Monoclonal Anti-NCAPH2 antibody produced in rat, clone 5F2G4, purified from hybridoma cell culture

Catalog Number SAB4200655

Product Description Precautions and Disclaimer Monoclonal Anti-NCAPH2 (rat IgG2a isotype) is derived This product is for R&D use only, not for drug, from the hybridoma 5F2G4 produced by the fusion of household, or other uses. Please consult the Safety mouse myeloma cells and lymph node cells from rat Data Sheet for information regarding hazards and safe immunized with a human NCAPH2 (full-length) handling practices. recombinant fusion ( ID: 29781). The antibody is purified from culture supernatant of Storage/Stability hybridoma cells grown in a bioreactor. For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in Monoclonal Anti-NCAPH2 recognizes human mouse “frost-free” freezers, is not recommended. If slight and rat NCAPH2. The product may be used in several turbidity occurs upon prolonged storage, clarify the immunochemical techniques including immunoblotting solution by centrifugation before use. Working dilution (~95 kDa), immunofluorescence and flow cytometry. samples should be discarded if not used within 12 hours. NCAPH2 (Non-Structural Maintenance of II complex subunit H2), also known as Product Profile Chromosome-associated protein H2, Cap-H2 or Immunoblotting: a working concentration of 2-4 µg/mL Kleisin-beta, plays a crucial role in chromosome is recommended using whole extracts of HeLa cells. assembly and segregation.1-3 Immunofluorescence: a working concentration of It belongs to a superfamily of termed kleisins.1 0.25-0.5 µg/mL is recommended using HeLa cells. NCAPH2, along with NCAPD3 and NCAPG2 are unique components of the ring-like Condensin II Flow Cytometry: a working dilution of 1-2 µg/test is complex that also contains the SMC2/SMC4 recommended using HeLa cells. heterodimer. (I and II) are major players in chromosome dynamics in mitotic and meiotic cells. Note: In order to obtain the best results using various In Drosophila Condensin II complex induces axial techniques and preparations, we recommend compaction of during interphase, determining optimal working dilutions by titration. Use disrupts interchromosomal interactions, and promotes of sensitive film is recommended. the dispersal of peri-centric heterochromatin.2 In addition, NCAPH2 was also reported to be involved in References mammalian T cell differentiation, but not in B 1. Ono, T., et al., Cell, 115, 109-121 (2003). lymphocyte.3 2. Christopher, R.B., et al., PLoS Genet., 8, e1002873 (2012). Reagent 3. Gosling, K.M., et al., Proc. Natl. Acad. Sci., 104, Supplied as a solution in 0.01 M phosphate buffered 12445–12450 (2007). saline, pH 7.4, containing 15 mM sodium azide as a preservative. TD,PHC 05/16-1

Antibody Concentration: ~ 1.0 mg/mL

2016 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.