Annual Report 2003

ISSN 1334-854X ISSN 1334-854X

RRuðeruðer BBoškoviæoškoviæ IInstitutenstitute AnnualAnnual reportreport 20032003

Ruðer Boškoviæ Institute

Annual report 2003

Arial view of the RBI campus

Zagreb, 2004. Editorial Board: Editor: Nela Pivac

Members: Stjepko Faziniæ Stjepan Marèelja Iva Melinšèak Zlodi Karolj Skala David Smith Neven Žarkoviæ

Technical Support: Photographer: Josip Uhrl Graphic Design: Faculty of Graphic Arts, University of Zagreb Printed by: Kratis d.o.o. Print run of 1500 copies Web edition: www.irb.hr/annualreport

Address: Ruðer Boškoviæ Institute Bijenièka cesta 54, P.O.Box 180 HR-10002 Zagreb, Croatia

Contact: URL: http://www.irb.hr e-mail: [email protected] tel: +385 1 45 61 111 fax: +385 1 46 80 084

Introduction...... 1

Education...... 5

RBI Bibliography...... 7

Theoretical Physics Division...... 11

Division of Experimental Physics ...... 15

Division of Materials Physics...... 21

Division of Laser and Atomic Research and Development...... 25

Division of Electronics ...... 27

Division of Physical Chemistry ...... 31

Division of Organic Chemistry and Biochemistry ...... 35

Division of Materials Chemistry...... 41

Division of Molecular Biology ...... 45

Division of Molecular Medicine...... 51

Division of Marine and Environmental Research ...... 57

Center for Marine Research...... 63

Center for Informatics and Computing ...... 69

Center for Nuclear Magnetic Resonance ...... 73

Library...... 75

RBI annual report 2003. 1 competitive selection procedure, based solely on the criterion of excellence, resulted in these posts being filled with scientists ranging in age from 30 to 37. In addition, three further positions, also at the level of research associate, were approved for Croa- tian nationals returning from distinguished stays , at the level of lecturer or Physics Another step in the right direction was Another step in the right direction and Sport). As this is ordinarily the primary As this is and Sport). personnel at the Institute, source of young necessarily slowed. In an progress was this problem, the Insti- attempt to help rectify use its own reserves, com- tute decided to received by research bined with funding scientific work, to support a groups for their students. In a positive group of 17 graduate financial but partial corrective step, national the students support was granted to some of towards from this internally supported group, the end of the year. This 2003. also made towards the end of new came in the form of around two hundred reform positions nation- research associate, made available The positions were proportionally divid- ally. institutions in the coun- ed between different A posts. try so that the RBI received 18 new Geoscience Chemistry Composition of Researchers at the RBI

Figure 1.

In some respects, 2003 was a difficult In some respects, 2003 was a Welcome to the new format of the annual to the new format of the Welcome Introduction Bioscience Computer science and engineering year at the Institute. This was primarily due year at the Institute. very few to the fact that, throughout Croatia, approved graduate student positions were Technology by the Ministry of Science and Education (presently the Ministry of Science, report. This year, rather than providing a rather than providing This year, report. report of all work at the comprehensive Institute (RBI), we wish to Ruðer Boškoviæ information and examples present selected the activity of the RBI in its that will illustrate Institute strives to The main areas of work. in all aspects of its improve the performance signifi- mission and during 2003 we made cant advances. Some of the achievements while more will be apparent from this report, from the details will be available shortly on the comprehensive report to be posted institute web pages. 2 RBI annual report 2003. values andvision. upholding thesame tions anduniversities many researchinstitu- RBI collaborateswith Area. Worldwide, the European Research forms apartofthe pean Union,theRBI stry. WithintheEuro- technology-based indu- governments andto nity, tostateandlocal the academiccommu- provision ofsupportto higher educationand dedicated toresearch, is anationalinstitution Within Croatia,theRBI Figures 1and2). mental research(see and marineenviro- biology, biomedicine physics, chemistry, dents workinmanydifferent fieldsincluding than 500academicstaff andgraduatestu- plinary environmentoftheInstitute,more and scienceapplications.Inthemulti-disci- largest Croatianresearchcentreinsciences ful careerattheRBI. new colleaguesaswewishthemasuccess- to beableoffer awarmwelcometoour is muchimprovedandweareverypleased ments, thescientificstrengthofInstitute at foreigninstitutions.Withtheseappoint- The RuðerBoškoviæInstituteisthe OVERVIEW Figure 2. Chairman oftheBoardGovernors: Head oftheScientificCouncil: Director: ORGANIZATION OFTHEINSTITUTE agement) toencompasstheareasof ate directorships(aspartofExecutiveMan- These includedthecreationofthreeassoci- tional structurewereintroducedin2003. Institute, severalnewelementsoforganiza- to improvetheoverallperformanceof Institute isdepictedinFigure2.Inaneffort The organizationoftheRuðerBoškoviæ Organizational StructureofRBI Stjepan Marèelja Krunoslav Pisk Ivo Šlaus RBI annual report 2003. 3 The Science (STP). Several Agreement of Collabora- For the first time ever, scientists form the scientists time ever, For the first The Business Plan includes a short-list of The Business Plan includes a short-list a Despite the fact that the RBI is primarily Applied Science, Technology and Technology Science, Applied Management Business Director for Associate lead by the Institute, wrote the Busi- Development, Commercial This period 2004-2008. ness Plan for the the supervision of experts was done under Ministry of Science and engaged by the Bank in the context of the World Technology entitled restructuring program and Technology Project and Technology Business Plan were written drafts (28) of the that the final version will and it is expected be accepted in 2004. an origi- 12 applied research projects (from evaluation nal 46) selected through an committee process conducted by an internal experts, as well as international technical Advisor (Ian including the RBI's Institutional (Ramesh Cooper) and foreign experts During the Mashelkar and Stewart Craig). have been first phase, four of these projects sup- selected to be funded (with a planned con- port of up to $0.5 million for equipment, basis. sultants and services) on a pilot Contribution to higher education research institution, our participation in educational activities during 2003 was strong. Scientists from the Institute contributed 39 courses at the undergraduate level as well as 186 postgraduate courses. In addition, 27 B.Sc., 23 M.Sc. and 28 Ph.D. theses were completed under the supervision of the Insti- 2003 saw Importantly, tute's academic staff. the signing of an three circled members. A of pentaquark hypothetical antidecuplet Overall, 2003 saw an increase in the Overall, 2003 saw an increase Despite the somewhat difficult circum- difficult Despite the somewhat ACTIVITIES ACTIVITIES manifestly exotic and cannot be made of three quarks. Experiments now see candidates for the Figure 3. baryons. The states at the corners of the triangle are number of research articles published in number of research articles published This journals cited by Current Contents. a 32 % year's total of 408 represents articles pro- improvement over the 310 such duced in 2002. stances apparent throughout 2003, the Insti- stances apparent many significant scientific tute achieved The most important achievement advances. the discovery of two new of the year was a large within Working five-quark states. the CERN international collaboration at small group Super Proton Synchrotron, a Antièiæ, Tome from the RBI, consisting of Šuša, analysed Tatjana Krešo Kadija, and two new the data in Zagreb and identified Figure 3). heavy pentaquark baryons (see Fundamental research Science (Neven Žarkoviæ), Commercial Žarkoviæ), (Neven Science and Etlinger), (Božidar Development (Jaroslav Horvat). Finance 4 RBI annual report 2003. third consecutiveyear. As partofthispro- major equipmentpurchasesoperatedforthe New InstrumentationandInfrastructure different proposeddevelopments. evaluation oftheenvironmentalimpact and nationalauthorities,foradviceonthe Institute areoftencontacted,bybothlocal tion acrossthecontinent.Expertsfrom and disseminationofenvironmentalinforma- countries, alsocontributestothecollection tional collaborationswithotherEuropean the air. The RBI,throughthemanyinterna- both thelevelofradiationandquality in nationwideprogramsforthemonitoringof waters intheregion. They arealsoinvolved knowledge ofthe Adriatic Seaandinland research divisionscontributestronglytoour Environmental researchandconsulting ular Medicine the newjointfieldofdoctoralstudyin experts fromtheRBIwerechosentolead Zagreb MedicalFaculty. Inthiscontext, tion In 2003,therevivednationalprogramof The Institute'stwoenvironmental between theRBIandUniversityof . Molec- vate NetworkandGridapplications. the demandingrequirementsforVirtual Pri- 3100 connections. This new LANsatisfies Layer-3 and31Layer-2Ciscoswitcheswith between 26buildingsandiscontrolledby2 sists of5kmfibre-opticcablestretching overhauled in2003. The new networkcon- local areacomputernetwork(LAN)was costs. in doingso,partiallyoffset theoperational ity withourrelativelynewNMRfacilitiesand, commercial usersmadeuseofthispossibil- cially iftimeisavailable.Indeed,several ment usecanalsobeleasedoutcommer- tia atthenominalrunningcost. The equip- all qualifiedusersfromtheRepublicofCroa- stood asanationalresourceandisopento tute. research possibilitiesavailableattheInsti- tribute significantlytotheexpansionof instruments willbeusedwidelyandcon- USA). Itisexpectedthatbothofthese 18 sputteringsystem(KurtJ.LeskerCo,PA, scope (Leica TCS SP2 AOBS) andaCMS- enabling thepurchaseofaConfocalmicro- gram, theRBIreceivedamajorgrant In additiontotheabovepurchases, All majorinstrumentationatRBIisunder- Education

In 2003, the RBI continued with the ronmental Research (8) (Figure 5). expansion of its educational activities. The The postgraduate (doctoral) studies were on-site education of young scientists result- also primarily conducted in collaboration ed in 27 B.Sc., 23 M.Sc., and 28 Ph.D. the- with the University of Zagreb (Figure 6). Of ses. Formal teaching consisted of a total of the 160 such courses held during 2003, 109 39 undergraduate courses and 186 post- were courses taught in collaborative pro- graduate courses taught on behalf of Croat- grams with the Faculty of Science, Universi- ian universities. ty of Zagreb. The Medical School, Universi- The majority of the undergraduate cours- ty of Zagreb, was our second largest partner es taught by teachers from RBI (29/39) (25 courses) followed by the University of resulted from our links with the University of Osijek (14 courses). One course of special- Zagreb (Figure 4). The Division of Molecular ized postgraduate study was taught at the Biology, in collaboration with the Faculty of Budapest University of Technology and Eco- Science, was the most active (12 courses), nomics. As can be seen from Figure 7, the followed by the Division of Physical Chem- most active Divisions in postgraduate edu- istry (9) and the Division of Marine and Envi- cation were the Division of Marine and Envi-

Figure 4. Distribution of the RBI held undergraduate courses by domicile University B nulrpr 2003. report annual RBI

Figure 5. Distribution of the RBI held undergraduate courses by Division 5 6 RBI annual report 2003. doctoral studyin were chosentoleadthenewjointfieldof Zagreb. Inthiscontext,expertsfromtheRBI RBI andtheMedicalSchool,Universityof Agreement ofCollaboration courses). and theDivisionofMolecularBiology(29 sion ofMolecularMedicine(43courses), ronmental Research(48courses),theDivi- between RBIanddomesticuniversitiesto8. PhD programstaughtincollaboration These twoadditionsbroughtthenumberof agement of Zagrebinthefield with theFacultyofScienceUniversity larly, aPh.D.studyprogramincollaboration Importantly, 2003sawthesigningofan Figure 7. Figure 6. was launchedduringtheyear. Distribution ofthe RBIheldpostgraduatecourses Distribution oftheRBIheldpostgraduatecourses Molecular Medicine by domicileUniversity Environmental Man- by Division between the . Simi- our organizationremainsataskfor2004. formation intoanactualandintegralpartof tute's organizationalstructure)butitstrans- such aDepartment(aspartoftheInsti- This decisionsawtheformalcreationof Department ofEducationattheInstitute. to concludethatweshouldestablisha RBI ineducationledtheBoardofGovernors Science oftheUniversityZagreb). Physics Molecular andCellularBiology, Chemistry, Biochemistry, Oceanology, with theUniversityofOsijek), Nature andEnvironment The remainingprogramsare: The intensivelevelofinvolvementthe (in collaborationwiththeFacultyof (in collaboration Protection of and Organic Nuclear RBI annual report 2003. 7 larly noteworthy given nounced in the case of oceanology & chemistry, geology and physics where 7 to 9 of the 10 to most productive Croa- 11 tian projects were carried out at the RBI. However, excellence in publishing papers in CC journals at the RBI was also evident for the fields of biology (5/10) and biomedicine (4/10). This latter achievement is particu- Table 1 shows the number of (CC- 1 shows Table for the productivity assessment of either the for the productivity research group, it Institute or an individual the Insti- an important insight into does offer the sig- expect that We tute's achievement. and commercial nificance of educational is not reflected in the contributions, which is apparent from the publication numbers, appropriate sections of this contents of the report. by the sub- indexed) publications produced projects set of those fundamental research of publi- carried out at the RBI that, in terms top 10 proj- cations, were ranked among the research ects in Croatia in their respective accord- fields. It is clear from the table that, were ing to this criterion, the RBI projects fields of sci- among the most prominent in all This is especially pro- ence during 2003. . Publication output of the RBI since 1996 Figure 8

While the use of a simple (CC-based) Working with the benefit of new project with the benefit of new Working In the summer and autumn of 2002, 121 In the summer and RBI Bibliography Bibliography RBI publication count is not a complete criterion publication count is not a complete financing, scientists from the RBI published financing, scientists from the RBI indexed in 408 research articles in journals This is a Current Contents (CC) in 2003. productivity 39% increase on the average of pre- for the period 1996-2002 (the period 8. Similar vious project grants, see Figure for articles increases were also observed and books published in other journals (33%) and book chapters (21%). new projects from RBI received grants by new projects from Sev- Technology. & the Ministry of Science received grants for the eral research groups for others the new grants first time, while increase in the research represented an support. 8 RBI annual report 2003. previously notaccessible.Inaddition,2003 participate infieldsofresearchthatwere ment enabledscientistsattheInstituteto RBI duringthelastthreeyears. This equip- Ministry ofScienceand Technology tothe ty ofresearchequipmentgrantedbythe increase wascertainlythesignificantquanti- comments. Onefactorcontributingtothis lications was,andthusdeservessomebrief 2003, thelargeincreaseinCCindexedpub- Croatian sciencewasnotanewfactorin tive fields. RBI rankamongthetop20intheirrespec- terion manyotherprojectscarriedoutatthe be mentionedthataccordingtothesamecri- within thetop10Croatianprojects,itshould tabulated presenceofprojectsfromtheRBI this fieldbytheMinistry. Inadditiontothe the sheernumberofprojects(399)fundedin Although theprominenceofRBIin TABLE 1 top 10Croatianprojectsin2003 intheirrespectivefieldsofscience.Thelistwasprepared according toNationalBibliography database-onlyresearcharticlespublishedinjournals (next page):Publicationresults ofRBIfundamentalresearchprojectsrankedamong placed oncommercialandappliedsciences. in fundamentalresearch,withlessemphasis ties iscurrentlyorientedtowardsexcellence taken toindicatethatthefocusofouractivi- RBI. Concomitantly, thedata canalsobe Table 1,confirmthehighreputationof average (4). This data,combinedwiththatin a slightincreasefromtheanalogousannual patent applicationsin2003(6)represented per year). At thesametime,numberof annual averagefor1996-2002(46lectures entific lecturesin2003,almosttwicethe put. cant factorintheincreasedpublicationout- CC journals,thiswasquitelikelyasignifi- marily onthenumberofarticlespublishedin criteria fortheseprocedureswerebasedpri- for about130scientistsattheRBI. As the was ayearofpromotionsandre-elections Our expertswereinvitedtogive85sci- indexed inCurrentsContents werecounted.

Theoretical Physics ZTF Division

http://thphys.irb.hr

DIVISIONAL ORGANISATION 2003, the members of the Division contin- Head: Branko Guberina ued to be involved in lecturing at the University of Zagreb and a number of stu- Theoretical Physics Division (ZTF) dents completed their B. Sc., M. Sc. and consists of the following laboratories: Ph. D. theses.

DSolid State Physics Group, TOP ACHIEVEMENTS Radovan Brako

DParticle Physics and Cosmology Quantum well states in nanometre films Group, Neven Biliæ The understanding of nanometre thick DTheoretical and Mathematical metal films on metal surfaces is of funda- Physics Group, Stjepan Meljanac mental interest in terms of the discovery DGroup for Linear and Nonlinear and characterization of new structural and Dynamics, Mladen Martinis electronic properties. The formation of dis- crete electronic states, so-called quantum OVERVIEW OF THE DIVISION well states is one such intriguing feature. In this respect, our ab initio numerical calcu- The research performed in the Division lations found structural parameters of 1-5 is mainly concerned with the theoretical monolayer silver films in good agreement investigation of “high-energy” physics, with photoemission and STM measure- such as particle physics, general and ments obtained by collaborating experi- B nulrpr 2003. report annual RBI mathematical physics, astroparticle mental groups (M. Kralj et al., Phys. Rev. B physics and cosmology. In addition, there 2003, 68, 195402). exists a healthy level of investigation into problems in condensed matter physics. A Superluminal pions found relatively new and exciting activity in the In a recent study of the propagation of Division is the application of nonlinear pions at finite temperature in a hadronic dynamical analysis in biomedicine. In fluid, it was found that the velocity of mass-

11 12 RBI annual report 2003. ZTF Phys. J.C mentally discovered(W. Behr which noncommutativitycouldbeexperi- tive SM,representapotentialmeansby from thegaugesectorofNoncommuta- that twoparticulardecaymodes,coming boson interactionsareallowed.We propose however, suchSM-forbidden triplegauge bosons. Onnon-commutativespacetime, strictly forbidscertaindecaymodesofZ observation ofnoncommutativity Potential fortheexperimental for cosmologicalparameters,wedemon- model. Startingfromthepresenteravalues incorporated intothestandardcosmological cosmological constantwasconsistently Fate oftheUniverse D than light(N.BiliæandH.Nikoliæ, under certainconditions,propagatefaster pions inthechiralsymmetrybrokenphase, tantly, one-loopcalculationsshowedthat less pionsdiffers fromthatoflight.Impor- 2003 Figure 1. The StandardModel(SM)ofspacetime The renormalizationgrouprunningofthe , 68 Seminar room:juniorresearchers andPh.D.students 2003 , 085008). , 29, 441). et al. Phys. Rev. , Eur. Immun. progenitor cells(B.Vitale ronment insupportingcommonlymphoid tional restrictionsofthethymicmicroenvi- of age-dependent,geneticallyrelatedfunc- occursasaconsequence conclude thatCLL Leukaemia (CCL) Modeling ChronicLymphocytic 2003 Jonke andS.Meljanac, monic oscillatorandtheLandauproblem(L. applied ittoboththetwodimensionalhar- tion oftheutilityapproachwehave noncommutative spaces. As ademonstra- representations ofquantummechanicson of QMonnoncommutativespace Unified approachtotherepresentations Phys. Rev. D with criticalstringtheory(B.Guberina ingly, therunningalsorenderscompatibility changing thefateofuniverse.Interest- negative cosmologicalconstantthereby strate howtherunningcaneasilyprovidea As aresultofextensivemodelling,we We presentedaunifiedapproachtothe , 29 2003, , 433). .K.Passek-Kumerièki,“Hard 2. B.Meliæ,“LCSRanalysisof 1. SELECTED INVITEDLECTURES 2003 58 14.9.2003. Meeting, Dubrovnik,Croatia, 4- talk givenatthe9 order QCDcorrections”,invited exclusive processesandhigher- Taiwan, 29.9.-1.10.2003. violation(BCP3), Taipei, and CP tional Workshop onBphysics invited plenarytalkat3 exclusive two-bodyBdecays”, , 588). 67,083001). , et al. Eur. Phys.J.C th , Adriatic Scan. J.of rd Interna et al. , ZTF RBI annual report 2003. 13 , , gg 68 , , 2003 γγ D 2003 , 085008. 68 Phys. Rev. D Phys. Rev. Phys. Rev. D Phys. Rev. , 2003 Phys. Rev. B Phys. Rev. 441. Phys. Rev. B Phys. Rev. 29, , 2003 Phys. Rev. D Phys. Rev. , 68, 014013. , 67, 083001. tive project “Nanosciences: A “Nanosciences: tive project towards way 2003. December MZT, new technologies”, of M. Milun, Institute Dr. Project leader: Zagreb. Physics, Theoret- Institute, beetwen Ruðer Boškoviæ Theo- and Institut für ical Physics Division DFG Bochum, Germany, retische Physik II, QCD corrections in project “Higher-order and baryons”. exclusive processes-mesons of molecules in supramol- and organization macromolecules, and ecules, complexes, Coordinator: N. supermolecular structures. and I. Jalšenjak, Fac- Filipoviæ-Vincekoviæ, Bio- ulty of Pharmacy and Pharmaceutical Zagreb. chemistry, SELECTED PUBLICATIONS SELECTED PUBLICATIONS , 195402. 68 Laziæ, D. Šokèeviæ, R. Brako, Ž. Crljen, P. surfaces “Relaxation and reconstruction on (111) Au, Pt, and Cu” of 195411. pions in a N. Biliæ, H. Nikoliæ, “Superluminal hadronic fluid”, B. Meliæ, D. Müller, K. Passek-Kumerièki, “Next- B. Meliæ, D. Müller, to-next-to-leading order prediction for the photon-to-pion transition form factor” 2003 N.G. Deshpande, G. Duplanèiæ, P. Behr, W. “The Z J. Wess, Trampetiæ, Schupp, J. Collaborative projects Collaborative 1. in collabora- Brako: Participation Radovan 2. collaboration Kornelija Passek-Kumerièki, 3. M. Martinis: Research in self organization Laziæ, Ž. Crljen, M. Milun, P. Pervan, M. Kralj, P. A. Rosenhahn, K. Wan- R. Brako, J. Schneider, silver films on V(100):delt, “Tetragonal Experi- mental and ab initio studies” decays in the non-commutative standard model” Phys. J. C Eur. B. Guberina, R. Horvat, H. Štefanèiæ, “Renormal- ization-group running of the cosmological con- stant and the fate of the universe” 2003 pi pi D Meeting, IPPP nd pi pi” , invited talk given at Internation- talk given , invited pi pi” Adriatic Meeting, J. Trampetiæ, J. Wess Trampetiæ, Adriatic Meeting, J. D th linear systems, Mladen Martinis spaces, and symmetries, Stjepan Meljanac particle physics and cosmology, Branko Guberina strongly correlated systems, Radovan Brako from QCD light-cone sum rules”, invited from QCD light-cone sum rules”, 2 talk, CKM Workshop, mutative Gauge Theory”, invited lecture at Theory”, mutative Gauge 2003, WESS DAYS the workshop JULIUS From Mathematics to Noncommutativity: 2- Bayrischzell, Germany, Phenomenology, 5.5.2003. decays from QCD light-cone sum rules”, QCD light-cone decays from at the International con- invited plenary talk & CPference Flavor Physics Violation, FPCPParis, 2003, Ecole Polytechnique, France, 3-6.6.2003. al Europhysics Conference on High Energy Conference al Europhysics 17- Aachen, Germany, EPS, Physics 23.7.2003. B Durham, United Kingdom, 5-9.4.2003. Durham, United Kingdom, 5-9.4.2003. Adriatic Meeting (Central European Sym- Adriatic Meeting (Central European DIVISION PROJECTS INTERNATIONAL CONFERENCES ORGANIZED BY SCIENTISTS OF THE th 4. Structure of dynamical fluctuations in non- 3. 3. noncommutative Quantum field theory, 2. 2. Fundamental interactions in elementary 1. Physics of surfaces, microstructures and Projects supported by the Ministry of (MZT) Science and Technology (Eds.) 6. B. Meliæ, “Charming penguins in B 5. of Noncom- “Phenomenology Trampetiæ, J. 4. B of charmless Meliæ, “Some aspects B. 3. in Penguins of Charming “Estimate B. Meliæ, posia) – Particle Physics and the Universe, posia) – Particle Physics and the Chairmen: Dubrovnik, Croatia, 4-14.9.2003., Julius Wess. Trampetiæ, Josip Particle See also: “Lecture Notes in Physics: of Physics in the New Millennium”, Proceedings the 8 9 L. Jonke, S. Meljanac, “Representations of non- M. Dimitrijeviæ, L. Jonke, L. Möller, E. Tsouchni- commutative quantum mechanics and symme- ka, J. Wess, M. Wohlgenannt, “Deformed field tries” Eur. Phys. J. C 2003, 29, 433. theory on κ-spacetime” Eur. Phys. J. C 2003, 31, 129. S. Meljanac, M. Milekoviæ, A. Samsarov, “A mul- ZTF tispecies Calogero model” Phys Lett. B 2003, B. Vitale, M. Martinis, M. Antica, B. Kušiæ, S. 573, 202. Rabatiæ, A. Gagro, R. Kušec, B. Jakšiæ, “Prole- gomenon for Chronic Lymphocytic Leukaemia” Scan. J. Immun. 2003, 58, 588. RBI annual report 2003.

14 Division of ZEF Experimental Physics

http://www.irb.hr/str/zef

DIVISIONAL ORGANISATION OVERVIEW OF THE DIVISION IN 2003 Head: Ante Ljubièiæ until April 16th Acting Head: Roman Èaplar from April 17th The research goal of the Division is to obtain new basic knowledge of the structure The Division of Experimental Physics and behaviour of matter on the subatomic (ZEF) consists of the following level. In particular, we investigate elemen- laboratories: tary particles and their interactions, symmetries in nature, nuclear reactions, the struc- DLaboratory for nuclear reactions, ture of nuclei, the behaviour of nuclear mat- Ðuro Miljaniæ ter under extreme conditions, and the interaction of charged particles with matter. We DLaboratory for heavy-ion physics, Roman Èaplar develop and use new experimental techniques for measurements and applications. DLaboratory for electromagnetic We are also involved in teaching at the grad- and weak interactions, Ante Ljubièiæ uate and postgraduate levels and, as has been traditionally the case, we maintain an DLaboratory for ion-beam interactions, intense cooperation with world-leading cen- Milko Jakšiæ tres in nuclear and particle physics. DLaboratory for measurement of lowlevel activities, TOP ACHIEVEMENTS Bogomil Obeliæ

DLaboratory for high-energy physics, IRB Group Finds Evidence for More 2003. report annual RBI Krešo Kadija Pentaquark States in CERN Experiment DGroup for the development and the The constituent quark model of hadrons use of analytical methods, Vladivoj Valkoviæ has been very successful in describing the known baryons as composites of three valence quarks. The theory of strong interactions, however, does not forbid baryons

15 16 RBI annual report 2003. ZEF CERN Courier et al. fewer thanfivequarks(T. Antièiæ strangeness thatcannotberealisedwith baryon hasacombinationofchargeand accumulated overseveralyears. The new Boškoviæ whoanalysedrelatedNA49data a groupofscientistsfromtheInstituteRuðer NA49 collaborationatCERN,wasledby ure 1). This discovery, madebythe hypothesized baryonantidecuplet(Fig- confirmation oftheexistence important steptowardstheexperimental dicted pentaquarkstates,representsan candidates fortheisospinquartetofpre- discovery oftheexoticbarions,good had beenexperimentallyobserved. The but, untilrecently, nogoodcandidates states wereproposedalongtimeago with morethanthreequarks.Such commenced in2003with thedeliveryofa Upgrade oftheIRB Accelerator Facility baryons. Thestatesatthecornersoftriangleare Figure 1. quarks. Experimentsnowseecandidatesforthe manifestly exoticandcannotbemadeofthree The upgradeoftheaccelerator facility , Phys. Rev. Lett. hypothetical antidecupletofpentaquark A three circledmembers. , 2003 , 43, in press). 5; C. Alt, T.C. Alt, 5; Antièiæ et al. , important time anditsrelevancetotheastrophysically cussed (N.Soiæ the trum. Usingdatacollected in2003,oursci- axions associatedwitha broadenergyspec- experiment, designedto searchforsolar CERN weareinvolvedintheCAST mystery ofdarkmatterintheUniverse. At Quinn mechanism,couldalsoexplainthe in quantumchromodynamicsbythePeccei- problem in modelsresolvingthestrongCP Axions, whicharepseudoscalarsthatarise The searchfordarkmatter 63 Astrophysics Nuclear PhysicsMeasurementsand (Figure 2). extension oftheexistingacceleratorhall the newexperimentalareainnorthern accelerator willbeinstalledduring2004in This (IAEA). Energy Agency tional Atomic mainly bythejointprojectwithInterna- new 1.0MV Tandetron accelerator, financed Figure 2. 524) , The 12 B nucleuswasmeasuredforthefirst α aoto h E acceleratorfacility Layout oftheZEF + 8 8 Li decayoftheexcitedstates Li+ α et al. D 11 B+n reactionwasdis- , Europhys. Lett. , 2003 , ZEF RBI annual report 2003. 17 probe experimental equipment. Small (above) microchannel plates based New equipment was installed in the labo- In 2003, ZEF joined the major worldwide ZEF joined the In 2003, detector and medium size (below) nuclear micro- detector and medium size (below) nuclear Figure 4. Neutrons for applications ratory housing the refurbished neutron gen- Compressed Baryonic Matter Baryonic Compressed namely the inter- physics project, heavy-ion Baryonic Matter Compressed national goal of the collab- The (CBM) collaboration. and build a dedicated oration is to design to investigate the proper- detector in order baryonic matter ties of highly compressed in ultrarelativistic as it will be produced collisions at the Future nucleus-nucleus Facility at the GSI, Accelerator International Darmstadt. , Nucl. Instr. and Meth. B. Nucl. Instr. ., . This limit is approximately a This limit is approximately . -1 et al 237). at an intermediate energy. heavy-ion collision dynamics heavy-ion collision dynamics , GeV An example of semi-classical simulation of An example of semi-classical -10 , 210 A Resolved Ion Time the new version of Figure 3. entists were able to establish a new upper entists were able of bound on axion-photon coupling 1.4x10 Beam Induced Charge (TRIBIC) technique Beam Induced Charge (TRIBIC) was applied to study the temperature dependence of the shape of a transient pulse of charge, for the first time in 2003. This technique can provide information on the charge trapping mechanisms which limit the performance of such radiation detectors (Z. Meduniæ New experimental method factor of 4 more stringent than the best pre- factor of 4 more stringent than the proposed a vious estimate. In addition, we near- solar, novel approach to search for that could monochromatic hadronic axions, in an be created in the solar core. Finally, an upper experiment performed in 2003, mass was limit of 5.5 keV on hadronic axion obtained. 2003 18 RBI annual report 2003. ZEF lation patternsandclimate”. Mediterranean Basinin relation toaircircu- “Isotopic compositionof precipitationinthe projectentitled the three-yearlongIAEA the Adriatic regionwerecompletedwithin were continuedwithinthe5 investigations Radioactivity andecological projects. IAEA materials, issupportedbyEU,NATO, and trafficking ofnuclearandotherradioactive cal measurestodetectandrespondillicit also devotedtotheimprovementoftechni- vigorously continued. This work,whichis sives anddangerousmaterialshasbeen detection andidentificationofhiddenexplo- nuclear instrumentationandmethodsforthe erator. Development andapplicationsof ments of tia andBosnia-Herzegovina”.Measure- the BihaæregionatborderareaofCroa- the protectionofPlitviceNationalParkand war andtheestablishmentofmeasuresfor “Study ofanthropogenicpollutionafterthe Programme oftheEUonprojectentitled Figure 5. Ecological investigationsinDinaricKarst produced intritiumfusionhasbecomeknownastheneutrongenerator. Itisusedinappliedresearch. 2 The recentlyrefurbishedlow-energyacceleratorwhichdeliversenergeticneutronsviathedeuteron H, 3 H, and 18 O inprecipitationof th Framework .V. Valkoviæ (D.Sudac,S.Blagus,D.Matika, 6. N.Soiæ(N.Soiæ,W.N. Catford,S.Cherubi- 5. D.Mekteroviæ(D.andI.Supek 4. D.Kekez, 3. .K.Kadija, 2. .Z.Basrak, 1. CONFERENCES INVITED LECTURES AT INTERNATIONAL Brussels, Belgium. and NeutralisationofLandmines andUXO, International Conferenceon Requirements 14 MeVneutronsinexplosivedetection R. Kollar, T. Grivièiæ,V. Valkoviæ), lar structuresinlightnuclei Zadro), Romano, C.Spitaleri,D.L.Watson, M. Fulton, M.Lattuada,Ð.Miljaniæ,S. ni, N.M.Clarke,L.Donadille,M.Freer, B.R. 105 MeV/cand180 reaction inamomentumregionbetween cross sectionsofthechargeexchange for CrystalBallCollaboration), Jlab, Dubrovnik,Croatia, Nuclear andParticlePhysicswithCEBAFat hadrons and winger-Dyson approachtoQCD shop Jefferson Lab,NewportNews,USA, nances atNA49 Varenna, Italy, ence onNuclearReactionMechanisms, ativistic energies Experimental evidenceformolecu- , 2 oeof(A2)condensateinSch- Role Search forexoticbaryonreso- nd Nuclear opacityfromlowtorel- International Conferenceon , Penta-Quark2003Work- , 10 th International Confer- , ibid. , ibid. Differential The useof , ZEF RBI annual report 2003. 19 Framework Programme, (Bogomil th tron accelerator, Milko Jakšiæ (MZT, Infor- Milko Jakšiæ (MZT, tron accelerator, Project) mation Technology the database, for access to passwords Mario Stipèeviæ (MZTTechnolo- Information gy Project) coastal region of Croatia atmosphere at the Austria, IAEA, Vienna, (Nada Horvatinèiæ), in Environmental and Indus- Analysis Beam Jakšiæ and Bogomil Applications (Milko trial Austria, Obeliæ), IAEA, Vienna, (Bogomil Nuclear Analytical Techniques Austria, Obeliæ), IAEA, Vienna, mine and UXO detection and identification Austria, IAEA, Vienna, (Vladivoj Valkoviæ), for pro- war and establishing the measures Bihaæ tection of Plitvice National Park and and region at the border area of Croatia Commi- Bosnia-Herzegovina, European ssion, 5 Obeliæ), Atmosphere at the Coastal Region of the Austria (Nada Hor- Croatia, IAEA, Vienna, vatinèiæ), oxygen and hydrogen in precipitation in ecological and hydrological investigations (Ines Krajcar Broniæ), Croatian-Slovenian joint research project, Karst (Bogomil Obeliæ), Croatian-Slovenian joint research project, tems of National Park Plitvice to the quality of water and lakes (Nada Horvatinèiæ), National Park Plitvice, Croatia, Research and development projects development and Research 1.Tande- of 1.0 MV aided control Computer 2. user with individual Secure web server Other projects 1. the and stable isotope distribution in Tritium 2. and Ion Techniques Nuclear Spectroscopy 3. Control of Assurance/Quality Quality 4. Facility for testing nuclear methods for land- 5. Study of anthropogenic pollution after the 6. Distribution in and Stable Isotope Tritium 7. Determination of isotopic composition of 8. Monitoring of anthropogenic influence in 9. Investigation of influence of forest ecosys- Symposium of the Croatian Radiation of the Croatian Symposium th namics, Tomislav Iveziæ namics, Tomislav material detection, Vladivoj Valkoviæ material detection, Vladivoj Valkoviæ Krešo Kadija velopment of instrumentation, Bogomil Obeliæ ter, Milko Jakšiæ ter, Tihomir Suriæ Suriæ Tihomir Ante Ljubièiæ physics, Alfred Švarc cules, reactions, Ðuro Miljaniæ anthropogenic pollution after the war and anthropogenic pollution for protection of establishing the measures and Bihaæ region at Plitvice National Park of Croatia and Bosnia- the border area Park Plitvice Lakes, Herzegovina, National Orga- Croatia (B.Obeliæ - chairman of the nizing Committee). Protection Association, Protection Association, Stubièke toplice, - chairman of the Orga- Croatia (B.Obeliæ I. Krajcar-Broniæ - chair- nizing Committee, Committee) woman of the Scientific Nuclear And Particle Physics (NAPP), Particle Physics And Nuclear - co-chairman) Croatia (I. Supek Dubrovnik, CONFERENCES ORGANIZED BY ORGANIZED CONFERENCES DIVISION OF THE SCIENTISTS PROJECTS 11. Invariant special relativity and electrody- 10. Methods of explosive, chemical and nuclear 9. High-energy experimental physics, 8. Natural isotopes of weak activities and de- 7. Processes of fast ion interactions with mat- 6. Photon-atom interactions and correlations, 4.5. Heavy-ion physics, Zoran Basrak Massive neutrinos and astro-particles, 3. Interactions in subatomic and medical 1.2. Hadronic physics and QCD, Ivan Supek Light atomic nuclei: clusters, nuclear mole- Projects supported by the Ministry of Projects supported by the Ministry (MZT) Science and Technology 3. of the EU project Study of meeting Kick-off 2. 5 1.on Conference International Second 10. Control of illicit trafficking in explosives, N.Soiæ, S.Cherubini, M.Lattuada, D.Miljaniæ, S. chemical agents, and humans (Vladivoj Romano, C.Spitaleri and M.Zadro. 8Li+α decay Valkoviæ), IAEA, Vienna, Austria, of 12B and its astrophysical implications”, Euro- 11. Inspection of shipping containers for undis- phys. Lett., 2003, 63, 524-530.

ZEF closed radioactive materials (Vladivoj Z. Meduniæ, M. Jakšiæ and Ž. Pastuoviæ. Temper- Valkoviæ), IAEA, Vienna, Austria. ature dependent TRIBIC in CZT detectors, Nucl. Instr. and Meth. B., 2003, 210, 237-242 SELECTED PUBLICATIONS A. Androniæ et al. Directed flow in Au + Au, Xe + T. Antièiæ, K. Kadija, T. Šuša and P. Seyboth. CsI, and Ni + Ni collisions and the nuclear equa- New five-quark states found at CERN, CERN tion of state, Phys. Rev. C, 2003, 67, 034907 Courier, Dec. 2003, vol. 43, No. 10, p. 5. Z. Basrak, R. Èaplar and V. Paar (guest editors). C. Alt, T. Antièiæ et al. Evidence for an Exotic S = Fizika B, 2003, 12, 1-192. –2, Q = –2 Baryon Resonance in Proton-Proton Collisions at the CERN SPS, Phys. Rev. Lett., in N. Soiæ et al. Cluster structure of 13C probed via press 7Li(9Be,13C* D 9Be*α) reaction, Nucl. Phys. A, 2003, 728, 12. C. Alt et al. Directed and elliptic flow of charged pions and protons in Pb + Pb collisions at 40A and 158A GeV, Phys. Rev. C, 2003, 68, 034903 RBI annual report 2003.

20 Division of Materials ZFM Physics

http://www.irb.hr/str/zfm

and macroscopic properties of materials, as DEPARTMENT ORGANISATION well as nanophase and glassy material char- Head: Branko Pivac acteristics, prove particularly important. The methods of investigation include the study of The Division of Materials Physics (ZFM) amorphous thin films produced by non-ther- consists of the following laboratories: modynamic processes (magnetron sputtering), particularly Al-W and WC, a-Si and a- D Laboratory for semiconductors, Branko Pivac Si1-xCx:H films. In addition, the study of processes in magnetron sputtering sources D Laboratory for thin films, Nikola Radiæ and plasma obtained during laser ablation of D Laboratory for molecular physics, metallic and non-metallic materials, is Krešimir Furiæ undertaken. The ZFM is also involved in fundamental research in the field of molecular and solid state physics with special empha- OVERVIEW OF THE DIVISION sis on vibrational spectroscopy. The systems under investigation vary in their origin The Division of Materials Physics is and composition from metals, semiconduc- focused mainly on fundamental and applied tors, and ceramics on one side, to molecular studies of physical parameters and process- crystals and biological samples on the other. es that describe and connect the microscopic and macroscopic properties of condensed TOP ACHIEVEMENTS matter and molecules. The main goal of the B nulrpr 2003. report annual RBI ZFM is to obtain a better understanding of Nanosciences the fundamental characteristics of defects in Nanoscience and/or nanotechnology rep- simple (Si), binary (GaAs, GaN, CdS), and resent the most promising direction for both multinary semiconductors and their comple- modern fundamental scientific research and mentary oxides. Certain aspects, such as developments in technology. In 2003, the the mutual interactions of defects, their Croatian Nanonetwork was established, with impact on the relation between microscopic the aim of stimulating collaboration amongst

21 22 RBI annual report 2003. ZFM peratures upto800 and theheatingassemblycanhandletem- er isprovidedwithDCorRFbiascapability, movablesubstratehold- tors, orisolators. A get materials-metals,alloys,semiconduc- deposition ofuptothesamenumbertar- nished withshutters,allowco-orsequential tions. Fourmagnetronsputteringgunsfur- al targetsundervariousdepositioncondi- thin filmsbymagnetronsputteringofsever- preparation ofnewmaterialsintheform USA, thesystemisdesignedtoallow and deliveredbytheKurtJ.Lesker, Co. the DivisionofMaterialsPhysics.Produced installed intheLaboratoryfor Thin Filmsof The CMS-18sputteringsystem Pivac, N.Radiæ, A. Turkoviæ andD.Gracin. From 22domesticprojects,4areledbyB. and 15formal26internationalprojects. tute ofPhysics)andinvolves7institutions nologies" iscoordinatedbyM.Milun(Insti- entitled "Nanosciences:aroadtonewtech- with significantfuturepotential. The project, tified severalpromisingnucleationcenters research. Indeed,thefirstresultshaveiden- on afewselectedprincipaldirectionsof tacts andfocustheefforts ofdifferent groups a collaborationwillencourageclosercon- ment suitableforresearchinthisfield.Such different groupshavingexpertiseandequip- puter-controlled. tion processmaybemanually orfullycom- gases allowforrectivedeposition.Deposi- working gas(argon),twoinletsforreactive for LN2coolingisalsoavailable.Besides The CMS-18sputteringsystemhasbeen o C. A separate assembly separate C. A Forum icon (B.Pivac determination oftheinterstitialoxygeninsil- with significantlyimprovedaccuracy, forIR ponent, thearticlepresentedanewmethod, est. Inadditontotheliteraturereviewcom- ogy, thiscontributionisofwidespreadinter- important inmodernsemiconductortechnol- lenged. As thisparameterisextremely determination ofoxygeninsiliconwerechal- standing DINand ASTM standardsforIR al. was basedontheirrecentpaper, B.Pivac content insilicon New standardsinmeasuringoxygen In responsetoaneditorialinvitation,that published areviewarticleinwhichlong Figure 1. , 2003 , 221-223,123). The CMS-18sputteringsystem et al ., Defect andDiffusion et ZFM RBI annual report 2003. 23 2 GISAXS patterns suggest that the syn- GISAXS patterns suggest that substrate by ion implantation and annealing, substrate by ion implantation and was established. Possible benefits of a novel method may not be thesis of nanoparticles (which amorphous) yet fully ordered, possibly even If, occurs already during ion implantation. during indeed, II-VI QDs are already formed of high-dose implantation, the production would high-quality QDs of II-VI compounds of already be reduced to re-crystallization diffu- formed compounds, without requiring In this sion of the compound components. case, much lower annealing temperatures which is desirable since would be sufficient thermally driven, equilibrium, thermodynamic processes tend to broaden the size distribution of QDs. (QDs) of compound semiconductors (CdS, (QDs) of compound semiconductors embedded in SiO and PbTe) CdTe ZnTe, Executive Council Meeting of IUVISTA, Dubrovnik of IUVISTA, Executive Council Meeting rd The 93 Figure 2. Non Thermodynamic Executive Council Meeting of production of quantum dots rd Figure 3. The 93 The viability of the synthesis of a 3D The viability of the synthesis International conference ensemble of well crystallized quantum dots ensemble of well crystallized quantum Non thermodynamic production of Non thermodynamic production quantum dots IUVSTA, Dubrovnik, 19-21 September 2003 Dubrovnik, IUVSTA, N. was organized by the division members Radiæ and B. Pivac. PROJECTS SELECTED PUBLICATIONS

Projects supported by the Ministry of B. Pivac, A. Sassella, and A. Borghesi. Recent Science and Technology (MZT) “Advances in the Measurement of Interstitial

ZFM Oxygen in Silicon by Infra-Red Spectroscopy”. 1. Impact of Defects and Nanostructures on Defect and Diffusion Forum, 2003, 221-223, 123- Semiconductor Properties. B. Pivac 131. 2. Magnetron Deposition of Thin Films. I. Desnica-Frankoviæ, U. Desnica, P. Dubèek, M. N. Radiæ Buljan, S. Bernstorff, K. Helmut, B. Stritzker, “Ion 3. Physics and Application of Nanostructures. beam synthesis of buried Zn-VI quantum dots in K. Furiæ SiO : GISAXS studies“. Journal of Applied Crys- 4. Multiphase Amorphous Silicon Alloys as 2 talography, 2003, 36, 439-442. Thin Films. D. Gracin 5. Sugar Hydratation Dynamics. U. Desnica, P. Dubèek, I. Desnica-Frankoviæ, M. V. Mohaèek-Grošev Buljan, S. Bernstorff, C.W. White, “GISAXS stud- 6. Nanophase Films and Nanocomposite Solid ies of the synthesis and growth of CdS quantum

Electrolytes Research. A. Turkoviæ dots from constituent atoms in SiO2 matrix“. 7. Structure and Electrical Relaxation in Journal of Applied Crystalography, 2003, 36, Glasses and Glass-Ceramics. 443-446. A. Moguš-Milankoviæ 8. Statics and Dynamics of Molecular Solids. P. Dubèek, B. Pivac, S. Bernstorff, F. Corni, R. D. Kirin Tonini, G. Ottaviani, “Grazing incidence small- 9. Optical Interactions and Organizational angle X-ray scattering study of defects in deu- Processes in Matter. S. Lugomer terium implanted monocrystalline silicon“. Jour- 10. Semiconductor Materials for nal of Applied Crystalography, 2003, 36, 447- Optoelectronics. B. Šantiæ 449. V. Desnica, K. Furic, B. Hochleitner, M. Mantler, Research and development projects “A comparative analysis of five chrome green pigments based on different spectroscopic tech- 1. Reseach collaboration contract with the fac- niques“. Spectrochimica Acta Part B. 2003, 58, tory “Solar cells”, Split (D. Gracin) 681– 687. 2. Stress in thin films (N. Radiæ), (Bilateral collaboration with Slovenia) M. Ivanda, K. Babocsi, C. Dem, M. Schmitt, M. 3. Study of nanocomposite polymer electro- Montagna, W. Kiefer, “Low-wave-number Raman lites (A. Turkoviæ), (Bilateral collaboration scattering from CdSxSe1-x quantum dots with Slovenia) embedded in a glass matrix“. Physical Reveiw B. 4. Study of disordered materials, nano-optical 2003, 67, 235329-235338. layers (M. Ivanda), (Bilateral collaboration with Slovenia) 5. Chemically durable iron phosphate glasses for vitrifying stimulated nuclear waste (A. Moguš-Milankoviæ), (IAEA) RBI annual report 2003.

24 Division of Laser and

Atomic Research and LAIR Development

http://www.irb.hr/str/lair

several types of malignant diseases, the first DIVISIONAL ORGANISATION results of which are very promising. Head: Hrvoje Zorc LAIR is also involved in basic research and development, as well as the small scale The Division of Laser and Atomic production of optical instruments and aiming Research and Development (LAIR) devices for defence systems. The Division consists of the following laboratories: also provides user oriented educational D Laboratory for Optics and Thin Films, courses for maintenance and service of the Hrvoje Zorc aforementioned optical devices. D Multipurpose workshops, Eduard Švegel TOP ACHIEVEMENTS ¨ OVERVIEW OF THE DIVISION The major achievement in 2003 was the development of an advanced, computer The activities of the division have been controlled, apparatus for photodynamic directed towards the development of new diagnosis and therapy under the commercial medical instrumentation, particularly in the name "MediLED 4 PDD & PDT" (patent field of photodynamic therapy using novel pending). This comprehensive system incor- advanced light sources such as light emit- porates highly efficient light sources - light ting diode clusters. Research and develop- emitting diode clusters and is designed for ment in the fields of optical design, electron- use in clinical practice. B nulrpr 2003. report annual RBI ics, and mechanical design, combined with a permanent interaction with the University Simultaneously, the MediLED Vio and clinic, resulted in several new devices for MediLED Thera devices were produced as photodynamic therapy. Such devices are portable, easy to use light sources for treat- applicable to certain skin tumours, pre- ment of smaller lesions (patent pending). malignant diseases and other dermatoses. MediLED devices were exhibited for the first In addition, clinical research has started on time during the 5th International Symposium

25 SELECTED PUBLICATIONS

Radman, I., Peršin, A., Zorc, H., Tisaj, K., Stanišiæ, V., Lonèariæ, M., Soldo Roudnicky, D., Radman, A.: LAIR First results of the use of new LED- based light sources on PDD and PDT of Mycosis Fungoides, 5th International Symposium on Photo- Figure 1. MediLED 4, MediLED Vio and Thera dynamic Diagnosis and Therapy in Clinical Practice / B. Kramer, H. Kostron, G. Jori (ur.), Bressanone, on Photodynamic Diagnosis and Therapy in 2003. 31 Clinical Practice (Bressanone, Italy, 7-11 Harvey, J.E. Bogunoviæ, D., Krywonos, A.: Aber- Oct. 2003). rations of Diffracted Wave Fields: Distortion., Applied Optics - OT. 2003, 42, 1167-1174.

PROJECTS Janicki, V., Zorc, H.: Determination of refractive index profile of ZrO2 on amorphous and pre- Projects supported by the Ministry of Science evaporated substrates by reverse engineering, and Technology (MZT) Proc. SPIE, 2003, 5250, 546-53. Piegari, A., Janicki, V.:Metal/dielectric coatings 1. Photonics of image- and non-image optical for transmission filters with wide rejection bands, systems, Hrvoje Zorc Proc. SPIE, 2003, 5250, 343-48.

Research and development projects Pavloviæ, M., Šantiæ, B., Desnica-Frankoviæ, D.I., Radiæ, N., Šmuc T., Desnica, U.V.: The impact of 1. Light sources for photodynamic therapy of deep levels on the photocurrent transients in tumors, Anton Peršin (HITRA-TEST) semi-insulating GaAs, J. of Electronic Mater. 2003, 32, 10. RBI annual report 2003.

Figure 2. Testing MediLED 4 in the laboratory

26 Division of Electronics ZEL

http://www.irb.hr/str/zel

department KU, Leuven, Belgium. Božidar DIVISIONAL ORGANISATION Vojnoviæ, the former head of the Division, Head: Tomislav Šmuc retired at the end of the year. The Division of Electronics (ZEL) TOP ACHIEVEMENTS consists of the following laboratories:

D Laboratory for information systems, Methodology for subgroup discovery Dragan Gamberger An original approach to subgroup discov- D Laboratory for stochastic signals and processes research, Ivan Michieli ery and representation was applied to problems related to the detection/classification of patients suffering from coronary heart disease. This approach has also been applied OVERVIEW OF THE DIVISION to marketing research problems, and has found use as an online data mining tool (B. In 2003, the Division of Electronics Cestnik et al., Data mining and decision sup- broadened the scope of its research with port, Kluwer Academic Publishers, 2003). applications and original contributions in the fields of bioinformatics (gene array expres- New areas of research sion data analysis, compaction and indexing New areas of interest within ZEL include DNA sequences) and time-series modelling. applications of machine learning methods to New international collaborations were initiat- gene array expression analysis and com- ed in the field of data mining, with the AI 2003. report annual RBI pression and indexing of large DNA Department at the Josef Stefan Institute (AI- sequence databases. Important contribu- IJS), Slovenia, and in material behaviour tions have also been made in the areas of modelling, with the Institute for New Materi- software engineering and modelling of als in Saarbruecken, Germany. The labora- chaotic signals and processes (A. Maksi- tory for information systems organized a moviæ et al., J. Fluid. Struct. 2003, 17, 317. joint conference ECML/PKDD-2003, in Cav- I. Michieli, B. Vojnoviæ, J. Comp. Inf. Tech- tat in collaboration with AI-IJS, and the CS nol. 2003, 11, 55).

27 28 RBI annual report 2003. ZEL Figure 1. Figure 2. PEH-UH:InformationPortalofepidemiologicalstudy IP International Standardsforthe flowMeasurements Arterial HypertensioninCroatia - Active NetworkObjects .Informationportal for theepi 2. 1. supported byMZT Information technologyprojects AnalysisofStochasticSignals, 2. 1. Automated Knowledge Technology (MZT) Ministry ofScienceand Basic projectssupportedbythe gram of thecurriculumPh.D.pro- (Dragan Gamberger)isnowpart discovery inmedicaldomains newcourseentitled A Educational activities Information technologyprojects. were realizedwithinthescopeof Science and Technology (MZT) financed bytheMinistryof New WEBportals the UniversityofZagreb. Sciences, PROJECTS Two newWEBportals, Tomislav Šmuc hypertension inCroatia, demiological studyofarterial N flow measurements- I Structures, BožidarVojnoviæ Time SeriesandData Nikola Bogunoviæ Discovery andReasoning, nternational etwork Biomedicine &Health at theMedicalSchoolof O bjects, IvanMariæ S tandards forthe Knowledge A ctive , ZEL RBI annual report 2003. 29 , 2003 , 497- Artificial 42 , , 1, 55-66. 11 , 2003 , 27-57. . 28 , 6-11. , 1 2003 , . Journal of Computing 2003 . 2003 . 2003 Journal on Systemics, Cyber- Journal on Systemics, ISA Transactions Data mining for decision support: Sup- for decision support: Data mining Journal of Fluids and Structures. SELECTED PUBLICATIONS SELECTED , 317-330. Cestnik, B., Lavraè, N., Flach, P., Gamberger, D., Gamberger, P., Lavraè, N., Flach, Cestnik, B., Kline, M. through subgroup decisions porting marketing support, mining and decision Data discovery, S. M., Moyle, D., Lavraè, N., Bohanec, Mladeniæ, Aca- Kluwer (editors). Boston/Dordrecht/London: demic Publishers, Bogunoviæ, N., Grudeniæ, I., Pek, E. A I., Pek, E. Bogunoviæ, N., Grudeniæ, Synthe- of Com- for Formal Verification sized Framework puting Systems, netics and Informatics Gamberger, D., Lavraè, N., Krstaèiæ, G. Active D., Lavraè, N., Krstaèiæ, G. Gamberger, A Subgroup Mining: in Coronary Case Study Group Detection, Heart Disease Risk Intelligence in Medicine. 504. B. Detecting Noise in Michieli, I., Vojnoviæ, Component Chaotic Signals through Principal Matrix Transformation, and Information Technology S., Michieli, I. Multi- A., Lugomer, Maksimoviæ, of axial solitons on vortex filaments: the origin tangling. 17 Mariæ, I. Software objects in distributed flow Mariæ, I. Software objects in distributed measurements, European Confer- th European Conference th rtment, Jozef Stefan Institute, Ljubljana, rtment, Jozef Stefan Slovenia environmental research on a project “Devel- research on environmental sensor the new electrochemical opment of of waterfor the measurement pollution” (LISSP) CONFERENCES Organization of the joint conference Organization of ECML/PKDD 2003 (14 2.AI Depa- Analysis with Intelligent Data 1. and for marine with Division Collaboration Research, development and collaborative and development Research, projects Machine Learning: ECML 2003, Lavraè, Nada; Todor- Dragan; Blockeel, Hendrik; Gamberger, 2003 ovski, Ljupèo (editors), Berlin: Springer, (conference proceedings). Knowledge Discovery in Databases: PKDD Knowledge Discovery in Databases: Dragan; Block- 2003, Lavraè, Nada; Gamberger, Ljupèo (editors). Berlin: Todorovski, eel, Hendrik; 2003, (conference proceedings) Springer, ence on Principles and Practice of Knowledge ence on Principles and Practice of 22.- Discovery in Databases), Cavtat, 26.09.2003. on Machine Learning and 7

Division of Physical ZFK Chemistry

http://www.irb.hr/str/zfk

contributions were published in the highest DIVISIONAL ORGANISATION ranking chemical journals such as the Jour- Head: Aleksandar Sabljiæ nal of the American Chemical Society, Chemistry - A European Journal, the Journal The Division of Physical Chemistry (ZFK) of Physical Chemistry, the Journal of Organ- consists of the following laboratories: ic Chemistry, and Biochimica et Biophysica D Laboratory for Chemical Kinetics and Acta. The contribution to undergraduate and Atmospheric Chemistry, Dunja Srziæ graduate education this year was extensive D Laboratory of Radiochemistry, and the division continued to participate in Nada Filipoviæ-Vincekoviæ the organization of highly regarded scientific conferences. In addition, the traditional inter- D Theoretical Chemistry Group, Tomislav Živkoviæ national collaborations with institutions such as Harvard University, Rice University, D Laboratory of Chemical and Biological Pacific Northwest Laboratory, Free Universi- Crystallography, Biserka Kojiæ-Prodiæ ty Berlin, and the Hebrew University of D Laboratory for Magnetic Resonances, Jerusalem were maintained. Boris Rakvin

D Laboratory for Analytical Chemistry, TOP ACHIEVEMENTS Ivan Habuš Probing the properties of molecular magnets OVERVIEW OF THE DIVISION

Single molecule magnets (SMMs) are the 2003. report annual RBI In 2003, the members of ZFK contributed focus of many experimental and theoretical valuable publications on a broad range of studies. They exhibit the novel phenomenon topics such as atmospheric chemistry, struc- of quantum tunnelling of the magnetization. tural chemistry, theoretical chemistry, the In the context of SMMs, a single molecule modelling of physical and chemical process- may exhibit a hysteresis loop and hence has es, structural and chemical analyses, and the potential to serve as the basis of memo- biosciences. A significant number of these ry storage at the molecular level, and possi-

31 32 RBI annual report 2003. ZFK related adamantyltripeptides, withmultil- peptidoglycan monomer andthestructurally potential immunostimulating agents,the was usedtostudythe interactionsofthe antigens andvaccines.EPRspectroscopy in stimulatingtheimmuneresponsetoweak gen. Suchcompoundsareparticularlyuseful the immuneresponseofhosttoananti- immunostimulants Potential newdeliverysystemfor potential molecularmagnets. properties ofhighlymagneticmolecules,i.e. and inexpensivescreeningtoolforprobing potential tobecomeanaccurate,efficient Res. net Mn field nearthesurfacesofmolecularmag- was developedtomeasurethemagnetic tive method,basedonEPRspectroscopy, sensi- bly evenforquantumcomputation. A Figure 1. Immunostimulating compoundsenhance to measuremagneticfieldsatthesurfaceof 2003 12 nanomagnet Mn Images ofEPRprobeNMP-TCNQutilized under magnificationat60times -acetate (B.Rakvin , 165 , 260). This methodhasthe 12 -acetate shown et al.J.Mag. in vivo(R.Frkanec dates foruseasimmunostimulatingagents charged liposomesmakesthemgoodcandi- adamantyltripeptides withthenegatively amellar liposomes. The stronginteractionof Acta is possibletomanipulate thearrangementof wormlike tosphericalmicelles. Inthisway, it modify thesupramolecular structurefrom presence ofalongerspacer wasfoundto amphotropic properties.Inparticular, the parameter fortheirstructuraland bic spacerinthesemoleculesisacritical It wasfoundthatthelengthofhydropho- factants, namelythedissymmetricgeminis. approach involvinganewgenerationofsur- tionships arestudiedbyaninterdisciplinary vesicles, liotropicliquidcrystals).Suchrela- supramolecular organization(intomicelles, tionships betweentheirstructureand ties, itisimperativetounderstandtherela- properties Designing surfactantswithdesirable To designsurfactantswithspecificquali- Figure 2. spectrometer (FT/CWEPR)workingwiththe 2003 pulse andcontinuousmicrowavesource in awidetemperaturerange(4K-300K) , 1611 Electron ParamagneticResonance , 187). ta.Biochim.Biophys. et al. ZFK RBI annual report 2003. 33 th nd ”, 8 12 and Mn 8 MATH/CHEM/COMP, Dubrovnik, 23-28 MATH/CHEM/COMP, th theoretical methods and applications” Col- applications” and methods theoretical projectlaborative the Ministry supported by Technology. and of Science Cam- University, magnetic fields” Harvard 27 January 2003 bridge, MA, U.S.A., of Ele- “The Dynamics Workshop NATO Reactions” Balatonföld- mentary Chemical 8-12 June 2003 Hungary, vár, and adamantyl- peptidoglycan monomer into liposomes” 2 tripeptides entrapped June 2003 2003 and Function”, Rovinj, 14-26 Sept. Cvitaš microconstituents, Tomislav interfaces, Nada Filipoviæ-Vincekoviæ Klasinc Aleksandar Sabljiæ ultrafast processes, Workshop ELECTRON MAGNETIC Workshop RESONANCE OF DISORDERED November SYSTEMS, Sofia, Bulgaria, 11 2003 International Conference on Biomedical London, UK, 5-8 July 2003 Spectroscopy, of measuring magnetic field at the surface single molecule magnets Fe SELECTED INVITED LECTURES SELECTED PROJECTS CONFERENCES ORGANIZED BY SCIENTISTS OF THE DIVISION 3. and experimental and ultracold “Ultrafast 1. in ultra strong electro- S. Bosanac “Charge 2. in phase space” S. Bosanac “Dynamics 3. “A Noethig-Laslo V. study of spin-labelling 2. Summer School “Supramolecular Structure Projects supported by the Ministry of (MZT) Science and Technology 1. Properties and behavior of atmosferic 2. Surfactants, processes in solution and at 3. Reactivity and reaction mechanism, Leo 4. Investigation on chemical reactivity and 1. 18 4. B. Rakvin “An EPR spin probe method for , 19 2003, J. Org. Chem. Langmuir . et al to the giant vesicular phase to the giant vesicular Transformation of dissymmetric gemini of dissymmetric Transformation , 5777). These results will help to , 5777). 68 surfactants from a fan shaped texture surfactants from a fan , tions”, Greta Pifat-Mrzljak and early evolution”, Vesna Noethig-Laslo and early evolution”, Vesna Award for Science and Art, I Order for Science and Award The electronic structures of the anti-HIV The electronic structures of the SELECTED COLLABORATIONS AWARDS Figure 3. 2. COST D-22 Project “Protein-lipid interac- 1. COST chemistry D-27 action “Origin of life 1. 1. Austrian Honorary Greta Pifat-Mrzljak: drug AZTdrug the antimalarial drug artemisi- and spec- nine were resolved by photoelectron calcula- troscopy and quantum-chemical tions (I. Novak, B. Kovaè, surfactants in micellar, mesomorphic and surfactants in micellar, to and, consequently, crystalline states character- design surfactants with desirable istics (M. Sikiriæ drug Electronic structure of anti-HIV AZT revealed 10044). 2003 biological clarify the mechanisms of their anti- activity as well as to derive improved HIV and antimalarial agents. 34 RBI annual report 2003. ZFK .Structuralinvestigationofnaturalpolymers 6. Interactionofbiomembraneswithpeptides, 5. Structureanddynamicsofinterpenetrating 4. MolecularBasisforBiocatalysisbyMicro- 3. PredictionofEnzymeSelectivity, Regula- 2. Lipoprotein interactionswithactivesub- 1. Other projects synthesis,IvanHabuš CustomDNA 15. Descriptionandbehavior ofquantumsys- 14. Matterunderextremeconditions, 13. Multidisciplinarysedimentologicalinvestiga- 12. Interactionsofbiomembraneswithamino 11. 10. Modelingofnovelcarbonmaterials, Electronspinresonanceinsystemswith 9. Biophysicsofliporoteininteractionswith 8. Structureanddynamicsof(bio)molecules, 7. Structural andbiologicalinvestigationof 6. Develeopmentandapplicationofmodelsin 5. Dunja Srziæ(Bilateralproject withSlovenia) and complexesbymassspectrometry, Slovenia) Vesna Noethig-Laslo(Bilateral projectwith (Bilateral projectwithSlovenia) polymer networks,Mladen Andreis project withGermany) bial Lipases,BiserkaKojiæ-Prodiæ,(Bilateral S. Tomiæ (BilateralprojectwithGermany) tion, andInhibitionbyCOMBINE Analysis, ect with Austria) stances, GretaPifat-Mrzljak(Bilateralproj- tems ininteraction, Tomislav Živkoviæ Slobodan Bosanac tions, HalkaBilinski acids andpeptides,Vesna Nöthig-Laslo Ante Graovac paramagnetic particles,BorisRakvin active substances,GretaPifat-Mrzljak Biserka Kojiæ-Prodiæ Ljerka Tušek-Božiæ new complexcompounds, Nenad Trinajstiæ chemistry andbioinformatics, .A.Ce.Soc Chem. Am. J. nance” net Mn ing magneticfieldsoncrystalsofthenanomag- B. Rakvin,D.Žiliæ,J.M.North,N.S.Dalal“Prob- Langmuir tions ofDissymmetricGeminiSurfactants”, of theSpacerLengthonSolidPhase Transi- Puciæ, I.Primožiè,N.Filipoviæ-Vincekoviæ, “Effect Sikiriæ,I.Šmit,Lj. Tušek-Božiæ, V. Tomašiæ, I. M. eral Triangular Lattice:Cu the Doublet-StatesofaSpin-FrustratedEquilat- Symmetry LoweringandElectronLocalizationin Rakvin, C.M.Ramsey, M.Chris,“Observationof B. Cage,F.A. Cotton, N.S.Dalal,E.A.Hillard,B. pean Journal Pure EnantiomerGelation”. Supramolecular Organization.Racematevs Oxalyl Amide Gelators-GelationPropertiesand Kojiæ-Prodiæ, M.Žiniæ,“ChiralBis(Amino Alcohol) J. Makareviæ,M.Jokiæ,Z.Raza,Štefaniæ,B. Acta, Thiol GroupsinHumanLDL”, “The StudyofStructural Accessibility ofFree M. Kveder, A. Kriško,G.Pifat,H.J.Steinhoff, their Properties Radicals: NewInformationon Transients and Reaction of2,4,5-Trichlorophenol withHydroxyl M. Bonifaèiæ,K.-D. Asmus, K.A.Gray, “Onthe somes” and adamantyltripeptidesentrappedintolipo- of immunomodulatingpeptidoglycanmonomer spinlabelingstudy Mirosavljeviæ, J. Tomašiæ “A R. Frkanec,V. Noethig-Laslo,B.Vranešiæ, K. Artemisinine”, important drugmolecules:Zidovudine& I. Novak,B.Kovaè,“Photoelectronspectraof 1307. 2003 SELECTED PUBLICATIONS 12 .Mg Res J. Mag. ici.Bohs Acta Biophys. Biochim. -acetate byelectronparamagneticreso- 2003 , 1631 . 2003 .Og Chem Org. J. , .J hs Chem. A Phys. ”. J. 19 , 239. . , 10044. , 2003 . 9 2003 , 5567. , 3 125 (O , . 165 7 2003 hmsr- Euro- Chemistry-A , 5270. C ici.Biophys. Biochim. 16 2003 , 260 H , 23 68 , , )x1.2C 2003 1611 , 5777. , , 187. 6 107 H 12 ” , Division of Organic Chemistry and Biochemistry ZOKB

http://www.irb.hr/str/zokb

DIVISIONAL ORGANISATION OVERVIEW OF THE DIVISION Head: Mladen Žiniæ Research activities within the division during 2003 resulted in the publication of an The Division of Organic Chemistry and impressive number of scientific papers, most Biochemistry (ZOKB) consists of the fol- of which appeared in reputed international lowing laboratories: journals. The topics of scientific investigation varied widely and included fields such as D Laboratory for stereoselective synthesis, physical organic chemistry, catalysis and biocatalysis, supramolecular chemistry, molecular spec- Vitomir Šunjiæ troscopy, cellular biochemistry, and quantum D Laboratory for synthetic organic organic chemistry. The members of the divi- chemistry, Kata Majerski sion actively participated in the organizing th D Laboratory for supramolecular and committee for the 13 European Symposium nucleoside chemistry, Mladen Žiniæ on Organic Chemistry (ESOC 13), held in Cavtat near Dubrovnik. This highly success- D Laboratory for carbohydrate, peptide and glycopeptide chemistry, ful meeting attracted 455 participants from Štefica Horvat 40 different countries all over the world. The scientists of the division also made a signifi- D Laboratory for cellular biochemistry, Maja Abramiæ cant contribution to higher education in organic chemistry by providing 9 courses, at D Laboratory for physical organic undergraduate and postgraduate levels, and chemistry, Mirjana Eckert-Maksiæ 2003. report annual RBI by supervising 4 M.Sc. and 5 Ph.D. theses. D Laboratory for molecular spectroscopy, Goran Baranoviæ TOP ACHIEVEMENTS D Group for quantum organic chemistry, Zvonimir Maksiæ Supramolecular chemistry of gels The formation of nano-fibers in organo- gels of bis(amino alcohol) oxalamides was

35 36 RBI annual report 2003. ZOKB Makareviæ vectors) inthree-dimensionalspace(J. ality ofaggregateforces(supramolecular result fromdiffering strengthsanddirection- cules intofibrousaggregateswasshownto elucidated. The self-assembly ofsuchmole- ment ofnewtechnologiessuchasslowdrug materials” areofinterestforthedevelop- 5567). Supramoleculargelsasnew“smart et al. Figure 1. , Chem. Eur. J. TEM images:gelfibrescanbetranscribedintosilicanano-tubes(left) molecules ofsolvent. hardens upto40.000 of anorganogelator Figure 2. electronics. for catalysisor inorganic materials nano-dimensional the preparationof liquids orfuelsand port ofdangerous safer surfacetrans- tissue engineering, delivery systems, One molecule 2003 or twistedribbons(right). , 9, tion energycalculatedby of adirectrelationshipbetweenthecorrela- trons inmoleculesresultedthediscovery Quantum organicchemistry and Rydbergstates(I. Antol tions, appliedtothelowest excitedvalence revealed throughstate of theartcalcula- tation spectrum. These effects were important ramificationsforitselectronicexci- group offormaldehydewasshowntohave Photoprotonation studies sible. demanding lation energyinlargermolecules,forwhich anaccurateestimationofthecorre- enables exhibits aremarkableatomicadditivitywhich that theenergyofelectroniccorrelation Chem. A Theory (D.Bariæ,Z.B.Maksiæ, ogy andthatobtainedbyDensityFunctional Studies ofthecorrelationenergyelec- The protonationstateofthecarbonyl , 2003 ab initio , 107 calculations arenotfea- , 11577). Itwasshown ab initio et al.Chem. methodol- J. Phys. ZOKB RBI annual report 2003. 37 anticancer ; inventors: Bis- in vitro 2003 , Apparatus for microwave assisted synthesis. EP 0 877 022 Figure 4. -1-sulfonylpyrimidine derivatives synthesized PATENT AWARDS N Mladen Žiniæ was awarded the Croatian Acad- Mladen Žiniæ was awarded the Croatian New compounds with antitumor activity New compounds with antitumor cell lines activity against various human tumor against normal while their cytostatic effects Euro- lower. human fibroblasts were significantly pean Patent ( in the Laboratory for supramolecular and nucleo- in the Laboratory for supramolecular side chemistry showed strong erka Žiniæ, Mladen Žiniæ, Irena Krizmaniæ,) has erka Žiniæ, Mladen Žiniæ, Irena Krizmaniæ,) with been granted for new sulfonylpyrimidines The compounds are promising antitumor activity. candidates for development of new anticancer drugs. Arts prize for scientific emy of Sciences and achievement in natural sciences -chemistry. Irena Zrinski won the annual award for young Teach- The Society of University scientists from , Biopolymers , 587), and provide an and provide , 587), 374 Calculated electronic spectrum 2003, 2003, of protonated formaldehyde. Figure 3. , 69, 421-431). An extensive study of glycation products An extensive study implicated in the pathogenesis of diabetic implicated in the Alzheimer’s dis- and complications, aging, facts about the stability ease, revealed new products of the glycated and degradation leucine-enkephalin under peptide hormone Aphysiological conditions. new chemical lead- transformation of the glycated peptide, amino ing to beta-scission of the terminal in reac- acid side-chain, was found to result impair cell tive aldehyde species that can viability (A. Jakas, Š. Horvat, Carbohydrate and peptide chemistry Carbohydrate and important stepping-stone on the way to a the way on stepping-stone important pro- photoinduced of deeper understanding in complex, biologi- processes ton transfer molecules. cally active 2003 Phys. Lett. Phys. 38 RBI annual report 2003. ZOKB lent ModificationsofPeptidesandProteins.13 Exploring Sugar-MediatedNonenzymaticCova- Štefica Horvat,SyntheticGlycopeptidesin Synthesis, StructureandProperties”,2 Construction ofCrownEthersandCryptands: Kata Majerski,“AdamantaneasBuildingBlockin SA, 13-16.04.2003. Conference, RhodesUniversity, Grahamstown, Species, FRANKWARREN OrganicChemistry Generation andFateofCarbeneDicarbene Kata Majerski,CarbenesinPolycyclicSystems. (Cavtat-Dubrovnik, Croatia,10-15.09.2003.) European SymposiumonOrganicChemistry nal ofChemistry important publishedscientificpaper( ers andScientists,Zagreb,Croatia,forthemost . Protonaffinity andtheprotontransferreac- 9. 8. Extended Hydrolases–fromisolationtofunction, 7. Reactiveintermediatesingroundandexcit- 6. Design and synthesisofbioactivepeptides, 5. Supramolecular organization ofgels, 4. Synthesis,molecularstructureandfunction 3. Newopticallyactivematerials, 2. Stereoselectivesynthesisandcatalysis, 1. and Technology (MZT) Projects supportedbytheMinistryofScience Varaždin, Croatia,19-21.06.2003. European ChemicalMeeting(CECM-2) INVITED LECTURES PROJECTS tion inchemistry, ZvonimirMaksiæ troscopies, GoranBaranoviæ Maja Abramiæ ed state,MirjanaEckertMaksiæ Štefica Horvat glycopeptides andbiomarkers, Mladen Žiniæ molecular recognitionandcatalysis, of polycyclicmolecules,KataMajerski Vladimir Vinkoviæ Vitomir Šunjiæ π -systems andmolecularspec- 2003 , 8 , 1270). New Jour- nd Central th 3Y Scheme, B3LYP Correlation EnergyinMoleculesbytheDFT- D. Bariæ,Z.B.Maksiæ, Atomic Additivity ofthe 9, 5567-5580. Pure EnantiomerGelation, Supramolecular Organization:Racemateversus hol)oxalamide Gelators–GelationPropertiesand Kojiæ-Prodiæ, M.Žiniæ,ChiralBis(aminoalco- J. Makareviæ,M.Jokiæ,Z.Raza,Stefaniæ,B. Potentialantitumordrugs,ŠteficaHorvat, 1. Research anddevelopmentprojects MirjanaEckert-MaksiæandZvonimir 4. Syntheses,structureandmechanismsof 3. IntrinsicReactivityofNewMolecularMateri- 2. Propertiesofnewmolecularmaterials, 1. International andcollaborativeprojects Spectroscopic Properties. Smaller Fragments:Stability, reactions and cation ofOpioidPentapeptideandRelated ways of Amadori CompoundsObtainedbyGly- A. Jakas,Š.Horvat,StudyofDegradationPath- ters. tonated formaldehyde, H. Lischka,Valence andRydbergstatesofpro- I. Antol, M.Eckert-Maksiæ, T. Mueller, M.Dallos, 11577. ai-aesi Cyclopropyl-Homoallyl Rearrangement Accompanying theBorane- nariæ-Majerski, A G. Kragol,I.Benko,J.Muharemspahiæ, K.Mli- 69, 421-431. SELECTED PUBLICATIONS (MZT, HITRA, TP-B23/2001) tia-Austria andCroatia-Sloveniaprojects. Makasiæ alsocollaboratein3bilateralCroa- ject coordinatorMladenŽiniæ systems, Collaborativeproject,MSES,pro- functional molecularandsupramolecular Zvonimir Maksiæ D26, als, Working groupwithinCOST Mirjana Eckert-Maksiæ D26, Working groupwithin COST 2003 , 374,5-6;587-593. J. Phys.Chem. A Chemical PhysicsLet- Chem. Eur. J. Biopolymers , 2003 , , 2003 2003, 107 , , ZOKB RBI annual report 2003. 39 Gyne- , 661-662, 2003 , 194-200. , 194-200. 91 , 2003 , J. Mol. Struct. siveness of ovarian primary carcinomas, carcinomas, primary of ovarian siveness Oncology cologic N. Biliškov, B. Zimmermann, G. Baranoviæ,. Zimmermann, B. N. Biliškov, macrocyclic of spectroscopy Vibrational A – I. oligo(phenyldi-acetylenes) and theoretical octadehydrodibe- study of experimental and dodecadehydrotriben- nzo[12]annulene zo[18]annulene, 65-80. Eur. J. Eur. Tetrahedron Asym- Tetrahedron , 2622. 2003 , 14, 2725-2730. . 2003 . Mediated Reduction of Tosylhydrazones, Tosylhydrazones, of Reduction Mediated Chem Org. metry M. Majeriæ-Elenkov, Z. Hameršak, V. Šunjiæ, V. Z. Hameršak, M. Majeriæ-Elenkov, Racemates of Diastereomeric Kinetic Resolution of 7-Bromo-3-(1`-hydroxy-ethyl)-1-methyl-5-(2`- 4-benzodiazepin-2- 3-dihydro-1H-1, pyridyl)-2, CAL-B. one by Immobilized Š. Šimaga, D. Babiæ, Osmak M, Šprem M. Š. Šimaga, D. Babiæ, cytosol dipeptidyl peptidase Tumor Abramiæ M., with histological aggres- III activity is increased

Division of Materials ZKM Chemistry

http://www.irb.hr/str/zkm

pounds, organic polymers, intermetallic DIVISIONAL ORGANISATION compounds, and metal hydrides. This was Head: Svetozar Musiæ achieved through the application of a variety of established techniques, including the The Division of Materials Chemistry kinetics and mechanisms of precipitation, (ZKM) consists of the following and the biochemical research of fish, shell- laboratories: fish and crustacean tissues, as well as through the development of new and exist- D Laboratory for synthesis of new ing theoretical and experimental methods in materials, Boris Subotiæ materials science. In addition to the 50 or so D Laboratory for precipitation processes, companies that used our established radia- Ljerka Breèeviæ tion services for sterilization, pasteurization, D Laboratory for radiation chemistry and desinsection and/or decontamination, the dosimetry, Dušan Ražem division participated in numerous coopera- D Laboratory for solid state chemistry, tive activities with different industries, hospi- Želimir Blažina tals, and state institutions.

D Laboratory for complex compounds chemistry, Pavica Planiniæ TOP ACHIEVEMENTS D Group for ichtiopathology – biological materials, Rozelinda Èož-Rakovac A new zeolite microstructure

Zeolites, particularly those with control- 2003. report annual RBI lable microstructure, have the potential to be OVERVIEW OF THE DIVISION used for the storage of various organic compounds. In our laboratories one such materi- The Division of Materials Chemistry con- al was synthesized by controlled heating of tinued to focus on obtaining a better under- the NH4-exchanged zeolite A4. This process standing of the properties of materials such initially resulted in an amorphous phase, as magnetic and nonmagnetic metal oxides, which was then replaced by a mixture of oxide glass-ceramics, zeolites, cluster com-

41 42 RBI annual report 2003. ZKM the firsttime,thatultrafineiron-doped TiO (SCCVII culturedinvitro)haveshown,for Experiments withsquamouscarcinomacells activity againstcancercells Nano-sized metaloxideparticlesshow Mater. Kosanoviæ, B.Subotiæ, formation ofmullitemicro-vessels(C. are otherwiserelativelyinert. The mostpro- effects inthepresenceofUVirradiation,and particles displayappreciablecytotoxic mullite andamorphoussilica(SiO SiO resulted intheextractionofamorphous treatment ofthisfinalproductwithalkali 2 Figure 2. from theinteriorofeachparticleand , 2003 Figure 1. , Control ofzeolitecrystallization 2-3 , 311). Mullite micro-vessels Micropor. Mesopor. 2 ). The 2 ta.J.Sol-GelSci.Technol. et al. the Fe/Ti molarratioof0.136(S.Ivankoviæ nounced photokillingeffect wasobservedfor effects ofirradiation(M.Denac the parallel separatedtalc crystals reduce addition, wewereabletoshowthatplane- tational growthofpolypropylenelamellae.In matrixaswellaffecting orien- tion ofiPP disturbed thewell-developedspherulitiza- ites hasrevealedthatplate-liketalccrystals Our recentcharacterizationofsuchcompos- superior plasticsforindustrialapplication. composites isimportantforthedesignof ships ofisotacticpolypropylene(iPP)/talc The studyofthestructure-propertyrelation- polypropylene/talc composites Orientational growthin Phys. Chem pulse (B.Mihaljeviæ,D.Ražem, plex onthemicrosecondtimescaleafter absorption ofthemonochloroiron(III)com- was monitoredbyobservingtheoptical of dibutylperoxide. The formationofiron(III) ding totheabsorptionofperoxidebond laser impulseatthewavelengthcorrespon- butoxyl radicalwasgeneratedbyapplyinga iron(II) ioninmethanol. The modeltert- the reactionofamodelalkoxylradicalwith of competitionkinetics,therateconstantfor we havesucceededtomeasure,bymeans zation hasthusfarprovenelusive. This year, is extremelyrapidanditskineticcharacteri- tant reductionofhydroperoxideswithiron(II) Ultrafast redoxchemistry Degrad. Stabil. The secondstepinthebiologicallyimpor- ., 2003 2003 , 67 , 82 , 269). , 263). 2003 et al. , 27 , Radiat. Polym. , 225). ZKM RBI annual report 2003. 43 ] has 6 ) 3 J. Alloys , Polyhedron , (OCH 8 et al. et al. -Br) 3 µ ( 6 , 501). [Mo 2 ] 5 356/357 OH) 3 , 1553). 2003, 2003, 22 . Stability by Radiation Grafting”, Internation- al Meeting on Radiation Processing, Chica- go, September 7-12, 2003. Unsaturated Polyesters”, Polymer Science and Engineering, University of Massachu- setts, September 4, 2003. in Unsaturated Polyester Transitions In an effort to better understand the to better understand In an effort INVITED LECTURES age purposes (B. Šorgiæ (B. purposes age Comp A of a crystal structure new cluster methoxo molybdenum organometallic compounds unique nature of metal-metal bond, two possessing a direct methoxo clusters of series of polymeric tungsten have been syn- molybdenum and first series, the methoxo thesized. In the between neighbouring groups act as bridges the second series shows cluster units while groups. both bridging and terminal methoxo clus- The crystal structure for the monomer ter [Na(CH 1. Ranogajec, “Improvement of Polymer F. 2. in Transition “Liquid-liquid Ranogajec, F. 3.“Crosslinking and Structural Ranogajec, F. been solved. (N. Brnièeviæ 2003, International training course to In addition to the established contribution at undergraduate and graduate education the divi- the universities of Split and Zagreb, training course to a specialized sion offered Atomic Energy a fellow of the International sterilization Agency in the area of radiation of pharmaceuticals. (RE = x structure Al 2 5-x Ga 3 type is preserved up 5 Moessbauer spectrometer for materials research -hydrogen (RE= Tb, Y) and Tb, -hydrogen (RE= 0.5 Metal ion hydrolysis forced by ultrasound Metal ion hydrolysis Al Figure 4. Al-hydrogen (RE=Gd, Tb, Y, Dy, Ho) Dy, Y, Tb, Al-hydrogen (RE=Gd, 4.5 4 The crystal structure and hydrogen sorp- The crystal structure and hydrogen Figure 3. are attractive candidates for hydrogen stor- are attractive candidates for hydrogen RENi to x=2, while beyond this composition a to x=2, while beyond this composition YCo transformation into the tion properties of the new RENi A hydrogen new type of materials for storage type occurs. Taking into account the hydride Taking type occurs. alloy for- capacity of at least 3.5 H atoms per pres- mula unit and the suitable equilibrium systems sure at ambient temperature, the RENi Gd, Tb, Dy, Ho, Er and Y) alloys were inves- Ho, Er and Tb, Dy, Gd, prototype tigated. In all systems the binary structure of the CaCu Resins”, Kansas Polymer Research Center, SELECTED PUBLICATIONS Pittsburg State University, Kansas, Septem- ber 15, 2003. B. Subotiæ, J. Broniæ, “Theoretical and Practical 4. S. Musiæ, “Moessbauer Spectroscopy in Aspects of Zeolite Crystal Growth”. S. M. Auer-

ZKM Chemistry”, Croatian Academy of Science bach, K. A. Carrado and P. K. Dutta (Eds.), and Arts, Zagreb, November 18, 2003. “Handbook of Zeolite Science and Technology”, Marcel Dekker Inc., New York, Basel, 2003, pp. PROJECTS 129-203.

Projects supported by the Ministry of C. Kosanoviæ and B. Subotiæ, “Preparation of Science and Technology (MZT) Mullite Micro-vessels by a Combined Treatment of Zeolites”, Micropor. Mesopor. Mater., 2003, 2- 3, 311. 1. Research of the crystallization process and use of zeolites, Boris Subotiæ S. Ivankoviæ, M. Gotiæ, M. Jurin, S. Musiæ “Pho- 2. Kinetics and mechanisms of solid phase tokilling Squamous Carcinoma Cells SCCVII with precipitation from electrolyte solutions, Ultrafine Particles of Selected Metal Oxides”, J. Ljerka Breèeviæ Sol-Gel Sci. Technol. 2003, 27, 225. 3. Synthesis and microstructure of metal oxides and oxide glasses, Svetozar Musiæ B. Mihaljeviæ, D. Ražem, “Oxidation kinetics of 4. Physico-chemical effects of ionizing radia- iron(II) ion with t-butoxyl radical”, Radiat. Phys. tions, Dušan Ražem Chem., 2003, 67, 269. 5. Synthesis, characterization and modifica- M. Denac, V. Musil, I. Šmit, F. Ranogajec, tion of polymers by ionizing radiation, “Effects of talc and gamma irradiation on Franjo Ranogajec mechanical properties and morphology of isotac- 6. Intermetallic compounds and metal tic polypropylene/talc composites”, Polym. hydrides, Želimir Blažina Degrad. Stabil. 2003, 82, 263. 7. Superconducting oxides and polynuclear metal complexes, Nevenka Brnièeviæ B. Šorgiæ, A. Drašner, Ž. Blažina, “Structural and

8. Influence of dopands on the structure and hydrogen sorption properties of RENI5-xAlx (RE= properties of materials for technical applica- Gd, Tb, Dy, Ho, Er and Y) alloys.”, J. Alloys tions, Biserka Gržeta Comp. 2003, 356/357, 501) 9. Development of an adaptable technological procedure for the production of precipitated N. Brnièeviæ, I. Bašic, B. Hoxha, P. Planiniæ, R.E. calcium carbonate, Damir Kralj (HITRA) McCarley, “Molybdenum and Tungsten Methoxo 10. Characterization of the aluminate cement Clusters with Differently Bonded Methoxo clinker by the Rietveld method, Groups. Crystal Structure of Biserka Gržeta (HITRA). [Na(CH3OH)5]2[Mo6(µ3-Br)8(OCH3)6]”, Polyhe- dron 2003, 22, 1553. RBI annual report 2003.

44 Division of Molecular ZMB Biology

http://www.irb.hr/str/zmg

DIVISIONAL ORGANISATION OVERVIEW OF THE DIVISION Head: Ðurðica Ugarkoviæ In 2003, the Division of Molecular Biology The Division of Molecular Biology (ZMB) made several major discoveries in basic consists of the following laboratories: research areas such as bacterial recombination and translational control. The installa- D Laboratory of Microbial Genetics, tion of a new confocal microscope will Erika Salaj-Šmic expand the scope of possible projects in D Laboratory of Molecular Microbiology, many of the research groups. The division Mirjana Petranoviæ was responsible for the organization of the D Laboratory for Molecular Genetics, highly successful Methodological Courses in Vera Gamulin Biology and Medicine. In recognition for her efforts as the head of the Molecular Genet- D Laboratory for Molecular Genetics of Eukaryotes, Miroslav Plohl ics Laboratory, Vera Gamulin was awarded the Croatian state prize for significant contri- D Gene Regulation Laboratory, Mary Sopta butions to the development of molecular biology research in Croatia. D Laboratory of Experimental Cancerology, Ivica Rubelj TOP ACHIEVEMENTS D Laboratory for Genotoxic Agents, Maja Osmak Fidelity enhancement of RNA synthesis D Laboratory for Neurochemistry and by small proteins B nulrpr 2003. report annual RBI Molecular Neurobiology, In collaboration with D. Soll’s group at Branimir Jernej Yale University, I. Ahel discovered small D Laboratory for Electron Microscopy, autonomous proteins which enhance the Nikola Ljubešiæ fidelity of aminoacyl-tRNA synthesis. These D Laboratory for Chemical Biology, editing modules could represent a link Volker Magnus between the ancestral aminoacyl-tRNA syn- D Laboratory for Biocenotic thetases and extant enzymes (I. Ahel et al., Investigation, Andrija Željko Lovriæ Proc. Natl. Acad. Sci. U. S. A., 2003, 100, 15422).

45 46 RBI annual report 2003. ZMB Weber, Institute inMartinsried(Figures2,3,4;I. duced byIgorWeber fromtheMaxPlanck expand anexcitingnewresearchareaintro- 1). Itisplannedtousethisequipment tion ofaLeicaconfocalmicroscope(Figure sion wasimprovedin2003bytheinstalla- New confocalmicroscope the sameauthors. recombinational pathwayswasproposedby explains theinteractionsofthesetwo Genetics, obtained intheZMB(I.Ivanèiæ-Baæe other, undercertainconditions,was and RecF)tofunctionallyreplaceeach pendent recombinationalpathways(recBCD bility forenzymesderivedfromtwoinde- pathways Interaction oftworecombinational tigious scientificrecognitionavailablein Award toVera Gamulin 34). The microscopyinfrastructureoftheDivi- The firstexperimentalproofforthepossi- The CroatianState Award, themostpres- Figure 1. Methods ofEnzymology 2003 Confocal microscopeattheDivision of MolecularBiology , 163 , 485). The modelwhich , 2003 et al. , 361 , , ria structure ofmarinespongesandthebacte- Molecular Biology, forresearch onthegene Gamulin, seniorresearcherattheDivisionof Croatia, waspresentedthisyeartoVera were organizedbytheZMB incollaboration ological coursesinBiology andMedicine molecular biologyinCroatia, theMethod- Novel initiativeineducation coordinated byV. GamulinandN. Tvrtkoviæ. was initiatedbytheZMB. This projectis groups fromdifferent institutionsinCroatia, and breeds”involving20research fauna andeconomicallyimportantspecies characterization ofautochthonousfloraand entitled “BiodiversityinCroatia:genetic Biodiversity inCroatia Institute in1982. research programattheRuðerBoškoviæ technologyintothe recombinant DNA research inCroatiaforherintroductionof ognized asapioneerofmolecularbiology Figure 2. by Dictyosteliumamoebaetranfectedwithaprotein To improve theeducationinfieldof largecollaborativeresearchproject, A Streptomyces rimosus marker forendoplasmaticreticulum Phagocytosis ofopsonizedyeastparticles ue oGP(green). fused toGFP . Sheisalsorec- ZMB RBI annual report 2003. 47 genome stability, Erika Salaj-Šmic genome stability, Mirjana Petranoviæ national repair, species, served and economically important Gamulin Vera Ðurðica Ugarkoviæ Plohl sequences in invertebrates, Miroslav Mary Sopta cellular aging, Ivica Rubelj Brdar minogen serine proteases, Branko logical noxa, Maja Osmak transmission, Branimir Jernej Darko Oreškoviæ cytoskeleton, Nikola Ljubešiæ Magnus cules, Volker Andrija Željko Lovriæ and their mycoflora, PROJECTS posium on November 20-21 at the Ruðer at the 20-21 November on posium entitled symposium, This Institute. Boškoviæ - Biology in Croatia of Molecular “45 years notable inter- Helix”, included 50 of Double D. Erlich, R. Lloyd, contributions by national and Schaffner W. M. Radman, Mueller, W. H. Schwab. Projects supported by the Ministry of Projects supported (MZT) Science and Technology 1. The role of recombination in DNA repair and 2. Regulation of recombination and recombi- 3. Genes and genomes of evolutionarily con- 4. Evolutionary dynamics of satellite DNAs, 5. Organization of heterochromatic DNA 6. regulation in eukaryotes, Transcriptional 7. Molecular mechanisms of imortalization and 8. Structure, function and regulation of plas- 9. Cell response to physical, chemical and bio- 10. of serotonergic Molecular pathophysiology 11. Hydrodynamics of the cerebrospinal fluid, 12. Structure and function of plastids and 13. Dynamics and genetics of bioactive mole- 14. Endemic and relict phytocenoses of Croatia th anniversary of the dis- th Cell division of multinuclear mutant Cell division of multinuclear are labeled by DAPI (blue). To mark the 50 To the cytoskeleton (red) and microtubuli (green and the cytoskeleton (red) for the motor protein myosin II. Cortical proteins of for the motor protein yellow) are labeled by immunofluorescence. Nuclei yellow) are labeled by immunofluorescence. Figures 3 and 4. Dictyostelium cells with deletion of a gene that codes Dictyostelium cells with anniversary of the commencement of molecular biology in Croatia, the ZMB (A. Ferle- and E. Salaj-Šmic) organized a sym- Vidoviæ 50 years of double helix with the Divisions of Molecular Medicine, with the Divisions of Molecular and Organic Chemistry and Biochemistry, Boškoviæ Marine Research from the Ruðer and The courses were initiated Institute. lead by A. Ambrioviæ-Ristov. covery of the DNA double helix and the 45 48 RBI annual report 2003. ZMB 0 Studiesonserotonintransporter genestruc- 10. Serotoninandimmunefunctions:studieson 9. Effect offibronilytictreatmentinexperimen- 8. FunctionalcharacterizationoftheXtc1pro- 7. Humanadenovirustype5retargetedon 6. Diazenesaspotentialanti-cancerdrugs 5. Biologicalresponsetodamage,OsmakM– 4. PlantHormones-Physiology, Biochemistry 3. .Usageoftelomeraseinrevitalizationcells 2. BiodiversityinCroatia:geneticcharacteri- 1. Collaborative projects ect Medicine, UniversityofBonn), bilateralproj- Jernej, B.;Wildenauer, D.B(Schoolof ture andexpressionin schizophrenia, project Karl-Franzens University, Graz)bilateral for GeneralandExperimentalpathology, Jernej, B.(IRB);Schauenstein,K(Institute manipulations ofserotoninhomeostasis, rats afterpharmacologicalandgenetic ier UniversitaireVaudois, Lausanne) Brdar, B.(IRB),Busso,N. (CentreHospital- Grant, SwissNationalScienceFoundation, tal modelsofrheumatoidarthritis,SCOPES lar Biology) (University ofZuerich,InstituteforMolecu- Foundation, Sopta,M.(IRB),Schaffner, W. SCOPES Grant,SwissNationalScience tein of Ambrioviæ-Ristov A. andEloitM. Grant NationaleVeterinaire d’Alfort,France, Science Programme,Collaborativelinkage aminopeptidase (2002-2004)NATO collaboration jana, (OsmakM.andPolancS),bilateral Chemical Technology, UniversityofLjubl- (2001-2003), FacultyofChemistryand (2003-) collaborative project,MZT project, MZT, (2003- ) and Application, MagnusV- collaborative laborative project,MZT, (2003-) and tissues ect, MZT, (2003-) Gamulin V, Tvrtkoviæ, N–collaborativeproj- economically importantspeciesandbreeds, zation ofautochthonousfloraandfauna in vitro Saccharomyces cerevisiae, and in vivo , RubeljI-col- editing ofmischargedtRNAs Ahel, I.;Korenèiæ,D.;Ibba,M.;Soll,D. Trans- RoleofRecBCDenzymeincellviability, 14. Comparativestudyofthedistribution,struc- 13. Molecularenzymologyandenzymeengi- 12. Geneticstudiesonsynapticelementsin 11. Sci. U.S. A Escherichia coli loading-deficientrecBmutantof nation inaRecA repairandrecombi- function isrequiredforDNA bot N.;Salaj-ŠmicE.;Brèiæ-KostiæK.RecFOR Ivanèiæ-Baæe, I.;Peharec,P.; Moslavac,S.;Škro- Psychiatry in suicidevictimsofCroatianorigin. (5-HTTLPR) andintron2(VNTR)polymorphisms Jernej B.Serotonintransportergenepromoter Hraniloviæ D,ŠtefuljJ,FuraèI,KubatM,Balija ing andnon-expressing cells. efficiency ofpost bindingstepsinCAR-expressing adenovirustype5fibershaftdecreasesthe Ambrioviæ-Ristov A, Mercier S,EloitM:Shorten- 312 SELECTED PUBLICATIONS , 425-433. Croatia (ALIS), BritishCouncilandMZT, Republicof demic LinksandInterchangeScheme ty ofNottingham,InstituteGenetics): Aca- Salaj-Šmic, E.(IRB),Lloyd,R.G.(Universi- COGITO bilateralproject Sereno, P. (INRA, Antibes, France) reproduction, Plohl,M.(IRB),Castagnone- nematodes inrelationwiththeirmodeof sequences inthegenomeofroot-knot ture andevolutionofsatelliteDNA Institute ofBiotechnology)bilateralproject Schwab, H.(GrazUniversityof Technology, neering ofhydrolases,Vujaklija, D.(IRB), University ofMunich)bilateralproject chemistry Department,PsychiatricHospital, ders, Jernej,B.(IRB),Bondy, B.(Neuro- healthy populationandpsychiatricdisor- , ., 2003 2003 . , Genetics, 54 , 100 , 884-889. , 15422-15427. 2003 . Proc. Natl. Acad. , Virology 163 , 485-494. Biological , 2003 , ZMB RBI annual report 2003. 49 , 193-204. 50 , 2003 ., Viguera E, Petranoviæ M, Zahradka D, Germain M, Zahradka Petranoviæ E, Viguera poly- of bypass Lethality Michel B. DS, K, Ehrlich cells with a defective in Escherichia coli merases DNA complex of clamp loader III. polymerase Mol. Microbiol , Gene species-groups (Coleoptera). (Coleoptera). species-groups , 85-94. 312 , Pimelia 2003 Bruvo B., Pons J., Ugarkoviæ Ð., Juan C., Petit- Juan Ð., J., Ugarkoviæ B., Pons Bruvo number low-copy of M. Evolution E., Plohl pierre satellite DNAand major in sequences coexisting two

Division of Molecular ZMM Medicine

http://www.irb.hr/str/zmm

DIVISIONAL ORGANISATION D Laboratory of functional genomics, Head: Krešimir Paveliæ Marijeta Kralj D Laboratory of molecular virology and The Division of Molecular Medicine bacteriology, Magdalena Grce (ZMM) consists of the following laboratories:

OVERVIEW OF THE DIVISION D Laboratory of cellular and molecular immunology, Mariastefania Antica The mission of the Division of Molecular D Laboratory of molecular oncology, Medicine is to expand and strengthen the Jasminka Paveliæ knowledge of the nature of disease and to develop and improve new strategies for the D Laboratory of molecular pathology, Koraljka Gall-Trošelj diagnosis, treatment and prevention of disease. These goals are realized through the D Laboratory of experimental evaluation of the impact of genetic factors in hematology, immunology and oncology, Jelka Gabrilovac disease prevention, the reduction of risk factors, the development and evaluation of new D Laboratory of biological response drugs, the exploration of the biochemical modifiers, Tatjana Marotti and cellular effects of drugs on cells and liv- D Laboratory of immunochemistry, ing organisms, the improvement of scientific Biserka Pokriæ methodology, and the education of scientists B nulrpr 2003. report annual RBI D Laboratory of molecular endocrinology and students. and transplantation, Mirko Hadžija The Division is currently developing sever- D Laboratory for oxidative stress, al strategic projects. These range from the Neven Žarkoviæ development of gene therapy for use against cancer to new diagnostic and D Laboratory of molecular neuropharmacology, Danka Perièiæ research tools based on transcriptomic and proteomic approaches, as well as tissue

51 52 RBI annual report 2003. ZMM p21 understanding oftheroles Gene therapyofcancer study ofdisease,insouth-easternEurope. for researchinmolecularapproachestothe a preeminentcentre(centreofexcellence), cloning. As such,theDivisionisemergingas engineering forthepurposeoftherapeutic and the relativeantitumoractivitiesof application ingenetherapy. We compared naturally ofgreatimportancefortheirfuture cycle andapoptosis.Suchunderstandingis posed forhumangastriccancertherapy. We of theIGFfamilygenes,hasbeenpro- therapy New strategyforhumangastriccancer as therapeuticgenes.(M.Kralj and evaluatedthepossibilityofusingthem ied theirrolesintheinductionofapoptosis mediated genetransfer. Inaddition,westud- Gene Ther. Figure 1. Our resultshavecontributedtoabetter ACHIEVEMENTS TOP new approach,basedontheblockage A WAF1/CIP1 p21 (left) andafter(right)theintroduction ofp53gene, WAF1/CIP1 The expressionofp53protein inhumantumorcellsbefore genes intheregulationofcell , 2003 genes usingadenovirus- , 10 using adenoviralvectors. 457). , ta.Cancer et al. p53 and p53 tion tobeingableanalyzeawhole use inbasicandappliedresearch.Inaddi- microarray)for expression analysis(DNA implemented intheDivisionforglobalgene powerfulnewtechnology A uptake ofserotonin.(N.Pivac antidepressants thatselectivelyblockthere- action thatissimilartotheeffects ofother serotonin synthesisintheratbrainbyan uptake ofnoradrenaline,affects therateof antidepressant drugthatblocksthere- Mechanisms ofantidepressantdrugs 201, rochem Int. IF1)(K.Paveliæ (IGF 1R) tions betweenIGF2andoneofitsreceptors ed byusingantibodiesthatpreventinterac- autocrine circuitcanbecompletelyinterrupt- type ofcancerthantheintestinaltype. The more pronouncedintheaggressivediffuse the IGF1receptor. The IGF2/IGF1Ris by autocrineIGF-2-mediatedstimulationof noma cellsthatproduceIGF2isregulated discovered thatthegrowthofgastriccarci- powerfulnewtechnologyhasbeen A It hasbeenshownthatdesipramine,an 430). molecular medicine. their ownright,inall fields of important areasofresearch, in tures arebecomingextremely markers. These lattertwofea- analysis ofhighlypolymorphic cific geneclustersaswellthe vant results,furtherstudyofspe- biostatistical profilingoftherele- this methodologyallows,afterthe genome inaslittleoneweek, , 2003 , 43 et al. , 611). , J. Pathol. et al. , , 2003 Neu- , ZMM RBI annual report 2003. 53 et al. Avian et al. production of skin trans- 59). in vitro The Abi Prism 310 Genetic Analyzer Abi Prism 310 Genetic The 47, cultivation of human keratinocytes cultivation of human , production of skin transplants production of skin 2003 Figure 3. In vitro The Abi Prism 7000 Sequencing Detec- The In vitro has been adopted and without feeder layer model for the applied as an experimental The pro- cell differentiation. investigation of cedure for plants for clinical application has been mas- plants for clinical application has tered. New equipment expression tion system apparatus, for gene been imple- analysis (real time PCR) has The mented for basic and applied research. Cary Spectrophotofluorimeter Varian was purchased with the ECLIPSE (VARIAN) Ministry of financial help of the Croatian instrument is This Technology. Science and responsible for the outbreak of the disease of the outbreak for the responsible loss in economical a significant in resulting a 5-year period. production during chicken of for the design were applied The results and schedules both immunoprophylactic Lijkiæ vaccines (I. appropriate Dis. screening of in vitro Laboratory of Functional Genomics Laboratory of Functional Genomics of Functional Laboratory For the first time in Croatia, the restriction The establishment of “The center for inte- The establishment of “The center The Figure 2. enzyme analysis of the reverse transcrip- tion/polymerase chain reaction products of viral genome segments was applied for the of field isolates of animal differentiation viruses. Infectious bursal disease virus is Application to animal viruses grative genomics, molecular diagnostics, grative genomics, molecular 000 HRK and gene therapy”, worth 6 000 Dragan Pri- and led by Krešimir Paveliæ and morac, has been recently approved. Megaproject Core potential antitumor compounds. potential antitumor was established and partially equipped in equipped and partially was established the imple- addition to facilitating 2003. In genomics and pro- mentation of functional this laboratory is teomics methods, equipped to allow The establishment of the Laboratory of of the Laboratory establishment The Genomics Functional 54 RBI annual report 2003. ZMM projects andbusinessplansinbiomedicine. entitled gram ofBiomedicineandHealthSciences ological coursewithinthePh.D.studypro- The Divisionintroducedthenovelmethod- es forthePh.D.studyprogram.Inaddition, which theDivisionsubmitted21newcours- (http://bio.mef.hr/docs/Publication.pdf) into Medicine joint fieldofdoctoralstudyin University ofZagreb,theZMMestablisheda Education cence ofvarioussamples. phosphorescence andbio/chemolumines- used forthemeasurementoffluorescence, . The SHH/PTCH/SMO signallingpathwayin 3. Moleculargeneticsofgastrointestinal 2. Molecularmechanisms inthepathogenesis 1. Science andTechnology (MZT) Projects supportedbytheMinistryof Figure 4. PROJECTS Together withtheMedicalSchoolat cancer anddevelopment, SonjaLevanat tumors, SanjaKapitanoviæ Koraljka Gall-Trošelj of neuroendocrinetumors, tration onVarian spectrophotofluorimeter Measurement ofplateletserotoninconcen- Science management:research Molecular 26. Epidemiology, diagnosticsandtherapy ofH. 25. Specificimmunotherapy andallergicdis- 24. The effects ofnewdrugsand hyperthermia Assessingfunctionsoftheheatrepeatin 23. Neurotransmittersinstressandregulation 22. Neuropharmacology ofserotonergicsys- 21. 20. Regulation ofectopeptidasesandopioid 19. Nonlinearmodulation of thechroniclym- 18. chiptechnologyinglobalprofilingof DNA 17. Embryoniccellproductionofpancreatic-like 16. 15. Virus Tumor genetherapy–correctionofonco- 14. Transcriptional controloflymphocytedevel- 13. Modulationofimmunologicalresponseby 12. Molecularmechanismsofimmunosuppres- 11. Cxclooxygenase-2:newtargetforchemo- 10. Geneorproteintransductionandsignalling 9. Oxidativestressandmalignantdiseases, 8. Geneticandmolecularprognosticfactorsof 7. New therapeuticpossibilitiesinbreastcan- 6. Insulin-likegrowthfactorfamilyofgenes in 5. inneuroendocrinetumors, RoleofFHIT 4. pylori infection (MiroslavaKatièiæ) eases (BranimirÈvorišèec) xenografts, MarkoRadaèiæ on thegrowthofmousetumorsandhuman Huntingtin protein,OliverVugrek receptorsinvitro,DankaPerièiæ of GABA-A tem, DoroteaMueck-Šeler ment withopioids/opiates, Tatjana Marotti Oxidative/antioxidative statusaftertreat- receptors expression,JelkaGabrilovac phatic leukemia,BrankoVitale tumors, ŠimeSpaventi islets, MirkoHadžija virus vaccine,MislavJurin suppressor genes,JasminkaPaveliæ astefania Antica opment –itsroleinleukemogenesis,Mari- bioactive peptides,BiserkaPokriæ sion, RenataNovak Radan Spaventi prevention andtreatmentofcolontumors, Krešimir Paveliæ pathways intransformedcells, Neven Žarkoviæ cervical cancerogenesis,MagdalenaGrce cer, JosipUnušiæ lung cancer, LjubomirPaveliæ Šime Križanac antitumorous actionandoncolytic ZMM RBI annual report 2003. 55 Mol. 2003 , Int. J. Biochem. . , 607-613. , 1008-1012. 62 4 Review , 422-431. , , Scand. J. Immuno. , 281-91. 35 24 2003 2003 , , , 2003, , 2003 , International Neuropsychiatric Pula International Neuropsychiatric EMBO Sectoral Meeting on Molecular Meeting EMBO Sectoral Urology rd nd Medicine, 19. – 22. June, 2003. Cavtat, 19. – 22. June, Medicine, Molecular Organizers: European Croatia. Ruðer Boškoviæ and Biology Organization Institute. 2003, Pula, Croatia. Symposium, 18-21.06. “Biological markers of Satellite Symposium: Organizers: Ruðer psychiatric diseases”. and Croatian Pharmaco- Boškoviæ Institute Mueck-Šeler and N. logical Society (D. Pivac). SELECTED PUBLICATIONS CONFERENCES , 588-600. 2. 43 Review articles Dikšiæ - Mueck-Šeler D. and Pivac N: Mirko In: Herak J Radiochemist and Neuroscientist. in the (ed) Distinguished Croatian Scientists (Part II), Croatian-American Society, World Zagreb, Croatia, 2003, pp 133-142. small scale: Kralj M., Pavelic K., Medicine on a EMBO Reports Grdisa M., White M. K., Molecular and biochem- Grdisa M., White M. K., Molecular and of HD3 chicken ical events during differentiation erythroblastic cell line, a bioactive Žarkoviæ, N., 4-Hydroxynonenal as marker of pathophysiological processes. Asp. Med. Antica, M., Kusic, B., B., Martinis, M., Vitale, A., Kusec, R., Jaksic, B., Rabatic, S., Gagro, Prolegomenon for chronic lymphocytic leukaemia [Review]. 1. 2 Paveliæ J., Zeljko Ž., Herak-Bosnar M., Molecular Paveliæ J., Zeljko Ž., Herak-Bosnar zone genetic aspects of prostate transition lesions. Cell. Biology 58 th European-American rd peroxidation product 4-hydroxynonenal, 39 tion and 4-hydroxynonenal in Third Interna- experimental neuropathology, Abnormal tional Symposium on Normal and Development of the Human Fetal Brain, June 13-15, 2003, Zagreb, Croatia. School in Forensic Medicine and Mayo Clin- School in Forensic Medicine and Mayo Advanced Molecular and Cellu- ic Course in lar Medicine, 1-5 September 2003, Zagreb, Croatia Congress of the Polish Biochemical Society, September 16-19, 2003, Gdansk, Poland. Medicine. The 3 Awards and about European Research and Awards Working Council. Member of Preparative of party for EMBC/EMBO on the Selection Party EMBC and Life Strategic Working Coun- Sciences in the European Research opinion. FEBS, EMBO The scientist’s cil. 2003, and UNESCO. Paris 18-19 February Paris, France diagnostics, cellular and gene therapy, diagnostics, cellular and gene Krešimir Paveliæ (Core) Marotti (BICRO) Mariastefania Antica (International Office for Antica (International Office Mariastefania Bonn, Germany) Bilateral Cooperation Milivoj Boraniæ (MZT, HITRA Milivoj Boraniæ (MZT, (TP-01/0098- 03) antirheumatic and antioxidative substances and antioxidative antirheumatic resin,from pine (MZT, Neven Žarkoviæ HITRA, TP-01/0098-28) SELECTED LECTURES 4. Žarkoviæ N: Biomedical relevance of lipid 3. Žarkoviæ, N: Oxidative stress, lipid peroxida- 2. Pavelic K: Nanotechnology and Molecular 1. Pavelic K: Opinion on EMBO Research 2. Center for integrative genomics, molecular 1.Tatjana Ecopropolis - natural antioxidant, Other projects 3. snRNPmalignancies, U2 in lymphoid 2. skin transplants in vitro, Production of 1. new analgetic, of Development Research and development projects development and Research Additional publications Kowanetz K, Szymkiewicz I, Haglund K, Kowanetz M, Husnjak K, Taylor JD, Soubeyran Paveliæ K., Kolak T., Kapitanoviæ S., Radoševiæ P, Engstrom U, Ladbury J, Dikic I. Identification S., Spaventi S., Krušlin B., Paveliæ J.: Gastric of a novel proline-arginine motif involved in cancer: the role of insulin-like growth factor 2 CIN85-dependent clustering of Cbl and down- ZMM (IGF 2) and its receptors (IGF 1R and M6-P/IGF regulation of EGF receptors. J. Biol. Chem. 2R). J Pathol., 2003, 201, 430-438. 2003, 278, 39735-39746.

Kralj M., Husnjak K., Korbler T., Paveliæ J.: Schroeter E.H., Ilagan M.X.G., Brunkan A.L., Endogenous p21WAF1/CIP1 status predicts the Hecimovic S., Li Y-M, Xu M., Lewos H.D., Saxe- response of human tumor cells to wild-type p53 na M.T., De Strooper B., Coonrod A., Tomita T., and p21WAF1/CIP1 overexpression. Cancer Gene Iwatsubo T., Moore C.L., Shearman M., Goate Ther., 2003, 10, 457-467. A., Wolfe M.S., Kopan R., A presenilin dimmer at the core of the gamma-secretase enzyme: Pivac N., Mueck-Šeler D., Diksic M.: Effects of insights from parallel analysis of Notch 1 and desipramine on regional serotonin synthesis rate APP proteolysis. Proc. Nat. Acad. Sci. USA, in the rat brain: Acute and chronic autoradi- 2003, 100, 13075-13080. ographic studies Neurochem Int., 2003, 43, 611- 619. Kušiæ, B., Dominis, M., Dzebro, S., Antica, M., Molecular insight into the diagnosis of lym- Lijkiæ I., Biðin Z., Pokriæ B.: Differentiation of phoma. Int. J. Mol. Med., 2003, 12, 667-71. infectious bursal disease viruses in Croatia. Avian Dis. 2003, 47, 59-65. Miloševiæ, D., Batiniæ, D., Konjovoda, P., Blau., N., Štambuk, N., Nižiæ, Lj., Vrljièak, K., Batiniæ, Perièiæ D., Swim stress inhibits 5-HT2A receptor- D., Analysis of calcium, oxalate, and citrate inter- mediated head twitch behaviour in mice. Psy- action in idiopathic calcium urolithiasis in chil- chopharmacology, 2003, 167, 373-379. dren. J. Chem. Informat. Comp. Sci., 2003, 43, Erster S., Slade N., Moll U. M., Mutational analy- 1844-7. sis of p53 in human tumors: direct DNA sequenc- Saboèanec, S., Balog, T., Šverko, V., Marotti, T., ing and SSCP. Methods Mol. Biol., 2003, 234, Sex-dependent antioxidant enzyme activity and 219-30. lipid peroxidation in ageing mouse brain. Free Dogan Koružnjak J., Grdiša M., Slade N., Zamo- Radical Res., 2003, 37, 743-8. la B., Paveliæ K., Karminski-Zamola G., Novel Antica, M., Kusic, B., Hranilovic, D., Dietz, AB., derivatives of benzo[b]thieno[2,3-c]quinolones: Vuk-Pavlovic, S., Cloning the cDNA for murine synthesis, photochemical synthesis, and antitu- U2 snRNP-A' gene and its differential expression mor evaluation. J Med. Chem., 2003, 46, 4516- in lymphocyte development. Immunol. Let., 24. 2003, 82, 217-223. Šariæ T., Muller D., Seitz H. J., Paveliæ K., Non- covalent interaction of ubiquitin with insulin- degrading enzyme. Mol. Cell Endocrinol, 2003, 204, 11-20. RBI annual report 2003.

56 Division of Marine and

Environmental ZIMO Research

http://www.irb.hr/str/zimo

DIVISIONAL ORGANISATION D Laboratory for aquaculture and fish Head: until May 2003, Božena Æosoviæ, pathology, Emin Teskeredžiæ from May 2003, Tarzan Legoviæ D Laboratory for ecological modelling, Vera Žutiæ

The Division of Marine and Environmental Research (ZIMO) consists of the following OVERVIEW OF THE DIVISION laboratories: During 2003, researchers from the division worked on over 50 research projects D Group for satellite oceanography, contracted by the Ministry of Science and Milivoj Kuzmiæ Technology, as well as outside clients. These projects spanned a wide range of top- D Group for modelling and information ics in marine and environmental science, systems, Ivica Ružiæ ranging all the way from satellite oceanogra- D Laboratory for aquatic physical phy, on the large scale, down to nanotech- chemistry, Božena Æosoviæ nology, at the other extreme. Each project D Laboratory for radioecology, was designed to contribute to the overall Stipe Luliæ mission of the division, which is to continue D Laboratory for chemistry of trace to strive for excellence in the fundamental metals, Goran Kniewald and applied research of environmental sys- D Laboratory for electrochemistry and tems, processes and states. In this way, surface chemistry, Marijan Vukoviæ ZIMO aims to make a significant contribution 2003. report annual RBI D Laboratory for biogeochemistry of towards achieving the optimal management organic compounds, Marijan Ahel of the environment, for the benefit of our country and, indeed, the whole world. D Laboratory for biological effects of metals, Biserka Raspor

D Laboratory for molecular ecotoxicology, Sanja Krèa

57 58 RBI annual report 2003. ZIMO cations, junctions", cal processesatwell-definedthree-phase and anewmethodtostudyelectrochemi- fer ofionsacrossliquid-liquidinterfaces newaccesstoGibbsenergiesoftrans- "A F. Scholz,Š.Komorsky-Lovric,M.Lovric ampton, totheauthorsofpublication: during Electrochem2003inSouth- paper intheyear2000waspresented, cations Award 2003forthebestcited Award 2003 Electrochemistry Communications water andtodistinguish betweenfreshly the relativeacidityoforganic matterinsea- researchers, forthefirst time,todetermine Adriatic Sea. This methodenabled properties oforganicmatterintheNorthern was usedinthestudyofsurfaceactive newelectrochemicalmethod A the Danuberiver. navigation mapfortheCroatiansegmentof through thecreationofavectorizeddigital way Informationsystemwasachieved Information system Development oftheCroatianFairway chemistry Communications er.com./locate/elecom (seealso, journal's homepageathttp://www.elsevi- awarded paperisfreelyaccessibleviathe The ElectrochemistryCommuni- ACHIEVEMENTS TOP novel applicationofelectrochemistry A Further developmentoftheCroatianFair- 2000 Electrochemistry Communi- , 2 , 112. The full-textofthe 2003 , Electro- 5 ). real-time signalizationofdepth. Figure 1 ference fromtrawlersisnegligible. left: model toestimatedepositionofparticlesfromanaquaculture. Acoustic Dopplercurrentprofileranchoredinawaythatinter- indicator ofcurrentsfromIoneanintothe Adriatic sea. pute currentsanddispersionfromcoastalsources. Filling the Adriatic mapwithvariable-depthprismstocom- last decade. alkylphenol polyethoxylate type,duringthe concentration ofnon-ionicsurfactants,the and indicatedasignificantdecreaseofthe pounds inthehighlystratifiedKrkaestuary biogeochemical behaviourofthetargetcom- The methodswereappliedtoinvestigatethe spectrometry (MS/MS)hasbeendeveloped. chromatography (HPLC)andtandemmass products usinghigh-performanceliquid ionic surfactantsandtheirstablemetabolic the tracedeterminationofanionicandnon- concentrations Comprehensive analysisofsurfactant more refractivehumicsubstances. produced biogenicorganicmatterandthe . Above left: . Above comprehensiveanalyticalapproachfor A Software forDanuberivernavigationwith Above right: Below right: Temperature asan Result ofa3-D Center: Below ZIMO RBI annual report 2003. 59 The expression of multidrug resistance- of multidrug The expression of the mineralogical and An investigation In the context of a bilateral cooperation The Extension of the Croatian jurisdiction Evidence for drug resistance in mullets resistance drug for Evidence to related proteins (MRPs) associated mRNA isolated from the liver tissue in has barbatus) red mullet (Mullus teleost cDNA This novel partial been found. mullet represents the first sequence of red reported for an MRP-related sequence species. aquatic teleost fish origins of historical Uncovering the artefacts 2 chemical characteristics of the Lamboglia under- amphorae, excavated from different Adriatic Sea water localities in the central This study was used to was carried out. and resolve the origin of the raw materials, to indicate the probable loca- consequently, tion of historical production facilities. The mesocosm experiment (USA), with National Science Foundation interactive ZIMO and CIM-Rovinj performed and and simultaneous data collection experiment exchange for the mesocosm S. (http://nereus.irb.hr/lem/theexperiment, Svetlièiæ, scientific V. Geèek, web-master, coordinator) Adriatic Sustainable management of the Sea Adriatic Sea was approved by the in the Croatian Parliament on October 3, 2003. This decision, in agreement with the UN Convention on the Law of the Sea, was unique properties Marine lake: Investigation of its Effects of heavy metals on organisms Effects of heavy metals Figure 3. Two bacterial communities expressing bacterial Two Figure 2. Polychlorinated Biphenyl (PCB) transforma- Polychlorinated Biphenyl (PCB) laboratory tion activity have been found in polluted enrichment experiments from PCB miner- soils. In addition, substantial atrazine communi- alization activities of four bacterial soil and ties were found in enriched polluted wastewater. Bacteria for waste disposal 60 RBI annual report 2003. ZIMO .Omanoviæ D.,Pižeta I andBranicaM., 5. Legoviæ T. RevisitingtheRedfieldratioand 4. .Legoviæ T., GraduateEnvironmentalMana- 3. T. T. ed totheMinistryofForeign Affairs (Legoviæ need forsustainablemanagementpresent- based ontheproofofoverfishingand .Komorsky-LovriæŠ.Voltammetric determi- 2. Horvat-RadoševiæV., Electrochemical 1. Figure 4. Croat. Int.Rel.Rev INVITED LECTURES ization ofnatural organicmatter–metal Pseudopolarography asatoolforcharacter- Niva, Tromso, 2. April 2003. ed CoastalZoneManagement. Aquaplan marine environment,Workshop onIntegrat- modelling theeffects ofafishcultureon tors, Washington, USA,1.Feb.2003 Council ofEnvironmentalDeansandDirec- cation forSustainableandSecureFuture. Science, PolicyandtheEnvironment:Edu- gement Study. 3 15. -19.June2003. and theirmodels, Trešt, CheckRepublic, sion: Electrochemistryofbiologicalsystems graphite electrode.36 some localanaestheticsimmobilizedonthe nation ofmicroparticlescocaineand of Sciences,Sofia,Bulgaria,2003. mical PowerSources,Bulgarian Academy Days, CentralLaboratoryofElectroche- metal electrodes,2003SofiaImpedance impedance spectroscopyofsomenoble Monitoring fishhealthandassessmentof environmental effects rd National Conferenceon ., 2003 th Heyrovsky Discus- , 9 , 35). .SvetlièiæV., Frommicroparticlestogiant 6. .8 1. .Physical chemistryandbiogeochemistryof 3. Analysis andbiogeochemistryoforganic 2. Tidal andlonger-period dynamicsofthe 1. Science andTechnology (MZT) Projects supportedbytheMinistryof BiogeochemicalProcessesin Anoxic 6. RovinjOceanographicWorkshop, Office of 5. Aquaculture,CoastalManagementand 4. Rovinj2003Experiment,Northern Adriatic 3. Workshop ontheContentofStrategic 2. ORGANIZED CONFERENCES PROJECTS gels ofnorthern Adriatic, 13 et duVar. LaGarde,France :9-10Oct.2003,Universitéde Toulon » isa Monal- du GroupementdeRecherche« ions interactions,Séminairedelancement Turkey, 11-13. October, 2003. chemical BiophysicalDays,Kusadasi, Marko Branica trace metalsinaquaticsystems, Marijan Ahel compounds intheaquatic environment, northern Adriatic, Milivoj Kuzmiæ (I. Cigleneèki-Jušiæ) Workshop, Zagreb,January, 17-20.2004 Marine Environments,Croatian-British Perkins, R.Signell) Rovinj, 7-9October2003(M.Kuzmiæ,H. Naval ResearchInternationalFieldOffice, Croatia (B.Æosoviæ) gian, Workshop, Vodice, October1-5, Environmental Impact,Croatian-Norwe- 2003 (Vera Žutiæ) Boškoviæ Institute,Rovinj,11-13 August, Mesocosm, InternationalWorkshop, Rudjer (T. Legoviæ) and PhysicalPlanning,Zagreb,28.04.2003 Adria, MinistryofEnvironmentalProtection Environmental Impact Assessment -Družba 2003 OceanographicWorkshop (B.Raspor) for wateranalysis,Rovinj,March11-14, th Meeting oftheLaboratoriesaccredited th Balkan Bio- ZIMO RBI annual report 2003. 61 EMCO (EU-FP6) (M. Ahel) (M. (EU-FP6) EMCO , agement and Protection of Resources. EU and Protection of agement Coor- Raspor, (B. FP6-2002-INCO-WBC-1. WP3) dinator of The and determination, ganese speciation Royal Society,(Š. Komorsky- 2003-2005. Oxford University). Lovriæ and R. Compton, Luliæ) Management, 2003 ( S. Water Krško, Slovenija (S. Nuclear Power Plant Luliæ) water ecosystems from atmosphere and in the waste materials generated by warfare (M. (APOPSBAL) Yugoslavia area of former Picer) contam- matter in speciation of bioavailable inants in coastal waters, French-Croatian Cooperation, (G. Kniewald) Adriatic Sea, Bilateral Cooperation Northern USA, Scripps Institution of project with NSF, Žutiæ), UCSD (V. Oceanography, Legoviæ) culture, ECASA, EU-FP6-2003 (T. Ahel, B. Æosoviæ, M. Kuzmiæ, D. Hršak, (M. E. B. Raspor, B. Pivèeviæ, M. Picer, Žutiæ) V. Teskeredžiæ, Kozarac, D. Hršak, B. Raspor) estuarine system for Croatian freshwater, and coastal marine areas. Croatian-Norwe- gian International project (Coordinators: S. Britviæ, B. Hylland, Ketil, B. Raspor, G. Klobuèar) Hackenberger, Traffic. and Ministry of Seafare Dunav. Cooperation with the European program COMPRIS and Regional Project D4D (I. Ružiæ) treatment of municipal and industrial and municipal of treatment wastes OTHER ACHIEVEMENTS 6. Use, man- River Basin: Sustainable Sava 7. man- Environmental sono-electroanalysis: 8. Croatian Monitoring of Dunav water quality, 9. monitoring around Krško, Radiological 10. in the Assessment of selected POPS 11. natural organic The role of MONALISA, 12.mucilage formation in the Mechanism of 13.Aqua- Approach for Sustainable Ecosystem 14. programme Croatian national monitoring 1. Æosoviæ, Z. Reference laboratory (B. 2. An integrated environmental monitoring 3. Digital navigational charts for the river Emerging Contaminants, through advanced role of enzymes in the formation of inorganic colloids), Croatia-Slovenia Collaboration Project (Ivan Sondi) cal sensor and measurement system for Žutiæ reactive microparticles (HITRA), Vera of effluents from atrazine production of effluents (HITRA), Dubravka Hršak techniques (HITRA), Ivan Mijatoviæ and Marijan Ahel biomarker of environmental quality, quality, biomarker of environmental Tvrtko Smital transformation processes, Dubravka Hršak transformation processes, Dubravka Tarzan Legoviæ Tarzan mentary systems of the Adriatic platform, mentary systems of the Goran Kniewald some coastal area of Dalmatia, Mladen Picer Biserka Raspor Delko Barišiæ Vera Žutiæ Vera faces in the environmental protection, faces in the environmental Marijan Vukoviæ culture receiving waters, Emin Teskeredžiæ Emin culture receiving waters, protection and navigation management, and navigation protection Ivica Ružiæ electrolytes, Milivoj Lovriæ electrolytes, in marine and environment, and in marine Božena Æosoviæ 5. Reduction of environmental risks, posed by 4. nano-tecnology (The Nano-systems and 3. Development of new type of electrochemi- 2. Conceptual model for biological treatment 1. of wastewaters using membrane Treatment Selected research and development projects Selected research and development 16. mechanism as a Multixenobiotic resistance 15. as catalysts in bio- Microbial communities 14. ecosystems, Modelling aquatic 13. recent and ancient sedi- Geochemistry of 12. pollutants in Persistent organohalogen 11. biomarkers, Metals and cellular 10. systems, Radionuclides in environmental 9. processes and eutrophication, Interfacial 8.metal sur- Preparation and properties of 7. in aqua- Protection of biocoenotic balance 6. for environmental and info. systems Models 5. solid in liquid and research Electroanalytical 4.organic substances of and reactivity Nature 62 RBI annual report 2003. ZIMO mucilage. mation bysulfideindiatomcultureandnatural Pepi M.,BaldiF. Mucopolysaccharidetransfor- Cigleneèki I.,PlavšiæM.,Vojvodiæ V., ÆosoviæB., 2003 Modelling Journal Ecological ModellingConference, Legoviæ T. (Editor),ISEM The thirdEuropean Arrivalof AFM equipmenttothedepart- 5. Developmentofcontrolsystemwithnaviga- 4. bilised onthegraphiteelectrode. some localanaestheticsandantithusicsimmo- Voltammetric determination ofmicroparticles Komorsky-Lovriæ Š.,Vukašinoviæ N.,PenovskiR. 227-239. sis Estuarine CoastalandShelfScience Calcareous EstuarineandMarineSediments. of Image-basedGranulometrytoSiliceousand Balagurunathan Y., DoughertyE.R. Application Franèiškoviæ-Bilinski S.,BilinskiH.,VdoviæN., American mineralogist sheet boratetuzlaite,NaCa(B Thermal stabilityandvibrationalspectraofthe Bermanec V., FuriæK.,RajiæM.,KniewaldG., Zagreb, Experimental Data.RudjerBoskovicInstitute, Experiment, Northern Adriatic Mesocosm: Svetlièiæ V, Hoziæ A. (Editors.)Rovinj2003 Journal ofElectroanalytical Chemistry reactions ataconicfilmthree-phase electrode. grams ofsimultaneouselectron andiontransfer Lovriæ M.,ScholzF. Modellingcyclicvoltammo- 540 . SELECTED PUBLICATIONS , 89-96. 2003 material science.(V. Svetlièiæ,V. Žutiæ). research activitiesrangingfromlife-to far thefirstinCroatia,willbeapplied ment. Itisexpectedthattheequipment,so (I. Ružiæ) Ministry ofSeafare, Traffic andConnections cooperation withKOMTEH,Zagreband tional signsattheriverDunav. In , 263 2003 , , 17-27. 15 Marine EcologyProgressSeries , ISBN953-6690-46-2. , 544-547. , 2003 . 2003 , 170 , , 170-490. 88 5 O , 271-276. 8 (OH) Electroanaly- . Ecological 2003 2 )x3H . 2003 , 2 58 O. , . , Science Cres Island,Croatia. troanalytical Chemistry isotherm intheCd concentration ontheparameters oftheFrumkin Zeliæ M.,LovriæM. The influenceofelectrolyte northern Adriatic Sea. northern Adriatic sures fromanareainfluencedbymucilage:the solved organicmatterinnutrient-enrichedenclo- M., Cauwet,G.Changesinparticulateanddis- Malej, A., Mozetiè,P., Turk, V., Terziæ, S., Ahel, Research urements. microelectrodes forinsituvoltammetricmeas- Pižeta I.,BillonG.,FischerJ-C.,Wartel M.Solid 543 cathodic strippingvoltammetry. Mlakar, M.Determinationoftraceytterbiumby Journal ofElectroanalyticalChemistry measurements ofreversibleelectrodereactions. of tracemetals.Part1. The automatic ASV Omanoviæ D.,BranicaM.Pseudopolarography 2003 Aquatic Toxicol. freshwater musselDreissenapolymorpha. lytical Letters particles withmetalionsinnaturalwaters. for studyingtheinteractionoforganicmatterand Plavšiæ M.Electroanalyticaltechniquesapplied 1396. iiserythrophtalmusL dinius and nutritivevalueofgutcontentsrudd( Tomec M., Teskeredžiæ Z., Teskeredžiæ E.Food Interface Science form SilverNanoparticles. Highly ConcentratedStableDispersionsofUni- Sondi I.,GoiaV.Dan, MatijeviæE.Preparationof marine mussel mechanism (MXR)transportactivityinthe Inducibility ofthemultixenobioticresistance Smital T., SauerbornR.andHackenbergerK.B. , 83-92. , 15 . , 27-35. 2003 . 2003 Electroanalysis . , 2003 48 Mytilus galloprovincialis , 2003 25 . , 28-34. 2+ 2003 , , 949-966. - I 36 , Czech Journalof Animal 65 - . , 143-157. , system. 2003 260 , 443-465. Journal ofPlankton .) fromLakeVrana, Journal ofColloidand . , 75-81. , 541 2003 Journal ofElec- Electroanalysis , 67-76. , 15,1389- . and the 2003, Scar- Ana- . Center for Marine CIM Research

http://www.irb.hr/str/cim

DEPARTMENT ORGANISATION tions concerning environmental protection. Head: Nenad Smodlaka This project potentially represents an important basis for the regional initiative, recom- The Center for Marine Research (CIM) mended by the Croatian-Italian-Slovenian consists of the following laboratories: trilateral commission for the protection of the Adriatic, of establishing a permanent D Laboratory for ecology and observing system (Coordinated Adriatic systematics, Ana Travizi Observing System - CAOS), suitable for D Laboratory for processes in the future marine research in the Adriatic. The marine ecosystem, Danilo Degobbis international projects ADRICOSM (2002- D Laboratory for ecophysiology and 2004) and MAT (1999-2003) are closely toxicology, Èedomil Lucu linked with the project “Jadran”.

D Laboratory for marine molecular toxicology, Renato Batel Maritime Forecasting system The project “ADRICOSM,” an internation- D Laboratory for ecotoxicology, Bartolo Ozretiæ al collaboration between Italy, Croatia and Slovenia, has put in place, for the first time, a near real-time maritime forecasting system OVERVIEW OF THE CENTER for the Adriatic Sea. The basis for this modelling and predictive forecast system has, as Project Jadran one of its sources, a recent high resolution

The Ruðer Boškoviæ Institute (RBI), and oceanographic study of the north-eastern 2003. report annual RBI in particular the Center for Marine Research Adriatic conducted by RBI-CIM in the west (CIM), is responsible for coordinating the Istrian coastal region (Figure 1: Circulation national monitoring and research aspects of pattern). Previously unknown details of cir- the project “Jadran”. In 2003 CIM enhanced culation patterns have been revealed and the level of cooperation among various inter- the subsequent establishment of a link to est groups and continued to contribute to the meteorological conditions is expected to fulfilment of Croatia’s international obliga- provide a basis for a future rapid marine forecast and management system.

63 Mucilage events In the framework of the project “MAT” (Mucilage phenomenon in the Adriatic and

CIM Tyrrhenian seas), numerous Italian and three Croatian research groups studied (field and laboratory work) the processes, mechanism and consequences of the mucilage events. CIM coordinated the Croa- tian research units and participated in the coordination activities of the entire project. A major part of the results obtained will be

Figure 2. Mucilaginous cloud, 14 July, 20 km off the Po River delta

TOP ACHIEVEMENTS

Invasive seaweed effects Caulerpa taxifolia is known to out-com- pete native seaweeds and seagrasses, dis- place invertebrate communities, and seri- ously reduce species abundance and diver- sity of benthic habitats. Formation and development of a specific epibionthic com- munity whose appearance and existence are closely linked to the establishment and maintenance of C. taxifolia settlement, has been described.

Figure 1. Circulation pattern New nematoda species Free-living Nematoda species, new to published in a special issue of The Science science, were discovered in the northern of Total Environment. The occurrence of a Adriatic Sea at depths of 4-47 m and in var- mucilage event in the northern Adriatic dur- ious sediment types. In particular, two new ing summer 2003 (Figure 2 - mucilaginous species of the genus Metacyatholaimus cloud, 14 July, 20 km off the Po River delta) were described as can be seen in the was monitored in real time, comparison with accompanying key.

RBI annual report 2003. laboratory data was made as well studies of the consequences of the event on the ecosystem.

64 CIM RBI annual report 2003. 65 was investigated. Studies of KRS_SD induction, its phos- Studies of KRS_SD the com- The osmoregulatory capacity of INVITED LECTURES the ecosystem, has been assessed. Investi- been assessed. has the ecosystem, it have shown flow cytometry using gations for the determi- powerful technique to be a cycle characteris- alterations in cell nation of DNA hemocyte tics in mussel 5). (Figure response Proapoptotic stress proteolytical cleavage dur- phorylation and TBTing cells stress suggest that in sponge mechanism similar to that there exists a cells where KRS/MSTpresent in human pro- in promotion of apop- tein kinase is involved 6). tosis following oxidative stress (Figure Lobster osmoregulatory capacity Adriatic lobster mercially important northern Homarus gammarus natural lob- Under extreme conditions, the Adriatic ster population living in the northern low saline coastal areas can be exposed to first time waters for short periods. For the enzyme under laboratory conditions, the be activat- carbonic anhydrase was found to respiration ed in the tissues responsible for the adapta- and osmoregulation, following concentration of lobsters to dilute seawater tions. Èedomil Lucu: Physiological adaptive mechanisms of organisms in marine and brackish water and carbonic anhy- environment. Na,K-ATPase drase. Universite degli Studi di Firenze, Diparti- mento di Chimica, Consorzio per lo Svillupo dei Sistemi a Grandi Interfasi. Florence, 28 February 2003. Metacyathlaimus papillatus nov, Male, total view nov, Metacyatholaimus adriaticus sp. sp.nov. Male, total view sp.nov. Figure 3. Figure 4. . sponges, mussels), and the estimation Studies of the Fast Micromethod® and its Studies of the Fast Micromethod® e.g of directional changes and harmful effects in effects of directional changes and harmful applicability for the measurement of the applicability for the measurement DNA of small samples for genotoxi- integrity the effects city assessment (biomonitoring), taxa or ses- of genotoxins on lower marine sile invertebrates in the marine environment ( Fast genotoxicity biomonitoring CIM

Figure 5. M. galloprovincialis DNA profiles of mussel hemocytes encountered in flow cytometric analysis: (A) normal DNA profile, normal sample 2 N peak used in statistical comparison of CV values lower then 8 %, (B) DNA profiles with CV values greater then 8 % (C) DNA profiles with aneuploid peak before 2 N peak, (D) apoptotic DNA profile, (E) asymmetric 2 N peak. F1-F5: other altered DNA profiles occurred in hemocytes of mussel natural populations.

PROJECTS 5. Ecophysiological studies and stress response in marine organisms, Projects supported by the Ministry of Èedomil Lucu Science and Technology (MZT) Other projects 1. Systematic research of the Adriatic Sea as a base for sustainable development of the 1. Mineral composition and stabile isotopes in Republic of Croatia, project “Jadran” Croat- shellfish shells as pollution indicators, ian national monitoring programme, Direc- Davorin Medakoviæ (bilateral collaboration tor Nenad Smodlaka. with Slovenia) 2. Mechanism of long-term changes in the 2. Regional collaboration in environmental Adriatic Sea ecosystem, Danilo Degobbis monitoring and forecasting in the northern 3. Programmed biosynthesis and genotoxic Adriatic Sea, Nenad Smodlaka (quadrilater- risk assessments, Renato Batel al collaboration with U.S.A., Italy and Slove- 4. Physiological and biochemical indicators of nia) toxicological stress in marine biota, 3. Adriatic Sea integrated coastal areas and Bartolo Ozretiæ river basin management system pilot proj- RBI annual report 2003.

Figure 6. Alignment of C-terminal regions of sponge and human MST homologues (MST-1/KRS-2, accession number Q13043, MST-2/KRS-1, accession number Q13188). Caspase-3 recognition sites are underlined.

66 CIM CIM RBI annual report 2003. 67 J. , 79- 287 , , 73-81. 57 2003 , , 239-252. , by dilute seawater 297 2003 Mytilus galloprovin- Croatia. Aquat. Croatia. Aquat. Tox- , , , 361-376. 2003 65 : proteolytical cleavage and : proteolytical cleavage Comp. Biochem. Physiol., , 2003 , 121-129. 64 J. Exp. Mar. Biol.J. Exp. Mar. Ecol. Helgol. Mar. Res. Helgol. Mar. , 195-214. , Homarus gammarus 135 , ) off the Adriatic coast, Adriatic the ) off 2003 . SELECTED PUBLICATIONS SELECTED Bihari, N., Mièiæ, M., Batel, R., Zahn, R.K. Flow R., Zahn, R.K. Mièiæ, M., Batel, Bihari, N., detection of DNAcytometric cell cycle alterations ( of mussels in hemocytes cialis icol Fafanðel, M., Müller, W.E.G., Batel, R. Molecular Batel, W.E.G., Fafanðel, M., Müller, TBTresponse to stress in marine sponge Suberites domuncula KRS_SD protein kinase. phosphorylation of Biol. Ecol. Mar. Exper. Jakšiæ, Ž., Batel, R DNAJakšiæ, Ž., Batel, integrity determination by Fast Micromethod®. in marine invertebrates Aquat. Toxicol. of in gills D. Na,K-ATPase Towle, Lucu, È., aquatic crustacea. 2003 R., Fuks, Luèiæ, D., Njire, J., Moroviæ, M., Precali, in the D., Bolotin, J. 2003. Microzooplankton Adriatic Sea from open waters of the northern nauplii 1990 to 1993: the importance of copepod densities. Pavièiæ-Hamer, D., Devescovi, M., Lucu, È. Acti- D., Devescovi, M., Lucu, È. Pavièiæ-Hamer, of the vation of carbonic anhydrase in tissues lobster exposure. 92. northern Adriatic Sea: A component of ecologi- CREICO (Cooperative research on NSF), cal interactions in the coastal ocean, Žutiæ (quadrilateral collaboration with Vera U.S.A., Italy and Slovenia) the effects pollution, Renato Batel (bilateral pollution, Renato Batel the effects Germany) collaboration with mariner Schwaemme sowie Schwamm- mariner Schwaemme (Biotec- assoziierter Mikroorganismen Schwaemme aus Rovinj Marin), Project: und (Kroatien) – Extractbereitstellung Renato Batel (bilateral collabora- Marikultur, tion with Germany) mucillagini nell’Adriatico e nel Tirreno e nel nell’Adriatico mucillagini collabora- Degobbis (bilateral Danilo (MAT), Italy) tion with ect (ADRICOSM), Nenad Smodlaka Nenad (ADRICOSM), ect with Italy, collaboration (quadrilateral Slovenia) France and 7. Mechanism of mucilage formation in the 6. of Biosensor methods for the assessment 5. Wirkstoffe Molekulare Biotechnologie und 4. delle e studio Progetto di monitoraggio

Center for Informatics CIR and Computing

http://www.irb.hr/cir

distributed computing, cluster computing, ORGANISATION OF THE CENTER and GRID systems. Head: Karolj Skala The CIR played an important role in the development and maintenance of the Insti- The Center for Informatics and Computing tute’s computational infrastructure. In partic- (CIR) consists of the following sections: ular, services such as server park for infor- D The optoelectronics and hypermedia mation services, numerical cluster computa- laboratory, Karolj Skala tion, printing, plotting, e-mail and internet D Information and Computer access were provided. The Institute’s local Technology research and area network and associated equipment, development, Zorislav Šojat which connects computers throughout the D Information systems, Neven Kmetiæ whole Institute, was also under the auspices of CIR, Figure 2. D Service and maintenance, Ratko Mileta TOP ACHIEVEMENTS

Development of new technologies and equipment OVERVIEW OF THE DEPARTMENT New design, implementation and testing In 2003, CIR focused on applied scienti- of the new hybrid fiber optical cable for fic and technological research as well as monomodal and multimodal optical signal

Information and Computer Technology (ICT) transmissions which is now in production in 2003. report annual RBI advancement. The center was heavily ELKA d.d. involved in software implementation and Research and development of the free development as well as the advancement of space laser communication equipment for computer usage in scientific work metropolian area networks was undertaken (e-Science technology). Further develop- and the successes presented (E. Wendling, ment was carried out in the areas of detec- K. Skala, 26th International MIPRO Conven- tion theory, modern network technology and tion), Figure 3.

69 70 RBI annual report 2003. CIR ings ELMAR, land andatsea(K.Skala for pilotwirelessLANapplications,bothon Content ManagementSystemsaswell New software were constantlyprovided. and theenhancementsinclustercomputing computing infrastructurewasalsoimproved computer network. The existingdistributed gigabit Ethernetopticalandwireless CIR plannedandimplementedthecampus Infrastructure improvements for theEuropeanproject SEE-GRIDaspart ect initiativeandcompleted thenegotiations Project initiatives Ltd. Intelligent MediaCommunications tive ofdistributedmediaservertechnology, CIR designedthesoftwareforWeb CIR startedtheCROGRID nationalproj- Figure 1. 2003 , 317.D.Kolariæ Young CIRstaff,meanage32 2003 , 345). et al. et al. , Retrospec- , Proceed- , Kluwer to establishe-Sciencetechnologies. pean andworldwideGridinitiatives,inorder pean countries,inthecontextofpan-Euro- of thisprojectistocombinewithotherEuro- of theSixthFrameworkProgram. The goal .Jointproject withtheInstituteforSoftware 2. COST-276 “InformationandKnowledge 1. International projects RemotemonitoringandcontrolofCluster 1. Information technologyproject CampusLocal Area computernetworkproj- CROGRID 4. Applications, STRIP, CROGRIDInfrastructure, 3. Freespacelaseropticalcommunications, 2. 1. Technological projects 1. StohasticSourceDetectionanddistributed Scientific projectin Applied Science Coordinator oftheprojects:KaroljSkala nology (MZT)andEuropeanUnion(EU) Supported byMinistryofScienceandTech- ic coast. regatta University andInstitutesatthe yearly gatheringofscientistsailorsfromthe Convention MIPROinOpatija. The traditional media andGridSystems CIR organizedtheannualconference CONFERENCES AND SPORTINGEVENTS PROJECTS ing Application”. gies for Advanced ScientificandEngineer- tria: “DevelopmentofNew Grid Technolo- Science fromtheUniversity ofVienna, Aus- nication”. Management forIntegratedMediaCommu- computers. ect andrealization. information processing(2002-2005). was alsostagedbyCIRinZadar, Adriat- within theInternational University sailing Hyper- CIR RBI annual report 2003. 71 , 2003 , Pro- , 173-177. Retrospective Determination . , University text 2003 International Con- th 2003 , 317-330. , 26 Free Space Optical Link , editor Mohamed Najim, 2003 , 287-291. (project HITRA) Int. conf. Information Technology Int. conf. Information 2003 Proceedings ELMAR 2003, th Free space optical communication link Free space optical communication Optoelektronièki sustavi developed in CIR in cooperation with LAIR developed in CIR in SELECTED PUBLICATIONS Figure 3. Interfaces, ITI, Skala, K. Skala, K., Zelenika, H., Nikoliæ, I. Intelligent of distributed media server technology, Media Communications Kluwer Ltd., London, Skala, K. E., Wendling, Remote Cluster Towards vention MIPRO, Hypermedia and Grid Systems vention MIPRO, Hypermedia and Grid Conference Proceedings HGS, Kolariæ, D., Dubraviæ, A., Skala, K. Kolariæ, D., Dubraviæ, Flame in of the Space characteristics of the Signal, Video 345-349. Pavkoviæ, N., Skala, K. T., Žiha, K., Stankoviæ, Handling Engineering Design Experience ceedings of 25 book, ISBN 953-6564-07-6, Local area network active equipment SRCE, Zagreb work development, ELKA, Zagreb GRID computing technology, HEP, Zagreb HEP, GRID computing technology, field of GRID technology, Ericsson - Nikola field of GRID technology, Zagreb Tesla, sity of Vienna, Austria sity of Vienna, University of Maribor, Maribor, Slovenia: Maribor, of Maribor, University using Multi- Interface Development “Visual Agents”. and Intelligent media technologies SEE GRID, EU F6, con. no. 002356 no. 002356 F6, con. GRID, EU SEE COLLABORATION WITH OTHER COLLABORATION INSTITUTIONS Figure 2. 5. of joint ICT Memorandum development, 4. Collaboration contract for fiber-optic net- 3. transfer of Collaboration agreement for the 2. in the Long-term contract for collaboration 1.of the Univer- Institute for Software Science 4. , Faculty of Education project with the Joint 3. Development, Infrastructure Grid Enabled

Center for Nuclear Magnetic Resonance NMR

http://www.irb.hr/nmr

supercede, the activity of the whole peptide Head of center: Dražen Vikiæ-Topiæ from which they are derived, were undertaken in the center in 2003. A detailed under- OVERVIEW OF THE CENTER standing of this phenomenon requires the The International Opening of the NMR determination of subtle sense-antisense center, the first and only academic NMR interactions between short peptide frag- facility in Croatia, took place in October ments with five to thirteen amino acids. Fur- 2003. Since this time, extensive services thermore, it is neccesary to perform such have been provided for scientists and analysis both in solution, via NMR spec- researchers from the Ruðer Boškoviæ Insti- troscopy, and in the gas phase, via mass tute, from the Universities of Zagreb, Rijeka, spectrometry (N. Štambuk et al., to be pub- Split and Osijek, as well as governmental lished). and industrial institutions. Research work at PROJECTS the NMR center includes bioorganic and Cooordinator of the projects: pharmaceutical chemistry as well as theo- Dražen Vikiæ-Topiæ retical calculations of structure and spectral parameters. Investigations of small pep- Domestic and international projects tides, gels and isotope effects are also in 1. Nuclear Magnetic Resonance and Calcula- progress. The center was also involved in tions of Bioorganic Molecules undergraduate and graduate studies of the 2. Multi-Dimensional NMR Spectroscopy of Biomolecules, Austrian-Croatian project Universities of Zagreb and Osijek. The center’s equipment includes 300 and 600 MHz Technological projects 2003. report annual RBI NMR spectrometers manufactured by Bruk- 1. Plant Terpenes and Lignin as Natural Sub- er Avance. strates for Biosythesis of Anticancer and Industrial Intermediates, EUREKA project. Information technology projects TOP ACHIEVEMENTS 1. Web Server with Individual Approach to Small active peptide fragments Data Base and Separated Local Network with Applications for Providing Services to Investigations of small peptide fragments NMR which are able to mimic, and even

73 Contract with industry 1. Collaboration Contract with BELUPO d. d. Pharmaceutical Industry, 2003-2004 NMR International collaborations 1. Institute of Chemistry, Johannes Kepler University, Linz, Austria 2. National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia 3. Faculty of Science, Masaryk University, Brno, The Czech Republic 4. Mayo Clinic and Foundation, Rochester, Figure 1. The NMR operators room with a view on MN, USA 300 MHz NMR magnet

ORGANIZATION OF INTERNATIONAL SELECTED PUBLICATIONS CONFERENCES AND COURSES Ž. Mariniæ, M. Æuriæ, D. Vikiæ-Topiæ and Lj. 1 13 1. Opening of the NMR Center and FT EPR Tušek-Božiæ: Assignment of H and C NMR Facility at Ruðer Boškoviæ Institute and The Data for Diethyl 2- and 8-Quinolylmethylpho- Second International DU NMR Conference, sphonates and their Palladium(II) Dihalide Com- Zagreb, October 20-22, 2003 plexes. Magn. Reson. Chem. 2003, 41, 969- 2. The 2nd Central European Chemical Meet- 973. ing, Varaždin, June 19-21, 2003 A. Višnjevac, B. Kojiæ-Prodiæ, M. Vinkoviæ, K. Mli- 3. Summer School on Biomolecular Structure nariæ-Majerski: Dispiro [Adamantane 2,2´- and Dynamics - Advances in NMR and 1´,3´,6´,9´,11´,14´-Hexathiacyclohexadecane- Computational Methods, Otoèec, Slovenia, 10´,2”-Aadamantane]. Acta Cryst. C., 2003, 59, June 22-29, 2003 314-316. 4. The 18th Dubrovnik International Course & Conference on the Interfaces among Math- ematics, Chemistry and Computer Sciences, Dubrovnik (IUC), July 23- 28, 2003 5. The Fourth International Dubrovnik NMR Course and Conference, DU NMR 2003, Dubrovnik (IUC), June 28-July 1, 2003 RBI annual report 2003.

Figure 2. The staff of NMR center with a 600 MHz NMR magnet

74 Library LIB

http://library.irb.hr

Head of Library: Jadranka Stojanovski

OVERVIEW OF LIBRARY ACTIVITIES The access to the relevant bibliographic and full-text databases was provided through the As the largest scientific library in the field of center for online databases natural sciences in Croatia, the holdings of the (http://baze.irb.hr), maintained by the RBI Institute’s library cover the fields of physics, Library for the academic and research com- mathematics, electrical engineering, chem- munity in Croatia. Databases such as Current istry, biology, medicine, environmental, and Contents, Medline, Inspec, PsycInfo, EI Vil- marine sciences. lage, Web of Science (WoS) and others are The Library’s book collection amounts to accessible on a regular basis. some 33000 volumes, 500 of which were The RBI Library has a well established inter- newly acquired in 2003. The total number of library loan service with Croatian and foreign available journal volumes is nearly 40000. As libraries. Last year it fulfilled approximately electronic access to information and journal 700 requests for documents made by RBI articles increased significantly in 2003, the members, and over 1200 requests from other journal acquisition model was rather complex. libraries. To help our users, and other libraries, It consisted of: to obtain printed and electronic documents - 96 printed and electronic journals faster and more easily, we developed a sys- funded by the RBI tem (SEND) for managing loans through a - 251 printed journals funded by Ministry of web interface.

Science and Technology (MZT) In addition, the library’s extremely popular 2003. report annual RBI - 7530 electronic journals funded by MZT web portal, with more than 2500 web pages, via consortia agreements with major sci- received over 1400 visits per day and was entific publishers regularly maintained throughout the year. - 6715 electronic journals provided through cooperation with University Library of Regensburg and their collaborative project Elektronische Zeitschriftenbibliothek EZB.

75 76 RBI annual report 2003. LIB document orderingsystem forinterlibrary The SENDproject is currentlyenjoyingarapidexpansion. the fulltextofmorethan7000journals,and its creation,thedatabaseenabledaccessto ic journalsthroughasingleinterface.Upon this projectistoprovideaccesselectron- product oftheRBILibrary. The maingoalof project The EJOL Croatia. records coveringallscientificpublicationsin raphy andcollection,withover100000 now evolvedintoacomprehensivebibliog- MZT, wasreleasedin2003.CROSBIhas ing fromresearchprojectsfinancedbythe releases dataaboutscientificpapersresult- (http://bib.irb.hr), whichcollectsandpublicly (CROSBI) Croatian ScientificBibliography ED(http://send.irb.hr)istheelectronic SEND (http://ejol.irb.hr) is thenewest EJOL The newversionofCROSBI ACHIEVEMENTS TOP Figure 1. CROSBI Interface provide comprehensivestatisticalreports. ment andprocessingofrequests,to ordering byusers,toimprovethemanage- system wasdesignedtofacilitatedocument loans. Fullydevelopedinsidethelibrary, the .Stojanovski,J.Croatian Academic andSpe- 4. Pažur, I.ElectronicJournals–Catchthemif 3. MelinšèakZlodi,I.;M. 2. Konjeviæ,S.CroatianScholarlyJournalson 1 SELECTED LECTURES Dubrovnik, Mljet,Croatia,26-30 May2003. 2003. tent //LibrariesintheDigital Age LIDA cial LibraryWeb Sites:an Analysis ofCon- of Zagreb,Croatia,8-9November2003 cal EngineeringandComputing,University FacultyofElectri- ian -howothersseeus” Libraries Conference“Don’tshootthelibrar- you Can//Croatian Academic andSpecial 2003 2003. Rovinj,Croatia,24-26November OAI@AKM // Archives, Libraries,Museums Germany, 7-9May2003 Sciences InformationManagement”.Kiel, Crossing theBoundariesin Aquatic the Web //EURASLIC10“SmoothSailing: Figure 2. JLHomepage EJOL LIB RBI annual report 2003. 77 , , 46, . Kemija u indus- 2003 . TERENA Networking , 32-41 , 123-125. Vjesnik bibliotekara Hrvatske Vjesnik 52 , 2003 2003 SELECTED PUBLICATIONS PUBLICATIONS SELECTED . (2003) 3-4, 111-118. (2003) 3-4, arhivi – revolucija Zlodi, I. Elektronièki Melinšèak informacijama? 6. semi- u pristupu znanstvenim knjižnice, muzeji : moguænosti surad- Arhivi, nar informacijske infrastruk- nje u okruženju globalne Zagreb: T.(ur.). Katiæ ture: zbornik radova, društvo, Hrvatsko knjižnièarsko Digitalni izvori znanja i njihovo korišten- I. Pažur, Instituta Ruðer Boškoviæ je - primjer knjižnice - izvori i korištenje znanja Specijalne knjižnice Zagreb: Hrvatsko knjižnièarsko Jokiæ, M. (ur.). društvo, I.; Konjeviæ, S. Kemièari instituta Ruðer Pažur, Boškoviæ i elektronièki èasopisi. triji AStojanovski, J. CROSBI - for Monitoring Tool Scientific Productivity Conference and CARNet User’s Conference 2003 Conference Proceedings Konjeviæ, S. Hrvatski znanstveni èasopisi na èasopisi S. Hrvatski znanstveni Konjeviæ, Internetu, RBI Library and its users Figure 3. (Ivana Pažur and Sofija Konjeviæ) (Jadranka Stojanovski) (Jadranka Stojanovski) Natural Sciences (Jadranka Stojanovski) (Jadranka Stojanovski) PROJECTS CONFERENCE ORGANIZATION CONFERENCE 5. EJOL Journals Online Library Electronic 4. Who is Who in Science in Croatia 3. Online Databases Center 2. Croatian Scientific Information System – 1. Bibliography CROSBI Croatian Scientific Projects supported by the Ministry of Projects supported (MZT) Science and Technology The Croatian academic and special libraries con- special libraries academic and The Croatian shoot the librarian 2003 entitles “Don’t ference of organized by the Insti- see us?” was - how others and of engineering library at the faculty tute’s from 8 - 9 Zagreb, Zagreb University, computing, November 2003. Bilješke:

The Ruðer Boškoviæ Institute (RBI) is the largest Croatian research centre in sciences and science applications. In the multi-disciplinary environment of the Institute more than 500 academic staff and graduate students work on problems in experimental and theoretical physics, chemistry and physics of materials, organic and physical chemistry, biochemistry, molecular biology and medicine, environmental and marine research and computer science and electronics. Within Croatia, the RBI is a national institution dedicated to research, higher education and provision of support to the academic communi- ty, to state and local governments and to technology-based industry. Within the European Union, the RBI forms a part of the European Research Area. Worldwide, the RBI collaborates with many research institutions and universities upholding the same values and vision.