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Rabbit Anti-RAPH1/FITC Conjugated antibody

SL21158R-FITC

Product Name: Anti-RAPH1/FITC Chinese Name: FITC标记的RAPH1蛋白抗体 9430025M21Rik; Amyotrophic lateral sclerosis 2 chromosomal region candidate 18 ; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 Alias: protein; C730009O10Rik; Lamellipodin; PREL-2; Proline-rich EVH1 ligand 2; Protein RMO1; RAPH1; RAPH1_HUMAN; Ras-associated and pleckstrin homology domains- containing protein 1. Organism Species: Rabbit Clonality: Polyclonal React Species: ,Mouse,Rat,Dog,Pig,Cow,Rabbit,Zebrafish,Sheep, ICC=1:50-200IF=1:50-200 Applications: not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Molecular weight: 135kDa Form: Lyophilized or Liquid Concentration: 2mg/1ml immunogen: KLH conjugated synthetic peptide derived from human RAPH1 Lsotype: IgGwww.sunlongbiotech.com Purification: affinity purified by Protein A Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year Storage: when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Function: Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion. Product Detail: Subunit: Interacts with EVL and VASP and targets them to the leading edge (PubMed:15469845). Interacts (via Ras associating and PH domains) with RAC1 (PubMed:18499456).

Subcellular Location: Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cytoplasm > cytoskeleton. Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.

Tissue Specificity: Isoform RMO1-RAPH1 is ubiquitously expressed with highest levels in brain, heart, ovary and developing embryo. Isoform RMO1 is widely expressed with highest levels in liver. Low expression in B-cells.

Similarity: Belongs to the MRL family. Contains 1 PH domain. Contains 1 Ras-associating domain.

Database links:

Entrez Gene: 65059 Human

Omim: 609035 Human

SwissProt: Q70E73 Human

Unigene: 471162 Human

Important Note: This product as supplied is intended for research use only, not for use in human, therapeuticwww.sunlongbiotech.com or diagnostic applications.