% bug idenficaon. We have de‐ % Sensitive Bugs (more is veloped a PowerPoint introduc‐ 30% better) on which is enlivened with fun % Sensitive Families (more facts—did you know an adult 20% is better) male mayfly is the only insect on Earth to have two, um, male re‐ 10% % Dominant Taxa (less is better) producve bits?—and is support‐ ed with Bug Bingo (see appendix). 0% Kids Horse Bay Tree Lupine Heritage Crossing Bridge Fork Oaks Task 4: Community Outreach and Educaon

Our World Water Monitoring Challenge events are color‐changing kits to measure the dissolved oxy‐ a favorite with community members. These events gen, pH, temperature, and turbidity of water in the are aimed at bringing young families to local creeks creek. Just like field biologists, they enter their to learn how watersheds work, how important findings on a data sheet. creeks are, and how we can all help to make them We have a special bug staon so the parcipants healthier. also get to conduct a streamside assessment of We start off each event by having the parcipants aquac habitat quality using benthic macroinverte‐ invesgate residenal sources of polluon with our brates. The very young children just like playing Enviroscape—an interacve watershed model. with the creek bugs, but school‐age children through adults use the “cheat sheets” we provide to idenfy the bugs as polluon‐tolerant or pollu‐ on‐sensive.

When the “rain” comes via spray boles, the pollu‐ tants run downslope making an icky mess of the water body at the lower end. It is prey dramac to see li le plasc fish swimming in the muck. We The spring of 2015 provided us with an extra chal‐ all talk about ways we can reduce that polluon. lenge in that one of our usual event sites on Ste‐ We then have the parcipants visit five staons to vens Creek was quite dry. We brought in buckets of learn about how healthy—or polluted—the real creek water and bugs from higher in the watershed creek is. The kids and their parents use hands‐on, where the channel was sll weed and held the event right in the dry channel. This turned out to be just as much fun as holding a regular event and gave us all a chance to talk about water conserva‐ on as well.

Also, as part of our community outreach we put out newsleers highlighng watershed events and educaon, held quarterly Watershed Forums for community members to discuss watershed issues, and put on special events such as the “Fish Day” we had at McClellan Ranch. Task 5: Community Creek Cleanups

Each fall and spring, Grassroots Ecology led volunteers into areas along Stevens, Permanente and Adobe Creeks to clean out the accumulated trash. Some of the trash was from local sources—homeless people, hikers and picnickers—but much looked as if it had washed down from upstream areas or entered the creeks through the storm drains. Part of the educaonal value of these events was our discussion with the volunteers as to how trash moves around. It was surprising to the younger parcipants to think about how a drink straw they toss down at their school could enter the storm drain, wash in the creek, and then be carried out to the ocean. Much of what we found was food containers. It was sasfying to see, though, that plasc bags were way down over previous years. Empty liquor boles were plenful. We got a bit too much informaonb from a cu scout upon seeing a pile of empty vodka boles. His eyes widened and he exclaimed, “Vodka, my mom loves vodka!”

Construcon materials were not uncommon; it is sad to see that some people are obviously using the creek as a convenient dump site when building or remodeling. Some things we found—a cross bow and a sequined dress for instance—led our volunteers to come up with interesng stories as to how they got there. Appendix of Materials Related to: Lower Peninsula Healthy Creek Project

Funded by the 2014 Safe, Clean Water Priority B Grant Program

 Water Chemistry Data—Stevens, Permanente, San Francisquito, Barron, Adobe, and Matadero Creeks

 Stevens Creek Benthic Macroinvertebrate Data

 Curriculum Samples for Watershed Educaon for Students

 Data Sheet & Supervisor Instrucon Sheets for World Water Monitoring Challenge Events Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 14-Sep-14 Moss Rock 15.54 1.440 7.16 7.73 1.50 14-Sep-14 Moss Rock 15.53 1.439 7.45 7.65 1.00 14-Sep-14 Dam Outlet 22.18 0.794 0.14 7.74 32.10 14-Sep-14 Dam Outlet 22.17 0.792 0.14 7.67 33.80 14-Sep-14 Chestnut 18.73 0.786 6.73 7.85 25.60 14-Sep-14 Chestnut 18.74 0.787 6.58 7.81 26.70 14-Sep-14 McClellan 17.05 0.884 7.98 7.91 4.50 14-Sep-14 McClellan 17.05 0.879 8.11 7.89 4.90 14-Sep-14 101 21.56 0.926 8.45 7.84 2.20 14-Sep-14 101 21.60 0.927 8.76 7.81 2.10 14-Sep-14 La Avenida 19.88 0.972 7.51 7.68 1.50 14-Sep-14 La Avenida 19.91 0.971 7.40 7.65 1.70 14-Sep-14 Trailer Pk Outfall 21.03 1.002 6.72 7.70 5.50 14-Sep-14 Trailer Pk Outfall 21.12 0.997 6.64 7.70 5.30

5-Oct-14 Moss Rock 13.32 0.902 8.03 7.59 0.40 5-Oct-14 Moss Rock 13.32 0.907 8.25 7.57 1.70 5-Oct-14 Dam Outlet 20.22 0.598 5.23 7.82 11.40 5-Oct-14 Dam Outlet 20.23 0.596 5.54 7.79 10.90 5-Oct-14 Chestnut 16.68 0.596 8.14 7.72 56.90 5-Oct-14 Chestnut 16.69 0.596 8.37 7.68 58.50 5-Oct-14 McClellan 15.30 0.608 8.22 7.91 4.10 5-Oct-14 McClellan 15.30 0.607 8.22 7.89 3.80 5-Oct-14 101 23.04 0.723 17.04 7.83 2.30 5-Oct-14 101 23.10 0.724 17.15 7.87 2.10 5-Oct-14 La Avenida 19.03 0.766 8.83 7.51 1.40 5-Oct-14 La Avenida 19.05 0.766 8.82 7.51 1.80 5-Oct-14 Trailer Pk Outfall 20.37 0.770 8.16 7.75 2.90 5-Oct-14 Trailer Pk Outfall 20.45 0.769 8.28 7.68 3.10

9-Nov-14 Moss Rock 10.41 1.387 9.52 7.90 0.70 9-Nov-14 Moss Rock 10.41 1.387 9.46 7.85 0.60 9-Nov-14 Dam Outlet 15.25 1.229 3.96 7.72 65.10 9-Nov-14 Dam Outlet 15.24 1.228 3.96 7.71 67.00 9-Nov-14 Chestnut 13.52 0.078 8.59 7.73 46.60 9-Nov-14 Chestnut 13.53 0.104 8.60 7.87 47.10 9-Nov-14 McClellan 12.43 1.238 8.67 8.00 7.60 9-Nov-14 McClellan 12.44 1.238 8.63 8.01 7.00 9-Nov-14 101 21.07 1.305 13.19 8.01 3.30 9-Nov-14 101 21.09 1.379 12.86 8.00 3.80 9-Nov-14 La Avenida 18.58 1.486 9.04 7.85 3.10 9-Nov-14 La Avenida 18.63 1.487 8.56 7.85 2.90 9-Nov-14 Trailer Pk Outfall 19.14 1.501 8.28 7.92 1.80 9-Nov-14 Trailer Pk Outfall 19.19 1.501 8.69 7.89 2.00

7-Dec-14 Moss Rock 11.89 0.470 11.00 8.04 4.70 7-Dec-14 Moss Rock 11.89 0.471 10.96 8.09 4.60 7-Dec-14 Dam Outlet 13.36 0.615 5.08 7.60 51.10 Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 7-Dec-14 Dam Outlet 13.36 0.613 5.21 7.59 51.70 7-Dec-14 Chestnut 13.42 0.624 8.06 7.71 20.40 7-Dec-14 Chestnut 13.40 0.625 7.88 7.70 20.80 7-Dec-14 McClellan 13.46 0.689 8.72 7.85 5.80 7-Dec-14 McClellan 13.47 0.689 8.72 7.87 4.00 7-Dec-14 101 17.95 0.750 8.89 7.72 2.40 7-Dec-14 101 19.94 0.741 8.88 7.71 2.20 7-Dec-14 La Avenida 17.51 0.796 7.15 7.68 1.70 7-Dec-14 La Avenida 17.53 0.798 7.22 7.68 1.90 7-Dec-14 Trailer Pk Outfall 18.27 0.934 4.78 7.52 2.90 7-Dec-14 Trailer Pk Outfall 18.29 0.934 4.78 7.52 2.20

11-Jan-15 Moss Rock 8.02 0.560 12.25 8.16 0.90 11-Jan-15 Moss Rock 8.03 0.556 12.23 8.17 0.70 11-Jan-15 Dam Outlet 9.67 0.463 5.55 7.55 61.50 11-Jan-15 Dam Outlet 9.68 0.461 5.54 7.55 61.90 11-Jan-15 Chestnut 9.89 0.493 10.12 7.74 48.60 11-Jan-15 Chestnut 9.90 0.494 10.23 7.76 48.90 11-Jan-15 McClellan 9.32 0.602 10.54 7.89 14.30 11-Jan-15 McClellan 9.39 0.605 10.65 7.88 13.50 11-Jan-15 101 15.56 0.762 18.96 8.20 1.20 11-Jan-15 101 15.72 0.764 18.85 8.24 1.60 11-Jan-15 La Avenida 16.02 0.805 11.58 8.01 1.40 11-Jan-15 La Avenida 16.07 0.805 10.87 7.95 1.50 11-Jan-15 Trailer Pk Outfall 15.53 0.822 11.15 7.99 2.90 11-Jan-15 Trailer Pk Outfall 15.56 0.819 11.27 8.00 2.50

15-Feb-15 Moss Rock 9.35 0.394 12.39 8.05 3.00 15-Feb-15 Moss Rock 9.37 0.395 12.38 8.05 3.40 15-Feb-15 Dam Outlet 10.18 0.379 3.78 7.23 23.70 15-Feb-15 Dam Outlet 10.18 0.377 3.77 7.20 24.10 15-Feb-15 Chestnut 10.84 0.384 10.31 7.56 22.70 15-Feb-15 Chestnut 10.87 0.383 10.32 7.57 22.50 15-Feb-15 McClellan 10.07 0.415 11.59 7.94 27.10 15-Feb-15 McClellan 10.08 0.414 11.51 7.89 29.60 15-Feb-15 Stevens Ck. Blvd. 12.04 0.430 11.65 7.93 23.10 15-Feb-15 Stevens Ck. Blvd. 12.08 0.430 11.58 7.84 23.20 15-Feb-15 West Valley Elem. 11.83 0.464 11.41 8.05 7.70 15-Feb-15 West Valley Elem. 11.85 0.464 11.35 8.04 8.10 15-Feb-15 La Avenida 18.53 0.733 9.63 7.69 1.10 15-Feb-15 La Avenida 18.62 0.733 9.23 7.70 1.00

15-Mar-15 Moss Rock 11.60 0.600 11.39 8.22 0.80 15-Mar-15 Moss Rock 11.62 0.600 11.35 8.20 0.80 15-Mar-15 Dam Outlet 11.33 0.503 7.20 7.51 16.40 15-Mar-15 Dam Outlet 11.33 0.503 7.15 7.43 16.40 15-Mar-15 Chestnut 11.87 0.503 10.37 7.75 20.30 15-Mar-15 Chestnut 11.88 0.503 10.37 7.78 19.40 Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 15-Mar-15 McClellan 12.11 0.541 10.32 7.80 14.90 15-Mar-15 McClellan 12.11 0.541 10.46 7.84 14.00 15-Mar-15 Stevens Ck. Blvd. 13.84 0.544 10.93 8.07 11.10 15-Mar-15 Stevens Ck. Blvd. 13.82 0.546 11.00 8.03 10.30 15-Mar-15 La Avenida 18.95 0.966 10.18 7.79 2.50 15-Mar-15 La Avenida 19.01 0.965 10.67 7.80 1.80

12-Apr-15 Moss Rock 9.49 0.487 12.51 8.19 0.90 12-Apr-15 Moss Rock 9.50 0.485 12.34 8.18 0.80 12-Apr-15 Dam Outlet 12.03 0.390 8.19 7.39 15.20 12-Apr-15 Dam Outlet 12.03 0.392 8.12 7.38 15.10 12-Apr-15 Chestnut 12.15 0.390 10.45 7.78 14.80 12-Apr-15 Chestnut 12.17 0.392 10.58 7.76 14.20 12-Apr-15 McClellan 11.12 0.447 11.22 7.89 16.70 12-Apr-15 McClellan 11.14 0.447 11.17 7.91 16.30 12-Apr-15 Stevens Ck. Blvd. 12.89 0.426 11.92 8.09 8.90 12-Apr-15 Stevens Ck. Blvd. 12.86 0.426 11.67 8.07 9.90 12-Apr-15 West Valley Elem. 12-Apr-15 West Valley Elem. 12-Apr-15 Evelyn 12-Apr-15 Evelyn 12-Apr-15 La Avenida 18.16 0.751 9.51 7.71 0.90 12-Apr-15 La Avenida 18.23 0.750 9.50 7.75 1.30

17-May-15 Moss Rock 11.67 0.585 11.15 8.13 0.80 17-May-15 Moss Rock 11.71 0.587 11.09 8.04 1.10 17-May-15 Dam Outlet 12.71 0.469 7.63 7.18 13.80 17-May-15 Dam Outlet 12.71 0.467 7.62 7.34 13.60 17-May-15 Chestnut 13.00 0.466 10.24 7.78 13.40 17-May-15 Chestnut 12.97 0.467 10.37 7.76 12.80 17-May-15 McClellan 12.21 0.499 10.36 7.06 14.90 17-May-15 McClellan 12.21 0.498 10.34 7.15 15.00 17-May-15 Stevens Ck. Blvd. 13.36 0.489 12.68 8.13 9.00 17-May-15 Stevens Ck. Blvd. 13.35 0.488 12.82 8.22 8.30 17-May-15 La Avenida 18.15 0.892 7.53 7.77 1.70 17-May-15 La Avenida 18.25 0.889 7.53 7.73 1.90

7-Jun-15 Moss Rock 14.99 0.607 9.73 7.94 0.80 7-Jun-15 Moss Rock 15.03 0.606 9.56 7.96 0.70 7-Jun-15 Dam Outlet 13.16 0.497 8.24 6.66 13.70 7-Jun-15 Dam Outlet 13.16 0.497 8.11 6.65 13.60 7-Jun-15 Chestnut 13.67 0.478 9.77 7.63 16.60 7-Jun-15 Chestnut 13.68 0.478 9.86 7.59 16.80 7-Jun-15 McClellan 14.28 0.485 9.83 7.98 13.70 7-Jun-15 McClellan 14.29 0.484 9.81 7.97 13.60 7-Jun-15 Stevens Ck. Blvd. 15.41 0.490 10.53 8.20 8.70 7-Jun-15 Stevens Ck. Blvd. 15.40 0.489 10.91 8.16 8.60 Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 7-Jun-15 West Valley Elem. 16.68 0.507 8.23 8.20 3.40 7-Jun-15 West Valley Elem. 16.69 0.507 8.12 8.20 3.50 7-Jun-15 La Avenida 20.45 0.907 9.75 7.78 1.10 7-Jun-15 La Avenida 20.57 0.905 9.80 7.82 1.60

9-Aug-15 Moss Rock 15.12 787 8.52 7.15 9-Aug-15 Moss Rock 15.14 787 8.41 7.27 9-Aug-15 Moss Rock 15.15 783 8.45 7.46 9-Aug-15 Moss Rock 15.18 784 8.46 7.54 9-Aug-15 Dam Outlet 14.31 556 6.28 6.46 9-Aug-15 Dam Outlet 14.3 531 6.11 6.65 9-Aug-15 Chestnut 14.29 528 6.09 6.75 9-Aug-15 Chestnut 14.29 519 6.05 6.79 9-Aug-15 Chestnut 14.28 519 6.01 6.79 9-Aug-15 McClellan 14.64 516 9.05 7.52 9-Aug-15 McClellan 14.68 512 9.13 7.54 9-Aug-15 Stevens Ck. Blvd. 14.35 512 9.81 7.97 9-Aug-15 Stevens Ck. Blvd. 14.42 511 10.04 7.89 9-Aug-15 West Valley Elem. 15.16 514 10.48 7.85 9-Aug-15 West Valley Elem. 15.16 512 10.46 7.99 9-Aug-15 Evelyn 16.54 509 9.64 8.25 9-Aug-15 Evelyn 16.56 511 9.47 8.27 9-Aug-15 La Avenida 20.49 885 7.85 7.66 9-Aug-15 La Avenida 20.52 886 7.77 7.69

20-Sep-15 Moss Rock 13.15 751 7.41 7.82 0.80 20-Sep-15 Moss Rock 13.15 752 7.19 7.82 0.50 20-Sep-15 Dam Outlet 20.78 464 6.45 7.7 87.20 20-Sep-15 Dam Outlet 20.77 464 7.03 7.58 93.00 20-Sep-15 Chestnut 20.39 469 7.69 7.94 88.20 20-Sep-15 Chestnut 20.42 468 7.83 7.96 87.10 20-Sep-15 McClellan 19.07 464 8.26 8.08 77.20 20-Sep-15 McClellan 19.09 463 8.25 8.12 78.30 20-Sep-15 Stevens Ck. Blvd. 18.69 463 8.7 7.85 67.60 20-Sep-15 Stevens Ck. Blvd. 18.7 465 8.69 7.98 70.00 20-Sep-15 West Valley Elem. 18.06 464 9.13 8.2 64.60 20-Sep-15 West Valley Elem. 18.07 465 9.15 8.26 66.00 20-Sep-15 La Avenida 19.35 728 5.51 7.6 2.60 20-Sep-15 La Avenida 19.35 725 6.39 7.59 2.00

18-Oct-15 Moss Rock 13.11 965 8.17 7.69 0.90 18-Oct-15 Moss Rock 13.15 961 7.44 7.7 0.90 18-Oct-15 Dam Outlet 19.41 646 6.21 7.61 34.90 18-Oct-15 Dam Outlet 19.41 645 6.22 7.66 36.30 18-Oct-15 Chestnut 19.05 598 7.86 7.96 32.90 18-Oct-15 Chestnut 19.06 591 7.77 7.89 31.50 18-Oct-15 McClellan 17.88 578 8.38 8.14 35.20 18-Oct-15 McClellan 17.89 574 8.33 8.14 36.90 Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 18-Oct-15 Stevens Ck. Blvd. 17.3 559 8.98 8.21 49.90 18-Oct-15 Stevens Ck. Blvd. 17.31 559 8.89 8.2 44.50 18-Oct-15 West Valley Elem. 16.61 554 9.53 8.35 27.30 18-Oct-15 La Avenida 18.31 842 6.2 7.58 3.30 18-Oct-15 La Avenida 18.3 843 6.04 7.58 3.30

22-Nov-15 Moss Rock 7.62 856 11.38 7.93 2.30 22-Nov-15 Moss Rock 7.62 859 11.26 7.95 3.50 22-Nov-15 Dam Outlet 12.12 675 7.6 7.83 31.70 22-Nov-15 Dam Outlet 12.12 659 7.65 7.81 29.80 22-Nov-15 Chestnut 11.86 646 9.53 7.93 21.90 22-Nov-15 Chestnut 11.87 646 9.2 7.99 22.20 22-Nov-15 McClellan 10.91 638 10.29 8.11 8.90 22-Nov-15 McClellan 10.91 637 10.15 8.11 9.70 22-Nov-15 Stevens Ck. Blvd. 11.47 641 10.45 8.15 11.70 22-Nov-15 Stevens Ck. Blvd. 11.46 640 10.79 8.14 9.30 22-Nov-15 West Valley Elem. 10.63 638 11.12 8.23 8.10 22-Nov-15 West Valley Elem. 10.63 640 11.06 8.26 8.00 22-Nov-15 La Avenida 16.14 909 6.83 7.58 1.60 22-Nov-15 La Avenida 16.16 908 5.3 7.49 2.90

6-Dec-15 Moss Rock 6.95 704 11.23 8.11 6-Dec-15 Moss Rock 6.96 705 11.27 8.11 6-Dec-15 Dam Outlet 9.03 696 8.15 7.87 6-Dec-15 Dam Outlet 9.03 694 8.14 7.89 6-Dec-15 Chestnut 9.29 664 10.01 8.06 6-Dec-15 Chestnut 9.3 664 10.13 8.06 6-Dec-15 McClellan 8.99 666 10.45 8.17 6-Dec-15 McClellan 9.01 666 10.52 8.17 6-Dec-15 Stevens Ck. Blvd. 9.55 670 10.93 8.24 6-Dec-15 Stevens Ck. Blvd. 9.56 669 10.4 8.23 6-Dec-15 West Valley Elem. 9.15 590 10.56 8.41 6-Dec-15 West Valley Elem. 9.17 662 11.39 8.39 6-Dec-15 Evelyn 9.2 668 11.42 8.38 6-Dec-15 Evelyn 9.2 670 11.29 8.39 6-Dec-15 La Avenida 15.38 925 7.91 7.67 6-Dec-15 La Avenida 15.32 925 8.49 7.71 6-Dec-15 La Avenida 15.3 923 8.57 7.75

17-Jan-16 Moss Rock 9.28 544 11.22 7.95 3.36 17-Jan-16 Moss Rock 9.29 544 11.18 7.95 3.26 17-Jan-16 Dam Outlet 7.54 547 6.22 7.29 64.10 17-Jan-16 Dam Outlet 7.54 539 6.13 7.35 61.50 17-Jan-16 Chestnut 8.21 538 10.25 7.71 37.30 17-Jan-16 Chestnut 8.22 537 10.04 7.72 36.80 17-Jan-16 McClellan 9.07 545 10.07 7.92 22.20 17-Jan-16 McClellan 9.07 551 10.15 7.9 20.10 17-Jan-16 Stevens Ck. Blvd. 9.9 538 10.46 7.97 10.40 Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 17-Jan-16 Stevens Ck. Blvd. 9.9 540 10.44 7.98 9.76 17-Jan-16 West Valley Elem. 10.72 552 10.94 8.2 6.13 17-Jan-16 West Valley Elem. 10.72 552 10.92 8.2 7.29 17-Jan-16 La Avenida 15.09 768 6.98 7.59 3.95 17-Jan-16 La Avenida 15.1 727 6.25 7.56 3.36

28-Feb-16 Moss Rock 8.05 575 12.34 8.2 1.04 28-Feb-16 Moss Rock 8.06 576 12.09 7.89 0.84 28-Feb-16 Dam Outlet 8.45 547 4.35 6.98 23.20 28-Feb-16 Dam Outlet 8.45 545 4.3 7.06 22.90 28-Feb-16 Chestnut 8.96 531 11.48 7.79 21.90 28-Feb-16 Chestnut 8.98 529 11.28 7.83 21.40 28-Feb-16 McClellan 8.9 555 11.48 8.33 18.50 28-Feb-16 McClellan 8.92 555 11.78 8.08 18.00 28-Feb-16 Stevens Ck. Blvd. 9.78 559 12.19 8.15 7.32 28-Feb-16 Stevens Ck. Blvd. 9.79 559 12.24 8.21 7.05 28-Feb-16 West Valley Elem. 10.36 583 12.15 8.36 3.41 28-Feb-16 West Valley Elem. 10.35 586 12.17 8.35 3.51 28-Feb-16 La Avenida 15.68 924 9.46 7.84 2.52 28-Feb-16 La Avenida 15.73 922 9.62 7.79 2.52

20-Mar-16 Moss Rock 9.57 473 11.55 7.97 6.80 20-Mar-16 Moss Rock 9.58 479 11.44 8.02 7.90 20-Mar-16 Dam Outlet 9.87 390 8.61 7.52 62.50 20-Mar-16 Dam Outlet 9.87 387 8.72 7.57 64.60 20-Mar-16 Chestnut 10.08 379 10.36 7.92 57.90 20-Mar-16 Chestnut 10.09 378 10.35 7.87 60.20 20-Mar-16 McClellan 10.41 387 11.53 7.97 50.60 20-Mar-16 McClellan 10.41 390 10.9 7.93 50.40 20-Mar-16 Stevens Ck. Blvd. 10.66 388 10.87 7.97 54.70 20-Mar-16 Stevens Ck. Blvd. 10.67 389 10.84 7.94 56.90 20-Mar-16 West Valley Elem. 10.8 387 11.05 8.09 60.80 20-Mar-16 West Valley Elem. 10.81 388 10.9 8.09 62.40 20-Mar-16 Evelyn 11.53 554 10.92 8.28 49.00 20-Mar-16 Evelyn 11.54 550 10.91 8.27 49.20 20-Mar-16 La Avenida 11.67 572 10.68 8.19 44.20 20-Mar-16 La Avenida 11.68 572 10.64 8.19 43.30

10-Apr-16 Moss Rock 10.64 625 10.83 8.06 11.00 10-Apr-16 Moss Rock 10.64 616 10.83 8.07 15.20 10-Apr-16 Dam Outlet 10.51 416 8.51 7.48 35.00 10-Apr-16 Chestnut 10.59 410 10.25 7.83 30.60 10-Apr-16 Chestnut 10.59 411 10.23 7.82 30.70 10-Apr-16 McClellan 10.75 421 10.6 7.95 26.60 10-Apr-16 McClellan 10.75 422 10.61 7.93 28.50 10-Apr-16 Stevens Ck. Blvd. 10.98 424 10.53 7.95 24.40 10-Apr-16 West Valley Elem. 11.4 403 11.13 8.1 21.70 10-Apr-16 Evelyn 12.73 464 11.75 8.12 Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 10-Apr-16 Evelyn 12.73 465 11.35 8.14 10-Apr-16 La Avenida 13.42 373 10.34 7.83 12.90 10-Apr-16 La Avenida 13.42 407 10.38 7.8 13.80

29-May-16 Moss Rock 11.57 722 11.19 7.69 3.80 29-May-16 Moss Rock 11.59 713 11.34 7.81 4.40 29-May-16 Dam Outlet 12.19 517 9.7 7.17 55.10 29-May-16 Dam Outlet 12.19 502 9.82 7.18 55.20 29-May-16 Chestnut 12.56 495 10.44 7.62 39.30 29-May-16 Chestnut 12.58 495 10.46 7.61 41.50 29-May-16 McClellan 12.59 482 10.78 7.8 33.50 29-May-16 McClellan 12.6 483 10.41 7.74 36.10 29-May-16 Stevens Ck. Blvd. 13.66 493 12.05 8.28 17.80 29-May-16 Stevens Ck. Blvd. 13.7 495 12.28 8.09 28.40 29-May-16 West Valley Elem. 14.46 503 10.62 7.98 17.30 29-May-16 West Valley Elem. 14.41 505 10.85 8.11 17.40 29-May-16 La Avenida 18.32 913 9.43 7.62 1.10 29-May-16 La Avenida 18.75 912 9.3 7.69 1.00

26-Jun-16 Moss Rock 12.84 548.00 11.91 7.74 1.00 26-Jun-16 Moss Rock 12.85 550.00 11.94 7.74 1.40 26-Jun-16 Dam Outlet 13.09 450.00 10.20 6.77 89.80 26-Jun-16 Dam Outlet 13.08 441.00 10.18 6.79 82.60 26-Jun-16 Chestnut 13.33 439.00 11.15 7.75 48.30 26-Jun-16 Chestnut 13.35 441.00 10.99 7.65 49.90 26-Jun-16 McClellan 13.2 445.00 11.22 7.59 39.30 26-Jun-16 McClellan 13.22 442.00 11.27 7.60 38.40 26-Jun-16 Stevens Ck. Blvd. 14.32 459.00 11.95 7.87 27.00 26-Jun-16 Stevens Ck. Blvd. 14.35 460.00 11.84 7.87 25.30 26-Jun-16 West Valley Elem. 15.36 455.00 11.29 8.12 14.90 26-Jun-16 West Valley Elem. 15.37 456.00 11.35 8.01 16.90 26-Jun-16 La Avenida 18.79 808.00 9.77 7.82 3.70 26-Jun-16 La Avenida 18.78 811.00 9.38 7.69 4.50

17-Jul-16 Moss Rock 13.31 734.00 13.34 7.87 2.10 17-Jul-16 Moss Rock 13.34 750.00 13.24 7.99 1.60 17-Jul-16 Dam Outlet 13.92 548.00 13.13 7.18 42.40 17-Jul-16 Dam Outlet 13.92 541.00 13.18 7.17 41.40 17-Jul-16 Chestnut 13.98 534.00 12.72 7.59 51.50 17-Jul-16 McClellan 13.65 524.00 13.17 7.97 35.00 17-Jul-16 McClellan 13.68 525.00 13.09 7.71 32.90 17-Jul-16 Stevens Ck. Blvd. 14.47 539.00 13.78 7.52 25.00 17-Jul-16 Stevens Ck. Blvd. 14.49 540.00 13.84 7.79 22.80 17-Jul-16 West Valley Elem. 15.01 539.00 13.63 8.18 17.60 17-Jul-16 West Valley Elem. 14.96 538.00 13.79 8.16 21.60 17-Jul-16 La Avenida 18.28 933.00 10.98 7.75 2.70 17-Jul-16 La Avenida 18.29 913.00 9.96 7.62 2.00 Stevens Creek Water Chemistry: Sept 2014 - Sept 2016 Temp SpCond DO Conc Turbidity Date Site pH C mS/cm mg/L NTU 14-Aug-16 Moss Rock 13.93 784.00 10.04 7.78 2.00 14-Aug-16 Moss Rock 13.96 783.00 9.95 7.90 1.40 14-Aug-16 Dam Outlet 15.4 587.00 5.72 7.31 37.80 14-Aug-16 Dam Outlet 15.4 576.00 6.30 7.04 38.90 14-Aug-16 Chestnut 15.34 542.00 9.67 7.57 28.50 14-Aug-16 Chestnut 15.35 543.00 9.14 7.53 27.70 14-Aug-16 McClellan 14.78 532.00 10.28 7.89 16.10 14-Aug-16 McClellan 14.79 533.00 10.12 7.88 16.70 14-Aug-16 Stevens Ck. Blvd. 15.39 546.00 10.80 8.03 12.30 14-Aug-16 Stevens Ck. Blvd. 15.41 546.00 11.07 8.08 13.00 14-Aug-16 West Valley Elem. 16.14 549.00 10.68 8.35 6.30 14-Aug-16 West Valley Elem. 16.12 549.00 10.79 8.22 6.10 14-Aug-16 La Avenida 18.31 917.00 8.26 7.79 3.30 14-Aug-16 La Avenida 18.26 913.00 8.27 7.68 3.00 Permanente Creek: Water Chemistry at Fremont Avenue, Sept 2014 ‐ May 2015 Temp SpCond DO Conc Turbidity Date pH C mS/cm mg/L NTU 14‐Sep‐14 dry dry dry dry dry 5‐Oct‐14 dry dry dry dry dry 9‐Nov‐14 dry dry dry dry dry 7‐Dec‐14 dry dry dry dry dry 11‐Jan‐15 12.54 1.087 11.04 8.25 1.2 11‐Jan‐15 12.56 1.083 11.04 8.25 1.2 15‐Feb‐15 14.04 0.859 10.81 7.92 5.5 15‐Feb‐15 14.06 0.861 10.76 7.78 5.8 15‐Mar‐15 15.52 1.164 10.45 8.21 2.8 15‐Mar‐15 15.53 1.168 10.44 8.21 2.6 12‐Apr‐15 13.96 0.977 11.1 8.22 1.3 12‐Apr‐15 13.98 0.979 11.09 8.19 1.4 17‐May‐15 13.82 1.106 10.75 8.33 2.5 17‐May‐15 13.82 1.105 10.77 8.34 2.7

Note: Monitoring was discontinued after May 2015 due to finding New Zealand Mudsnails in Stevens Crk. We had been doing volunteer monitoring of eight sites on Stevens Creek and one site on Permanente Creek at one event a month. We dropped the Permanente site to avoid possible contamination.

Note: We added another Permanente site much lower in the watershed which we monitored with our volunteers in association with the habitat enhancement project we were carrying out along the creek as it runs through the Google campus.

Permanente Creek: Water Chemistry at Amphitheatre Parkway, Oct 2015 ‐ Aug 2016

Temp SpCond DO Conc Turbidity Date pH C mS/cm mg/L NTU 5‐Oct‐15 23.83 42.37 4.97 8.27 31.70 5‐Oct‐15 23.9 42.12 4.76 8.27 30.9 4‐Jan‐16 22.36 1.18 9.70 7.53 19.00 4‐Jan‐16 22.35 1.177 9.01 7.59 20.2 31‐Mar‐16 24.59 1.10 19.18 8.17 17.60 31‐Mar‐16 24.6 1.105 19.5 8.23 18.8 26‐Jun‐16 29.87 1.15 18.62 8.57 23.20 26‐Jun‐16 30.11 1.141 18.65 8.58 33.3 17‐Jul‐16 24.58 3.616 16.35 8.23 38.1 17‐Jul‐16 24.71 3.528 14.15 8.24 26.4 14‐Aug‐16 27.45 1.549 12.46 8.61 21.7 14‐Aug‐16 27.58 1.503 16.31 8.63 22.7 Dissolved Dissolved Dissolved H2O Temp. H20 Temp Conductivit Conductivit Dissolved Dissolved Dissolved Turbidity #1 Turbidity #2 Date Coordinates Waterbody Name Station Name O2 #1 (mg/l O2 #2 (mg/l O2 #3 (mg/l pH #1 pH #2 #1 (◦C) #2 (◦C) y #1 (μS) y #2 (μS) O2 #1 (%) O2 #2 (%) O2 #3 (%) (NTU/JTU) (NTU/JTU) [ppm]) [ppm]) [ppm])

8/15/2014 37.432360, -122.105010 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 22 3700 30.2 2.65 8.06 11.5 8/15/2014 37.371040, -122.118126 Adobe A2: Adobe @ Manresa Lane 18 1325 25 2.37 7.54 6.5 8/15/2014 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 17.9 1010 54.5 5.15 7.84 5.25 8/15/2014 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 19.4 1260 84.2 7.8 8.19 16.4 8/23/2014 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 17.2 1305 32.9 3.15 7.97 7.37 8/23/2014 37.413164, -122.191931 San_Francisquito S2: Los Tranchos @ Piers Lane Bridge 17.5 2050 52 4.97 8.04 2.61 8/23/2014 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 8/23/2014 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 8/23/2014 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 25.4 43,400 58.2 4.78 7.62 269 9/19/2014 37.432360, -122.105010 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 20.3 2,250 96.2 8.69 7.87 38.2 9/19/2014 37.371040, -122.118126 Adobe A2: Adobe @ Manresa Lane 18.2 1078 5.7 0.54 7.04 33.7 9/19/2014 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 18.3 1175 67 6.29 7.84 52.5 9/19/2014 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 17.2 850 54 5.17 7.46 2.15 9/27/2014 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 16.1 1010 14.4 1.41 7.42 10.8 9/27/2014 37.413164, -122.191931 San_Francisquito S2: Los Tranchos @ Piers Lane Bridge 16.6 1980 59.3 5.75 7.91 0.7 9/27/2014 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 9/27/2014 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 9/27/2014 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 18.3 36,020 22.4 2.11 7.43 58.8 10/17/2014 37.432360, -122.105010 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 17.5 1470 72.7 6.95 8.29 13.4 10/17/2014 37.371040, -122.118126 Adobe A2: Adobe @ Manresa Lane 14.2 1295 21.8 2.22 7.47 5 10/17/2014 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 15.6 1400 97.1 9.68 8.22 4.34 10/17/2014 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 14.1 1025 56.2 5.77 7.81 2.45 10/25/2014 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 13.4 1035 20.9 2.18 7.83 3.25 10/25/2014 37.413164, -122.191931 San_Francisquito S2: Los Tranchos @ Piers Lane Bridge 15.3 1930 68.6 6.86 8.05 3.25 10/25/2014 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 18.6 275 77.9 7.27 7.37 55.5 10/25/2014 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 10/25/2014 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 20.4 13,800 77.4 7 7.74 794 11/20/2014 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 16 940 46.9 4.66 7.78 10.9 11/20/2014 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 11/20/2014 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 13.7 1030 28.1 2.91 7.61 4.36 11/20/2014 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 11/21/2014 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 12.5 1035 34.5 3.78 7.53 1.57 11/21/2014 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 13.4 505 74.8 7.84 7.64 22.5 11/21/2014 Deer Creek Deer Creek @ Deer Creek Road 160 48.2 4.96 7.25 57.3 11/21/2014 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 11/21/2014 37.408306, -122.134186 Barron B2: Barron@ Laguna 13.3 320 61.9 6.52 7.3 19 11/15/2014 37.413703, -122.192761 San_Francisquito S1: San Francisquito@ Piers Lane Bridge 11.3 1280 40.8 4.49 7.67 5.15 11/15/2014 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 13 1940 78.3 8.42 8.08 3.78 11/15/2014 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 11/15/2014 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 11/15/2014 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 15.5 25,600 EF EF 7.52 54.1 12/18/2014 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 14.1 1070 100.8 10.34 8.01 1.35 12/18/2014 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 14.1 1035 95.1 9.78 7.96 3.65 12/18/2014 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 12.6 835 99.5 10.64 8.09 4.55 12/18/2014 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 13.3 615 74.7 7.9 7.72 26.9 12/19/2014 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 13.2 805 91.7 9.66 7.84 4.99 12/19/2014 37.408306, -122.134186 Barron B2: Barron@ Laguna 13 710 86.2 9.14 7.67 7.67 12/19/2014 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 13 1270 100.72 10.59 8.03 4.51 12/19/2014 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 13 1310 99.8 10.6 8.08 4.56 12/20/2014 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 12.8 460 98.8 10.52 7.84 36.5 12/20/2014 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 13.2 715 101 10.64 8.03 7.3 12/20/2014 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 12.9 500 99 10.54 7.83 39.1 12/20/2014 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 13.1 470 97.5 10.33 8 40.4 12/20/2014 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 13.2 490 96.9 10.25 7.97 42.9 1/15/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 11.7 732 101.5 11.04 8 1.21 1/15/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 13.1 1000 96.9 10.25 8 1.27 1/15/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 1/15/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 13.4 12 1680 8.4 0.89 7.88 9.88 1/16/2015 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 1/16/2015 37.408306, -122.134186 Barron B2: Barron @ Laguna 1/16/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 9.5 1050 93.1 10.74 7.68 1.18 1/16/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 10.4 945 94.1 10.63 8.01 5.11 1/17/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 9.5 440 97.9 11.28 7.79 1.98 1/17/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 11.3 1300 99.4 10.96 8.11 1.14 1/17/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 9.6 1020 950 98.1 11.3 8.23 7.15 2.13 1/17/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 1/17/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 12.8 15,900 111.5 11.92 7.99 14.6 2/19/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 13.7 740 100.5 10.4 8.2 3.77 2/19/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 14.1 820 97.4 10.02 7.81 5.63 2/19/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 13.3 815 99 10.42 7.77 2.88 2/19/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 15.9 1040 81 8.04 7.35 19 2/20/2015 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 12.7 1055 21.8 2.32 7.22 2.95 2/20/2015 37.408306, -122.134186 Barron B2: Barron@ Laguna 12.7 805 63.4 6.75 7.89 6.81 2/20/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 13 1835 97.2 10.24 8 1.07 2/20/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 13.3 1835 95 9.98 8.48 3.12 2/21/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 11.5 810 97.2 10.54 7.49 5.57 2/21/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 11.3 1035 99.8 10.88 7.94 1.76 2/21/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 12 895 97.8 10.53 7.83 4.71 2/21/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 12.7 875 94.9 10.07 7.13 4.37 2/21/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 14.3 940 109.9 11.26 7.82 3.9 3/19/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 13.6 785 100.5 10.39 7.83 1.3 3/19/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 13.9 850 97.9 10.08 7.64 2.42 3/19/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 3/19/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 17 1290 83.9 8.16 7.93 10.1 3/20/2015 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 3/20/2015 37.408306, -122.134186 Barron B2: Barron @ Laguna 3/20/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 12.2 2075 92.8 9.97 7.98 1.1 3/20/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 13.1 1875 90.9 9.6 8.15 6.01 3/21/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 12.8 960 90.6 9.62 7.42 1.88 3/21/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 12.3 1160 100.2 10.76 8.2 2.61 3/21/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 13.6 105 87.3 9.14 8.36 2.03 3/21/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 3/21/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 16.2 34,500 97.3 9.62 7.46 11.8 4/17/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 16 990 99.8 9.77 7.95 1.21 1.36 4/17/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 14.3 930 95.3 9.73 7.85 1.57 4/17/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 4/17/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 14.8 1185 62.1 6.3 7.76 23.7 4/17/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 12.6 2055 89.7 9.51 7.8 1.84 4/17/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 14 1630 90.7 9.39 7.98 6.2 4/18/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 13.1 1045 83.1 8.65 7.75 2.03 4/18/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 12.8 1240 97.3 10.3 8.3 0.75 4/18/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 4/18/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 4/18/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 16.1 38,100 73.5 7.27 8.77 20 20.6 5/15/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 15.7 895 104.5 10.28 7.92 0.9 0.8 5/15/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 15 915 97.6 9.77 7.78 1.4 1.5 5/15/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 5/15/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 17.3 290 32.3 3.1 7.44 46.8 5/15/2015 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 15.2 700 winkler only 7.69 7.69 30 5/15/2015 37.408306, -122.134186 Barron B2: Barron @ Laguna 15.1 240 winkler only 6.8 7.38 50 5/15/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 15.2 1815 winkler only 7 8.07 10 5/15/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 15 905 winkler only 7 7.75 20 5/23/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 14.9 14.9 1145 1145 85.4 85.3 8.62 8.61 7.72 7.92 1.9 1.8 5/23/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 14.1 14 1340 1340 96.2 96.5 9.9 9.95 8.22 8.26 0.8 5/23/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 5/23/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 5/23/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 17.1 17.1 20,400 41.5 4.01 7.67 51.4 28.5 6/19/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 6/19/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 15.7 910 97.4 9.6 8.19 1.8 6/19/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 6/19/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 19.2 1300 89.1 8.22 8.03 21.6 6/19/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 16.5 2020 55.7 5.44 7.79 1.8 6/19/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 17.3 1340 76 7.3 8.1 9.2 6/20/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 16.5 1360 75.8 7.35 7.92 1.8 6/20/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 16.2 1625 80.5 7.86 8.1 1 6/20/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 20.1 Over 29.8 2.73 7.62 67.8 7/17/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 17.2 830 102.6 9.75 8.29 1.1 7/17/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 20.1 890 96.1 8.65 8.27 2.1 7/17/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 7/17/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 21.4 1250 158.2 13.97 8.14 11.7 7/17/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 18.8 1265 58.8 5.44 7.76 1.5 7/17/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 20.3 1385 78.5 7.05 8.16 2.6 2.7 7/18/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito@ Piers Lane Bridge 18.4 1445 64.9 6.2 7.98 2.9 7/18/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 18.2 1865 64.4 6.06 8.05 1.9 7/18/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito@ El Palo Alto 7/18/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 7/18/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 19.8 37,000 75 6.8 7.65 99.99+ 8/21/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 16.8 880 96.3 9.23 8.28 2.3 8/21/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 19.5 845 92 8.39 8.19 2.3 8/21/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 8/21/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 19.1 1125 79.3 7.3 7.92 3 8/21/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 17.6 855 39.9 3.78 7.6 1.6 8/21/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 19.4 940 34.7 3.21 8.05 2.5 8/21/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge ? 1560 39.1 3.75 7.81 4.8 8/21/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 17.2 1980 70.1 6.73 7.91 2.4 8/21/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 8/21/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 8/21/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 21.8 25,600 107.1 9.39 7.86 99.9 9/18/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 9/18/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 15.8 15.7 1125 1130 89.7 90.5 8.87 8.93 8.22 8.23 9/18/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 9/18/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 21.6 17.9 1730 0.7 47.7 52.8 0.05 4.38 5.08 7.96 9/18/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 15.1 15 1190 1180 48.9 49.6 4.9 4.98 7.69 7.72 9/18/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 17.3 17.2 1605 1585 73 73.5 7 7.1 8.13 8.18 9/26/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 15.1 15.1 1375 1340 40.5 42.7 4.06 4.28 7.8 7.67 9/26/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 15.7 15.7 2,000 2,000 78.9 79 7.8 7.8 7.93 8.08 9/26/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 9/26/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 9/26/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 10/16/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 10/16/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 17.1 17 1020 970 83 85.2 7.95 8.18 7.92 8.07 11.3 13.5 10/16/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 10/16/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 18.6 18.6 1220 1150 65 65 6.06 6.12 7.86 9.3 9.7 10/16/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 17.1 16.8 985 975 37.2 35.2 3.58 3.42 7.49 7.5 3 2.7 10/16/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 18.3 18.3 605 580 91.4 91.4 8.58 8.59 8.08 8.09 48.2 44.7 10/17/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 15.8 15.6 1540 1545 42.8 4 4.17 7.6 7.82 6.2 10/17/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 16.5 16.5 2000 76 75.38 7.38 7.33 8.06 1.6 1 10/17/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 10/17/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 10/17/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 11/20/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 11/20/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 13.1 13 945 955 88.6 89 9.3 9.36 7.94 8.04 1.8 1.4 11/20/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 11/20/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 13.3 13.3 1160 1140 72.4 72.3 7.63 7.62 7.88 7.89 7.5 6 11/19/2015 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 11.5 380 40 4.47 7.33 2.6 11/19/2015 37.408306, -122.134186 Barron B2: Barron @ Los Robles 10.9 305 14 1.57 7.01 3.7 11/19/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 11 1265 24 2.66 7.29 2.5 11/19/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 10.9 1200 82 9.13 7.67 2.9 11/21/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito@ Piers Lane Bridge 10.2 10.2 1015 995 36 4.14 7.64 7.69 3 3 11/21/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 10.7 10.8 1940 1925 84 83.4 9.33 9.4 8.04 8.06 1.9 0.7 11/21/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito@ El Palo Alto 11/21/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito@ University 11/21/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 12.3 12.2 32,900 82.4 83.7 8.88 9.05 7.88 7.87 30.7 30.5 12/18/2015 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 12/18/2015 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 10.7 10.7 980 975 91.6 92 10.16 10.19 8.2 8.19 1.4 1.3 12/18/2015 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 12/18/2015 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 11.3 11.5 1210 1225 78.8 82 8.64 9 7.92 8.06 10.3 10.1 12/17/2015 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 7.4 6.5 5900 5,950 6.58 5.01 9.7 10.1 3.8 3.7 12/17/2015 37.408306, -122.134186 Barron B2: Barron@ Los Robles Avenue Bridge 8.3 7.8 285 290 4.94 4.34 9.98 10.02 3.4 3.4 12/17/2015 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 9 1945 1970 10.76 10.87 1.4 1.3 12/17/2015 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 7.6 7.2 1170 1220 10.71 10.84 8.67 9.05 6.4 5.8 12/19/2015 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 8.7 8.7 933 920 74.34 77.3 8.62 8.87 7.75 7.78 7.7 7.4 12/19/2015 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 10.2 10.2 1830 1860 91 90.7 10.2 10.1 8.08 8.09 2.4 2.2 12/19/2015 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 11.8 12.3 225 200 51.2 61 5.56 6.55 7.66 7.63 36.6 39.2 12/19/2015 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 12/19/2015 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 11.8 11.7 18,100 23,600 76.5 74.8 8.28 8.06 7.84 7.81 38.4 49.5 1/15/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 1/15/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 12.9 12.7 760 745 96.6 96.5 10.23 10.23 8.1 8.19 1.4 1.4 1/15/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 1/15/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 13.7 13.5 815 905 71.9 73 7.63 7.67 7.95 7.97 16.2 15.2 1/14/2016 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 9.7 9.7 390 385 36.5 36.4 4.1 4.15 7.12 7.14 no meter no meter 1/14/2016 37.408306, -122.134186 Barron B2: Barron @ Los Robles Ave Bridge 10.3 10.1 330 335 60.5 59.4 6.83 6.72 7.2 7.2 no meter no meter 1/14/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 10.5 10.4 1185 1190 96.5 96.8 10.83 10.84 7.76 7.78 no meter no meter 1/14/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 10.7 10.7 1240 1255 91.8 92.6 10.25 10.33 7.92 7.95 no meter no meter 1/16/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito@ Piers Lane Bridge 10.7 10.7 530 530 98.7 98.8 10.98 10.99 8.04 8.03 14.4 15 1/16/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 11.8 11.8 655 660 100.8 100.7 10.94 10.93 8.16 8.13 5.2 4.6 1/16/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Park 11.3 11.2 565 545 96.4 98.2 10.63 10.87 8.17 8.14 8.7 8 1/16/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 11.4 11.5 455 455 95.5 95.4 10.51 10.5 8.08 8.05 12.3 12 1/16/2016 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 11.6 11.7 375 380 93.6 96.5 10.27 10.58 8.05 7.99 23.8 20.3 2/18/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 13.3 13.2 660 660 102 102.3 10.64 10.69 8.16 8.23 1.9 1 2/18/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 14.3 14.2 755 735 99 99.3 10.16 10.19 8.24 8.31 1 1.3 2/18/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 2/18/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 12.4 12.4 945 925 68.4 68.2 7.32 7.33 8.11 8.19 2.4 2.4 2/19/2016 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 12.3 12.2 345 330 77 76.7 8.2 8.2 7.4 7.37 24.9 24.2 2/19/2016 37.408306, -122.134186 Barron B2: Barron @ Los Robles Ave Bridge 12.7 12.7 230 200 85 85 9.02 9.02 7.15 7.11 27.1 25.9 2/19/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 12.2 12.2 955 930 98.9 98.8 10.6 10.58 7.94 7.97 6.4 7.7 2/19/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 12.6 12.5 690 675 96.9 96.9 10.3 10.3 7.54 7.64 32.7 37 2/20/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito@ Piers Lane Bridge 11.3 11.3 725 730 98.4 98.5 10.83 10.83 8.2 8.2 5.4 5.1 2/20/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 10.9 10.8 785 785 100.5 100.5 11.17 11.19 8.35 8.38 1.1 2/20/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 11.4 11.4 700 700 100.9 101 11.12 11.13 8.4 8.39 4.5 4.5 2/20/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 11.5 11.5 690 700 99.3 99.3 10.91 10.93 8.4 8.36 3.8 2/20/2016 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 12.1 11.9 700 710 95.9 96 10.39 10.48 8.3 8.3 15.6 15.7 3/18/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 12.7 12.7 565 565 100.8 100.9 10.6 10.63 23.3 21.3 3/18/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 12.9 12.8 600 620 99.2 99.3 10.44 10.46 28.3 25.6 3/18/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 12.7 12.7 595 595 100.2 100.3 10.64 10.65 28.3 26.9 3/18/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 13.3 13.3 670 680 94 93.2 9.88 9.79 22.3 21.4 3/17/2016 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 16.2 16 755 760 58.3 56.3 5.7 5.52 6.15 5.97 3/17/2016 37.408306, -122.134186 Barron B2: Barron @ Los Robles Ave Bridge 14.9 14.5 690 680 85.1 82.2 8.58 8.33 7.42 7.54 3/17/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 14.9 15.4 1255 1280 97.8 97.1 9.8 9.62 6.19 5.86 3/17/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 1310 1265 98 97.9 10.06 10.06 6.58 6.39 3/19/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 12.2 12.2 575 555 99.5 99.3 10.66 10.66 17.5 19.9 3/19/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 11.2 11.2 675 700 102.1 100.1 11.19 11.2 9.4 10 3/19/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 12.5 12.4 600 605 99.5 99.5 10.66 10.65 16.5 17.4 3/19/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 12.7 12.6 600 605 99.2 99.3 10.59 10.59 17.8 16.2 3/19/2016 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 12.9 12.8 590 600 99.3 99.4 10.54 10.56 15.6 15.8 4/15/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 13 13 497.7 498.4 100.5 100.3 10.58 10.54 8.21 8.2 1.3 1.5 4/15/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 13.1 13.1 547 548 96.1 97.2 10.08 10.19 8.21 8.21 1.7 1.6 4/15/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 11.9 11.9 518 524 101.8 101.7 10.96 10.95 8.46 8.45 1.2 1.2 4/15/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 13.1 13 888 890 71.3 72.1 7.51 7.54 8.04 8.04 2.5 2.3 4/15/2016 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 13.3 13.4 315.8 312.6 30.2 29.1 3.15 3 7.4 7.37 11.1 11.2 4/15/2016 37.408306, -122.134186 Barron B2: Barron @ Los Robles Avenue Bridge 14 13.8 265.1 278.6 48 45.7 5.01 4.69 7.61 6.2 6.7 4/15/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 14 14 1226 1243 91.8 90.2 9.4 9.3 8.05 8.06 1.1 1.1 4/15/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 15.2 15.2 1140 1145 95.5 91.9 9.53 9.2 8.23 8.23 1.7 1.8 4/16/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 12.7 12.7 620 622 85.6 90.6 8.92 9.53 8.14 8.13 2 2.9 4/16/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 11.4 11.4 628 628 104.1 103.8 11.33 11.3 8.32 8.32 1.4 1 4/16/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 13 13 656 656 92 93.5 8.37 8.37 8.37 8.37 3 2.2 4/16/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito@ University 14.2 14.2 663 665 91.8 95.8 9.39 9.72 8.27 8.26 2.8 1.9 4/16/2016 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 15.2 15.4 572 675 105.9 110 10.59 10.9 8.32 8.34 2.2 1.9 5/20/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 13.9 13.9 525 521 96.8 96.4 9.97 9.93 8.26 8.26 1 0.9 5/20/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 14 14 571 572 93.4 92.9 9.63 9.56 8.28 8.26 1.6 1.8 5/20/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 5/20/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 19.2 19.3 1221 1228 79.4 78 7.31 7.18 8.02 8.03 4.6 5.3 5/19/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 16.6 16.1 1605 1648 69.4 70 6.67 6.83 7.87 7.86 1.4 1.2 5/19/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 18.4 18.4 1368 1363 93.6 92.5 8.77 8.61 8.27 8.3 2 2.8 5/28/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 16.3 16.3 793 792 53.8 67.8 6.3 6.7 7.86 7.89 1.8 2 5/28/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 14.9 14.9 857 857 95.4 94.1 9.6 9.47 8.15 8.15 0.7 0.7 5/28/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 17.5 17.9 822 830 64 63.1 6.1 6.6 8.03 8.02 2.1 2 5/28/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 5/28/2016 37.453075, -122.127672 San_Francisquito S5: San Francisquito @ East Bayshore Road 6/17/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 15.7 15.7 595 595 113.4 107.1 11.27 10.67 8.27 8.28 1.5 0.9 6/17/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 15.7 15.7 658 656 88.1 92.4 8.74 9.19 8.19 8.2 2.3 2.8 6/17/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 6/17/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 19.7 19.7 1169 1176 92.5 94.3 8.54 8.6 7.88 7.88 8.7 8 6/27/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 17 17 1685 1733 38.6 48.3 3.7 4.58 7.69 7.73 2.1 1.3 6/27/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 18.7 18.7 1112 1113 83.4 67.3 7.74 6.25 7.95 8.07 1.4 1.5 6/18/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 15.8 15.8 965 965 83.7 82.9 8.43 8.18 7.88 7.89 1.2 1.3 6/18/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 14.7 14.7 943 943 100.3 99.2 10.12 10.01 8.18 8.18 0.8 0.7 6/18/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 6/18/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 7/15/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 16.85 16.65 590 591 101.05 96 9.715 9.31 8.28 8.28 1.4 1 7/15/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 16.95 16.65 656 660 96.8 91.45 9.31 8.94 8.27 8.27 3.3 3.1 7/15/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 7/15/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 19.1 19 1014 1014 74.05 73.6 6.85 6.775 7.99 7.99 8.5 7.6 7/13/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 17.05 16.6 1488 1420 50.95 56.95 4.92 5.5 7.69 7.68 2.5 3.8 7/13/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 18.55 18.65 986 987 84.95 114 7.975 10.69 8.18 8.22 2.3 2.3 7/16/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 17.7 18 1162 1171 60.05 49.52 5.845 4.44 7.85 7.92 1.6 1.5 7/16/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 17.21 17.2 81.6 85.4 7.82 8.15 8.16 8.15 0.9 0.9 7/16/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto Park 7/16/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University 8/19/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 16.6 16.6 575 575 99.1 98.9 9.66 9.6 8.38 8.38 1.1 1 8/19/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 17.3 17.3 679 679 92.3 93.6 8.85 8.93 8.28 8.28 1.9 1.3 8/19/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 8/19/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 20.1 20.1 976 977 130.8 141.4 11.84 12.75 8.09 8.11 9.5 11.1 8/17/2016 37.408306, -122.134186 Barron B2: Barron @ Los Robles Ave Bridge 8/17/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 17.8 18 876 880 66.4 63.5 6.34 6.03 7.77 7.75 1.9 1.7 8/17/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 20.1 20.2 1,131 1,134 102.7 94.5 8.38 8.53 8.45 8.46 1.7 1.7 8/20/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 17.6 17.6 1207 1206 63.4 63.2 5.96 5.86 7.89 7.91 2.6 2.6 8/20/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 17.3 17.3 1463 1464 71.5 72.3 6.91 8.08 8.08 0.7 0.8 8/20/2016 37.447369, -122.170222 San_Francisquito S3: San Francisquito @ El Palo Alto 8/26/2016 37.457881, -122.142161 San_Francisquito S4: San Francisquito @ University Ave Bridge 9/16/2016 37.360617, -122.125231 Adobe A1: Adobe @ Foothill College Pedestrian Bridge 15.1 15.1 552 552 97 96.9 9.75 9.74 8.31 8.31 1 1.6 9/16/2016 37.371058, -122.118075 Adobe A2: Adobe @ Manresa Lane 15.6 15.6 670 671 90 92.6 8.86 9.18 8.18 8.18 1.6 3.3 9/16/2016 37.401347, -122.124494 Adobe A3: Adobe @ Terman Middle School 9/16/2016 37.432336, -122.105058 Adobe A4: Adobe @ East Bayshore Road Pedestrian Bridge 18.8 18.8 961 960 82.8 86.2 7.68 8 7.85 7.93 31.9 31.3 9/14/2016 37.393983, -122.142942 Barron B1: Barron @ Ohlone Way 9/14/2016 37.408306, -122.134186 Barron B2: Barron @ Los Robles Avenue Bridge 9/14/2016 37.404014, -122.15765 Matadero M1: Matadero @ Old Page Mill Road 14.5 14.5 768 769 46.1 45 4.69 4.52 7.6 7.59 2.3 2.3 9/14/2016 37.411106, -122.138717 Matadero M2: Matadero @ Bol Park 16.4 16.2 977 973 83.1 83.3 8.1 8.14 8.16 8.26 1.7 1 9/10/2016 37.413703, -122.192761 San_Francisquito S1: San Francisquito @ Piers Lane Bridge 15.9 16 1166 1174 35.5 52.5 44.4 3.49 5.04 4.22 7.73 7.82 2 2.3 9/10/2016 37.413164, -122.191931 San_Francisquito S2: Los Trancos @ Piers Lane Bridge 16.1 16.1 1515 1511 82 81.3 8.01 7.94 8.09 8.1 1 0.8 BMI Site Data 2013

Wetted Width ft. Canopy Cover % Covered Site Date/Time Lat Long Water Temp Spec. Cond. DO %DO mg/L pH Turbidity Upper Middle Lower Upper Middle Lower

Dam 28 Sept. 2013, 9:53am 37.3007 -122.07408 18.33 18.33 597 597 85.4 85.4 8.02 8.02 8.09 8.09 24.9 24.7 9.1 14.3 10.6 60 70 80 Chestnut 28 Sept. 2013, 3:21pm 37.3056 -122.07195 18.33 18.33 594 596 89.2 89 8.38 8.35 8.13 8.12 20 20.9 23.2 20.9 17.5 85 85 90 McClellan 29 Sept. 2013, 9:27am 37.31412 -122.06451 14.58 14.59 598 598 94.1 93.4 9.53 9.49 8.13 7.84 12 12.1 16.8 15 14.4 70 70 40 Blackberry 29 Sept. 2013, 12:18pm 37.31566 -122.06124 15.23 15.25 0.597 0.597 98.3 97.6 9.85 9.75 7.94 7.88 10.2 10.4 4.6 8.6 11.1 90 90 50 West Valley School 29 Sept. 2013, 2:09pm 37.341741 -122.06481 15.12 15.11 0.638 0.638 95.8 95.4 9.62 9.53 8.25 8.25 3.9 4.1 18 16.6 17.2 70 90 85

Depth ft. Five repicate sites Velocity ft/sec Five repicate sites Site 12 3 4 5 12345

Dam 0.3 0.3 0.3 0.4 0.3 0.4 0.4 0.3 0.2 0.3 2.1 1.5 1 1.3 0.6 0.4 0.7 0.9 0.7 1.6 Chestnut 0.4 0.4 0.3 0.3 0.4 0.5 0.4 0.4 0.3 0.3 1.6 0.6 0.8 1.1 1.4 1.7 0.8 0.5 0 1.4 McClellan 0.35 0.3 0.35 0.3 0.3 0.3 0.35 0.35 0.4 0.4 0.7 0.9 0.5 1.3 0.8 0.4 0.9 0.5 0.5 0.2 Blackberry 0.7 0.6 0.6 0.4 0.4 0.4 0.6 0.6 0.6 0.5 1.2 1.4 0.5 0.4 0.2 1.2 0.5 1 1.2 0.9 West Valley School 0.4 0.3 0.3 0.3 0.25 0.25 0.35 0.3 0.35 0.35 0.9 0.2 0.4 1 0.2 0.3 0.4 0.6 0.9 0.8

Major Particle Size mm (B axis. Six largest particles in each of five replicate sites

Site 12 34 5

Dam 52 85 53 40 36 120 45 60 29 38 41 126 55 39 47 54 40 54 98 56 43 101 93 41 220 69 58 46 45 136 Chestnut 82 77 72 65 53 65 124 89 88 57 66 66 127 124 145 91 76 77 142 88 62 59 50 43 56 133 114 87 72 62 McClellan 74 76 59 66 34 35 47 55 83 45 62 52 65 64 62 68 64 98 43 95 80 49 65 98 110 55 51 62 56 64 Blackberry 58 48 94 87 160 120 29 38 27 30 33 85 42 89 82 135 72 88 61 44 95 68 71 140 108 68 62 53 122 108 West Valley School 79 58 76 55 91 115 58 60 54 73 44 45 51 47 39 60 98 118 102 68 57 87 102 75 85 58 53 42 79 61 BMI sample 2013 DA1 DA2 DA3 DA4 DA5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra - - - - - Fla tw orms - - - - - Tricladida Planariidae flatworm 8 8 3 2 5 1 1 4 4 1 1 Proboscis worms - - - - - (Nemertea) Hoplonemertea proboscis worm - 1 1 1 1 - - Nematodes - - - - - (Nematoda) Mermithidae nematode - - - - - Horsehair worms - - - - - (Gordioidea) Gordiidae horsehair worm - - - - - Bryozoans - - - - - (Bryozoa) Plumatellidae bryozoan, moss animals - - - - - Annelid worms - - - - - (Polychaeta) Nereididae polychaete - - - - - (Oligochaeta) (Microdrili) microdrile worm 2 1 3 6 6 18 11 29 10 5 15 - (Oligochaeta) (Megadrili) earthworm - - - - - (Hirudinea) Glossiphoniidae leech - - - - - Crustaceans - - - - - (Cladocera) water flea - - - - - (Ostracoda) ostracod, seed shrimp - - - - - (Eucopepoda) copepod - - - - - Mysidacea Mysididae opossum shrimp - - - - - (Isopoda) isopod, sowbug - - - - - Amphipoda Corophiidae Corophium - - - - - Amphipoda Crangonyctidae amphipod - - - - - Amphipoda Hyalellidae amphipod 1 1 6 6 24 1 25 18 2 20 14 14 Decopoda Astacidae signal crayfish - - - - - Decopoda Cambaridae crawfish - - - - - Decopoda Grapsidae mitten crab - - - - - Insects - - - - - Ephemeroptera Ameletidae primitive minnow mayfly - - - - - Ephemeroptera Siphlonuridae primitive minnow mayfly - - - - - Ephemeroptera Baetidae small minnow mayfly 3 3 1 2 3 - - 2 2 Ephemeroptera Heptageniidae flatheaded mayfly - - - - - Ephemeroptera Ephemerellidae spiny crawler mayfly - - - - - Ephemeroptera Tricorythidae little stout crawler mayfly - - - - - Ephemeroptera Caenidae squaregill mayfly - - - - - Ephemeroptera Leptophlebiidae pronggill mayfly - - - - - Odonata Aeshnidae darner (dragonfly) - - - - - Odonata Cordulegastridae biddy (dragonfly) - - - - - Odonata Gomphidae clubtail (dragonfly) - - - - - Odonata Libellulidae skimmer (dragonfly) - - - - - Odonata Calopterygidae broadwinged damselfly - - - - - Odonata Lestidae spreadwinged damselfly - - - - - Odonata Coenagrionidae narrowwinged damselfly 4 4 3 3 4 4 2 2 1 1 Plecoptera Pteronarcyidae giant stonefly - - - - - Plecoptera Peltoperlidae roachlike stonefly - - - - - Plecoptera Nemouridae broadback stonefly 11 3 14 1 1 1 1 - - Plecoptera Leuctridae rolledwinged stonefly - - - - - Plecoptera Capniidae slender winter stonefly - - - - - Plecoptera Perlidae common stonefly - - - - - Plecoptera Perlodidae perlodid stonefly - - - - - Plecoptera Chloroperlidae green stonefly - - - - - Hemiptera Belostomatidae giant water bug - - - - - Hemiptera Corixidae water boatman - - - - - Megaloptera Sialidae alderfly 2 2 - 4 4 - 1 1 Megaloptera Corydalidae fishfly - 4 4 ------Gerriadae water strider - - - - - Hemiptera Veliidae ------major group/ order (other) family (other) common name - - - - - Insects - - - - - Trichoptera Philopotamidae fingernet caddisfly - - - - - Trichoptera Psychomyiidae nettube caddisfly - - - - - Trichoptera Polycentropodidae tubenet caddisfly - - - - - Trichoptera Hydropsychidae common netspinner 110 2 112 255 9 264 18 1 19 16 16 38 38 Trichoptera Rhyacophilidae freeliving caddisfly - - - - - Trichoptera Glossosomatidae saddlecase caddisfly - - - - - Trichoptera Hydroptilidae micro caddisfly 1 1 - 4 4 6 6 1 1 Trichoptera Brachycentridae humpless cased caddisfly - - - - - Trichoptera Lepidostomatidae lepidostomatid cased caddisfly - - 1 1 - - Trichoptera Limnephilidae northern cased caddisfly - - - - - Trichoptera Uenoidae (northern cased caddisfly) - - - - - Trichoptera Sericostomatidae bushtailed cased caddisfly - - - - - Trichoptera Odontoceridae strongcase caddisfly - - - - - Trichoptera Leptoceridae longhorned cased caddisfly - - - - - Lepidoptera Pyralidae aquatic pyralid moth - - - - - Coleoptera Gyrinidae whirligig beetle - - - - - Coleoptera Haliplidae crawling water beetle - - - - - Coleoptera Dytiscidae predaceous diving beetle - - - - - Coleoptera Hydrophilidae water scavenger beetle - - - - - Coleoptera Hydraenidae minute moss beetle - - - - - Coleoptera Psephenidae water penny - - - - - Coleoptera Dryopidae longtoed water beetle - - - - - Coleoptera Scirtidae marsh beetle - - - - - Coleoptera Elmidae riffle beetle - - - - - Diptera Blephariceridae netwing midge, button sucker - - - - - Diptera Tipulidae crane fly - 9 1 10 - 1 1 8 8 Diptera Psychodidae moth fly - - - - - Diptera Ceratopogonidae biting midge - - - - - Diptera Simuliidae black fly 175 6 181 40 40 3 3 - 5 5 Diptera Chironomidae chironomid midge 34 19 53 139 23 162 326 91 417 200 31 231 84 84 Diptera Chaoboridae phantom midge - - 1 1 - - Diptera Dixidae dixid midge - - - - - Diptera Stratiomyidae soldier fly - - - - - Diptera Tabanidae horse or deer fly - - - - - Diptera Pelecorhynchidae - - - - - Diptera Dolichopodidae longlegged fly - - - - - Diptera Empididae dance fly 1 1 4 4 8 4 1 5 4 4 10 10 Diptera Syrphidae syrphid, rattailed maggot - - - - - Diptera Ephydridae shore fly - - - - - Diptera Sciomyzidae - - - - - Diptera Muscidae muscid - - - - - Mites - - - - - (Acari) (Acari) mite 1 1 - - - - Mollusks - - - - - (Gastropoda) Hydrobiidae gilled snails - - - - - (Gastropoda) Physidae left-handed snail - - - 1 1 - (Gastropoda) Lymnaeidae pond snail - - - - - (Gastropoda) Planorbidae ramshorn snail 1 1 - - - - (Bivalvia) Corbiculidae Asian clam 1 1 - - - - (Bivalvia) Sphaeriidae pea or fingernail clam - - 1 1 - - Ancylidae - - - - - Planorbidae- Ferissa - - - - - Gastropoda Ancylidae - - - - - Lepidoptera Nepticulidae Leaf mining moth - - - - - Hemiptera Veliidae - - - - - BMI sample 2013 CH1 CH2 CH3 CH4 CH5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra - - - - - Fla tw or m s - - - - - Tricladida Planariidae flatworm - - - - - Proboscis worms - - - - - (Nemertea) Hoplonemertea proboscis worm - - 1 1 1 1 - Nematodes - - - - - (Nematoda) Mermithidae nematode - 11 11 2 2 1 1 4 4 Horsehair worms - - - - - (Gordioidea) Gordiidae horsehair worm - - - - - Bryozoans - - - - - (Bryozoa) Plumatellidae bryozoan, moss animals - - - - - Annelid worms - - - - - (Polychaeta) Nereididae polychaete - - - - - (Oligochaeta) (Microdrili) microdrile worm - 12 12 1 1 1 5 6 3 3 (Oligochaeta) (Megadrili) earthworm 1 1 - - - 2 2 (Hirudinea) Glossiphoniidae leech - - - - - Crustaceans - - - - - (Cladocera) water flea - - - - - (Ostracoda) ostracod, seed shrimp - - - - - (Eucopepoda) copepod - - - - - Mysidacea Mysididae opossum shrimp - - - - - (Isopoda) isopod, sowbug - - - - - Amphipoda Corophiidae Corophium - - - - - Amphipoda Crangonyctidae amphipod 9 9 5 5 2 2 3 3 12 12 Amphipoda Hyalellidae amphipod - - - - - Decopoda Astacidae signal crayfish - - - - - Decopoda Cambaridae crawfish - - - - - Decopoda Grapsidae mitten crab - - - - - Insects - - - - - Ephemeroptera Ameletidae primitive minnow mayfly - - - - - Ephemeroptera Siphlonuridae primitive minnow mayfly - - - - - Ephemeroptera Baetidae small minnow mayfly 9 3 12 11 11 37 10 47 10 10 8 8 Ephemeroptera Heptageniidae flatheaded mayfly - - - - - Ephemeroptera Ephemerellidae spiny crawler mayfly - - - - - Ephemeroptera Tricorythidae little stout crawler mayfly 1 1 - - 1 1 - Ephemeroptera Caenidae squaregill mayfly - - - - - Ephemeroptera Leptophlebiidae pronggill mayfly - - - - - Odonata Aeshnidae darner (dragonfly) - - - - - Odonata Cordulegastridae biddy (dragonfly) - - - - - Odonata Gomphidae clubtail (dragonfly) - - - - - Odonata Libellulidae skimmer (dragonfly) - - - - - Odonata Calopterygidae broadwinged damselfly - - - - - Odonata Lestidae spreadwinged damselfly - - - - - Odonata Coenagrionidae narrowwinged damselfly - 1 1 1 1 1 1 2 2 Plecoptera Pteronarcyidae giant stonefly - - - - - Plecoptera Peltoperlidae roachlike stonefly - - - - - Plecoptera Nemouridae broadback stonefly 19 2 21 5 5 14 3 17 3 3 6 6 Plecoptera Leuctridae rolledwinged stonefly - - - - - Plecoptera Capniidae slender winter stonefly - - - - - Plecoptera Perlidae common stonefly - - - - - Plecoptera Perlodidae perlodid stonefly 3 3 - 1 1 4 4 - Plecoptera Chloroperlidae green stonefly - - - - - Hemiptera Belostomatidae giant water bug - - - - - Hemiptera Corixidae water boatman - - - - - Megaloptera Sialidae alderfly 5 5 7 7 3 3 4 4 10 10 Megaloptera Corydalidae fishfly ------Gerriadae water strider - - - - - Hemiptera Veliidae ------major group/ order (other) family (other) common name - - - - - Insects - - - - - Trichoptera Philopotamidae fingernet caddisfly - - - - - Trichoptera Psychomyiidae nettube caddisfly - - - - - Trichoptera Polycentropodidae tubenet caddisfly - - - - - Trichoptera Hydropsychidae common netspinner 57 5 62 11 1 12 146 32 178 49 49 31 3 34 Trichoptera Rhyacophilidae freeliving caddisfly - - - - - Trichoptera Glossosomatidae saddlecase caddisfly - - - - - Trichoptera Hydroptilidae micro caddisfly - - - - - Trichoptera Brachycentridae humpless cased caddisfly - - - - 1 1 Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 2 2 - - - - Trichoptera Limnephilidae northern cased caddisfly - - - - - Trichoptera Uenoidae (northern cased caddisfly) - - - - 1 1 Trichoptera Sericostomatidae bushtailed cased caddisfly - - - - - Trichoptera Odontoceridae strongcase caddisfly - - - - - Trichoptera Leptoceridae longhorned cased caddisfly - - - - - Lepidoptera Pyralidae aquatic pyralid moth - - - - - Coleoptera Gyrinidae whirligig beetle - - - - - Coleoptera Haliplidae crawling water beetle - - - - - Coleoptera Dytiscidae predaceous diving beetle - - - - - Coleoptera Hydrophilidae water scavenger beetle - - - - - Coleoptera Hydraenidae minute moss beetle - - - - - Coleoptera Psephenidae water penny - - - - - Coleoptera Dryopidae longtoed water beetle - - - - - Coleoptera Scirtidae marsh beetle - - - - - Coleoptera Elmidae riffle beetle - - 1 1 - - Diptera Blephariceridae netwing midge, button sucker - - - - - Diptera Tipulidae crane fly - - 1 1 2 1 1 2 2 Diptera Psychodidae moth fly - - - - - Diptera Ceratopogonidae biting midge - - - - - Diptera Simuliidae black fly - 1 1 26 4 30 2 2 - Diptera Chironomidae chironomid midge 54 40 94 50 50 21 11 32 34 1 35 103 3 106 Diptera Chaoboridae phantom midge - - - - - Diptera Dixidae dixid midge - - - - - Diptera Stratiomyidae soldier fly - - - - - Diptera Tabanidae horse or deer fly - - - - - Diptera Pelecorhynchidae - - - - - Diptera Dolichopodidae longlegged fly - - - - - Diptera Empididae dance fly 1 1 - - - 1 1 Diptera Syrphidae syrphid, rattailed maggot - - - - - Diptera Ephydridae shore fly - - - - - Diptera Sciomyzidae - - - - - Diptera Muscidae muscid - - - - - Mites - - - - - (Acari) (Acari) mite - 6 6 - - 8 8 Mollusks - - - - - (Gastropoda) Hydrobiidae gilled snails - - - - - (Gastropoda) Physidae left-handed snail - - - - - (Gastropoda) Lymnaeidae pond snail - - - - - (Gastropoda) Planorbidae ramshorn snail - - - - - (Bivalvia) Corbiculidae Asian clam - - - - - (Bivalvia) Sphaeriidae pea or fingernail clam - - - - - Ancylidae - - - - - Planorbidae- Ferissa - - - - - Gastropoda Ancylidae - - - - - Lepidoptera Nepticulidae Leaf mining moth - - - - - Hemiptera Veliidae - - - - - BMI sample 2013 MC1 MC2 MC3 MC4 MC5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra Fla tw or m s Tricladida Planariidae flatworm 18 1 6 8 1 8 3 Proboscis worms (Nemertea) Hoplonemertea proboscis worm Nematodes (Nematoda) Mermithidae nematode 12 Horsehair worms (Gordioidea) Gordiidae horsehair worm Bryozoans (Bryozoa) Plumatellidae bryozoan, moss animals Annelid worms (Polychaeta) Nereididae polychaete (Oligochaeta) (Microdrili) microdrile worm 1 81 (Oligochaeta) (Megadrili) earthworm 161194041414 (Hirudinea) Glossiphoniidae leech Crustaceans (Cladocera) water flea (Ostracoda) ostracod, seed shrimp (Eucopepoda) copepod Mysidacea Mysididae opossum shrimp (Isopoda) isopod, sowbug Amphipoda Corophiidae Corophium Amphipoda Crangonyctidae amphipod 2 Amphipoda Hyalellidae amphipod 3 Decopoda Astacidae signal crayfish Decopoda Cambaridae crawfish Decopoda Grapsidae mitten crab Insects Ephemeroptera Ameletidae primitive minnow mayfly Ephemeroptera Siphlonuridae primitive minnow mayfly Ephemeroptera Baetidae small minnow mayfly 30 1 37 7 56 1 35 8 17 1 Ephemeroptera Heptageniidae flatheaded mayfly Ephemeroptera Ephemerellidae spiny crawler mayfly Ephemeroptera Tricorythidae little stout crawler mayfly 11 13 1 4 10 1 19 1 Ephemeroptera Caenidae squaregill mayfly Ephemeroptera Leptophlebiidae pronggill mayfly Odonata Aeshnidae darner (dragonfly) Odonata Cordulegastridae biddy (dragonfly) Odonata Gomphidae clubtail (dragonfly) Odonata Libellulidae skimmer (dragonfly) Odonata Calopterygidae broadwinged damselfly Odonata Lestidae spreadwinged damselfly Odonata Coenagrionidae narrowwinged damselfly 11 Plecoptera Pteronarcyidae giant stonefly Plecoptera Peltoperlidae roachlike stonefly Plecoptera Nemouridae broadback stonefly 8 133281431 Plecoptera Leuctridae rolledwinged stonefly Plecoptera Capniidae slender winter stonefly Plecoptera Perlidae common stonefly Plecoptera Perlodidae perlodid stonefly 21111 Plecoptera Chloroperlidae green stonefly Hemiptera Belostomatidae giant water bug Hemiptera Corixidae water boatman Megaloptera Sialidae alderfly 131523 Megaloptera Corydalidae fishfly

Gerriadae water strider Hemiptera Veliidae

major group/ order (other) family (other) common name Insects Trichoptera Philopotamidae fingernet caddisfly Trichoptera Psychomyiidae nettube caddisfly Trichoptera Polycentropodidae tubenet caddisfly Trichoptera Hydropsychidae common netspinner 246 3 198 12 362 9 216 7 98 4 Trichoptera Rhyacophilidae freeliving caddisfly Trichoptera Glossosomatidae saddlecase caddisfly 112 Trichoptera Hydroptilidae micro caddisfly Trichoptera Brachycentridae humpless cased caddisfly Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 30 1 Trichoptera Limnephilidae northern cased caddisfly Trichoptera Uenoidae (northern cased caddisfly) Trichoptera Sericostomatidae bushtailed cased caddisfly Trichoptera Odontoceridae strongcase caddisfly Trichoptera Leptoceridae longhorned cased caddisfly Lepidoptera Pyralidae aquatic pyralid moth Coleoptera Gyrinidae whirligig beetle Coleoptera Haliplidae crawling water beetle Coleoptera Dytiscidae predaceous diving beetle Coleoptera Hydrophilidae water scavenger beetle Coleoptera Hydraenidae minute moss beetle Coleoptera Psephenidae water penny Coleoptera Dryopidae longtoed water beetle Coleoptera Scirtidae marsh beetle Coleoptera Elmidae riffle beetle 12 1 29 55 3 24 1 8 1 Diptera Blephariceridae netwing midge, button sucker Diptera Tipulidae crane fly 12 82 6 345 6 Diptera Psychodidae moth fly Diptera Ceratopogonidae biting midge 1 Diptera Simuliidae black fly 72361119 Diptera Chironomidae chironomid midge 19 8 1 17 317 9 41 1 Diptera Chaoboridae phantom midge Diptera Dixidae dixid midge Diptera Stratiomyidae soldier fly Diptera Tabanidae horse or deer fly Diptera Pelecorhynchidae Diptera Dolichopodidae longlegged fly Diptera Empididae dance fly Diptera Syrphidae syrphid, rattailed maggot Diptera Ephydridae shore fly Diptera Sciomyzidae Diptera Muscidae muscid Mites (Acari) (Acari) mite 11 Mollusks (Gastropoda) Hydrobiidae gilled snails (Gastropoda) Physidae left-handed snail (Gastropoda) Lymnaeidae pond snail (Gastropoda) Planorbidae ramshorn snail (Bivalvia) Corbiculidae Asian clam 15233 (Bivalvia) Sphaeriidae pea or fingernail clam Ancylidae 1 Planorbidae- Ferissa Gastropoda Ancylidae Lepidoptera Nepticulidae Leaf mining moth Hemiptera Veliidae BMI sample 2013 BB 1 BB2 BB3 BB 4 BB 5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra Fla tw or m s Tricladida Planariidae flatworm 1 Proboscis worms (Nemertea) Hoplonemertea proboscis worm Nematodes (Nematoda) Mermithidae nematode 311 Horsehair worms (Gordioidea) Gordiidae horsehair worm Bryozoans (Bryozoa) Plumatellidae bryozoan, moss animals Annelid worms (Polychaeta) Nereididae polychaete (Oligochaeta) (Microdrili) microdrile worm 11 2 20 4 1 (Oligochaeta) (Megadrili) earthworm 31 (Hirudinea) Glossiphoniidae leech Crustaceans (Cladocera) water flea (Ostracoda) ostracod, seed shrimp (Eucopepoda) copepod Mysidacea Mysididae opossum shrimp (Isopoda) isopod, sowbug Amphipoda Corophiidae Corophium Amphipoda Crangonyctidae amphipod 341 Amphipoda Hyalellidae amphipod Decopoda Astacidae signal crayfish Decopoda Cambaridae crawfish Decopoda Grapsidae mitten crab Insects Ephemeroptera Ameletidae primitive minnow mayfly Ephemeroptera Siphlonuridae primitive minnow mayfly Ephemeroptera Baetidae small minnow mayfly 35 2 820 241 Ephemeroptera Heptageniidae flatheaded mayfly Ephemeroptera Ephemerellidae spiny crawler mayfly Ephemeroptera Tricorythidae little stout crawler mayfly 4 2211 Ephemeroptera Caenidae squaregill mayfly Ephemeroptera Leptophlebiidae pronggill mayfly Odonata Aeshnidae darner (dragonfly) Odonata Cordulegastridae biddy (dragonfly) Odonata Gomphidae clubtail (dragonfly) Odonata Libellulidae skimmer (dragonfly) Odonata Calopterygidae broadwinged damselfly Odonata Lestidae spreadwinged damselfly Odonata Coenagrionidae narrowwinged damselfly 24 1 Plecoptera Pteronarcyidae giant stonefly Plecoptera Peltoperlidae roachlike stonefly Plecoptera Nemouridae broadback stonefly 313216 Plecoptera Leuctridae rolledwinged stonefly Plecoptera Capniidae slender winter stonefly Plecoptera Perlidae common stonefly Plecoptera Perlodidae perlodid stonefly Plecoptera Chloroperlidae green stonefly Hemiptera Belostomatidae giant water bug Hemiptera Corixidae water boatman Megaloptera Sialidae alderfly 3 951 Megaloptera Corydalidae fishfly

Gerriadae water strider Hemiptera Veliidae

major group/ order (other) family (other) common name Insects Trichoptera Philopotamidae fingernet caddisfly Trichoptera Psychomyiidae nettube caddisfly Trichoptera Polycentropodidae tubenet caddisfly Trichoptera Hydropsychidae common netspinner 39219842038 Trichoptera Rhyacophilidae freeliving caddisfly Trichoptera Glossosomatidae saddlecase caddisfly Trichoptera Hydroptilidae micro caddisfly 12 Trichoptera Brachycentridae humpless cased caddisfly Trichoptera Lepidostomatidae lepidostomatid cased caddisfly Trichoptera Limnephilidae northern cased caddisfly Trichoptera Uenoidae (northern cased caddisfly) Trichoptera Sericostomatidae bushtailed cased caddisfly Trichoptera Odontoceridae strongcase caddisfly Trichoptera Leptoceridae longhorned cased caddisfly Lepidoptera Pyralidae aquatic pyralid moth Coleoptera Gyrinidae whirligig beetle Coleoptera Haliplidae crawling water beetle Coleoptera Dytiscidae predaceous diving beetle Coleoptera Hydrophilidae water scavenger beetle Coleoptera Hydraenidae minute moss beetle Coleoptera Psephenidae water penny Coleoptera Dryopidae longtoed water beetle Coleoptera Scirtidae marsh beetle Coleoptera Elmidae riffle beetle 13 1 36332 Diptera Blephariceridae netwing midge, button sucker Diptera Tipulidae crane fly 4 331310 Diptera Psychodidae moth fly Diptera Ceratopogonidae biting midge Diptera Simuliidae black fly 71 1 Diptera Chironomidae chironomid midge 10 3 3 125 91 8 Diptera Chaoboridae phantom midge Diptera Dixidae dixid midge Diptera Stratiomyidae soldier fly Diptera Tabanidae horse or deer fly Diptera Pelecorhynchidae Diptera Dolichopodidae longlegged fly Diptera Empididae dance fly Diptera Syrphidae syrphid, rattailed maggot 1 Diptera Ephydridae shore fly Diptera Sciomyzidae Diptera Muscidae muscid Mites (Acari) (Acari) mite 1 1 Mollusks (Gastropoda) Hydrobiidae gilled snails (Gastropoda) Physidae left-handed snail (Gastropoda) Lymnaeidae pond snail (Gastropoda) Planorbidae ramshorn snail (Bivalvia) Corbiculidae Asian clam (Bivalvia) Sphaeriidae pea or fingernail clam 1 Ancylidae Planorbidae- Ferissa Gastropoda Ancylidae Lepidoptera Nepticulidae Leaf mining moth Hemiptera Veliidae BMI sample 2013 WV1 WV2 WV3 WV4 WV5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra Fla tw or m s Tricladida Planariidae flatworm 51415 5 211 Proboscis worms (Nemertea) Hoplonemertea proboscis worm Nematodes (Nematoda) Mermithidae nematode 1 111 Horsehair worms (Gordioidea) Gordiidae horsehair worm Bryozoans (Bryozoa) Plumatellidae bryozoan, moss animals Annelid worms (Polychaeta) Nereididae polychaete (Oligochaeta) (Microdrili) microdrile worm 138 (Oligochaeta) (Megadrili) earthworm 6212437461 (Hirudinea) Glossiphoniidae leech Crustaceans (Cladocera) water flea (Ostracoda) ostracod, seed shrimp 1 (Eucopepoda) copepod Mysidacea Mysididae opossum shrimp (Isopoda) isopod, sowbug Amphipoda Corophiidae Corophium Amphipoda Crangonyctidae amphipod 72 Amphipoda Hyalellidae amphipod Decopoda Astacidae signal crayfish Decopoda Cambaridae crawfish Decopoda Grapsidae mitten crab Insects Ephemeroptera Ameletidae primitive minnow mayfly Ephemeroptera Siphlonuridae primitive minnow mayfly Ephemeroptera Baetidae small minnow mayfly 24 5 24 2 19 52 10 27 1 Ephemeroptera Heptageniidae flatheaded mayfly 22 Ephemeroptera Ephemerellidae spiny crawler mayfly 6 Ephemeroptera Tricorythidae little stout crawler mayfly 12 2 16 1 52 10 21 1 Ephemeroptera Caenidae squaregill mayfly Ephemeroptera Leptophlebiidae pronggill mayfly Odonata Aeshnidae darner (dragonfly) Odonata Cordulegastridae biddy (dragonfly) Odonata Gomphidae clubtail (dragonfly) Odonata Libellulidae skimmer (dragonfly) Odonata Calopterygidae broadwinged damselfly Odonata Lestidae spreadwinged damselfly Odonata Coenagrionidae narrowwinged damselfly 147 Plecoptera Pteronarcyidae giant stonefly Plecoptera Peltoperlidae roachlike stonefly Plecoptera Nemouridae broadback stonefly Plecoptera Leuctridae rolledwinged stonefly Plecoptera Capniidae slender winter stonefly Plecoptera Perlidae common stonefly Plecoptera Perlodidae perlodid stonefly 11 Plecoptera Chloroperlidae green stonefly Hemiptera Belostomatidae giant water bug Hemiptera Corixidae water boatman Megaloptera Sialidae alderfly 28 Megaloptera Corydalidae fishfly

Gerriadae water strider Hemiptera Veliidae

major group/ order (other) family (other) common name Insects Trichoptera Philopotamidae fingernet caddisfly Trichoptera Psychomyiidae nettube caddisfly Trichoptera Polycentropodidae tubenet caddisfly Trichoptera Hydropsychidae common netspinner 5 2 10 66 15 52 1 Trichoptera Rhyacophilidae freeliving caddisfly Trichoptera Glossosomatidae saddlecase caddisfly 51 32 16 Trichoptera Hydroptilidae micro caddisfly 21 Trichoptera Brachycentridae humpless cased caddisfly Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 55 2 31 15 Trichoptera Limnephilidae northern cased caddisfly 1 Trichoptera Uenoidae (northern cased caddisfly) Trichoptera Sericostomatidae bushtailed cased caddisfly Trichoptera Odontoceridae strongcase caddisfly Trichoptera Leptoceridae longhorned cased caddisfly Lepidoptera Pyralidae aquatic pyralid moth Coleoptera Gyrinidae whirligig beetle Coleoptera Haliplidae crawling water beetle Coleoptera Dytiscidae predaceous diving beetle Coleoptera Hydrophilidae water scavenger beetle Coleoptera Hydraenidae minute moss beetle Coleoptera Psephenidae water penny Coleoptera Dryopidae longtoed water beetle Coleoptera Scirtidae marsh beetle Coleoptera Elmidae riffle beetle 37 4 23 164 84 161 Diptera Blephariceridae netwing midge, button sucker Diptera Tipulidae crane fly 21 3 82 2 Diptera Psychodidae moth fly 27 Diptera Ceratopogonidae biting midge Diptera Simuliidae black fly 11 Diptera Chironomidae chironomid midge 32 8 51 79 21 40 Diptera Chaoboridae phantom midge Diptera Dixidae dixid midge Diptera Stratiomyidae soldier fly Diptera Tabanidae horse or deer fly Diptera Pelecorhynchidae Diptera Dolichopodidae longlegged fly Diptera Empididae dance fly 1 Diptera Syrphidae syrphid, rattailed maggot Diptera Ephydridae shore fly Diptera Sciomyzidae Diptera Muscidae muscid Mites (Acari) (Acari) mite 31252 Mollusks (Gastropoda) Hydrobiidae gilled snails (Gastropoda) Physidae left-handed snail 1 (Gastropoda) Lymnaeidae pond snail (Gastropoda) Planorbidae ramshorn snail 34 12 5 (Bivalvia) Corbiculidae Asian clam (Bivalvia) Sphaeriidae pea or fingernail clam 11 Ancylidae Planorbidae- Ferissa 1 Gastropoda Ancylidae Lepidoptera Nepticulidae Leaf mining moth Hemiptera Veliidae BMI Site Data 2014

Wetted Width ft. Canopy Cover % Covered Site Date/Time Lat Long Water Temp Spec. Cond. DO % DO mg/L pH Turbidity Upper Middle Lower Upper Middle Lower

Dam 7 Sept. 2014, 1:39pm 37.3007 -122.07408 22.97 22.97 710 709 92.2 92.4 7.9 7.91 7.83 7.83 82.2 81.8 15.3 10.5 5.3 70 90 80 Chestnut 7 Sept. 2014, 11:55am 37.3056 -122.07195 18.53 18.48 697 703 82.9 80.1 7.73 7.48 8.01 7.97 33 34.2 22 6.5 6.8 89 90 90 McClellan 6 Sept. 2014, 2:45pm 37.31412 -122.06451 18.73 18.74 745 742 92.4 92.1 8.59 8.57 7.99 7.97 1.7 1.7 14.3 10.9 11.6 80 70 70 La Avenida 7 Sept. 2014, 9:49am 37.41279 -122.06874 19.13 19.13 960 960 91.4 91 8.44 8.38 7.66 7.64 2.1 1.6 13.5 14 9 80 60 50 Charleston 6 Sept. 2014, 12:56pm 21.43 21.46 962 960 92.5 92.5 8.12 8.16 7.82 7.78 4.4 3.4 8 7 9.5 30 20 10

Depth ft. Five repicate sites Velocity ft/sec Five repicate sites Site 12 3 4 5 12345

Dam 10 7.5 9 10 8 7 6.5 6.5 7.5 10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Chestnut 11 12 10 10 10 11 10 13 10 8 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A McClellan 6 7 7 8 5.5 6.5 6 6 7 6.5 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A La Avenida 13.5 14 9 13 10 9 9 9 12 14 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A Charleston 15 19 15 11 12 12.5 10 10.5 10 10 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A

Major Particle Size mm (B axis. Six largest particles in each of five replicate sites Site 12 34 5

Dam 90 39 30 51 36 42 50 58 46 38 36 32 70 50 39 45 42 44 28 43 75 41 48 40 80 40 53 42 49 52 Chestnut 53 55 62 63 95 135 93 91 54 51 34 48 95 87 65 82 125 75 83 75 89 82 50 135 90 50 49 44 60 39 McClellan 52 60 83 52 62 135 68 53 47 42 49 60 33 34 70 53 81 88 32 40 54 67 82 59 55 38 34 43 46 79 La Avenida 46 39 22 23 16 18 57 35 30 45 31 39 71 55 30 49 47 82 52 36 47 41 42 39 85 47 35 28 27 56 Charleston 161 23 26 21 15 12 34 42 29 86 35 60 39 36 62 40 35 37 26 53 21 36 64 78 24 25 27 22 49 68 BMI Sampling Fall of 2014 DA1 DA2 DA3 DA4 DA5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra 00000 Fla tw orms 00000 Tricladida Planariidae flatworm 00000 Proboscis worms 00000 (Nemertea) Hoplonemertea proboscis worm 00000 Nematodes 00000 (Nematoda) Mermithidae nematode 3 3 01123 3 0 Horsehair worms 00000 (Gordioidea) Gordiidae horsehair worm 00000 Bryozoans 00000 (Bryozoa) Plumatellidae bryozoan, moss animals 00000 Annelid worms 00000 (Polychaeta) Nereididae polychaete 00000 (Oligochaeta) (Microdrili) microdrile worm 58 4 62 22 128 150 10 4 14 109 7 116 24 5 29 (Oligochaeta) (Megadrili) earthworm 0 0 01111 (Hirudinea) Glossiphoniidae leech 00000 Crustaceans 00000 (Cladocera) water flea 00000 (Ostracoda) ostracod, seed shrimp 11110220 (Eucopepoda) copepod 00000 Mysidacea Mysididae opossum shrimp 00000 (Isopoda) isopod, sowbug 00000 Amphipoda Corophiidae Corophium 00000 Amphipoda Crangonyctidae amphipod 22000112 Amphipoda Gammaridae amphipod 00000 Amphipoda Hyalellidae amphipod 2 22137 71 1112 Decopoda Astacidae signal crayfish 00000 Decopoda Cambaridae crawfish 00000 Decopoda Grapsidae mitten crab 00000 Insects 00000 Ephemeroptera Ameletidae primitive minnow mayfly 00000 Ephemeroptera Siphlonuridae primitive minnow mayfly 00000 Ephemeroptera Baetidae small minnow mayfly 6611111155 Ephemeroptera Heptageniidae flatheaded mayfly 00000 Ephemeroptera Ephemerellidae spiny crawler mayfly 00000 Ephemeroptera Tricorythidae little stout crawler mayfly 000110 Ephemeroptera Caenidae squaregill mayfly 00000 Ephemeroptera Leptophlebiidae pronggill mayfly 00000 Odonata Aeshnidae darner (dragonfly) 00000 Odonata Cordulegastridae biddy (dragonfly) 00000 Odonata Gomphidae clubtail (dragonfly) 00000 Odonata Libellulidae skimmer (dragonfly) 00000 Odonata Calopterygidae broadwinged damselfly 00000 Odonata Lestidae spreadwinged damselfly 00000 Odonata Coenagrionidae narrowwinged damselfly 9 9 14 1 15 18 1 19 10 1 11 8 8 Plecoptera Pteronarcyidae giant stonefly 00000 Plecoptera Peltoperlidae roachlike stonefly 00000 Plecoptera Nemouridae broadback stonefly 011000 Plecoptera Leuctridae rolledwinged stonefly 00000 Plecoptera Capniidae slender winter stonefly 00000 Plecoptera Perlidae common stonefly 00000 Plecoptera Perlodidae perlodid stonefly 00000 Plecoptera Chloroperlidae green stonefly 00000 Hemiptera Belostomatidae giant water bug 00000 Hemiptera Corixidae water boatman 00000 Megaloptera Sialidae alderfly 02205566 Megaloptera Corydalidae fishfly 00000 00000 Gerriadae water strider 00000 Hemiptera Veliidae 00000 00000 00000 major group/ order (other) family (other) common name 00000 Insects 00000 Trichoptera Philopotamidae fingernet caddisfly 00000 Trichoptera Psychomyiidae nettube caddisfly 00000 Trichoptera Polycentropodidae tubenet caddisfly 00000 Trichoptera Hydropsychidae common netspinner 28129153184714810 1027229 Trichoptera Rhyacophilidae freeliving caddisfly 00000 Trichoptera Glossosomatidae saddlecase caddisfly 00000 Trichoptera Hydroptilidae micro caddisfly 66 0223311 Trichoptera Brachycentridae humpless cased caddisfly 00000 Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 00000 Trichoptera Limnephilidae northern cased caddisfly 00000 Trichoptera Uenoidae (northern cased caddisfly) 00000 Trichoptera Sericostomatidae bushtailed cased caddisfly 00000 Trichoptera Odontoceridae strongcase caddisfly 00000 Trichoptera Leptoceridae longhorned cased caddisfly 00000 Lepidoptera Pyralidae aquatic pyralid moth 00000 Coleoptera Gyrinidae whirligig beetle 00000 Coleoptera Haliplidae crawling water beetle 00000 Coleoptera Dytiscidae predaceous diving beetle 00000 Coleoptera Hydrophilidae water scavenger beetle 00000 Coleoptera Hydraenidae minute moss beetle 00000 Coleoptera Psephenidae water penny 00000 Coleoptera Dryopidae longtoed water beetle 00000 Coleoptera Scirtidae marsh beetle 00000 Coleoptera Elmidae riffle beetle 00000 Diptera Blephariceridae netwing midge, button sucker 00000 Diptera Tipulidae crane fly 4433331166 Diptera Psychodidae moth fly 00000 Diptera Ceratopogonidae biting midge 00022411 Diptera Simuliidae black fly 8 81238194 15516 Diptera Chironomidae chironomid midge 828903752894912611775182742498 Diptera Chaoboridae phantom midge 00000 Diptera Dixidae dixid midge 00000 Diptera Stratiomyidae soldier fly 00000 Diptera Tabanidae horse or deer fly 00000 Diptera Pelecorhynchidae 00000 Diptera Dolichopodidae longlegged fly 00000 Diptera Empididae dance fly 2 2 333 34153 3 Diptera Syrphidae syrphid, rattailed maggot 00000 Diptera Ephydridae shore fly 00000 Diptera Sciomyzidae 00000 Diptera Muscidae muscid 00000 Mites 00000 (Acari) (Acari) mite 011000 Mollusks 00000 (Gastropoda) Hydrobiidae gilled snails 00000 (Gastropoda) Physidae left-handed snail 00000 (Gastropoda) Lymnaeidae pond snail 00000 (Gastropoda) Planorbidae ramshorn snail 000110 (Bivalvia) Corbiculidae Asian clam 00000 (Bivalvia) Sphaeriidae pea or fingernail clam 2211221212 0 Ancylidae 00000 Gastropoda hudrobiidae New Zealand Mudsnail 00000 Gastropoda Ancylidae 00000 Lepidoptera Nepticulidae Leaf mining moth 00000 Hemiptera Veliidae 00000 BMI Sampling Fall of 2014 CH1 CH2 CH3 CH4 CH5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra 00000 Fla tw orms 00000 Tricladida Planariidae flatworm 00000 Proboscis worms 00000 (Nemertea) Hoplonemertea proboscis worm 110 01111 Nematodes 00000 (Nematoda) Mermithidae nematode 00000 Horsehair worms 00000 (Gordioidea) Gordiidae horsehair worm 00000 Bryozoans 00000 (Bryozoa) Plumatellidae bryozoan, moss animals 00000 Annelid worms 00000 (Polychaeta) Nereididae polychaete 00000 (Oligochaeta) (Microdrili) microdrile worm 1123 32 21 16 6 (Oligochaeta) (Megadrili) earthworm 0 111 1 01 1 (Hirudinea) Glossiphoniidae leech 00000 Crustaceans 00000 (Cladocera) water flea 00000 (Ostracoda) ostracod, seed shrimp 00000 (Eucopepoda) copepod 00000 Mysidacea Mysididae opossum shrimp 00000 (Isopoda) isopod, sowbug 000110 Amphipoda Corophiidae Corophium 00000 Amphipoda Crangonyctidae amphipod 14 141541915 1517 1711 11 Amphipoda Gammaridae amphipod 00000 Amphipoda Hyalellidae amphipod 00000 Decopoda Astacidae signal crayfish 00000 Decopoda Cambaridae crawfish 00000 Decopoda Grapsidae mitten crab 00000 Insects 00000 Ephemeroptera Ameletidae primitive minnow mayfly 00000 Ephemeroptera Siphlonuridae primitive minnow mayfly 00000 Ephemeroptera Baetidae small minnow mayfly 23225504546947344 4499 99 Ephemeroptera Heptageniidae flatheaded mayfly 00000 Ephemeroptera Ephemerellidae spiny crawler mayfly 00000 Ephemeroptera Tricorythidae little stout crawler mayfly 110000 Ephemeroptera Caenidae squaregill mayfly 00000 Ephemeroptera Leptophlebiidae pronggill mayfly 00000 Odonata Aeshnidae darner (dragonfly) 00000 Odonata Cordulegastridae biddy (dragonfly) 00000 Odonata Gomphidae clubtail (dragonfly) 00000 Odonata Libellulidae skimmer (dragonfly) 00000 Odonata Calopterygidae broadwinged damselfly 00000 Odonata Lestidae spreadwinged damselfly 00000 Odonata Coenagrionidae narrowwinged damselfly 819213101013131111 Plecoptera Pteronarcyidae giant stonefly 00000 Plecoptera Peltoperlidae roachlike stonefly 00000 Plecoptera Nemouridae broadback stonefly 022000 Plecoptera Leuctridae rolledwinged stonefly 00000 Plecoptera Capniidae slender winter stonefly 00000 Plecoptera Perlidae common stonefly 00000 Plecoptera Perlodidae perlodid stonefly 000011 Plecoptera Chloroperlidae green stonefly 00000 Hemiptera Belostomatidae giant water bug 00000 Hemiptera Corixidae water boatman 00000 Megaloptera Sialidae alderfly 11110220 Megaloptera Corydalidae fishfly 0110022 00000 Gerriadae water strider 00000 Hemiptera Veliidae 00000 00000 00000 major group/ order (other) family (other) common name 00000 Insects 00000 Trichoptera Philopotamidae fingernet caddisfly 00000 Trichoptera Psychomyiidae nettube caddisfly 00000 Trichoptera Polycentropodidae tubenet caddisfly 00000 Trichoptera Hydropsychidae common netspinner 01103366 Trichoptera Rhyacophilidae freeliving caddisfly 00000 Trichoptera Glossosomatidae saddlecase caddisfly 00000 Trichoptera Hydroptilidae micro caddisfly 114594 45 51 1 Trichoptera Brachycentridae humpless cased caddisfly 00000 Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 02 2 01 1 0 Trichoptera Limnephilidae northern cased caddisfly 00000 Trichoptera Uenoidae (northern cased caddisfly) 00000 Trichoptera Sericostomatidae bushtailed cased caddisfly 00000 Trichoptera Odontoceridae strongcase caddisfly 00000 Trichoptera Leptoceridae longhorned cased caddisfly 00000 Lepidoptera Pyralidae aquatic pyralid moth 00000 Coleoptera Gyrinidae whirligig beetle 00000 Coleoptera Haliplidae crawling water beetle 00000 Coleoptera Dytiscidae predaceous diving beetle 00000 Coleoptera Hydrophilidae water scavenger beetle 00000 Coleoptera Hydraenidae minute moss beetle 00000 Coleoptera Psephenidae water penny 00000 Coleoptera Dryopidae longtoed water beetle 00000 Coleoptera Scirtidae marsh beetle 00000 Coleoptera Elmidae riffle beetle 00000 Diptera Blephariceridae netwing midge, button sucker 00000 Diptera Tipulidae crane fly 03142 22 24 4 Diptera Psychodidae moth fly 00000 Diptera Ceratopogonidae biting midge 002200 Diptera Simuliidae black fly 2 2 99 17 116 37 2 39 0 85 85 Diptera Chironomidae chironomid midge 76 10 86 23 3 26 65 9 74 72 72 47 47 Diptera Chaoboridae phantom midge 00000 Diptera Dixidae dixid midge 00000 Diptera Stratiomyidae soldier fly 001100 Diptera Tabanidae horse or deer fly 00000 Diptera Pelecorhynchidae 00000 Diptera Dolichopodidae longlegged fly 00000 Diptera Empididae dance fly 00000 Diptera Syrphidae syrphid, rattailed maggot 00000 Diptera Ephydridae shore fly 00000 Diptera Sciomyzidae 00000 Diptera Muscidae muscid 00000 Mites 00000 (Acari) (Acari) mite 00000 Mollusks 00000 (Gastropoda) Hydrobiidae gilled snails 00000 (Gastropoda) Physidae left-handed snail 0 12 12 0 2 2 14 14 (Gastropoda) Lymnaeidae pond snail 00000 (Gastropoda) Planorbidae ramshorn snail 00000 (Bivalvia) Corbiculidae Asian clam 00000 (Bivalvia) Sphaeriidae pea or fingernail clam 000011 Ancylidae 00000 Gastropoda hudrobiidae New Zealand Mudsnail 00000 Gastropoda Ancylidae 00000 Lepidoptera Nepticulidae Leaf mining moth 00000 Hemiptera Veliidae 00000 BMI Sampling Fall of 2014 MC1 MC2 MC3 MC4 MC5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra 00000 Fla tw orms 00000 Tricladida Planariidae flatworm 404443464927 272283036 36 Proboscis worms 00000 (Nemertea) Hoplonemertea proboscis worm 000011 Nematodes 00000 (Nematoda) Mermithidae nematode 0112000 Horsehair worms 00000 (Gordioidea) Gordiidae horsehair worm 00000 Bryozoans 00000 (Bryozoa) Plumatellidae bryozoan, moss animals 00000 Annelid worms 00000 (Polychaeta) Nereididae polychaete 00000 (Oligochaeta) (Microdrili) microdrile worm 1 100220 (Oligochaeta) (Megadrili) earthworm 15 15 18 18 10 10 30 30 19 1 20 (Hirudinea) Glossiphoniidae leech 00000 Crustaceans 00000 (Cladocera) water flea 00000 (Ostracoda) ostracod, seed shrimp 00000 (Eucopepoda) copepod 00000 Mysidacea Mysididae opossum shrimp 00000 (Isopoda) isopod, sowbug 00000 Amphipoda Corophiidae Corophium 00000 Amphipoda Crangonyctidae amphipod 0 2 2 0112 0 Amphipoda Gammaridae amphipod 00000 Amphipoda Hyalellidae amphipod 00000 Decopoda Astacidae signal crayfish 00000 Decopoda Cambaridae crawfish 00000 Decopoda Grapsidae mitten crab 00000 Insects 00000 Ephemeroptera Ameletidae primitive minnow mayfly 00000 Ephemeroptera Siphlonuridae primitive minnow mayfly 00000 Ephemeroptera Baetidae small minnow mayfly 32 32 9110 14 142563123124 Ephemeroptera Heptageniidae flatheaded mayfly 00000 Ephemeroptera Ephemerellidae spiny crawler mayfly 00000 Ephemeroptera Tricorythidae little stout crawler mayfly 30 30 82 16 98 9 922462264 Ephemeroptera Caenidae squaregill mayfly 00000 Ephemeroptera Leptophlebiidae pronggill mayfly 00000 Odonata Aeshnidae darner (dragonfly) 00000 Odonata Cordulegastridae biddy (dragonfly) 00000 Odonata Gomphidae clubtail (dragonfly) 00000 Odonata Libellulidae skimmer (dragonfly) 00000 Odonata Calopterygidae broadwinged damselfly 00000 Odonata Lestidae spreadwinged damselfly 00000 Odonata Coenagrionidae narrowwinged damselfly 000022 Plecoptera Pteronarcyidae giant stonefly 00000 Plecoptera Peltoperlidae roachlike stonefly 00000 Plecoptera Nemouridae broadback stonefly 00000 Plecoptera Leuctridae rolledwinged stonefly 00000 Plecoptera Capniidae slender winter stonefly 00000 Plecoptera Perlidae common stonefly 00000 Plecoptera Perlodidae perlodid stonefly 00000 Plecoptera Chloroperlidae green stonefly 00000 Hemiptera Belostomatidae giant water bug 00000 Hemiptera Corixidae water boatman 00000 Megaloptera Sialidae alderfly 0 2 2 3 33 3 0 Megaloptera Corydalidae fishfly 00000 00000 Gerriadae water strider 00000 Hemiptera Veliidae 00000 00000 00000 major group/ order (other) family (other) common name 00000 Insects 00000 Trichoptera Philopotamidae fingernet caddisfly 00000 Trichoptera Psychomyiidae nettube caddisfly 00000 Trichoptera Polycentropodidae tubenet caddisfly 00000 Trichoptera Hydropsychidae common netspinner 042145669 6942115348250 Trichoptera Rhyacophilidae freeliving caddisfly 00000 Trichoptera Glossosomatidae saddlecase caddisfly 0003344 Trichoptera Hydroptilidae micro caddisfly 3140000 Trichoptera Brachycentridae humpless cased caddisfly 00000 Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 1631914314538 38 032 32 Trichoptera Limnephilidae northern cased caddisfly 00000 Trichoptera Uenoidae (northern cased caddisfly) 00000 Trichoptera Sericostomatidae bushtailed cased caddisfly 00000 Trichoptera Odontoceridae strongcase caddisfly 00000 Trichoptera Leptoceridae longhorned cased caddisfly 00000 Lepidoptera Pyralidae aquatic pyralid moth 00000 Coleoptera Gyrinidae whirligig beetle 00000 Coleoptera Haliplidae crawling water beetle 00000 Coleoptera Dytiscidae predaceous diving beetle 00000 Coleoptera Hydrophilidae water scavenger beetle 00000 Coleoptera Hydraenidae minute moss beetle 00000 Coleoptera Psephenidae water penny 00000 Coleoptera Dryopidae longtoed water beetle 00000 Coleoptera Scirtidae marsh beetle 00000 Coleoptera Elmidae riffle beetle 99 11 110 112 45 157 68 68 120 120 115 4 119 Diptera Blephariceridae netwing midge, button sucker 00000 Diptera Tipulidae crane fly 2 20000 Diptera Psychodidae moth fly 00000 Diptera Ceratopogonidae biting midge 5160000 Diptera Simuliidae black fly 5 5 1 10022 Diptera Chironomidae chironomid midge 41 7 48 24 14 38 45 457132058462 Diptera Chaoboridae phantom midge 00000 Diptera Dixidae dixid midge 00000 Diptera Stratiomyidae soldier fly 00000 Diptera Tabanidae horse or deer fly 00000 Diptera Pelecorhynchidae 000110 Diptera Dolichopodidae longlegged fly 00000 Diptera Empididae dance fly 0 1 1000 Diptera Syrphidae syrphid, rattailed maggot 00000 Diptera Ephydridae shore fly 00000 Diptera Sciomyzidae 00000 Diptera Muscidae muscid 00000 Mites 00000 (Acari) (Acari) mite 415 3 3 1 1022 Mollusks 00000 (Gastropoda) Hydrobiidae gilled snails 00000 (Gastropoda) Physidae left-handed snail 0 0 01144 (Gastropoda) Lymnaeidae pond snail 0 123 01122 (Gastropoda) Planorbidae ramshorn snail 1 1 2130033 (Bivalvia) Corbiculidae Asian clam 21 21 10 3 13 21 214124328 28 (Bivalvia) Sphaeriidae pea or fingernail clam 1 1 1120110 Ancylidae 00000 Gastropoda hudrobiidae New Zealand Mudsnail 00111100 Gastropoda Ancylidae 00000 Lepidoptera Nepticulidae Leaf mining moth 00000 Hemiptera Veliidae 00000 BMI Sampling Fall of 2014 LA1 LA2 LA3 LA4 LA5 major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra 00000 Fla tw orms 00000 Tricladida Planariidae flatworm 3 31568128327274 4 Proboscis worms 00000 (Nemertea) Hoplonemertea proboscis worm 00000 Nematodes 00000 (Nematoda) Mermithidae nematode 000110 Horsehair worms 00000 (Gordioidea) Gordiidae horsehair worm 00000 Bryozoans 00000 (Bryozoa) Plumatellidae bryozoan, moss animals 00000 Annelid worms 00000 (Polychaeta) Nereididae polychaete 00000 (Oligochaeta) (Microdrili) microdrile worm 222240 066 (Oligochaeta) (Megadrili) earthworm 10 10 18 18 77 77 55 55 51 1 52 (Hirudinea) Glossiphoniidae leech 00000 Crustaceans 00000 (Cladocera) water flea 00000 (Ostracoda) ostracod, seed shrimp 00000 (Eucopepoda) copepod 00000 Mysidacea Mysididae opossum shrimp 00000 (Isopoda) isopod, sowbug 011000 Amphipoda Corophiidae Corophium 00000 Amphipoda Crangonyctidae amphipod 11 11 1 1 1 1 2 0 1 1 Amphipoda Gammaridae amphipod 00000 Amphipoda Hyalellidae amphipod 0122141 1 0 2 2 Decopoda Astacidae signal crayfish 00000 Decopoda Cambaridae crawfish 00000 Decopoda Grapsidae mitten crab 00000 Insects 00000 Ephemeroptera Ameletidae primitive minnow mayfly 00000 Ephemeroptera Siphlonuridae primitive minnow mayfly 00000 Ephemeroptera Baetidae small minnow mayfly 67 67 205 15 220 332 13 345 74 74 37 15 52 Ephemeroptera Heptageniidae flatheaded mayfly 00000 Ephemeroptera Ephemerellidae spiny crawler mayfly 00000 Ephemeroptera Tricorythidae little stout crawler mayfly 001104913 Ephemeroptera Caenidae squaregill mayfly 00000 Ephemeroptera Leptophlebiidae pronggill mayfly 00000 Odonata Aeshnidae darner (dragonfly) 00000 Odonata Cordulegastridae biddy (dragonfly) 00000 Odonata Gomphidae clubtail (dragonfly) 00000 Odonata Libellulidae skimmer (dragonfly) 00000 Odonata Calopterygidae broadwinged damselfly 00000 Odonata Lestidae spreadwinged damselfly 00000 Odonata Coenagrionidae narrowwinged damselfly 00000 Plecoptera Pteronarcyidae giant stonefly 00000 Plecoptera Peltoperlidae roachlike stonefly 00000 Plecoptera Nemouridae broadback stonefly 00000 Plecoptera Leuctridae rolledwinged stonefly 00000 Plecoptera Capniidae slender winter stonefly 00000 Plecoptera Perlidae common stonefly 00000 Plecoptera Perlodidae perlodid stonefly 00000 Plecoptera Chloroperlidae green stonefly 00000 Hemiptera Belostomatidae giant water bug 00000 Hemiptera Corixidae water boatman 000011 Megaloptera Sialidae alderfly 00000 Megaloptera Corydalidae fishfly 00000 00000 Gerriadae water strider 00000 Hemiptera Veliidae 00000 00000 00000 major group/ order (other) family (other) common name 00000 Insects 00000 Trichoptera Philopotamidae fingernet caddisfly 00000 Trichoptera Psychomyiidae nettube caddisfly 44000617 Trichoptera Polycentropodidae tubenet caddisfly 00000 Trichoptera Hydropsychidae common netspinner 01 16 67 7 0 Trichoptera Rhyacophilidae freeliving caddisfly 00000 Trichoptera Glossosomatidae saddlecase caddisfly 00000 Trichoptera Hydroptilidae micro caddisfly 11 01631911 0 Trichoptera Brachycentridae humpless cased caddisfly Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 00000 Trichoptera Limnephilidae northern cased caddisfly 00000 Trichoptera Uenoidae (northern cased caddisfly) 00000 Trichoptera Sericostomatidae bushtailed cased caddisfly 00000 Trichoptera Odontoceridae strongcase caddisfly 00000 Trichoptera Leptoceridae longhorned cased caddisfly 00000 Lepidoptera Pyralidae aquatic pyralid moth 00000 Coleoptera Gyrinidae whirligig beetle 00000 Coleoptera Haliplidae crawling water beetle 00000 Coleoptera Dytiscidae predaceous diving beetle 00000 Coleoptera Hydrophilidae water scavenger beetle 00000 Coleoptera Hydraenidae minute moss beetle 00000 Coleoptera Psephenidae water penny 00000 Coleoptera Dryopidae longtoed water beetle 00000 Coleoptera Scirtidae marsh beetle 00000 Coleoptera Elmidae riffle beetle 00000 Diptera Blephariceridae netwing midge, button sucker 4 4 2 2 14 14 38 38 11 1 12 Diptera Tipulidae crane fly 00000 Diptera Psychodidae moth fly 000110 Diptera Ceratopogonidae biting midge 00000 Diptera Simuliidae black fly 00000 Diptera Chironomidae chironomid midge 01 1963997 71 1 Diptera Chaoboridae phantom midge 3 3 3 3 11 11 1 1 1 3 4 Diptera Dixidae dixid midge 00000 Diptera Stratiomyidae soldier fly 00000 Diptera Tabanidae horse or deer fly 00000 Diptera Pelecorhynchidae 00000 Diptera Dolichopodidae longlegged fly 00000 Diptera Empididae dance fly 00000 Diptera Syrphidae syrphid, rattailed maggot 00000 Diptera Ephydridae shore fly 00000 Diptera Sciomyzidae 00000 Diptera Muscidae muscid 00000 Mites 00000 (Acari) (Acari) mite 00000 Mollusks 002200 (Gastropoda) Hydrobiidae gilled snails 00000 (Gastropoda) Physidae left-handed snail 00000 (Gastropoda) Lymnaeidae pond snail 00000 (Gastropoda) Planorbidae ramshorn snail 11 011 0 0 (Bivalvia) Corbiculidae Asian clam 01 12 2 0 0 (Bivalvia) Sphaeriidae pea or fingernail clam 00000 Ancylidae 001100 Gastropoda hudrobiidae New Zealand Mudsnail 00000 Gastropoda Ancylidae 00000 Lepidoptera Nepticulidae Leaf mining moth 00000 Hemiptera Veliidae 00000 BMI Sampling Fall of 2014 CR1 CR2 CR3 CR4 CR5 Charleston major group/ order (other) family (other) common name count QC count total count QC count total count QC count total count QC count total count QC count total Cnidarians (Hydrozoa) (Hydrozoa) Hydra 00NA00 Fla tw orms 00NA00 Tricladida Planariidae flatworm 00NA00 Proboscis worms 00NA00 (Nemertea) Hoplonemertea proboscis worm 00NA00 Nematodes 00NA00 (Nematoda) Mermithidae nematode 00NA011 Horsehair worms 00NA00 (Gordioidea) Gordiidae horsehair worm 00NA00 Bryozoans 00NA00 (Bryozoa) Plumatellidae bryozoan, moss animals 00NA00 Annelid worms 00NA00 (Polychaeta) Nereididae polychaete 1 1 0 NA1 1415 (Oligochaeta) (Microdrili) microdrile worm 3311 NA220 (Oligochaeta) (Megadrili) earthworm 00NA110 (Hirudinea) Glossiphoniidae leech 00NA00 Crustaceans 00NA00 (Cladocera) water flea 00NA00 (Ostracoda) ostracod, seed shrimp 00NA00 (Eucopepoda) copepod 00NA00 Mysidacea Mysididae opossum shrimp 00NA00 (Isopoda) isopod, sowbug 2122333 33 NA68117967480 Amphipoda Corophiidae Corophium 161 7 168 214 10 224 NA 319 120 439 401 20 421 Amphipoda Crangonyctidae amphipod 00NA00 Amphipoda Gammaridae amphipod 132 7 139 261 261 NA 302 74 376 388 6 394 Amphipoda Hyalellidae amphipod 00NA00 Decopoda Astacidae signal crayfish 00NA00 Decopoda Cambaridae crawfish 00NA00 Decopoda Grapsidae mitten crab 00NA00 Insects 00NA00 Ephemeroptera Ameletidae primitive minnow mayfly 00NA00 Ephemeroptera Siphlonuridae primitive minnow mayfly 00NA00 Ephemeroptera Baetidae small minnow mayfly 00NA00 Ephemeroptera Heptageniidae flatheaded mayfly 00NA00 Ephemeroptera Ephemerellidae spiny crawler mayfly 00NA00 Ephemeroptera Tricorythidae little stout crawler mayfly 00NA00 Ephemeroptera Caenidae squaregill mayfly 00NA00 Ephemeroptera Leptophlebiidae pronggill mayfly 00NA00 Odonata Aeshnidae darner (dragonfly) 00NA00 Odonata Cordulegastridae biddy (dragonfly) 00NA00 Odonata Gomphidae clubtail (dragonfly) 00NA00 Odonata Libellulidae skimmer (dragonfly) 00NA00 Odonata Calopterygidae broadwinged damselfly 00NA00 Odonata Lestidae spreadwinged damselfly 00NA00 Odonata Coenagrionidae narrowwinged damselfly 011NA 0 0 Plecoptera Pteronarcyidae giant stonefly 00NA00 Plecoptera Peltoperlidae roachlike stonefly 00NA00 Plecoptera Nemouridae broadback stonefly 00NA00 Plecoptera Leuctridae rolledwinged stonefly 00NA00 Plecoptera Capniidae slender winter stonefly 00NA00 Plecoptera Perlidae common stonefly 00NA00 Plecoptera Perlodidae perlodid stonefly 00NA00 Plecoptera Chloroperlidae green stonefly 00NA00 Hemiptera Belostomatidae giant water bug 00NA00 Hemiptera Corixidae water boatman 00NA00 Megaloptera Sialidae alderfly 011NA 0 0 Megaloptera Corydalidae fishfly 00NA00 00NA00 Gerriadae water strider 00NA00 Hemiptera Veliidae 00NA00 00NA00 00NA00 major group/ order (other) family (other) common name 00NA00 Insects 00NA00 Trichoptera Philopotamidae fingernet caddisfly 00NA00 Trichoptera Psychomyiidae nettube caddisfly 1111 NA61711 Trichoptera Polycentropodidae tubenet caddisfly 00NA00 Trichoptera Hydropsychidae common netspinner 00NA00 Trichoptera Rhyacophilidae freeliving caddisfly 00NA00 Trichoptera Glossosomatidae saddlecase caddisfly 00NA00 Trichoptera Hydroptilidae micro caddisfly 00NA110 Trichoptera Brachycentridae humpless cased caddisfly 00NA00 Trichoptera Lepidostomatidae lepidostomatid cased caddisfly 00NA00 Trichoptera Limnephilidae northern cased caddisfly 00NA00 Trichoptera Uenoidae (northern cased caddisfly) 00NA00 Trichoptera Sericostomatidae bushtailed cased caddisfly 00NA00 Trichoptera Odontoceridae strongcase caddisfly 00NA00 Trichoptera Leptoceridae longhorned cased caddisfly 00NA00 Lepidoptera Pyralidae aquatic pyralid moth 00NA00 Coleoptera Gyrinidae whirligig beetle 00NA00 Coleoptera Haliplidae crawling water beetle 00NA00 Coleoptera Dytiscidae predaceous diving beetle 00NA00 Coleoptera Hydrophilidae water scavenger beetle 00NA00 Coleoptera Hydraenidae minute moss beetle 00NA00 Coleoptera Psephenidae water penny 00NA00 Coleoptera Dryopidae longtoed water beetle 00NA00 Coleoptera Scirtidae marsh beetle 00NA00 Coleoptera Elmidae riffle beetle 5 51 1 NA72919120 Diptera Blephariceridae netwing midge, button sucker 00NA00 Diptera Tipulidae crane fly 00NA00 Diptera Psychodidae moth fly 00NA00 Diptera Ceratopogonidae biting midge 00NA110 Diptera Simuliidae black fly 00NA00 Diptera Chironomidae chironomid midge 4444 NA53866 Diptera Chaoboridae phantom midge 00NA00 Diptera Dixidae dixid midge 00NA00 Diptera Stratiomyidae soldier fly 00NA00 Diptera Tabanidae horse or deer fly 00NA00 Diptera Pelecorhynchidae 00NA00 Diptera Dolichopodidae longlegged fly 00NA00 Diptera Empididae dance fly 00NA00 Diptera Syrphidae syrphid, rattailed maggot 00NA00 Diptera Ephydridae shore fly 00NA00 Diptera Sciomyzidae 00NA00 Diptera Muscidae muscid 00NA00 Mites 00NA00 (Acari) (Acari) mite 00NA00 Mollusks 00NA00 (Gastropoda) Hydrobiidae gilled snails 00NA00 (Gastropoda) Physidae left-handed snail 00NA00 (Gastropoda) Lymnaeidae pond snail 00NA00 (Gastropoda) Planorbidae ramshorn snail 00NA00 (Bivalvia) Corbiculidae Asian clam 4 4 3 3 NA 13 13 3 3 (Bivalvia) Sphaeriidae pea or fingernail clam 00NA00 Ancylidae 00NA00 Gastropoda hudrobiidae New Zealand Mudsnail 00NA00 Gastropoda Ancylidae 00NA00 Lepidoptera Nepticulidae Leaf mining moth 00NA00 Hemiptera Veliidae 00NA00 H S W E P

Outline of Field Protocol for MVHS Bug Sampling 2015 Modified Targeted Riffle Sampling BEFORE SAMPLING

 Sketch the site to show flow, riffles/pools, large rocks, woody debris, and bank characteristics and identifying features. As sample sites are selected, mark these on the sketch with Xs.  Record water chemistry – temperature, dissolved oxygen, specific conductance, pH, and turbidity.  Eyeball where each of the eight samples will be collected. Sampling should start from the downstream end and progress upstream. Sample location is supposed to be random within the riffle, but water levels are so low this year, we will need to do it where it makes sense.  Have a practice run through of sampling techniques eon th bank to give the students an idea of what will be happening in the creek when we are actually collecting samples. The biggest concern here is that it needs to be emphasized that everyone needs to stay behind the designated leader in the creek so that we do not trample the areas where we will be collecting. SAMPLING

 For each sample, place the D‐net firmly on the bottom of the creek perpendicular to water flow.  Place the square foot template in front of the net.  Measure the depth at two more or less random places within the square foot area. Record.  Pick out thex si biggest particles (rocks), rinse them of bugs into the net, and then place them in a wash pan with a bit of water and take them to the creek bank.  Feel around the sample area for caddis fly cases, clams etc and place them into the net.  With gloved hands, agitate the creek bottom within the template to a depth of about 10 cm for 60 seconds. Bugs and debris will flow into the net.  When the water clears, carefully lift the net, and encourage the contents to settle to the bottom.  Go on to the next riffle area and repeat the process. Do this for eight square foot samples. If the net gets too full before all eight samples are collected, empty the net into a wash tub and continue sampling.  When all eight samples are collected, deposit them in a dish pan with a bit of water. Pick stubborn bugs from the net and put them into the dish pan as well. Elutriate the sample to remove excess rocks and sediment.  Process the large sediment particles previously set aside from each sample area. Add water to pan, carefully clean all bugs off the rocks and then place the rocks in a second dish pan. Pour buggy water from first dish pan in with main bug sample.  Place the bug sample into the small net, flush it with a bit of ethanol, and then turn the net inside out, placing the bugs in a large labeled plastic jar. Add enough ethanol to cover.

AFTER SAMPLING

 Measure and record the B‐axis of each of the large particles (rocks) set aside in the second dish pan. B‐AXIS  After collecting eight samples, measure wetted channel width and canopy cover at the upper, middle, and lower areas of the sampling site. H S W E P Before Bug Week Worksheet—Page 1

1. Why are we having Bug Week? (choose one or more answers)

A. The kinds of bugs we find in our samples can tell us how healthy or unhealthy the creek is. This is because we know some bugs can only live in clean water, while other bugs are not fussyd an can live in polluted or dirty water. If we only find bugs which are not fussy, there may be a polluon problem. B. Students will learn how to carry out a real‐world ecological study which will help the water district, the state, and local cies be er manage the creek. C. Bugs are awesome. D. All of the above

2. How can we possibly learn to idenfy bugs since they all look alike? (choose one or more answers) A. If we learn what the lile bug bits are called and how to use an idenficaon key, we can just follow the direcons in the key to figure out which bugs are in our samples. The bugs do not really all look alike. B. If we close our eyes and think hard enough, the name of the bug will come to us. C. It does not maer anyway and it is okay to make names up. Kevin is a good name.

3. Okay, let’s start by learning some bug bits. Below on the le is a drawing of a bug. On the right are the names and descripons of some bug bits. Using the descripons, label the bug bits in the drawing. We have started you off by labeling the thorax for you.

Bug Bits Head—secon of body in front of the thorax. Contains the mouth parts and major sense or‐ gans. Thorax—secon of body between the head and the abdomen. Segmented legs and wings/ Thorax wingpads (if present) are always aached to the thorax. Abdomen—rear end secon of body behind the thorax. Abdominal segment—part or division of the abdomen. Antennae—slender, movable sensory organs at head of bug. Gills—breathing structures (quite oen leaf‐ or feather‐shaped and along the side of the abdomen). Segmented leg—true leg; jointed (hinged) leg capable of bending. Tail(s)—hair‐like, bristly, or paddle‐shaped structure(s) extending from end of abdomen. Wing pads—underdeveloped wings, oen in “W” shape, on thorax.

Acterra, Acon for a Healthy Planet, 3921 East Bayshore Road, Palo Alto 94303 H S W E P

Before Bug Week Worksheet—Page 2

Now that you know some bug bits, let’s pracce using a key to idenfy a bug. To the right is a picture of our bug (his name is not Kevin).

 When you use an idenficaon key, you will start at the beginning.

 Each step in the key will generally give you two choices of bug descripons.

 Circle the bug descripon that is correct for your bug (we circled the first correct descripon for you). Then connue below to the next step. Note: most of the bugs we will be idenfying are in their larval or nymph  You will have another choice to make; again circle the bug descripon stage, which is to say they are “baby” that is correct for your bug. bugs. Their adult form will live on land  Keep going down through the steps, circling the correct descripons, and usually fly. unl you find your bug. Then circle the correct picture and BUG NAME (noce how each bug name is bold, underlined, and in capital leers).

Start here

So‐body enclosed within a hard So‐body not enclosed within a calcium shell (clam or snail) hard calcium shell.

Body enclosed Body enclosed in No segmented legs Segmented legs in single shell. two hinged shells. capable of bending. capable of bending.

PHYSIDAE CORBICULIDAE

End of abdomen End of abdomen has 2 or 3 long, has short brush of thin, hair‐like tails. hairs. Thorax has 3 hardened plates.

HYDROPSYCHIDAE Has some Has no obvious appendages appendages 3 hardened plates (scky‐outy bits such (scky‐outy bits). 2 long hair‐like tails. 3 long hair‐like tails, with the middle tail as small tubes, ny Has long, segmented NEMOURIDAE brush of bumps, or feathery tubular body. being somewhat shorter hairs ethan th outer tails. bits). Body is shaped OLIGOCHAETA like a bowling pin. BAETIDAE SIMULIIDAE

Hair‐like tails Feathery bits Hair‐like tails

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Water Quality Indicators

Mountain View School & Acterra Stewardship Watershed Education Project Funding from 2014 Clean, Safe Water Priority B Grant Program Spring 2016

ID to Family Level

 Phylum Class Order Family Genus Species

Chordata: Mammalia: Primates: Hominidae: Homo: H. sapiens

Soft body, but no hard calcium shell, no Soft body encased in a hard calcium shell segmented legs, no appendages Molluscs Worms

Pharynx Pea clam Eggs

Ramshorn Asian clam New Zealand Left‐handed snail Mudsnail snail Flatworm in alcohol Segmented worm Flatworm Fun Facts Fun Facts  Looking at the banding patterns – light and dark –on the  Flatworms only have one opening for eating and pooping. inside of the shell can tell you how old a clam is. Some clams  Flatworms were the first creatures to have a brain. Scientists can train live to be over 100 years old. flatworms to do simple tasks, such as finding water in a maze. When these  Many snails have both male and female parts and can self flatworms are then cut in half, the new halves learn faster than the original. fertilize, but they prefer to find another snail and exchange  Segmented worms can taste things with little wart‐like structures spread sperm. New Zealand Mudsnails are born pregnant. across their body.

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No segmented legs, yes appendages, No segmented legs, yes appendages, yes distinct head ‐ no distinct head ‐ some true fly larvae some other true fly larvae Crane Fly Dance fly PUPA LARVA CraneCrane FlyFly Dance fly ADULT Head

LARVA LARVA PUPA ADULT ADULT ADULT Fun Facts LARVA Midge fly Black fly • Those tentacles on the rear end of a crane fly larva are breathing structures. This means it can breathe air through its, Fun Fact um, bottom by sticking it out of the water.  Black flies will suck your blood. They are attracted to carbon  Some dance fly species have an elaborate courtship ritual in dioxide so if you stop breathing it will make it harder for them which the male wraps a prey item in silk and presents it to the to find you. female to excite her.

Yes, segmented legs, but more than six so they are not insects Yes segmented legs, but just six ‐ insects Abdomen ends in 2‐3 hair‐like “tails,” wing pads present

Arachnid: Water mite Crustacean: Scud Mayfly Stonefly

1 claw at tip of HEAD each leg 2 claws at tip THORAX Crustacean: HEAD of each leg Crayfish Gills on the rear Wing pads THORAX half of body ABDOMEN No gills on rear half of Usually 3 long, body hair‐like tails ABDOMEN (but sometimes 2 long, hair‐ like tails Fun Fact only 2)  Crayfish walk forward, but swim backwards.

Mayflies Stoneflies Triangular gills

Flat head Neck gills

NYMPH ADULT “Hairy” armpits NYMPH NYMPH Middle tail is NYMPH NYMPH shorter “Tails” shorter than abdomen ADULT NYMPH Fun Facts • Unique among insects, mayflies have paired genetalia: two Fun Fact penises or two gonopores… • Some species can even live in temporary streams; when the streams • …although females can lay fertile eggs without the males. dry out, they can temporarily suspend body growth and development, slowing down their metabolism in order to survive • The larvae may spend a year or so in the creek. The adults live until moisture is restored. for only a day or two and do not have functional mouths.

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Abdomen does not ends in 2‐3 hair‐like “tails” Abdomen does not ends in 2‐3 hair‐like Damselflies and Dragonflies “tails”, wing pads not present, abdomen is fleshy ‐ Caddisflies ADULT ADULT

Hardened plates on all three thoracic segments

Gills on ADULT abdomen LARVA Leaf‐like ADULT “tails” NYMPH NYMPH

LARVA Fun Facts  Dragonflies have been clocked at 60 miles per hour. LARVA Humps on sides of first Deep constrictions between  300 million years ago, dragonflies were as big as seagulls. abdominal segment abdominal segments

Caddisfly Cases Caddisfly Art

Hubert Duprat

Fun Fact  Many caddis flies build portable houses out of silk and a variety of creek materials – sand, rocks, algae, leaves, and pine needles. www.youtube.com/watch?v=pP0S1yZWvAw

Abdomen is not fleshy, wing pads not present Body is beetle‐like

ADULT ADULT Water boatman LARVA Riffle beetle Riffle beetle Water penny Fun Fact Really Fun Fact  The water boatman’s singing penis makes it the loudest animal in the world  Most water pennies can breathe oxygen from both water for its size. To make this sound which attracts female water boatmen, the and air. male scrubs his penis along ridges on his abdomen. The noise level reaches 99.2 decibels; louder than a freight train which is only 80 decibels.

3 H S BABY BUG BINGO Can You Name These Bugs? W E

P

H S W E P Benthic Macroinvertebrate Study Lab Protocols ‐ Sorting

GETTING THE BUGS OUT OF THE SAMPLE VIAL AND INTO THE SORTING TRAY:

 Take lid off bug sample vial.  Remove label with tweezers, squirt label with alcohol over sorting tray to remove stuck bugs, and set the label aside.  Place your thumb so that it completely covers the top of the vial, then turn the vial upside down and allow bugs to settle by your thumb. Swirl gently if needed.  Hold the upside down vial over the sorting tray and slowly remove your thumb so that the bugs and alcohol gently burble out into the sorting tray.  Squirt inside of vial with alcohol to remove any bugs still clinging to the sides.

SORTING THE BUGS FROM THE NON‐BUGS:

 Refill sample vial with clear alcohol  Using the tweezers, gently remove a bug from the sorting tray and place it down into the alcohol in the sample vial. Please DO NOT scrape the bug onto the lip of vial to dislodge it.  Do that for all the bugs, working for 30 minutes.  Place label back into the sample vial along with the bugs.

QUALITY CONTROL:

 Teams will shift one place over and look through the neighboring team’s tray for 5 minutes, removing any remaining bugs with tweezers and placing them gently in the vial.

CLEANING UP:

 Teams will return to their original vial.  Make sure the label is in the vial.  Securely screw the cap onto the vial. Replace the vial in your sample bag and zip it up.  Please DO NOT put the tweezers into the bag; leave them on the tabler fo next period’s team.  Pour leftover contents – debris and alcohol – from the sorting tray into the jar provided at your table. Please DO NOT dump this stuff down the sink.

H S W E P Benthic Macroinvertebrate Study Lab Protocols – Bug Identification

GETTING THE BUGS OUT OF THE SAMPLE VIAL AND INTO THE GLASS PETRI DISH:

 Take lid off bug sample vial.  Remove label with tweezers, squirt label with alcohol over sorting tray to remove stuck bugs, and set the label aside.  Place your thumb so that it completely covers the top of the vial, then turn the vial upside down and allow bugs to settle near your thumb. Swirl gently if needed.  Hold the upside down vial over the petri dish and slowly remove your thumb so that the bugs and alcohol gently burble out into the petri dish.  Squirt inside of vial with alcohol to remove any bugs still clinging to the sides.

IDENTIFYING THE BUGS:  Look at the preserved reference collection bugs available at the front of the classroom, or…  Work your way through the taxonomic key, or…  Use the pictures in the Bug Clubber’s Guide.  Hint: the bugs you are most likely to find have “likely” written next to tally area eof th data sheet.

ONCE A BUG IS IDENTIFIED:  Fill a small vial 2/3 full with alcohol.  Fill out an ID label with the scientific family name. Please use the special alcohol pen. The name will probably end in “ae”, for instance “BAETIDAE.” Place the label lengthwise into the vial so the writing is visible from the outside.  You have five small vials, but will probably have more than five different CA: Santa Clara Co. 16&17 SEP14 Stevens Ck, McClellan Ranch kinds of bugs. You can put more than one type of bug in a vial, just be sure MC1 ID by: ______you add the additional family names to the label by the numbers. 1) Baetidae  Pick the bug up with the tweezers and gently place it down into the alcohol 2) Hydropsychidae 3) Perlodidae in the small vial. Please DO NOT wipe the bug off onto the lip of the vial.  Put one tick mark on the data sheet next to the proper family name for each bug identified. It is probably best to assign one team member to be in charge of data entry for each lab period.  Do this for all bugs. All bugs must be counted. All bugs must have tick marks.

CLEANING UP:  Place plastic caps on all small vials. Be sure to click twice or they will pop off later and leak.  Use the eyedropper to suck up some of the alcohol from the petri dish and put it into the sample vial. Return all remaining unidentified bugs to the sample vial. Please DO NOT dump any of the bugs into the sink.  Pour any leftover alcohol from petri dish into the jar provided at your table. Please do not pour any of this into the sink either.  Place sample vial, small vials, labels, data sheet, and feedback sheet into plastic bag and zip.  Leave pen, pencil, tweezers, specimen box, petri dish, bug guide and taxonomic key on the table for the next class.

Taxonomic Key to Common Peninsula/South Bay Stream Invertebrates

KEY 1 So‐body enclosed within a hard Body not enclosed within a calcium shell (clam or snail) hard calcium shell.

Body enclosed Body enclosed in No obvious segmented Obvious segmented in single shell. two hinged shells (jointed)legs (jointed) legs. Examples include the bugs shown below. If your bug has Shell has slight concentric ridges. Shell is yellow‐green with segmented legs, Shape is more oval and has squarish thick concentric ridges. go to KEY 2 “shoulders.” Fingernail clam Shape has more sloping S “shoulders.” Asian clam C Squarish Sloping “shoulder” “shoulder”

Has no obvious appendages Has some appendages Shell whorl is flat, Shell whorl is spiral (scky‐outy bits). Has long, (scky‐outy bits such two‐dimensional. tubular body as small tubes, ny Ramshorn snail bumps, or feathery P structures). Examples Flaened body and Rounded include the bugs triangular head. body shown below. If your Alcohol causes the bug has appendages, body to curl and lose go to KEY 3 shape. Flatworm With pointy bit of shell With pointy bit of shell P up, opening is on the up, the opening is on the le. Le‐handed snail right. Pond snail Dead Alive L P

opening Body is not segmented Body is segmented. May look like worms you find in your garden, but thinner and more delicate. Has what looks like a nose, but is No mouthpart, “nose,” O actually a mouthpart on its head. on head. Nematode Proboscis worm M H

This key to families and other major groups is meant to be used by volunteers in the Peninsula/South Bay Area. Characters described are for local species and may not apply to the family as a whole. Many illustraons and descripons are borrowed from Merri and Cummins (1996), McCafferty (1998), Hauer and Lamber (2006), and Birmingham, et al (2006). Info: [email protected]

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Obvious Segmented Legs, Arthropoda (from KEY 1) KEY 2

Has 6 segmented Has more than 6 (jointed) legs segmented (jointed) legs

Has more than 8 segmented Has 8 segmented legs and round legs (Crustacea). pinhead sized body. Water mite A

Flaened top to boom, Looks like a ny Looks like a small lobster, looks like a “roly‐poly.” shrimp. White to 1‐14 inches. Has 10 legs Gray to brown. Sowbug pale yellow. Scud with claws on the front two. I (Amphipoda) Crayfish D

End of abdomen has 2 or 3 “Tails” are absent, very long, thin, hair‐like “tails,” short, spiny, thickened, not paddle‐like. Examples or paddle‐like. Examples include the bugs shown include the bugs shown below. If your bug has 2‐3 below. If your bug has Body and head Body and head not markedly hair‐like tails, tails as described, flaened from flaened from side to side. side to side Antenna 1 slender, antenna 2 go to KEY 4 go to KEY 6 very large and robust. C

1

2

Antenna 1 and antenna 2 about Antenna 1 longer Antenna 1 shorter the same length. Small appendage than antenna 2. than antenna 2. extending from the base of the C H third segment of antenna 1. G

1 1 2 appendage 2

1 1 2 2

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Has some appendages—small tubes, ny bumps, or feathery structures (from KEY 1) D, T F KEY 3

Has what appears to be 7 segments, More than 7 segments and no sucker‐discs. lower surface has 6 prominent sucker‐ discs. Net‐winged midges B Does not have a disnct Has a disnct head with a head or head is retracted hardened head capsule. into thorax.

Head is small and Head retracted into pointy with lile rods thorax. Welts/prolegs Body is long Body is torpedo‐ Body shaped like of hardened material may be present on and cylindrical. shaped, head is bowling pin; two ny inside. In most spe‐ some abdominal seg‐ small, bristles oen feathery structures on cies, prolegs are pre‐ ments. Crane fly present on body. smaller head end. sent on all abdominal T Soldier fly Black fly segments. Dance fly S S E head

head prolegs head

feathery structures

One pair of prolegs Body is very Body is somewhat Head has mouth Body is divided on first body slender and under transparent. brushes. Abdo‐ into annuli/rings. segment. Body is 1/2 inch long. No Antennae have long men ends in a Dark rectangular 1/4 to 1/2 inch long prolegs. hairs. Last segment breathing tube bands on back. and may be some‐ Bing midge of abdomen has a and paddle‐like Abdomen ends in what transparent. C fan. Phantom structures. dark‐colored Midge fly midge/glassworm Dixid midge breathing tube. C C D Moth fly P mouth

brushes

proleg dark antennal bands hairs

abdominal fan breathing tube breathing tube

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End of abdomen has 2‐3 long, thin hair‐like “tails,” not paddle‐like (from Key 2). E, M P, S KEY 4

Sides of abdomen have plate‐ No gills on abdomen, like feather‐like, or leaf‐like one claw two claws or only on the first gills. Abdomen usually ends in few abdominal seg‐ 3 “tails,” but may be 2. Legs ments closest to are pped with 1 claw. Exam‐ gills at sides no gills at sides thorax. Always has 2 of abdomen ples include the bugs shown of abdomen “tails.” Legs are below. If your bug is as de‐ pped with 2 claws. 3 “tails” Always 2 scribed, go to KEY 5 (somemes 2) “tails”

No branching gills on Tus of gills on underside of thorax underside at base of legs. at base of legs, “hairy armpits.”

Underside of abdomen has tus No tus of gills on first 2 or 3 of gills on first two or three seg‐ abdominal segments—just at the ments. About 2 inches long. base of legs. Nymphs have handsome Dark and prehistoric looking. brown and yellow paerns on their Giant stonefly head and thorax. Common stonefly P P

Tus of gills at base Tus of of legs gills at base of legs

Tus of gills on first 2‐3 No tus of gills abdominal on first 2‐3 segments abdominal underside segments underside

Robust, hairy. Branched gills pre‐ Abdomen is long and widest in the “Tails” longer than abdomen. Inner sent on underside at “neck.” middle. “Tails” shorter than abdo‐ margins of lower wing pads form a Wing pads are divergent. men. Hairs on tails point away from “V.” Bodies oen highly paerned. Broadback stonefly abdomen. Green stonefly Paern stonefly N C P

neck gills Wing pads form a “V”

underside

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Sides of abdomen have plate‐like, feather‐like, or leaf‐like gills. Usually three tails, but may be two. Legs are pped with one claw (from KEY 4). KEY 5 E, M

First gills large and forming a cover First gills not large and form‐ of other gills, not meeng in center, ing a cover of other gills. and somewhat triangular in shape. Lile stout crawler mayfly T Generally delicate Generally robust body, gills body. Gills present on absent on abdominal segments abdominal segments 1 and 2. Spiny crawler mayfly 1‐7 or 2‐7. E

No gills on segments 1 and 2

large triangular gill

Eyes along outside edge of head Eyes on top side of head. Head and when viewed from above body disnctly flaened. Legs sprawling. Some think he looks like “Jack Skellington” in The Nightmare Before Christmas. 2 or 3 hair‐like 3 hair‐like “tails” with the middle tail Gills on abdomen forked “tails.” Flat‐headed mayfly being somewhat shorter and thinner or ending in filaments. than the outer tails. Gills are oval‐ Pronggill mayfly H shaped. Antennae 2‐3 mes head L width. Small minnow mayfly B

side view side view—see, he is really, really flat.

forked gill

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“Tails” are absent, very short, spiny, thickened, or leaf‐like (from KEY 2)

KEY 6

Abdomen with well‐developed thread‐ Abdomen without well‐developed thread‐like like appendages along sides. appendages along sides (some gills may be present).

Wings or wing Wings or Abdomen ends in Abdomen ends with 2 Abdomen ends with 4 single, long “tail.” appendages, each with hooks. Legs pped with pads not present. wing pads (Megaloptera) 2 claws. (Megaloptera) 2 claws. (Coleoptera) present. Alderfly Dobsonfly Whirligig beetle S C G Abdomen Abdomen fleshy. hardened. Examples include Coleoptera the bugs shown

larvae below. If your bug is 2 claws as described, go to KEY 7

4 hooks 2 claws

Body is Body is not Body disc‐like with Abdomen tapers Body is hard, not fleshy. beetle‐like. beetle‐like. plates covering head strongly to p; two Segments have a shal‐ Extensible and legs. Water penny feathery tails. Preda‐ low groove on each hinged mouth P cious diving beetle. side. Riffle beetle parts. D E

lower side upper side Body usually thick‐ Body usually slender,

ened, no leaf‐like 3 long leaf‐like “tails.” Dragonfly “tails.” Damselfly Front pair of wings paerned Front pair of wings form or leathery without a center a hard, dark, oen shell‐ G C dividing line. Front legs short like covering with a di‐ In this family, antennae In this family, the first with front foot forming a viding line down center have four segments and segment of antennae is scoop (Hemiptera). of back (Coleoptera). are small and club‐like. the same size as the rest For other families use of the antennae segments. Water boatman Riffle beetle addional keys. For other families, use C E addional keys.

scoop‐shaped front leg

club‐like antenna

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Abdomen fleshy (from Key 6) KEY 7 T, C

3 hardened plates on upper side 2 or fewer hardened plates on of thoracic segments. upper side of thoracic segments.

Abdomen is strongly curved Abdomen has no branched Humps present on No humps present and has branched gills and gills. Larva is very small, about back and/or sides of on back or sides of scaered hairs. Brush of 1/16 in. Middle secon of ab‐ first abdominal seg‐ first abdominal hairs at end of abdomen. domen tends to be expanded. ment. Look carefully as segment. If your No case, but may make a May be in so pouch‐like case they may be small, bug is as described, rough “retreat” from small made of silk and fine sand par‐ clear and squished. go to KEY 8 rocks. Common netspinner cles. Micro caddisfly H H

3 hardened plates 3 hardened plates

no humps branched humps abdominal gills on underside no abdominal gills

brush of hairs case

Humps present on both the top and the Humps are present only on the sides sides of the first abdominal segment. of the first abdominal segment, not the top. Head is dark‐colored with pale spots. Front legs are shorter than middle and hind legs. Young larvae Front legs are slightly shorter Head is scrunched in between first build cases of sand, older larvae build than middle and hind legs. pair of legs. T‐shaped plate on log‐cabin style cases with vegetaon. Northern caddisflies under side of head. Darkened Lile brown sedges L ovals on under side of abdomen. L Larvae build cases from coarse rock parcles. U

humps humps

humps

case view from above case

view from above case

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No humps present on back or sides of first abdominal segment. (from Key 7) T, C () KEY 8

Abdomen is elongated and Abdomen is stubby and tapers tapers significantly towards only slightly towards the end. the end. Well‐developed Small claws at end of abdomen. claws at end of abdomen.

Hardened plate on upper side of first Body disncve with dark hardened plates thoracic segment divided by a on head, legs, and top of first thoracic seg‐ crease. Pale colored head with dark ment; underside of first thoracic segment spots. Front legs are shorter than has two hardened plates which look a bit middle and hind legs. Larvae build like a bra. All legs are all about the same cases of filamentous green algae. length. Case is shaped like a saddle or turtle Humpless cased caddisfly shell—humped on top and flat on the B boom. Saddle‐case makers G hardened plates crease

case

Large body with deep constricons Head is elongated, enrely the Hatched‐shaped structure ex‐ between segments (resembles same color—no paerns. Mouth tending between head and first Michelin Man). Abdomen tends to curl extension so, white, T‐shaped— leg. No case. Neube caddisfly in alcohol. All 3 sets of legs are about may be retracted, but will pop out P the same length. Very large claws at if head is pressed. First thoracic end of abdomen. No case. Free‐living segment has hardened plate caddisfly which has a black band at the rear R edge. No case. Fingernet caddisfly P deep constricons between segments black band

Large claws at end of abdomen T‐shaped mouth hatchet‐shaped extension structure

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General Bug Informaon

Basic Bug Bits Bug Vocabulary

Abdomen—rear end secon of body M behind the thorax.

Antennae—slender, movable sensory organs at head of bug. Appendage—part of body which scks T out. Gills—Breathing structures (quite R oen leaf‐ or feather‐shaped). M Head—secon of body in front of the thorax. Contains the mouth parts and major sense organs.

Segmented leg—true leg; jointed C (hinged) leg capable of bending. D Larva—juvenile or immature life stage. Pupa—life stage of some insects in

which they undergo transformaon from larva to adult. Proleg—short, stumpy leg‐like struc‐ tures (not jointed) Segment—a secon of the body. Tails—hair‐like, bristly, or paddle shaped structure(s) extending from end of abdomen.

Thorax—secon of body between the head and the abdomen. Segmented legs and wings/wingpads (if present) are always aached to the thorax. Wing pads—underdeveloped wings, oen in “W” shape.

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World Water Monitoring Challenge Field Data Sheet

Creek: ______

Monitoring site name: ______Date: ______Monitor’s name: ______Time: ______

O: Circle one underlined option: Cloud cover no clouds; partly cloudy; cloudy sky Precipitation none; misty; foggy; drizzle; rain Wind calm; breezy; windy Murkiness clear water; cloudy water (>4" visibility); murky (<4" visibility) Flow full waterway‐no observed flow; heavy; medium; light; trickle; isolated pools; dry Color none; amber; yellow; green; brown; gray; other Sample odor none; fresh algae smell; chlorine; rotten eggs; sewage; other Other algae or water plants; oily sheen; foam or suds; trash; other

M:

Temperature °C Dissolved Oxygen ppm (mg/L) pH Turbidity JTU (NTU)

B “ ” :

Group One Group Two Group Three Somewhat pollution tolerant Pollution sensitive organisms found Pollution tolerant organisms can be organisms can be in good or fair in clean water. found in any quality water. quality water. Mayfly (most) Riffle Beetle Mayfly (Baetidae Family) Stonefly Scud Sowbug Caddisfly (most) Water Mite Black Fly Water Penny Crane Fly Midge Fly DobsonflyCaddisfly (Hydropsy- Segmented Worm chidae Family) Flatworm Dragonfly Clam Alderfly Snail Damselfly Water Boatman

Soap from washing cars in the driveway, ferlizers or pescides applied to gardens, fluids which drip from cars, and pet waste all wash into the storm drains and enter local creeks causing polluon.

InstrucƟons for StaƟon Supervisor

World Water Monitoring Challenge Turbidity

Turbidity is a measure of the cloudiness or murkiness of the water due to suspended fine sediment, small bits of organic or inorganic maer, and microscopic organisms. Carrying sedi- ment—both large and fine—is a natural funcon of a creek, but the amount of suspended fine sediment in many local creeks can be greatly increased due to:

 Dam/reservoir management  Quarrying acvies  Urban runoff from residences and businesses through storm drains to the creek  Soil erosion due to road cuts, construcon, foot/bicycle/car traffic on unpaved areas  Boom-feeding, nonnave fish such as carp

Excess turbidity is regarded as a serious pollutant as it may cause aquac animals to have difficulty seeing and breathing. Turbidity reduces biodiversity—the number of species which can live in an area. Furthermore, the fine sediment which causes the turbidity eventually seles to the boom of the creek where it may cause further damage as it smothers fish eggs and boom dwelling bugs.

To give the parcipants a beer idea of what excess turbidity is like for animals that live in the creek, ask them how they would feel if the air around them were full of fine sediment. They can imagine how hard it would be for them to see and breathe, and that they might have health problems.

Turbidity Procedure

 Have volunteers look at the Secci disk before they put water in the jar.  Fill the white jar with creek water to the turbidity line located on the label.  Hold the turbidity chart on the top edge of the jar.  Looking down into the jar, compare the appearance of the Secchi disk icon at the boom of the jar to the chart.  Record the result on the data sheet. Unless it has rained recently, the water from a creek with no human influences should generally be very clear.

Just for fun, the volunteers then might want to add a bit of dirt—a sort of mini-erosion—from the stream bank to the water in the jar and observe the result.

InstrucƟons for StaƟon Supervisor

World Water Monitoring Challenge Dissolved Oxygen

Dissolved Oxygen (DO) is very important to the health of aquac ecosystems. All aquac ani- mals need oxygen to survive just like we do. Natural waters with consistently high dissolved oxygen are capable of supporng a diversity of aquac organisms. The amount of oxygen dissolved in the water can vary for many reasons:

 Photosynthesis by water plants raises DO, while respiraon processes lower DO. Therefore, DO is higher during the day than during the night and higher on sunny days than cloudy days.

 High levels of bacteria or rong plants can cause DO to drop. For instance, ferlizers running off residences through storm drains to the creek can cause excess algal growth. When this algae dies, it consumes oxygen and can cause DO to drop.

 In creeks with a dam, the reservoir can have a great effect on DO in the creek for about a half mile downstream. The reservoir becomes strafied during the summer with lile mixing between the upper and lower levels. The lower level becomes oxygen deficient due to the inability of light to penetrate and photosynthesis to take place. With dams with a boom release, it is this oxygen deficient water which is pumped in to the creek below.

 Turbulent water flows, such as occurs in riffles, incorporate more oxygen into the water.

 Cold water can hold more dissolved oxygen than warm water. If the water becomes too warm, there may not be enough oxygen for more sensive animals such as Steel- head or rainbow trout. Ask parcipang volunteers if they have ever been at a high altude where the air was thin so that they had to gasp for breath and got red quickly. This is what happens when oxygen lev- els are low. Older volunteers may wish to know that the saturaon percentage of dissolved oxygen is also an important measurement of water quality. Cold water can hold more dissolved oxygen than warm water. For example, water at 28°C will be 100% saturated with 8 ppm dissolved oxygen. However, water at 8°C can hold up to 12 ppm of oxygen before it is 100% saturated.

Dissolved oxygen procedure on reverse side.

Dissolved Oxygen Procedure

 Fill the white jar with creek water.  Submerge the small vial into the water sample. Carefully remove the vial from the water sample, keeping it full to the top.

 DO TesTabs are toxic and should only be handled with gloves.  Drop two DO TesTabs into the vial. Water will overflow when the tablets are added. Volunteers should not touch the toxic TesTabs with their hands.  Screw the cap on the vial. More water will overflow as the cap is ghtened. Make sure no bubbles are present in the sample.  Mix by inverng the vial over and over unl the tablets have disintegrated. This will take about four (4) minutes.  Wait five (5) more minutes for the color to develop.  Compare the color of the sample to the Dissolved Oxygen color chart.  Record the result as ppm (parts per million) or mg/L (milligrams per liter). These are equivalent measurements. A healthy reading would be 8 mg/L.

 Do not pour contents of the test tube into the creek. Please use the waste disposal boƩle.

 Older parcipants may also wish to Dissolved Oxygen compute the percent saturaon of 0 ppm 4 ppm 8 ppm DO. Cold water can hold more DO than 20 2958 warm water. Locate the temperature 40 3161 60 3264 of the water sample on the chart. 80 3468 Locate the dissolved oxygen result of 10 0 35 71 Temperature°C the water sample at the top of the 12 0 37 74 14 0 39 78 chart. The % saturaon is where the 16 0 41 81 temperature row and the dissolved 18 0 42 84 20 0 44 88 oxygen column intersect. For example: 22 0 46 92 if the temperature is 16°C and the 24 0 48 95 26 0 49 99 dissolved oxygen is 4 ppm, the 28 0 51 102 % saturaon is 41. 30 0 53 106

InstrucƟons for StaƟon Supervisor

World Water Monitoring Challenge pH

pH is a measurement of the acidic or basic quality of water. The pH scale ranges from a value of 0 (very acidic) to 14 (very basic), with 7 being neutral. Healthy creeks usually have a pH of between 6.5 and 8.5. The pH of our local creeks is naturally a bit more basic than many creeks due to the geology of the upper watershed - much limestone, or calcium carbonate, is present. Our wildlife is adapted to this higher range. Depending on the season and the relative amounts of water either flowing into the creek from storm runoff or seeping into the creek from groundwater, the pH tends to range from 7.2 to 8.5 locally. Other factors which can influence pH in local creeks are:

 Pollutants entering the creek from residenal runoff.

 Sewage contaminaon. You can ask older volunteers if they know within what range their blood pH needs to be. It is 7.35 to 7.45, a very narrow range…and comparable to creek pH. Why is pH so important, both in the creek and in our bodies? Chemical reacons are very pH sensive. If the pH is too high or too low, the chemical reacons needed to maintain health cannot take place.

pH Procedure

 Fill the test tube to the 10 mL line with the water sample.

 Add one pH Wide Range TesTab.

 Cap and mix by inverng unl the tablet has disintegrated. Bits of material may remain in the sample.

 Compare the sample to the pH color chart.

 Record the result as pH.

 Do not pour contents of the test tube into the creek. Please use the waste disposal boƩle. If the volunteers are interested, you may show them the premade vials which contain acidic (vinegar) and basic (borax) materials, and compare them to the color chart.

InstrucƟons for StaƟon Supervisor

World Water Monitoring Challenge Temperature

Aquac animals, such as steelhead/rainbow trout and creek bugs, are sensive to changes in water temperature and require a certain temperature range to survive and thrive. In addion, temperature affects the rate of photosynthesis by aquac plants and the sensivity of organ- isms to toxic wastes, parasites, and disease. Temperature also affects the amount of oxygen water can hold, and all animals need oxygen to survive. Steelhead/rainbow trout have rela- vely high oxygen needs. Water temperature in local creeks may be affected by:

 The containment of water in a reservoir behind a dam. During the cool months, creek water below a dam tends to be warmer than creek water above a dam, while the opposite is true during the summer.

 The removal of trees and vegetaon which shade the creek can raise the water temperature.

 The residenal or business use of ground water from wells can prevent cool groundwater from seeping into the creek. Ask your volunteers what temperature their body should be. What happens if their tempera- ture is too high or too low? Chemical reacons are very temperature sensive. If the tempera- ture is outside a fairly narrow range, the chemical reacons needed to maintain health cannot take place.

Temperature Procedure

 Place the thermometer four (4) inches below the surface for one (1) minute.

 Remove from the water.

 Read the temperature and record the result as degrees Celsius.

InstrucƟons for StaƟon Supervisor

World Water Monitoring Challenge Biomonitoring Benthic Macroinvertebrates, Creek Bugs

While regular monitoring of water chemistry—temperature, pH, dissolved oxygen, and turbidi- ty—provide important information on water quality, it provides only part of the information needed to assess habitat quality. Biomonitoring of organisms such as benthic macroinverte- brates, the organisms without a backbone which dwell at the bottom of the creek, gives a more complete picture. Insect larvae, worms, clams, and snails are some of the more common macroinvertebrates or “bugs” found in a creek and vary in their tolerance to pollution. The relative abundance or scarcity of certain benthic macroinvertebrates at the time of collection can tell us much about what aquatic habitat quality has been at that site for weeks or even months prior to collection.

The larvae from the insect orders Ephemeroptera, Plecoptera, and Trichoptera, better known as mayflies, stoneflies, and caddisflies, are particularly sensitive to the effects of pollution, fine sediment, low dissolved oxygen, and so forth. Finding a lot of these bugs can indicate a healthy habitat. On the other hand, finding a lot of pollution-tolerant bugs, such as blackfly larvae, midge fly larvae , and aquatic worms, can indicate a pollution problem.

Biomonitoring Procedure

 Encourage volunteers to take a few minutes to see how many different creek bugs they can find in the tray. Tell them that some of the bugs may be very, very small.

 Aer the volunteers have seen that there are many different sorts of bugs, encourage them to use a “cheat sheet” to idenfy the bugs. Tell them which clues to idenficaon to look for: general body shape, presence/number of legs, presence/locaon of gills, presence/shape of tail, and so forth.

 Have the volunteers enter on the data sheet the bugs they idenfy. They may count the number of bugs of each type if they wish.

 Ask them to give an opinion on the quality of the habitat based on these bugs. Explain that it is not only the type of bugs they find, but also the diversity of bugs which provides clues to habitat health.